Antimicrobial actinomycins isolated by bioautography from Amazonian soil Streptomyces collinus Tü broth.

IF 1.6 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-10-08 DOI:10.1080/14786419.2025.2566906

Ingrid Reis da Silva, Yasmin Cunha da Silva, Mauricio Morishi Ogusku, Valdir Florêncio da Veiga-Junior, Rudi Emerson de Lima Procópio

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Abstract

The aim of this study was to isolate and identify bioactive compounds through a bioactivity-guided analysis of extracts from Streptomyces strains. A bioactive extract from a Streptomyces species isolated from Amazonian soil, identified as S. collinus Tü 365 through the 16S rRNA and 23S rRNA genes, was selected for the study. The fermentation broth extract was partitioned and the bioactive fractions were further fractionated using chromatographic techniques (preparative TLC and HPLC). Through bioautography-guided fractionation, two actinomycins (D and X2) were isolated and identified by spectrometric (ESI-HRMSMS) and spectroscopic (NMR) techniques. The isolated substances were tested in the agar disc diffusion assay (Staphylococcus aureus, Streptococcus pneumoniae, Enterococcus faecalis and Escherichia coli) and by the microdilution method in microplates (Staphylococcus aureus and Mycobacterium smegmatis). Actinomycins D and X2 showed activity against M. smegmatis 16.0 μg/mL for both samples and against S. aureus, 1 μg/mL and 0.5 μg/mL, respectively.

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生物自传法从亚马逊土壤链霉菌Tü肉汤中分离出抗菌放线菌素。

本研究的目的是通过对链霉菌提取物的生物活性引导分析，分离和鉴定其生物活性化合物。从亚马逊土壤中分离的一种链霉菌（Streptomyces）的生物活性提取物，通过16S rRNA和23S rRNA基因鉴定为S. collinus Tü 365。对发酵液进行分离，并采用制备层析（TLC）和高效液相色谱（HPLC）对生物活性组分进行进一步分离。通过生物图谱引导分离，分离得到两种放线菌素（D和X2），并通过光谱（ESI-HRMSMS）和波谱（NMR）技术进行鉴定。采用琼脂盘扩散法（金黄色葡萄球菌、肺炎链球菌、粪肠球菌和大肠杆菌）和微孔板微量稀释法（金黄色葡萄球菌和耻垢分枝杆菌）检测分离物质。放线菌素D和X2对耻垢分枝杆菌的活性分别为16.0 μg/mL和1 μg/mL，对金黄色葡萄球菌的活性分别为0.5 μg/mL。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.