Journal of Microscopy and Ultrastructure最新文献

{"title":"Alpha Thalassemia/Intellectual Disability Syndrome X-Linked Expression Varies Significantly between Androgen Receptor-Positive and Androgen Receptor-Negative Prostatic Adenocarcinoma: Relation to Epidermal Growth Factor Receptor Expression and Clinicopathological Factors.","authors":"Marwa A Abd El-Azeem, Dina A Radi","doi":"10.4103/jmau.jmau_119_22","DOIUrl":"10.4103/jmau.jmau_119_22","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer (PC) is the most common cancer and a leading cause of cancer-related mortality in men worldwide. It has become clear that signaling pathways that are implicated in prostatic carcinoma (PCa) initiation and propagation evolved through interactions of several factors. Alpha Thalassemia/Intellectual Disability syndrome X-linked (ATRX) is a chromatin remodeling protein that has an essential role in telomere stability. The androgen receptor (AR) and growth factors especially the epidermal growth factor receptor (EGFR) seem not to function independently in PCa proliferation. This work aimed to study the expression of ATRX in primary prostatic adenocarcinoma in relation to AR and EGFR expression and the significance of biomarkers expression to the known clinicopathological factors.</p><p><strong>Materials and methods: </strong>Eighty-two primary prostatic adenocarcinoma paraffin blocks were stained immunohistochemically with AR, ATRX, and EGFR polyclonal antibodies. PCa was divided into AR^+/hi and AR^-/lo depending on the percentage and intensity of stained cells regardless of AR heterogeneity. ATRX immunostaining was categorized into ATRX preserved expression or ATRX loss. EGFR expression was grouped into low and high expression according to the staining percentage and intensity.</p><p><strong>Results: </strong>AR^+/hi and preserved ATRX expression significantly were linked to low pT stage, low-grade group, and absence of lymph node invasion. While significant EGFR high expression was related to the high-grade group and the presence of lymph node invasion.</p><p><strong>Conclusion: </strong>ATRX preserved expression varies significantly between AR^+/hi and AR^-/lo PCa which is related to favorable clinicopathological factors. However, the loss of ATRX expression correlated significantly with AR^-/low, high EGFR expression, and adverse clinicopathological factors.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"57-67"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Possible Ameliorating Role of Fisetin on Hepatic Changes Induced by Fluoxetine in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study.","authors":"Dina Fouad El Shaer, Hend Ibrahim Abd El Halim","doi":"10.4103/jmau.jmau_84_22","DOIUrl":"10.4103/jmau.jmau_84_22","url":null,"abstract":"<p><strong>Background: </strong>Fluoxetine (FLX) is one of the selective serotonin reuptake inhibitors, it is widely used to treat neuropsychiatric disorders including depression, but high doses can cause several adverse effects. Fisetin (FIS), a bioactive flavonoid presents in vegetables and fruits, has antioxidant, anti-inflammatory, and anticancer effects.</p><p><strong>Aim: </strong>To evaluate the possible ameliorating effect of FIS on the hepatic alterations induced by FLX in adult male albino rats.</p><p><strong>Materials and methods: </strong>Our study was done, for 3-weeks, on 48 rats that were divided into four groups: Group I (control), Group II received FIS orally (100 mg/kg/day), Group III received FLX orally (10 mg/kg/day), and Group IV concomitantly received FLX and FIS at the same dose and manner of groups II and III. Blood and liver samples were obtained and prepared for histological, immunohistochemical, and biochemical studies.</p><p><strong>Results: </strong>FLX group revealed disturbed liver architecture, hepatocytes with vacuolated cytoplasm, inflammatory cellular infiltration, blood extravasation, and congestion of blood vessels in addition to, a significant increase in the area percentage of caspase-3, inducible nitric oxide synthase and the number of glial fibrillary acidic protein-expressing cells as well as a significant decrease in the area percentage of periodic acid-Schiff stain. Moreover, FLX significantly increased aspartate-aminotransferase and alanine-aminotransferase levels in the serum. In addition, FLX increased malondialdehyde level and decreased superoxide dismutase, glutathione (GSH) peroxidase, and reduced GSH levels in liver tissue. The concomitant administration of FIS ameliorated these alterations.</p><p><strong>Conclusions: </strong>Administration of FIS ameliorated the histological, immunohistochemical, and biochemical alterations induced by FLX in the liver of adult male albino rats.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"161-171"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42117089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Glial Fibrillary Acidic Protein-Immunoreactive Astrocytes in the Prefrontal Cortex of the Male Rat following Chronic Khat Use.","authors":"Paul Bundi Karau, Paul Odula, Moses Obimbo, Amadi Ogonda Ihunwo, Pilani Nkomozepi, Muriira Karau","doi":"10.4103/jmau.jmau_26_22","DOIUrl":"10.4103/jmau.jmau_26_22","url":null,"abstract":"<p><strong>Background: </strong>Long-term khat consumption is associated with significant neurocognitive changes, which have been elucidated in behavioral studies. With current research showing the centrality of astrocytes and other glial cells in neuronal signaling, there is possibility that these cells are also affected by chronic khat use. There is little literature on the structural changes in the prefrontal cortex neuronal and astrocytic cytoarchitecture and morphometry in chronic khat users.</p><p><strong>Objective: </strong>The objective of this study was to describe the changes in astrocyte morphometry and structure in rats after long-term use of khat (miraa).</p><p><strong>Materials and methods: </strong>Adult male Wistar rats, aged 2-3 months, weighing 200-300 g were randomized into four groups of 10 each (control, Group 1, Group 2, and Group 3) to correspond with those used as controls and those that received 500 mg/kg, 1000 mg/kg, and 2000 mg/kg body weight khat extracts, respectively. Fresh khat leaves were purchased from Maua market in Meru, and crude extract was prepared using lyophilization. The control rats were fed on normal diet, while the experimental groups were fed on normal diet and khat extracts using oral gavage for 6 weeks. The animals were sacrificed and their brains were removed. We performed immunohistochemical visualization of astrocytes using glial fibrillary acidic protein. Photomicrographs of the stained sections were transferred to ImageJ Fiji software to study the astrocyte density and astrocytic processes. We used Kruskal-Wallis test to correlate the four animal groups in terms of astrocyte densities.</p><p><strong>Results: </strong>We observed an increase in the average number of astrocytes with increasing doses of khat compared to controls, with those in Group 3 (2000 mg/kg) having an exuberant reactive astrocytosis. Further, escalating khat doses resulted in increased glial fibrillary acidic protein immunoreactivity in the nuclei and astrocytic processes, gliotic changes, and increased complexity of astrocytic processes.</p><p><strong>Conclusion: </strong>Chronic khat use, especially at high doses, results in reactive astrocytosis and astrogliosis, which may be part of the mechanisms involved in the cognitive changes associated with its use.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Adrenal Progenitor Cells in the Structural Response of Adrenal Gland to Various forms of Acute Stress and Subsequent Recovery in Adult Male Albino Rats.","authors":"Doaa Ramadan Sadek, Seham Kamel Abunasef, Sahar Khalil","doi":"10.4103/jmau.jmau_106_22","DOIUrl":"10.4103/jmau.jmau_106_22","url":null,"abstract":"<p><strong>Background: </strong>Stress is a response to stressogenic stimuli that interferes with an organism's homeostasis. The adrenal gland is crucial in the body's reaction to stress.</p><p><strong>Objective: </strong>This study compared the effects of immobility and cold as acute stressors and the subsequent recovery on the histological changes of the adrenal gland and the suspected role of the adrenal progenitor cells.</p><p><strong>Materials and methods: </strong>Thirty-five adult male albino rats were divided equally into five groups. Group I: Control group, Group II: Rats subjected to the acute cold stress procedure, Group III: Rats subjected to the acute immobilization stress procedure, Group IV: The combined stress group, and Group V: Similar to the combined stress group and recovered for 6 days then sacrificed 1 day later. Serum cortisol level was determined, and the adrenal glands were processed for histological and immunohistochemical studies.</p><p><strong>Results: </strong>Serum cortisol concentration was higher in the acute-stress groups and decreased in the recovery group. The adrenal cortex had enlarged, vacuolated cells with pyknotic nuclei, sinusoidal dilatation, and congestion. Chromaffin cells were crowded, enlarged, and vacuolated. There was strong immunohistochemical reactivity for heat shock protein-70 and caspase-3. In addition, the combined group showed a significant increase in the optical density of chromogranin-A in the medullary cells as well as CD44+ve cells. These findings were decreased in the recovery group.</p><p><strong>Conclusions: </strong>The combined stress has more deleterious adrenal cortical changes than immobilization and cold stress alone. The progenitor and chromaffin cells apparently had an important regenerative role in recovery from both types of stress.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"16-24"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Diagnostic Challenge of Sialolithiasis.","authors":"Sahar M N Bukhary","doi":"10.4103/jmau.jmau_92_22","DOIUrl":"10.4103/jmau.jmau_92_22","url":null,"abstract":"<p><p>The major salivary glands (parotid, submandibular, and sublingual) are most frequently obstructed by calculi within the salivary gland, or more uncommonly, by ranulas. Despite the well-defined clinical and radiographic diagnostic features, sialolithiasis may sometimes be confused with sialadenitis and ranulas, especially when encountered in general dental practice. We, therefore, present a case that illustrates this diagnostic dilemma to highlight the salient features of all three conditions. A 28-year-old female presented with a history of a submandibular swelling for 8 months. On intraoral examination, a bluish sublingual swelling was identified at the left side of the lingual frenum, causing a slight elevation of the tongue. The preliminary diagnosis was of a ranula; however, the clinical history suggested sialolithiasis. A hard structure was palpated in the submandibular gland, and a mandibular occlusal film revealed a large ductal sialolith. Sialolithotomy was performed under local anesthesia, and a single 7.2 mm stone was retrieved. The postoperative follow-up period was uneventful, with good healing and restored normal salivary flow. Despite the fairly clear clinical and radiographic diagnostic criteria suggestive of sialolithiasis, the bluish-tinged swelling of the floor of the mouth prompted the examining dentist to provisionally diagnose a ranula. Sialolithiasis is a common obstructive condition of the salivary gland encountered in the dental setting. Despite the clinical and radiographic features usually guiding the correct diagnosis, it can be a challenging diagnosis for less experienced dentists, who must always carefully consider the history, clinical, and radiographic findings.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"221-224"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone Marrow Erythroblastic Dysplasia on Morphology Correlates Significantly with Flow Cytometric Apoptosis and Peripheral Blood Eryptosis.","authors":"Chander Hans, Prashant Sharma, Rahul Saini, M U S Sachdeva, Alka Rani Khadwal, Parveen Lata Bose, Reena Das","doi":"10.4103/jmau.jmau_97_22","DOIUrl":"10.4103/jmau.jmau_97_22","url":null,"abstract":"<p><strong>Introduction: </strong>Erythrocytic damage and death in response to physiochemical, infectious, metabolic, and pharmacological insults have been extensively studied in several diseases. Their relationship with erythroid precursors' apoptosis and morphological dysplasia, however, remains largely unexplored, despite several shared triggers and pathogenetic mechanisms.</p><p><strong>Materials and methods: </strong>We compared peripheral blood phosphatidylserine (PS) exposure and calcium influx in 53 patients with early and late apoptosis of CD71 + ve marrow erythroblasts using flow cytometry. Flow cytometric results were then correlated with dyserythropoiesis in the bone marrow as scored by experienced morphologists.</p><p><strong>Results: </strong>Median patient age was 32 years (range: 1-75 years); 38 (72%) had hemoglobin (Hb) ≤11.0 g%. Patients overall had significantly higher Annexin V binding (PS exposure) and Fluo-3AM signal (calcium influx) vis-à-vis 20 healthy controls. Dyserythropoiesis on morphological evaluation correlated significantly with PS exposure (<i>r</i> = 0.618, <i>P</i> = 0.014) and Fluo-3AM binding (<i>P</i> = 0.002). Patients with dyserythropoiesis had significantly higher apoptosis compared to those without dyserythropoiesis (<i>P</i> = 0.006). In the peripheral blood, Annexin V binding and Fluo-3AM fluorescence correlated strongly with each other (<i>r</i> = 0.885, <i>P</i> < 0.001). PS exposure and Ca²⁺ influx were increased in 64% of cases. These patients had significantly lower Hbs and reticulocyte counts and increased red cell distribution widths and circulating nucleated red blood cell numbers.</p><p><strong>Conclusions: </strong>This is the first study to compare and demonstrate links between dyserythropoiesis, peripheral blood eryptosis, and erythroblastic apoptosis. Eryptosis and apoptosis' interrelationships in patients with diverse hematological disorders link the marrow environment to peripheral blood.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"26 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70802010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hamartoma of the Breast in a Young Female: Radio-Pathological Correlation.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_142_20","DOIUrl":"10.4103/jmau.jmau_142_20","url":null,"abstract":"<p><p>Hamartoma of the breast is a rare benign lesion that leads to unilateral breast enlargement with evidence of a localized palpable mass. Ultrasonography findings are typical and include a well-defined mass lesion of heterogeneous echotexture consisting of mixed echogenic and sonolucent areas. This case report describes a hamartoma of the breast in a 17-year-old female.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"52-53"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendritic Cell-Based Immunity: Screening of Dendritic Cell Subsets in Breast Cancer-Bearing Mice.","authors":"Alia M Aldahlawi, Kawther Sayed Ali Zaher","doi":"10.4103/jmau.jmau_85_22","DOIUrl":"10.4103/jmau.jmau_85_22","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer (BC) is the most devastating disease, particularly the lethal invasive form. It is the most underlying cause of death among women worldwide. The expansion of BC is controlled by a variety of alterations in the tumor cells themselves, in addition to the state of the immune system, which has a direct influence on the tumor microenvironment. Numerous receptors expressed by T-cells interact with ligands on antigen-presenting cells to provide activation signals results in mounting effector anti-tumor T-cell responses. On the other hand, there is a dearth of information about the actual interactions and reactions of T-cells and dendritic cells (DCs) all through the progression of tumor development.</p><p><strong>Aim: </strong>Immune system response against BC was investigated through tumor induction in mice. The size and volume of the tumor were calculated. Moreover, the phenotypical profile of T-cells and DCs from lymph nodes (LN) and spleens of BC-bearing mice was investigated. In addition, the levels of Transforming growth factor-β, Interferon-gamma (IFN-γ), Interleukin IL-2, IL-10, IL-4, IL-12, and tumor necrosis factor (TNF)-α were determined.</p><p><strong>Materials and methods: </strong>MDA231 cells were utilized to induce BC in 30 white BALB/C mice, whereas the other 30 mice acted as healthy controls and were not treated with any cancer-causing agents. The impact of malignancy was evaluated using flow cytometry based on the marking surface molecules, as well as the titer of specific cytokines of the mice's LN culture using the ELISA method. These cytokines included transforming growth factor-β (TGF-β), IFN-γ, IL-2, IL -10, IL -4, IL -12, and TNF-α.</p><p><strong>Results: </strong>The findings showed that the maturation of DCs was inhibited, followed by an accumulation of immature DCs. These immature DCs increase the release of TGF-β and cytokines like IL-10 and inhibit the release of IFN-γ and IL-12 in the culture supernatant of nodal lymph and spleen suspension of BC-bearing mice compared to control. In addition, there was a low expression of CD80 and CD86 on DCs, which indicates a low maturation process.</p><p><strong>Conclusion: </strong>According to the findings, the tumor microenvironment may have been responsible for preventing the maturation of DCs. This, in turn, weakened the immune response and facilitated the ability of the tumor to proliferate. Furthermore, the tumor microenvironment increased the number of immature DCs by inhibiting their stimulation by overexpression of TGF-β-produced by regulatory T lymphocytes and stimulation of tumor cells. In addition, the tumor microenvironment stimulated the secretion of cytokines such as IL-10, and CD4 and decreased the secretion of IFN-γ-and IL-12 in tumor-induced mice cultured LN and spleen.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"150-160"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43371129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of Hematological and Biochemical Parameters as a Screening Tool for Assessing Coronavirus Disease 2019 Infection and its Severity.","authors":"Sana Alam, Sabina Khan, Vineet Jain, Varun Kashyap, Prem Kapur","doi":"10.4103/jmau.jmau_59_22","DOIUrl":"10.4103/jmau.jmau_59_22","url":null,"abstract":"<p><strong>Background: </strong>The rapidly evolving pandemic of Coronavirus disease 2019 (COVID-19) has presented with clinical severity, which varies from asymptomatic cases to being fatal in others. The need of the hour is to find meaningful and cost-effective COVID-19 biomarkers out of conventional hematological and biochemical parameters, which will help in the early identification of patients with a poor prognosis, leading to timely intervention.</p><p><strong>Aim: </strong>The aim was to analyze different biochemical and hematological parameters in COVID-19 patients and also to study the association of these parameters with disease severity.</p><p><strong>Materials and methods: </strong>Cross-sectional observational study was carried out on 100 COVID-19 patients from a hospital from July to October 2020. Based on saturation of oxygen (SpO₂), admitted patients were grouped into mild-moderate (SpO₂ ≥90%) and severe groups (SpO₂ <90%). Hematological and biochemical parameters were studied in both groups, and association with disease severity was analyzed.</p><p><strong>Results: </strong>Out of 100 patients, 57 patients were seen in the mild-moderate group (SpO₂ ≥90%), while 43 patients (SpO₂ <90%) belonged to the severe category. Males were predominant in both mild-moderate and severe groups. Among the hematological parameters, statistically significant higher values of absolute neutrophil count (<i>P</i> = 0.046) and significantly lower absolute lymphocyte count (<i>P</i> = 0.003) values were observed. With regard to biochemical parameters, increased urea and decreased total protein were found in the severe category and this association was statistically significant.</p><p><strong>Conclusion: </strong>To conclude, early identification and monitoring of hematological and biochemical parameters, especially those associated with higher disease severity, may contribute toward improving disease outcomes.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"214-220"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concomitant Hodgkin's Lymphoma in a Patient with Tuberculous Spondylitis.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_128_20","DOIUrl":"10.4103/jmau.jmau_128_20","url":null,"abstract":"<p><p>Concomitant Hodgkin's lymphoma with tuberculosis is an exceedingly rare clinical scenario and a condition that is difficult to manage due to similar clinical presentation. This case report describes the same in a 44-year-old male patient diagnosed with Koch's and initiated on antituberculosis therapy, based on confirmation of findings from the spine biopsy and culture. The patient's clinical condition worsened despite being on treatment for tuberculosis. Hence, further work up of the patient was done which included mediastinoscopy and endobronchial ultrasound. Biopsy samples from a conglomerate mass in the lower cervical region and mediastinum revealed Hodgkin's lymphoma of the nodular sclerosis type. This time, the patient showed significant improvement following treatment with chemotherapy and radiotherapy along with antituberculosis therapy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"242-244"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10779446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}