Journal of Microscopy and Ultrastructure最新文献

{"title":"Bone Marrow Erythroblastic Dysplasia on Morphology Correlates Significantly with Flow Cytometric Apoptosis and Peripheral Blood Eryptosis.","authors":"Chander Hans, Prashant Sharma, Rahul Saini, M U S Sachdeva, Alka Rani Khadwal, Parveen Lata Bose, Reena Das","doi":"10.4103/jmau.jmau_97_22","DOIUrl":"10.4103/jmau.jmau_97_22","url":null,"abstract":"<p><strong>Introduction: </strong>Erythrocytic damage and death in response to physiochemical, infectious, metabolic, and pharmacological insults have been extensively studied in several diseases. Their relationship with erythroid precursors' apoptosis and morphological dysplasia, however, remains largely unexplored, despite several shared triggers and pathogenetic mechanisms.</p><p><strong>Materials and methods: </strong>We compared peripheral blood phosphatidylserine (PS) exposure and calcium influx in 53 patients with early and late apoptosis of CD71 + ve marrow erythroblasts using flow cytometry. Flow cytometric results were then correlated with dyserythropoiesis in the bone marrow as scored by experienced morphologists.</p><p><strong>Results: </strong>Median patient age was 32 years (range: 1-75 years); 38 (72%) had hemoglobin (Hb) ≤11.0 g%. Patients overall had significantly higher Annexin V binding (PS exposure) and Fluo-3AM signal (calcium influx) vis-à-vis 20 healthy controls. Dyserythropoiesis on morphological evaluation correlated significantly with PS exposure (<i>r</i> = 0.618, <i>P</i> = 0.014) and Fluo-3AM binding (<i>P</i> = 0.002). Patients with dyserythropoiesis had significantly higher apoptosis compared to those without dyserythropoiesis (<i>P</i> = 0.006). In the peripheral blood, Annexin V binding and Fluo-3AM fluorescence correlated strongly with each other (<i>r</i> = 0.885, <i>P</i> < 0.001). PS exposure and Ca²⁺ influx were increased in 64% of cases. These patients had significantly lower Hbs and reticulocyte counts and increased red cell distribution widths and circulating nucleated red blood cell numbers.</p><p><strong>Conclusions: </strong>This is the first study to compare and demonstrate links between dyserythropoiesis, peripheral blood eryptosis, and erythroblastic apoptosis. Eryptosis and apoptosis' interrelationships in patients with diverse hematological disorders link the marrow environment to peripheral blood.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"26 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063927/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70802010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hamartoma of the Breast in a Young Female: Radio-Pathological Correlation.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_142_20","DOIUrl":"10.4103/jmau.jmau_142_20","url":null,"abstract":"<p><p>Hamartoma of the breast is a rare benign lesion that leads to unilateral breast enlargement with evidence of a localized palpable mass. Ultrasonography findings are typical and include a well-defined mass lesion of heterogeneous echotexture consisting of mixed echogenic and sonolucent areas. This case report describes a hamartoma of the breast in a 17-year-old female.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"52-53"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063920/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dendritic Cell-Based Immunity: Screening of Dendritic Cell Subsets in Breast Cancer-Bearing Mice.","authors":"Alia M Aldahlawi, Kawther Sayed Ali Zaher","doi":"10.4103/jmau.jmau_85_22","DOIUrl":"10.4103/jmau.jmau_85_22","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer (BC) is the most devastating disease, particularly the lethal invasive form. It is the most underlying cause of death among women worldwide. The expansion of BC is controlled by a variety of alterations in the tumor cells themselves, in addition to the state of the immune system, which has a direct influence on the tumor microenvironment. Numerous receptors expressed by T-cells interact with ligands on antigen-presenting cells to provide activation signals results in mounting effector anti-tumor T-cell responses. On the other hand, there is a dearth of information about the actual interactions and reactions of T-cells and dendritic cells (DCs) all through the progression of tumor development.</p><p><strong>Aim: </strong>Immune system response against BC was investigated through tumor induction in mice. The size and volume of the tumor were calculated. Moreover, the phenotypical profile of T-cells and DCs from lymph nodes (LN) and spleens of BC-bearing mice was investigated. In addition, the levels of Transforming growth factor-β, Interferon-gamma (IFN-γ), Interleukin IL-2, IL-10, IL-4, IL-12, and tumor necrosis factor (TNF)-α were determined.</p><p><strong>Materials and methods: </strong>MDA231 cells were utilized to induce BC in 30 white BALB/C mice, whereas the other 30 mice acted as healthy controls and were not treated with any cancer-causing agents. The impact of malignancy was evaluated using flow cytometry based on the marking surface molecules, as well as the titer of specific cytokines of the mice's LN culture using the ELISA method. These cytokines included transforming growth factor-β (TGF-β), IFN-γ, IL-2, IL -10, IL -4, IL -12, and TNF-α.</p><p><strong>Results: </strong>The findings showed that the maturation of DCs was inhibited, followed by an accumulation of immature DCs. These immature DCs increase the release of TGF-β and cytokines like IL-10 and inhibit the release of IFN-γ and IL-12 in the culture supernatant of nodal lymph and spleen suspension of BC-bearing mice compared to control. In addition, there was a low expression of CD80 and CD86 on DCs, which indicates a low maturation process.</p><p><strong>Conclusion: </strong>According to the findings, the tumor microenvironment may have been responsible for preventing the maturation of DCs. This, in turn, weakened the immune response and facilitated the ability of the tumor to proliferate. Furthermore, the tumor microenvironment increased the number of immature DCs by inhibiting their stimulation by overexpression of TGF-β-produced by regulatory T lymphocytes and stimulation of tumor cells. In addition, the tumor microenvironment stimulated the secretion of cytokines such as IL-10, and CD4 and decreased the secretion of IFN-γ-and IL-12 in tumor-induced mice cultured LN and spleen.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"150-160"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679829/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43371129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utility of Hematological and Biochemical Parameters as a Screening Tool for Assessing Coronavirus Disease 2019 Infection and its Severity.","authors":"Sana Alam, Sabina Khan, Vineet Jain, Varun Kashyap, Prem Kapur","doi":"10.4103/jmau.jmau_59_22","DOIUrl":"10.4103/jmau.jmau_59_22","url":null,"abstract":"<p><strong>Background: </strong>The rapidly evolving pandemic of Coronavirus disease 2019 (COVID-19) has presented with clinical severity, which varies from asymptomatic cases to being fatal in others. The need of the hour is to find meaningful and cost-effective COVID-19 biomarkers out of conventional hematological and biochemical parameters, which will help in the early identification of patients with a poor prognosis, leading to timely intervention.</p><p><strong>Aim: </strong>The aim was to analyze different biochemical and hematological parameters in COVID-19 patients and also to study the association of these parameters with disease severity.</p><p><strong>Materials and methods: </strong>Cross-sectional observational study was carried out on 100 COVID-19 patients from a hospital from July to October 2020. Based on saturation of oxygen (SpO₂), admitted patients were grouped into mild-moderate (SpO₂ ≥90%) and severe groups (SpO₂ <90%). Hematological and biochemical parameters were studied in both groups, and association with disease severity was analyzed.</p><p><strong>Results: </strong>Out of 100 patients, 57 patients were seen in the mild-moderate group (SpO₂ ≥90%), while 43 patients (SpO₂ <90%) belonged to the severe category. Males were predominant in both mild-moderate and severe groups. Among the hematological parameters, statistically significant higher values of absolute neutrophil count (<i>P</i> = 0.046) and significantly lower absolute lymphocyte count (<i>P</i> = 0.003) values were observed. With regard to biochemical parameters, increased urea and decreased total protein were found in the severe category and this association was statistically significant.</p><p><strong>Conclusion: </strong>To conclude, early identification and monitoring of hematological and biochemical parameters, especially those associated with higher disease severity, may contribute toward improving disease outcomes.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"214-220"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concomitant Hodgkin's Lymphoma in a Patient with Tuberculous Spondylitis.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_128_20","DOIUrl":"10.4103/jmau.jmau_128_20","url":null,"abstract":"<p><p>Concomitant Hodgkin's lymphoma with tuberculosis is an exceedingly rare clinical scenario and a condition that is difficult to manage due to similar clinical presentation. This case report describes the same in a 44-year-old male patient diagnosed with Koch's and initiated on antituberculosis therapy, based on confirmation of findings from the spine biopsy and culture. The patient's clinical condition worsened despite being on treatment for tuberculosis. Hence, further work up of the patient was done which included mediastinoscopy and endobronchial ultrasound. Biopsy samples from a conglomerate mass in the lower cervical region and mediastinum revealed Hodgkin's lymphoma of the nodular sclerosis type. This time, the patient showed significant improvement following treatment with chemotherapy and radiotherapy along with antituberculosis therapy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"242-244"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10779446/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rare and Unusual Occurrence of Splenic Hamartoma on Ultrasonography.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_134_20","DOIUrl":"10.4103/jmau.jmau_134_20","url":null,"abstract":"<p><p>Vascular tumors constitute the most common primary tumors of the spleen. Splenic hamartoma and littoral cell angioma have been reported only in the spleen. Splenic hamartomas are rare benign vascular tumors which are incidentally detected during imaging and are seldom symptomatic. This case report describes a rare sonological appearance of splenic hamartoma in a 31-year-old female with occasional pain in the left hypochondrium.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"54-56"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Giant Placental Chorioangioma in a Young Patient Causing Adverse Fetal Outcome.","authors":"Shagun Wadhwa, Sonali Dixit, Sonal Sharma","doi":"10.4103/jmau.jmau_71_22","DOIUrl":"10.4103/jmau.jmau_71_22","url":null,"abstract":"<p><p>Chorioangiomas are benign vascular tumors of the placenta originating from chorionic tissue. They are also known as hemangiomas of the placenta. They occur in approximately 0.5%-1% of all pregnancies. Large chorioangiomas are rare and may lead to serious fetal and maternal complications. Here, we are describing a case of giant placental chorioangioma in a 19-year-old young female (G2A1) who presented to us at 39 weeks of gestation with decreased fetal movements. Ultrasound examination revealed an enlarged placenta with a well-defined 9.4 cm × 9.3 cm heteroechoic area with increased vascularity. Cesarean section was performed in view of fetal distress and a female baby weighing 1.6 kg was delivered. The newborn died within 2 weeks due to pulmonary hypoplasia and hemodynamic failure. The diagnosis of chorioangioma was confirmed with histopathology. This case depicts the necessity of early diagnosis, close fetal monitoring, and timely intervention in achieving a favorable pregnancy outcome.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"225-227"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729019/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell of Origin Classification of Diffuse Large B-Cell Lymphoma.","authors":"Lity Dhar, Sarika Singh, Shyam Lata Jain, Anubhav Vindal, Pallavi Sinha, Rashmi Gautam","doi":"10.4103/jmau.jmau_66_22","DOIUrl":"10.4103/jmau.jmau_66_22","url":null,"abstract":"<p><strong>Context: </strong>Diffuse large B-cell lymphoma (DLBCL) is a neoplasm of medium-to-large B lymphoid cells with diffuse growth patterns. Although it is a potentially curable disease, around 40% of the cases are either refractory to primary treatment or relapse. Based on gene expression profiling (GEP), DLBCL can be classified as germinal center B-cell subtype (GCB) and activated B-cell subtype (ABC). About 10%-15% of cases do not convincingly fall into either of the two subtypes and hence remain unclassified. Most widely used and suggested by WHO is Hans algorithm comprising immunohistochemical markers CD10, B-cell lymphoma6 (BCL6), and IRF4/MUM1, which classifies CD10+ and CD10-/BCL6+/MUM1-DLBCL as GCB, while CD10-/BCL6+/MUM1 + and BCL6-DLBCL as non-GCB.</p><p><strong>Aims: </strong>The aim of this study was to classify DLBCL into GCB and non-GCB subtypes using Hans Algorithm.</p><p><strong>Settings and design: </strong>This was a retrospective study.</p><p><strong>Materials and methods: </strong>Twenty-eight histologically diagnosed cases of nodal (71.4%), as well as extranodal (28.6%) DLBCL, were taken over the period of 2 years with age ranging between 10 and 65 years with 19 males and 9 females. M: F = 2.1:1. Depending upon the site involved, a primary panel of immunohistochemistry (IHC) markers, namely CD20, CD3, LCA, EMA, and CK, followed by a secondary panel comprising CD10, CD19, CD30, LMP1, BCL2, BCL6, MUM1, MYC, and FOXP1 was used.</p><p><strong>Results: </strong>In this study, it was found that the non-GCB subtype was more common than the GCB subtype in Indian population.</p><p><strong>Conclusions: </strong>Although the gold standard of GEP to assign cells of origin is using RNA microarray analysis, however, due to resource constraints and other limitations such as long turnaround times, IHC is the next acceptable alternative.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"193-198"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid Diagnosis and Epidemiology of Fungal Rhinosinusitis in PostCOVID-19 Patients.","authors":"Mayuri K Bapodra, Anamika J Navadiya, Seema N Baxi, Mayuri Rajendra Gohil, Pankita K Parmar","doi":"10.4103/jmau.jmau_63_21","DOIUrl":"10.4103/jmau.jmau_63_21","url":null,"abstract":"<p><strong>Background: </strong>Fungal rhinosinusitis (FRS) cases are not exiguous for the world. However, their spike as a postCOVID sequelae has alarmed the world, especially India. Adding to the woes is the high mortality rate and poor prognosis associated with acute invasive fungal rhinosinusitis (AIFR) in such immunocompromised patients. In such a scenario, early and precise diagnosis of AIFR is what a patient and physician banks upon. KOH and histopathology are the two frontline investigations for the diagnosis of FRS. Our study aimed at analyzing the cases of FRS by histopathology and comparing these with KOH.</p><p><strong>Study design and materials and methods: </strong>Prospective longitudinal study including suspected postCOVID FRS patients over a period of 1.5 months. Their clinical, histopathological, and KOH findings were then correlated.</p><p><strong>Results: </strong>About 72.5% clinically suspected fungal infection specimens were found to be positive for fungal elements on histopathology. Of these, only 30 cases were positive by KOH mount. Maximum patients belonged to 40-70 years of age; males (67%) more than females. Sites involved were paranasal sinuses (100%), nasal (88%), and orbital (25%). Histopathology revealed mucormycosis (100%) and aspergillosis (16%). Angioinvasion was identified in 38% of the mucormycosis cases.</p><p><strong>Conclusion: </strong>In a state of crisis, when the gold standard for fungal identification i.e., culture can take as many as 21 days for final report and early and judicious antifungal treatment is sine qua non of recovery, histopathology has proved to be better than KOH as far as early and precise diagnosis of fungal elements and their invasion is concerned.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"237-241"},"PeriodicalIF":0.0,"publicationDate":"2023-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10779442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Possible Ameliorative Effect of <i>Echinacea</i>, Ginger, and Their Combination on Experimentally Induced Diabetic Nephropathy in a Rat Model: Histological and Immunohistochemical Study.","authors":"Hoda M Elsayed, Hekmat Osman Abdel-Aziz, Ghada Mohammed Ahmed, Mohamed Arafa Adly, Sherine Ahmed Mohammed","doi":"10.4103/jmau.jmau_62_22","DOIUrl":"10.4103/jmau.jmau_62_22","url":null,"abstract":"<p><strong>Background: </strong>Diabetes represents a chronic disease characterized by hyperglycemia. Several changes in the renal functions had been detected in diabetic patients.</p><p><strong>Aim of the work: </strong>This study was conducted to compare the possible ameliorative role of both ginger and <i>Echinacea</i> either alone or in combination upon experimentally induced diabetic nephropathy.</p><p><strong>Materials and methods: </strong>Sixty adult male albino rats were used in this study. Rats were divided into three groups. Control (group I) included 20 rats. Diabetic group (group II) included 10 rats. Group III included 30 rats subdivided into three subgroups 10 animals each: Subgroup IIIa diabetic treated with 100 mg/kg <i>Echinacea</i> for 30 days. Subgroup IIIb diabetic treated with 400 mg/kg ginger for 30 days orally. Subgroup IIIc diabetic treated with both 100 mg/kg <i>Echinacea</i> and 400 mg/kg ginger for 30 days orally. Hemotoxylin and eosin staining, Periodic acid Schiff and Masson trichrome were done. Ultrastructural examination was done. Immunohistochemical markers used were caspase-3 for apoptosis and CD68 for macrophages. Morphometric and statistical analyses were done.</p><p><strong>Results: </strong>Diabetes caused a significant increase in collagen fibers in the renal cortex, the caspase-3 expression as well as the number of macrophages. Ultrastructurally, there was an irregularly thickened glomerular basement membrane and effacement of podocytes. Ginger treatment alone or in combination with <i>Echinacea</i> exhibited more pronounced improvement of diabetes-induced degenerative changes and a significant decrease in collagen fibers, the caspase-3 expression as well as the number of macrophages compared to <i>Echinacea</i> alone.</p><p><strong>Conclusion: </strong>Ginger treatment alone or in combination with <i>Echinacea</i> exhibited more pronounced improvement in diabetes nephropathy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"199-210"},"PeriodicalIF":0.0,"publicationDate":"2023-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729022/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}