Journal of Microscopy and Ultrastructure最新文献

{"title":"Preliminary Report on Histopathology of Odontogenic Keratocyst in Third Dimension: Three-Dimensional Video and Images.","authors":"Sandhya Tamgadge,&nbsp;Avinash Tamgadge","doi":"10.4103/jmau.jmau_1_21","DOIUrl":"https://doi.org/10.4103/jmau.jmau_1_21","url":null,"abstract":"<p><strong>Introduction: </strong>Odontogenic keratocyst (OKC) is a developmental odontogenic cyst of the jaws that causes extensive destruction even before the clinical manifestations are evident. It is a well-known entity among private practitioners as it has a high recurrence rate and requires meticulous treatment planning. Unfortunately, clinicians have less understood its basic histopathology.</p><p><strong>Objective: </strong>This article highlights the preliminary attempt of an author in designing third dimension of histopathological aspect of OKC so that it is easily understood by oral pathologists, general pathologists, nonpathologists, students, and even patients too.</p><p><strong>Methodology: </strong>Preliminary three-dimensional (3D) images and videos on histopathological aspect of OKC were designed by the author using 3DMax (Autodesk Media and Entertainment) and Adobe Premiere Pro 5.5 software which is a video editing software (Adobe Systems) based on two-dimensional descriptions mentioned in the literature.</p><p><strong>Results: </strong>Internal cellular architecture of OKC was created in a lifelike manner through 3D animation video and images.</p><p><strong>Conclusion: </strong>Histopathological aspect of all oral lesions should be explained in third dimension too.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"121-123"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5e/a4/JMAU-11-121.PMC10337673.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of p53 as Potential Biomarker in Oral Submucous Fibrosis: An Immunohistochemical Study.","authors":"K A Kamala,&nbsp;Sujata R Kanetkar,&nbsp;Kailash D Datkhile,&nbsp;S Sankethguddad","doi":"10.4103/jmau.jmau_23_21","DOIUrl":"https://doi.org/10.4103/jmau.jmau_23_21","url":null,"abstract":"<p><strong>Background: </strong>Oral submucous fibrosis (OSMF) is potentially malignant disorder known to transform into oral cancer. The malignant transformation is often associated with changes at the genetic level that in turn is reflected by the altered expression of proteins related to cell cycle, proliferation, and apoptosis. Expression of p53 tumor suppressor gene is one of the common findings in human cancers including the oral cancer. Therefore, the early detection of potentially malignant OSMF has been crucial in the inhibition of oral cancer.</p><p><strong>Aim and objectives: </strong>To determine the main pathological logical factors and expression of aberrant p53 in OSMF, oral squamous cell carcinomas (OSCC) and in normal patients, to study correlation between p53 expression with clinical staging and histological grading of OSMF.</p><p><strong>Materials and methods: </strong>An immunohistochemical (IHC) study was performed for p53 expression on 35 cases of OSMF, 10 cases of OSCC with history of habits and 10 normal patients without any habits.</p><p><strong>Results: </strong>The expression of p53 showed a significant difference between normal oral mucosa, OSMF and OSCC samples.</p><p><strong>Conclusion: </strong>The study demonstrated a high incidence of p53 over expression in OSMF and OSCC. The results indicate that p53 over expression may play a role in pathogenesis of OSMF and in the development of Oral squamous cell carcinoma. With early detection of the high-risk patients with OSMF, we can expect to develop more intensive treatment modalities, leading to the reduction in cancer transformation rate from OSMF.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/9d/JMAU-11-75.PMC10337671.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light Microscopy and Ultrastructure of Body Wall in Leech <i>Haemadipsa zeylanica</i>.","authors":"Usha Anilkumar,&nbsp;Anita S Jadhav","doi":"10.4103/jmau.jmau_45_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_45_22","url":null,"abstract":"<p><strong>Background information: </strong>Leeches are widely distributed worldwide in a diversity of habitats, such as freshwater, seas, desert, and oases. Since literature does not have an elaborate study on histology of leeches, thus emphasis is given to study the histology.</p><p><strong>Aim: </strong>The aim of this study is to examine the histology of free-living leeches <i>Haemadipsa</i> <i>zeylanica</i> under light microscopy and extended to ultrastructural study under transmission electron microscopy.</p><p><strong>Materials and methods: </strong>Tissue was fixed in 10% formalin, dehydrated with alcohol, infiltrated and embedded in wax, and thin section was cut using a rotary microtome. Sections were stained with hematoxylin and eosin.</p><p><strong>Results: </strong>The epithelial layer of cells in <i>H. zeylanica</i> had a thin layer of cuticles covering it. In light microscopy, the epidermis has three types of epidermal cells, namely, glandular cells (Type I, Type II, and Type III), supporting cells (Type IV), and pigment cells (Type V). When the three glandular cells of the body wall of <i>H. zeylanica</i> epidermis were examined under an electron microscope, three types of distinct electron-dense granules were identified. Type I cells with granules, Type II cells with course granules close to the dermis were identified as pear-shaped secretory cells, and Type III cells with electron-dense granules.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"81-86"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/f8/JMAU-11-81.PMC10337674.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placental Dysfunction and Acute Toxoplasmosis: The Role of Melatonin in Relation to Inflammatory Cytokines Interleukin-10 and Interleukin-12.","authors":"Azhar H Al-Kuraishi,&nbsp;Hatham Ibraheem Khalil,&nbsp;Huda Hameed Hassan,&nbsp;Hayder M Al-Kuraishy","doi":"10.4103/jmau.jmau_122_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_122_20","url":null,"abstract":"Objective: The objective of this study was to elucidate the potential role of anti-inflammatory interleukin (IL)-10 and pro-inflammatory (IL-12) cytokines as well as melatonin (MEL) in the development of placental dysfunction in the pregnant women with acquired toxoplasmosis (TOX). Materials and Methods: This case–control study was carried out at the Department of Clinical Parasitology in teamwork with the Department of Gynecology and Obstetrics, Al-Yarmouk Teaching Hospital, College of Medicine, Al-Mustansiriyah University, Baghdad, Iraq, from September 2018 to February 2019. The recruited patients and healthy controls were allocated into two groups – Group A: pregnant women with acute TOX (n = 45) and Group B: healthy pregnant women (n = 25). Anti-Toxoplasma (Toxo) immunoglobulin M (IgM), serum and placental as well as serum–placental (SP) ratio of MEL, IL-10, and IL-12 were measured. SPSS version 20.00 was used for data analysis. Results: Anti-Toxo IgM serum level and IL-12 serum levels were higher compared with controls (P = 0.001). Both MEL and IL-10 serum levels were lower in the pregnant women with acute TOX compared with controls (P = 0.002 and P = 0.002), respectively. Besides, Both MEL and IL-10 placental levels were lower in the pregnant women with acute TOX compared with controls (P = 0.001). As well, placental IL-12 level was higher in the pregnant women with acute TOX compared with controls (P = 0.001). SP ratio of MEL was higher in the pregnant women with acute TOX compared with controls (P = 0.001). Conclusion: MEL and anti-inflammatory IL-10 are reduced in the pregnant women with acute TOX, while IL-12 is increased. SP ratio of MEL but not of IL-10 or IL-12 is elevated in the pregnant women with acute TOX reflecting the risk of PD.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"87-91"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/c0/JMAU-11-87.PMC10337677.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological, Clinical, and Histopathological Features of Salivary Gland Tumors among 150 Sudanese Patients: 10 Years' Experience.","authors":"Somaya T S Hamid,&nbsp;Mohammed A M Abdalla,&nbsp;Azza A Zulfu,&nbsp;Azza Ramadan,&nbsp;Musaab Ahmed,&nbsp;Mohamed H Ahmed","doi":"10.4103/jmau.jmau_113_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_113_20","url":null,"abstract":"<p><strong>Background and objectives: </strong>Salivary gland tumors (SGTs) are serious challenges to pathologists. Herein, we aimed to assess epidemiological and histopathological characteristics of SGTs among Sudanese patients.</p><p><strong>Materials and methods: </strong>This retrospective descriptive study was undertaken at The pathology department in Khartoum State between 2008 and 2018. Patient records, histopathological reports, and slides were retrieved; and re-examined by two histopathologists. Diagnoses were reclassified according to the 2017 WHO classification of SGTs.</p><p><strong>Results: </strong>Overall, 150 cases of Sudanese patients with SGT were included (90 [60%] males and 60 [40%] females). Among these, 105 were benign (70%) and 45 were malignant (30%). The parotid glands were the most common site for both benign and malignant tumors (77/150; 51%: 59 benign (76.6%) and 18 malignant [23.4%]). The next common site was the submandibular gland (54 [36%]: 38 benign [70.3%] and 16 malignant [29.7%]), followed by minor salivary glands (19 [12.7%]: 8 benign and 11 malignant [57.9%]). Benign gland entities included pleomorphic adenoma (88/105; 83.7%), oncocytoma (5/105; 4.8%), myoepithelioma (4/105; 3.8%), Whartin tumors (3/105; 2.9%), basal cell adenoma (3/105; 2.9%), and sialolipoma (2/105; 1.9%). Malignant gland entities included adenoid cystic carcinoma (12; 26.7%), mucoepidermoid carcinoma (10; 22,2%), acinic cell carcinoma (6; 13.3%), poorly differentiated carcinoma (4; 8.9%), adenocarcinoma NOS (not otherwise specified) (4; 8.9%), basal cell adenocarcinoma (3; 6.7%), carcinoma ex pleomorphic adenoma (3; 6.7%), polymorphous adenocarcinoma (2; 4.4%), salivary duct carcinoma (1; 2.2%), and epithelial-myoepithelial carcinoma (2.2%).</p><p><strong>Conclusions: </strong>SGTs shared several epidemiological and histopathological features, exhibiting high incidence in the parotid and submandibular glands, lower prevalence in minor glands, and greater male predominance.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"92-96"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/3d/JMAU-11-92.PMC10337678.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Correlation of Serum Histamine and Mast Cell Count with the Established Prognosticators in Oral Cancer.","authors":"Nabeel Rashid Hasan,&nbsp;Priyadarshini Dehuri,&nbsp;Amitabh Jena,&nbsp;Srikanta Kanungo","doi":"10.4103/jmau.jmau_138_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_138_20","url":null,"abstract":"<p><strong>Context: </strong>We undertook this study in view of the rising interest in the interactions between tumor cells and mast cells in the tumor microenvironment and the mounting evidence of the role of mast cells in the prognosis of various cancers.</p><p><strong>Aims: </strong>The aim of the study is to determine the prognostic utility of mast cells and their mediators in oral squamous cell carcinoma (OSCC).</p><p><strong>Settings and design: </strong>This is a cross-sectional analytical study. A total of 41 cases were studied for determining the correlation of mast cell counts with histopathological prognostic variables and 29 cases for determining the serum histamine levels and to further correlate the values with the histopathologic determinants.</p><p><strong>Subjects and methods: </strong>Mast cell assessment was done using Toluidine blue stain on tissue sections while histopathological prognostic factors were evaluated with routine stains. The serum histamine levels were obtained by sandwich enzyme linked immunosorbent assay (ELISA).</p><p><strong>Statistical analysis: </strong>Mann-Whitney <i>U</i>-test was used to test the difference between parameters and a Spearman correlation coefficient was used. <i>P</i> < 0.05 was considered significant for statistical analysis.</p><p><strong>Results: </strong>We found statistically significant correlations between increased median mast cell counts and higher grade of tumor, presence of lymphovascular invasion, greater depth of invasion, and presence of regional lymph node involvement. The serum histamine values correlated only with the depth of tumor invasion.</p><p><strong>Conclusions: </strong>Hence, as per our results, we suggest the use of mast cell counts in OSCC cases as a prognostic indicator in conjunction with other clinicopathological factors. At the same time, we acknowledge the fact that extensive studies are required to establish the role of mast cell mediators in the prognostic curve of oral cancer patients.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"97-102"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/02/JMAU-11-97.PMC10337676.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.","authors":"Heba A Mahmoud,&nbsp;Hemat E El Horany,&nbsp;Marwa Aboalsoud,&nbsp;Rania Nagi Abd-Ellatif,&nbsp;Amal Ahmed El Sheikh,&nbsp;Alshimaa Aboalsoud","doi":"10.4103/jmau.jmau_78_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_78_22","url":null,"abstract":"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Presentation of Multifocal Anaplastic Oligodendroglioma.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_126_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_126_20","url":null,"abstract":"<p><p>Multifocal tumors are usually reported within the same cerebral hemisphere due to widespread dissemination along the white matter tracts. This case report describes the magnetic resonance imaging appearances of multifocal anaplastic oligodendroglioma in a 28-year-old adult male that showed three discrete heterogeneously enhancing cortical-based lesions in the left frontoparietal lobes. Left frontal craniotomy was performed and biopsy of the lesion was obtained, histopathology of which showed features of anaplastic oligodendroglioma.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"115-117"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e2/60/JMAU-11-115.PMC10337670.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pleomorphic Adenoma in an Immunocompromised Patient.","authors":"Cathy Babu, Sandhya Tamgadge, Treville Pereira, Vishal Punjabi","doi":"10.4103/jmau.jmau_90_22","DOIUrl":"10.4103/jmau.jmau_90_22","url":null,"abstract":"<p><p>Salivary gland tumors are known for its rarity and almost 30%-40% occur in the minor salivary glands, out of which, pleomorphic adenoma (PA) is rated to be the most common benign salivary gland neoplasm. This term was first termed by Willis and is called so, because of the ability of the cells of this tumor to differentiate into epithelial cells and mesenchymal cells, which gives it an unusual histologic pattern. Literature reports most PAs to be associated with parotid salivary glands and palatal salivary glands, the latter being the most common intraoral site of origin. Interestingly, no case reports have been published of a palatal PA in an immunocompromised individual. We report a unique case of a PA arising on the hard palate of a 54-year-old male human immunodeficiency virus-acquired immunodeficiency syndrome patient.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"162-165"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold Nanoparticles Down-Regulate Alpha Fetoprotein Expression Induced by Meloxicam Hepatotoxicity in Adult Male Albino Rats: Histological and Immunohistochemical Study.","authors":"Hekmat Osman Abdel Aziz, Mai Nashat, Aziz Awaad, Sherine Ahmed Mohammed","doi":"10.4103/jmau.jmau_109_22","DOIUrl":"10.4103/jmau.jmau_109_22","url":null,"abstract":"<p><strong>Background: </strong>Meloxicam is a non-steroidal anti-inflammatory drug most commonly used for the treatment of arthritis. Meloxicam decreases prostaglandin E2 resulting in an increase in free radical concentration within the cell. Alpha-fetoprotein (AFP) is a protein produced normally by the fetal liver in hepatoblasts. In inflammatory conditions, the adult liver synthesizes AFP by regenerating cells. Gold nanoparticles (AuNPs) in the medical field, represent one of the most commonly studied metal nanoparticles which have antioxidant properties.</p><p><strong>Objective: </strong>This study was conducted to evaluate the possible therapeutic effects of AuNPs on Meloxicam induced degenerative changes in rat liver.</p><p><strong>Materials and methods: </strong>fifty adult male albino rats were divided into 8 groups: The first group (control); the AuNPs group was treated with AuNPs daily for 2 weeks. The MEL 2w& MEL 2m groups were treated with meloxicam daily for 2 weeks and 2 months respectively. The MEL2w+AuNPs & MEL2m+AuNPs groups received AuNPs for 2 weeks after meloxicam injection daily for 2 weeks and 2 months respectively. The MEL2w+SAL & MEL2m+SAL groups were given meloxicam for 2 weeks and 2 months respectively followed by saline injection for 2 weeks. Histological changes, AuNPs localization in the liver by silver nitrate stain, and AFP immunoexpression were studied.</p><p><strong>Results: </strong>Time dependent Degenerative changes and increased AFP expression were observed in the liver after meloxicam injection. However, AuNPs ameliorated these changes and decreased AFP expression. AuNPs were detected in Kupffer cells.</p><p><strong>Conclusion: </strong>AuNPs could ameliorate meloxicam-induced toxicity in the liver and decrease AFP expression because AuNPs act as free radical scavengers which accumulate in Kupffer cells.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"114 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}