Journal of Microscopy and Ultrastructure最新文献

{"title":"Placental Dysfunction and Acute Toxoplasmosis: The Role of Melatonin in Relation to Inflammatory Cytokines Interleukin-10 and Interleukin-12.","authors":"Azhar H Al-Kuraishi, Hatham Ibraheem Khalil, Huda Hameed Hassan, Hayder M Al-Kuraishy","doi":"10.4103/jmau.jmau_122_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_122_20","url":null,"abstract":"Objective: The objective of this study was to elucidate the potential role of anti-inflammatory interleukin (IL)-10 and pro-inflammatory (IL-12) cytokines as well as melatonin (MEL) in the development of placental dysfunction in the pregnant women with acquired toxoplasmosis (TOX). Materials and Methods: This case–control study was carried out at the Department of Clinical Parasitology in teamwork with the Department of Gynecology and Obstetrics, Al-Yarmouk Teaching Hospital, College of Medicine, Al-Mustansiriyah University, Baghdad, Iraq, from September 2018 to February 2019. The recruited patients and healthy controls were allocated into two groups – Group A: pregnant women with acute TOX (n = 45) and Group B: healthy pregnant women (n = 25). Anti-Toxoplasma (Toxo) immunoglobulin M (IgM), serum and placental as well as serum–placental (SP) ratio of MEL, IL-10, and IL-12 were measured. SPSS version 20.00 was used for data analysis. Results: Anti-Toxo IgM serum level and IL-12 serum levels were higher compared with controls (P = 0.001). Both MEL and IL-10 serum levels were lower in the pregnant women with acute TOX compared with controls (P = 0.002 and P = 0.002), respectively. Besides, Both MEL and IL-10 placental levels were lower in the pregnant women with acute TOX compared with controls (P = 0.001). As well, placental IL-12 level was higher in the pregnant women with acute TOX compared with controls (P = 0.001). SP ratio of MEL was higher in the pregnant women with acute TOX compared with controls (P = 0.001). Conclusion: MEL and anti-inflammatory IL-10 are reduced in the pregnant women with acute TOX, while IL-12 is increased. SP ratio of MEL but not of IL-10 or IL-12 is elevated in the pregnant women with acute TOX reflecting the risk of PD.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"87-91"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cf/c0/JMAU-11-87.PMC10337677.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epidemiological, Clinical, and Histopathological Features of Salivary Gland Tumors among 150 Sudanese Patients: 10 Years' Experience.","authors":"Somaya T S Hamid,&nbsp;Mohammed A M Abdalla,&nbsp;Azza A Zulfu,&nbsp;Azza Ramadan,&nbsp;Musaab Ahmed,&nbsp;Mohamed H Ahmed","doi":"10.4103/jmau.jmau_113_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_113_20","url":null,"abstract":"<p><strong>Background and objectives: </strong>Salivary gland tumors (SGTs) are serious challenges to pathologists. Herein, we aimed to assess epidemiological and histopathological characteristics of SGTs among Sudanese patients.</p><p><strong>Materials and methods: </strong>This retrospective descriptive study was undertaken at The pathology department in Khartoum State between 2008 and 2018. Patient records, histopathological reports, and slides were retrieved; and re-examined by two histopathologists. Diagnoses were reclassified according to the 2017 WHO classification of SGTs.</p><p><strong>Results: </strong>Overall, 150 cases of Sudanese patients with SGT were included (90 [60%] males and 60 [40%] females). Among these, 105 were benign (70%) and 45 were malignant (30%). The parotid glands were the most common site for both benign and malignant tumors (77/150; 51%: 59 benign (76.6%) and 18 malignant [23.4%]). The next common site was the submandibular gland (54 [36%]: 38 benign [70.3%] and 16 malignant [29.7%]), followed by minor salivary glands (19 [12.7%]: 8 benign and 11 malignant [57.9%]). Benign gland entities included pleomorphic adenoma (88/105; 83.7%), oncocytoma (5/105; 4.8%), myoepithelioma (4/105; 3.8%), Whartin tumors (3/105; 2.9%), basal cell adenoma (3/105; 2.9%), and sialolipoma (2/105; 1.9%). Malignant gland entities included adenoid cystic carcinoma (12; 26.7%), mucoepidermoid carcinoma (10; 22,2%), acinic cell carcinoma (6; 13.3%), poorly differentiated carcinoma (4; 8.9%), adenocarcinoma NOS (not otherwise specified) (4; 8.9%), basal cell adenocarcinoma (3; 6.7%), carcinoma ex pleomorphic adenoma (3; 6.7%), polymorphous adenocarcinoma (2; 4.4%), salivary duct carcinoma (1; 2.2%), and epithelial-myoepithelial carcinoma (2.2%).</p><p><strong>Conclusions: </strong>SGTs shared several epidemiological and histopathological features, exhibiting high incidence in the parotid and submandibular glands, lower prevalence in minor glands, and greater male predominance.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"92-96"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/3d/JMAU-11-92.PMC10337678.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9825869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Correlation of Serum Histamine and Mast Cell Count with the Established Prognosticators in Oral Cancer.","authors":"Nabeel Rashid Hasan,&nbsp;Priyadarshini Dehuri,&nbsp;Amitabh Jena,&nbsp;Srikanta Kanungo","doi":"10.4103/jmau.jmau_138_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_138_20","url":null,"abstract":"<p><strong>Context: </strong>We undertook this study in view of the rising interest in the interactions between tumor cells and mast cells in the tumor microenvironment and the mounting evidence of the role of mast cells in the prognosis of various cancers.</p><p><strong>Aims: </strong>The aim of the study is to determine the prognostic utility of mast cells and their mediators in oral squamous cell carcinoma (OSCC).</p><p><strong>Settings and design: </strong>This is a cross-sectional analytical study. A total of 41 cases were studied for determining the correlation of mast cell counts with histopathological prognostic variables and 29 cases for determining the serum histamine levels and to further correlate the values with the histopathologic determinants.</p><p><strong>Subjects and methods: </strong>Mast cell assessment was done using Toluidine blue stain on tissue sections while histopathological prognostic factors were evaluated with routine stains. The serum histamine levels were obtained by sandwich enzyme linked immunosorbent assay (ELISA).</p><p><strong>Statistical analysis: </strong>Mann-Whitney <i>U</i>-test was used to test the difference between parameters and a Spearman correlation coefficient was used. <i>P</i> < 0.05 was considered significant for statistical analysis.</p><p><strong>Results: </strong>We found statistically significant correlations between increased median mast cell counts and higher grade of tumor, presence of lymphovascular invasion, greater depth of invasion, and presence of regional lymph node involvement. The serum histamine values correlated only with the depth of tumor invasion.</p><p><strong>Conclusions: </strong>Hence, as per our results, we suggest the use of mast cell counts in OSCC cases as a prognostic indicator in conjunction with other clinicopathological factors. At the same time, we acknowledge the fact that extensive studies are required to establish the role of mast cell mediators in the prognostic curve of oral cancer patients.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"97-102"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d9/02/JMAU-11-97.PMC10337676.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rare Presentation of Multifocal Anaplastic Oligodendroglioma.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_126_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_126_20","url":null,"abstract":"<p><p>Multifocal tumors are usually reported within the same cerebral hemisphere due to widespread dissemination along the white matter tracts. This case report describes the magnetic resonance imaging appearances of multifocal anaplastic oligodendroglioma in a 28-year-old adult male that showed three discrete heterogeneously enhancing cortical-based lesions in the left frontoparietal lobes. Left frontal craniotomy was performed and biopsy of the lesion was obtained, histopathology of which showed features of anaplastic oligodendroglioma.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"115-117"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e2/60/JMAU-11-115.PMC10337670.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.","authors":"Heba A Mahmoud,&nbsp;Hemat E El Horany,&nbsp;Marwa Aboalsoud,&nbsp;Rania Nagi Abd-Ellatif,&nbsp;Amal Ahmed El Sheikh,&nbsp;Alshimaa Aboalsoud","doi":"10.4103/jmau.jmau_78_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_78_22","url":null,"abstract":"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold Nanoparticles Down-Regulate Alpha Fetoprotein Expression Induced by Meloxicam Hepatotoxicity in Adult Male Albino Rats: Histological and Immunohistochemical Study.","authors":"Hekmat Osman Abdel Aziz, Mai Nashat, Aziz Awaad, Sherine Ahmed Mohammed","doi":"10.4103/jmau.jmau_109_22","DOIUrl":"10.4103/jmau.jmau_109_22","url":null,"abstract":"<p><strong>Background: </strong>Meloxicam is a non-steroidal anti-inflammatory drug most commonly used for the treatment of arthritis. Meloxicam decreases prostaglandin E2 resulting in an increase in free radical concentration within the cell. Alpha-fetoprotein (AFP) is a protein produced normally by the fetal liver in hepatoblasts. In inflammatory conditions, the adult liver synthesizes AFP by regenerating cells. Gold nanoparticles (AuNPs) in the medical field, represent one of the most commonly studied metal nanoparticles which have antioxidant properties.</p><p><strong>Objective: </strong>This study was conducted to evaluate the possible therapeutic effects of AuNPs on Meloxicam induced degenerative changes in rat liver.</p><p><strong>Materials and methods: </strong>fifty adult male albino rats were divided into 8 groups: The first group (control); the AuNPs group was treated with AuNPs daily for 2 weeks. The MEL 2w& MEL 2m groups were treated with meloxicam daily for 2 weeks and 2 months respectively. The MEL2w+AuNPs & MEL2m+AuNPs groups received AuNPs for 2 weeks after meloxicam injection daily for 2 weeks and 2 months respectively. The MEL2w+SAL & MEL2m+SAL groups were given meloxicam for 2 weeks and 2 months respectively followed by saline injection for 2 weeks. Histological changes, AuNPs localization in the liver by silver nitrate stain, and AFP immunoexpression were studied.</p><p><strong>Results: </strong>Time dependent Degenerative changes and increased AFP expression were observed in the liver after meloxicam injection. However, AuNPs ameliorated these changes and decreased AFP expression. AuNPs were detected in Kupffer cells.</p><p><strong>Conclusion: </strong>AuNPs could ameliorate meloxicam-induced toxicity in the liver and decrease AFP expression because AuNPs act as free radical scavengers which accumulate in Kupffer cells.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"114 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Large Retroperitoneal Tumor Operated for Suspected Renal Cell Carcinoma Turned Out to be Primary Renal Synovial Sarcoma: A Rare Entity.","authors":"Pranita Mohanty, Surabhi Singh, Pallak Batalia","doi":"10.4103/jmau.jmau_72_22","DOIUrl":"10.4103/jmau.jmau_72_22","url":null,"abstract":"<p><p>Approximately 5%-10% of soft-tissue tumor comprised synovial sarcoma (SS) and are commonly found in close proximity of large joints affecting young adults. However, SS of the kidney is extremely unusual (1%) and has a poor prognosis. There are three subtypes of primary renal SS (PRSS). The monophasic variant is the most common and shares the same clinical, molecular, and ultrastructural features with other subtypes. Histomorphology with immunohistochemistry (IHC) differentiates it from other sarcomas of kidney and sarcomatoid renal cell carcinoma. Genetic analysis reveals specific SYT-SSX2 (X, 18) translocation. Herein, a case of PRSS (monophasic variant) is presented in a 31-year-old female with right-sided loin pain and huge mass of size (11.9 cm × 9.3 cm × 10 cm) without associated hematuria. It was clinically thought to be renal cell carcinoma. Radical nephrectomy was performed, followed by histopathology and IHC which suggested PRSS. Then, combined chemotherapy of doxorubicin with cyclophosphamide was rendered, and on follow-up, she is doing well with an uneventful course.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"109-111"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12236405/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Buccal Exfoliated Cells in Individuals with Diabetes Mellitus and Healthy Controls: A Cytomorphometric Analysis.","authors":"H Nanda Kumar, Bose Divya, Annasamy Ramesh Kumar, Madhu Narayan, V Vasanthi, Ramya Ramadoss, Muthulakshmi Chandrasekar","doi":"10.4103/jmau.jmau_82_22","DOIUrl":"10.4103/jmau.jmau_82_22","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus is the third most frequent cause of mortality and morbidity worldwide. Patients with diabetes exhibit a variety of oral symptoms, and hence the early detection of this condition can be addressed by a dentist.</p><p><strong>Aim: </strong>The current study aimed to study the cytomorphometric alterations of buccal exfoliated cells in individuals with type II diabetes mellitus.</p><p><strong>Methodology: </strong>The study included thirty diabetics and thirty healthy controls. The smears were obtained from the buccal mucosa and stained with Papanicolaou stain and hematoxylin and eosin stain. The presence of inflammatory cells, microbial carriage, nuclear enlargement, and perinuclear halo and binucleation were examined on the slides. Cellular and nuclear parameters were quantitatively measured using Image J software. Statistical analysis was done using SPSS software, and the Student's <i>t</i>-test was employed.</p><p><strong>Results: </strong>No inflammatory cells or microbes were observed in Group I individuals; however, the perinuclear halo was observed in 16.6% and binucleated cells in 3.3% of the controls. Inflammatory cells, consisting mainly of neutrophils and lymphocytes were seen in 40%, microbial carriage in 26.6%, perinuclear halo in 73.3%, and binucleated cells in 36.6% of the diabetic patients. The mean nuclear diameter, area, and nuclear-cytoplasmic ratio were significantly high in diabetic patients when compared to healthy controls.</p><p><strong>Conclusion: </strong>Oral exfoliated mucosal cells of patients with diabetes mellitus exhibit distinct cytomorphometric alterations such as increased nuclear diameter, nuclear area, and nuclear-cytoplasmic ratio.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"185-189"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48638667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Utility of Gram Stain for Oral Smears - A Review.","authors":"Tharani Vijayakumar, Bose Divya, V Vasanthi, Madhu Narayan, Annasamy Ramesh Kumar, Rajkumar Krishnan","doi":"10.4103/jmau.jmau_108_22","DOIUrl":"10.4103/jmau.jmau_108_22","url":null,"abstract":"<p><p>For rapid and successful treatment of infectious diseases, detection of the presence of microorganisms is essential. Traditional culture-based approaches are limiting and time consuming for microbial identification. The most popular staining technique for identifying Gram-positive and Gram-negative microorganisms in various tissues is called Gram staining. This method is utilized in both clinical practice and research. Gram staining of the oral smears is the preliminary step in the identification of any pathological shift in normal oral microbiota. This review discusses the principle of gram stain emphasizing its significance in diagnostic utility for oral smears.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"130-134"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48370578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Ultrastructural Morphological Characterization of Platelet-Rich Fibrin among Diabetics.","authors":"Vinaya Rudresh, Suchetha Aghanashini, S Srirangarajan, P Srikumar, Ravi J Rao, B Upasana","doi":"10.4103/jmau.jmau_103_22","DOIUrl":"10.4103/jmau.jmau_103_22","url":null,"abstract":"<p><strong>Background: </strong>The basic biology of the Platelet Rich Fibrin lies in the fact that they have platelets with enclosed granules in them and fibrin with the ability to release growth factors.</p><p><strong>Aims and objective: </strong>The platelet rich fibrin electron microscopic structure has been characterized in the present cross-sectional novel study. This unique biologic material being vastly used since four decades is not microscopically analyzed amongst type II diabetes mellitus subjects, using light microscopy and transmission electron microscopy. The present novel work employs the transmission electron microscope use to reveal the characteristics of cells in order to correlate the growth factor release.</p><p><strong>Materials and methods: </strong>Venous blood samples drawn were subjected to analysis of HbA1c, CBC, platelet indices, and PRF membrane preparation. Platelet rich fibrin membranes were prepared from healthy, well-controlled, and poorly-controlled diabetic individuals following the protocols for Advanced-Platelet Rich Fibrin+ (1,300 rpm, 8 min) and subjected to assessment of morphological analysis using a light microscope, transmission electron microscopy and growth factor release.</p><p><strong>Results: </strong>A denser network of fibrin with highest growth factor release was seen in the present study.</p><p><strong>Conclusion: </strong>Hence, with the highest growth factors release and a denser network of fibrin, this novel study finds promising biomaterial in diabetics.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}