Journal of Microscopy and Ultrastructure最新文献

{"title":"A Rare Presentation of Multifocal Anaplastic Oligodendroglioma.","authors":"Ravikanth Reddy","doi":"10.4103/jmau.jmau_126_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_126_20","url":null,"abstract":"<p><p>Multifocal tumors are usually reported within the same cerebral hemisphere due to widespread dissemination along the white matter tracts. This case report describes the magnetic resonance imaging appearances of multifocal anaplastic oligodendroglioma in a 28-year-old adult male that showed three discrete heterogeneously enhancing cortical-based lesions in the left frontoparietal lobes. Left frontal craniotomy was performed and biopsy of the lesion was obtained, histopathology of which showed features of anaplastic oligodendroglioma.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"115-117"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e2/60/JMAU-11-115.PMC10337670.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9820461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.","authors":"Heba A Mahmoud,&nbsp;Hemat E El Horany,&nbsp;Marwa Aboalsoud,&nbsp;Rania Nagi Abd-Ellatif,&nbsp;Amal Ahmed El Sheikh,&nbsp;Alshimaa Aboalsoud","doi":"10.4103/jmau.jmau_78_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_78_22","url":null,"abstract":"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"107-114"},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9823280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold Nanoparticles Down-Regulate Alpha Fetoprotein Expression Induced by Meloxicam Hepatotoxicity in Adult Male Albino Rats: Histological and Immunohistochemical Study.","authors":"Hekmat Osman Abdel Aziz, Mai Nashat, Aziz Awaad, Sherine Ahmed Mohammed","doi":"10.4103/jmau.jmau_109_22","DOIUrl":"10.4103/jmau.jmau_109_22","url":null,"abstract":"<p><strong>Background: </strong>Meloxicam is a non-steroidal anti-inflammatory drug most commonly used for the treatment of arthritis. Meloxicam decreases prostaglandin E2 resulting in an increase in free radical concentration within the cell. Alpha-fetoprotein (AFP) is a protein produced normally by the fetal liver in hepatoblasts. In inflammatory conditions, the adult liver synthesizes AFP by regenerating cells. Gold nanoparticles (AuNPs) in the medical field, represent one of the most commonly studied metal nanoparticles which have antioxidant properties.</p><p><strong>Objective: </strong>This study was conducted to evaluate the possible therapeutic effects of AuNPs on Meloxicam induced degenerative changes in rat liver.</p><p><strong>Materials and methods: </strong>fifty adult male albino rats were divided into 8 groups: The first group (control); the AuNPs group was treated with AuNPs daily for 2 weeks. The MEL 2w& MEL 2m groups were treated with meloxicam daily for 2 weeks and 2 months respectively. The MEL2w+AuNPs & MEL2m+AuNPs groups received AuNPs for 2 weeks after meloxicam injection daily for 2 weeks and 2 months respectively. The MEL2w+SAL & MEL2m+SAL groups were given meloxicam for 2 weeks and 2 months respectively followed by saline injection for 2 weeks. Histological changes, AuNPs localization in the liver by silver nitrate stain, and AFP immunoexpression were studied.</p><p><strong>Results: </strong>Time dependent Degenerative changes and increased AFP expression were observed in the liver after meloxicam injection. However, AuNPs ameliorated these changes and decreased AFP expression. AuNPs were detected in Kupffer cells.</p><p><strong>Conclusion: </strong>AuNPs could ameliorate meloxicam-induced toxicity in the liver and decrease AFP expression because AuNPs act as free radical scavengers which accumulate in Kupffer cells.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"114 1","pages":"8-15"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Buccal Exfoliated Cells in Individuals with Diabetes Mellitus and Healthy Controls: A Cytomorphometric Analysis.","authors":"H Nanda Kumar, Bose Divya, Annasamy Ramesh Kumar, Madhu Narayan, V Vasanthi, Ramya Ramadoss, Muthulakshmi Chandrasekar","doi":"10.4103/jmau.jmau_82_22","DOIUrl":"10.4103/jmau.jmau_82_22","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus is the third most frequent cause of mortality and morbidity worldwide. Patients with diabetes exhibit a variety of oral symptoms, and hence the early detection of this condition can be addressed by a dentist.</p><p><strong>Aim: </strong>The current study aimed to study the cytomorphometric alterations of buccal exfoliated cells in individuals with type II diabetes mellitus.</p><p><strong>Methodology: </strong>The study included thirty diabetics and thirty healthy controls. The smears were obtained from the buccal mucosa and stained with Papanicolaou stain and hematoxylin and eosin stain. The presence of inflammatory cells, microbial carriage, nuclear enlargement, and perinuclear halo and binucleation were examined on the slides. Cellular and nuclear parameters were quantitatively measured using Image J software. Statistical analysis was done using SPSS software, and the Student's <i>t</i>-test was employed.</p><p><strong>Results: </strong>No inflammatory cells or microbes were observed in Group I individuals; however, the perinuclear halo was observed in 16.6% and binucleated cells in 3.3% of the controls. Inflammatory cells, consisting mainly of neutrophils and lymphocytes were seen in 40%, microbial carriage in 26.6%, perinuclear halo in 73.3%, and binucleated cells in 36.6% of the diabetic patients. The mean nuclear diameter, area, and nuclear-cytoplasmic ratio were significantly high in diabetic patients when compared to healthy controls.</p><p><strong>Conclusion: </strong>Oral exfoliated mucosal cells of patients with diabetes mellitus exhibit distinct cytomorphometric alterations such as increased nuclear diameter, nuclear area, and nuclear-cytoplasmic ratio.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"185-189"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48638667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Utility of Gram Stain for Oral Smears - A Review.","authors":"Tharani Vijayakumar, Bose Divya, V Vasanthi, Madhu Narayan, Annasamy Ramesh Kumar, Rajkumar Krishnan","doi":"10.4103/jmau.jmau_108_22","DOIUrl":"10.4103/jmau.jmau_108_22","url":null,"abstract":"<p><p>For rapid and successful treatment of infectious diseases, detection of the presence of microorganisms is essential. Traditional culture-based approaches are limiting and time consuming for microbial identification. The most popular staining technique for identifying Gram-positive and Gram-negative microorganisms in various tissues is called Gram staining. This method is utilized in both clinical practice and research. Gram staining of the oral smears is the preliminary step in the identification of any pathological shift in normal oral microbiota. This review discusses the principle of gram stain emphasizing its significance in diagnostic utility for oral smears.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"130-134"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679832/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48370578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Ultrastructural Morphological Characterization of Platelet-Rich Fibrin among Diabetics.","authors":"Vinaya Rudresh, Suchetha Aghanashini, S Srirangarajan, P Srikumar, Ravi J Rao, B Upasana","doi":"10.4103/jmau.jmau_103_22","DOIUrl":"10.4103/jmau.jmau_103_22","url":null,"abstract":"<p><strong>Background: </strong>The basic biology of the Platelet Rich Fibrin lies in the fact that they have platelets with enclosed granules in them and fibrin with the ability to release growth factors.</p><p><strong>Aims and objective: </strong>The platelet rich fibrin electron microscopic structure has been characterized in the present cross-sectional novel study. This unique biologic material being vastly used since four decades is not microscopically analyzed amongst type II diabetes mellitus subjects, using light microscopy and transmission electron microscopy. The present novel work employs the transmission electron microscope use to reveal the characteristics of cells in order to correlate the growth factor release.</p><p><strong>Materials and methods: </strong>Venous blood samples drawn were subjected to analysis of HbA1c, CBC, platelet indices, and PRF membrane preparation. Platelet rich fibrin membranes were prepared from healthy, well-controlled, and poorly-controlled diabetic individuals following the protocols for Advanced-Platelet Rich Fibrin+ (1,300 rpm, 8 min) and subjected to assessment of morphological analysis using a light microscope, transmission electron microscopy and growth factor release.</p><p><strong>Results: </strong>A denser network of fibrin with highest growth factor release was seen in the present study.</p><p><strong>Conclusion: </strong>Hence, with the highest growth factors release and a denser network of fibrin, this novel study finds promising biomaterial in diabetics.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2023-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063922/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Possible Ameliorating Role of Fisetin on Hepatic Changes Induced by Fluoxetine in Adult Male Albino Rats: Histological, Immunohistochemical, and Biochemical Study.","authors":"Dina Fouad El Shaer, Hend Ibrahim Abd El Halim","doi":"10.4103/jmau.jmau_84_22","DOIUrl":"10.4103/jmau.jmau_84_22","url":null,"abstract":"<p><strong>Background: </strong>Fluoxetine (FLX) is one of the selective serotonin reuptake inhibitors, it is widely used to treat neuropsychiatric disorders including depression, but high doses can cause several adverse effects. Fisetin (FIS), a bioactive flavonoid presents in vegetables and fruits, has antioxidant, anti-inflammatory, and anticancer effects.</p><p><strong>Aim: </strong>To evaluate the possible ameliorating effect of FIS on the hepatic alterations induced by FLX in adult male albino rats.</p><p><strong>Materials and methods: </strong>Our study was done, for 3-weeks, on 48 rats that were divided into four groups: Group I (control), Group II received FIS orally (100 mg/kg/day), Group III received FLX orally (10 mg/kg/day), and Group IV concomitantly received FLX and FIS at the same dose and manner of groups II and III. Blood and liver samples were obtained and prepared for histological, immunohistochemical, and biochemical studies.</p><p><strong>Results: </strong>FLX group revealed disturbed liver architecture, hepatocytes with vacuolated cytoplasm, inflammatory cellular infiltration, blood extravasation, and congestion of blood vessels in addition to, a significant increase in the area percentage of caspase-3, inducible nitric oxide synthase and the number of glial fibrillary acidic protein-expressing cells as well as a significant decrease in the area percentage of periodic acid-Schiff stain. Moreover, FLX significantly increased aspartate-aminotransferase and alanine-aminotransferase levels in the serum. In addition, FLX increased malondialdehyde level and decreased superoxide dismutase, glutathione (GSH) peroxidase, and reduced GSH levels in liver tissue. The concomitant administration of FIS ameliorated these alterations.</p><p><strong>Conclusions: </strong>Administration of FIS ameliorated the histological, immunohistochemical, and biochemical alterations induced by FLX in the liver of adult male albino rats.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"161-171"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10679833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42117089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in Glial Fibrillary Acidic Protein-Immunoreactive Astrocytes in the Prefrontal Cortex of the Male Rat following Chronic Khat Use.","authors":"Paul Bundi Karau, Paul Odula, Moses Obimbo, Amadi Ogonda Ihunwo, Pilani Nkomozepi, Muriira Karau","doi":"10.4103/jmau.jmau_26_22","DOIUrl":"10.4103/jmau.jmau_26_22","url":null,"abstract":"<p><strong>Background: </strong>Long-term khat consumption is associated with significant neurocognitive changes, which have been elucidated in behavioral studies. With current research showing the centrality of astrocytes and other glial cells in neuronal signaling, there is possibility that these cells are also affected by chronic khat use. There is little literature on the structural changes in the prefrontal cortex neuronal and astrocytic cytoarchitecture and morphometry in chronic khat users.</p><p><strong>Objective: </strong>The objective of this study was to describe the changes in astrocyte morphometry and structure in rats after long-term use of khat (miraa).</p><p><strong>Materials and methods: </strong>Adult male Wistar rats, aged 2-3 months, weighing 200-300 g were randomized into four groups of 10 each (control, Group 1, Group 2, and Group 3) to correspond with those used as controls and those that received 500 mg/kg, 1000 mg/kg, and 2000 mg/kg body weight khat extracts, respectively. Fresh khat leaves were purchased from Maua market in Meru, and crude extract was prepared using lyophilization. The control rats were fed on normal diet, while the experimental groups were fed on normal diet and khat extracts using oral gavage for 6 weeks. The animals were sacrificed and their brains were removed. We performed immunohistochemical visualization of astrocytes using glial fibrillary acidic protein. Photomicrographs of the stained sections were transferred to ImageJ Fiji software to study the astrocyte density and astrocytic processes. We used Kruskal-Wallis test to correlate the four animal groups in terms of astrocyte densities.</p><p><strong>Results: </strong>We observed an increase in the average number of astrocytes with increasing doses of khat compared to controls, with those in Group 3 (2000 mg/kg) having an exuberant reactive astrocytosis. Further, escalating khat doses resulted in increased glial fibrillary acidic protein immunoreactivity in the nuclei and astrocytic processes, gliotic changes, and increased complexity of astrocytic processes.</p><p><strong>Conclusion: </strong>Chronic khat use, especially at high doses, results in reactive astrocytosis and astrogliosis, which may be part of the mechanisms involved in the cognitive changes associated with its use.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"75-80"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245132/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Adrenal Progenitor Cells in the Structural Response of Adrenal Gland to Various forms of Acute Stress and Subsequent Recovery in Adult Male Albino Rats.","authors":"Doaa Ramadan Sadek, Seham Kamel Abunasef, Sahar Khalil","doi":"10.4103/jmau.jmau_106_22","DOIUrl":"10.4103/jmau.jmau_106_22","url":null,"abstract":"<p><strong>Background: </strong>Stress is a response to stressogenic stimuli that interferes with an organism's homeostasis. The adrenal gland is crucial in the body's reaction to stress.</p><p><strong>Objective: </strong>This study compared the effects of immobility and cold as acute stressors and the subsequent recovery on the histological changes of the adrenal gland and the suspected role of the adrenal progenitor cells.</p><p><strong>Materials and methods: </strong>Thirty-five adult male albino rats were divided equally into five groups. Group I: Control group, Group II: Rats subjected to the acute cold stress procedure, Group III: Rats subjected to the acute immobilization stress procedure, Group IV: The combined stress group, and Group V: Similar to the combined stress group and recovered for 6 days then sacrificed 1 day later. Serum cortisol level was determined, and the adrenal glands were processed for histological and immunohistochemical studies.</p><p><strong>Results: </strong>Serum cortisol concentration was higher in the acute-stress groups and decreased in the recovery group. The adrenal cortex had enlarged, vacuolated cells with pyknotic nuclei, sinusoidal dilatation, and congestion. Chromaffin cells were crowded, enlarged, and vacuolated. There was strong immunohistochemical reactivity for heat shock protein-70 and caspase-3. In addition, the combined group showed a significant increase in the optical density of chromogranin-A in the medullary cells as well as CD44+ve cells. These findings were decreased in the recovery group.</p><p><strong>Conclusions: </strong>The combined stress has more deleterious adrenal cortical changes than immobilization and cold stress alone. The progenitor and chromaffin cells apparently had an important regenerative role in recovery from both types of stress.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"16-24"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12063925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Diagnostic Challenge of Sialolithiasis.","authors":"Sahar M N Bukhary","doi":"10.4103/jmau.jmau_92_22","DOIUrl":"10.4103/jmau.jmau_92_22","url":null,"abstract":"<p><p>The major salivary glands (parotid, submandibular, and sublingual) are most frequently obstructed by calculi within the salivary gland, or more uncommonly, by ranulas. Despite the well-defined clinical and radiographic diagnostic features, sialolithiasis may sometimes be confused with sialadenitis and ranulas, especially when encountered in general dental practice. We, therefore, present a case that illustrates this diagnostic dilemma to highlight the salient features of all three conditions. A 28-year-old female presented with a history of a submandibular swelling for 8 months. On intraoral examination, a bluish sublingual swelling was identified at the left side of the lingual frenum, causing a slight elevation of the tongue. The preliminary diagnosis was of a ranula; however, the clinical history suggested sialolithiasis. A hard structure was palpated in the submandibular gland, and a mandibular occlusal film revealed a large ductal sialolith. Sialolithotomy was performed under local anesthesia, and a single 7.2 mm stone was retrieved. The postoperative follow-up period was uneventful, with good healing and restored normal salivary flow. Despite the fairly clear clinical and radiographic diagnostic criteria suggestive of sialolithiasis, the bluish-tinged swelling of the floor of the mouth prompted the examining dentist to provisionally diagnose a ranula. Sialolithiasis is a common obstructive condition of the salivary gland encountered in the dental setting. Despite the clinical and radiographic features usually guiding the correct diagnosis, it can be a challenging diagnosis for less experienced dentists, who must always carefully consider the history, clinical, and radiographic findings.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"221-224"},"PeriodicalIF":0.0,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}