Journal of Microscopy and Ultrastructure最新文献

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Tissue Microarray for Gynecological Pathology Studies: A Mini-Review. 组织微阵列用于妇科病理研究:综述。
Journal of Microscopy and Ultrastructure Pub Date : 2026-01-09 eCollection Date: 2026-01-01 DOI: 10.4103/jmau.jmau_141_24
Mohammad Arafa, Abd AlRahman Foda, Amany Salama, Ola Shalaby, Muna Al-Jabri, Fatma Al Hinai, Afrah Al-Rashdi, Samya Al-Husaini, Suaad Al-Badi
{"title":"Tissue Microarray for Gynecological Pathology Studies: A Mini-Review.","authors":"Mohammad Arafa, Abd AlRahman Foda, Amany Salama, Ola Shalaby, Muna Al-Jabri, Fatma Al Hinai, Afrah Al-Rashdi, Samya Al-Husaini, Suaad Al-Badi","doi":"10.4103/jmau.jmau_141_24","DOIUrl":"https://doi.org/10.4103/jmau.jmau_141_24","url":null,"abstract":"<p><p>The tissue microarray (TMA) was created to enhance the efficiency of molecular profiling in cancer research through the rapid execution of large-scale studies. This technology facilitates the simultaneous examination of proteins or genes on a single glass slide, that contains an array of hundreds of paraffin-embedded tissue cores. TMAs can be assessed using immunohistochemistry and <i>in situ</i> hybridization techniques. They allow the analysis of both normal and cancerous tissues, making them particularly valuable for studies aimed at validating cancer biomarkers. This article will focus on the application of TMA within the field of gynecological pathology. Suggestions for careful considerations to avoid the typical challenges associated with this technology are also provided.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"14 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2026-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effects of Ellagic Acid on Hemoglobin Ingestion, Transport, and Digestion in the Digestive Vacuole of Plasmodium falciparum. 鞣花酸对恶性疟原虫消化液泡中血红蛋白摄取、运输和消化的影响。
Journal of Microscopy and Ultrastructure Pub Date : 2024-11-28 eCollection Date: 2025-10-01 DOI: 10.4103/jmau.jmau_96_24
Anjana Chamilka Thuduhena, Nurhidanatasha Abu-Bakar, Siti Nasuha Hamzah
{"title":"Effects of Ellagic Acid on Hemoglobin Ingestion, Transport, and Digestion in the Digestive Vacuole of <i>Plasmodium falciparum</i>.","authors":"Anjana Chamilka Thuduhena, Nurhidanatasha Abu-Bakar, Siti Nasuha Hamzah","doi":"10.4103/jmau.jmau_96_24","DOIUrl":"10.4103/jmau.jmau_96_24","url":null,"abstract":"<p><strong>Context: </strong>Malaria, caused by <i>Plasmodium</i> parasites, has been a persistent threat to human health for centuries, causing significant illnesses and fatalities. The rise of drug-resistant strains has diminished the efficacy of conventional antimalarial medications, necessitating the exploration of alternative treatments. Ellagic acid has emerged as a promising candidate, demonstrating effectiveness in laboratory experiments by impeding beta-hematin (hemozoin) formation in mature <i>Plasmodium falciparum</i> stages. This research endeavors to elucidate the ellagic acid's mechanism of action against malaria parasites by examining its impact on hemoglobin ingestion, transport, and digestion in both chloroquine-sensitive (3D7) and chloroquine-resistant (W2) <i>P. falciparum</i> strains <i>in vitro</i>.</p><p><strong>Aim: </strong>This study aimed to elucidate the mechanism of ellagic acid antimalarial action against 3D7 and W2 strains of <i>P. falciparum in vitro</i> by using a transmission electron microscope.</p><p><strong>Materials and methods: </strong>3D7 and W2 strains of <i>P. falciparum</i> were cultured in flasks with a complete culture medium and washed red blood cells (type O<sup>+</sup>) at a hematocrit of 2%. Synchronized ring-stage parasite-infected erythrocytes, approximately 2 h post-synchronization, were utilized for the antimalarial activity assay. Based on the half-maximal inhibitory concentration (IC<sub>50</sub>) value of ellagic acid, three different sub-lethal concentrations of the compound against the 3D7 and W2 strains. Samples were prepared for real-time microscopic analysis. Samples were immediately fixed in 2.5% glutaraldehyde in 0.2 M phosphate buffer (pH 7.4) for 2 h. Then, the samples were washed, postfixed with 2% osmium tetroxide in 0.1 M phosphate buffer for 1 h, washed with distilled water, dehydrated, infiltrated, and embedded. The sample blocks were cut by using an ultramicrotome. These thin sections were stained with 1% lead citrate and 1% uranyl acetate.</p><p><strong>Statistical analysis used: </strong>The IC<sub>50</sub> of ellagic acid against the 3D7 and W2 parasites was analyzed using computerized GraphPad Prism 8.0 software.</p><p><strong>Results: </strong>Findings show significant inhibition by ellagic acid (<i>P</i> < 0.001) against 3D7 and W2 parasites. The IC<sub>50</sub> values were 1.0 ± 0.7 and 1.3 ± 0.1 nM, respectively, surpassing the artemisinin's effectiveness (IC<sub>50-3D7 parasite</sub> = 2.2 ± 0.2 nM; IC<sub>50-W2 parasite</sub> = 4.3 ± 0.3 nM). Transmission electron microscopy elucidated the ellagic acid's impact on the early trophozoite stage of 3D7 and W2 parasites, highlighting the disruption of hemoglobin metabolism within the parasites.</p><p><strong>Conclusion: </strong>These findings underscore the ellagic acid's potential as a promising antimalarial agent, targeting hemoglobin processes in both 3D7 and W2 <i>P. falciparum</i> strains.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"13 4","pages":"210-226"},"PeriodicalIF":0.0,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12710731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145781112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum: Stereological and Histological Assessment of the Umbilical Cord in New-Born Rat. 勘误:新生大鼠脐带的立体学和组织学评估
Journal of Microscopy and Ultrastructure Pub Date : 2024-09-17 eCollection Date: 2024-07-01 DOI: 10.4103/JMAU.JMAU_98_24
{"title":"Erratum: Stereological and Histological Assessment of the Umbilical Cord in New-Born Rat.","authors":"","doi":"10.4103/JMAU.JMAU_98_24","DOIUrl":"10.4103/JMAU.JMAU_98_24","url":null,"abstract":"<p><p>[This corrects the article on p. 108 in vol. 9, PMID: 34729350.].</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"12 3","pages":"169"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the Impact of Surface Modification with Air Abrasion (sandblasting) and Ultraviolet Radiation on the Retention Strength of Polyetherether Ketone (PEEK) Coping in Relation to the Underlying Dentin Abutments: An in vitro Study. 空气磨蚀(喷砂)和紫外线表面改性对聚醚酮(PEEK)顶部与牙本质基牙的保持强度影响的体外研究
Journal of Microscopy and Ultrastructure Pub Date : 2024-09-02 eCollection Date: 2026-01-01 DOI: 10.4103/jmau.jmau_80_24
Akash Mithran, Vandana Rajput Upwanshi, Ismail Mohammed Alqahtani, Sultan Jaber M Alqahtani, Prajna P Nayak, Abdulhakim Ali Alsharif, J Suresh Babu, C Swarnalatha, Abhishek Singh Nayyar
{"title":"Evaluation of the Impact of Surface Modification with Air Abrasion (sandblasting) and Ultraviolet Radiation on the Retention Strength of Polyetherether Ketone (PEEK) Coping in Relation to the Underlying Dentin Abutments: An <i>in vitro</i> Study.","authors":"Akash Mithran, Vandana Rajput Upwanshi, Ismail Mohammed Alqahtani, Sultan Jaber M Alqahtani, Prajna P Nayak, Abdulhakim Ali Alsharif, J Suresh Babu, C Swarnalatha, Abhishek Singh Nayyar","doi":"10.4103/jmau.jmau_80_24","DOIUrl":"https://doi.org/10.4103/jmau.jmau_80_24","url":null,"abstract":"<p><strong>Objective: </strong>The present study aims to evaluate the effect of surface modifications of polyetherether ketone (PEEK) with air abrasion (sandblasting) and ultraviolet radiation (UV radiation) on the retention strength of PEEK copings in relation to the underlying dentin abutments.</p><p><strong>Settings and design: </strong>The present study is an <i>in vitro</i> study design.</p><p><strong>Materials and methods: </strong>For the present <i>in vitro</i> study, 36 non-carious, intact maxillary premolars were collected, and the specimens were divided into three groups: including Group A, wherein surface modification was accomplished with air abrasion (sandblasting); Group B, wherein surface modification was accomplished using 172-308 nm UV radiation; and Group C, wherein surface modification was accomplished using air abrasion (sandblasting), followed by treatment with UV radiation. The PEEK copings, bonded to the underlying dentin abutments, were subjected to a pull-off test using the Hounsfield universal testing machine, while the maximum load (force) value, at which debonding of the copings from specimens was observed, was recorded and subjected to statistical analysis. Due to the non-normal distribution of the variables, subsequent multiple comparisons between different groups were performed using Kruskal-Wallis <i>H</i>-test. Also, <i>post hoc</i> analysis was performed using the Mann-Whitney <i>U</i>-test with Bonferroni correction applied to conduct pairwise comparisons among independent groups.</p><p><strong>Results: </strong>Kruskal-Wallis <i>H</i>-test used revealed a significant difference among all the test groups (<i>P</i> < 0.001), while variations in the median retention strength values obtained were found to be statistically significant when compared between Group A and Group C specimens (<i>P</i> = 0.033) and statistically highly significant between Group A and Group B, and Group B and Group C specimens (<i>P</i> < 0.001) on <i>post hoc</i> analysis by Mann-Whitney <i>U</i>-test for pairwise comparisons among independent groups.</p><p><strong>Conclusion: </strong>Based on the findings obtained, it could be concluded that the median retention strength measured was highest for Group C specimens (9.045 MPa), followed by Group A specimens (8.365 MPa), and least for Group B specimens (5.36 MPa).</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"14 1","pages":"21-29"},"PeriodicalIF":0.0,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147773897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impaired Glucose Homeostasis Accompanies Cellular Changes in Endocrine Pancreas after Atorvastatin Administration. 服用阿托伐他汀后内分泌胰腺细胞变化伴随着葡萄糖稳态受损
Journal of Microscopy and Ultrastructure Pub Date : 2024-06-20 eCollection Date: 2024-07-01 DOI: 10.4103/jmau.jmau_41_21
Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany
{"title":"Impaired Glucose Homeostasis Accompanies Cellular Changes in Endocrine Pancreas after Atorvastatin Administration.","authors":"Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany","doi":"10.4103/jmau.jmau_41_21","DOIUrl":"10.4103/jmau.jmau_41_21","url":null,"abstract":"<p><p>Atorvastatin (ATOR) has been reported to increase the risk for diabetes mellitus. Therefore, in the current study, we focused on studying the effect of ATOR on the structure of islets of Langerhans including their various cellular components as well as on glucose homeostasis. We detected a statistically significant increase (<i>P</i> < 0.05) in β-cell mass and percentage with a significant decrease in α-cell area and percentage in animals that received ATOR compared to control ones. In addition, a statistically significant increase (<i>P</i> < 0.05) in the β-cell proliferation was observed in the ATOR group with negligible change in expression of inflammatory cytokines of the islets. A significant downregulation in apoptosis alongside a significant upregulation in anti-apoptosis were detected in islets of animals treated with ATOR. Moreover, there was a significant impairment in various parameters of glucose homeostasis in the ATOR-treated group. Therefore, ATOR may induce insulin resistance-like state that was demarcated at cellular as well as at biochemical levels with little or no inflammatory response.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"12 3","pages":"126-133"},"PeriodicalIF":0.0,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Red Blood Cells in Health and Disease. 红细胞在健康和疾病。
Journal of Microscopy and Ultrastructure Pub Date : 2024-05-07 eCollection Date: 2025-07-01 DOI: 10.4103/jmau.jmau_70_23
Aradhya Giri, Sandhya Tamgadge
{"title":"Red Blood Cells in Health and Disease.","authors":"Aradhya Giri, Sandhya Tamgadge","doi":"10.4103/jmau.jmau_70_23","DOIUrl":"10.4103/jmau.jmau_70_23","url":null,"abstract":"<p><p>Red blood cells (RBCs) play a crucial role in the normal functioning of the human body, primarily through their ability to transport oxygen and carbon dioxide. Various diseases, including anemia and other hemolytic disorders, can arise when there is an abnormality in RBC structure or function. The pathophysiology of other conditions, such as cancer and cardiovascular disease can also involve changes in RBCs. Advances in RBC research have led to a better understanding of their structure, function, and pathophysiology. The COVID-19 pandemic has also highlighted the critical role of RBCs in disease pathology, with research suggesting that RBCs may be directly affected by the virus. This review provides a comprehensive overview of the current state of RBCs in health and disease, including recent advances in diagnosis, treatment, and the role of RBCs in disease pathology.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"13 3","pages":"130-136"},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499939/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Efficacy of Isoamyl 2-Cyanoacrylate Over Facial Lacerations and Surgical Incisions: A Prospective Clinical Study. 2-氰基丙烯酸酯异戊酯治疗面部撕裂伤和外科切口的疗效:一项前瞻性临床研究。
Journal of Microscopy and Ultrastructure Pub Date : 2024-04-10 eCollection Date: 2025-10-01 DOI: 10.4103/jmau.jmau_182_23
Santhosh Panuganti, Ajay Chandran, Kurian Punnoose, Faleh Alshahrani, Abishek R Balaji, Abdulsalam S Alshammari, Suresh Babu Jandrajupalli, Swarnalatha Chandolu, Abhishek Singh Nayyar
{"title":"Efficacy of Isoamyl 2-Cyanoacrylate Over Facial Lacerations and Surgical Incisions: A Prospective Clinical Study.","authors":"Santhosh Panuganti, Ajay Chandran, Kurian Punnoose, Faleh Alshahrani, Abishek R Balaji, Abdulsalam S Alshammari, Suresh Babu Jandrajupalli, Swarnalatha Chandolu, Abhishek Singh Nayyar","doi":"10.4103/jmau.jmau_182_23","DOIUrl":"10.4103/jmau.jmau_182_23","url":null,"abstract":"<p><strong>Objective: </strong>Technological advancements in the new era have introduced a plethora of new methods, as well as synthetic, semi-synthetic, and natural (bio) materials with improved properties for wound closure. The aim of the present study was to evaluate the efficacy of isoamyl 2-cyanoacrylate for wound closure over facial lacerations and surgical incisions compared to conventional methods.</p><p><strong>Materials and methods: </strong>The present prospective clinical study comprised two groups, including group I (incision) and group II (laceration), with 20 patients in each group in whom isoamyl 2-cyanoacrylate was used in the management of facial lacerations and surgical incisions. Various parameters related to wound healing, including the time taken to perform the procedure, step-off borders, contour irregularity, edge inversion, wound margins, fibrous tissue growth and scarring, and the total duration of healing, were assessed and compared over a period of time. The data obtained were subjected to statistical analysis.</p><p><strong>Results: </strong>The present study revealed excellent overall cosmetic results with the use of isoamyl 2-cyanoacrylate. However, the results achieved were more surprising in group I (incision) than in group II (laceration), with a kind of complete satisfaction experienced by the patients as well as the operating surgeon.</p><p><strong>Conclusions: </strong>Based on the results obtained in the present study, it can be concluded that isoamyl 2-cyanoacrylate can serve as a better wound closure material compared to the standard method of incision closure with the help of sutures over the facial tissues.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"13 4","pages":"186-192"},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12710725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145781177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fully Automated GeneXpert BCR-ABL1 Assay versus Standard Nonautomated BCR-ABL1 Real-Time Reverse Transcription-Polymerase Chain Reaction Quantitation: A Study from Tertiary Care Institute in India. 全自动GeneXpert BCR-ABL1测定与标准非自动化BCR-ABL1实时逆转录聚合酶链反应定量:印度三级保健研究所的一项研究。
Journal of Microscopy and Ultrastructure Pub Date : 2024-04-10 eCollection Date: 2025-07-01 DOI: 10.4103/jmau.jmau_88_23
Shilpi More, Tathagata Chatterjee, Charu Agarwal, Mukta Pujani, Raj K Chandoke
{"title":"Fully Automated GeneXpert BCR-ABL1 Assay versus Standard Nonautomated BCR-ABL1 Real-Time Reverse Transcription-Polymerase Chain Reaction Quantitation: A Study from Tertiary Care Institute in India.","authors":"Shilpi More, Tathagata Chatterjee, Charu Agarwal, Mukta Pujani, Raj K Chandoke","doi":"10.4103/jmau.jmau_88_23","DOIUrl":"10.4103/jmau.jmau_88_23","url":null,"abstract":"<p><strong>Background: </strong>Molecular monitoring of chronic myeloid leukemia (CML) patients receiving tyrosine kinase inhibitors at various time points after diagnosis is indispensable for therapeutic stratification. Thus, standardization and reproducibility of these tests to an international scale are of paramount importance. Standard TRUPCR BCR-ABL1 by quantitative real-time reverse transcription-polymerase chain reaction and Xpert BCR-ABL Ultra performed on the Cepheid GeneXpert<sup>®</sup> Instrument Systems (GeneXpert) were used to perform molecular monitoring in CML patients.</p><p><strong>Materials and methods: </strong>Twenty-six samples (6 diagnostic and 20 monitoring samples) were tested on both platforms, and the BCR-ABL1 transcript ratio was compared. Kappa statistic was applied to calculate the agreement between the two tests, and Chi-square test was used to evaluate the difference in proportions in the two groups. The costs between the two tests were also compared, however, no statistical analysis was done for the same.</p><p><strong>Results: </strong>All six diagnostic samples were detected by TRUPCR, but only four could be detected by GeneXpert. One of these was a minor BCR-ABL1 transcript (p190) which cannot be detected by GeneXpert. Among the 20 monitoring samples, there was moderate agreement between the two tests, with a <i>P</i> = 0.01. The cost of TRUPCR was significantly less compared to GeneXpert.</p><p><strong>Conclusion: </strong>Standard BCR-ABL1 estimation by TRUPCR is superior and much more cost-effective than GeneXpert. GeneXpert inspite being an easy-to-use, fully automated platform lacks the benefit of customization with a high cost making the standard <i>reverse transcription</i>-<i>polymerase chain reaction</i> techniques more feasible and usable for molecular monitoring in CML patients.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"13 3","pages":"137-141"},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499937/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of Electromagnetic Radiation of Wi-Fi Router on Thyroid Gland and the Possible Protective Role of Combined Vitamin C and Zinc Administration in Adult Male Albino Rats. Wi-Fi 路由器的电磁辐射对成年雄性白化大鼠甲状腺的影响以及联合服用维生素 C 和锌可能起到的保护作用
Journal of Microscopy and Ultrastructure Pub Date : 2024-04-10 eCollection Date: 2024-04-01 DOI: 10.4103/jmau.jmau_121_23
Nancy Nagy Abd El-Hady Ibrahim, Remon S Estfanous, Aml M Abo-Alala, Amal K Elkattan, Rabab Mohamed Amer
{"title":"Effect of Electromagnetic Radiation of Wi-Fi Router on Thyroid Gland and the Possible Protective Role of Combined Vitamin C and Zinc Administration in Adult Male Albino Rats.","authors":"Nancy Nagy Abd El-Hady Ibrahim, Remon S Estfanous, Aml M Abo-Alala, Amal K Elkattan, Rabab Mohamed Amer","doi":"10.4103/jmau.jmau_121_23","DOIUrl":"10.4103/jmau.jmau_121_23","url":null,"abstract":"<p><strong>Introduction: </strong>Electromagnetic radiation (EMR) is widely used nowadays in various fields due to rapid expansion of technology and affects different organs such as endocrine glands. Antioxidants protect the cells and act as a free radical scavenger.</p><p><strong>Aim of work: </strong>The aim of the study was to clarify the effect of EMR emitted from Wi-Fi router on the thyroid gland of adult male albino rats and the possible protective role of combined Vitamin C and zinc.</p><p><strong>Materials and methods: </strong>Thirty adult male albino rats were divided into three groups: Group I (control group), Group II (received combined Vitamin C and Zinc in one tablet called IMMUNO-MASH), and Group III (experimental groups). Group III was divided into two subgroups (A and B) according to the duration of exposure: 6 h and 24 h/day. Each of these groups was divided into two equal subgroups. One was exposed only to EMR while the other was exposed to EMR and received combined Vitamin C and zinc. All rats were weighed at the beginning and at the end of the experiment. The thyroid gland was prepared for general histological, anti-calcitonin immunostaining, and ultrastructural study. Furthermore, measurement of total serum T3, T4, and thyroid-stimulating hormone (TSH) hormone levels and quantitative analysis of immunoreactive C-cells were done. Then, statistical analysis was done on the number of immunoreactive C-cells, data of the body weight, and the hormonal levels.</p><p><strong>Results: </strong>A highly significant increase in the body weight in subgroups exposed to EMR for 24 h/day was observed. Furthermore, they showed a highly significant decline in T3 and T4 levels together with a highly significant increase in TSH level. With increasing period of exposure, there was a variable degree of deterioration in the form of congestion and dilatation of blood vessels, cellular infiltration, follicular disintegration, vacuolar degeneration, and desquamated follicular cells in the colloid. The C-cells showed a significant increase in the mean number compared with the control group. Ultrastructural analysis of follicular cells revealed colloid droplets, deteriorations in rough endoplasmic reticulum, degenerating nuclei, and swollen mitochondria according to the dose of exposure. There was apparent improvement with the use of combined Vitamin C and zinc.</p><p><strong>Conclusion: </strong>Wi-Fi radiation has a very serious effect on thyroid gland morphology and activity. Moreover, experimentally induced hypothyroidism by radiation resulted in increased C-cell number. Combined Vitamin C and zinc could have a protective role against this tissue damage.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"12 2","pages":"51-61"},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11245130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Cauliflower-like Growth on the Anterior Mandibular Gingivae 下颌前牙龈上长出菜花状赘生物
Journal of Microscopy and Ultrastructure Pub Date : 2024-02-21 DOI: 10.4103/jmau.jmau_54_23
Subraj J. Shetty, T. Pereira, R. Desai
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