Journal of Microscopy and Ultrastructure最新文献

{"title":"Comparative Evaluation of the Osteogenic Potential of Titanium Discs Subjected to Argon Plasma Surface Treatment: An in vitro Study","authors":"Poonam M. Sardesai, Ramesh P. Nayakar, Ritiha C. Uppin, Sayed M. Killedar, Mangesh A. Kakodker","doi":"10.4103/jmau.jmau_138_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_138_23","url":null,"abstract":"\u0000 \u0000 \u0000 Titanium is the most widely used material for dental implants; however, its biological aging can lead to a decreased rate of osseointegration. Titanium surfaces on exposure to argon plasma possess a hydrophilic surface that increases the biological activity of osteoblasts on the implant surface. Hence, this in vitro study was undertaken to assess and compare the osteogenic potential and proliferative nature of osteoblast-like cells on titanium when subjected to argon plasma surface treatment.\u0000 \u0000 \u0000 \u0000 A total of 108 titanium discs (10 mm × 2 mm ASTM B348) were included in the study, and their surface topography was characterized. The test specimens were divided into two subgroups based on surface treatments used, i.e. the study group (n = 54): titanium discs treated with argon plasma and the control group (n = 54): sandblasted titanium discs. The osteogenic potential of the specimens was evaluated by assessing the cell attachment using a hemocytometer and cell proliferation using an MTT assay on MG-63 cell lines at three different time intervals of 24, 48, and 72 h.\u0000 \u0000 \u0000 \u0000 The cell attachment and cell proliferation values were statistically significant (P = 0.001*). In comparison to the control group, these two parameters were considerably greater in the plasma-treated group. The total effect size of the study group was 85% as opposed to 35% of the control group.\u0000 \u0000 \u0000 \u0000 Argon plasma surface treatment had a positive effect on the cellular events of MG-63 cells which can be thought of as an added advantage along with the decontamination procedure for titanium to help in the process of osseointegration.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140443316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Fibroblast Activation Protein-alpha in Odontogenic Lesions – A Retrospective Immunohistochemical Study","authors":"Sandhya Tamgadge, T. Pereira","doi":"10.4103/jmau.jmau_127_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_127_23","url":null,"abstract":"\u0000 \u0000 \u0000 The tumor microenvironment (TME) includes cellular and noncellular components that play important roles in tumor genesis, progression, and therapy response. While much study has been done on the TME in other types of cancer, our understanding of its involvement in odontogenic lesions is still restricted.\u0000 \u0000 \u0000 \u0000 The study group included total (171) odontogenic lesions, which were further divided into two categories. Odontogenic tumors (80) and (2) odontogenic cysts (91). There were 50 cases in the control group. Lymphoma was chosen as the negative control, whereas colorectal and breast carcinomas were chosen as the positive controls. All groups were immunohistochemically stained with the fibroblast activation protein (FAP)-alpha antibody. The samples from the study groups were compared to clinical parameters and statistically evaluated using the Chi-square and Kendall’s tau tests. Unpaired t-test was used to compare the final immune reactivity score (IRS) with the presence or absence of epithelium, radiographic locularity, and ramus involvement. Cronbach’s alpha was used to calculate inter-rater reliability.\u0000 \u0000 \u0000 \u0000 The ameloblastoma tumor group and the odontogenic keratocysts in the cyst group showed a high mean IRS. When the final IRS was compared to a few clinical characteristics such as lesion extension and ramus involvement, showed statistical co-relation.\u0000 \u0000 \u0000 \u0000 With significant connections between the final IRS and a few clinical features, FAP-alpha appears to be a reliable marker for odontogenic lesions. It could be employed as a therapeutic and prognostic marker in future.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"43 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139528045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Benign Phyllodes Tumor with Extensive Cystic Squamous Metaplasia, Clinically Presenting as Malignant Phyllodes","authors":"Mousmi Agrawal, Nighat Hussain, R. Ramchandani, Amit Kumar Chowhan","doi":"10.4103/jmau.jmau_117_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_117_23","url":null,"abstract":"\u0000 Phyllodes tumor are very rare fibroepithelial lesions which comprises 0.3%–1% of all primary breast tumors with peak age around 40–50 years. Phyllodes tumor are classified ino three types: Benign, borderline and malignant tumors. Metaplastic change is very rare in these tumors and can involve both epithelial or stromal components. Stromal metaplastic changes include cartilage, bony or fatty change. Epithelial metaplastic changes include apocrine or squamous change. About 10% of phyllodes tumor show squamous metaplasia. We report a case of 47-year-old patient presenting with large lump in right breast measuring 16 cm × 15 cm × 11 cm, occupying all four quadrants. Clinically, it appeared as malignant phyllodes. But, microscopically, it was benign phyllodes tumor having extensive cystic squamous metaplasia.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"31 44","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139528340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary Oxidative Stress Assessment in Tobacco Users with and without Potentially Malignant Disorders and Micronuclei Estimation using Fluorescent Microscopy","authors":"G. Sudha, Priyadharsini Nataraj, R. Krishnan, A. Rameshkumar","doi":"10.4103/jmau.jmau_73_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_73_23","url":null,"abstract":"\u0000 \u0000 \u0000 Consumption of tobacco in either smoking or nonsmoking form produces free radicals, contributing to an increased risk of oral cancer as a result of oxidative stress. Biochemical changes in antioxidant enzymes are responsible for tobacco-associated oral carcinogenesis. Due to the production of reactive oxygen species and oxidative stress, the promotion of carcinogenesis occurs in the cells. Oral potentially malignant disorders (OPMDs) is an assembly of benign lesions with morphologically altered clinical or histopathological tissue that has a greater than normal risk of transforming into malignant lesions after diagnosis at a later date. Micronuclei are the goal site for early genotoxic events and are involved in carcinogenic events. Micronuclei in exfoliated cells are useful biomarkers in assessing cytogenetic damage. Early detection will help to devise appropriate treatment plans, thereby improving patient survival rates.\u0000 \u0000 \u0000 \u0000 To estimate the salivary superoxide dismutase (SOD) levels and the expression of micronuclei in exfoliated buccal cells of tobacco users before the onset of tobacco-induced oral lesions and correlating the same with patients with OPMDs to determine if this can be useful in predicting early malignant potential in subjects with habitual tobacco usage.\u0000 \u0000 \u0000 \u0000 The study included three groups. Group I – Tobacco users with OPMD (n = 50), Group II – Tobacco users without OPMD (n = 50), and Group III – Healthy controls (n = 50). Sandwich enzyme-linked immunosorbent assay was done for saliva samples for quantification of salivary SOD. Exfoliated buccal cells were smeared and stained with acridine orange stain and viewed under a fluorescent microscope for micronuclei estimation.\u0000 \u0000 \u0000 \u0000 The study showed a significant difference in salivary SOD levels and increased micronuclei expression among tobacco users than healthy controls.\u0000 \u0000 \u0000 \u0000 Our study results revealed a gradual and steady decrease of SOD levels and increased micronuclei expression from healthy control to tobacco users without lesions and tobacco users with lesion, and this can be used as an effective noninvasive early diagnostic biomarker in assessing malignant progression in tobacco users. Furthermore, the evaluation of micronuclei estimation can serve as a simple, quick, and noninvasive chair-side procedure for regular oral cancer screening in patients with tobacco usage.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"31 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odontome – A Single-institutional Cross-sectional Study of 31 Years from Mumbai and Case Report with Modified Gallego’s Stain","authors":"Sandhya Tamgadge, T. Pereira, Rishikesh Mehta, Asma Wagle, A. Tamgadge, K. Bhor","doi":"10.4103/jmau.jmau_66_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_66_23","url":null,"abstract":"\u0000 \u0000 \u0000 Odontomas are the most prevalent type of benign odontogenic tumor. They are made up of several dental hard and soft tissues arranged in diverse patterns. They have slowed growth and normally do not have any symptoms to go along with it. They are not regarded as real neoplasms and are more commonly referred to as hamartomas. There have been very few retrospective studies published in the literature.\u0000 \u0000 \u0000 \u0000 The aim of this study was to examine the clinical findings and histological aspects of 17 odontoma cases as a single-institutional investigation.\u0000 \u0000 \u0000 \u0000 It is a study that was conducted in the past. Data from 17 odontoma cases were collected, then separated, and statistically analyzed based on the number of instances reported, the kind of odontoma, the site of occurrence, age, sex, clinical and histological features, and so on. A brief case report is also provided.\u0000 \u0000 \u0000 \u0000 Of the 17 cases of odontoma reported, 14 were complex odontoma and only 3 were compound odontoma. The age group of 20–29 years had the highest incidence. It was discovered more commonly in the lower jaw than in the maxilla.\u0000 \u0000 \u0000 \u0000 The study indicated a higher prevalence of complex odontomas than compound odontomas in males aged 20–29 years (third decade of life). Early detection and treatment are critical for avoiding disparities.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"51 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139007113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mast Cell Metabolism: A Viable Therapeutic Target against Intraoral Neurofibroma","authors":"Shefali Yadav, DeviCharan Shetty, N. Gulati, A. Jain","doi":"10.4103/jmau.jmau_3_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_3_23","url":null,"abstract":"\u0000 Neurofibroma being benign in its behavior remains silent in existence and hence can be mistaken for various reactive lesions of the oral cavity such as fibroma, peripheral giant cell granuloma, or peripheral ossifying fibroma. Although it is a well-established fact that mast cells play the utmost significance in the tumorigenesis of such tumors, little is known about their importance in treatment modalities. Hence, we intend to present a case of intraoral neurofibroma emphasizing the significance of mast cells in therapeutic intervention of neurofibromas (NFs) as such tumors can be accompanied by syndromes and hence necessitates the thorough clinical as well as microscopical analysis as well as an adequate therapeutic approach. Our case revealed the presence of mast cells in the stroma which was further confirmed with toluidine blue staining. Infiltration of mast cells is frequently seen in NF; however, its presence is uncommon in other neoplasms. This distinctive feature can be taken as a target of interest in neurofibroma research. Current clinical treatment options for NF are limited to surgical removal and follow-up of the case. Hence, this warrants the need of better understanding of the role of mast cell-based targeted therapy for such cases. The strategies formulated against the tumors associated with neurofibroma cannot be only applied after the encounter with sporadic counterparts, specifically in terms of benign tumors linked to such disorders. The noteworthy significance of tumor microenvironment in tumorigenesis necessitates adequate clinical examination, followed by delineation of the differential diagnosis and therapeutic approach toward such cases.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and Biochemical Effects of Plumbagin on Sofosbuvir-induced Renal Cortical Injury in Rats: Role of Tumor Necrosis Factor-Alpha, Interleukin-6, JAK2/STAT3, and Nuclear Factor Kappa B-induced Inflammation","authors":"Samah Kandeel, Eman M. El-Beltagi","doi":"10.4103/jmau.jmau_23_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_23_23","url":null,"abstract":"\u0000 \u0000 \u0000 Hepatitis caused by virus C results in serious health complications. Sofosbuvir is effective for treating hepatitis C but, with side effects especially on kidneys. Plumbagin is a natural plant with a powerful anti-inflammatory effect.\u0000 \u0000 \u0000 \u0000 The assessment of plumbagin effect on the renal cortical damage in rats induced by sofosbuvir, by assessing tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), JAK2/STAT3 and nuclear factor kappa B (NF-κB).\u0000 \u0000 \u0000 \u0000 Forty adult rats (250–300 g) were divided into: group 1 (control); Group 2 received sofosbuvir 36 mg/kg; Group 3 received sofosbuvir and low dose of plumbagin (5 mg/kg); Group 4 received sofosbuvir and mid-dose of plumbagin (10 mg/kg); Group 5 received sofosbuvir and high dose of plumbagin (20 mg/kg); and Group 6 (sofosbuvir recovery). Drugs were taken once daily orally for 8 weeks. Blood samples were collected for the assessment of renal functions and serum TNF-α and IL-6. Renal specimens were processed for both measuring tissue JAK2/STAT3 levels and for histological and immunohistochemical studies.\u0000 \u0000 \u0000 \u0000 Group 2 showed a significant rise of blood urea and serum creatinine, serum TNF-α and IL-6, tissue JAK2/STAT3, hematoxylin and eosin significant histopathological changes, significant increase of collagen area density at Masson’s trichrome and significant rise of NF-κB-positive cells. Plumbagin treated groups showed dose-dependent amelioration of the preceding results. The recovery group showed partial recovery.\u0000 \u0000 \u0000 \u0000 Plumbagin has an ameliorating dose-dependent effect against sofosbuvir-induced renal cortical damage in rats rather than those left to recover alone through its antiinflammatory action. Hence, plumbagin could be promising for the treatment of different inflammatory diseases.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"34 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of Antifungal Property of Acrylic Resin Reinforced with Magnesium Oxide and Silver Nanoparticles and their Effect on Cytotoxic Levels: An In vitro Study","authors":"Meekha Peter, Mahantesh Bembalagi, Hema Kanathila, Varkey Nadakkavukaran Santhosh, Treasa Richa Roy, Mibin Monsy","doi":"10.4103/jmau.jmau_58_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_58_23","url":null,"abstract":"\u0000 \u0000 \u0000 About 65% of people who wear dentures suffer from denture stomatitis, a prevalent pathologic illness that is commonly linked to Candida albicans. The necessity for the creation of polymethyl methacrylate (PMMA) materials with intrinsic activity is brought on by increasing resistance of microbes. The study’s objectives were to examine and compare the antifungal properties of acrylic resin by adding silver nanoparticles (AgNPs) and magnesium oxide nanoparticles (MgONPs), as well as to determine its cellular toxicity at various nanoparticle concentrations on mouse fibroblasts.\u0000 \u0000 \u0000 \u0000 A total of 84 samples were created, including 12 control samples, 36 samples of PMMA resin reinforced with AgNPs, and 36 samples of PMMA resin reinforced with MgONPs. Disk diffusion technique was used to assess the antifungal effectiveness of the samples. By using the MTT test, the cytotoxicity of various nanoparticle concentrations was evaluated. Analysis of variance test and unpaired t-test were used to analyze the data, and then Tukey’s post hoc test was performed.\u0000 \u0000 \u0000 \u0000 The mean values for AgNPs and MgONPs at 2%, 4%, and 6% after 24 h were 13.71 ± 2.07, 18.58 ± 1.62, and 27.96 ± 1.76 and 11.63 ± 1.35, 14.38 ± 1.63, and 18.25 ± 1.39, respectively. In contrast to the MgONPs group, samples with AgNPs showed greater antifungal efficacy against C. albicans. On mouse fibroblasts, MgONP concentrations showed more cytotoxicity, but AgNP concentrations showed less cytotoxicity.\u0000 \u0000 \u0000 \u0000 By adding AgNPs and MgONPs to acrylic resins, which are highly efficient against C. albicans, denture stomatitis can be treated.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"9 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Evaluation of the Shaping Ability of the Recent, Fifth-generation ProTaper Next and Revo-S NiTi Rotary Endodontic Files Using Three-dimensional Imaging: An Imaging-based Study","authors":"Prajna Pattanaik, Akilan Balasubramanian, P. Veeralakshmi, Gautam Singh, Vandana Sadananda, Hina Ahmed, J. Babu, C. Swarnalatha, A. Nayyar","doi":"10.4103/jmau.jmau_82_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_82_23","url":null,"abstract":"\u0000 \u0000 \u0000 The aim of the present study was to assess the shaping ability of the recent, fifth-generation ProTaper Next and Revo-S NiTi rotary endodontic files using three-dimensional imaging.\u0000 \u0000 \u0000 \u0000 For the present in vitro study, 100 freshly extracted mandibular first molars indicated for extraction due to periodontal reasons were collected, while samples were cleaned and the angle of curvature was determined using Weine’s method. The access cavity was prepared using Endo Access Kit, while samples were divided into four groups to be prepared by ProTaper Next and Revo-S rotary endodontic files with or without using Glide path. The canal preparations were done in conjunction with Endo Prep RC, while the shaping ability of the instruments was assessed on the basis of canal transportation and canal-centering ability as assessed from pre- and postoperative cone-beam computed tomography images acquired before and after instrumentation. Statistical analysis was done using the Statistical Package for the Social Sciences (SPSS) version 16.0 (SPSS Inc., Chicago, IL, USA). Kruskal–Wallis H-test was used to compare canal transportation and canal-centering ability of the two file systems among different groups. P <0.05 was considered statistically significant.\u0000 \u0000 \u0000 \u0000 Revo-S files when used with Glide path performed best at 2 mm distance from the apical portion of teeth with zero apical transportation in case of both mesiobuccal (MB) and mesiolingual canals. Furthermore, ProTaper Next files when used with or without Glide path showed higher canal-centering ability as compared to Revo-S files with the mean value being 0.83 ± 0.29 mm in either case when observed at 2 mm distance from the apical portion of teeth in case of MB canals.\u0000 \u0000 \u0000 \u0000 Within the limitations of the present study, the findings obtained in the study suggested that ProTaper Next files had optimal canal-centering ability within the canal as compared to Revo-S files.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"47 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Light and Ultrastructural Analysis in Odontogenic Cysts and Oral Squamous Cell Carcinoma.","authors":"Tanvi Handa, Shally Gupta, Simranjit Singh, Anubha Gulati","doi":"10.4103/jmau.jmau_64_23","DOIUrl":"10.4103/jmau.jmau_64_23","url":null,"abstract":"<p><strong>Background: </strong>The aim of the study was to evaluate the desmosomal and hemidesmosomal attachments in common odontogenic cysts (radicular cyst [RC], odontogenic keratocyst [OKC], and dentigerous cyst [DC]), oral fibroma, and oral squamous cell carcinoma (OSCC). The disruption pattern in OKC was also compared with that of OSCC.</p><p><strong>Materials and methods: </strong>The 125 subjects included in the study were equally divided into five study groups - oral fibroma (Group I - control group), RC (Group II - test group), DC (Group III - test group), OKC (Group IV - test group), and OSCC (Group V - positive control). The clinical diagnosis was confirmed by histopathology, and the disruption was accessed through scanning electron microscopic images.</p><p><strong>Results: </strong>Oral fibroma displayed maximum intact desmosomes and hemidesmosomes and minimal disruption while in OSCC minimum intact desmosomes and hemidesmosomes were evident. Amongst the odontogenic cysts studied, OKC displayed maximum disrupted desmosomes and hemidesmsomes. Further, when OKC and OSCC were compared the completely intact desmosomes and hemidesmosomes were more in OKC than OSCC. The <i>P</i> value was set at <0.05.</p><p><strong>Conclusion: </strong>The study revealed that even though the defect in oral fibroma lies in the connective tissue, trauma or irritation as the etiology likely leads to minimal disruption in these intercellular junctions. These cell junctions were less evident in the case of DC owing to compression of the epithelial lining. The disruption of junctions in radicular cysts was more than those seen in oral fibroma. Compared to the other two cysts, OKCs displayed a much higher proportion of disruption in these cell junctions reflective of their more aggressive clinical behavior. OSCC displayed maximum disruption of cell junctions, which indicated that these disruptions play a role in both carcinogenesis and tumor invasion.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"142-150"},"PeriodicalIF":0.0,"publicationDate":"2023-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12499933/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}