Journal of Microscopy and Ultrastructure最新文献

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Benign Phyllodes Tumor with Extensive Cystic Squamous Metaplasia, Clinically Presenting as Malignant Phyllodes 良性鳞状上皮细胞瘤伴广泛囊性鳞状增生,临床表现为恶性鳞状上皮细胞瘤
Journal of Microscopy and Ultrastructure Pub Date : 2024-01-16 DOI: 10.4103/jmau.jmau_117_23
Mousmi Agrawal, Nighat Hussain, R. Ramchandani, Amit Kumar Chowhan
{"title":"Benign Phyllodes Tumor with Extensive Cystic Squamous Metaplasia, Clinically Presenting as Malignant Phyllodes","authors":"Mousmi Agrawal, Nighat Hussain, R. Ramchandani, Amit Kumar Chowhan","doi":"10.4103/jmau.jmau_117_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_117_23","url":null,"abstract":"\u0000 Phyllodes tumor are very rare fibroepithelial lesions which comprises 0.3%–1% of all primary breast tumors with peak age around 40–50 years. Phyllodes tumor are classified ino three types: Benign, borderline and malignant tumors. Metaplastic change is very rare in these tumors and can involve both epithelial or stromal components. Stromal metaplastic changes include cartilage, bony or fatty change. Epithelial metaplastic changes include apocrine or squamous change. About 10% of phyllodes tumor show squamous metaplasia. We report a case of 47-year-old patient presenting with large lump in right breast measuring 16 cm × 15 cm × 11 cm, occupying all four quadrants. Clinically, it appeared as malignant phyllodes. But, microscopically, it was benign phyllodes tumor having extensive cystic squamous metaplasia.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"31 44","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139528340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salivary Oxidative Stress Assessment in Tobacco Users with and without Potentially Malignant Disorders and Micronuclei Estimation using Fluorescent Microscopy 使用荧光显微镜评估有潜在恶性疾病和无潜在恶性疾病烟草使用者的唾液氧化应激以及微核数量
Journal of Microscopy and Ultrastructure Pub Date : 2023-12-12 DOI: 10.4103/jmau.jmau_73_23
G. Sudha, Priyadharsini Nataraj, R. Krishnan, A. Rameshkumar
{"title":"Salivary Oxidative Stress Assessment in Tobacco Users with and without Potentially Malignant Disorders and Micronuclei Estimation using Fluorescent Microscopy","authors":"G. Sudha, Priyadharsini Nataraj, R. Krishnan, A. Rameshkumar","doi":"10.4103/jmau.jmau_73_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_73_23","url":null,"abstract":"\u0000 \u0000 \u0000 Consumption of tobacco in either smoking or nonsmoking form produces free radicals, contributing to an increased risk of oral cancer as a result of oxidative stress. Biochemical changes in antioxidant enzymes are responsible for tobacco-associated oral carcinogenesis. Due to the production of reactive oxygen species and oxidative stress, the promotion of carcinogenesis occurs in the cells. Oral potentially malignant disorders (OPMDs) is an assembly of benign lesions with morphologically altered clinical or histopathological tissue that has a greater than normal risk of transforming into malignant lesions after diagnosis at a later date. Micronuclei are the goal site for early genotoxic events and are involved in carcinogenic events. Micronuclei in exfoliated cells are useful biomarkers in assessing cytogenetic damage. Early detection will help to devise appropriate treatment plans, thereby improving patient survival rates.\u0000 \u0000 \u0000 \u0000 To estimate the salivary superoxide dismutase (SOD) levels and the expression of micronuclei in exfoliated buccal cells of tobacco users before the onset of tobacco-induced oral lesions and correlating the same with patients with OPMDs to determine if this can be useful in predicting early malignant potential in subjects with habitual tobacco usage.\u0000 \u0000 \u0000 \u0000 The study included three groups. Group I – Tobacco users with OPMD (n = 50), Group II – Tobacco users without OPMD (n = 50), and Group III – Healthy controls (n = 50). Sandwich enzyme-linked immunosorbent assay was done for saliva samples for quantification of salivary SOD. Exfoliated buccal cells were smeared and stained with acridine orange stain and viewed under a fluorescent microscope for micronuclei estimation.\u0000 \u0000 \u0000 \u0000 The study showed a significant difference in salivary SOD levels and increased micronuclei expression among tobacco users than healthy controls.\u0000 \u0000 \u0000 \u0000 Our study results revealed a gradual and steady decrease of SOD levels and increased micronuclei expression from healthy control to tobacco users without lesions and tobacco users with lesion, and this can be used as an effective noninvasive early diagnostic biomarker in assessing malignant progression in tobacco users. Furthermore, the evaluation of micronuclei estimation can serve as a simple, quick, and noninvasive chair-side procedure for regular oral cancer screening in patients with tobacco usage.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"31 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Odontome – A Single-institutional Cross-sectional Study of 31 Years from Mumbai and Case Report with Modified Gallego’s Stain 牙体畸形--孟买 31 年的单机构横断面研究及使用改良加列戈染色法的病例报告
Journal of Microscopy and Ultrastructure Pub Date : 2023-12-12 DOI: 10.4103/jmau.jmau_66_23
Sandhya Tamgadge, T. Pereira, Rishikesh Mehta, Asma Wagle, A. Tamgadge, K. Bhor
{"title":"Odontome – A Single-institutional Cross-sectional Study of 31 Years from Mumbai and Case Report with Modified Gallego’s Stain","authors":"Sandhya Tamgadge, T. Pereira, Rishikesh Mehta, Asma Wagle, A. Tamgadge, K. Bhor","doi":"10.4103/jmau.jmau_66_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_66_23","url":null,"abstract":"\u0000 \u0000 \u0000 Odontomas are the most prevalent type of benign odontogenic tumor. They are made up of several dental hard and soft tissues arranged in diverse patterns. They have slowed growth and normally do not have any symptoms to go along with it. They are not regarded as real neoplasms and are more commonly referred to as hamartomas. There have been very few retrospective studies published in the literature.\u0000 \u0000 \u0000 \u0000 The aim of this study was to examine the clinical findings and histological aspects of 17 odontoma cases as a single-institutional investigation.\u0000 \u0000 \u0000 \u0000 It is a study that was conducted in the past. Data from 17 odontoma cases were collected, then separated, and statistically analyzed based on the number of instances reported, the kind of odontoma, the site of occurrence, age, sex, clinical and histological features, and so on. A brief case report is also provided.\u0000 \u0000 \u0000 \u0000 Of the 17 cases of odontoma reported, 14 were complex odontoma and only 3 were compound odontoma. The age group of 20–29 years had the highest incidence. It was discovered more commonly in the lower jaw than in the maxilla.\u0000 \u0000 \u0000 \u0000 The study indicated a higher prevalence of complex odontomas than compound odontomas in males aged 20–29 years (third decade of life). Early detection and treatment are critical for avoiding disparities.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"51 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139007113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mast Cell Metabolism: A Viable Therapeutic Target against Intraoral Neurofibroma 肥大细胞代谢:口内神经纤维瘤的可行治疗靶点
Journal of Microscopy and Ultrastructure Pub Date : 2023-12-12 DOI: 10.4103/jmau.jmau_3_23
Shefali Yadav, DeviCharan Shetty, N. Gulati, A. Jain
{"title":"Mast Cell Metabolism: A Viable Therapeutic Target against Intraoral Neurofibroma","authors":"Shefali Yadav, DeviCharan Shetty, N. Gulati, A. Jain","doi":"10.4103/jmau.jmau_3_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_3_23","url":null,"abstract":"\u0000 Neurofibroma being benign in its behavior remains silent in existence and hence can be mistaken for various reactive lesions of the oral cavity such as fibroma, peripheral giant cell granuloma, or peripheral ossifying fibroma. Although it is a well-established fact that mast cells play the utmost significance in the tumorigenesis of such tumors, little is known about their importance in treatment modalities. Hence, we intend to present a case of intraoral neurofibroma emphasizing the significance of mast cells in therapeutic intervention of neurofibromas (NFs) as such tumors can be accompanied by syndromes and hence necessitates the thorough clinical as well as microscopical analysis as well as an adequate therapeutic approach. Our case revealed the presence of mast cells in the stroma which was further confirmed with toluidine blue staining. Infiltration of mast cells is frequently seen in NF; however, its presence is uncommon in other neoplasms. This distinctive feature can be taken as a target of interest in neurofibroma research. Current clinical treatment options for NF are limited to surgical removal and follow-up of the case. Hence, this warrants the need of better understanding of the role of mast cell-based targeted therapy for such cases. The strategies formulated against the tumors associated with neurofibroma cannot be only applied after the encounter with sporadic counterparts, specifically in terms of benign tumors linked to such disorders. The noteworthy significance of tumor microenvironment in tumorigenesis necessitates adequate clinical examination, followed by delineation of the differential diagnosis and therapeutic approach toward such cases.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural and Biochemical Effects of Plumbagin on Sofosbuvir-induced Renal Cortical Injury in Rats: Role of Tumor Necrosis Factor-Alpha, Interleukin-6, JAK2/STAT3, and Nuclear Factor Kappa B-induced Inflammation 普鲁卡因对索非布韦诱导的大鼠肾皮质损伤的结构和生化效应:肿瘤坏死因子-α、白细胞介素-6、JAK2/STAT3 和核因子卡巴 B 诱导的炎症的作用
Journal of Microscopy and Ultrastructure Pub Date : 2023-12-12 DOI: 10.4103/jmau.jmau_23_23
Samah Kandeel, Eman M. El-Beltagi
{"title":"Structural and Biochemical Effects of Plumbagin on Sofosbuvir-induced Renal Cortical Injury in Rats: Role of Tumor Necrosis Factor-Alpha, Interleukin-6, JAK2/STAT3, and Nuclear Factor Kappa B-induced Inflammation","authors":"Samah Kandeel, Eman M. El-Beltagi","doi":"10.4103/jmau.jmau_23_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_23_23","url":null,"abstract":"\u0000 \u0000 \u0000 Hepatitis caused by virus C results in serious health complications. Sofosbuvir is effective for treating hepatitis C but, with side effects especially on kidneys. Plumbagin is a natural plant with a powerful anti-inflammatory effect.\u0000 \u0000 \u0000 \u0000 The assessment of plumbagin effect on the renal cortical damage in rats induced by sofosbuvir, by assessing tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), JAK2/STAT3 and nuclear factor kappa B (NF-κB).\u0000 \u0000 \u0000 \u0000 Forty adult rats (250–300 g) were divided into: group 1 (control); Group 2 received sofosbuvir 36 mg/kg; Group 3 received sofosbuvir and low dose of plumbagin (5 mg/kg); Group 4 received sofosbuvir and mid-dose of plumbagin (10 mg/kg); Group 5 received sofosbuvir and high dose of plumbagin (20 mg/kg); and Group 6 (sofosbuvir recovery). Drugs were taken once daily orally for 8 weeks. Blood samples were collected for the assessment of renal functions and serum TNF-α and IL-6. Renal specimens were processed for both measuring tissue JAK2/STAT3 levels and for histological and immunohistochemical studies.\u0000 \u0000 \u0000 \u0000 Group 2 showed a significant rise of blood urea and serum creatinine, serum TNF-α and IL-6, tissue JAK2/STAT3, hematoxylin and eosin significant histopathological changes, significant increase of collagen area density at Masson’s trichrome and significant rise of NF-κB-positive cells. Plumbagin treated groups showed dose-dependent amelioration of the preceding results. The recovery group showed partial recovery.\u0000 \u0000 \u0000 \u0000 Plumbagin has an ameliorating dose-dependent effect against sofosbuvir-induced renal cortical damage in rats rather than those left to recover alone through its antiinflammatory action. Hence, plumbagin could be promising for the treatment of different inflammatory diseases.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"34 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139008973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Evaluation of Antifungal Property of Acrylic Resin Reinforced with Magnesium Oxide and Silver Nanoparticles and their Effect on Cytotoxic Levels: An In vitro Study 氧化镁和纳米银增强丙烯酸树脂抗真菌性能的比较评估及其对细胞毒性水平的影响:体外研究
Journal of Microscopy and Ultrastructure Pub Date : 2023-12-12 DOI: 10.4103/jmau.jmau_58_23
Meekha Peter, Mahantesh Bembalagi, Hema Kanathila, Varkey Nadakkavukaran Santhosh, Treasa Richa Roy, Mibin Monsy
{"title":"Comparative Evaluation of Antifungal Property of Acrylic Resin Reinforced with Magnesium Oxide and Silver Nanoparticles and their Effect on Cytotoxic Levels: An In vitro Study","authors":"Meekha Peter, Mahantesh Bembalagi, Hema Kanathila, Varkey Nadakkavukaran Santhosh, Treasa Richa Roy, Mibin Monsy","doi":"10.4103/jmau.jmau_58_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_58_23","url":null,"abstract":"\u0000 \u0000 \u0000 About 65% of people who wear dentures suffer from denture stomatitis, a prevalent pathologic illness that is commonly linked to Candida albicans. The necessity for the creation of polymethyl methacrylate (PMMA) materials with intrinsic activity is brought on by increasing resistance of microbes. The study’s objectives were to examine and compare the antifungal properties of acrylic resin by adding silver nanoparticles (AgNPs) and magnesium oxide nanoparticles (MgONPs), as well as to determine its cellular toxicity at various nanoparticle concentrations on mouse fibroblasts.\u0000 \u0000 \u0000 \u0000 A total of 84 samples were created, including 12 control samples, 36 samples of PMMA resin reinforced with AgNPs, and 36 samples of PMMA resin reinforced with MgONPs. Disk diffusion technique was used to assess the antifungal effectiveness of the samples. By using the MTT test, the cytotoxicity of various nanoparticle concentrations was evaluated. Analysis of variance test and unpaired t-test were used to analyze the data, and then Tukey’s post hoc test was performed.\u0000 \u0000 \u0000 \u0000 The mean values for AgNPs and MgONPs at 2%, 4%, and 6% after 24 h were 13.71 ± 2.07, 18.58 ± 1.62, and 27.96 ± 1.76 and 11.63 ± 1.35, 14.38 ± 1.63, and 18.25 ± 1.39, respectively. In contrast to the MgONPs group, samples with AgNPs showed greater antifungal efficacy against C. albicans. On mouse fibroblasts, MgONP concentrations showed more cytotoxicity, but AgNP concentrations showed less cytotoxicity.\u0000 \u0000 \u0000 \u0000 By adding AgNPs and MgONPs to acrylic resins, which are highly efficient against C. albicans, denture stomatitis can be treated.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"9 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative Evaluation of the Shaping Ability of the Recent, Fifth-generation ProTaper Next and Revo-S NiTi Rotary Endodontic Files Using Three-dimensional Imaging: An Imaging-based Study 使用三维成像技术比较评估最新第五代 ProTaper Next 和 Revo-S NiTi 旋转根管针的成型能力:基于成像的研究
Journal of Microscopy and Ultrastructure Pub Date : 2023-12-12 DOI: 10.4103/jmau.jmau_82_23
Prajna Pattanaik, Akilan Balasubramanian, P. Veeralakshmi, Gautam Singh, Vandana Sadananda, Hina Ahmed, J. Babu, C. Swarnalatha, A. Nayyar
{"title":"Comparative Evaluation of the Shaping Ability of the Recent, Fifth-generation ProTaper Next and Revo-S NiTi Rotary Endodontic Files Using Three-dimensional Imaging: An Imaging-based Study","authors":"Prajna Pattanaik, Akilan Balasubramanian, P. Veeralakshmi, Gautam Singh, Vandana Sadananda, Hina Ahmed, J. Babu, C. Swarnalatha, A. Nayyar","doi":"10.4103/jmau.jmau_82_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_82_23","url":null,"abstract":"\u0000 \u0000 \u0000 The aim of the present study was to assess the shaping ability of the recent, fifth-generation ProTaper Next and Revo-S NiTi rotary endodontic files using three-dimensional imaging.\u0000 \u0000 \u0000 \u0000 For the present in vitro study, 100 freshly extracted mandibular first molars indicated for extraction due to periodontal reasons were collected, while samples were cleaned and the angle of curvature was determined using Weine’s method. The access cavity was prepared using Endo Access Kit, while samples were divided into four groups to be prepared by ProTaper Next and Revo-S rotary endodontic files with or without using Glide path. The canal preparations were done in conjunction with Endo Prep RC, while the shaping ability of the instruments was assessed on the basis of canal transportation and canal-centering ability as assessed from pre- and postoperative cone-beam computed tomography images acquired before and after instrumentation. Statistical analysis was done using the Statistical Package for the Social Sciences (SPSS) version 16.0 (SPSS Inc., Chicago, IL, USA). Kruskal–Wallis H-test was used to compare canal transportation and canal-centering ability of the two file systems among different groups. P <0.05 was considered statistically significant.\u0000 \u0000 \u0000 \u0000 Revo-S files when used with Glide path performed best at 2 mm distance from the apical portion of teeth with zero apical transportation in case of both mesiobuccal (MB) and mesiolingual canals. Furthermore, ProTaper Next files when used with or without Glide path showed higher canal-centering ability as compared to Revo-S files with the mean value being 0.83 ± 0.29 mm in either case when observed at 2 mm distance from the apical portion of teeth in case of MB canals.\u0000 \u0000 \u0000 \u0000 Within the limitations of the present study, the findings obtained in the study suggested that ProTaper Next files had optimal canal-centering ability within the canal as compared to Revo-S files.\u0000","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"47 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
"Ayoub-Shklar" as a Special Stain for Keratin: A Histopathological Study. 作为角蛋白特殊染色剂的 "Ayoub-Shklar":组织病理学研究。
Journal of Microscopy and Ultrastructure Pub Date : 2023-09-06 eCollection Date: 2024-07-01 DOI: 10.4103/jmau.jmau_34_23
Raman Dineja, Harikrishnan Prasad, KenniyanKumar Sri Chinthu, Muthusamy Rajmohan
{"title":"\"Ayoub-Shklar\" as a Special Stain for Keratin: A Histopathological Study.","authors":"Raman Dineja, Harikrishnan Prasad, KenniyanKumar Sri Chinthu, Muthusamy Rajmohan","doi":"10.4103/jmau.jmau_34_23","DOIUrl":"10.4103/jmau.jmau_34_23","url":null,"abstract":"<p><strong>Background: </strong>Special stains in histopathological studies are used to identify the structures with different dyes apart from the routine stain hematoxylin and Eosin (H and E). The component which is present in the special stains will have a specific bond and affinity for particular tissue components in the histological specimen. Structures like keratin takes up an eosinophilic stain in routine (H and E) staining. Most of the potentially malignant disorders and carcinomas arise due to the keratinization defect, which makes keratin an important diagnostic tool. There are different stains such as Ayoub-Shklar (A-S), Dane-Herman (D-H), and rapid Papanicolaou (PAP) which is used to identify keratin. In A-S stain, keratins can be stained in magenta-red and orange colors.</p><p><strong>Aim: </strong>we compared A-S special stain and routine stain in terms of staining intensity or quality, the pattern of staining, and specificity for staining keratin.</p><p><strong>Materials and methods: </strong>Thirty cases from the department archives that included 10 well-differentiated squamous cell carcinoma, 10 verrucous carcinoma, and 10 epithelial dysplasia were taken and each case was stained with both A-S and H and E stain.</p><p><strong>Results: </strong>A-S showed an almost equal distribution of uniform and patchy staining patterns, but H and E showed more patchy staining patterns in the three groups. H and E stain shows good staining quality than A-S. A-S shows almost 90% of satisfactory staining specificity.</p><p><strong>Conclusion: </strong>Special stain like A-S stain can be used to stain keratin in different color, but H and E always remain gold standard stain.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"109-113"},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphological Spectrum of Bone Marrow Aspirates in Infections: A Clinico-Hematological Analysis. 感染性骨髓穿刺的形态学特征:临床血液学分析。
Journal of Microscopy and Ultrastructure Pub Date : 2023-09-06 eCollection Date: 2024-07-01 DOI: 10.4103/jmau.jmau_20_23
Divya Aggarwal, Shilpi More, Ritika Singh, Meera Sikka, Mrinalini Kotru
{"title":"Morphological Spectrum of Bone Marrow Aspirates in Infections: A Clinico-Hematological Analysis.","authors":"Divya Aggarwal, Shilpi More, Ritika Singh, Meera Sikka, Mrinalini Kotru","doi":"10.4103/jmau.jmau_20_23","DOIUrl":"10.4103/jmau.jmau_20_23","url":null,"abstract":"<p><strong>Context: </strong>Bone marrow examination (BME) is an invaluable tool for cases with pyrexia of unknown origin and pancytopenia. However, it is under-utilized for diagnosing infectious etiology and there is a paucity of literature regarding its role in infective pathology.</p><p><strong>Aims: </strong>This study aims to bring to light the role of BME in diagnosing infectious pathology.</p><p><strong>Settings and design: </strong>A retrospective study was carried out on bone marrow aspirates (BMAs) sent to the hematology department over the past 4 years. Clinical details, peripheral smears and BMA were retrieved from the records and analyzed.</p><p><strong>Subjects and methods: </strong>Leishman-stained peripheral smears and BMA were studied along with bone marrow biopsy wherever feasible.</p><p><strong>Results: </strong>A total of 52 cases were studied. The most common clinical presentation was fever, clinical finding was splenomegaly and hematological finding was anemia. Based on the morphological findings in combination with clinical history, cases were categorized into-parasitic (26.9%), viral (23.1%), tubercular (11.5%), and nonspecific infections (38.5%). Parasites such as <i>Leishmania donovani</i>, microfilaria, plasmodium falciparum, and vivax were reported in 14/52 (27%) cases. Associated BMA findings were plasmacytosis, eosinophilia, reactive lymphocytosis, or dyserythopoiesis. In 38% (20/52) cases, no specific cause of infection was found in the bone marrow. These patients showed histiocytosis, hemophagocytosis, maturation arrest in myeloid lineage, relative myeloid hyperplasia, dysmyelopoiesis, toxic granulation/vacuolation in myeloid cells, lymphocytosis, increased plasma cells or monocytosis in marrow.</p><p><strong>Conclusions: </strong>Increased histiocytes, hemophagocytosis, dysplastic changes, maturation arrest, relative myeloid hyperplasia or reactive plasmacytosis, lymphocytosis, and monocytosis are BMA features which must alert the pathologist towards an infectious disease process, a knowledge of these changes can help extend the scope of BME beyond hemato-lymphoid malignancies.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"114-119"},"PeriodicalIF":0.0,"publicationDate":"2023-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11537354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dysplastic lining in odontogenic keratocyst: A unique and unanswered feature 牙源性角化囊肿的内衬不全:一个独特且未回答的特征
Journal of Microscopy and Ultrastructure Pub Date : 2023-07-01 DOI: 10.4103/jmau.jmau_120_20
H. Likhithaswamy, Kokila Ganganna, K. A. Ashok Kumar, Mahalakshmi Saibaba
{"title":"Dysplastic lining in odontogenic keratocyst: A unique and unanswered feature","authors":"H. Likhithaswamy, Kokila Ganganna, K. A. Ashok Kumar, Mahalakshmi Saibaba","doi":"10.4103/jmau.jmau_120_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_120_20","url":null,"abstract":"Odontogenic keratocyst (OKC) is one of the most controversial and extensively studied and discussed odontogenic cyst among oral pathologists and surgeons due to its aggressive behavior and high recurrence rate. Recurrence of OKC is attributed to clinical aspects per se, surgical skill and treatment option or histopathological reasons have been extensively discussed in various literature. Although malignant transformation in OKC has been reported, little is known regarding dysplasia alone in OKC epithelium. In this article, we have highlighted this histological feature which can give a hint in predicting malignant transformation. This may help the clinician to predict the prognosis of the disease and thereby improving the quality of life.","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"193 - 195"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46891777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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