槲皮素靶向氧化应激、自噬和细胞凋亡改善大鼠顺铂诱导的周围神经病变。

Q3 Medicine
Heba A Mahmoud, Hemat E El Horany, Marwa Aboalsoud, Rania Nagi Abd-Ellatif, Amal Ahmed El Sheikh, Alshimaa Aboalsoud
{"title":"槲皮素靶向氧化应激、自噬和细胞凋亡改善大鼠顺铂诱导的周围神经病变。","authors":"Heba A Mahmoud,&nbsp;Hemat E El Horany,&nbsp;Marwa Aboalsoud,&nbsp;Rania Nagi Abd-Ellatif,&nbsp;Amal Ahmed El Sheikh,&nbsp;Alshimaa Aboalsoud","doi":"10.4103/jmau.jmau_78_22","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"107-114"},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf","citationCount":"0","resultStr":"{\"title\":\"Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.\",\"authors\":\"Heba A Mahmoud,&nbsp;Hemat E El Horany,&nbsp;Marwa Aboalsoud,&nbsp;Rania Nagi Abd-Ellatif,&nbsp;Amal Ahmed El Sheikh,&nbsp;Alshimaa Aboalsoud\",\"doi\":\"10.4103/jmau.jmau_78_22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>\",\"PeriodicalId\":16340,\"journal\":{\"name\":\"Journal of Microscopy and Ultrastructure\",\"volume\":\"11 2\",\"pages\":\"107-114\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Microscopy and Ultrastructure\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jmau.jmau_78_22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microscopy and Ultrastructure","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jmau.jmau_78_22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

摘要

背景:槲皮素是一种类黄酮,具有抗氧化和自噬调节活性。顺铂是铂基抗癌药物之一。周围神经病变的早期发展作为顺铂的不良反应干扰治疗的继续。氧化应激和自噬损伤可能起作用。目的:探讨槲皮素对顺铂所致周围神经病变的保护作用。方法:24只雄性Wistar大鼠分为3组:1组(对照组)和2组(顺铂组),采用单趾注射顺铂诱导周围神经病变。组3(顺铂+槲皮素组)给予顺铂单次静脉注射,再用槲皮素治疗14 d。实验结束后,采用尾浸试验评价伤害感受,然后采血进行神经生长因子分析。应用免疫组化染色观察坐骨神经组织病理学变化及轻链3-II。测定坐骨神经组织匀浆中还原性谷胱甘肽、丙二醛、mTOR和caspase-3的含量。结果:槲皮素可显著改善顺铂诱导的伤害性损伤,减轻顺铂诱导的氧化应激、自噬和细胞凋亡,保护神经元免于死亡。结论:从目前的研究来看,槲皮素可以作为顺铂诱导的周围神经病变的一种有前景的保护剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.

Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.

Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.

Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.

Background: Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.

Aim: This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.

Methods: Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.

Results: This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.

Conclusion: From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
1.90
自引率
0.00%
发文量
46
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信