Heba A Mahmoud, Hemat E El Horany, Marwa Aboalsoud, Rania Nagi Abd-Ellatif, Amal Ahmed El Sheikh, Alshimaa Aboalsoud
{"title":"槲皮素靶向氧化应激、自噬和细胞凋亡改善大鼠顺铂诱导的周围神经病变。","authors":"Heba A Mahmoud, Hemat E El Horany, Marwa Aboalsoud, Rania Nagi Abd-Ellatif, Amal Ahmed El Sheikh, Alshimaa Aboalsoud","doi":"10.4103/jmau.jmau_78_22","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 2","pages":"107-114"},"PeriodicalIF":0.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf","citationCount":"0","resultStr":"{\"title\":\"Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.\",\"authors\":\"Heba A Mahmoud, Hemat E El Horany, Marwa Aboalsoud, Rania Nagi Abd-Ellatif, Amal Ahmed El Sheikh, Alshimaa Aboalsoud\",\"doi\":\"10.4103/jmau.jmau_78_22\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.</p><p><strong>Aim: </strong>This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.</p><p><strong>Methods: </strong>Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.</p><p><strong>Results: </strong>This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.</p><p><strong>Conclusion: </strong>From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.</p>\",\"PeriodicalId\":16340,\"journal\":{\"name\":\"Journal of Microscopy and Ultrastructure\",\"volume\":\"11 2\",\"pages\":\"107-114\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/7b/eb/JMAU-11-107.PMC10337675.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Microscopy and Ultrastructure\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jmau.jmau_78_22\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Microscopy and Ultrastructure","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jmau.jmau_78_22","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Targeting Oxidative Stress, Autophagy, and Apoptosis by Quercetin to Ameliorate Cisplatin-induced Peripheral Neuropathy in Rats.
Background: Quercetin is a flavonoid, with antioxidant and autophagy-modulating activities. Cisplatin is one of the platinum-based anticancer drugs. Early development of peripheral neuropathy as an adverse effect of cisplatin interferes with the continuation of therapy. Oxidative stress and autophagy impairment may play a role.
Aim: This study aimed to explore the possible protective effects of quercetin against cisplatin-induced peripheral neuropathy.
Methods: Twenty-four male Wistar rats were divided into three groups: Group 1 (control group) and Group 2 (cisplatin group) where peripheral neuropathy was induced using single ip injection of cisplatin. Group 3 (cisplatin + quercetin group) received single ip injection of cisplatin and was then treated with quercetin for 14 days. At the end of the experiment, nociception was evaluated by tail immersion test, and then, blood was collected for analysis of nerve growth factor. Sciatic nerve was used to assess histopathological changes and light chain 3-II by immunohistochemical staining. Reduced glutathione, malondialdehyde, mTOR, and caspase-3 were estimated in sciatic nerve tissue homogenate.
Results: This research work revealed that quercetin significantly improved cisplatin-induced nociceptive impairment, attenuated cisplatin-induced oxidative stress, autophagy, and apoptosis to protect against neuronal death.
Conclusion: From the current study, quercetin can act as a promising protective agent against cisplatin-induced peripheral neuropathy.