Journal of Microscopy and Ultrastructure最新文献

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Odontoma - Basic Third Dimensional Histopathology Model Using 3D Animation Software and Clay Model. 牙瘤-基本三维组织病理学模型使用三维动画软件和粘土模型。
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-05 eCollection Date: 2024-10-01 DOI: 10.4103/jmau.jmau_37_22
Sandhya Tamgadge, Avinash P Tamgadge, Amisha Nayak
{"title":"Odontoma - Basic Third Dimensional Histopathology Model Using 3D Animation Software and Clay Model.","authors":"Sandhya Tamgadge, Avinash P Tamgadge, Amisha Nayak","doi":"10.4103/jmau.jmau_37_22","DOIUrl":"10.4103/jmau.jmau_37_22","url":null,"abstract":"<p><strong>Background: </strong>Oral lesions of the jaw has been well explained in the literature through high-quality 2D clinical, radiographic, and histopathological images. But the clinicians and most of the times students too, don't understand the histopathological aspect through the description of text and existing 2D images. This article is preliminary attempt to explain the key events of histopathological aspects of odontoma in third dimension and clay models in a life-like manner which author herself has designed, for better understanding not only by oral and general pathologists, and students but also by patients, which is the unique feature of this short manuscript.</p><p><strong>Aim: </strong>To create 3D animation video and images of odontoma using various 3D animation softwares and clay models.</p><p><strong>Methodology: </strong>Preliminary 3D images and videos on histopathological aspect of odontoma were designed by using 3Dmax (Autodesk Media and Entertainment) and Adobe premiere pro 5.5 software which is a video editing software (Adobe Systems). Additionally models were prepared using kids' colourful clay material.</p><p><strong>Observation: </strong>The clay model and 3D animation videos gave life like picture of a disease.</p><p><strong>Conclusion: </strong>3D animation and clay models to explain histopathology has promising future for histology and histopathology.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":"211-213"},"PeriodicalIF":0.0,"publicationDate":"2023-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11729023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of the Hair Cells of the Human Cochlea: A Scanning Electron Microscopic Study. 人耳蜗毛细胞的发育:扫描电镜研究。
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/JMAU.JMAU_107_20
Sabita Mishra, Tarasankar Roy, Shubhi Saini
{"title":"Development of the Hair Cells of the Human Cochlea: A Scanning Electron Microscopic Study.","authors":"Sabita Mishra,&nbsp;Tarasankar Roy,&nbsp;Shubhi Saini","doi":"10.4103/JMAU.JMAU_107_20","DOIUrl":"https://doi.org/10.4103/JMAU.JMAU_107_20","url":null,"abstract":"<p><strong>Introduction: </strong>In the mammalian auditory system, the cochlea is the first to attain structural and functional maturity. Although ultrastructural details of the developing cochlea of lower animals have been elucidated in the last few decades, comprehensive studies on human cochlea are lacking.</p><p><strong>Materials and methods: </strong>In the present investigation we studied the development and maturation of the hair cells of ten human fetal cochlea from gestational weeks (GW) 12 to 37 by scanning electron microscopy.</p><p><strong>Result: </strong>We observed undifferentiated hair cells possessing numerous surface projections and long kinocilium during GW 14. At GW16, the primitive hair cells were arranged in one inner and four outer rows and had globular apices indicating the initiation of stereocilia formation. By GW 22, the globular apices were replaced by linear stereocilia and occasional kinocillia. Mature hair cells with sterocilia were observed in the basal turn at 30th week of gestation. At GW 37, the stereocilia were arranged in a typical \"V\" shaped pattern at the middle and apical coil, while the stereocilia of the basal turn were shorter in length resembling the adult cochlea. The inner hair cells were long and slender while outer hair cells were pear shaped, kinocilium were absent and the tunnel of Corti were well formed.</p><p><strong>Conclusion: </strong>It is concluded that in human, the morphological maturation of the hair cells starts in the basal turn around GW 22 and continues till 37th week in the apical turn indicating that early maturation of the cochlea may have a role on development of the higher auditory pathway connections.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/1e/JMAU-11-17.PMC10153736.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9415553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pioglitazone Induces Dysplastic Urothelial Changes in Urinary Bladder of Experimental Diabetes. 吡格列酮诱导实验性糖尿病患者膀胱尿路上皮发育不良。
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_34_21
Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany
{"title":"Pioglitazone Induces Dysplastic Urothelial Changes in Urinary Bladder of Experimental Diabetes.","authors":"Ahmed A M Abdel-Hamid,&nbsp;Alaa El-Din L Firgany","doi":"10.4103/jmau.jmau_34_21","DOIUrl":"https://doi.org/10.4103/jmau.jmau_34_21","url":null,"abstract":"<p><strong>Objectives: </strong>Pioglitazone (PIO) is a widely prescribed oral antidiabetic drug that has concerns regarding a potential risk of developing carcinoma of the urinary bladder. The objective of the current study was to assess this potential risk.</p><p><strong>Materials and methods: </strong>The potential risk of PIO-induced urinary bladder carcinoma was assessed in the current study by examining urinary bladder of rats for urothelial cytokeratin (CK) expression and proliferative activity by Ki67 immunostaining.</p><p><strong>Results: </strong>Histological examination revealed dysplastic urothelial changes in PIO <i>per se</i> and diabetes mellitus + PIO (diabetic rats receiving PIO). In addition, a significantly (<i>P</i> < 0.05) decreased CK7 and CK8 expression together with a significantly increased CK20 as well as Ki67 expression was detected in the urothelial cells of groups administrated PIO, contrary to those which did not.</p><p><strong>Conclusion: </strong>The manifestations of urothelial dysplasia evidenced by histological examination as well as by the aberrant expression in CK and Ki67 after PIO administration add supporting evidence at cellular and experimental level to the previous clinical suspicions.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"34-40"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/62/c5/JMAU-11-34.PMC10153741.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9415554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Seasonal changes in the localization of cytokeratin AE1, alpha-smooth muscle actin, and S100 proteins in the testis and epididymis of the African Fruit Bat (Epomops franqueti 非洲果蝠睾丸和附睾细胞角蛋白AE1、α -平滑肌肌动蛋白和S100蛋白定位的季节变化
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_121_22
O. Ekeolu, S. Olukole, B. Oke
{"title":"Seasonal changes in the localization of cytokeratin AE1, alpha-smooth muscle actin, and S100 proteins in the testis and epididymis of the African Fruit Bat (Epomops franqueti","authors":"O. Ekeolu, S. Olukole, B. Oke","doi":"10.4103/jmau.jmau_121_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_121_22","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of antimicrobial efficacy and osteogenic potential of titanium coated with hydrogel of Calendula officinalis: An in Vitro Study 金盏菊水凝胶包覆钛的体外抗菌效果及成骨潜力评价
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_22_23
P. Sardesai, Ramesh P. Nayakar, Ritiha Uppin, Sayed M. Killedar, K. Sutar, Anandkumar G. Patil
{"title":"Evaluation of antimicrobial efficacy and osteogenic potential of titanium coated with hydrogel of Calendula officinalis: An in Vitro Study","authors":"P. Sardesai, Ramesh P. Nayakar, Ritiha Uppin, Sayed M. Killedar, K. Sutar, Anandkumar G. Patil","doi":"10.4103/jmau.jmau_22_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_22_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pleomorphic adenoma in an immunocompromised patient 免疫功能低下患者的多形性腺瘤
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_90_22
C. Babu, Sandhya Tamgadge, T. Pereira, Vishal Punjabi
{"title":"Pleomorphic adenoma in an immunocompromised patient","authors":"C. Babu, Sandhya Tamgadge, T. Pereira, Vishal Punjabi","doi":"10.4103/jmau.jmau_90_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_90_22","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Comparative Evaluation of Efficacy of BioMin F and Propolis Containing Toothpastes on Dentinal Tubule Occlusion with and Without Use of an Adjunct 810 nm Diode Laser: An In vitro Scanning Electron Microscope Study. 生物素F与蜂胶牙膏治疗牙本质小管闭塞的体外扫描电镜对比研究
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_133_20
Komal Khare, Prashant Bhusari, Abhishek Soni, Sachin K Malagi, Dennis Abraham, Lynn Johnson
{"title":"A Comparative Evaluation of Efficacy of BioMin F and Propolis Containing Toothpastes on Dentinal Tubule Occlusion with and Without Use of an Adjunct 810 nm Diode Laser: An <i>In vitro</i> Scanning Electron Microscope Study.","authors":"Komal Khare,&nbsp;Prashant Bhusari,&nbsp;Abhishek Soni,&nbsp;Sachin K Malagi,&nbsp;Dennis Abraham,&nbsp;Lynn Johnson","doi":"10.4103/jmau.jmau_133_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_133_20","url":null,"abstract":"<p><strong>Introduction: </strong>Dentinal hypersensitivity (DH) is a common chronic condition that affects a high proportion of the adult population and is one of the main reasons why patients seek dental opinion and treatment. Despite considerable success in reducing DH, unfortunately, most of the present methods can provide only temporary and unpredictable desensitization. Therefore, new treatment methods that are effective for long periods and at the same time do not have the complications are needed. Recently, two new desensitizing agents BioMin F and Propolis have been introduced for the treatment of DH.</p><p><strong>Aim: </strong>The aim of the study was to compare and evaluate the efficacy of BioMin F and Propolis containing toothpastes on dentinal tubule occlusion with and without the use of an adjunct 810 nm Diode Laser.</p><p><strong>Materials and methods: </strong>Forty-five freshly extracted teeth were taken out of which thirty were sectioned into halves and divided into four test groups BioMin F, Propolis, BioMin F + Laser, and Propolis + Laser and control group. All the specimens were treated twice a day for 7 days and then evaluated under scanning electron microscope for partial and complete dentinal tubule occlusion.</p><p><strong>Results: </strong>A significantly higher number of completely occluded tubules were seen in BioMin F + laser group followed by Propolis + laser, Biomin F, and Propolis.</p><p><strong>Conclusion: </strong>Combination approach of desensitizing agent and laser provided a better result than the desensitizing agent alone and when compared individually Biomin F was more effective desensitizer as compared to Propolis.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/27/JMAU-11-41.PMC10153734.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Inappropriate oral habits and mucocele in children 儿童不适当的口腔习惯和粘液囊肿
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_18_23
Nandhini Gunasekaran, Dineshkumar Thayalan, A. James, A. Vignesh
{"title":"Inappropriate oral habits and mucocele in children","authors":"Nandhini Gunasekaran, Dineshkumar Thayalan, A. James, A. Vignesh","doi":"10.4103/jmau.jmau_18_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_18_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Comparison of Proliferative Capacity of Reticular and Erosive Variants of Oral Lichen Planus by Argyrophilic Nucleolar Organizer Regions Method. 用嗜银核仁组织区法比较口腔扁平苔藓网状型和侵蚀型的增殖能力。
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_104_20
Swati Shrikant Gotmare, Anish A Gupta, Mandavi Waghmare, Pratibha Kavle, Asha Rathod, Sushma Sonawne, Treville Pereira
{"title":"A Comparison of Proliferative Capacity of Reticular and Erosive Variants of Oral Lichen Planus by Argyrophilic Nucleolar Organizer Regions Method.","authors":"Swati Shrikant Gotmare,&nbsp;Anish A Gupta,&nbsp;Mandavi Waghmare,&nbsp;Pratibha Kavle,&nbsp;Asha Rathod,&nbsp;Sushma Sonawne,&nbsp;Treville Pereira","doi":"10.4103/jmau.jmau_104_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_104_20","url":null,"abstract":"<p><strong>Background: </strong>Oral lichen planus (OLP), an immune mediated disorder, has been recognized since 1869 and is presented as any one of the six variants. Reticular and erosive are the most frequently encountered. Its proliferative capacity can give some information regarding its progression. We adopted the argyrophilic nucleolar organizer regions (AgNORs) method because of its simplicity to use and dependable results. We evaluated AgNORs in basal, suprabasal, and squamous cell layers. We also compared these three layers within two variants, reticular, and erosive.</p><p><strong>Materials and methods: </strong>Thirty clinically diagnosed patients of OLP were included in the study. Reticular and erosive variants were included in our study. This was followed by hematoxylin and eosin staining and later by the AgNOR method. The mean number of AgNORs per nucleus was calculated.</p><p><strong>Results: </strong>Thirteen males and 17 females were the gender distribution. Twenty-three (76.67%) had reticular pattern and seven (23.33%) had erosive pattern. The basal cell layer had the highest mean AgNOR compared to suprabasal and squamous layers. Even among, erosive and reticular variants, the former had higher mean AgNOR counts.</p><p><strong>Discussion: </strong>Our results suggest that the inflammatory infiltrate close to the epithelial cells can alter the proliferation index for the pattern of protein synthesis of these cells. Moreover, the high proliferative index in OLP can be related to a specific immunologic response.</p><p><strong>Conclusion: </strong>We conclude that AgNOR can be used as a proliferative marker in earlier lesions to detect the severity.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"12-16"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/b1/JMAU-11-12.PMC10153732.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Howell–Jolly body-like inclusions in a case of puerperal sepsis: A morphological enigma 产褥期败血症病例中的Howell-Jolly体样包涵体:形态学之谜
Journal of Microscopy and Ultrastructure Pub Date : 2023-01-01 DOI: 10.4103/jmau.jmau_28_23
T. Chatterjee, Shilpi More, R. Wadhwa, Erika Rana
{"title":"Howell–Jolly body-like inclusions in a case of puerperal sepsis: A morphological enigma","authors":"T. Chatterjee, Shilpi More, R. Wadhwa, Erika Rana","doi":"10.4103/jmau.jmau_28_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_28_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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