Komal Khare, Prashant Bhusari, Abhishek Soni, Sachin K Malagi, Dennis Abraham, Lynn Johnson
{"title":"A Comparative Evaluation of Efficacy of BioMin F and Propolis Containing Toothpastes on Dentinal Tubule Occlusion with and Without Use of an Adjunct 810 nm Diode Laser: An <i>In vitro</i> Scanning Electron Microscope Study.","authors":"Komal Khare, Prashant Bhusari, Abhishek Soni, Sachin K Malagi, Dennis Abraham, Lynn Johnson","doi":"10.4103/jmau.jmau_133_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_133_20","url":null,"abstract":"<p><strong>Introduction: </strong>Dentinal hypersensitivity (DH) is a common chronic condition that affects a high proportion of the adult population and is one of the main reasons why patients seek dental opinion and treatment. Despite considerable success in reducing DH, unfortunately, most of the present methods can provide only temporary and unpredictable desensitization. Therefore, new treatment methods that are effective for long periods and at the same time do not have the complications are needed. Recently, two new desensitizing agents BioMin F and Propolis have been introduced for the treatment of DH.</p><p><strong>Aim: </strong>The aim of the study was to compare and evaluate the efficacy of BioMin F and Propolis containing toothpastes on dentinal tubule occlusion with and without the use of an adjunct 810 nm Diode Laser.</p><p><strong>Materials and methods: </strong>Forty-five freshly extracted teeth were taken out of which thirty were sectioned into halves and divided into four test groups BioMin F, Propolis, BioMin F + Laser, and Propolis + Laser and control group. All the specimens were treated twice a day for 7 days and then evaluated under scanning electron microscope for partial and complete dentinal tubule occlusion.</p><p><strong>Results: </strong>A significantly higher number of completely occluded tubules were seen in BioMin F + laser group followed by Propolis + laser, Biomin F, and Propolis.</p><p><strong>Conclusion: </strong>Combination approach of desensitizing agent and laser provided a better result than the desensitizing agent alone and when compared individually Biomin F was more effective desensitizer as compared to Propolis.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/27/JMAU-11-41.PMC10153734.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nandhini Gunasekaran, Dineshkumar Thayalan, A. James, A. Vignesh
{"title":"Inappropriate oral habits and mucocele in children","authors":"Nandhini Gunasekaran, Dineshkumar Thayalan, A. James, A. Vignesh","doi":"10.4103/jmau.jmau_18_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_18_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Comparison of Proliferative Capacity of Reticular and Erosive Variants of Oral Lichen Planus by Argyrophilic Nucleolar Organizer Regions Method.","authors":"Swati Shrikant Gotmare, Anish A Gupta, Mandavi Waghmare, Pratibha Kavle, Asha Rathod, Sushma Sonawne, Treville Pereira","doi":"10.4103/jmau.jmau_104_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_104_20","url":null,"abstract":"<p><strong>Background: </strong>Oral lichen planus (OLP), an immune mediated disorder, has been recognized since 1869 and is presented as any one of the six variants. Reticular and erosive are the most frequently encountered. Its proliferative capacity can give some information regarding its progression. We adopted the argyrophilic nucleolar organizer regions (AgNORs) method because of its simplicity to use and dependable results. We evaluated AgNORs in basal, suprabasal, and squamous cell layers. We also compared these three layers within two variants, reticular, and erosive.</p><p><strong>Materials and methods: </strong>Thirty clinically diagnosed patients of OLP were included in the study. Reticular and erosive variants were included in our study. This was followed by hematoxylin and eosin staining and later by the AgNOR method. The mean number of AgNORs per nucleus was calculated.</p><p><strong>Results: </strong>Thirteen males and 17 females were the gender distribution. Twenty-three (76.67%) had reticular pattern and seven (23.33%) had erosive pattern. The basal cell layer had the highest mean AgNOR compared to suprabasal and squamous layers. Even among, erosive and reticular variants, the former had higher mean AgNOR counts.</p><p><strong>Discussion: </strong>Our results suggest that the inflammatory infiltrate close to the epithelial cells can alter the proliferation index for the pattern of protein synthesis of these cells. Moreover, the high proliferative index in OLP can be related to a specific immunologic response.</p><p><strong>Conclusion: </strong>We conclude that AgNOR can be used as a proliferative marker in earlier lesions to detect the severity.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"12-16"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/b1/JMAU-11-12.PMC10153732.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Howell–Jolly body-like inclusions in a case of puerperal sepsis: A morphological enigma","authors":"T. Chatterjee, Shilpi More, R. Wadhwa, Erika Rana","doi":"10.4103/jmau.jmau_28_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_28_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Morphometric analysis of the changes in glomerular podocytes in patients suffereing from proteinuria: An ultrastructural observational study","authors":"SarahHassan Tawfik, Hoda Abutaleb, Tarek Aboushousha, TamerAbdelTawab Hassan, EhabOsama Hafiz","doi":"10.4103/jmau.jmau_39_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_39_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135319156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramiza Munir, Basheer Khan, Ghulam Dastagir, Noor Ul Uza
{"title":"Implication of Scanning Electron Microscopy and Light Microscopy for Morphology of Some Selected Seed Drugs: As a Tool for Authentication.","authors":"Ramiza Munir, Basheer Khan, Ghulam Dastagir, Noor Ul Uza","doi":"10.4103/jmau.jmau_104_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_104_22","url":null,"abstract":"<p><strong>Background: </strong>The goal of our current study is to use Scanning electron microscopy (SEM) and light microscopy (LM) to evaluate the ultra-micromorphological properties of 14 seed drugs to correctly identify and validate them. There was no previous research on SEM-based evaluation of the selected seeds. These included: <i>Solanum nigrum</i> L., <i>Physalis peruviana</i> L., <i>Cestrum diurrnum</i> L., <i>Withania somnifera</i> L. Dunal, <i>Achyranthus aspera</i> L., <i>Celosia argentia</i> L., <i>Chenopodium murale</i> L., <i>Cyperus alternifolius</i> L., <i>Cyperus rotundus</i> L., <i>Schoenoplectus litoralis</i> (Schrad.) Palla, <i>Oxalis corniculata</i> L., <i>Catharanthus roseus</i> L., <i>Canna indica</i> L., and <i>Parthenium hysterophorus</i> L. belonging to 7 families (<i>Solanaceae</i>, <i>Amaranthaceae</i>, <i>Cyperaceae</i>, <i>Oxalidaceae</i>, <i>Apocynaceae</i>, <i>Cannaceae</i>, and <i>Asteraceae</i>).</p><p><strong>Methods: </strong>Quantitative characters (length, width, and weight of seeds) as well as qualitative characters (seed shape, color, texture, and surface level of seeds) were analyzed.</p><p><strong>Results: </strong>Seeds length ranged from 0.6 μm (<i>C. indica</i>) to 2.4 μm (<i>A. aspera</i>) while, the seeds width and weight ranged from 0.6 μm (<i>C. indica</i>) to 1.8 μm (<i>W. somnifera</i>) and 0.03 g (<i>C. indica</i>) to 3.7 g (<i>C. argentia</i>), respectively. The SEM revealed many types of surface texture. Five types of surface levels (raised, regular, smooth, rough, and ill-defined pattern) were observed in seeds. The variation was found to be quite significant for the taxonomic demarcation at generic and specific levels.</p><p><strong>Conclusions: </strong>SEM could be a valuable approach for hidden morphological features of seed drugs, which could aid further exploration, appropriate identification, seed taxonomy, and authenticity. SEM and LM also play an important role in drug discovery and development.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"60-67"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/39/d4/JMAU-11-60.PMC10153735.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9407729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Unusual translocation in a usual leukemia: Follow-up from diagnosis to hematopoietic stem cell transplant","authors":"Tathagata Chatterjee, Shilpi More, Sujata Raychaudhuri, Garima Dhull","doi":"10.4103/jmau.jmau_53_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_53_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Myofibroblasts in Odontogenic Cysts and Tumors: An Immunohistochemical Study.","authors":"Soujanya Pinisetti, Durgaprasad Tadi, Ravikanth Manyam","doi":"10.4103/jmau.jmau_64_21","DOIUrl":"https://doi.org/10.4103/jmau.jmau_64_21","url":null,"abstract":"<p><strong>Objective: </strong>The objective of this study was to assess immunohistochemically the presence of myofibroblasts both qualitatively and quantitatively in odontogenic cysts and tumors and to compare with the control cases of squamous cell carcinoma and to correlate the results with biologic behavior of these lesions.</p><p><strong>Materials and methodology: </strong>Formalin-fixed, paraffin-embedded blocks of odontogenic cysts and tumors were retrieved from institutional archives. The sample size is 40; these include ten cases of odontogenic keratocyst (OKC) (<i>n</i> = 10), five cases of dentigerous cyst (<i>n</i> = 5), ten cases of solid ameloblastoma (<i>n</i> = 10), and five cases of unicystic ameloblastoma (<i>n</i> = 5). Ten cases of squamous cell carcinoma (<i>n</i> = 10) served as control. Sections were taken and stained immunohistochemically using alpha-smooth muscle actin for evaluation of myofibroblasts. The number of positive stromal cells was evaluated both for quantitative and qualitative analyses.</p><p><strong>Results: </strong>The present study showed that the mean number of myofibroblasts among the odontogenic cysts and tumors was higher in locally aggressive lesions such as OKC (23.79 ± 19.95), solid ameloblastoma (26.38 ± 17.00), and unicystic ameloblastoma (20.74 ± 14.86) which were comparable to squamous cell carcinoma (21.49 ± 9.76) when compared to benign lesions like dentigerous cyst which showed the least number of myofibroblasts (13.1 ± 7.71). Qualitatively, the staining intensity of myofibroblasts showed a significant variation within the same lesion and among different lesions. There was a distinct difference in the morphology, pattern of arrangement, and distribution of myofibroblasts among the studied lesions.</p><p><strong>Conclusion: </strong>We conclude that the increase in the number of myofibroblasts could be one of the contributory factors for the locally aggressive behavior of benign lesions such as ameloblastomas and OKCs. Further studies are suggested to understand the mechanism by which these important cellular elements exert their effects on stromal and epithelial tissue compartments.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/78/2b/JMAU-11-68.PMC10153737.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9415560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Vaishali Shirale, SudhirKumar Jain, RituKumari Saxena, AjayKumar Saxena, S. Rustagi
{"title":"Evaluation of accuracy of ultrasound guided needle aspiration cytology in the assessment of axilla in breast carcinoma and its correlation with histopathological findings","authors":"Vaishali Shirale, SudhirKumar Jain, RituKumari Saxena, AjayKumar Saxena, S. Rustagi","doi":"10.4103/jmau.jmau_102_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_102_22","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The usefulness of gray image fractal analysis – An adjunct tool for the diagnosis of breast lesions at cytology","authors":"K. Amita, Kalappa Prashantha, Rajani Gowda","doi":"10.4103/jmau.jmau_110_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_110_22","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70799996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}