Journal of Microscopy and Ultrastructure最新文献

{"title":"Development of the Hair Cells of the Human Cochlea: A Scanning Electron Microscopic Study.","authors":"Sabita Mishra,&nbsp;Tarasankar Roy,&nbsp;Shubhi Saini","doi":"10.4103/JMAU.JMAU_107_20","DOIUrl":"https://doi.org/10.4103/JMAU.JMAU_107_20","url":null,"abstract":"<p><strong>Introduction: </strong>In the mammalian auditory system, the cochlea is the first to attain structural and functional maturity. Although ultrastructural details of the developing cochlea of lower animals have been elucidated in the last few decades, comprehensive studies on human cochlea are lacking.</p><p><strong>Materials and methods: </strong>In the present investigation we studied the development and maturation of the hair cells of ten human fetal cochlea from gestational weeks (GW) 12 to 37 by scanning electron microscopy.</p><p><strong>Result: </strong>We observed undifferentiated hair cells possessing numerous surface projections and long kinocilium during GW 14. At GW16, the primitive hair cells were arranged in one inner and four outer rows and had globular apices indicating the initiation of stereocilia formation. By GW 22, the globular apices were replaced by linear stereocilia and occasional kinocillia. Mature hair cells with sterocilia were observed in the basal turn at 30th week of gestation. At GW 37, the stereocilia were arranged in a typical \"V\" shaped pattern at the middle and apical coil, while the stereocilia of the basal turn were shorter in length resembling the adult cochlea. The inner hair cells were long and slender while outer hair cells were pear shaped, kinocilium were absent and the tunnel of Corti were well formed.</p><p><strong>Conclusion: </strong>It is concluded that in human, the morphological maturation of the hair cells starts in the basal turn around GW 22 and continues till 37th week in the apical turn indicating that early maturation of the cochlea may have a role on development of the higher auditory pathway connections.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/bf/1e/JMAU-11-17.PMC10153736.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9415553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioglitazone Induces Dysplastic Urothelial Changes in Urinary Bladder of Experimental Diabetes.","authors":"Ahmed A M Abdel-Hamid,&nbsp;Alaa El-Din L Firgany","doi":"10.4103/jmau.jmau_34_21","DOIUrl":"https://doi.org/10.4103/jmau.jmau_34_21","url":null,"abstract":"<p><strong>Objectives: </strong>Pioglitazone (PIO) is a widely prescribed oral antidiabetic drug that has concerns regarding a potential risk of developing carcinoma of the urinary bladder. The objective of the current study was to assess this potential risk.</p><p><strong>Materials and methods: </strong>The potential risk of PIO-induced urinary bladder carcinoma was assessed in the current study by examining urinary bladder of rats for urothelial cytokeratin (CK) expression and proliferative activity by Ki67 immunostaining.</p><p><strong>Results: </strong>Histological examination revealed dysplastic urothelial changes in PIO <i>per se</i> and diabetes mellitus + PIO (diabetic rats receiving PIO). In addition, a significantly (<i>P</i> < 0.05) decreased CK7 and CK8 expression together with a significantly increased CK20 as well as Ki67 expression was detected in the urothelial cells of groups administrated PIO, contrary to those which did not.</p><p><strong>Conclusion: </strong>The manifestations of urothelial dysplasia evidenced by histological examination as well as by the aberrant expression in CK and Ki67 after PIO administration add supporting evidence at cellular and experimental level to the previous clinical suspicions.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"34-40"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/62/c5/JMAU-11-34.PMC10153741.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9415554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal changes in the localization of cytokeratin AE1, alpha-smooth muscle actin, and S100 proteins in the testis and epididymis of the African Fruit Bat (Epomops franqueti","authors":"O. Ekeolu, S. Olukole, B. Oke","doi":"10.4103/jmau.jmau_121_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_121_22","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Evaluation of Efficacy of BioMin F and Propolis Containing Toothpastes on Dentinal Tubule Occlusion with and Without Use of an Adjunct 810 nm Diode Laser: An <i>In vitro</i> Scanning Electron Microscope Study.","authors":"Komal Khare,&nbsp;Prashant Bhusari,&nbsp;Abhishek Soni,&nbsp;Sachin K Malagi,&nbsp;Dennis Abraham,&nbsp;Lynn Johnson","doi":"10.4103/jmau.jmau_133_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_133_20","url":null,"abstract":"<p><strong>Introduction: </strong>Dentinal hypersensitivity (DH) is a common chronic condition that affects a high proportion of the adult population and is one of the main reasons why patients seek dental opinion and treatment. Despite considerable success in reducing DH, unfortunately, most of the present methods can provide only temporary and unpredictable desensitization. Therefore, new treatment methods that are effective for long periods and at the same time do not have the complications are needed. Recently, two new desensitizing agents BioMin F and Propolis have been introduced for the treatment of DH.</p><p><strong>Aim: </strong>The aim of the study was to compare and evaluate the efficacy of BioMin F and Propolis containing toothpastes on dentinal tubule occlusion with and without the use of an adjunct 810 nm Diode Laser.</p><p><strong>Materials and methods: </strong>Forty-five freshly extracted teeth were taken out of which thirty were sectioned into halves and divided into four test groups BioMin F, Propolis, BioMin F + Laser, and Propolis + Laser and control group. All the specimens were treated twice a day for 7 days and then evaluated under scanning electron microscope for partial and complete dentinal tubule occlusion.</p><p><strong>Results: </strong>A significantly higher number of completely occluded tubules were seen in BioMin F + laser group followed by Propolis + laser, Biomin F, and Propolis.</p><p><strong>Conclusion: </strong>Combination approach of desensitizing agent and laser provided a better result than the desensitizing agent alone and when compared individually Biomin F was more effective desensitizer as compared to Propolis.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"41-46"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cb/27/JMAU-11-41.PMC10153734.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9784178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inappropriate oral habits and mucocele in children","authors":"Nandhini Gunasekaran, Dineshkumar Thayalan, A. James, A. Vignesh","doi":"10.4103/jmau.jmau_18_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_18_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparison of Proliferative Capacity of Reticular and Erosive Variants of Oral Lichen Planus by Argyrophilic Nucleolar Organizer Regions Method.","authors":"Swati Shrikant Gotmare,&nbsp;Anish A Gupta,&nbsp;Mandavi Waghmare,&nbsp;Pratibha Kavle,&nbsp;Asha Rathod,&nbsp;Sushma Sonawne,&nbsp;Treville Pereira","doi":"10.4103/jmau.jmau_104_20","DOIUrl":"https://doi.org/10.4103/jmau.jmau_104_20","url":null,"abstract":"<p><strong>Background: </strong>Oral lichen planus (OLP), an immune mediated disorder, has been recognized since 1869 and is presented as any one of the six variants. Reticular and erosive are the most frequently encountered. Its proliferative capacity can give some information regarding its progression. We adopted the argyrophilic nucleolar organizer regions (AgNORs) method because of its simplicity to use and dependable results. We evaluated AgNORs in basal, suprabasal, and squamous cell layers. We also compared these three layers within two variants, reticular, and erosive.</p><p><strong>Materials and methods: </strong>Thirty clinically diagnosed patients of OLP were included in the study. Reticular and erosive variants were included in our study. This was followed by hematoxylin and eosin staining and later by the AgNOR method. The mean number of AgNORs per nucleus was calculated.</p><p><strong>Results: </strong>Thirteen males and 17 females were the gender distribution. Twenty-three (76.67%) had reticular pattern and seven (23.33%) had erosive pattern. The basal cell layer had the highest mean AgNOR compared to suprabasal and squamous layers. Even among, erosive and reticular variants, the former had higher mean AgNOR counts.</p><p><strong>Discussion: </strong>Our results suggest that the inflammatory infiltrate close to the epithelial cells can alter the proliferation index for the pattern of protein synthesis of these cells. Moreover, the high proliferative index in OLP can be related to a specific immunologic response.</p><p><strong>Conclusion: </strong>We conclude that AgNOR can be used as a proliferative marker in earlier lesions to detect the severity.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"12-16"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/8d/b1/JMAU-11-12.PMC10153732.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9416899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Howell–Jolly body-like inclusions in a case of puerperal sepsis: A morphological enigma","authors":"T. Chatterjee, Shilpi More, R. Wadhwa, Erika Rana","doi":"10.4103/jmau.jmau_28_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_28_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70800635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric analysis of the changes in glomerular podocytes in patients suffereing from proteinuria: An ultrastructural observational study","authors":"SarahHassan Tawfik, Hoda Abutaleb, Tarek Aboushousha, TamerAbdelTawab Hassan, EhabOsama Hafiz","doi":"10.4103/jmau.jmau_39_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_39_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135319156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Implication of Scanning Electron Microscopy and Light Microscopy for Morphology of Some Selected Seed Drugs: As a Tool for Authentication.","authors":"Ramiza Munir,&nbsp;Basheer Khan,&nbsp;Ghulam Dastagir,&nbsp;Noor Ul Uza","doi":"10.4103/jmau.jmau_104_22","DOIUrl":"https://doi.org/10.4103/jmau.jmau_104_22","url":null,"abstract":"<p><strong>Background: </strong>The goal of our current study is to use Scanning electron microscopy (SEM) and light microscopy (LM) to evaluate the ultra-micromorphological properties of 14 seed drugs to correctly identify and validate them. There was no previous research on SEM-based evaluation of the selected seeds. These included: <i>Solanum nigrum</i> L., <i>Physalis peruviana</i> L., <i>Cestrum diurrnum</i> L., <i>Withania somnifera</i> L. Dunal, <i>Achyranthus aspera</i> L., <i>Celosia argentia</i> L., <i>Chenopodium murale</i> L., <i>Cyperus alternifolius</i> L., <i>Cyperus rotundus</i> L., <i>Schoenoplectus litoralis</i> (Schrad.) Palla, <i>Oxalis corniculata</i> L., <i>Catharanthus roseus</i> L., <i>Canna indica</i> L., and <i>Parthenium hysterophorus</i> L. belonging to 7 families (<i>Solanaceae</i>, <i>Amaranthaceae</i>, <i>Cyperaceae</i>, <i>Oxalidaceae</i>, <i>Apocynaceae</i>, <i>Cannaceae</i>, and <i>Asteraceae</i>).</p><p><strong>Methods: </strong>Quantitative characters (length, width, and weight of seeds) as well as qualitative characters (seed shape, color, texture, and surface level of seeds) were analyzed.</p><p><strong>Results: </strong>Seeds length ranged from 0.6 μm (<i>C. indica</i>) to 2.4 μm (<i>A. aspera</i>) while, the seeds width and weight ranged from 0.6 μm (<i>C. indica</i>) to 1.8 μm (<i>W. somnifera</i>) and 0.03 g (<i>C. indica</i>) to 3.7 g (<i>C. argentia</i>), respectively. The SEM revealed many types of surface texture. Five types of surface levels (raised, regular, smooth, rough, and ill-defined pattern) were observed in seeds. The variation was found to be quite significant for the taxonomic demarcation at generic and specific levels.</p><p><strong>Conclusions: </strong>SEM could be a valuable approach for hidden morphological features of seed drugs, which could aid further exploration, appropriate identification, seed taxonomy, and authenticity. SEM and LM also play an important role in drug discovery and development.</p>","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"11 1","pages":"60-67"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/39/d4/JMAU-11-60.PMC10153735.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9407729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusual translocation in a usual leukemia: Follow-up from diagnosis to hematopoietic stem cell transplant","authors":"Tathagata Chatterjee, Shilpi More, Sujata Raychaudhuri, Garima Dhull","doi":"10.4103/jmau.jmau_53_23","DOIUrl":"https://doi.org/10.4103/jmau.jmau_53_23","url":null,"abstract":"","PeriodicalId":16340,"journal":{"name":"Journal of Microscopy and Ultrastructure","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70801309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}