Journal of food protection最新文献

{"title":"Development and Validation of a Novel Classification Framework for Voluntary Food Safety Adoption Among Small-Scale Produce Growers: A Pilot Study.","authors":"Jesica Temple, Jessica M Blythe, Tim P Boltz, Arron J Giorgi, Christopher M Ashwell, Cangliang Shen","doi":"10.1016/j.jfp.2025.100630","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100630","url":null,"abstract":"<p><p>This study utilizes a novel classification framework to address voluntary food safety practices among small-scale produce growers who sell primarily at West Virginia farmers' markets. Limited data exist on how these exempt growers voluntarily adopt post-harvest food safety practices. This pilot study assessed post-harvest food safety behaviors and introduced a behavioral classification framework-Willing, Ready, and Able (WRA)-to stratify engagement among FSMA-exempt small-scale produce growers. A cross-sectional survey was administered in 2025 to 40 growers at the West Virginia Small Farms Conference. FSMA exemption was confirmed by self-report or eligibility criteria; five FSMA-covered respondents were excluded, yielding a final sample of 35. Growers were categorized as FSMA-Willing (no training, confident in current practices), FSMA-Ready (training plus adoption of one to three Good Agricultural Practices(GAPs)aligned practices), or FSMA-Able (training plus adoption of four or more GAPs-aligned practices). Among FSMA-exempt respondents (n = 35), 62.8% reported training, 53.1% sanitized surfaces, and 52.5% used refrigeration. WRA classification revealed that 34.3% were Willing, 34.3% Ready, and 28.5% Able. The WRA framework introduces a novel behavioral classification system to differentiate FSMA-exempt growers by readiness, training history, and current safety practices. These findings suggest many exempt growers voluntarily implement risk-reducing behaviors, and WRA can guide targeted outreach to improve food safety in decentralized systems.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100630"},"PeriodicalIF":2.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Farm-to-consumer quantitative microbial risk assessment model for Listeria monocytogenes on fresh-cut cantaloupe.","authors":"Sarah Ingersoll Murphy, Ece Bulut, Laura K Strawn, Michelle D Danyluk, Martin Wiedmann, Renata Ivanek","doi":"10.1016/j.jfp.2025.100626","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100626","url":null,"abstract":"<p><p>Cantaloupe contamination with the foodborne pathogen Listeria monocytogenes (LM) may occur along the supply chain. We developed a quantitative microbial risk assessment (QMRA) model for LM on cantaloupe along the fresh-cut supply chain and evaluated potential risk reduction strategies. The developed model starts at harvest and includes conditions during transportation from field to intermediate facility (packinghouse or cooling facility), handling at the intermediate facility, transportation to the fresh-cut facility, storage pre-processing at the fresh-cut facility, processing and handling at the fresh-cut facility, as well as conditions during distribution, retail, transportation to home, and home storage. The model was simulated to (i) provide an estimate of LM concentration in a single serving (134 g) and (ii) estimate annual illnesses and deaths in the United States attributed to LM contaminated fresh-cut cantaloupe. The baseline model predicted the median risk of listeriosis per serving in general and susceptible populations was 1.4 x 10^-12 and 6.4 x 10^-11, respectively. The median (5^th, 95^th percentiles) predicted number of illnesses and deaths annually attributed to fresh-cut cantaloupe were 0 (0, 1070) and 0 (0, 264), respectively. Time and temperature conditions post-packaging, and the initial number of LM at harvest had the greatest impacts on LM per contaminated serving and the number of annual illnesses; the initial LM levels at harvest and cross-contamination parameters at the fresh-cut facility had the greatest impacts on prevalence of contaminated servings. Assessment of interventions demonstrated that reducing temperature and/or time conditions post-packaging can be an effective risk reduction strategy. Overall, the developed tool estimates the risk associated with the consumption of LM contaminated fresh-cut cantaloupe and facilitates the identification and assessment of potential risk reduction strategies across the supply chain.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100626"},"PeriodicalIF":2.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly Pathogenic Avian Influenza (HPAI) H5N1 in Raw Pet Foods and Milk: A Growing Threat to both Companion Animals and Human Health, and Potential Raw Pet Food Industry Liability.","authors":"Janak Dhakal, Sushant Bhat, Joe James, Richard Y Otwey, Sandesh Chapagain, Parminder Singh","doi":"10.1016/j.jfp.2025.100628","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100628","url":null,"abstract":"<p><p>The increasing popularity of raw meat-based diets (RMBDs) and raw milk feeding in companion animals presents a growing concern for zoonotic disease transmission. Recent evidence has demonstrated that these products can serve as vehicles for highly pathogenic avian influenza (HPAI) H5N1, an emergent viral threat with a host range from birds, dairy cattle, and pets to humans. Since the emergence of clade 2.3.4.4b in 2020, HPAI H5N1 has caused widespread outbreaks in poultry, wild birds, and mammals, including dairy cattle and cats. Transmission to pets has been linked to ingestion of contaminated raw pet food and unpasteurized milk. Notably, multiple outbreaks in cats across Europe, Asia, and North America have been associated with raw pet food products, while recent U.S. cases confirm direct viral transmission from infected pet food, raw milk, and colostrum. Experimental studies have also supported the plausibility of gastrointestinal and respiratory routes of infection in cats and dogs, with felines appearing particularly susceptible, often exhibiting severe clinical disease and high mortality. A number of documented recalls of H5N1-contaminated raw pet food and raw milk in the US underscore the persistence of infectious viruses in cold-stored food products and highlight the risks of feeding raw diets. Although pet-to-human transmission of the HPAI H5N1 virus has not been reported yet, cat-to-human transmission of the H7N2 influenza virus has been reported in the USA. This review presents current evidence on H5N1 in RMBDs and raw milk, its epidemiology in companion animals, outbreaks, and the health implications among pets and humans. By raising awareness among pet owners, industry stakeholders, and veterinarians, this paper highlights the immediate need for stringent surveillance and improved biosecurity in raw food supply chains to minimize viral transmission risks thereby safeguarding pet health and curb the potential spillover to humans.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100628"},"PeriodicalIF":2.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Salmonella recovery from commercial poultry environmental samples with selective pre-enrichment.","authors":"Amy T Siceloff, Nikki W Shariat","doi":"10.1016/j.jfp.2025.100627","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100627","url":null,"abstract":"<p><p>The current, culture-based methods for detecting Salmonella are time and resource intensive, as it can take between three to five days with pre-enrichment and selective enrichment steps. Previous work by our group has shortened this process by combining novobiocin and selective ingredients from Rappaport-Vassiliadis (RV) (malachite green; 0.1 g/L) and tetrathionate (TT) (bile salts; 1 g/L) to BPW in parallel, creating an all-encompassing selective pre-enrichment step. In this study, we sought to validate the use of selective pre-enrichment on commercial poultry live production samples, as the increased presence of background bacteria may limit Salmonella recovery. Two pairs of boot sock samples were collected from 35 houses, representing 17 different commercial broiler or breeder farms (n = 70 samples). The samples were cultured under selective pre-enrichment conditions in parallel with standard non-selective pre-enrichment (BPW) followed by selective enrichment (RV, TT). Additionally, molecular enumeration was performed to quantify the amount of Salmonella present in each sample. Overall, Salmonella was found in 74% (52/70) of samples collected, and selective pre-enrichment and selective enrichment conditions each recovered Salmonella in 14/17 farms. The average quantity per sample was greater in those recovered with selective pre-enrichment (5.2 log₁₀ CFU/sample) than those that were not (3.0 log₁₀ CFU/sample; p = 0.01, Welch two sample t-test). CRISPR-SeroSeq was used to determine the relative frequency of Salmonella serovars in each sample and culture condition. The proportion of multiserovar populations observed in the selective pre-enrichment conditions (53%, 29/55) was not significantly different from those in selective enrichment conditions (56%, 39/70; p = 0.1, McNemar's chi-squared test). These findings suggest that increasing the selectivity of the Salmonella pre-enrichment step could eliminate the need for a subsequent selective enrichment, thus reducing the time to Salmonella isolation by 24 hours.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100627"},"PeriodicalIF":2.8,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145186060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Veterinary Laboratory Investigation and Response Network: 15 Years of Promoting Human and Animal Health by Collaborating with the Veterinary Diagnostic Laboratory Community.","authors":"Sarah M Nemser, Olgica Ceric, Jake Guag, Sarah Pauley, Angelica Jones, Kathleen Proia, Megan R Miller, Andriy Tkachenko, David Rotstein, April Hodges, Renate Reimschuessel, Gregory H Tyson","doi":"10.1016/j.jfp.2025.100625","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100625","url":null,"abstract":"<p><p>The Veterinary Laboratory Investigation and Response Network (Vet-LIRN), a collaborative network established in 2010, is a partnership between the Food and Drug Administration's Center for Veterinary Medicine (FDA CVM) and 48 veterinary diagnostic laboratories (VDLs) across North America. Vet-LIRN actively supports the CVM mission of protecting human and animal health by leveraging its network of VDLs. Initially focused on issues in animal foods, including by testing animal diagnostic samples, Vet-LIRN now addresses a broad range of CVM's priorities. These include responding to animal foodborne illness outbreaks, developing new methods to detect potential microbial and chemical contaminants in animal foods, tracking antimicrobial resistance (AMR), promoting antimicrobial stewardship in veterinary medicine, and preparing for emerging One Health threats such as COVID-19 and Highly Pathogenic Avian Influenza (HPAI). Over the past 15 years, Vet-LIRN has played a pivotal role in many high-profile and important public health success stories, such as responding to multidrug-resistant Campylobacter outbreaks in puppies, aflatoxin contamination in pet food, Salmonella in pig ear treats, and botulinum toxin in alfalfa cubes. Additionally, Vet-LIRN's AMR monitoring program collects data to understand AMR trends and assist in the response to foodborne and zoonotic outbreaks. Through collaboration with other key stakeholders such as CVM regulatory colleagues and external partners at the United States Department of Agriculture (USDA) and the Centers for Disease Control and Prevention (CDC), Vet-LIRN ensures rapid responses to critical issues. Looking ahead, Vet-LIRN remains dedicated to continuous improvements, reinforcing its commitment to the sustained protection of human and animal health.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100625"},"PeriodicalIF":2.8,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145181951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Contamination on Kitchen Surfaces in University Student Housing: Insights from Swab Analysis and Behavioral Surveys","authors":"Gustavo Guimarães Fernandes Viana ,&nbsp;Ana Julia Pereira Mello ,&nbsp;Beatriz da Apresentação ,&nbsp;Danilo dos Santos Gonçalves ,&nbsp;Eduarda Yamauti Gerolamo ,&nbsp;Giulya Monteiro de Castro ,&nbsp;Guilherme Cardoso da Silva ,&nbsp;Henrique de Rezende Corá ,&nbsp;Izabella Regina da Silva Marcelino ,&nbsp;Kaedra Piva Busch ,&nbsp;Luana Kleinubing Aguiar ,&nbsp;Luiza Mattos Mendonça ,&nbsp;Max Sândalo Ferreira da Silva ,&nbsp;Mayara de Sousa Canute ,&nbsp;Miguel Sionti de Medeiros Paulino ,&nbsp;Pedro Barasnevicius da Silva ,&nbsp;Pedro Caldeira de Araújo ,&nbsp;Talita Duran Semedo ,&nbsp;Victoria Ribeiro Silvestre ,&nbsp;Vinicius Guilherme de Araújo ,&nbsp;Juliano Gonçalves Pereira","doi":"10.1016/j.jfp.2025.100624","DOIUrl":"10.1016/j.jfp.2025.100624","url":null,"abstract":"<div><div>Shared kitchens are potential hotspots for microbial contamination due to frequent use and poor hygiene. In student residences, these risks are heightened by diverse hygiene behaviors and limited oversight. This study aimed to evaluate microbial contamination and hygiene-related behaviors in university student residences. Thirty student households in Botucatu, Brazil, were investigated through microbiological sampling of kitchen surfaces (refrigerator, sink, dish towels, and sponge). Samples were analyzed to assess the counts of mesophilic aerobic bacteria and <em>Enterobacteriaceae</em>, and the detection of <em>Salmonella</em> spp. and <em>L. monocytogenes</em>. In addition, a structured questionnaire was applied to gather data on hygiene routines and food handling practices. Feedback sessions were held to communicate the results to each household. No samples tested positive for <em>Salmonella</em> spp. or <em>L. monocytogenes</em>. Sponges exhibited the highest levels of microbial contamination, with median counts of 8.63 log CFU/cm² for mesophiles and 5.72 log CFU/cm² for <em>Enterobacteriaceae</em>, followed by dish towels (4.11 and 3.60 log CFU/cm²), sinks (1.27 and 0.24 log CFU/cm²), and refrigerators (−0.25 and −0.18 log CFU/cm²). Some associations between hygiene behaviors and microbial load were observed, including differences linked to dishwashing habits and the number of residents using the refrigerator. The descriptive trends suggest that certain behaviors, such as the frequency of dish towel sanitization and refrigerator cleaning, may influence microbial risks. These findings emphasize the importance of consistent hygiene practices in shared kitchen environments and support the development of educational and preventive strategies aimed at improving food safety among young adults living in communal housing, such as promoting proper dish towel and sponge replacement, clear cleaning responsibilities, and separation of utensils for raw and cooked foods.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 11","pages":"Article 100624"},"PeriodicalIF":2.8,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145113421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Food Safety Standards Requirement Setting and GAP Audit Program Acceptance Decision-making by U.S. Buyers","authors":"Jelili Adegboyega Adebiyi ,&nbsp;Leslie D. Bourquin","doi":"10.1016/j.jfp.2025.100620","DOIUrl":"10.1016/j.jfp.2025.100620","url":null,"abstract":"<div><div>The US Department of Agriculture Agricultural Marketing Service (USDA AMS) developed suites of voluntary fee-for-service Good Agricultural Practices (GAPs) audit verification programs, which, for reasons not well understood, are underwhelmingly accepted by produce buyers. To explore this low acceptance, this study conceptualized that buyers set food quality and safety requirements that their suppliers must meet to access their markets. These requirements likely influence which audit programs buyers deem acceptable. To date, no study has examined how buyers set these requirements or how such decisions shape their acceptance of GAP audit schemes. Therefore, this study surveyed and interviewed buyers to understand their food safety requirements for various types of produce, suppliers, and operations, as well as how these factors influence their selection of audit programs. The resulting information was used to understand the implications of buyers’ food safety requirements for the low acceptance of USDA GAP audit schemes. The findings revealed that buyers’ decisions regarding food safety requirements and audit acceptance are complex, shaped by numerous contextual factors, including regulatory demands, supplier and their operation types, operation scale and location, brand types, retailer reputation, produce risk profiles, and the type, scope, and stringency of audit schemes. The primary factors were the buyers’ customers’ requirements. Beyond fostering food safety, the requirements were set to strengthen buyers’ reputation and competitiveness, enhance quality assurance, and help them capture a share of the produce market. The study concludes by discussing the implications of these findings for expanding acceptance of USDA GAP audit schemes.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 11","pages":"Article 100620"},"PeriodicalIF":2.8,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145058546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitigating Gluten Cross-Contact in Shared Frying Oil through Passive and Active Filtration","authors":"Xingyi Jiang ,&nbsp;Robert Beverly ,&nbsp;Aravind Kumar Bingi ,&nbsp;Qinchun Rao ,&nbsp;Jeremiah Kidd ,&nbsp;Karen Swajian ,&nbsp;Jane Cluster ,&nbsp;Lauren Jackson","doi":"10.1016/j.jfp.2025.100618","DOIUrl":"10.1016/j.jfp.2025.100618","url":null,"abstract":"<div><div>Food manufacturers commonly use filtration with or without filter aids to extend the shelf life of frying oil by removing undesirable substances that form during frying. However, limited research has evaluated the effectiveness of these methods in removing proteins that transfer into frying oil from fried foods. The objective of this study was to assess the efficiency of different filtration methods for removing gluten residues from frying oil. For passive filtration, oil spiked with isolated gluten at a level of 1,000 µg gluten/g oil and oil used for frying 10 batches of breaded shrimp (75 g/batch) were filtered under gravity through metal sieves (pore sizes: 25 µm-2 mm) and cellulose filter papers (pore sizes: 11–25 µm). For active filtration condition selection, gluten-spiked oil (5,000 µg gluten/g oil) was heated (180 °C for 3 min), cooled to 105 °C, and then treated with filter aids – diatomaceous earth (Celite 535) and perlite (Harborlite 900) – at concentrations ranging from 0.05 to 1%, with mixing times of 5–30 min at 1,000 rpm. Subsequently, the oil was filtered through metal sieves (pore sizes: 0.15, 0.6, and 2 mm). Response surface methodology was employed to identify optimal conditions based on their ability to minimize residual gluten. The optimal condition (0.5% filter aid concentration, 30 min mixing time, and 0.15 mm pore size) was then applied to a total of six filter aids. Under these conditions, passive filtration resulted in over 80% reduction in gluten content of oil while the filtration efficiency of active filtration methods was greater than 99.7%. Removal efficiency varied among the six filter aids, with magnesium silicate and diatomaceous earth-based filter aids demonstrating the best performance. Overall, this study highlighted both passive and active filtration as a promising strategy to significantly minimize gluten cross-contact risks associated with reused frying oil, providing practical guidance for gluten management during frying operations.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 11","pages":"Article 100618"},"PeriodicalIF":2.8,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145053990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal Inactivation of Cryptosporidium parvum Oocysts in Soil and Manure Microenvironments Using the LSTM-based Environmental Model","authors":"Manish Thapaliya ,&nbsp;Magesh Rajasekaran ,&nbsp;Adriano F. Vatta ,&nbsp;Jack N. Losso ,&nbsp;Achyut Adhikari","doi":"10.1016/j.jfp.2025.100617","DOIUrl":"10.1016/j.jfp.2025.100617","url":null,"abstract":"<div><div><em>Cryptosporidium parvum</em> is a resilient zoonotic parasite with a high potential for environmental persistence, particularly in agricultural soils and manures. This study introduces a novel application of Long Short-Term Memory (LSTM) deep learning, an artificial intelligence model, to simulate realistic seasonal diurnal temperature and relative humidity cycles based on historical climate data from Baton Rouge, Louisiana, USA. The predicted conditions for summer (21–42 °C; 34–96% RH) and winter (1–18 °C; 50–90% RH) were applied in a controlled growth chamber to study oocyst inactivation over 30 days in soil and manure microenvironments. Inactivation followed first-order kinetics, with significantly higher decay rates observed in manure under summer conditions (<em>k</em> = −0.01379 day⁻¹) compared to winter (<em>k</em> = −0.00405 day⁻¹). Soil showed consistently slower inactivation rates than manure across both seasons. ANOVA and posthoc analyses confirmed the significance of temperature, substrate type, and their interaction on oocyst decay (<em>p</em> &lt; 0.05). These findings highlight the critical influence of temperature and substrate properties on oocyst persistence and underscore the potential of LSTM-based climate modeling to improve environmental pathogen risk assessments under dynamic seasonal conditions.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 11","pages":"Article 100617"},"PeriodicalIF":2.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formation and Control of Listeria monocytogenes Growth and Accumulation on Food Processing Surfaces in the Fresh Produce Industry.","authors":"Aysu Deniz, Blanca Ruiz-Llacsahuanga, Faith Critzer, Dan L Boyle, Josefina Lacasa, Umut Yucel, Valentina Trinetta","doi":"10.1016/j.jfp.2025.100619","DOIUrl":"10.1016/j.jfp.2025.100619","url":null,"abstract":"<p><p>The ability of L. monocytogenes, a common foodborne pathogen, to form biofilms significantly contributes to its persistence and contamination risks. Understanding L. monocytogenes aggregation is crucial to develop effective control strategies. This study assessed the growth and accumulation of L. monocytogenes on common food processing surfaces in the produce industry and evaluated the efficacy of commercially available sanitizers alone or simultaneously with UV-C light for 30 s, 1 min, or 5 min. L. monocytogenes was allowed to aggregate on stainless steel, nylon, high-density polyethylene (HDPE), polyvinyl chloride (PVC), and Teflon for 24 h (initial biofilm aggregate) and 96 h (mature biofilms). Treatments included 120 ppm peracetic acid (PAA), silver dihydrogen citrate (SDC), 4% lactic acid, and UV-C light (254 nm). After sanitizer application, cells were dislodged and enumerated by serial dilution and plating. Confocal images were obtained before and after treatments to evaluate biofilm architecture. All liquid sanitizers significantly reduced L. monocytogenes independently of the surface type (P < 0.05). UV-C treatment application time had no significant effect on biofilm reduction (P > 0.05), though its efficacy on 24 h aggregates varied significantly depending on the surface material. Peracetic acid and lactic acid were the most effective treatments across surfaces, while UV-C alone was the least effective. Combined sanitizer and UV-C light application for up to 5 min did not necessarily improve treatment efficacy compared to sanitizers alone. Confocal images displayed surface cleanability and alterations in microbial architecture before and after treatments. Mature biofilms exhibited extensive surface coverage on all surfaces, which was visibly reduced following sanitizer application. This study provides insightful information to the produce industry for using sanitizing approaches on various surfaces based on cleanability, topographical, and architectural characteristics.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100619"},"PeriodicalIF":2.8,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}