Journal of food protection最新文献

{"title":"Systematic review and meta-analysis of toxic element accumulation in Iranian wheat cultivation.","authors":"Ali Atamaleki, Yadolah Fakhri, Sepideh Sadeghi, Fatemeh Amereh, Masoumeh Rahmatinia, Ali Paseban, Mohammad Sadeghi","doi":"10.1016/j.jfp.2025.100558","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100558","url":null,"abstract":"<p><p>The contamination of wheat with potentially toxic elements (PTEs) poses a significant public health concern, particularly in developing countries where industrial activities and unsustainable agricultural practices contribute to heavy metal accumulation. This systematic review and meta-analysis evaluated concentrations of inorganic arsenic (iAs), cadmium (Cd), mercury (Hg), nickel (Ni), and lead (Pb) in wheat cultivated across Iran. Forty-five papers with 383 data-reports from 1983 to 2023 were included in our study. The highest concentrations were found in Isfahan (iAs: 916.743 μg/kg), Chaharmahal and Bakhtiari (Cd: 884.113 μg/kg), Hamedan (Hg: 84.452 μg/kg), Tehran (Ni: 24000 μg/kg) and Kurdistan (Pb: 2115.454 μg/kg), surpassing international safety standards. Using the Target Hazard Quotient (THQ) indices, the study revealed significant non-carcinogenic and carcinogenic risks, especially for children. The findings emphasize the need for urgent measures to reduce PTEs contamination, including rigorous monitoring of soil, water, and crop quality, sustainable farming practices, and improved wastewater management. Public awareness campaigns and targeted interventions are also critical in mitigating health risks associated with heavy metal exposure. These strategies aim to safeguard public health and reduce the burden of PTE-related health risks, particularly among vulnerable populations.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100558"},"PeriodicalIF":2.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ten-year survey of bacterial pathogens in fresh, unprocessed fruits and vegetables produced, imported or traded in the Netherlands.","authors":"Thomas De Bock, Liesbeth Jacxsens, Frank Devlieghere, Mieke Uyttendaele","doi":"10.1016/j.jfp.2025.100560","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100560","url":null,"abstract":"<p><p>Foodborne outbreaks have raised concern about the microbial safety of fresh produce. Comprehensive data on key bacterial pathogens in these products remains scarce. This study explores the prevalence of foodborne pathogens in unprocessed fruits and vegetables, focusing on the variability according to a temporal scale, the geographical origin, and the product type. A total of 12 808 fresh produce samples were collected over a period of ten years (2013-2022), and analyzed for Salmonella spp., Shiga-toxin producing E. coli (STEC), L. monocytogenes, presumptive B. cereus, and coagulase-positive staphylococci. Overall, L. monocytogenes was the main pathogen detected (1.37%; 95% CI: 1.16-1.57%; n = 12 227), although only one sample exceeded the unsatisfactory threshold limit of 100 CFU/g. Enteric pathogens were less often detected, with a prevalence of 0.11% (95% CI: 0.05-0.17%; n = 11 538) and 0.02% (95% CI: 0.00-0.05%; n = 12 601) for STEC and Salmonella spp., respectively. Elevated levels (> 100 000 CFU/g) of presumptive B. cereus were found in 0.34% (95% CI: 0.18-0.51%; n = 4954) of cases, while coagulase-positive staphylococci were detected (> 100 CFU/g) in 0.26% (95% CI: 0.11-0.42%; n = 4169) of samples, with a maximum concentration of 190 CFU/g. The prevalence of L. monocytogenes fluctuated over time, varying from a minimum of 0.69% in 2022 to a maximum of 2.03% in 2017 and showing a seasonal effect, with an increased prevalence in June and from September to December. The major produce-pathogen combinations were L. monocytogenes and mushrooms (10.19%; 95% CI: 6.89-13.48%; n = 324) and head brassica (6.85%; 95% CI: 4.15-9.55%; n = 336); as well as STEC and legumes (0.47%; 95% CI: 0.00-1.39%; n = 213) and leafy brassica (0.40%; 95% CI: 0.00-1.17%; n = 252). This study provides valuable information for stakeholders, including farmers, distributors, retailers, and policymakers to be used in risk assessment.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100560"},"PeriodicalIF":2.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol of two Escherichia coli O157:H7 strains and a non-pathogenic surrogate E. coli in wheat grains and wheat milling operations using a lytic bacteriophage cocktail.","authors":"Amit Vikram, Kristen McCarty, Mary T Callahan, Joanne Sullivan, Alexander Sulakvelidze","doi":"10.1016/j.jfp.2025.100557","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100557","url":null,"abstract":"<p><p>Shiga toxin-producing Escherichia coli (STEC) contamination of wheat and flour has emerged as a significant public health risk and a major challenge for the milling industry. Several interventions such as chlorine, chlorine dioxide, ozone, lactic acid etc. have been evaluated and are used with variable success. Phage biocontrol has emerged as an effective strategy for controlling foodborne pathogens in various foods. This study examined the efficacy of phage biocontrol in mitigating Escherichia coli O157:H7 contamination in soft wheat milling operations. The findings demonstrated the efficacy of phage applications in reducing E. coli O157:H7 on wheat while maintaining flour quality and sensory attributes. Laboratory tests showed that the phage cocktail reduced E. coli O157:H7 populations by up to 1.3 log CFU/g within 4 hours on wheat grains, with reductions correlated to phage concentrations. In mill trials, tempering soft wheat with phage cocktail at three concentrations i.e. 1×10⁶ PFU/g, 2.5×10⁶ PFU/g and 1×10⁷ PFU/g resulted in significant reductions in populations of a non-pathogenic E. coli O88 strain, ranging from 1.4 to 3.1 log CFU/g, in a dose dependent manner. Tempering with phage reduced the E. coli populations throughout the milling operation. Two different tempering systems, the DAMPE system and the Bühler Turbolizer, were evaluated. Both systems achieved similar overall reductions, although the DAMPE system showed slightly higher efficacy than the Bühler Turbolizer after 6 hours tempering. Additionally, phage treatments reduced positive samples in straight-grade and patent flours by 15%-29%. Importantly, solvent retention capacity and hydration performance of phage-treated flour were unaffected, and sensory tests indicated no changes in flavor, texture, or appearance of baked products, including cookies and pancakes. These findings confirm that phage applications effectively reduce foodborne pathogens on wheat without compromising product quality.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100557"},"PeriodicalIF":2.1,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144266417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing Lead Levels in New York-Donated Venison.","authors":"Peter E Nye, Samantha Totoni, Karyn L Bischoff","doi":"10.1016/j.jfp.2025.100556","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100556","url":null,"abstract":"<p><p>Lead (Pb) hunting ammunition is known to fragment in target animals and result in Pb particles with small surface areas, which increase bioavailability and makes detection more difficult. Dietary exposure to Pb presents significant human health risks to vulnerable groups, especially children and pregnant women. Each year, 34 tons of venison are donated to New York State food banks and pantries without oversight of potential for Pb contamination. A project was launched in 2022 to sample donated venison and to determine if and at what concentration this meat might be contaminated with Pb. Tests for Pb were first conducted by medical radiography (X-ray) of 0.45 kg (1 lb) and 0.91 kg (2 lb) samples followed by subsampling for quantitative analysis using inductively coupled atomic plasma emission spectroscopy (ICP-AES). Among venison packages, 19% (11/ 59) were confirmed to contain metal fragments via radiography and/or Pb via ICP-AES. This includes seven packages with observable metal fragments via X-ray, three packages with suspected metal fragments via X-ray and detected via ICP-AES and one package with no radiographic evidence of Pb, but detected via ICP-AES. Pb concentrations among package sub-samples ranged from 0.38 - 135.7 mg/kg dry matter, representing a range from 1-617% of the FDA Interim Reference Level (IRL) of 2.2 µg Pb/day for children. Although some states routinely radiograph venison donated to food banks and pantries prior to distribution, New York State does not. Most New York State food pantries also do not post warnings of the potential for Pb contamination in donated game meat. In conclusion, there is a high likelihood that people consuming donated hunted meat in NY will consume lead, and the FDA IRL may be exceeded in a single bite.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100556"},"PeriodicalIF":2.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of UVC-Assisted Fenton Reaction Wash-Based System to Inactivate Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes on Cherry Tomatoes.","authors":"Xuetong Fan, Joshua B Gurtler, Jessica Baik, Christina M Garner, Bryan T Vinyard","doi":"10.1016/j.jfp.2025.100555","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100555","url":null,"abstract":"<p><p>The Fenton reaction results when hydrogen peroxide (H₂O₂) interacts with the ferrous ion, producing highly antimicrobial hydroxyl free radicals (·OH). In this study, a UVC-assisted Fenton reaction was tested against cocktails of Salmonella Typhimurium, E. coli O157:H7, and Listeria monocytogenes, inoculated onto cherry tomatoes. Inoculated cherry tomatoes were subjected to 2% H₂O₂ wash, 1 mM Fe³⁺ wash, or exposed to UVC during washing, and simultaneously in the following combinations: H₂O₂ + Fe³⁺, H₂O₂ + UVC, or H₂O₂ + Fe³⁺ + UVC for 2 min at ambient temperature (∼20°C). In addition, ·OH were measured using the methylene blue dye test, after combining Fenton reaction components in water. Results revealed that H₂O₂ alone, or in combination, reduced significantly higher populations of the three bacterial pathogens, when compared with water wash alone. The H₂O₂ + Fe³⁺ + UVC treatment inactivated 5.24±1.17 log CFU Salmonella/fruit, which was significantly (P<0.05) greater than those reduced by Fe³⁺ or UVC alone. However, the reduction was not statistically significant when compared to H₂O₂, H₂O₂ + Fe³⁺ or H₂O₂ + UVC. Similarly, the combinations did not inactivate significantly (P>0.05) more E. coli O157:H7 and L. monocytogenes than individual H₂O₂ or UVC treatments with reductions of 2.31-3.20 and 1.96-3.17 log CFU/fruit, respectively, although ·OH were produced in water during the H₂O₂ + Fe³⁺ + UVC treatments. Overall, our results demonstrate that UVC-assisted Fenton washing does not consistently exhibit advantages over individual H₂O₂ or UVC treatments during cherry tomato washing to inactivate foodborne pathogens, presumably due to inability of short-lived hydroxyl radicals to reach the bacteria on tomatoes.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100555"},"PeriodicalIF":2.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a test for the real-time fluorescence RNA targeted isothermal amplification to quickly identify Cronobacter species in powdered infant formula.","authors":"Ning Ding, Xiaona Tang, Fang Chen, Jiaqi Liang, Li Ling, Song Cheng, Lidan Ma, Yan Lu, Muyun Yuan, Jingwen Liu, Qing Liu, Wenrui Chen, Shan Huang, Furong Yang, Riqin Wu","doi":"10.1016/j.jfp.2025.100554","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100554","url":null,"abstract":"<p><p>Foodborne bacteria such as Cronobacter are opportunistic and have been connected to potentially fatal infections. The current work created a fast and sensitive RNA targeting amplification and detection system for Cronobacter spp., including enrichment, RNA isolation and detection by real-time RNA isothermal amplification, capable of detecting viable Cronobacter spp. in powdered infant formula (PIF) and other food products. Total RNA was extracted to optimize Simultaneous Amplification and Testing (SAT) reaction conditions including concentrations of primers, molecular beacon, Mg²⁺, dNTP, and NTP. The optimized SAT assay targeting 16s/23s rRNA was used to demonstrate the specificity, sensitivity of the detection assay. Seven Cronobacter sakazakii strains and 24 control strains were examined in comparison that of real time PCR (SN/T 1632.3) and ISO 22964.The SAT assay showed sensitivity with detection limit of 2 log10 CFU/ml without pre-enrichment, 2 log CFU/10ml with 4 hours enrichment and 2 log CFU/1000ml with 7 hours pre-enrichment (The sensitivity of real-time PCR are 3 log CFU/ml without pre-enrichment, log CFU/ml with 4 hours pre-enrichment and 2 log CFU/10ml with 8 hours pre-enrichment) The new developed assay could provide results in 4 hours including enrichment, which has been significantly shortened compare with real time PCR method with overnight enrichment. Moreover, SAT assay did not give false positive results when detecting dead C. sakazakii (7-2 log CFU/ml). In contrast, the real-time PCR assay exhibited a detection limit equivalent to that for detecting viable bacteria.The developed SAT assay, combined with enrichment, provide a rapid, sensitive and simple approach, has great potential in the detection of Cronobacter species in baby formula and other food goods.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100554"},"PeriodicalIF":2.1,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal Study of Environmental Factors Influencing Microbial Contamination in Alabama Ponds: Implications for Qualitative Risk Assessment","authors":"Zoila Chevez ,&nbsp;Elisa Tobar ,&nbsp;Daniel Weller ,&nbsp;Camila Rodrigues","doi":"10.1016/j.jfp.2025.100552","DOIUrl":"10.1016/j.jfp.2025.100552","url":null,"abstract":"<div><div>Agricultural water can be a source of microbial contamination for preharvest produce that has been linked to outbreaks and recalls. Over a two-year period, seven agricultural ponds were evaluated bimonthly. A total of 347 samples were tested for EHEC and <em>Salmonella</em> presence using PCR screening. Generic <em>E. coli</em> was quantified and ranged from 1.00 to 4.00 log₁₀ MPN/100 mL. <em>Salmonella</em> was detected in 6% of samples (21/347), and Kentucky was the most frequently isolated serotype. Public health concern serotypes, such as Newport and Hartford, were also isolated. EHEC biomarkers were detected in 83% (289/347) samples, with gene prevalence as follows: <em>hly</em> 68%, <em>fliC</em> 40%, <em>eaeA</em> 19%, <em>rfbE</em> 12%, <em>stx2</em> 10%, and <em>stx1</em> 4%. Microbial water quality and environmental factors were evaluated using conditional forest and regression analyses. Generic <em>E. coli</em> was negatively associated with the amount of developed land within 365 m of the sampling site and dissolved oxygen and positively associated with total rainfall during the 7 days preceding sampling and turbidity. <em>Salmonella</em> detection was positively associated with the amount of pasture within 30 m of the sampling site and total rainfall during the 7 days preceding sampling and negatively associated with rainfall 24 h prior to sampling. EHEC detection was positively associated with percentages of forest and wetland within a 304 m buffer from the sampling site, rainfall 48 h prior to sampling, and solar radiation, while negatively associated with percentages of developed land within a 91 m buffer from the sampling sites. This study provides baseline, longitudinal data on microbial hazards in Alabama agricultural ponds and informs growers on qualitative risk assessment of agricultural waterways.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 7","pages":"Article 100552"},"PeriodicalIF":2.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Camel Hide Gelatin in Donkey Hide Gelatin Based on Enzymatic Digestion by Ultra-performance Liquid Chromatography-Tandem Mass Spectrometry","authors":"Qiangsheng Xie ,&nbsp;Weijian Wang ,&nbsp;Tianyang Gao ,&nbsp;Feiyan Diao ,&nbsp;Wenyue Hu ,&nbsp;Xiaoyun Wu ,&nbsp;Silong Li ,&nbsp;Feng Shi ,&nbsp;Liping Gong ,&nbsp;Qiyan Li ,&nbsp;Guangxi Zhai","doi":"10.1016/j.jfp.2025.100553","DOIUrl":"10.1016/j.jfp.2025.100553","url":null,"abstract":"<div><div>The commercial value and diversified applications of donkey hide gelatin (DHG) have precipitated sophisticated adulteration practices, particularly with camel-hide gelatin substitutes. We present a mass spectrometry-based strategy to authenticate species-specific collagen peptides in commercial DHG. A new qualitative and quantitative methodology is constructed for camel hide gelatin(CHG) material analysis in DHG. It consists of enzyme-mediated digestion, ultra-high-performance liquid chromatography (UHPLC), and triple quadrupole mass spectrometry (MS/MS) with multireaction monitoring (MRM) mode. In brief, the samples were digested with trypsin and subjected to efficient and quick separation by UHPLC, and MS/MS performed CHG identification and determination analysis. Two peptides (Camel peptide A, CPA, and Camel peptide B, CPB) were identified as CHG-specific peptides. A specificity test was then used to verify these two peptides. The method performance showed that these two peptides could distinguish CHG from other animal hide gelatins. The CPA and CPB detection limits were 5 μg/kg and 3 μg/kg, respectively. LC-MS/MS analysis detected CPA and CPB in 4.5% of market-collected DHG samples (<em>n</em> = 157), confirming the prevalence of this emerging adulteration practice. Thus, the present protocol is a sensitive, accurate, quick, and suitable application of species-specific peptide biomarkers, ensuring the quality of DHG products and making them authentic and traceable to protect consumers from potential health risks and food frauds.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 7","pages":"Article 100553"},"PeriodicalIF":2.1,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profile of Salmonella isolates in bullfrog production: PFGE, MLST and antimicrobial resistance.","authors":"Evelyn Cristine da Silva, Sthéfany da Cunha Dias, Priscila Cristina Costa, Monique Ribeiro Tiba Casas, Ricardo Seiti Yamatogi, João Pessoa Araújo Junior, Marcus Vinícius Coutinho Cossi, Fábio Sossai Possebon","doi":"10.1016/j.jfp.2025.100550","DOIUrl":"https://doi.org/10.1016/j.jfp.2025.100550","url":null,"abstract":"<p><p>The continued development of bullfrog farming holds great potential for expansion. However, these animals serve as reservoirs for various pathogens, including Salmonella, posing a risk to food safety and public health. Therefore, the primary objective of this study was to identify potential sources of Salmonella contamination in a bullfrog production facility in Minas Gerais, Brazil, by characterizing isolates through Pulsed-Field Gel Electrophoresis (PFGE), Multilocus Sequence Typing (MLST), and antimicrobial resistance profiling. A total of eight Salmonella isolates were analyzed, comprising six isolates from bullfrog carcasses and two from breeding tanks. PFGE characterization revealed four distinct profiles, with a clonal relationship among isolates belonging to the same serovar, except for S. Newport, where the profile of the breeding tank isolate differed from that of the carcass isolates. MLST analysis identified three sequence types (ST): ST 32, ST 614, and ST 6855, corresponding to S. Infantis, S. Newport, and S. 6,8:i:-, respectively. Regarding antimicrobial resistance, one isolate was resistant to azithromycin, two were resistant to neomycin, and three were resistant to ciprofloxacin. All isolates were susceptible to cephalexin, meropenem, cefotaxime, ceftriaxone, ampicillin, tobramycin, and cephalothin. The presence of distinct PFGE profiles among isolates of the same serovar suggests multiple sources of pathogen contamination within the production chain, raising significant sanitary concerns. The identified sequence types (STs) are of public health relevance, highlighting the pathogenic potential of these isolates. While most isolates were susceptible to the tested antimicrobials, the detection of azithromycin resistance is particularly concerning. The combined PFGE and MLST data indicate potential cross-contamination within the production chain, emphasizing the need for stringent control measures.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100550"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Pathogenic Microorganisms in Fresh Produce Production: Lessons Learned When Plant Science Meets Food Safety","authors":"Xingchen Zhao ,&nbsp;Leo Van Overbeek ,&nbsp;Catherine M. Burgess ,&nbsp;Nicola Holden ,&nbsp;Fiona Brennan ,&nbsp;Gro S. Johannessen ,&nbsp;Ana Allende ,&nbsp;Monica Höfte ,&nbsp;Bart Cottyn ,&nbsp;Joël F. Pothier ,&nbsp;Adam Schikora ,&nbsp;Mieke Uyttendaele","doi":"10.1016/j.jfp.2025.100551","DOIUrl":"10.1016/j.jfp.2025.100551","url":null,"abstract":"<div><div>To enhance control of human pathogenic microorganisms in plant production systems, an EU COST Action (HUPLANTcontrol CA16110) was initiated, bringing together microbiologists in food, environmental, and plant microbial ecology. This article summarizes the outcomes of multiple workshops and the four main lessons learned: (i) many terminologies need further explanation to facilitate multidisciplinary communication on the behavior of human pathogens from preharvest plant production to postharvest food storage, (ii) the complexity of bacterial taxonomy pushes microbial hazard identification for greater resolution of characterization (to subspecies, or even strain level) needing a multimethod approach, (iii) hazard characterization should consider a range of factors to evaluate the weight of evidence for adverse health effects in humans, including strain pathogenicity, host susceptibility, and the impact of the plant, food, or human gut microbiome, (iv) a wide diversity of microorganisms in varying numbers and behaviors coexist in the plant microbiome, including good (beneficial for plant or human health), bad (established human or plant pathogens), or ugly (causing spoilage or opportunistic disease). In conclusion, active listening in communication and a multiperspective approach are the foundation for every successful conversation when plant science meets food safety.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"88 7","pages":"Article 100551"},"PeriodicalIF":2.1,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}