Journal of food protection最新文献

{"title":"Mechanistically Informed and Omics-Guided Essential Oil Applications for Food Preservation.","authors":"Mohammad Nazrul Islam Bhuiyan, Md Saidur Rahman, Md Mahfuzur Rahman","doi":"10.1016/j.jfp.2026.100806","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100806","url":null,"abstract":"<p><p>The growing demand for safe, sustainable, and clean-label food preservation strategies has accelerated interest in plant-derived essential oils (EOs) as natural antimicrobial agents. These chemically complex mixtures exhibit broad-spectrum activity against foodborne pathogens and spoilage microorganisms through multi-target mechanisms, including membrane disruption, metabolic interference, oxidative stress induction, and modulation of quorum sensing and biofilm formation. However, their practical application remains constrained by compositional variability, physicochemical instability, matrix-dependent efficacy, and sensory limitations. Recent advances in multi-omics technologies have shifted the understanding of EO-microbe interactions from descriptive observations to mechanistic, systems-level insights. Transcriptomic, proteomic, and metabolomic analyses reveal coordinated cellular responses, including stress adaptation, efflux activation, and metabolic reprogramming, providing a foundation for rational optimization of EO-based preservation strategies. In parallel, artificial intelligence (AI) and machine learning (ML) offer complementary tools for modeling complex interactions and predicting antimicrobial outcomes. However, their application remains limited by data heterogeneity, insufficient validation in real food systems, and challenges in interpretability. This review critically synthesized mechanistic, omics-driven, and AI-enabled approaches in EO-based food preservation, while addressing key translational barriers, including lack of standardization, regulatory complexity, and scalability. Integrating mechanistic biology with data-driven modeling provides a pathway toward more reliable and precision-oriented EO preservation systems aligned with sustainable food safety demands.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100806"},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of PCR-Based H5N1 Influenza Detection Methods in Milk from an Inter-laboratory Comparison Study Demonstrating Method-Dependent Sensitivity Variability.","authors":"Megan R Miller, Kirstin Frost, Emily L Smith, Yuhan Jin, Jodie Ulaszek, Matthew Kmet, Karina Hettwer, Anja Schlierf, Sarah M Nemser, Andriy Tkachenko, Laura B Goodman, Olgica Ceric, Kelli Almes, Christina Loiacono, Ravinder Reddy, Steffen Uhlig, Gregory H Tyson","doi":"10.1016/j.jfp.2026.100798","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100798","url":null,"abstract":"<p><p>Since March 2024, H5N1 highly pathogenic avian influenza (HPAI) has been detected in U.S. dairy cattle. Infectious virus shed in unpasteurized milk, a matrix not previously evaluated for H5N1 detection, makes real-time reverse transcription quantitative polymerase chain reaction (rRT-qPCR) assays critical for animal diagnostics and food safety through screening and early detection of milk samples. This interlaboratory comparison exercise evaluated the performance of PCR-based H5N1 assays across 45 participants from government, academic, and commercial laboratories using blinded milk samples spiked with an inactivated H5N1 virus culture at concentrations ranging from 85 to 170,000 genome copies/100 μL. Participants tested samples using their routine protocols. The rate of detection (ROD), Ct values, and levels of detection where 50% of the samples yielded a positive result (LOD₅₀) were analyzed with methods grouped by extraction and PCR kit combinations for sensitivity comparison. For high-concentration samples (17,000 and 170,000 copies/100 μL), ROD was ≥99%; however, detection decreased significantly at lower concentrations, with only 34% of participants reliably detecting samples at 1,700 copies/100 μL and 24% at 85 copies/100 μL. Eight distinct method types were used by participants, with some methods consistently detecting low levels of virus, and others having a 0% detection rate at low copy levels. This study reveals substantial variability in H5N1 detection capabilities across laboratories, with method selection impacting sensitivity. This finding has important implications for human and animal health surveillance and emergency response, highlighting the need for evaluation of protocols and quality control measures for H5N1 testing in milk.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100798"},"PeriodicalIF":2.8,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147856437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Secondary Colonizer Hypothesis: an Ecological View of Listeria monocytogenes Persistence in Food Processing Environments.","authors":"Jack Burnett, David Buckley, Dale Grinstead","doi":"10.1016/j.jfp.2026.100801","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100801","url":null,"abstract":"<p><p>Listeria monocytogenes demonstrates remarkable persistence in food processing environments, particularly in areas with established biofilm communities. This communication synthesizes emerging evidence for a novel ecological strategy whereby L. monocytogenes functions as a secondary colonizer, exploiting central voids created during Gram-negative bacterial biofilm dispersal. We propose the \"secondary colonizer hypothesis\" - that L. monocytogenes utilizes a sophisticated temporal succession strategy, colonizing biofilm voids characterized by steep oxygen gradients and phosphatidylethanolamine-rich debris from dispersed primary colonizers. This strategy employs specialized metabolic pathways including cobalamin-dependent catabolism of ethanolamine, propylene glycol, and glycerol while simultaneously utilizing ferric iron reduction for respiration. The metabolic coupling allows each bacterial cell to accumulate iron via ferritin-like protein storage, creating competitive advantages through localized limitation. Recent evidence identifies Veillonella as a key bridging species that may facilitate this ecological succession through metabolic complementarity. Notably, while L. monocytogenes co-occurs with Gram-positive bacteria, its metabolic specialization for ethanolamine and iron scavenging suggests a preferential adaptation to the nutrient-dense voids of dispersed Gram-negative biofilms. The implications for food safety protocols, particularly cleaning chemical formulations and ecological monitoring strategies, warrant immediate attention.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100801"},"PeriodicalIF":2.8,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct-Fed Microbials in Diets of Market Pigs Did Not Impact Salmonella Levels, Shiga Toxin-Producing Escherichia coli, Growth Performance, and Carcass Characteristics.","authors":"Jimeng Bai, Macie E Reeb, Michael D Tokach, Jordan T Gebhardt, Jason C Woodworth, Robert D Goodband, Joel M DeRouchey, Jessie Vipham, Qing Kang, John W Schmidt, Dayna M Brichta-Harhay, Joseph M Bosilevac, Morgan Miller, Sara E Gragg","doi":"10.1016/j.jfp.2026.100805","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100805","url":null,"abstract":"<p><p>Salmonella enterica and Shiga toxin-producing Escherichia coli (STEC) have been associated with the gastrointestinal tracts of growing-finishing swine. Identification of pre-harvest pathogen mitigations is imperative. The probiotics BIOPLUS 2B and Probicon L28 were evaluated as direct-fed microbials to 1) reduce Salmonella and STEC in the lymph nodes and feces of commercial growing-finishing pigs, and 2) improve growth performance and carcass characteristics. Market pigs (n=650) were fed a standard corn-soybean meal finishing diet according to the following treatments: Probicon L28 at 1.0×10⁶ CFU/head/day (Probicon); BIOPLUS® 2B at 3.0×10⁹ CFU/head/day (BIOPLUS 2B); and a control (Control). Fecal samples, boot covers, and chew ropes (tied to pen) were collected upon arrival (baseline), 6 wk post-placement, and prior to loadout (13 wk post-placement). Superficial inguinal lymph nodes (SILNs) were collected at the abattoir. Salmonella and STEC occurrences were determined for all samples. Pen (∼21-day intervals) and individual weights were used to assess growth performance. Carcasses were evaluated by hot carcass weight, loin depth, backfat, and percentage lean. Salmonella prevalence in group 1 and group 2 market hogs was 0.7% and 2.8% in feces, and 0.8% and 0% in SILNs, respectively. STEC O111 was infrequently detected (n=5 samples), and E. coli O157:H7 was not detected. The impacts of BIOPLUS® 2B and Probicon L28 on the prevalences of STEC genes (stx and eae) and STEC serogroups O26, O121, O45, O103, and O145) in feces, boot covers, chew ropes, and SILNs of market pigs were not different (P>0.05). Overall average daily gain, average daily feed intake, gain:feed ratio, and carcass traits did not differ (P>0.10) between treatments.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100805"},"PeriodicalIF":2.8,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine Nematodes in Seafood: A Review of Food Safety Concerns, Advanced Detection Techniques, and Mitigation Strategies.","authors":"Xiulian Ye, Jiaxu Wang, Jianwei Huang, Huarong Hong","doi":"10.1016/j.jfp.2026.100803","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100803","url":null,"abstract":"<p><p>Marine nematodes are important food-borne parasites infecting many species of marine fish and cephalopods. This review provides a comprehensive discussion of the food safety concerns associated with marine nematodes in seafood, along with emerging methods for their detection. The potential prevention strategies for these seafood-borne parasites were also explored. According to the results, anisakiasis is the most serious nematode disease humans acquire from marine fish, chiefly due to Anisakis simplex larvae and, to a lesser degree, Pseudoterranova decipiens. Several analytical methods are employed for the detection of marine nematodes in seafood, each characterized by its own set of advantages and drawbacks. While traditional visual and digestion-based methods are still employed, newer techniques like hyperspectral imaging, and molecular analysis are advancing the field. These innovations provide greater sensitivity, automation, and the ability to identify parasites at the species level. The safety and quality of seafood products are ensured through the application of methods including freezing, salting, pulsed electric fields and high hydrostatic pressure. The effectiveness of these methods depends on the processing technique, nematode species, and parasite's developmental stage. This review presents findings pertinent to seafood safety that will be of significant interest to both the research community and the industrial sector.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100803"},"PeriodicalIF":2.8,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and Simultaneous Detection of Five Classical Staphylococcus aureus Enterotoxin Genes via Multiplex Melting Curve Analysis Using Self-Quenching Primers.","authors":"Jinhui Liu, Qi Wang, Xuhao Wang, Haonan Qin, Yani Fang","doi":"10.1016/j.jfp.2026.100804","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100804","url":null,"abstract":"<p><p>Staphylococcus aureus is a ubiquitous foodborne pathogen whose pathogenicity is primarily driven by staphylococcal enterotoxins (SEs), necessitating rapid and reliable screening methods to prevent disease outbreaks. Current detection techniques, however, are often limited by high costs, post-amplification contamination risks, or labor-intensive protocols. To address these challenges, this study developed a novel, closed-tube multiplex melting curve assay utilizing self-quenching primers for the simultaneous detection of the five classical enterotoxin genes. The assay successfully differentiated all five target genes within a single reaction based on distinct melting temperatures and achieved a limit of detection of 10^-2 ng/μL, demonstrating 100% analytical specificity with no cross-reactivity against a panel of 12 non-target bacterial strains. Verification using 171 S. aureus strains isolated from milk samples revealed an overall enterotoxin prevalence of 47.95%, with SEA (19.30%) and SEE (12.28%) being the most predominant genotypes. Compared to conventional PCR, the proposed method demonstrated 100% specificity and 97.62% sensitivity, offering a robust, cost-effective, and high-throughput tool for the routine surveillance of enterotoxigenic S. aureus in the dairy industry.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100804"},"PeriodicalIF":2.8,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Machine Learning Framework for Meat Safety Risk Assessment with Cross-Domain Data Fusion.","authors":"Liying Wu, Yanna Ke, Yiming Huang, Xiaosheng Wang, Chuanhai Ding, Xin Wang, Yu Ge","doi":"10.1016/j.jfp.2026.100802","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100802","url":null,"abstract":"<p><p>The escalating complexity of global meat supply chains and rising consumption levels heighten public health risks, particularly in high-volume regions. To address this, we developed a novel risk assessment framework integrating multi-source heterogeneous data from seven key origins. The framework implements LLM-enhanced data governance, a Delphi-AHP dual-dimensional indicator system, and an optimized Random Forest model, achieving predictive accuracy between 88.25% and 99.24%. Analysis identified pork as the highest-risk meat category, fresh meat as the riskiest processing type, and a shift since 2022 toward biological hazards, especially pathogens, as the dominant risk over chemical contaminants. This data-driven tool enables proactive, targeted risk management and supports regulatory decision-making for enhanced meat safety.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100802"},"PeriodicalIF":2.8,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Listeriosis outbreaks in Italy in 2022-2023: management and source identification.","authors":"M Torresi, L Bolzoni, A Chiaverini, G Centorotola, F Guidi, C Cammà, S Morabito, G Scavia, A Fiore, R Gaglione, R Lena, S Pilati, S Pongolini, V Cibin, P Di Taranto, V Russini, A Amorena, F Pomilio","doi":"10.1016/j.jfp.2026.100797","DOIUrl":"https://doi.org/10.1016/j.jfp.2026.100797","url":null,"abstract":"<p><p>Implementation of effective teams that investigate listeriosis outbreaks is important to both limit the scope of outbreaks and to identify root causes and outbreak sources. This paper describes activities of the Italian outbreak investigation team, highlighting cross-sectoral collaboration in listeriosis surveillance and presenting a framework to strengthen national outbreak preparedness and response, using four Listeria monocytogenes (Lm) outbreaks investigated in Italy between 2022 and 2023. These outbreaks involved strains belonging to clonal complexes (CC) CC1, CC8, and CC155. Although CC1 is globally recognized as a hyper-virulent lineage predominantly associated with invasive listeriosis cases, CC8 and CC155 have been increasingly implicated in outbreaks reported in several European countries in recent years. Notably, the CC155 outbreak investigated in 2022 represented one of the largest outbreaks reported in Italy (101 cases). The implicated foodborne vehicles were meat products (chicken and turkey frankfurter, mortadella and porchetta) and vegetables (black olive) in line with EFSA's 2023-2024 zoonoses reports, which identified pork and pork-derived products as the primary reservoirs for Lm transmission in Europe. Advanced molecular epidemiology was instrumental in outbreak detection and source attribution. cgMLST analysis facilitated large-scale surveillance and rapid comparisons, while single nucleotide polymorphism (SNPs) analysis enabled high-resolution phylogenetic characterization and discrimination of outbreak-related isolates improving the robustness investigations. The combination of a top-down regulatory framework with a bottom-up structure allowing rapid response and local-level outbreak detection proved critical for timely intervention. Future efforts should focus on harmonizing One Health surveillance system through centralized data repositories, enhanced communication tools, and increased engagement of regional and local competent authorities.</p>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":" ","pages":"100797"},"PeriodicalIF":2.8,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147816243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Validation of an Evaporative, Low-Flammability, and Low-Moisture Antimicrobial Liquid for Dry Cleaning and Sanitization of Food Processing Equipment","authors":"Shihyu Chuang,&nbsp;Galaxie Story,&nbsp;Lynne McLandsborough","doi":"10.1016/j.jfp.2026.100716","DOIUrl":"10.1016/j.jfp.2026.100716","url":null,"abstract":"<div><div>Environmentally persistent organisms such as <em>Salmonella</em> spp. and <em>Cronobacter</em> spp. have caused several high-profile outbreaks associated with the consumption of low-moisture foods. Sanitary operations in dry food-processing environments are challenging as excess moisture elevates the risk of microbial harborage and transfer. In this work, the evaporation kinetics of selected polar and nonpolar solvents was characterized with dynamic vapor sorption. A low-moisture, volatile antimicrobial liquid was formulated using 3% v/v water-in-cyclomethicone emulsion as the carrier for 200 mM acetic acid, which had a closed-cup flashpoint of 80 °C and a rate of bacterial-killing by greater than 7 log CFU/coupon in 10 min at 22 °C against <em>Salmonella</em> and <em>Cronobacter</em> cocktails desiccated (33% ERH) on a stainless-steel surface. Hand-cleaning validation involving inoculated-and-equilibrated milk films suggested that decontamination of the underlying substratum required prior, extensive scrubbing. Collectively, the developed system showed potential as an alternative to alcohol-based agents for dry cleaning and sanitization.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"89 4","pages":"Article 100716"},"PeriodicalIF":2.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the use of Safety-Assessed Bacteriocin-Producing Enterococci as Starters for Production of Soy Yoghurt Analogues","authors":"Belay Tilahun Tadesse ,&nbsp;Jennifer C. Molloy ,&nbsp;Shuangqing Zhao ,&nbsp;Liuyan Gu ,&nbsp;Carsten Jers ,&nbsp;Ivan Mijakovic ,&nbsp;Christian Solem","doi":"10.1016/j.jfp.2026.100715","DOIUrl":"10.1016/j.jfp.2026.100715","url":null,"abstract":"<div><div>Plant-based yoghurt analogs have gained significant popularity recently. Key driving factors are lactose intolerance and the perception that plant-based foods are more sustainable than dairy alternatives. When preparing plant-based yoghurt analogs, often conventional yoghurt cultures are used, despite the fact that plant-based substrates differ significantly from milk. Enterococci are known for their broad carbohydrate utilization repertoire, and many strains are considered safe and are used as probiotics. In this study, we explored the potential of enterococci for fermenting soymilk. Out of four strains tested, <em>Enterococcus faecium</em> BT0194, <em>Enterococcus lactis</em> B0167_2, <em>E. lactis</em> BT0173_2, and <em>E. lactis</em> CS4674, three strains acidified plain soymilk to a pH below 4.7 using an initial inoculum of 10⁶ cells/ml (standard inoculum when preparing yoghurt). <em>Enterococcus</em> is renowned for producing bacteriocins; all strains harbored multiple bacteriocin genes. Thus, we investigated whether the four strains could confer a bioprotective effect, and indeed, a strong antimicrobial effect was observed against the tested pathogens. Three strains demonstrated α-galactosidase activity, which is necessary for degrading the indigestible and flatulence−inducing α-galactosides raffinose, stachyose, and verbascose, which are undesirable in soymilk. Additionally, all tested strains had the ability to degrade phytic acid, an unwanted antinutrient found in many plant-based foods, including soymilk. In conclusion, our results demonstrate that the <em>Enterococcus</em> strains tested exhibit considerable potential for use in plant-based fermentations, due to efficient acidification capacity and a capacity to degrade phytic acid in soymilk.</div></div>","PeriodicalId":15903,"journal":{"name":"Journal of food protection","volume":"89 4","pages":"Article 100715"},"PeriodicalIF":2.8,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146137347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}