Journal of Biomaterials Applications最新文献

{"title":"Characterization and hemocompatibility of poly (N-acryloyl-L-tryptophan) nanoparticles as targeting delivery carriers for vinblastine.","authors":"Zishan Zhou, Hongquan Tao, Haocheng Yang, Ao Duan, Jiahui Yu, Yixin Chen, Yongyan Zhu, Quanhong Zhu","doi":"10.1177/08853282261422124","DOIUrl":"10.1177/08853282261422124","url":null,"abstract":"<p><p>Favorable biocompatibility is essential to biomaterials, and natural amino acids are recognized as the promising building block of polymers due to their non-toxicity and tunable side chains. We prepared polymeric nanoparticles (NPs) using N-acryloyl-L-tryptophan monomer by precipitation polymerization, and modified with polyethylene glycol and folate (PEG-FA) to improve the solubility and target folate-receptors (FR) overexpressed tumor tissues. Serving as drug carriers for vinblastine (VBL), NPs-PEG-FA with about 212.4 nm had the drug loading of VBL of 6.65 ± 0.41% after co-incubating for 1 h and showed sustained-release in pH 7.4 PBS, in which 99.87 ± 1.00% of VBL was released from NPs-PEG-FA during 72 h. Furthermore, NPs-PEG-FA was more efficiently taken up by FR positive Hela cells compared with NPs-PEG, which signified folate could enhance the internalization of NPs-PEG-FA into FR over-expressed cells. And NPs-PEG-FA began to enter Hela cells in large quantities from 3 h onwards, meanwhile the released drug increased more quickly in the first 3 h, which indicated most of the drugs would be released after entering tumor cells. More importantly, NPs-PEG-FA had good biocompatibility to L929 mouse fibroblast cells and exhibited hemocompatibility via the assays of hemolysis, antithrombogenicity, coagulation activation and platelet activation. NPs-PEG-FA could serve as drug carriers for delivering drugs into FR positive tumor cells.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"537-553"},"PeriodicalIF":2.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147498879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revitalizing bioinert PCL: A biomimetic 3D-Printed gradient-pore scaffold with CSMA hydrogel-mediated BMP-2/QK delivery for vascularized bone regeneration.","authors":"Zekun Zhang, Chunjiao Huang, Wenbin Hu, Shikun Wu","doi":"10.1177/08853282261492945","DOIUrl":"https://doi.org/10.1177/08853282261492945","url":null,"abstract":"<p><p>Critical-sized bone defects exceed bone's regenerative capacity and require space-maintaining implants that support vascular and bone-tissue ingrowth. Three-dimensional (3D)-printed polycaprolactone (PCL) scaffolds are widely investigated for bone repair owing to controllable geometry, interconnected macropores, and structural stability. However, uniform-pore designs cannot recapitulate heterogeneous bone porosity, while hydrophobic PCL has limited bioactivity. Inspired by the transition from dense cortical to porous cancellous bone, we designed a stepwise gradient-pore PCL framework combining compact regions for structural support with progressively more open regions, providing multiple cell- and tissue-relevant environments. An in situ photocrosslinked methacrylated chondroitin sulfate (CSMA) hydrogel containing bone morphogenetic protein-2 (BMP-2) and the vascular endothelial growth factor-mimetic QK peptide was formed within the printed pores as a hydrated delivery phase. The scaffold retained sequential dense, intermediate, and loose regions; CSMA improved wettability; and PCL confinement slowed the release of both factors relative to hydrogel alone, with QK reaching approximately 80% cumulative release by day 14 and BMP-2 approximately 65% by day 28. Relative to PCL and PCL-CSMA, PCL-CSMA-QK-BMP enhanced endothelial responses, rat bone marrow mesenchymal stem cell proliferation and migration, and osteogenic differentiation and mineralization. Single-factor comparisons supported QK-related endothelial and BMP-2-related osteogenic effects. In rat calvarial defects, PCL-CSMA-QK-BMP increased bone volume fraction, trabecular number, bone mineral density, collagen-rich matrix deposition, and osteogenic and angiogenesis-associated marker expression at 4 and 8 weeks relative to PCL and PCL-CSMA. These findings support coupling graded macroporosity with a hydrated delivery phase for vascularized bone regeneration.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261492945"},"PeriodicalIF":2.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149080795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential of Manganese (Mn) and Zinc (Zn) ion-doped bioactive glass-based fish collagen-carrageenan biomatrix in tissue remodeling of burn wounds.","authors":"Samit Kumar Nandi","doi":"10.1177/08853282261494282","DOIUrl":"https://doi.org/10.1177/08853282261494282","url":null,"abstract":"<p><p>Burn injuries are a major global public health crisis and pose significant problems with limited treatment options due to poor angiogenesis and antimicrobial resistance. To overcome these challenges, a new composite comprising fish collagen (waste-derived), carrageenan, and bioactive glass doped with Zn and/or Mn was developed. FTIR spectroscopy confirmed the presence of functional groups, while XRD patterns indicate the degree of crystallinity in the formulations. The composite hydrogels are highly hemocompatible, with a hemolysis index of <1%. SEM and EDX analyses revealed a mesoporous surface in composite films containing bioactive glass nanoparticles (Zn and/or Mn). Dual-doped bioactive glass enhances the formulation's antimicrobial activity against both Gram-positive and Gram-negative bacteria. The cell viability assays (MTT and FDA) confirm the non-toxicity of the composite gels on human dermal fibroblasts. <i>In vivo</i> assessment in an animal burn wound model with dual doping of Mn and Zn shows accelerated wound contraction, consistent fibroblast proliferation, minimal inflammation, advanced collagen organization, and epithelial regeneration. The results are further correlated with elastin production and immunohistochemical analysis for CD31 and collagen type-1, inferring promising wound-healing activity. This research showcases a smart, innovative, and powerful approach to developing advanced hydrogel dressings for treating burn wounds and complicated skin injuries.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261494282"},"PeriodicalIF":2.4,"publicationDate":"2026-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149074821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan hydrogel loaded with mesenchymal stem cell-derived exosomes significantly accelerated wound closure and enhanced tissue regeneration in a full thickness porcine skin defect model.","authors":"Guanghui Cui, Jinjin Yan, Peng Cui, Xudong Zheng, Huiqin Wang, Lei Zhang, Yanmin Zhang, Tianshi Li","doi":"10.1177/08853282261483994","DOIUrl":"https://doi.org/10.1177/08853282261483994","url":null,"abstract":"<p><p>There is an urgent need for more effective wound healing therapies, particularly wound dressings that not only promote wound closure and reduce scar formation but also retain antibacterial properties. Chitosan, a natural copolymer with inherent hemostatic and antibacterial properties, and mesenchymal stem cell derived exosomes, which promote rapid re-epithelialization, collagen maturation, and scar reduction, were integrated into a single biofunctional dressing. This study evaluated the therapeutic efficacy and underlying mechanisms of this combined approach using a porcine full thickness skin excision model. <i>In vitro</i>, chitosan hydrogel effectively inhibited the growth of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> across various inoculation concentrations. Exosome treatment significantly suppressed the proliferation and migration of human dermal fibroblasts (HDFs), downregulated mRNA expression of collagen types I and III and α-smooth muscle actin, while upregulating basic fibroblast growth factor and elastin. Furthermore, exosomes markedly increased vascular endothelial growth factor and insulin like growth factor levels, while decreasing hepatocyte growth factor production compared to untreated HDF controls. Exosome treatment also attenuated lipopolysaccharide (LPS) induced inflammatory responses in HDFs, as evidenced by reduced interleukin-1β (IL-1β), IL-6, and IL-8 secretion. Similarly, chitosan hydrogel reduced IL-1β, IL-6, IL-8, and tumor necrosis factor-α expression while enhancing IL-10 levels in LPS-stimulated peripheral blood mononuclear cells. <i>In vivo,</i> the combination of chitosan hydrogel and high-concentration exosomes significantly accelerated wound closure, enhanced epithelialization, minimized wound contraction, and prevented infection. Histological analysis revealed more mature and well developed epidermal and dermal structures, increased epithelial thickness, reduced myofibroblast percentage and inflammatory cell infiltration, enhanced angiogenesis, and greater type I collagen deposition in treated wounds. Collectively, these findings demonstrate that the chitosan-exosome composite harnesses natural antibacterial activity, inflammation modulation ability and regenerative capacity, establishing it as a highly promising biomaterial dressing for promoting cutaneous wound healing.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261483994"},"PeriodicalIF":2.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148880572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-performance organic-inorganic composite bone cement enabled by a Sr-doped alginate-phosphate ternary system.","authors":"Shuangying Tang, Qianjiang Liu, Yu Fu, Lihang Jiang, Wenjing Liu, Shuai Wu, Jiaqi Chen, Si Chen, Xu Wang, Chunsheng Qu","doi":"10.1177/08853282261428851","DOIUrl":"10.1177/08853282261428851","url":null,"abstract":"<p><p>Conventional calcium sulfate bone cements suffer from rapid degradation, poor anti-washout property, and inadequate bioactivity. To overcome these limitations, we developed an injectable bone cement based on a novel ternary synergistic system of Sr-doped alginate-phosphate (SA/Sr/PO₄^3-) and hydrothermally synthesized α-calcium sulfate hemihydrate (α-CSH). This design leverages the rapid physical crosslinking between Ca²⁺ from α-CSH and the guluronic acid/mannuronic acid blocks of sodium alginate, resulting in a reinforced network with enhanced initial anti-washout property and a wet compressive strength of 2-12 MPa. Simultaneously, the alginate matrix induces the in situ mineralization of Ca²⁺ and PO₄^3- ions, forming a calcium-deficient hydroxyapatite (CDHA) precursor that decelerates the degradation rate (18% in PBS over 60 days) to match new bone formation. In addition, strontium ions (Sr²⁺) sustainably released from the material promote in vitro osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). This work presents a novel composite cement that successfully integrates injectability, robust mechanical properties, controllable degradation, and osteoinductive capability.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"402-416"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147270939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Se nanoparticles-coated, PLGA-based spheres for biomedical applications: Cytotoxicity, genotoxicity, oxidative stress, biodistribution, and scintigraphic study.","authors":"Magdalena Stevanović, Jana Nunić, Metka Filipič, Giuseppe Digilio, Eirini Fragogeorgi, George Loudos","doi":"10.1177/08853282261422858","DOIUrl":"10.1177/08853282261422858","url":null,"abstract":"<p><p>Conventional approaches to prevent and treat diseases, particularly liver disorders, often fall short, highlighting the urgent need for innovative strategies and materials in RNA therapeutics and genetic drug delivery. This study investigates the synthesis, characterization, and biological evaluation of poly (DL-lactide-co-glycolide) (PLGA) spherical particles as a novel drug delivery system for selenium nanoparticles (SeNp), presenting a promising (PLGA/SeNp) platform for enhancing the efficacy of genetic therapies aimed at liver diseases. We assessed the effects of PLGA/SeNp nanoparticles <i>in vitro</i> using human hepatoma cell lines (HepG2 cells), focusing on (i) cell viability, (ii) intracellular reactive oxygen species (ROS) generation, and (iii) genotoxic response. The findings indicated that PLGA/SeNp nanoparticles maintained cell viability, exhibited minimal ROS generation, and demonstrated low genotoxicity, underscoring their biocompatibility for therapeutic applications. Furthermore, this study explored the <i>in vivo</i> biodistribution and pharmacokinetics of PLGA and PLGA/SeNp particles through non-invasive dynamic imaging techniques. By radiolabeling with technetium-99m (Tc^99m), we conducted scintigraphic imaging to analyze biodistribution. Our <i>in vivo</i> results revealed significant differences in the biodistribution profiles of PLGA and PLGA/SeNp formulations at 24 h post-injection, with PLGA/SeNp showing enhanced hepatic, splenic, and pulmonary uptake compared to PLGA. These findings emphasize the unique pharmacokinetic properties of the PLGA/SeNp system, presenting a viable option for RNA-based therapeutics in liver disease management.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"431-446"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-temperature interactions in gamma-irradiated tendon allografts: A multidimensional analysis.","authors":"Yanlin Wan, Dayong Liu, Xiaolong Wang, Limin Wang, Jingyu Zhang, Yongcheng Hu","doi":"10.1177/08853282261426964","DOIUrl":"10.1177/08853282261426964","url":null,"abstract":"<p><p>This study evaluated the impact of cryoprotection temperature on the structural and biomechanical properties of gamma-irradiated human tendon allografts. Tendons were irradiated at 15 kGy or 25 kGy under three temperature conditions: -70°C, 0°C, or room temperature (RT). Structural integrity was assessed histologically and biochemically, while biomechanical properties were measured via tensile testing. Tendons irradiated at RT exhibited severe collagen disorganization and cellular loss, whereas those cryoprotected at -70°C retained aligned collagen structure with minimal disruption. Biomechanically, the -70°C groups showed significantly higher maximum stress than RT groups at both irradiation doses. Increasing irradiation dose exacerbated structural and mechanical degradation, but these effects were substantially mitigated by cryoprotection at -70°C. Thus, low-temperature protection during gamma irradiation is crucial for preserving the integrity of tendon allografts.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"336-348"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146201677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of chemical and physical cross-linking strategies for CMCs/Gelatin/PVA wound dressings: Balancing mechanical strength with biocompatibility.","authors":"Sahar Molzemi, Sepehr Zamani, Naimeh Mahheidari, Majid Salehi","doi":"10.1177/08853282261429351","DOIUrl":"10.1177/08853282261429351","url":null,"abstract":"<p><p>In skin tissue engineering, the cross-linking of wound dressings is a critical step as it enhances mechanical strength. This improved strength regulates the release of incorporated components and determines the dressing's replacement schedule. Therefore, due to the lack of comparative knowledge for the cross-linking of carboxymethyl chitosan, gelatin, and polyvinyl alcohol (CMCs-Gel-PVA) wound dressings, this study was designed a systematic comparison of five distinct strategies: chemical cross-linkers-glutaraldehyde (GLU), EDC/NHS, citric acid (CA), and succinic acid (SA)-alongside a physical freeze-thaw method. Cross-linked wound dressings were fabricated and systematically characterized in terms of surface morphology (SEM), chemical bonding (FTIR), mechanical strength, porosity, biodegradability, fluid absorption, blood compatibility, cytotoxicity, and anti-inflammatory activity. The scaffolds exhibited interconnected pores ranging from 32.07 to 89.12 µm. The non-cross-linked sample showed higher porosity, liquid absorption, and degradation rate (90.33 ± 5.17%), which was attributed to its lower mechanical strength. All samples demonstrated good biocompatibility with no significant cytotoxic effects on 3T3 fibroblasts. Tensile strength values ranged from 0.15 to 1.92 MPa. Glutaraldehyde-crosslinked scaffolds displayed superior mechanical properties suitable for load-bearing wound applications, despite a slight reduction in cell viability (92.59 ± 3.19%), which remained above the accepted clinical biocompatibility threshold. These scaffolds also showed enhanced coagulation activity, whereas citric acid-crosslinked samples exhibited anticoagulant behavior and superior anti-inflammatory potential. In conclusion, this comparative study demonstrates that the choice of cross-linker dictates key functional properties of CMCs-Gel-PVA scaffolds, allowing for the strategic tuning of mechanical strength, degradation rate, hemostatic activity, and anti-inflammatory potential. These tailored <i>in vitro</i> characteristics support their further investigation as candidate materials for advanced wound dressing applications. The successful <i>in vivo</i> validation of these findings remains a critical next step to fully ascertain the translational potential of this biomaterial platform.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"363-386"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147306237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hydrothermal treatment time on the release kinetics of Ni²⁺ and Zn²⁺, corrosion behavior and biological properties of Zn-supported Ni-Ti-O nanopores coatings.","authors":"Zhiyan Lv, Yuehua Huang, Zhijian Li, Hailing Lao","doi":"10.1177/08853282261424660","DOIUrl":"10.1177/08853282261424660","url":null,"abstract":"<p><p>Bone implant materials with excellent corrosion resistance, antibacterial property, and controlled ion release kinetics are crucial for their clinical application. In this study, Zn-supported Ni-Ti-O nanopores (NPs) coating was prepared on the surface of nickel-titanium (NiTi) implants by anodization and hydrothermal treatment (HT), the effects of HT time on coatings structure, antibacterial property, corrosion resistance, Ni²⁺ and Zn²⁺ release behavior and biocompatibility were studied. The results indicate that with the extension of HT time, the coating thickness increases, while the diameters of the NPs decrease. After 3 h of HT, the average diameter of the NPs is 42.52 nm, which is about 1/2 of that after anodization (86.63 nm). Furthermore, our experimental results show that with the extension of HT time, the corrosion resistance is improved, and the corrosion current density of the sample NP-30-Zn-3h is 3.214 × 10^-8 A⋅cm^-2, which is one order of magnitude lower than that of the NiTi substrate (7.529 × 10^-7 A⋅cm^-2). In addition, Zn-supported Ni-Ti-O NPs coatings exhibit excellent antibacterial property and biocompatibility, especially the sample NP-30-Zn-3h. Interestingly, with the extension of HT time, the release of Ni²⁺ and Zn²⁺ is inhibited, and Zn²⁺ concentration released from the sample NP-30-Zn-3h is about 3.65 mg/L at days 7 and 14, which is close to the reported osteoblast-stimulating concentration of 3.53 mg/L.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"325-335"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet light-functionalized TiO₂ nanotubes for enhancing antibacterial properties and soft tissue integration of titanium implants.","authors":"Wen Han, Yijiao Yuan, Yurong Chen, Wei Wang, Chunhui Lu","doi":"10.1177/08853282261420579","DOIUrl":"10.1177/08853282261420579","url":null,"abstract":"<p><p>Peri-implantitis, a leading cause of dental implant failure, is primarily attributed to suboptimal soft tissue sealing at the implant-tissue interface, which facilitates bacterial colonization and subsequent inflammatory responses. Surface modifications on titanium can expedite good soft tissue sealing and antibacterial action. We engineer titanium surfaces with ultraviolet light-functionalized titanium dioxide nanotubes (UV-TNTs) to achieve good soft tissue sealing and antibacterial effect. Surface properties were characterized via scanning electron microscopy (SEM), surface roughness analysis, wettability assessment and methylene blue (MB) degradation. Antibacterial performance was quantified using <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>) viability assays. Soft tissue integration was evaluated through fibroblast proliferation assays and immunofluorescence staining of vinculin and collagen type I. The UV-TNTs demonstrated significantly enhanced antibacterial efficacy and promoted fibroblast proliferation, adhesion and collagen deposition to improve soft tissue sealing. This surface modification strategy offers a promising approach for enhancing implant biocompatibility and long-term stability, offering useful references for advanced implant design and fabrication.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"447-459"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146179741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}