Journal of Biomaterials Applications最新文献

{"title":"Biodegradation study in FBS media of polycaprolactone patch as a potential prenatal treatment for myelomeningocele.","authors":"K Benabdderrahmane, J Stirnemann, S Ramtani, C Falentin-Daudré","doi":"10.1177/08853282251316894","DOIUrl":"10.1177/08853282251316894","url":null,"abstract":"<p><p>Myelomeningocele (MMC) is a congenital defect of the spine characterized by meningeal and spinal cord protrusion through open vertebral archs, and its exposure to the amniotic fluid. Given that the progression of neuronal loss begins early in fetal life, an early coverage of the defect is required to improve the neurological outcomes. Several studies have proposed patches as an alternative to full surgical repair, to achieve an early protection of the spine and possibly reduce the rate of complications of current prenatal surgical procedures. In our previous work, we developed a biocompatible, watertight and biodegradable patch to improve in utero MMC repair. This patch offers an anti-adhesive internal surface to prevent adhesion to spinal cord tissue, and a bioactive external surface to promote tissue coverage. The aim of this study is to assess the patch's in vitro degradation in an amniotic-fluid-like medium and investigate the surface functionalization effect, to understand its mechanism and predict the patch's behavior over time. The study was carried out for 24 weeks in FBS medium and after each period the samples were characterized by differential scanning calorimetry, scanning electron microscopy, steric exclusion chromatography, toluidine blue assay and contact angle measurement. The results revealed a progression of PCL hydrolysis over time, characterized by a decrease in molar mass and evidence of erosion as observed by SEM. Furthermore, this process appears to be accelerated by ozonation, compared to surface functionalization without ozonation. The latter can be considered as the most suitable technique to preserve the patch structure over time, while benefiting from the grafting polymers properties during the first weeks of implantation.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1097-1114"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanobiological enhancement of electrospun PCL/nHA membranes for guided tissue regeneration applications.","authors":"Niki Dadgari, Hamidreza Fotoukian, Masoumeh Haghbin Nazarpak, Mehran Solati-Hashjin","doi":"10.1177/08853282241312285","DOIUrl":"10.1177/08853282241312285","url":null,"abstract":"<p><p>This study aims to investigate the effects of adding nano-hydroxyapatite (nHA) to electrospun polycaprolactone (PCL) membranes for use in dental root regeneration. Porous membranes containing varying amounts of nHA (0, 1, 1.5, and 2.5 wt%) were fabricated using the electrospinning method. The physicochemical, mechanical, and biological properties of the membranes were evaluated. The synthesized nHA particles had an average size of 52 nm. Electrospun membranes exhibited uniform fibrous morphology with porosities ranging from 56% to 86%. Cyclic thermal stress (5°C-50°C) improved the mechanical properties of the composite membranes, resulting in a decrease in ultimate tensile strength (UTS) for pristine PCL from 3 ± 0.12 MPa to 1.7 ± 0.11 MPa, while the UTS for PCL membranes containing 1.5% nHA increased from 3.3 ± 0.30 MPa to 4.18 ± 0.28 MPa. <i>In vitro</i> bioactivity in simulated body fluid (SBF) showed enhanced apatite formation, particularly after 21 and 28 days. Cytotoxicity assays with MG-63 osteoblast-like cells demonstrated good biological performance. The incorporation of nHA not only improved the mechanical properties but also enhanced the bioactivity and cytocompatibility of the electrospun PCL membranes, making them promising candidates for guided tissue regeneration (GTR) applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1037-1054"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human serum albumin microspheres synchronously loaded with ZIF-8 and triptolide (TP) for the treatment of intrahepatic cholangiocarcinoma.","authors":"Fuxin Li, Ruiqiang Cai, Zipian Ye, Li Yang, Xianshuai Qiu, Xueqiang Sun","doi":"10.1177/08853282251318872","DOIUrl":"10.1177/08853282251318872","url":null,"abstract":"<p><p>Intrahepatic cholangiocarcinoma (ICC) is the second most common primary liver tumor and remains a fatal malignancy in most patients. Only 20% to 30% of patients can be treated with potentially curative surgical resection. Local therapies such as radioembolization and hepatic arterial perfusion may be a more effective treatment strategy. The active ingredients of natural plants have aroused wide interest in the treatment of tumors. Triptolide shows toxic effects on a variety of epithelioid carcinoma cells. However, there is currently a lack of suitable delivery system for the treatment of ICC. In this study, organometallic framework material ZIF-8 was chosen to load TP, and then encapsuled in HSA micro-nanoparticles for the perfusion treatment of ICC. The results of SEM, XRD, and FTIR showed that ZIF-8 nanoparticles were encapsuled in HSA micro-nanoparticles. ZIF-8 nanoparticles (57.89 ± 12.24%) and TP@ZIF-8/HSA (36.8 ± 4.71%) micro-nanoparticles could significantly inhibited proliferation of RBE cell. Also, TP@ZIF-8/HSA micro-nanoparticles of all groups exhibited favorable cytocompatibility to L929 cells and hemocompatibility. RT-qPCR and western blot showed that ZIF-8 and TP induced apoptosis in cancer cells through mitochondria-related pathways. The results demonstrated that TP@ZIF-8/HSA was a potential chemotherapy candidate for the treatment of ICC.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1030-1036"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingival keratinocyte adhesion on atomic layer-deposited hydroxyapatite coated titanium.","authors":"Faleh Abushahba, Sini Riivari, Nagat Areid, Elisa Närvä, Elina Kylmäoja, Mikko Ritala, Juha Tuukkanen, Pekka K Vallittu, Timo O Närhi","doi":"10.1177/08853282251313503","DOIUrl":"10.1177/08853282251313503","url":null,"abstract":"<p><p><b>T</b>his study aimed to evaluate the effects of the atomic layer deposited hydroxyapatite (ALD-HA) coating of the titanium (Ti) surface on human gingival keratinocyte (HGK) cell adhesion, spreading, viability, and hemidesmosome (HD) formation. Grade 2 square-shaped Ti substrates were used (<i>n</i> = 62). Half of the substrates were ALD-HA coated, while the other half were used as non-coated controls (NC). The ALD-HA surface was characterized with scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) analysis. The initial cell adhesion and HD formation of HGKs were evaluated after a 24-h cultivation period. The cell proliferation was assessed by cultivating cells for 1, 3, and 7 d. The expression levels of the integrin mediating cell adhesion were detected with the Western Blot method. In addition, cell spreading and expression of the proteins mediating cell adhesion were imaged using a confocal microscope. SEM-EDS analysis demonstrated the formation of HA on the ALD-HA surfaces. The relative cell attachment was significantly higher (<i>p</i> < .05) on the ALD-HA compared to the NC surface after 1 and 3 d of cell culture. No significant difference was found in integrin α6 or β4 expression. The microscope evaluation showed significantly increased cell spreading with peripheral HD expression on ALD-HA compared to the NC surfaces (<i>p</i> = .0001). Moreover, laminin γ2 expression was significantly higher on the ALD-HA than on the NC surfaces (<i>p</i> < .001). Compared to the NC Ti surface, the ALD-HA coating has favorable effects on HGK proliferation, growth, and cell spreading. This indicates that the ALD-HA coating has good potential for improving mucosal attachment on implant surfaces.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1055-1063"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of polyvinylpyrrolidone-catechol-derived chitosan nanoconjugates allowed for kidney-targeted treatment of cisplatin-induced acute kidney injury and nursing care management.","authors":"Guixian Chen","doi":"10.1177/08853282241304396","DOIUrl":"10.1177/08853282241304396","url":null,"abstract":"<p><p>Acute kidney injury (AKI) resulting from cisplatin (Cs) chemotherapy presents a significant challenge in clinical management. The study aimed to fabricate a novel compound Polyvinylpyrrolidone-catechol-derived chitosan nanoconjugates (PCChi-NC) for targeting Cs-induced AKI. Characterization studies utilizing UV-visible spectrophotometry, FT-IR, XRD, and TEM revealed a spherical morphology with diameters ranging from 20 to 60 nm. In vitro assessments utilizing HEK 293 cell lines demonstrated the biocompatibility of PCChi-NC without eliciting toxic effects. Furthermore, PCChi-NC exhibited a notable reduction in Cs-induced cell death in kidney cells, as evidenced by biomarker analysis. Anti-inflammatory analysis of mouse kidney homogenates revealed a decrease in TNF-α and IL-1β levels, indicative of the therapeutic efficacy of PCChi-NC in mitigating Cs-induced kidney inflammation. Moreover, In vivo, experimental analysis was evidenced by stable body weight and histopathological changes in mice. Our findings highlight the potential of PCChi-NC as a promising candidate for targeted therapy in Cs-induced AKI, owing to its unique renal targeting capacity.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1084-1096"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142947926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional electrospinning periosteum: Development status and prospect.","authors":"Jinli Zhu, Meifeng Li, Shuoshuo Yang, Yang Zou, Yonggang Lv","doi":"10.1177/08853282251315186","DOIUrl":"10.1177/08853282251315186","url":null,"abstract":"<p><p>In the repair of large bone defects, loss of the periosteum can result in diminished osteoinductive activity, nonunion, and incomplete regeneration of the bone structure, ultimately compromising the efficiency of bone regeneration. Therefore, the research and development of tissue-engineered periosteum which can replace the periosteum function has become the focus of current research. The functionalized electrospinning periosteum is expected to mimic the natural periosteum and enhance bone repair processes more effectively. This review explores the construction strategies for functionalized electrospun periosteum from the following perspectives: ⅰ) bioactive factor modification (bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) etc.), ⅱ) inorganic compound modification, ⅲ) drug modification, ⅳ) artificial periosteum in response to physical stimuli. Furthermore, the construction of artificial periosteum through electrospinning, in conjunction with other strategies, is also analyzed. Finally, the current challenges and prospects for the development of electrospinning periosteum are also discussed.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"996-1013"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142965038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium nanoparticles and paclitaxel co-delivery by a PCL based nanofibrous scaffold to enhance melanoma therapy.","authors":"Maryam Doostan, Ata'ollah Rahmani Azar, Hassan Maleki","doi":"10.1177/08853282251330724","DOIUrl":"https://doi.org/10.1177/08853282251330724","url":null,"abstract":"<p><p>The incidence of skin cancer has increased significantly in recent decades, highlighting the need for more effective treatments due to the limitations of traditional approaches. This study focused on creating a poly (ε-caprolactone) and chitosan (PCL/CS) nanofibrous scaffold loaded with selenium nanoparticles (Se NPs) and paclitaxel (PTX) to inhibit melanoma cell growth. The synthesized Se NPs, characterized by their uniform spherical shape and nano-scale size (∼120 nm), were incorporated into the scaffold. Then, the Se NPs and PTX were concurrently loaded into PCL/CS nanofibers at 5 wt%, which resulted in fibers with an average diameter of 253 ± 35 nm, presenting a ribbon-like morphology and absence of droplets/beads. The results indicated a high fluid absorption capacity, a wettability and high tensile strength of the produced scaffold. Moreover, the controlled release of the loaded compounds was provided over several days. Remarkably, high toxicity (>90%) and higher levels of apoptosis (>85%) were observed in A375 melanoma cells treated with the PTX-Se NPs PCL/CS scaffold. Moreover, the assessment of fibroblast growth and hemolysis confirmed the scaffold's high level of biocompatibility. The PTX-Se NPs PCL/CS nanofibers exhibit favorable properties and strong anti-tumor efficacy, making them a promising scaffold for localized and selective chemotherapy in anti-melanoma treatment.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251330724"},"PeriodicalIF":2.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Bone Regeneration with Silybin-Loaded PCL/Gelatin/Nanoclay Nanocomposite Scaffolds: An In Vitro & In Vivo Study.","authors":"Mohammad Hossein Tayeed, Maryam Tehranchi, Arian Ehterami, Fereshteh Shanei, Ferial Taleghani, Hasan Semyari, Saeed Mahdipour Ganji, Nika Mehrnia, Shaghayegh Bozorgzadeh, Sepehr Zamani, Majid Salehi","doi":"10.1177/08853282251329901","DOIUrl":"https://doi.org/10.1177/08853282251329901","url":null,"abstract":"<p><p>This study focuses on the development of a 3-dimensional porous scaffold using Polycaprolactone/Gelatin/Nanoclay (PCL/GNF/NC) for bone tissue engineering. The scaffold incorporates varying dosages of silybin (Sil) through a mixture of electrospinning and thermal-induced phase separation (TIPS) techniques. Assessments of surface shape, porosity, compressive strength, water contact angle, degradation rate, releasing profile, hemolysis, and cell proliferation were among the investigations carried out to appraise the manufactured scaffolds. In vivo evaluation utilized a rat calvaria defect model, with histological analysis employed to assess the results. The scaffolds exhibited porosity within the range of 70-90%, and those containing silybin demonstrated lower compressive strength and contact angle, along with a higher degradation rate compared to those without silybin. Release experiments revealed a 61.09% release of silybin after 28 days. In both in vivo and in vitro assessments, the PCL/GNF/NC/Sil1% scaffold displayed superior cell proliferation and bone healing properties compared to other groups. These findings suggest the potential efficacy of silybin in bone defect treatment, warranting further investigation in future research.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251329901"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silk fibroin-hyaluronic acid nanofibers for peripheral nerve regeneration.","authors":"Madeline R Badrak, Judy Senanayake, Ahmad Zunnu Rain, Harini G Sundararaghavan","doi":"10.1177/08853282251329315","DOIUrl":"https://doi.org/10.1177/08853282251329315","url":null,"abstract":"<p><p>Peripheral nerve injuries are common and a major source of pain that can lead to debilitating loss of function. Current treatments are limited, with autologous nerve grafts being the gold standard treatment for nerve injuries. However, autografting is not always successful and can lead to increased debilitation through donor site morbidity. Tissue engineering seeks to improve nerve injury treatment though the use of nerve conduits. Conduits made from a functional biomaterial can be implanted into a nerve injury site encouraging and controlling axonal regrowth without causing additional harm to the patient. Both silk fibroin (SF) and hyaluronic acid (HA) have been proven successful in the field of neural tissue engineering. SF has excellent mechanical properties and is biocompatible. HA is part of the extracellular matrix and had been used in nerve regeneration applications. This study developed aligned combination SF-HA nanofibers through electrospinning that could be used within a nerve conduit. Both materials were methacrylated to allow for photocrosslinking and additional control over material properties. Methcrylated SF-HA was tested alongside a material containing only methacrylated HA that has already proven to be effective in literature. When characterizing the materials, it was found that through chemical methacrylation HA was substituted at 60% while SF reported a 30% substitution. Electrospun SF-HA nanofibers were found to have a greater diameter than HA fibers; however, SF-HA was found to be more aligned with greater surface hydrophobicity. Mechanically, it was found that both materials exceeded the elastic modulus of native tissue, but SF-HA far exceeded HA in elasticity and overall fiber extension. Furthermore, human Schwann cells attached, proliferated, and released more pro-regenerative growth factors on SF-HA than HA. Dorsal root ganglia neurons also displayed longer neurite extensions on SF-HA fibers. We concluded that SF-HA nanofibers have potential as a nerve conduit material.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251329315"},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of SF-gel-CS-Hap bionic biphasic porous scaffolds and evaluation of physical, mechanical and biological properties.","authors":"Mingxi Gu, Lin Guo, Changcheng Wang, Fengde Tian, Ruihu Hao","doi":"10.1177/08853282251329591","DOIUrl":"https://doi.org/10.1177/08853282251329591","url":null,"abstract":"<p><p><b>Objective:</b> Full-thickness cartilage defect are usually accompanied by subchondral bone damage, which is difficult to self-repair once damaged due to the lack of vascularization and innervation. In this study, a biphasic composite scaffold was developed by combining vacuum freeze-drying and iterative freeze-thawing with gelatin, chitosan, silk fibroin, and hydroxyapatite as the basic materials to explore the feasibility of using them for the repair of total cartilage defects. <b>Methods and Results:</b> Six groups of SF-CS-Gel-nHap porous scaffolds (Hap-0%, Hap-1%, Hap- 2%, Hap-3%, Hap-4%, Hap-5%) were prepared by vacuum freeze-drying and chemical cross-linking using filipin protein (SF), gelatin (Gel), chitosan (CS) and hydroxyapatite (Hap) as the base materials. A series of characterization methods were used to systematically analyze and test the morphological features as well as physical and mechanical properties of the scaffolds. Then a novel bionic biphasic porous scaffold was developed by a combination of freeze-drying and freeze-thawing using the SF-CS-Gel as the cartilage phase and the SF-CS-Gel-2%Hap as the subchondral bone phase. Finally, it was co-cultured with chondrocytes to verify the biological properties of the SF-CS-Gel/SF-CS-Gel-2%Hap bionic biphasic porous composite scaffold in vitro. The results showed that the SF-CS-Gel/SF-CS-Gel-2%Hap biphasic scaffolds had a highly porous mesh structure, with an average pore size of 156.06 ± 42.36 μm in the cartilage phase and 214.38 ± 65.82 μm in the subchondral bone phase. Co-cultured with chondrocytes, the live and dead cells stained, cck-8 growth and proliferation curves showed that the bionic scaffolds had good biocompatibility and cytotoxicity. Cytoskeletal staining showed that the morphology of chondrocytes in the bionic scaffolds could maintain three-dimensional growth as in vivo. <b>Conclusion:</b> The results showed that SF-CS-Gel/SF-CS-Gel-2%Hap biphasic scaffolds have good biocompatibility, biodegradability, stability, appropriate mechanical properties and porosity, and are suitable for repairing articular cartilage and subchondral bone. It is expected to be used as a repair material for articular cartilage in clinical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251329591"},"PeriodicalIF":2.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}