Journal of Biomaterials Applications最新文献

{"title":"Ultraviolet light-functionalized TiO₂ nanotubes for enhancing antibacterial properties and soft tissue integration of titanium implants.","authors":"Wen Han, Yijiao Yuan, Yurong Chen, Wei Wang, Chunhui Lu","doi":"10.1177/08853282261420579","DOIUrl":"10.1177/08853282261420579","url":null,"abstract":"<p><p>Peri-implantitis, a leading cause of dental implant failure, is primarily attributed to suboptimal soft tissue sealing at the implant-tissue interface, which facilitates bacterial colonization and subsequent inflammatory responses. Surface modifications on titanium can expedite good soft tissue sealing and antibacterial action. We engineer titanium surfaces with ultraviolet light-functionalized titanium dioxide nanotubes (UV-TNTs) to achieve good soft tissue sealing and antibacterial effect. Surface properties were characterized via scanning electron microscopy (SEM), surface roughness analysis, wettability assessment and methylene blue (MB) degradation. Antibacterial performance was quantified using <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>) viability assays. Soft tissue integration was evaluated through fibroblast proliferation assays and immunofluorescence staining of vinculin and collagen type I. The UV-TNTs demonstrated significantly enhanced antibacterial efficacy and promoted fibroblast proliferation, adhesion and collagen deposition to improve soft tissue sealing. This surface modification strategy offers a promising approach for enhancing implant biocompatibility and long-term stability, offering useful references for advanced implant design and fabrication.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"447-459"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146179741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gelatin hydrogel cross-linked with glutaraldehyde loaded with methylene blue for photodynamic action in gliosarcoma strain 9 L/lacZ.","authors":"Janicy Arantes Carvalho, Jéssica Aparecida Ribeiro Ambrósio, Pamela Ingrid de Oliveira, Eduardo Leite de Souza, Letícia Gabriela Machado Pinto, Milton Beltrame Junior, Leandro José Raniero, Gustavo Luiz Bueno Cardoso, Ivone Regina de Oliveira, Juliana Guerra Pinto, Luciana Maria Cortez Marcolino, Juliana Ferreira-Strixino, Andreza Ribeiro Simioni","doi":"10.1177/08853282261429830","DOIUrl":"10.1177/08853282261429830","url":null,"abstract":"<p><p>Since the prognosis and treatment of nervous system tumors are still not beneficial to the patient, alternative therapies need to be investigated as primary or supplemental treatments to current methods. Hydrogel systems are well-known for their high-water absorption capacity, three-dimensional network composition, and biocompatibility, making them suitable as photosensitizers (PS) carriers for photodynamic therapy (PDT). A gelatin hydrogel system was synthesized via chemical cross-linking with varying glutaraldehyde concentrations, and the optimal hydrogel was encapsulated with methylene blue (MB). Scanning electron microscopy (SEM) analysis demonstrated that the formulation formed three-dimensional networks. The freeze-drying procedure increases the hydrogel's water-retention capacity, as shown by the swelling test. All spectroscopic results showed excellent photophysical properties of MB when incorporated into the system. The encapsulation efficiency was 95.35%. According to the trypan blue exclusion test, the cell viability in the PDT-treated groups was significantly lower (p < 0.05). Approximately 95% of 9 L/lacZ cells died after PDT utilizing a concentration of 50 μmol.mL^-1 for the hydrogel with MB. Based on the data obtained, the system's viability has been confirmed, and it is expected to demonstrate potential in the treatment of neoplasms.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"387-401"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147326064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of aligned PCL/rGO scaffolds and electrical stimulation on BMSC neuronal differentiation.","authors":"Liqing Xiao, Mengen Li, Na Zhang, Fang Li, Xiaobo Shi","doi":"10.1177/08853282261429738","DOIUrl":"10.1177/08853282261429738","url":null,"abstract":"<p><p>Directed differentiation of bone marrow mesenchymal stem cells (BMSCs) provides a promising route for neural trauma repair, yet faces challenges in establishing an optimal microenvironment for efficient neuronal maturation. In this work, a synergistic bio-electroactive platform was established to specifically induce neuronal differentiation from BMSCs by coupling spatially oriented, conductive polycaprolactone/reduced graphene oxide (PCL/rGO) nanofibrous scaffolds with exogenous electrical stimulation (ES). Through the integration of topological guidance and rGO-enhanced conductivity, significant cytoskeletal remodeling and cell proliferation were realized. Notably, the synergistic stimulation robustly drove the lineage commitment toward mature neurons, evidenced by the distinct neuron-like morphology (spherical-elliptical somas and elongated processes) and the marked upregulation of neural-specific markers (Tuj1 and MAP2) at both transcriptional and translational levels. The PCL/rGO + ES group achieved the highest differentiation efficiency, outperforming PCL/rGO group with an approximate 2.29-fold increase in MAP2 expression, confirming the superior synergy of the dual-signal stimulation. This bio-electroactive strategy effectively creates a conducive microenvironment for mature neuronal differentiation and offers a facile, promising perspective for the treatment of neural trauma.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"460-469"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147270906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable trimagnesium phosphate-filled magnesium potassium phosphate cement with improved handling properties, high early strength, and low heat release for bone defect repair.","authors":"Chang Xiao, Yan Xiang, Shu Wang, Chen Shi, Lei Xie","doi":"10.1177/08853282261422837","DOIUrl":"10.1177/08853282261422837","url":null,"abstract":"<p><p>Magnesium phosphate cement (MPC) has emerged as a promising biomaterial in preclinical studies for orthopedic applications, particularly in bone defect repair, due to its rapid setting properties and high mechanical strength. However, excessive MgO in MPCs accelerates the setting and heat release, which necessitates an increased liquid-to-powder ratio (LPR) that compromises strength, ultimately making it difficult to reconcile the performance metrics of processability, heat release, and mechanical strength. In this study, trimagnesium phosphate-magnesium phosphate cement (TM-MPC) was developed by replacing excess MgO with chemically stable trimagnesium phosphate (Mg₃(PO₄)₂, TMP) at a low LPR (down to 0.20 mL/g, PLR = 5 g/mL). This strategy resulted in the formation of cement material system with suitable setting time (10-12 min), low curing temperature (<47°C), remarkable early compressive strength (131.25 ± 29.39 MPa), near physiological pH, and good injectability (6-9 min), which could address the critical drawbacks of traditional MPCs. <i>In vitro</i> evaluations showed acceptable cytocompatibility in MC3T3-E1 cells. Based on heat evolution and characterization of early hydration products, reducing the LPR was found to retard initial hydration and attenuated exothermic heat release. This work provides a facile and effective strategy for engineering high-performance bone cements with improved processability, which offers a design paradigm for future MPC systems in specific biomedical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"349-362"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146219820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sturgeon collagen fibrils as a promising biomaterial for cartilage tissue engineering.","authors":"Beini Sun, Linyan Shi, Kazuhiro Ura, Yasuaki Takagi, Xi Zhang","doi":"10.1177/08853282261425516","DOIUrl":"10.1177/08853282261425516","url":null,"abstract":"<p><p>Cartilage tissue engineering (CTE) provides a promising solution for osteoarthritis, with scaffold materials playing a crucial role in supporting cell activity. There have been many studies of various scaffold materials, yet few studies have directly compared the effects of marine collagen fibrils on chondrocyte chondrogenesis. This study determined the effects of types I and II collagen fibrils derived from sturgeon on ATDC5 cell chondrogenesis. The CCK-8 results showed that type I fibrils promoted ATDC5 cell proliferation more effectively than type II fibrils. Alcian Blue staining results revealed that cells cultured on type II fibrils secreted more proteoglycans than those on type I fibrils. The mRNA expression analysis indicated that type I fibrils initiated cell differentiation at an early stage, but simultaneously induced premature hypertrophy, suggesting that type I fibrils were difficult to maintain the chondrogenic phenotype in long-term culture. In contrast, type II fibrils generally enhanced the expression of chondrogenic genes, but also upregulated matrix metalloproteinase collagen-degrading enzymes, thereby accelerating the matrix degradation process. These findings suggested the potential of sturgeon collagen as a CTE scaffold material, and identified the different effects of types I and II collagen fibrils on ATDC5 cell chondrogenesis, to provide a theoretical foundation for selecting suitable scaffold biomaterials, based on different needs, while also providing new insights for the application of marine collagen.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"417-430"},"PeriodicalIF":2.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147317090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of starch/polycaprolactone nanofibers loaded with tranexamic acid as a hemostatic agent under in vitro.","authors":"Golnaz Zhand, Fariba Esmaeili, Masoumeh Zahmatkeshan, Vahid Shirshahi","doi":"10.1177/08853282261480965","DOIUrl":"https://doi.org/10.1177/08853282261480965","url":null,"abstract":"<p><p>Uncontrolled hemorrhage remains one of the most critical clinical challenges in trauma, surgery, and minimally invasive dental procedures, highlighting the need for advanced hemostatic biomaterials that integrate rapid coagulation, bioactivity, and structural stability. In this study, electrospun PCL/Starch nanofibrous scaffolds loaded with tranexamic acid (TXA) were developed as a multifunctional hemostatic platform, wherein three complementary components are synergistically integrated at the nanoscale: starch as a bioactive, hydrophilic polymer that enhances blood uptake and promotes platelet adhesion; TXA as a potent antifibrinolytic agent that stabilizes fibrin networks and prevents premature clot degradation; and PCL as a mechanically robust polymer that ensures structural integrity during clinical application. While prior TXA-loaded electrospun systems have predominantly relied on chitosan- or PVA-based matrices, these approaches are associated with batch-to-batch variability, elevated cost, and frequent requirement for chemical crosslinking. In contrast, starch is a naturally abundant, low-cost, and fully biodegradable polysaccharide with an intrinsic hemostatic capacity: its high water absorption and porous structure accelerate blood coagulation by concentrating platelets, red blood cells, and coagulation factors at the injury site, thereby activating the coagulation cascade independently of exogenous drug loading. To the best of our knowledge, based on an extensive review of the available literature, this study represents the first systematic investigation of TXA-loaded PCL/Starch electrospun nanofibers for hemostatic applications, addressing a clear and unmet gap in the field: the absence of a multifunctional scaffold that integrates rapid coagulation induction, fibrinolysis inhibition, and structural stability within a single, scalable, and cost-effective fabrication approach.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261480965"},"PeriodicalIF":2.4,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decellularized fish swim bladder as a dermal graft: Histological, biomechanical, and in vivo characterization.","authors":"Sajedeh Jalali, Roya Lari","doi":"10.1177/08853282261483997","DOIUrl":"https://doi.org/10.1177/08853282261483997","url":null,"abstract":"<p><p>Effective skin substitutes must create a supportive microenvironment that promotes cell attachment, migration, and integration within the scaffold matrix. Decellularized fish swim bladder (DFSB) scaffolds offer a collagen-rich matrix substitute with promising regenerative potential, although species-specific differences in their performance are not yet fully understood. This study aimed to identify the DFSB scaffold that best promotes cellular infiltration and wound healing, with an emphasis on modulating inflammation. In this study, a novel in vivo approach was developed to directly assess cell-scaffold interactions through the integration of cell migration and quantitative infiltration analyses, enabling a physiologically relevant evaluation beyond conventional in vitro methods. To achieve this DFSBs from four fish species were evaluated for mechanical properties, swelling behavior, and porosity. The scaffold with the most favorable structural characteristics was implanted into full-thickness wounds. Wound-healing parameters and quantitative cell infiltration into the scaffold were assessed on days 1, 3, 5, and 7. The results indicated that Rutilus frisii kutum demonstrated the most suitable properties, including preserved collagen architecture, moderate swelling and porosity. Upon implantation, the scaffold adhered firmly to the wound bed and remained stable throughout healing, enabling direct and continuous scaffold-wound interaction. Cells attached within 24 h and significantly infiltrated deeper into the matrix over time, with marked increases in cell density and penetration depth by day 5 (<i>p</i> < 0.001). This effective interface corresponded with accelerated wound repair, including earlier epithelialization (day 1), reduced inflammation, and enhanced fibroblast recruitment, angiogenesis, and collagen deposition. In conclusion the Rutilus frisii kutum DFSB scaffold provided a supportive matrix for rapid cell attachment and deep infiltration. Its stable integration with the wound bed enabled continuous cell-scaffold interaction, contributing to inflammation modulation and accelerated healing.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261483997"},"PeriodicalIF":2.4,"publicationDate":"2026-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired antioxidative HA-CPA hydrogels for restoring cellular and vascular function in diabetic wound repair.","authors":"Yangxi Chen, Ying Chen, Li Huang, Qian Zhang","doi":"10.1177/08853282261477512","DOIUrl":"https://doi.org/10.1177/08853282261477512","url":null,"abstract":"<p><p>Chronic diabetic wounds are difficult to treat because persistent oxidative stress, mitochondrial dysfunction, and impaired stromal-vascular communication jointly delay tissue repair. In this study, we developed a bioinspired hyaluronic acid-catechol-PEG-amine (HA-CPA) hydrogel through dynamic Schiff-base crosslinking for topical diabetic wound management. The HA-CPA hydrogel formed a porous, self-supporting network and showed favorable cytocompatibility and hemocompatibility. Owing to catechol-containing moieties, the hydrogel displayed strong antioxidant activity, with more than 80% radical-scavenging efficiency in DPPH and ABTS assays. Under diabetic-mimicking conditions, HA-CPA hydrogel extracts reduced intracellular reactive oxygen species, helped preserve mitochondrial membrane potential, and improved fibroblast migration and endothelial angiogenic behavior, including tube formation. In a streptozotocin-induced diabetic full-thickness wound model, HA-CPA treatment accelerated wound closure, enhanced collagen deposition, and promoted re-epithelialization without detectable systemic toxicity in major organs. These findings indicate that HA-CPA hydrogel provides a practical wound-dressing platform that combines redox regulation, inflammatory microenvironment modulation, cellular protection, and vascular-supportive activity.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261477512"},"PeriodicalIF":2.4,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148765095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-camouflaged liposomes Co-delivering L-Arg and NMN for reprogramming macrophage nitric oxide metabolism in atherosclerosis.","authors":"Xuting Li, Yi Yuan, Changhong Lian, Dongdong Zhu, Yan Wang, Zhenlei Jia","doi":"10.1177/08853282261476791","DOIUrl":"https://doi.org/10.1177/08853282261476791","url":null,"abstract":"<p><p>Atherosclerosis progression is closely linked to metabolic dysfunction of plaque macrophages, where inducible nitric oxide synthase (iNOS)-mediated pathological nitric oxide (NO) burst is a key driver of inflammation and oxidative stress. This study designed macrophage membrane-camouflaged liposomes (M-AN@Lip) for the co-delivery of nicotinamide mononucleotide (NMN) and L-arginine (L-Arg), aiming to precisely regulate macrophage NO metabolic homeostasis. In vitro experiments demonstrated that M-AN@Lip effectively increased intracellular NO generation while significantly downregulating the expression of the oxidative stress-related enzyme NAD(P)H quinone oxidoreductase 1 (NQO1), suggesting a reprogramming of macrophage NO metabolism from a pathological iNOS-dominated pathway towards a protective one. This metabolic shift was accompanied by clearance of intracellular reactive oxygen species (ROS), enhancement of the endogenous antioxidant enzyme system, and polarization of macrophages towards an anti-inflammatory M2 phenotype. Ultimately, M-AN@Lip significantly inhibited oxidized low-density lipoprotein (ox-LDL) uptake and foam cell formation by downregulating the expression of scavenger receptors (CD36, MSR1, SRB1). This study demonstrates that a biomimetic nano-system can achieve integrated anti-inflammatory, antioxidant, and anti-foam cell forming effects through the synergistic regulation of NO metabolism, offering a promising novel nanotherapeutic strategy for atherosclerosis.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261476791"},"PeriodicalIF":2.4,"publicationDate":"2026-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148712577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An injectable GelMA hydrogel improves Achilles tendon repair through local microenvironment modulation.","authors":"Mingyu Yang, Li Hu, Rui Chao, Shengli Zhang, Xiaoxing Zhang, Tianjun Cheng, Lei Peng, Ruoyu Song, Mingrui Lyu, Jian Liu, Zhidong Cao","doi":"10.1177/08853282261474963","DOIUrl":"https://doi.org/10.1177/08853282261474963","url":null,"abstract":"<p><p>Achilles tendon injury remains a major clinical challenge because of its limited intrinsic healing capacity and the high risk of disorganized extracellular matrix remodeling during repair. In this study, we developed an injectable gelatin methacryloyl (GelMA) hydrogel loaded with GsMTx4, a mechanosensitive ion channel modulator, for sustained local delivery and evaluated its therapeutic potential for Achilles tendon healing in vitro and in vivo. The hydrogel exhibited favorable physicochemical properties, injectability, and local retention capacity. Biological evaluation showed that treatment with this delivery system was associated with improved cell compatibility, reduced matrix degradation, and enhanced expression of tendon-related markers. In the Achilles tendon injury model, local administration promoted histological repair, improved collagen organization, and alleviated pathological changes in the injured tissue. These beneficial effects were accompanied by modulation of extracellular matrix remodeling and mechanosensitive responses. Collectively, these findings suggest that this injectable GelMA hydrogel-based delivery strategy may serve as a promising local therapeutic approach for improving Achilles tendon repair.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261474963"},"PeriodicalIF":2.4,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148684583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}