Journal of Biomaterials Applications最新文献

{"title":"Injectable ADM temperature-sensitive hydrogel loaded with bFGF in diabetic rat wound healing study.","authors":"Haojiao Chen, Jianping Sun, Wenyang Liu","doi":"10.1177/08853282251321943","DOIUrl":"10.1177/08853282251321943","url":null,"abstract":"<p><p><b>Background:</b> Diabetic wound is one of the most common diabetic chronic complications. Effective treatments of diabetic wound remain limited. Here, we explored the effects of basic fibroblast growth factor (bFGF)-acellular dermal matrix (ADM) hydrogel on the diabetic wound. <b>Methods:</b> The bFGF-ADM hydrogel was manufactured by mixing 180 µL ADM hydrogel and 20 µL bFGF aqueous solution (10 mg/mL). The morphology of ADM hydrogel and bFGF-ADM hydrogel was observed under scanning electron microscope. The release property of bFGF-ADM hydrogel was determined by ELISA. CCK-8 assay was utilized to estimate the cell viability of mouse skin fibroblasts. The diabetes mellitus (DM) model was established in rats. The four wounds on the back of each DM rat were treated with the ADM hydrogel, bFGF-ADM hydrogel, bFGF aqueous solution and no solution (control), respectively. The wound healing rate of each rat was estimated. The traumatized skin tissue of each rat was observed by H&E staining and Sirius red staining. <b>Results:</b> The bFGF-ADM hydrogel displayed an interconnected pore structure and bFGF was gradually released from the bFGF-ADM hydrogel over time. The bFGF-ADM hydrogel could enhance the cell viability of skin fibroblasts and promote the wound healing rate, the re-epithelialization of wound and increase the collagen fiber content of dermis. And the bFGF-ADM hydrogel exhibited better therapeutic effects of diabetic wound than either bFGF or ADM alone. <b>Conclusions:</b> Our study revealed that the bFGF-ADM hydrogel could promote diabetic wound healing.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1156-1164"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexamethasone loaded blend poly hydroxybutyrate-stearic acid nanoparticles for ocular inflammation.","authors":"Ebru Erdal, Nagihan Uğurlu, Esin Akbay Çetin, Nelisa Laçin Türkoğlu, Cafer Çakal, Murat Demirbilek","doi":"10.1177/08853282251324619","DOIUrl":"10.1177/08853282251324619","url":null,"abstract":"<p><p>The aim of this study is to develop and analyze dexamethasone-loaded poly hydroxybutyrate-stearic acid blend nanoparticles for the treatment of non-bacterial uveitis. Uveitis is a chronic inflammatory eye disease responsible for 10-15% of global blindness. While repeated intravitreal steroid injection is a successful treatment strategy, it has drawbacks such as cataracts and retinal detachment. Serious side effects and high costs caused by recurrent infections are important limiting factors. Controlled release systems are preferred to maintain therapeutic drug concentrations in the vitreous humor while minimizing the frequency of injections. In this study, PHB-stearic acid blend nanoparticles with varying ratios of dexamethasone suitable for intravitreal injection were fabricated. It was assessed the effects of the stearic acid ratio on loading efficiency, release profiles, size distribution, and thermal properties. The results indicated that an increase in the stearic acid ratio led to a reduction in nanoparticle diameter and an enhancement in the dexamethasone loading capacity. Additionally, higher stearic acid ratios resulted in a decrease in the melting enthalpy of the nanoparticles, which expedited dexamethasone release. Importantly, the nanoparticle formulations exhibited no toxicity to eye endothelial cells or THP-1 cells.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1211-1221"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of dual drug loaded magnetic gold nanohybrid system for osteoarthritis.","authors":"Gul Rehman, Jadoon Khan, May M Alrashed, Muhammad Abbas, Masood Ur Rehman, Tawaf Ali Shah, Kotb A Attia, Arif Ahmed Mohammed","doi":"10.1177/08853282251324352","DOIUrl":"10.1177/08853282251324352","url":null,"abstract":"<p><p>Cartilage deterioration in patients with osteoarthritis presents a significant challenge, primarily attributable to the inadequate oral bioavailability and poor dosage compliance of chondroprotective agents. The Chondroitin Sulphate (CS) is a stabilizing and reducing agent for metal NP as well as homing agent by binding to surface molecules (CD44, L-selectin, P-selectin, and annexin-6) of chondrocytes at the OA site. This study was designed to develop intra-articular magnetic gold nanohybrids for the co-delivery of chondroitin sulfate, glucosamine sulfate, and gold, aiming to achieve synergistic anti-inflammatory and cartilage regenerative effects and in vitro assessments of drug release were conducted. Additionally, in animal study, the male albino rats underwent anesthesia by inhaling isoflurane using the open-drop exposure method, and chondrocytes were then harvested for cytotoxicity and biocompatibility assays. Physical characterization revealed 66 nm particle size with uniform distribution and colloidal stability of MGN-CS-GS. Zeta potential and FTIR analysis showed electrostatic interaction between the carboxyl and amino groups of MGN-CS and GS. VSM and EDX confirmed paramagnetic and core-shell characteristics of nanohybrids, respectively. It was found that the MGN-CS-GS released more CS (72%) and GS (85%) at acidic pH with continuous release pattern, which will improve patient compliance. The nanohybrid's cytotoxicity assay demonstrated excellent biocompatibility and cellular viability of OA chondrocytes triggered by interleukin-1β (IL-1β) compared to marketed formulation. The results demonstrated that MGN-CS-GS continuously released both drugs with high biocompatibility and cellular viability of OA chondrocytes. The successful synthesis of MGN-CS-GS is a foundation for further research on its potential application as a novel co-drug carrier nanohybrid system.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1222-1239"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation of macrophage cell lines confined with silica and/or silicon particles and embedded in structured collagen gels.","authors":"Pedro U Muñoz-González, Jorge Delgado, Gerardo González-García, Birzabith Mendoza-Novelo","doi":"10.1177/08853282251319875","DOIUrl":"10.1177/08853282251319875","url":null,"abstract":"<p><p>Macrophages encapsulated in composite gels are subjected to a three-dimensional (3D) microenvironment and material-related stimuli that allow modulation of their phenotypes. Herein, 3D collagen fibrillar networks structured with di- or tri-functionalized oligourethanes, including Si-O or Si-Si particles confined therein, are compared regarding their physicochemical properties and material-guided macrophage activation. Gelation kinetics, degradation/swelling, and rheometric results demonstrated that the properties of the composite gels depend on the oligourethane functionalization number (derived from diols/triols and L-Lysine diisocyanate, LDI) and silica incorporation. Human or murine macrophages seeded or encapsulated in the composite gels showed good viability and the adoption of an anti-inflammatory phenotype in response to the silica in the composite gel, showing accelerated gelation when cell culture components are present in the liquid precursors. An increase in cell viability proportional to the storage modulus was observed. ELISA tests strongly suggest that the Si-Si nanoparticles in the composites can antagonize the pro-inflammatory stimulation with lipopolysaccharides (LPS) and interferon-gamma (IFNγ), even promoting an anti-inflammatory response in embedded cells after 24 h. Silicon-doped and crosslinked collagen gels have good potential to modulate macrophage inflammatory response, serving as a 3D immunomodulatory scaffold.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1240-1257"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalin loaded monodispersed polycaprolactone (PCL) microspheres preparation by polyvinylidene fluoride (PVDF) membrane emulsification for wound healing in dermal fibroblasts.","authors":"Wenwei Zhong, Xiang Cao, Haoran Zeng, Manqun Tang, Guizhen Li, Jia Xiong, Yunong Wang, Liwei Guo, Jing Ji","doi":"10.1177/08853282251321259","DOIUrl":"10.1177/08853282251321259","url":null,"abstract":"<p><p>The development of injectable bio-stimulating polycaprolactone (PCL) microspheres for wound healing has strict requirement on size and morphology control, particularly favoring microspheres within the range between 20-50 µm. PCL microspheres with smaller sizes are phagocyted at rapid rate while larger microspheres could cause inflammation. Homogenization can be regarded as an irreversible process to generate microspheres of particular size range while it still remains as the most common approach for microspheres production. Membrane emulsification technology shows great potential in fine tailoring of microspheres while still holds promising ability for scale-up production. Membranes with uniform large pores and dual hydrophilicity might be capable of the production of large microspheres via emulsification with tailorable size distribution. The aim of this study is to verify the feasibility of PVDF membranes with large pores on the generation of PCL microspheres via the combined crystallization diffusion (CCD) approach. The effect of dope solution concentration and PVDF molecular weights on membrane morphologies and the corresponding microspheres characteristics were investigated. Results showed that concentration of 20 wt% produced microspheres at desirable size of 24.14 µm and the optimal span of 0.53. Microspheres with narrow distribution showed the highest drug loading efficiency of baicalin at 8.42 %. The baicalin loaded PCL microspheres presented gradual release of drug release over 33-day of in vitro testing and significantly improved cell growth rate of 111.67 % as compared to the ones prepared by homogenization approach. The wound healing ability was enhanced after the treatment of baicalin-loaded PCL microspheres as compared to empty loaded PCL microspheres.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1143-1155"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physical characterization of PEGylated exosome constructs: Size, charge, and morphology changes in non-specific alkylating N-terminal reactions.","authors":"Andrés Martínez-Santillán, José González-Valdez","doi":"10.1177/08853282251323198","DOIUrl":"10.1177/08853282251323198","url":null,"abstract":"<p><p>Small extracellular vesicles, commonly referred to as exosomes, withhold a promising future in the pharmaceutical industry as carriers for targeted drug delivery due to their high specificity and bioavailability when compared to synthetic-based vectors. They, however, present some limitations for systematic administration because of natural organism defenses and their high-water solubility, ultimately making it difficult for them to reach the intended target. To improve the delivery capacity of these nanoparticles, the possibility for the construction of PEGylated versions was explored in this work. This process was performed, analyzed, and characterized using N-terminal specific PEGylation reactions targeted to the protein contents in the exosomal membrane. For this, two different mono-methoxy polyethylene glycols (mPEG) of 5 and 20 kDa were reacted with exosomes under alkylating conditions. The resulting 5k and 20k PEGylated exosome constructs were characterized and compared with unmodified exosomes, using size, morphology, and zeta potential as comparison parameters. Results after analysis showed an absorbance reduction of approximately 65% and 34% (for the 5 and 20 kDa conjugates respectively), a reduction of 10 to 20% in peak resolution, particle size increase corresponding to the polymer sizes used, and a slight reduction in electric distribution of about 2 to 3 mV less than the unmodified vesicles. The data obtained may provide insights for the optimization of exosome PEGylation strategies for therapeutic use.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1202-1210"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thioredoxin-loaded nanocomposite wound dressing for the delivery of adipose derived stem cells for wound healing applications.","authors":"Jingyi Yang, Zheng Zhang, Lin Jing, Junwen Ge, Dan Deng","doi":"10.1177/08853282251336554","DOIUrl":"https://doi.org/10.1177/08853282251336554","url":null,"abstract":"<p><p>In the current research, Thioredoxin was loaded into chitosan nanoparticles and then loaded into the matrix of collagen hydrogel containing adipose-derived stem cells (ASCs). In vitro studies including Scanning electron microscopy imaging, cell viability assay, cell migration assay, swelling assay, release assay, radical scavenging assay were performed in order to characterize the dressings. Then, the wound healing activity of these scaffolds were studied in a rat model of wound healing. Our findings indicate that the scaffolds markedly accelerated wound closure, enhanced epithelial regeneration, and increased collagen deposition. The wound closure values for the developed dressings were 60.507 ± 2.287% on Day 7 and 95.270 ± 2.600% on Day 14. ELISA results demonstrated an upregulation of VEGF, b-FGF, and TGF-β expression, while TNF-α and IL-6 levels were significantly reduced. For our developed dressings, VEGF levels were 661.307 ± 80.195 pg/mL, while bFGF was detected at 524.410 ± 81.040 pg/mL. The concentration of TGF-β was 315.357 ± 54.783 pg/mL, and TNF-α was measured at 176.093 ± 43.934 pg/mL. Additionally, IL-6 levels were found to be 187.577 ± 40.860 pg/mL. Our results suggest that our developed hydrogel system has improved wound healing via improving angiogenesis and modulating inflammation. These mechanisms can be attributed to the proangiogenic and immunomodulatory activities of ASCs and the antioxidative properties of Thioredoxin.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251336554"},"PeriodicalIF":2.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> characterization of 3D printed polycaprolactone/graphene oxide scaffolds impregnated with alginate and gelatin hydrogels for bone tissue engineering.","authors":"Shaghayegh Amini-Mosleh-Abadi, Zahra Yazdanpanah, Farinaz Ketabat, Mahya Saadatifar, Mohammad Mohammadi, Nima Salimi, Azade Asef Nejhad, Ali Sadeghianmaryan","doi":"10.1177/08853282251336552","DOIUrl":"https://doi.org/10.1177/08853282251336552","url":null,"abstract":"<p><p>To achieve successful bone tissue engineering (BTE), it is necessary to fabricate a biomedical scaffold with appropriate structure as well as favorable composition. Despite a broad range of studies, this remains a challenge, highlighting the need for a better understanding of how structural features (e.g., pore size) and scaffold composition influence mechanical and physical properties, as well as cellular behavior. Therefore, the objective of this study was to characterize physical properties (swelling, degradation), mechanical properties (compressive modulus), and cellular behavior in relation to varying compositions (referred to composite and hybrid scaffolds) as well as varying pore sizes in three-dimensional (3D) printed scaffolds. Composite scaffolds were fabricated from polycaprolactone (PCL) and two different graphene oxide (GO) (3% and 9% (w/v)) concentrations. Additionally, hybrid scaffolds were fabricated by impregnating 3D printed scaffolds in a hydrogel blend of alginate/gelatin. Pore sizes of 400, 1000, and 1500 μm were investigated in this study to assess their effect on the scaffold properties. Our findings showed that swelling and degradation properties were enhanced by (I) the addition of GO as well as introduction of both hydrogel and highest concentration of GO (9% (w/v) GO) into the polymeric matrix of PCL, and (II) increasing the pore size within the scaffolds. Mechanical testing revealed that compressive elastic modulus increased with decreasing pore size, incorporation of GO, and increasing GO content into the matrix of PCL. Although our investigated scaffolds with various pore sizes did not show comparable elastic moduli to that of cortical bone, they exhibited an elastic modulus range (∼31-48 MPa) matching that of trabecular bone. The highest compressive modulus (∼48 MPa) was observed in scaffolds of PCL/9% (w/v) GO (composite scaffolds) with the pore size of 400 μm. Cell viability assay demonstrated high MG-63 cell survival (greater than 70%) in all composite and hybrid scaffolds (indicating scaffold biocompatibility) except PCL/3% (w/v) GO scaffolds. The findings of this study contribute to the field of BTE by providing scaffold design insights in terms of pore size and composition.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251336552"},"PeriodicalIF":2.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-adherence capacity of phytosphingosine on titanium surfaces.","authors":"Enni Liinoja, Nagat Areid, Elisa Närvä, Floris J Bikker, Vuokko Loimaranta, Timo O Närhi","doi":"10.1177/08853282251334902","DOIUrl":"10.1177/08853282251334902","url":null,"abstract":"<p><p>Firm soft tissue attachment on oral implant components together with good bacterial control are important prerequisites for uneventful implant healing. TiO₂ coatings have been shown to enhance human gingival fibroblast attachment, but the coating does not have antimicrobial properties. Phytosphingosine (PHS) is known to have antifouling properties against the cariogenic bacterium <i>Streptococcus mutans (S. mutans)</i> which is also among the first colonizers on implant surfaces. This makes PHS an interesting agent to prevent microbial adhesion on dental implant surfaces. The aim of this study was to examine the impact of PHS on <i>S. mutans</i> and human gingival fibroblast adhesion on titanium surfaces with or without TiO₂ -coating. Titanium discs (<i>n</i> = 99, diameter 14 mm, thickness 1 mm) were fabricated for the study. The discs were divided into four groups: (1) non-coated discs (NC), (2) titanium discs with hydrothermally induced TiO₂ coatings (HT), (3) NC discs treated with PHS solution and (4) HT discs treated with PHS solution. Hydrophilicity of the discs was evaluated by water contact angle measurement. <i>S. mutans</i> was added on HT and NC discs with or without PHS treatment for 30 minutes and the number of attached bacteria was estimated by plate counting method. For fibroblast experiment, the cells were plated on the discs and the number of adhered fibroblasts was determined at three time points (1, 3, 6 h). Additionally, confocal microscope images were obtained to examine fibroblast and <i>S.</i> <i>mutans</i> adhesion and to evaluate cell spreading. PHS treatment significantly decreased the hydrophilicity of HT and NC titanium surfaces (<i>p</i> < .001). <i>S. mutans</i> adhesion was significantly reduced after PHS treatment on both NC (<i>p</i> < .001) and HT surfaces (<i>p</i> < .001). Fibroblast adhesion was significantly reduced in HT group at 1 and 3h time points (<i>p</i> < .001), situation leveling out by the 6th hour. PHS reduced the number of adhered fibroblasts to the surface at incubation times of 1 hours (<i>p</i> = .0011) and 3 hours (<i>p</i> = .0194). At the 6 hour time point the number of adhered cells was no longer reduced, but still a reduction in cell spreading on the surface was observed (<i>p</i> < .05). The adhesion differences were present only in HT group. The PHS treatment reduced adherence <i>of S. mutans</i> and fibroblasts on TiO₂ coated titanium, which may result from reduced hydrophilicity of the surfaces. The dual approach of PHS treatment and TiO₂ coating could provide microbial antifouling properties of dental implants but may also affect fibroblast adhesion.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251334902"},"PeriodicalIF":2.3,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12267862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theranostic performance of EGFR-targeted ceria-based nanoparticles on EGFR-positive cancers.","authors":"Kochurani K Johnson, Pramod Koshy, Chantal Kopecky, Michelle Devadason, Jeff Holst, Kristopher A Kilian, Charles C Sorrell","doi":"10.1177/08853282251336556","DOIUrl":"https://doi.org/10.1177/08853282251336556","url":null,"abstract":"<p><p>EGFR is overexpressed in several cancers and hence EGFR-targeted theranostics is a promising approach to manage cancers, with widespread applicability. When nanoceria, which possesses intrinsic anticancer properties, is conjugated with EGFR-targeted fluorophore-tagged ligands, this nanoformulation can both image tumors and kill them through ROS-mediated cell destruction. Further, targeting enhances the cellular uptake of nanoparticles through EGFR-mediated endocytosis. The present work evaluates the <i>in vitro</i> theranostic performance of FITC-tagged EGF-functionalized nanoceria on EGFR-positive cancers. Three EGFR-positive cell lines were used for the study: MDA-MB-231, PANC-1 and HeLa. The EGFR-binding specificity of the EGF-functionalized nanoparticles was confirmed using western blot analysis. The therapeutic and diagnostic activities of the theranostic nanoparticles were confirmed, the former by cell viability assays and ROS measurements and the latter by confocal imaging. The results demonstrate significant ROS elevation levels for the treated cells and hence the suitability of the particles for therapeutic applications. The nanoparticles also are capable of detection using fluorescence imaging following 5 minutes of treatment, thus confirming the applicability for imaging. Hemolysis assay studies revealed excellent hemocompatibility of the nanoparticles, confirming their suitability for <i>in vivo</i> applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251336556"},"PeriodicalIF":2.3,"publicationDate":"2025-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}