Journal of Biomaterials Applications最新文献

{"title":"Quercetin-loaded MgO nanoparticles in a chitosan/gelatin/PVA matrix enhance KGF1 expression and accelerate wound healing.","authors":"Najmeh Darvishi, Somayeh Reiisi, Sadegh Shirian","doi":"10.1177/08853282251399589","DOIUrl":"10.1177/08853282251399589","url":null,"abstract":"<p><p>Effective treatment of skin wounds is essential due to the skin's protective, regulatory, and aesthetic functions. Post-injury infections can significantly impair healing, highlighting the need for advanced biomaterials that combine antimicrobial activity with regenerative potential. In this study, we developed a multifunctional chitosan/gelatin/polyvinyl alcohol (CS/GEL/PVA) nanocomposite containing magnesium oxide (MgO) nanoparticles loaded with quercetin (MgO@QC), aimed at enhancing wound healing and promoting keratinocyte growth factor 1 (KGF1) expression. MgO nanoparticles were synthesized and characterized using DLS, zeta potential, FTIR, XRD, FESEM, and TEM. Quercetin was successfully loaded onto the MgO nanoparticles with a high loading efficiency of 99%, as confirmed by spectroscopic analyses. The resulting nanocomposite demonstrated favorable physicochemical properties, including uniform morphology, excellent swelling behavior (∼79%), optical clarity, and robust structural integrity. Hemolysis assays revealed excellent hemocompatibility, while in vitro cytotoxicity tests confirmed biocompatibility up to 500 µg/mL. Cell proliferation and migration assays (MTT and scratch test) showed dose-dependent enhancement of fibroblast activity, particularly at 1 mg/mL. The nanocomposite also significantly upregulated KGF1 gene expression, suggesting its role in stimulating epithelial regeneration. In vivo studies using a murine excisional wound model demonstrated accelerated wound closure and tissue regeneration in the MgO@QC-treated group, supported by histological evidence of angiogenesis, re-epithelialization, and reduced inflammation. The CS/GEL/PVA/MgO@QC nanocomposite offers a biocompatible and bioactive platform that significantly enhances wound healing. These findings suggest its strong potential for clinical application as an advanced wound dressing for acute and chronic skin injuries.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1332-1348"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145540563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verification of renal tissue regeneration using human perinephric fat-derived collagen sheet in a partially nephrectomized animal model.","authors":"Bum Soo Kim, Eun Hye Lee, Bo Hyun Yoon, Minji Jeon, Jun-Koo Kang, Jae-Wook Chung, Phil Hyun Song, Tae Gyun Kwon, Yun-Sok Ha","doi":"10.1177/08853282251399548","DOIUrl":"10.1177/08853282251399548","url":null,"abstract":"<p><p>Chronic kidney disease (CKD), a global health issue, affects approximately 10% of the population. However, limited treatment options, such as dialysis or transplantation, have significant drawbacks. Therefore, this study aims to investigate the potential of a collagen sheet derived from human perirenal adipose tissue for kidney regeneration. Collagen sheets were derived from discarded perirenal adipose tissues and implanted into partially nephrectomized mice. The right kidneys were completely removed, and 2 mm of the upper and lower poles of the left kidneys were resected. A collagen sheet measuring 1 × 1 × 3 mm³ was implanted in the mid-pole of the left kidney following partial resection of renal parenchyme. Renal function, inflammation, and tissue regeneration were evaluated using serum analysis, PCR, histological staining, and immunohistochemistry to assess structural and functional improvements. The collagen sheet reduced pro-inflammatory markers, minimized fibrosis, and restored renal function indicators such as BUN and cystatin C, though creatinine levels remained unchanged. Regenerative markers, including PAX2 and Wt1, were significantly elevated, indicating enhanced tissue repair and structural recovery. The perirenal adipose tissue-derived collagen sheet demonstrated anti-inflammatory effects and promoted renal tissue regeneration. These findings suggest its potential as a biomaterial for renal injury management. However, further research is needed to evaluate long-term efficacy, optimize application methods, and ensure clinical safety.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1377-1387"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145540784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological and biochemical characterization of novel dual-layer amnion and full-thickness perinatal tissue wound care allografts.","authors":"Emily S DiNicola, Jerry Chang, Jeremy J Mercuri","doi":"10.1177/08853282251398626","DOIUrl":"10.1177/08853282251398626","url":null,"abstract":"<p><p><b>Aims:</b> Processed perinatal tissue allografts have emerged as adjunctive treatment options for chronic wounds. Different processing techniques used to manufacture perinatal tissue allografts can substantially alter their material and biochemical properties. Thus, the aim of this study was to perform multi-scale characterizations of a dual-layer amnion and full-thickness amnion/chorion allograft. <b>Methods:</b> Histological and biochemical techniques were used to evaluate the extracellular matrix (ECM) microarchitecture and composition of a dual-layer amnion and a full-thickness amnion/chorion allograft. Established assays were performed to quantify graft sulfated glycosaminoglycan (sGAG), collagen, growth factor, and cytokine content. <i>In vitro</i> cellular responses, including proliferation, metabolic activity, and migration of human dermal fibroblasts (HDFs) was used to assess bioactivity of graft extracts. <b>Results:</b> Histological analysis of dual-layer amnion and full-thickness amnion/chorion grafts demonstrated preservation of native ECM layers containing intact cell nuclei, GAGs, collagen, and elastin. sGAG and collagen content of the grafts were comparable to native tissue values reported in literature. Angiogenic, regenerative, matrix remodeling, immunomodulatory, and neurotrophic growth factors were found in dual-layer amnion and full-thickness amnion/chorion grafts. Both grafts induced a significant increase in metabolic activity of HDFs compared to negative controls. <b>Conclusion:</b> Dual-layer amnion and full-thickness amnion/chorion wound care allografts are comprised of an intact microarchitecture containing a variety of ECM components that can provide bioactive signals to HDFs.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1362-1376"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of salt leaching process parameters on the morphology and properties of 3D porous PLA and PEG/PLA scaffolds.","authors":"Ioanna-Georgia Athanasoulia, Petroula A Tarantili","doi":"10.1177/08853282251397732","DOIUrl":"10.1177/08853282251397732","url":null,"abstract":"<p><p>In this study, porous PLA structures were prepared using the porogen leaching technique, specifically with sodium chloride (NaCl) of particle sizes 200-300 µm and 400-500 µm, and polyethylene glycol (PEG) with molecular weights of 3,000, 6,000, or 10,000 g/mol. Scanning electron microscopy (SEM) characterization of the cross-sections revealed that larger NaCl particle sizes contributed to an increased degree of pore connectivity, while PEG with the lowest molecular weight accelerated the leaching process. As the concentration of NaCl in the polymeric matrix increased, its removal became more effective, as indicated by lower residual percentages during thermogravimetric analysis (TGA). Additionally, lower residual percentages were recorded for the systems containing PEG prior to leaching. Although the average diameter of the resulting pores decreased in systems that used PEG, the porous structure achieved was more uniform, with both micro- and macro-porosity observed on the surfaces of the scaffold cross-sections. This variation in pore geometry is desirable and can be tailored for specific applications in scaffold construction for tissue engineering. Water exposure altered the inherent properties of PLA, affecting its suitability for short-term, soft-tissue compatible scaffolds. Solution viscometry revealed a molecular weight drop to contribute to accelerated biodegradation. Differential Scanning Calorimetry (DSC) showed a decrease in the glass transition temperature (T_g), and in cold crystallization temperature (T_cc). In addition, the thermal degradation resistance of PLA decreased, as determined by TGA experiments. The aforementioned changes were significantly amplified in PLA specimens subjected to dual leaching of both PEG and NaCl.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1286-1308"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed topologically adjustable oxygen-supply scaffolds for angiogenesis and bone regeneration.","authors":"Wei Liu, Yuyu Zhang, Zhibin Qiu, Zekun Zhang, Hong Hu, Zheng Xie, Mei Tu, Tao Huang","doi":"10.1177/08853282251395195","DOIUrl":"10.1177/08853282251395195","url":null,"abstract":"<p><p>Degradation of Silk fibroin (SF) provides essential nutrients such as amino acids and peptides for cell proliferation, but cannot provide a slow and sustained O₂ release for osteoblastogenesis, which limits the bone repair effects. For the fabrication of highly personalized and complex bone repair scaffolds, 3D printing technology acts as a tailored tool for the clinical challenge. Therefore, we designed a SilMA/XLG/CaO₂ scaffold system for O₂ supply, which consists of modified photo-crosslinking SF (SilMA), lithium magnesium silicate (XLG) and CaO₂. The combination of modified SF (SilMA) and lithium magnesium silicate (XLG) improves the printability and topological controllability, promoting vascularization and osteogenesis differentiation. Besides, the multi-dimensional modification of CaO₂ enhances the mechanical properties of the scaffolds as well as the adjustability of the O₂ release, providing favorable conditions for osteoblastogenesis. Most importantly, the topology and oxygen release of the 3D printed scaffolds synergistically induced neovascularization and osteoblast differentiation with Mg²⁺ generated by scaffold degradation. Mechanistically, SilMA/XLG/CaO₂ upregulates of angiogenic factors VEGF, CD31, and key osteogenesis proteins RUNX2 and BMP-2, resulting in collagen production and calcium deposition. Overall, our study provides a new strategy for bioactive scaffold preparation that exhibits significant clinical potentials for complex bone defects.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1274-1285"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145452045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-functionalized nanomaterials for controlled drug delivery and regenerative therapies in retinal diseases.","authors":"Razieh Dashti, Fariba Safaei, Golfam Sadeghian, Seyyed Abed Hosseini, Milad Salimibani","doi":"10.1177/08853282251395196","DOIUrl":"10.1177/08853282251395196","url":null,"abstract":"<p><p>Degenerative retinal diseases, such as diabetic retinopathy, age-related macular degeneration (AMD), and retinitis pigmentosa, cause irreversible vision loss by destroying vital retinal cells and represent major global health concerns. Traditional therapies have limited success in fully restoring vision due to the complex retinal structure and blood-retinal barriers (BRBs), though they may help alleviate symptoms or slow disease progression in some cases. Nanochemistry and peptide-based systems represent breakthrough approaches by leveraging nanoscale precision and biological specificity. This review examines the chemical design and synthesis of nanoparticles (NPs), nanoscaffolds, and peptide conjugates used in retinal neural regeneration. It also explores their biomedical applications, especially in targeted drug delivery, tissue engineering, and cellular repair. Biodegradable polymeric NPs, liposomes, and hybrid nanostructures are designed to cross barriers, release drugs in a controlled manner, and enhance biocompatibility. PEGylation improves stability and reduces immune responses in the ocular environment, while peptide functionalization enables specific cellular targeting and minimizes inflammatory reactions. Peptide-functionalized platforms, such as RGD-modified NPs and self-assembling hydrogels, provide receptor-mediated targeting and extracellular matrix (ECM) mimicry to support retinal regeneration for improved stem cell differentiation and neuroprotection. We discuss drug/gene delivery mechanisms, cellular interactions, and immune modulation, as well as neuroprotection, stem cell therapy, and diagnostic applications. Preclinical studies have demonstrated promising efficacy in animal models; however, concerns regarding scalability, long-term safety, and non-invasive delivery persist. Next-generation technologies, such as stimuli-responsive NPs, computationally designed peptides, and patient-specific delivery systems, are on the horizon to address unmet clinical needs. By marrying nanochemistry's precision with peptides' bioactivity, these technologies have the potential to transform retinal disease treatment, enabling the restoration of vision and an improvement in quality of life for millions of people worldwide.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1235-1262"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145444948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Janus silk fibroin membranes for periodontal-guided tissue regeneration.","authors":"Xiao-Qing Qian, Xue Xu, Meng Zhang, Yu-Qing Zhang, Hai-Yan Wang","doi":"10.1177/08853282251405353","DOIUrl":"10.1177/08853282251405353","url":null,"abstract":"<p><p>Membrane materials containing dense and porous layers are greatly needed for periodontal-guided tissue regeneration (GTR) surgery. Silk fibroin (SF) has been widely used in medical biomaterials. However, conventional methods make it difficult to prepare suitable SF membranes for periodontal GTR. Here, an integrated Janus SF membrane (JSFM)-a membrane with two distinct sides-with dense and porous layers was directly prepared by unidirectional nanopore dehydration (UND) and freeze-drying. The effects of UND duration on the JSFM were examined. In addition, the biocompatibility of the membranes was examined in vitro and in vivo. Scanning electron microscopy showed that the resulting membrane had a Janus structure when the UND was performed for less than 4.5 h. With extended UND duration, the Janus structure disappeared, and the swelling ratio and water uptake abilities of the membranes decreased significantly while the mechanical properties were enhanced. Fourier transform infrared (FTIR) spectroscopy indicated that the crystalline structure of the porous layer gradually increased with increasing UND duration. The in vivo study indicated that the membrane could support the growth and proliferation of human periodontal ligament fibroblast cells (hPDLs), and the dense layer of the membrane effectively prevented the migration of hPDLs. The in vivo study performed in rats demonstrated that the membranes have good biocompatibility. Therefore, a new membrane type with a special Janus structure was developed. The membrane shows excellent biocompatibility and can intercept cells for exploitation in various biomedical applications, particularly in periodontal GTR.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1309-1322"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145604140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The application of biomimetic scaffolds in temporomandibular joint disc regeneration research: Current advances and future challenges.","authors":"Xuelian Su, Guangjie Bao, Jinting Zhang, Jing Wen, Bing Han","doi":"10.1177/08853282261443599","DOIUrl":"https://doi.org/10.1177/08853282261443599","url":null,"abstract":"<p><p>The temporomandibular joint (TMJ) disc is a structurally heterogeneous fibrocartilaginous tissue characterized by sparse vascularity and inherently limited regenerative capacity. Although tissue engineering strategies have made significant progress in promoting TMJ disc regeneration, the resulting neotissue often fails to fully recapitulate the native tissue's biomechanical properties and ultrastructure. To enhance the functional integration of cells with scaffolds and engineer fibrocartilage that closely mimics native structure of the TMJ disc, the development of biomimetic scaffolds capable of recapitulating both structural heterogeneity and anisotropy has emerged as a pivotal focus in tissue regeneration research. This article presents a systematic overview of the current state of application and challenges of biological scaffolds in fibrocartilage regeneration research, focusing on five key domains: micro-mechanically guided anisotropic biomimetic scaffolds, functionally modified decellularized extracellular matrix (dECM), immunomodulatory composite scaffolds, scaffold pore architecture, and spatiotemporally controlled delivery systems. Collectively, these advances lay a robust theoretical foundation and experimental framework for the rational design of biomimetic scaffolds in TMJ disc tissue engineering.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261443599"},"PeriodicalIF":2.5,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147698916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shifting the clinical paradigm: Copper versus silver wound dressings - Where we are and what we are looking for.","authors":"Ithamar Cheyne, Mateusz Rybka, Łukasz Mazurek, Jan Jurak, Marek Konop","doi":"10.1177/08853282251392491","DOIUrl":"10.1177/08853282251392491","url":null,"abstract":"<p><p>The development of nanoparticle-based wound dressings marks a significant advancement in the management of chronic and non-healing wounds. Silver-based dressings have been used in wound management due to their strong antimicrobial properties. However, their clinical effectiveness depends on formulation, concentration, and duration of use. Recently, copper oxide dressings (CODs) have emerged as a novel alternative, offering both antimicrobial and regenerative benefits. We reviewed clinical studies, meta-analyses, and cost-effectiveness analyses on silver nanoparticle (AgNP), ionic silver, nanocrystalline silver, and copper oxide dressings across various wound types, including diabetic foot ulcers, venous leg ulcers, pressure ulcers, surgical wounds, and burns. Emphasis was placed on dressing formulations, silver or copper concentrations, clinical efficacy, safety, and cost-effectiveness. Traditional silver formulations, such as silver sulfadiazine (1%) and silver nitrate (0.5%), demonstrate antimicrobial activity but are limited by cytotoxicity and lack of long-term healing benefits. Nanocrystalline silver and ionic silver hydrofiber dressings provide sustained release, proving most effective in infection-prone and early inflammatory phases. Enhanced formulations (Aquacel® Ag + Extra™) show promise in treating biofilm-related wounds but need more robust data. By contrast, CODs have demonstrated antimicrobial efficacy alongside stimulation of angiogenesis, fibroblast proliferation, and extracellular matrix remodeling. Early clinical evidence suggests that CODs may accelerate healing in refractory wounds and offer cost advantages over negative pressure therapy, though large-scale trials remain limited. Silver dressings, particularly nanocrystalline and ionic hydrofiber formulations, remain clinically useful for infection control and short-term wound management, while older silver salts are less favorable due to toxicity and limited efficacy. CODs represent a biologically attractive alternative with dual antimicrobial and regenerative properties. Nonetheless, the current body of evidence is insufficient to declare a paradigm shift in wound healing, and CODs should presently be regarded as promising adjuncts pending validation in high-quality randomized trials.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1081-1093"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteogenic differentiation by pre-osteoblasts is enhanced more on 3D-PRINTED poly-ɛ-caprolactone scaffolds coated with collagen and hydroxyapatite than on poly-ɛ-caprolactone/hydroxyapatite composite scaffolds coated with collagen.","authors":"Ali Moghaddaszadeh, Mohammad Ehsan Ghiasvand, Hadi Seddiqi, Sonia Abbasi-Ravasjani, Jenneke Klein-Nulend","doi":"10.1177/08853282251392820","DOIUrl":"10.1177/08853282251392820","url":null,"abstract":"<p><p>Three-dimensional (3D)-printed poly-ε-caprolactone (PCL) scaffolds lack sufficient bioactivity for optimal bone tissue engineering applications. This shortcoming can be overcome by coating PCL scaffolds with collagen and hydroxyapatite (PCL/col-HA) or by applying a collagen coating to PCL-HA composite scaffolds (PCL-HA/col). Here we aimed to test which type of scaffold is more effective in stimulating osteogenic activity. Moreover, the scaffolds' physicomechanical properties were characterized. 3D-printed PCL/col-HA containing 10, 20, or 30% HA particles, and 3D-printed PCL-HA/col containing 10, 20, or 30% HA particles with collagen coating were fabricated. MC3T3-E1 pre-osteoblasts were cultured on the scaffolds for 14 days. The physicomechanical properties of the scaffolds and pre-osteoblast functionality were evaluated through experiments and finite element (FE) modeling. We found that coating of PCL scaffolds with collagen and HA or coating of PCL-HA composite scaffolds with collagen changed the geometry and topography of the scaffold surfaces. Furthermore, PCL/col-HA and PCL-HA/col showed higher surface roughness and elastic modulus, but lower water contact angle, than PCL scaffolds. FE-modeling showed that all scaffolds tolerated up to 2% compressive strain, which was lower than their yield stress. 3D-printed PCL/col-HA and PCL-HA/col scaffolds promoted pre-osteoblast proliferation and osteogenic activity compared to unmodified PCL scaffolds. PCL-HA/col scaffolds increased pre-osteoblast proliferation and collagen deposition, whereas PCL/col-HA scaffolds increased alkaline phosphatase activity and calcium deposition. Osteogenic activity of pre-osteoblasts was more enhanced on 3D-printed PCL/col-HA scaffolds than on PCL-HA/col scaffolds, particularly in the short-term, which seems promising for <i>in vivo</i> bone tissue engineering.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1183-1197"},"PeriodicalIF":2.4,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12957415/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145389803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}