Journal of Biomaterials Applications最新文献

A dual-network SIS collagen/chitosan hydrogel with integrated antibacterial and regenerative functions for infected wound repair. 一种集抗菌和再生功能于一体的双网络SIS胶原/壳聚糖水凝胶用于感染伤口修复。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-05 DOI: 10.1177/08853282261449328

Ang Li, Mingwen Jia, Yufei Li, Jiajun Li, Mouwen Qu

{"title":"A dual-network SIS collagen/chitosan hydrogel with integrated antibacterial and regenerative functions for infected wound repair.","authors":"Ang Li, Mingwen Jia, Yufei Li, Jiajun Li, Mouwen Qu","doi":"10.1177/08853282261449328","DOIUrl":"https://doi.org/10.1177/08853282261449328","url":null,"abstract":"The treatment of infected skin wounds remains a significant clinical challenge due to bacterial infection, delayed healing, and the rise of antibiotic-resistant bacteria. This study aims to develop a dual-crosslinked hydrogel dressing with integrated antibacterial and pro-healing functions to promote the regeneration of infected wounds. A dual-network hydrogel (CC/Cu2+ gel) was fabricated through the self-assembly of porcine small intestinal submucosa (SIS) collagen and subsequent ionic crosslinking between chitosan and Cu2+. The material's microstructure, mechanical properties, cytocompatibility, hemocompatibility, and antibacterial activity against S.aureus and E.coli were systematically characterized. The efficacy in promoting infected wound healing was evaluated using a full-thickness skin defect model in Bama miniature pigs. The incorporation of Cu2+ formed a denser polymer network, significantly enhancing the compressive modulus and strength of the hydrogel. The CC/Cu2+-5 gel formulation demonstrated effective antibacterial activity while maintaining acceptable cytocompatibility and hemocompatibility. In vitro, it facilitated fibroblast proliferation, collagen expression, and angiogenesis. In vivo, it accelerated wound closure, achieving a 98.97% healing rate within 24 days, and promoted well-structured epidermal and dermal regeneration, as confirmed by histological and immunofluorescent analyses. The CC/Cu2+ dual-crosslinked hydrogel, leveraging the synergistic effect of SIS collagen and copper ions, represented a promising functional dressing for managing infected wounds.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261449328"},"PeriodicalIF":2.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic enhancement of skull bone regeneration in rats using demineralized dentin matrix dual-loaded with BMP-2 and NELL-1 growth factors. 双负载BMP-2和NELL-1生长因子的脱矿牙本质基质协同促进大鼠颅骨再生。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-05 DOI: 10.1177/08853282261450025

Ruwei Wang, Linlin Xu, Qiang Wang

{"title":"Synergistic enhancement of skull bone regeneration in rats using demineralized dentin matrix dual-loaded with BMP-2 and NELL-1 growth factors.","authors":"Ruwei Wang, Linlin Xu, Qiang Wang","doi":"10.1177/08853282261450025","DOIUrl":"https://doi.org/10.1177/08853282261450025","url":null,"abstract":"To address the limitations associated with current bone graft materials, particularly the slow resorption of deproteinized bovine bone minerals and the adverse effects of high-dose BMP-2, we developed an innovative tripartite regeneration platform. This platform employs a decalcified dentin matrix (DDM) as a physiological carrier for the spatio-temporal co-delivery of BMP-2 and Nell-1, representing a novel combined strategy. The DDM particles, optimized to a size range of 500-1000 μm through EDTA hierarchical decalcification (achieving 70% decalcification), exhibited a marked increase in pulp tubule diameter, cross-sectional area, and porosity. Scanning electron microscopy (SEM) analysis confirmed that the absence of a smear layer and the presence of uniform tubules facilitated osteoblast infiltration. In a rat model with critical-sized skull defects, the synergistic DBN structure (comprising DDM, BMP-2, and NELL-1) resulted in nearly complete bone regeneration within 8 weeks, significantly enhancing bone volume and bone mineral density. This bionic platform addresses the \"bone-induced inflammation paradox\" by utilizing the hierarchical topological structure of DDM and the synergistic dynamics of two factors: the rapid release of BMP-2 and the sustained release of NELL-1. This approach surpasses the clinical gold standard and satisfies the FDA's efficacy criteria for the repair of critical size defects.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261450025"},"PeriodicalIF":2.5,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation into the degradation mechanism of Cu-Fe/HA biodegradable composite materials based on experiments and simulations. 基于实验和模拟的Cu-Fe/HA可生物降解复合材料降解机理研究。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-04 DOI: 10.1177/08853282261447086

Nan Huang, Hong Qin, Siqi Zhao, Yuzhen Feng, Yingyang Zhao, Yingxue Teng

{"title":"Investigation into the degradation mechanism of Cu-Fe/HA biodegradable composite materials based on experiments and simulations.","authors":"Nan Huang, Hong Qin, Siqi Zhao, Yuzhen Feng, Yingyang Zhao, Yingxue Teng","doi":"10.1177/08853282261447086","DOIUrl":"https://doi.org/10.1177/08853282261447086","url":null,"abstract":"The degradation behavior and mechanisms of Cu-Fe/HA composites are investigated in this study, combining experimental analysis with cellular automata simulations to elucidate the synergistic mechanism between Cu and Hydroxyapatite (HA) during the degradation process. The results demonstrate that the corrosion of the Fe matrix exhibits periodic characteristics during corrosion, while the addition of Cu significantly influences the degradation performance of the material. In the early immersion stage (30 days), Cu and Fe form a \"Fe-Cu galvanic cell\", accelerating the corrosion rate of the sample. In the later immersion stage (180 days), Cu is reduced and deposited on the surface to form a protective film, which instead reduces the degradation rate of samples with higher Cu content (as evidenced by the weight loss rates of 0.04046% and 0.04306% for Cu1-Fe and Cu2-Fe, respectively, compared to only 0.01936% for Cu5-Fe) reflecting a dual role of \"initial acceleration followed by subsequent inhibition.\" The introduction of HA further regulates the degradation process. Its preferential degradation leads to the formation of a porous structure in the sample, thereby increasing the contact area between the solution and Fe and accelerating subsequent corrosion of Fe. Combined with cellular automata simulations, the coupling mechanism between corrosion product exfoliation and dynamic Cu deposition was verified, providing theoretical and experimental support for designing controllable-degradation Cu-Fe/HA composite bone implant materials. The results of biological experiments showed that at an extraction concentration of 12.5 wt. %, the bone cell proliferation rate exceeds 100 %, while the hemolysis rate remains below 2 %, meeting the requirements for biomaterials.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261447086"},"PeriodicalIF":2.5,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double emulsion-based encapsulation of iron in amphiphilic block copolymer nanocarriers for controlled release. 铁在两亲嵌段共聚物纳米载体中的双乳化包封控制释放。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-10-31 DOI: 10.1177/08853282251393482

Valentina Camacho, Johan Bermudez, Leon Perez

{"title":"Double emulsion-based encapsulation of iron in amphiphilic block copolymer nanocarriers for controlled release.","authors":"Valentina Camacho, Johan Bermudez, Leon Perez","doi":"10.1177/08853282251393482","DOIUrl":"10.1177/08853282251393482","url":null,"abstract":"In this study, we explored the design of polymeric nanocapsules as vehicles for controlled release of iron. Amphiphilic block copolymers (ABCs) composed of polyethylene glycol (PEG) and poly (ε-caprolactone) (PCL) segments were synthesized via ring-opening polymerization (ROP), using PEG and methoxy-PEG (mPEG) with varying molecular weights as macroinitiators. Structural and molecular characterizations using infrared spectroscopy, proton nuclear magnetic resonance and gel permeation chromatography confirmed successful copolymerization and narrow dispersity indices (Ð <1.5). Iron-loaded nanocapsules were formulated using the double emulsion solvent evaporation (DESE) technique with synthesized PEG-b-PCL copolymers as polymeric precursors. The impact of the copolymer composition on the particle size, morphology, and encapsulation efficiency (EE%) was evaluated. Spherical nanocapsules with diameters below 500 nm were obtained, and a positive correlation was observed between copolymer molecular weight and EE%, with the highest value (74.4%) achieved for the Fe@COP5-96 formulation. Differential scanning calorimetry (DSC) analysis revealed that iron incorporation altered the thermal behavior of the copolymers, resulting in a shift of the melting peaks toward lower temperatures and a decrease in melting enthalpy, consistent with reduced crystallinity arising from ion-polymer interactions. The iron release kinetics exhibited a sustained release behavior. These results demonstrate the potential of PEG-b-PCL nanocapsules as effective carriers for ionic species with promising applications in nutrient delivery and medical therapies.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1263-1273"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation and evaluation of a novel composite hemostatic hydrogel. 一种新型复合止血水凝胶的制备与评价。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-01 DOI: 10.1177/08853282251391262

Feng Shen, Qizun Wang, Ruigang Zhu, Yuelei Wang, Chuqiang Yin, Zengshuai Han, Ting Wang, Yanhui Li

{"title":"Preparation and evaluation of a novel composite hemostatic hydrogel.","authors":"Feng Shen, Qizun Wang, Ruigang Zhu, Yuelei Wang, Chuqiang Yin, Zengshuai Han, Ting Wang, Yanhui Li","doi":"10.1177/08853282251391262","DOIUrl":"10.1177/08853282251391262","url":null,"abstract":"Hemostasis is critical for ensuring surgical success over the past few decades. Various topical hemostatic agents have been developed to promote hemostasis in various surgeries, particularly in cases where traditional surgical techniques are not applicable. However, the hemostatic performance of most agents is often limited by their reliance on a single component. Therefore, it is necessary to develop composite hemostatic agents that integrate multiple materials from diverse sources to enhance hemostatic efficacy. In addition, existing hemostatic agents in solid forms are not often effective in scenarios involving irregularly shaped or deep wounds, as well as endoscopic surgical procedures. In this study, a gelatin-chitosan-thrombin (GCT) composite hemostatic hydrogel was prepared using cross-linking method. The agent's properties, including morphology, water absorption ratio, swelling ratio, and cytotoxicity were systematically evaluated. A rabbit spinal laminectomy model and a rat live injury model were used to evaluate the hemostatic efficacy of GCT agent. Histological assessment was performed to investigate its biocompatibility. The three-dimensional porous structure of the GCT agent endows it with a high absorption capacity and a low swelling ratio. The GCT agent demonstrates superior hemostatic performance in terms of blood loss and bleeding time compared to existing agents in vivo. In addition, the GCT agent exhibits excellent biodegradability and biocompatibility in vivo, and minimal hemolytic and cytotoxic effects in vitro. Therefore, the novel composite hemostatic hydrogel would be a strong candidate for surgical hemostasis especially when precise application is required.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1349-1361"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145421603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D-printed PLA honeycomb-shaped scaffolds for bone tissue engineering. 一种用于骨组织工程的3d打印PLA蜂窝状支架。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-10 DOI: 10.1177/08853282251396800

Aochong Zhou, Junwei Liao, Zhishen Huang, Kaicheng Zeng, Yuying Guo, Xuedan Hou, Hongxia Zhao

{"title":"A 3D-printed PLA honeycomb-shaped scaffolds for bone tissue engineering.","authors":"Aochong Zhou, Junwei Liao, Zhishen Huang, Kaicheng Zeng, Yuying Guo, Xuedan Hou, Hongxia Zhao","doi":"10.1177/08853282251396800","DOIUrl":"10.1177/08853282251396800","url":null,"abstract":"Objectives: Polylactic acid (PLA) is widely used as biomedical material due to its good biocompatibility and biodegradability. A PLA honeycomb-shaped porous scaffold as bone graft substitute was printed by 3D-printed. Method:Coating and mineralization treatment was used in order to further improve the properties of the PLA scaffold. The materials were characterized by infrared spectroscopy (IR) and Xray diffraction (XRD). The structure of the scaffolds was observed by electric scanning microscope (SEM). The hydrophilicity of the material was observed by contact angle tester. Compression tests were carried out to evaluate the strength of the scaffolds. The biocompatibility of the scaffolds was evaluated by MTT. The behaviors and responses of preosteoblast cells on the scaffolds were studied as well. Results: The porosity of the 3D-printed PLA scaffold was 82.6%. The compressive strength and compressive modulus value of the PLA scaffolds was 8.22 ± 0.16 MPa and 244.3 ± 5.7 MPa, respectively. Coating and mineralization treatment could improved the hydrophilicity, strength and the biocompatibility of the scaffold. Conclusions: The 3D-printed PLA porous scaffold has a good prospect for application as artificial scaffold for bone tissue engineering.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1323-1331"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quercetin-loaded MgO nanoparticles in a chitosan/gelatin/PVA matrix enhance KGF1 expression and accelerate wound healing. 壳聚糖/明胶/PVA基质中负载槲皮素的MgO纳米颗粒可增强KGF1的表达并加速伤口愈合。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-17 DOI: 10.1177/08853282251399589

Najmeh Darvishi, Somayeh Reiisi, Sadegh Shirian

{"title":"Quercetin-loaded MgO nanoparticles in a chitosan/gelatin/PVA matrix enhance KGF1 expression and accelerate wound healing.","authors":"Najmeh Darvishi, Somayeh Reiisi, Sadegh Shirian","doi":"10.1177/08853282251399589","DOIUrl":"10.1177/08853282251399589","url":null,"abstract":"Effective treatment of skin wounds is essential due to the skin's protective, regulatory, and aesthetic functions. Post-injury infections can significantly impair healing, highlighting the need for advanced biomaterials that combine antimicrobial activity with regenerative potential. In this study, we developed a multifunctional chitosan/gelatin/polyvinyl alcohol (CS/GEL/PVA) nanocomposite containing magnesium oxide (MgO) nanoparticles loaded with quercetin (MgO@QC), aimed at enhancing wound healing and promoting keratinocyte growth factor 1 (KGF1) expression. MgO nanoparticles were synthesized and characterized using DLS, zeta potential, FTIR, XRD, FESEM, and TEM. Quercetin was successfully loaded onto the MgO nanoparticles with a high loading efficiency of 99%, as confirmed by spectroscopic analyses. The resulting nanocomposite demonstrated favorable physicochemical properties, including uniform morphology, excellent swelling behavior (∼79%), optical clarity, and robust structural integrity. Hemolysis assays revealed excellent hemocompatibility, while in vitro cytotoxicity tests confirmed biocompatibility up to 500 µg/mL. Cell proliferation and migration assays (MTT and scratch test) showed dose-dependent enhancement of fibroblast activity, particularly at 1 mg/mL. The nanocomposite also significantly upregulated KGF1 gene expression, suggesting its role in stimulating epithelial regeneration. In vivo studies using a murine excisional wound model demonstrated accelerated wound closure and tissue regeneration in the MgO@QC-treated group, supported by histological evidence of angiogenesis, re-epithelialization, and reduced inflammation. The CS/GEL/PVA/MgO@QC nanocomposite offers a biocompatible and bioactive platform that significantly enhances wound healing. These findings suggest its strong potential for clinical application as an advanced wound dressing for acute and chronic skin injuries.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1332-1348"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145540563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Verification of renal tissue regeneration using human perinephric fat-derived collagen sheet in a partially nephrectomized animal model. 人肾周脂肪源性胶原蛋白片在部分肾切除动物模型中的肾组织再生验证。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-17 DOI: 10.1177/08853282251399548

Bum Soo Kim, Eun Hye Lee, Bo Hyun Yoon, Minji Jeon, Jun-Koo Kang, Jae-Wook Chung, Phil Hyun Song, Tae Gyun Kwon, Yun-Sok Ha

{"title":"Verification of renal tissue regeneration using human perinephric fat-derived collagen sheet in a partially nephrectomized animal model.","authors":"Bum Soo Kim, Eun Hye Lee, Bo Hyun Yoon, Minji Jeon, Jun-Koo Kang, Jae-Wook Chung, Phil Hyun Song, Tae Gyun Kwon, Yun-Sok Ha","doi":"10.1177/08853282251399548","DOIUrl":"10.1177/08853282251399548","url":null,"abstract":"Chronic kidney disease (CKD), a global health issue, affects approximately 10% of the population. However, limited treatment options, such as dialysis or transplantation, have significant drawbacks. Therefore, this study aims to investigate the potential of a collagen sheet derived from human perirenal adipose tissue for kidney regeneration. Collagen sheets were derived from discarded perirenal adipose tissues and implanted into partially nephrectomized mice. The right kidneys were completely removed, and 2 mm of the upper and lower poles of the left kidneys were resected. A collagen sheet measuring 1 × 1 × 3 mm3 was implanted in the mid-pole of the left kidney following partial resection of renal parenchyme. Renal function, inflammation, and tissue regeneration were evaluated using serum analysis, PCR, histological staining, and immunohistochemistry to assess structural and functional improvements. The collagen sheet reduced pro-inflammatory markers, minimized fibrosis, and restored renal function indicators such as BUN and cystatin C, though creatinine levels remained unchanged. Regenerative markers, including PAX2 and Wt1, were significantly elevated, indicating enhanced tissue repair and structural recovery. The perirenal adipose tissue-derived collagen sheet demonstrated anti-inflammatory effects and promoted renal tissue regeneration. These findings suggest its potential as a biomaterial for renal injury management. However, further research is needed to evaluate long-term efficacy, optimize application methods, and ensure clinical safety.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1377-1387"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145540784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological and biochemical characterization of novel dual-layer amnion and full-thickness perinatal tissue wound care allografts. 新型双层羊膜和全层围产期组织创面异体移植的组织学和生化特征。

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-11 DOI: 10.1177/08853282251398626

Emily S DiNicola, Jerry Chang, Jeremy J Mercuri

{"title":"Histological and biochemical characterization of novel dual-layer amnion and full-thickness perinatal tissue wound care allografts.","authors":"Emily S DiNicola, Jerry Chang, Jeremy J Mercuri","doi":"10.1177/08853282251398626","DOIUrl":"10.1177/08853282251398626","url":null,"abstract":"Aims: Processed perinatal tissue allografts have emerged as adjunctive treatment options for chronic wounds. Different processing techniques used to manufacture perinatal tissue allografts can substantially alter their material and biochemical properties. Thus, the aim of this study was to perform multi-scale characterizations of a dual-layer amnion and full-thickness amnion/chorion allograft. Methods: Histological and biochemical techniques were used to evaluate the extracellular matrix (ECM) microarchitecture and composition of a dual-layer amnion and a full-thickness amnion/chorion allograft. Established assays were performed to quantify graft sulfated glycosaminoglycan (sGAG), collagen, growth factor, and cytokine content. In vitro cellular responses, including proliferation, metabolic activity, and migration of human dermal fibroblasts (HDFs) was used to assess bioactivity of graft extracts. Results: Histological analysis of dual-layer amnion and full-thickness amnion/chorion grafts demonstrated preservation of native ECM layers containing intact cell nuclei, GAGs, collagen, and elastin. sGAG and collagen content of the grafts were comparable to native tissue values reported in literature. Angiogenic, regenerative, matrix remodeling, immunomodulatory, and neurotrophic growth factors were found in dual-layer amnion and full-thickness amnion/chorion grafts. Both grafts induced a significant increase in metabolic activity of HDFs compared to negative controls. Conclusion: Dual-layer amnion and full-thickness amnion/chorion wound care allografts are comprised of an intact microarchitecture containing a variety of ECM components that can provide bioactive signals to HDFs.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1362-1376"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of salt leaching process parameters on the morphology and properties of 3D porous PLA and PEG/PLA scaffolds. 盐浸工艺参数对三维多孔聚乳酸和聚乙二醇/聚乳酸支架形态和性能的影响

IF 2.5 4区医学

Journal of Biomaterials Applications Pub Date : 2026-05-01 Epub Date: 2025-11-13 DOI: 10.1177/08853282251397732

Ioanna-Georgia Athanasoulia, Petroula A Tarantili

{"title":"Effect of salt leaching process parameters on the morphology and properties of 3D porous PLA and PEG/PLA scaffolds.","authors":"Ioanna-Georgia Athanasoulia, Petroula A Tarantili","doi":"10.1177/08853282251397732","DOIUrl":"10.1177/08853282251397732","url":null,"abstract":"In this study, porous PLA structures were prepared using the porogen leaching technique, specifically with sodium chloride (NaCl) of particle sizes 200-300 µm and 400-500 µm, and polyethylene glycol (PEG) with molecular weights of 3,000, 6,000, or 10,000 g/mol. Scanning electron microscopy (SEM) characterization of the cross-sections revealed that larger NaCl particle sizes contributed to an increased degree of pore connectivity, while PEG with the lowest molecular weight accelerated the leaching process. As the concentration of NaCl in the polymeric matrix increased, its removal became more effective, as indicated by lower residual percentages during thermogravimetric analysis (TGA). Additionally, lower residual percentages were recorded for the systems containing PEG prior to leaching. Although the average diameter of the resulting pores decreased in systems that used PEG, the porous structure achieved was more uniform, with both micro- and macro-porosity observed on the surfaces of the scaffold cross-sections. This variation in pore geometry is desirable and can be tailored for specific applications in scaffold construction for tissue engineering. Water exposure altered the inherent properties of PLA, affecting its suitability for short-term, soft-tissue compatible scaffolds. Solution viscometry revealed a molecular weight drop to contribute to accelerated biodegradation. Differential Scanning Calorimetry (DSC) showed a decrease in the glass transition temperature (Tg), and in cold crystallization temperature (Tcc). In addition, the thermal degradation resistance of PLA decreased, as determined by TGA experiments. The aforementioned changes were significantly amplified in PLA specimens subjected to dual leaching of both PEG and NaCl.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1286-1308"},"PeriodicalIF":2.5,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145512941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0