Journal of Biomaterials Applications最新文献

Treatment of bacterial keratitis in mouse using Ag nanoparticles suspension. 银纳米颗粒悬浮液治疗小鼠细菌性角膜炎。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-05-28 DOI: 10.1177/08853282261456461

Chang Liu, Zhezhen Wei, Lanlan Wang, Qing Zhang, Dongwei Liu

{"title":"Treatment of bacterial keratitis in mouse using Ag nanoparticles suspension.","authors":"Chang Liu, Zhezhen Wei, Lanlan Wang, Qing Zhang, Dongwei Liu","doi":"10.1177/08853282261456461","DOIUrl":"10.1177/08853282261456461","url":null,"abstract":"This study aimed to develop a novel topical treatment for Pseudomonas aeruginosa (P. aeruginosa)-induced bacterial keratitis (BK) using silver nanoparticles (Ag NPs), addressing critical limitations of current antibiotics, such as poor ocular penetration and microbial resistance. Ag NPs were synthesized via silver-cysteine conjugation and characterized by UV-Vis spectroscopy, zeta potential, dynamic light scattering (DLS), TEM, XRD, and XPS. In vitro, the nanoparticles exhibited potent antibacterial activity against P. aeruginosa with minimal cytotoxicity toward human corneal epithelial cells (HCECs). Notably, they also retained observable activity against methicillin-resistant Staphylococcus aureus (MRSA), suggesting potential for addressing drug-resistant infections. In a mouse model of P. aeruginosa-induced bacterial keratitis, topical application of the Ag NPs effectively ameliorated the infection without harming healthy ocular tissues, demonstrating favorable biocompatibility and strong therapeutic potential. Overall, Ag NPs represent a promising topical nanotherapeutic for BK, offering enhanced ocular bioavailability, retained activity against drug-resistant strains, and favorable biocompatibility, highlighting their strong potential for clinical translation in ocular infection management.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"732-743"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148054639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface modification of keratin films via thiol-maleimide chemistry toward enhanced anticoagulant response. 巯基-马来酰亚胺化学对角蛋白膜表面进行修饰以增强抗凝反应。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-06-10 DOI: 10.1177/08853282261460783

Kazuya Sawada, Toshiya Fujisato

{"title":"Surface modification of keratin films via thiol-maleimide chemistry toward enhanced anticoagulant response.","authors":"Kazuya Sawada, Toshiya Fujisato","doi":"10.1177/08853282261460783","DOIUrl":"10.1177/08853282261460783","url":null,"abstract":"Protein-based biomaterials are widely used in tissue engineering and regenerative medicine; however, many conventional materials suffer from limitations including immunogenicity, low stability, and risks associated with animal-derived sources. In this context, keratin derived from human hair has attracted increasing attention due to its inherent biocompatibility and cysteine-rich structure, which provides reactive thiol (-SH) groups suitable for chemical modification. In the present study, reductively extracted keratin was utilized to preserve thiol functionality, enabling site-selective modification via thiol-maleimide chemistry using an aminoethyl maleimide (AEM) linker. Through this approach, heparin, a clinically established anticoagulant, was immobilized onto keratin films under mild aqueous conditions. The resulting keratin-heparin films exhibited effective suppression of thrombin-induced coagulation, whereas unmodified keratin showed no anticoagulant activity. Furthermore, spectroscopic and colorimetric analyses confirmed successful heparin conjugation and surface presentation, and no measurable heparin release was detected, indicating stable surface immobilization. Overall, these results demonstrate that thiol-specific functionalization provides a selective, stable, and animal-free platform for anticoagulant surface modification, highlighting the potential of keratin-based materials for blood-contacting biomedical applications.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"700-718"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148217427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-network SIS collagen/chitosan hydrogel with integrated antibacterial and regenerative functions for infected wound repair. 一种集抗菌和再生功能于一体的双网络SIS胶原/壳聚糖水凝胶用于感染伤口修复。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-05-05 DOI: 10.1177/08853282261449328

Ang Li, Mingwen Jia, Yufei Li, Jiajun Li, Mouwen Qu

{"title":"A dual-network SIS collagen/chitosan hydrogel with integrated antibacterial and regenerative functions for infected wound repair.","authors":"Ang Li, Mingwen Jia, Yufei Li, Jiajun Li, Mouwen Qu","doi":"10.1177/08853282261449328","DOIUrl":"10.1177/08853282261449328","url":null,"abstract":"The treatment of infected skin wounds remains a significant clinical challenge due to bacterial infection, delayed healing, and the rise of antibiotic-resistant bacteria. This study aims to develop a dual-crosslinked hydrogel dressing with integrated antibacterial and pro-healing functions to promote the regeneration of infected wounds. A dual-network hydrogel (CC/Cu2+ gel) was fabricated through the self-assembly of porcine small intestinal submucosa (SIS) collagen and subsequent ionic crosslinking between chitosan and Cu2+. The material's microstructure, mechanical properties, cytocompatibility, hemocompatibility, and antibacterial activity against S.aureus and E.coli were systematically characterized. The efficacy in promoting infected wound healing was evaluated using a full-thickness skin defect model in Bama miniature pigs. The incorporation of Cu2+ formed a denser polymer network, significantly enhancing the compressive modulus and strength of the hydrogel. The CC/Cu2+-5 gel formulation demonstrated effective antibacterial activity while maintaining acceptable cytocompatibility and hemocompatibility. In vitro, it facilitated fibroblast proliferation, collagen expression, and angiogenesis. In vivo, it accelerated wound closure, achieving a 98.97% healing rate within 24 days, and promoted well-structured epidermal and dermal regeneration, as confirmed by histological and immunofluorescent analyses. The CC/Cu2+ dual-crosslinked hydrogel, leveraging the synergistic effect of SIS collagen and copper ions, represented a promising functional dressing for managing infected wounds.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"744-756"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cu-doped MOF-based nanocomposites with acidity-triggerable aggregation for precise photothermal/chemokinetic combined therapy. 用于精确光热/趋化动力学联合治疗的酸触发聚集铜掺杂mof基纳米复合材料。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-05-10 DOI: 10.1177/08853282261448031

Xuejiao Ma, Yunhan Huang, Xinyu Wang, Jingrui Chang, Xiangru Xu, Aolin Chen, Bo Lu

引用次数: 0

Keratin-based electrospun nanofibers for drug delivery and tissue engineering applications. 角蛋白基静电纺纳米纤维在药物输送和组织工程中的应用。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-05-25 DOI: 10.1177/08853282261454295

Bipasha Biswas, Gita Mishra

{"title":"Keratin-based electrospun nanofibers for drug delivery and tissue engineering applications.","authors":"Bipasha Biswas, Gita Mishra","doi":"10.1177/08853282261454295","DOIUrl":"10.1177/08853282261454295","url":null,"abstract":"Keratin-based electrospun nanofibers have gained increasing attention as promising biomaterials for drug delivery and tissue engineering due to their intrinsic biocompatibility, bioactivity, and controlled biodegradability. Keratin is rich in functional groups and natural cell-recognition motifs, enabling favorable cell-material interactions, including enhanced adhesion, proliferation, and differentiation. When processed into nanofibrous scaffolds via electrospinning, keratin can closely replicate the nanoscale architecture of the native extracellular matrix, offering high surface area and interconnected porosity that support therapeutic loading and tissue regeneration. This review critically summarizes recent progress in the fabrication of keratin-based electrospun nanofibers, with particular emphasis on electrospinning parameters, keratin-polymer blending strategies, and surface modification approaches used to tailor fiber morphology, mechanical properties, and degradation behavior. Methods for incorporating small-molecule drugs, proteins, growth factors, and nanoparticles are discussed, highlighting their influence on encapsulation efficiency, release profiles, and biological performance. Key biomedical applications are reviewed, including wound healing, skin and soft-tissue repair, bone and cartilage regeneration, and localized cancer therapy, where keratin nanofibers have demonstrated improved healing outcomes, reduced infection, and enhanced tissue integration compared with conventional biomaterial systems. Current challenges related to keratin source variability, limited mechanical strength for load-bearing applications, scalability, and regulatory translation are analyzed. Finally, future perspectives are outlined, focusing on hybrid and stimuli-responsive keratin-based nanofibers and sustainable processing strategies, underscoring their potential as clinically relevant platforms for advanced biomaterials applications.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"611-631"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148015081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tannic acid-metal single-cell nano-coatings regulate bone mesenchymal stem cell fate through ion-specific pericellular microenvironments. 单宁酸-金属单细胞纳米涂层通过离子特异性细胞周围微环境调节骨间充质干细胞的命运。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-06-03 DOI: 10.1177/08853282261449941

Jia Wu, Renguo Gong, Yongmei Wen, Shan Kong, Pan Yang, Xia Deng

{"title":"Tannic acid-metal single-cell nano-coatings regulate bone mesenchymal stem cell fate through ion-specific pericellular microenvironments.","authors":"Jia Wu, Renguo Gong, Yongmei Wen, Shan Kong, Pan Yang, Xia Deng","doi":"10.1177/08853282261449941","DOIUrl":"10.1177/08853282261449941","url":null,"abstract":"Bone and cartilage regeneration remains a major clinical challenge due to the limited intrinsic repair capacity of musculoskeletal tissues. Biomaterial strategies capable of regulating stem cell behavior at the single-cell level offer new opportunities for precise control of tissue regeneration. Herein, we developed tannic acid-metal (TA-metal) coordination systems incorporating Fe3+, Sr2+, and Mg2+ ions as single-cell nano-coatings to modulate the pericellular microenvironment of bone marrow-derived mesenchymal stem cells (BMSCs). The physicochemical properties of the TA-metal coatings, including protein adsorption, surface morphology, surface charge, ion release behavior, and elemental composition, were systematically characterized. All TA-metal systems formed conformal coatings on individual cells with excellent cytocompatibility and showed detectable retention during early culture. The TA-metal nano-coatings significantly regulated cell morphology and cytoskeletal organization in a metal ion-dependent manner. Notably, TA-Sr and TA-Mg coatings promoted enhanced cell spreading and well-organized actin structures. Tri-lineage differentiation assays revealed distinct lineage-specific regulatory effects. TA-Fe nano-coatings preferentially enhanced adipogenic differentiation, whereas TA-Sr coatings significantly promoted chondrogenic matrix production while supporting osteogenic differentiation. TA-Mg nano-coatings exhibited the strongest osteogenic potential, as evidenced by increased alkaline phosphatase activity and extracellular matrix mineralization. Collectively, these results demonstrate that TA-metal single-cell nano-coatings enable programmable, ion-specific regulation of stem cell fate within an identical coordination framework. This cell-level biomaterial strategy provides a versatile platform for skeletal tissue engineering and cell-based regenerative therapies.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"686-699"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148149888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sericin-based biomaterial for craniomaxillofacial regeneration: Preclinical evidence and translational potential. 丝胶基生物材料用于颅颌面再生：临床前证据和转化潜力。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-06-06 DOI: 10.1177/08853282261459552

Łukasz Mazurek, Marek Konop, Fernando P S Guastaldi

{"title":"Sericin-based biomaterial for craniomaxillofacial regeneration: Preclinical evidence and translational potential.","authors":"Łukasz Mazurek, Marek Konop, Fernando P S Guastaldi","doi":"10.1177/08853282261459552","DOIUrl":"10.1177/08853282261459552","url":null,"abstract":"Significant craniomaxillofacial (CMF) bone defects after trauma, tumor removal, or congenital disabilities are a major clinical challenge. Therefore, new methods are continually sought to improve treatment effectiveness. In this context, next-generation biomaterials deserve special attention. Sericin, a protein component of silk, demonstrates regenerative potential. Current evidence suggests that sericin actively stimulates osteogenesis by promoting the differentiation of osteoprogenitor cells and upregulating key markers, including Runx2, osteocalcin, and osteopontin. In addition, it exhibits a favorable biocompatibility profile and promising immunomodulatory properties. By exerting anti-inflammatory effects, sericin may promote a pro-healing polarization of M2 macrophages and reduce TNF-α expression. The antimicrobial potential of sericin-based biomaterials is also highlighted, though the evidence remains debated and further validation is needed, particularly for grafts and scaffolds used in CMF regeneration. Sericin also appears to promote angiogenesis and the organized deposition of mineralized extracellular matrix, which are crucial factors in bone regeneration. In preclinical animal models, sericin-based biomaterials have shown statistically significant improvements in the healing of calvarial, alveolar, and long bone defects. Despite these encouraging findings, the translation of sericin into clinical practice remains limited, with only two human studies available, primarily for minor applications in oral surgery. This review summarizes the current literature focusing on sericin-based biomaterials and relates the available evidence to potential applications in CMF regeneration. In a structured manner, it begins with the physical and biological properties of sericin, proceeds to cellular and tissue interactions, and presents preclinical and clinical evidence, critically evaluating the advantages and limitations.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"632-644"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148176036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic enhancement of skull bone regeneration in rats using demineralized dentin matrix dual-loaded with BMP-2 and NELL-1 growth factors. 双负载BMP-2和NELL-1生长因子的脱矿牙本质基质协同促进大鼠颅骨再生。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-05-05 DOI: 10.1177/08853282261450025

Ruwei Wang, Linlin Xu, Qiang Wang

{"title":"Synergistic enhancement of skull bone regeneration in rats using demineralized dentin matrix dual-loaded with BMP-2 and NELL-1 growth factors.","authors":"Ruwei Wang, Linlin Xu, Qiang Wang","doi":"10.1177/08853282261450025","DOIUrl":"10.1177/08853282261450025","url":null,"abstract":"To address the limitations associated with current bone graft materials, particularly the slow resorption of deproteinized bovine bone minerals and the adverse effects of high-dose BMP-2, we developed an innovative tripartite regeneration platform. This platform employs a decalcified dentin matrix (DDM) as a physiological carrier for the spatio-temporal co-delivery of BMP-2 and Nell-1, representing a novel combined strategy. The DDM particles, optimized to a size range of 500-1000 μm through EDTA hierarchical decalcification (achieving 70% decalcification), exhibited a marked increase in pulp tubule diameter, cross-sectional area, and porosity. Scanning electron microscopy (SEM) analysis confirmed that the absence of a smear layer and the presence of uniform tubules facilitated osteoblast infiltration. In a rat model with critical-sized skull defects, the synergistic DBN structure (comprising DDM, BMP-2, and NELL-1) resulted in nearly complete bone regeneration within 8 weeks, significantly enhancing bone volume and bone mineral density. This bionic platform addresses the \"bone-induced inflammation paradox\" by utilizing the hierarchical topological structure of DDM and the synergistic dynamics of two factors: the rapid release of BMP-2 and the sustained release of NELL-1. This approach surpasses the clinical gold standard and satisfies the FDA's efficacy criteria for the repair of critical size defects.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"645-658"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis, characterization and biological performance of a calcium titanium oxide film prepared by sol-gel/dip coating technique on titanium. 溶胶-凝胶/浸渍法制备氧化钛钙膜的合成、表征及生物性能研究。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-05-21 DOI: 10.1177/08853282261454303

J I M M Garcia, C J C Fernandes, W M Silva, M C Rossi, V B Amigó, W F Zambuzzi, M J Saeki

{"title":"Synthesis, characterization and biological performance of a calcium titanium oxide film prepared by sol-gel/dip coating technique on titanium.","authors":"J I M M Garcia, C J C Fernandes, W M Silva, M C Rossi, V B Amigó, W F Zambuzzi, M J Saeki","doi":"10.1177/08853282261454303","DOIUrl":"10.1177/08853282261454303","url":null,"abstract":"In this study, coatings based on calcium titanate (CT) were applied onto titanium substrates using sol-gel process combined with dip-coating. In addition to physical and chemical characterization, we evaluated the impact of this surface modification on the expression of key genes involved in cell adhesion, extracellular matrix (ECM) remodeling, and early osteogenic commitment in pre-osteoblasts (MC3t3-E1, Subclone 4). X-ray diffraction (XRD) confirmed the presence of cubic alpha-calcium titanate (CaTiO3) strongly bonded to the titanium surface. Although the coating reduced surface roughness, high dipping speeds induced the formation of thicker layers with microcracks due to thermal expansion mismatches. Increased dipping speeds, however, improved surface wettability. Electrochemical analysis revealed enhanced corrosion behavior, attributed to the formation of a stable oxide layer. Biological assays (MTT and crystal violet) confirmed the absence of cytotoxic effects in both direct and indirect contact conditions. Gene expression analysis showed significant upregulation of Itgb1, Fak, Col1a1, and Runx2 in cells cultured with conditioned media from CT-coated surfaces, indicating enhanced integrin-mediated adhesion, ECM deposition, and early osteogenic differentiation. These molecular responses were accompanied by cytoskeletal remodeling, evidenced by increased phosphorylation of cofilin, suggesting enhanced actin filament stabilization. Collectively, sol-gel CT-based layers exhibit beneficial properties as surface coatings and promote a favorable microenvironment for cell adhesion, matrix remodeling, and osteogenic signaling, without inducing cytotoxicity, underscoring their potential for biomedical applications.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"671-685"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147987599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graphene oxide-enhanced dissolvable microneedles for transdermal delivery of alendronate: Augmented permeation and antibacterial activity. 用于经皮给药阿仑膦酸钠的氧化石墨烯增强可溶微针：增强渗透和抗菌活性。

IF 2.4 4区医学

Journal of Biomaterials Applications Pub Date : 2026-11-01 Epub Date: 2026-06-02 DOI: 10.1177/08853282261457811

Qianqian Sun, Xiaowen Li, Guofa Zhang, Bing Li, Yanlian Niu, Litao Wang, Mei Lv

{"title":"Graphene oxide-enhanced dissolvable microneedles for transdermal delivery of alendronate: Augmented permeation and antibacterial activity.","authors":"Qianqian Sun, Xiaowen Li, Guofa Zhang, Bing Li, Yanlian Niu, Litao Wang, Mei Lv","doi":"10.1177/08853282261457811","DOIUrl":"10.1177/08853282261457811","url":null,"abstract":"Osteoporosis treatment using alendronate (ALN) is limited by poor oral bioavailability and gastrointestinal side effects. To address this limitation, this study developed a graphene oxide (GO)-enhanced soluble microneedle (MN) system for transdermal administration of ALN to treat osteoporosis. The MNs were fabricated from polyvinyl alcohol (PVA) and polyvinylpyrrolidone (PVP) using a two-step casting method, forming a drug-loaded core-barrier outer layer. The incorporation of GO significantly improved mechanical strength, with penetration efficiency reaching 66∼88%, and enhanced swelling capacity (PP/GO-ALN swelling ratio: 299%). In vitro release studies showed no significant difference between PP-ALN and PP/GO-ALN MNs in a dialysis bag model, while ex vivo skin permeation demonstrated that PP/GO-ALN MNs achieved significantly higher cumulative drug permeation (1069.53 µg/cm2) over 24 h compared to PP-ALN MNs (712.89 µg/cm2). Furthermore, GO conferred notable antibacterial activity, and the PP/GO-ALN formulation synergistically promoted osteoblast proliferation (cell viability: 107.42%, p < 0.05). These findings demonstrate that the GO-ALN MN system possesses mechanical properties, transdermal delivery, antibacterial effects, and biocompatibility. It provides a highly promising non-invasive strategy for the treatment of osteoporosis.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"659-670"},"PeriodicalIF":2.4,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148149870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0