Journal of Biomaterials Applications最新文献

{"title":"Towards oral insulin-controlled release nanomedicine: A review.","authors":"Ayana Kannaghut Puthukudi, Kaladhar Kamalasanan, Prajitha Prabhakaran Ganga, Harika Sapa, Shona Sara Shaji, Sreejith Thrivikraman, Althaf Umar, Sherin Ann, Shine Sadasivan, Praveen Vp, Mishra Narayan Chandra, Hani Harb, Manish Kumar Jeengar","doi":"10.1177/08853282251383026","DOIUrl":"https://doi.org/10.1177/08853282251383026","url":null,"abstract":"<p><p>Frequent insulin injections remain the primary method for regulating blood glucose levels in individuals with diabetes mellitus; however, patient compliance is often poor. Due to its non-invasive nature, oral insulin delivery, exploring nanomedicine strategies, is considered a highly desirable alternative as an affordable and accessible medicine. However, the physical intestinal barriers and the harsh gastrointestinal environment provide major obstacles to reaching the best possible pharmacological bioavailability of insulin. Insulin's stability, bioavailability, and targeted administration throughout the GI tract can be improved using colloidal nanocarriers, including polymeric nanoparticles, phospholipid vesicles, and lipid-based nanoparticles. These nanocarriers mimic the physiological insulin secretion and improve the pharmacokinetics of insulin by shielding it from enzymatic degradation, facilitating controlled release, and enhancing absorption across the intestinal mucosa. Key parameters such as particle size, surface charge, zeta potential, and polymer-mucin interactions are examined concerning their effects on epithelial transport and enzymatic protection. Strategies such as PEGylation, chitosan functionalization, and bile salt incorporation are discussed with an emphasis on their interfacial engineering potential. Additionally, novel strategies such as glucose-responsive formulations, cell-penetrating peptides, and enzyme inhibitors, and innovative devices like microneedle capsules and SOMA systems have been explored to enhance oral insulin efficacy. This might not, however, be helpful for translation on its own. Another deciding aspect will be the combination of that with distinct pathways. Future perspectives and innovative approaches to enhance the therapeutic potential of nano-driven systems for oral insulin administration are also discussed in this review as an affordable and accessible medicine strategy.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251383026"},"PeriodicalIF":2.5,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel injectable tricalcium silicate composite bone cement with built-in antibacterial protection.","authors":"Azadeh Saberi, Amir Moeintaghavi, Hossein Bagheri, Sahar Mollazadeh Beidokhti, Mohammad Jalili Nik","doi":"10.1177/08853282251379731","DOIUrl":"https://doi.org/10.1177/08853282251379731","url":null,"abstract":"<p><p>This investigation examines the influence of calcium sulfate (CaS) and modified nano-hydroxyapatite (mHA) additions on the physicochemical properties, microstructural development, apatite-forming potential, and antibacterial properties of bioactive tricalcium silicate (C₃S) cement. Although C₃S cements exhibit inherent antibacterial properties, their efficacy in treating infected bone defects requires enhancement. The release kinetics of vancomycin (VANCO), an antibiotic, and the modified cements' antibacterial efficacy were systematically evaluated. The findings revealed a notable decrease in setting time from 363 to 264 min upon the integration of CaS. The composite cements demonstrated flow properties and injectability that met standard requirements, exceeding 75% at both 2 and 5 min. The modified cements noted Improved compressive strength compared to their unmodified counterparts. Furthermore, the cements promoted the formation of apatite on their surfaces when immersed in phosphate-buffered saline (PBS). Antibacterial evaluations established that VANCO released from the composites effectively impeded bacterial proliferation. These findings suggest that C₃S cement enhanced with CaS and mHA exhibits superior physicochemical characteristics and bioactivity, thereby establishing it as a promising candidate for cutting-edge bone repair materials.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251379731"},"PeriodicalIF":2.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altering the pH during sol-gel synthesis of bioactive glass particles and its effect on structural and antibacterial properties.","authors":"Danielle L Perry, Anthony W Wren","doi":"10.1177/08853282251386622","DOIUrl":"https://doi.org/10.1177/08853282251386622","url":null,"abstract":"<p><p>In sol-gel glass chemistry, the pH of the sol directly influences the rate of the hydrolysis and condensation reactions, leading to changes in the glass's structural properties and potentially altering its function as a biomaterial. This research used various acidic pH values, 2, 3, 3.65, 5, and 5.65, to create sol-gel bioactive glass with a 45SiO₂-14.5NaO₂-14.5CaO-6P₂O₅-10ZnO-5CuO-5CoO mol% composition. A pH of 2 allowed for increased surface area, 26.23 m²/g, and cumulative surface area of pores, 34.78 m²/g, compared to the other pH values used. Raman spectroscopy highlighted variances in the intensity of Q² and Q³ species, with a pH of 2 and 3.65 having a higher intensity of Q³ species. Inductively coupled plasma-optical emission spectroscopy (ICP-OES) revealed that the concentration of Cu²⁺ ions released from the glass network in simulated body fluid (SBF) was the highest after 1000 h of incubation for the pH 3.65 glass, 100 mg/L, which translated to the most significant inhibition of <i>E. coli</i> after 48 h of contact. Elemental, thermal, and structural analysis using energy dispersive X-ray spectroscopy, differential thermal analysis, Fourier-Transform Infrared Spectroscopy, and X-ray diffraction was also performed, with no discernible relationship found between changing the pH of the sol used to synthesize these glasses.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251386622"},"PeriodicalIF":2.5,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145251196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucoadhesive micelles for ophthalmic drug delivery.","authors":"Taylor Goostrey, Mitchell Ross, Karim Soliman, Lindsay Sheardown, Heather Sheardown","doi":"10.1177/08853282251386004","DOIUrl":"https://doi.org/10.1177/08853282251386004","url":null,"abstract":"<p><p>The most common formulation for treating ocular conditions is topical eyedrops, despite their well-documented inefficiency. In this study, mucoadhesive nano-micelles were developed to overcome the poor efficacy of topical eyedrops in the treatment of dry eye disease. The micelles contained a pre-activated thiomer capable of releasing mucolytic N-acetylcysteine upon covalent disulfide exchange with the natural mucus layer which covers the surface of the eye. The micelles, approximately 70 nm in diameter, were shown to be mucoadhesive through zeta potential analysis. The critical micelle concentration was determined to be 217 mg/L using the pyrene fluorescence method. The core of the micelles was loaded with cyclosporine A, displaying a greater than 90% entrapment efficiency, and yielding sustained release of approximately 57% over 10 days. The cellular response to the micelles was tested with human corneal epithelial cells by MTT assay and Live/Dead staining. It was found that lower concentrations of the amphiphilic polymer resulted in greater cellular viability and in all cases, viability increased from 24 to 48 h following treatment. Overall, these mucoadhesive systems have potential to provide more efficacious treatment of anterior segment ocular conditions.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251386004"},"PeriodicalIF":2.5,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145244640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and utilization of a novel nanoantimicrobial suture for surgery: An in vivo and mechanical study.","authors":"Yeliz Kılınç, İnci Rana Karaca, Aysel Uğur, Sibel Elif Gültekin, İpek Atak Seçen, Nurdan Saraç, Leyla Arslan Bozdağ, Tuba Baygar","doi":"10.1177/08853282251383088","DOIUrl":"https://doi.org/10.1177/08853282251383088","url":null,"abstract":"<p><p>Development of surgical sutures coated with antimicrobial agents is a promising strategy to minimize surgical site infection (SSI) and improve wound healing. The antimicrobial features of <i>Hypericum Perforatum</i> and biogenic silver nanoparticles (AgNPs) have arised an increasing demand for processing surgical sutures. Herein the results of the animal experiments and mechanical tests of a novel antimicrobial silk suture coated with <i>H. perforatum</i> extract (Hp) and biogenic AgNPs (Hp-AgNP) are reported. The study used in vivo histological, histochemical, and immunohistochemical techniques to illustrate the variations in inflammatory response, re-epithelialization, and collagenization of the coated silk sutures in a rat buccal mucosa incision model. Diameter, knot-pull tensile strength, knot security, tie-down, and needle attachment tests were carried out for evaluating the effects of the coating process on mechanical and handling properties. Histopathological and immunohistochemical evaluations revealed progressive healing in all groups, with variations in wound closure, inflammation, and cytokine expression. Hp-AgNP-coated sutures showed significant improvements in re-epithelialization and reduced TNF-α and IL-6 levels over time, highlighting their potential benefits in enhancing wound healing compared to other materials. The coating process had a remarkable effect on the mechanical and handling properties. Coated sutures exhibited higher values than control groups. Suture diameter, knot-pull tensile strength and knot security revealed the highest values for Hp-AgNP-coated suture. The Hp-AgNP coating on the silk suture significantly improves wound healing, mechanical and handling properties. This implies that it has the potential to be a feasible substitute for commercially available silk sutures in surgical interventions. (Scheme 1).</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251383088"},"PeriodicalIF":2.5,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145212731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-hydroxyapatite doped tyramine gelatin/silk fibroin scaffold for the regeneration of cancellous bone defects.","authors":"Lina Yang, Mengting Wang, Caixing Peng, Xiang Gong, Lihong Fan, Shuhua Liu, Shengxiang Tao","doi":"10.1177/08853282251347346","DOIUrl":"10.1177/08853282251347346","url":null,"abstract":"<p><p>Gelatin (G) and silk fibroin (SF) are well-established as scaffold materials for bone regeneration; however, their limited binding abilities and mechanical properties often result in less-than-ideal outcomes. In this study, we sought to enhance the stability of a silk fibroin/gelatin biomimetic scaffold by introducing a tyramine bond to the gelatin and incorporating nanohydroxyapatite as a bioactive element. This innovation led to the development of a more robust silk fibroin/nano-hydroxyapatite/gelatin tyramine biomimetic scaffold (SHGT). The biomimetic scaffold was fabricated through an enzymatic reaction catalyzed by horseradish peroxidase/hydrogen peroxide (HRP/H₂O₂), which facilitated the interaction between a high concentration of silk fibroin (17%) and gelatin tyramine (GT). Additionally, nano-hydroxyapatite (nHA) was incorporated as a bioactive filler to promote bone repair. Our findings indicated that the SHG biomimetic scaffold, initially designed as a sponge, was transformed into an SHGT scaffold with improved brittle fracture resistance, thus broadening its potential applications in bone reconstruction. Moreover, the data showed that combining GT with RGD sequences and HA as a bioactive component significantly enhanced the viability of bone marrow stromal cells (BMSCs) cultured on the scaffold. This synergistic effect highlights the potential of the SHGT scaffold as a promising material for bone tissue engineering.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"500-512"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing gelatin hydrogels: Synergistic effects of C-phycocyanin and nopal mucilage on physicochemical and biocompatibility properties.","authors":"Emma Gabriela Antonio-Marcos, Hugo Joel Carrillo Escalante, Liliana Hernández-Vázquez, Gerardo Alfonso Castillo Gamboa, José Manuel Cervantes Uc, Jesús Alejandro Claudio Rizo, Nayeli Rodríguez-Fuentes","doi":"10.1177/08853282251355114","DOIUrl":"10.1177/08853282251355114","url":null,"abstract":"<p><p>Healing persistent wounds is a current challenge for healthcare systems. Addressing this type of problem requires new and improved materials that activate regenerative processes without side effects. In this sense, in this study, C-phycocyanin (CPC), a bioactive pigment obtained from <i>Arthrospira platensis</i>, and nopal mucilage (MUC), a traditional Mexican element of ancestral medicine, were incorporated into gelatin (GEL)-based hydrogels and chemically crosslinked. These materials, referred to as HGEL-CPC-MUC, were prepared with varying concentrations of CPC-MUC (0-1 μg/μL of hydrogel), and their structural, physicochemical, rheological and <i>in</i> <i>vitro</i> biocompatibility properties were systematically evaluated. The main findings revealed that the incorporation of CPC-MUC into GEL-based hydrogels, significantly improves their physicochemical, mechanical and biological properties. These hydrogels exhibited a chemical crosslinking, achieving 93% crosslinking efficiency, high swelling behavior (∼1250%), rough porous surfaces, sustained degradation at physiological pH, and high thermal stability. Their rheological behavior showed an improvement in G' (226%) under thermal stress (40 °C), along with high damping capacity under constant load with the addition of CPC-MUC. Notably, the presence of CPC-MUC imparted a hemoprotective effect, with hemolysis percentages decreasing proportionally to the CPC-MUC content and none of the hydrogels interfered with coagulation pathways. Furthermore, all hydrogels demonstrated excellent <i>in</i> <i>vitro</i> biocompatibility with dermal fibroblasts, showing no cytotoxic effects. These features become important in the context of a moist and refractory wounds such as foot ulcers and extensive burns, were moisture control, exceptional hemocompatibility and support for dermal fibroblasts viability are required, as well as the porous structure for nutrients and waste exchange. HGEL-CPC-MUC hydrogels represent a highly promising biocompatible and multifunctional scaffold for advanced wound care and regenerative medicine applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"529-546"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an enhanced liver scaffold recellularization using fibronectin.","authors":"Sadia Afrin, Usha Yadav, Chandra J Yadav, Jihad Kamel, Jun-Young Lee, Kyung-Mee Park","doi":"10.1177/08853282251350315","DOIUrl":"10.1177/08853282251350315","url":null,"abstract":"<p><p>Decellularized liver scaffolds offer a promising foundation for liver tissue engineering and regenerative medicine. However, several challenges such as poor cell adhesion, inefficient reseeding, inadequate vascularization, and a high risk of blood clot formation continue to hinder their clinical application. While fibronectin (FN) has been widely used to enhance scaffold functionality, its potential for liver-specific applications remains largely unexplored. In this study, we developed a perfusion-assisted FN coating technique to improve the adhesion of endothelial cells (EA.hy926) and hepatocytes (HepG2), thereby enhancing the overall biocompatibility of liver scaffolds. FN was carefully introduced into decellularized rat liver scaffolds, allowing for targeted deposition across both the vascular and parenchymal compartments to optimize cellular attachment. Following portal vein reseeding and 7 days of bioreactor incubation, the FN-coated scaffolds showed significantly better endothelial cell adhesion within blood vessel structures and increased HepG2 cell coverage throughout the liver tissue. Immunohistochemistry (IHC) confirmed enhanced HepG2 proliferation, while TUNEL and RT-qPCR analyses indicated improved cell viability and scaffold functionality. Additionally, ex vivo blood perfusion tests demonstrated reduced thrombogenicity, likely due to improved endothelialization and lower platelet adhesion. These findings highlight FN functionalization as an effective bioengineering approach to overcoming key barriers in vascularization, biocompatibility, and cellular integration for liver scaffolds. By extending the known benefits of FN beyond its previously studied applications in kidney and heart scaffolds, this research introduces a promising strategy for advancing bioengineered liver grafts and potential transplantation models.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"513-528"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on fabrication of calcium sulfate hemihydrate/tricalcium phosphate based injectable bone cement modified by sodium alginate-carboxymethyl chitosan gel network and its resistance to collapse.","authors":"Junjia Kang, Xiaojie Lian, Zhimin He, Tingwei Qin, Di Huang","doi":"10.1177/08853282251361217","DOIUrl":"10.1177/08853282251361217","url":null,"abstract":"<p><p>The collapsibility of bone cement may cause blood vessel embolism, blocking blood flow and causing serious complications such as pulmonary embolism or spinal cord injury, especially when implantation by injection. Therefore, it is of great significance to develop an artificial bone graft with excellent collapse resistance performance. Calcium sulfate and calcium phosphate complex bone cements can be formulated as injectable materials, making them particularly suitable for treating irregular bone defects. However, its clinical application is limited by poor collapsibility resistance and mechanical strength. This study aimed to develop an injectable bone repair material by integrating a biphasic calcium source, which was achieved by calcium sulfate (CS) and calcium phosphate (CP), and a synergistic network formed by sodium alginate (SA) and carboxymethyl chitosan (CMCS). The results showed that the addition of SA-CMCS as a solidifying liquid significantly improved the compressive strength, injectability, and collapsibility resistance of composite bone cement. At the concentration of 1% SA and 15% CMCS, the peak compressive strength reached 11.53 ± 1.3 MPa. All the composite bone cements did not collapse at 5 h in the static environment, and the collapse times of samples SA1-CMCS15 and SA1-CMCS20 in the dynamic environment were 95.3 ± 5.1 min and 96.7 ± 4.9 min, respectively. At CMCS concentrations of 10-20%, the injectability of composite bone cement was higher than 90% and degradation ratio was less than 15%. ALP activity and alizarin red staining confirmed that the composite bone cement showed excellent cytocompatibility and promoted cell proliferation and osteogenic differentiation. This study successfully developed a bone repair material with enhanced mechanical properties, collapsibility resistance, injectability, and biocompatibility, which may make it a promising candidate for bone regeneration applications in clinical.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"547-559"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Fiber-reinforced hydrogel scaffolds for heart valve tissue engineering.","authors":"","doi":"10.1177/08853282251340963","DOIUrl":"10.1177/08853282251340963","url":null,"abstract":"","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"560-561"},"PeriodicalIF":2.5,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}