Journal of Biomaterials Applications最新文献

Analysis of foreign body response and systemic toxicity of additively manufactured nanocellulose reinforced alginate gelatin-based scaffolds with interconnected 3D porous structure. 增材制备纳米纤维素增强海藻酸盐明胶三维互联多孔支架的异物反应及全身毒性分析。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-03 DOI: 10.1177/08853282251330744

Sulob Roy Chowdhury, Bikramjit Basu

{"title":"Analysis of foreign body response and systemic toxicity of additively manufactured nanocellulose reinforced alginate gelatin-based scaffolds with interconnected 3D porous structure.","authors":"Sulob Roy Chowdhury, Bikramjit Basu","doi":"10.1177/08853282251330744","DOIUrl":"10.1177/08853282251330744","url":null,"abstract":"The last two decaes have witnessed significant efforts to develop gelatin/alginate based scaffolds using variants of 3D printing techniques. However, their biocompatibility for regenerating complex soft tissues remains insufficiently explored. Addressing this gap, we fabricated 3D-printed alginate-gelatin (3A5G) and nanocellulose-reinforced (3A5G1C) hydrogel scaffolds with clinically relevant dimensions (15 mm diameter, 5 mm height) and the host tissue responses were critically analyzed. The distinct advantages of nanocellulose in modulating mechanical strength, viscoelasticity, swelling, and degradation characteristics were established in our prior studies. This investigation aimed to comprehensively evaluate the foreign body response of these scaffolds in a rat model. The animals exhibited healthy metabolic activity, evidenced by progressive weight gain, localized tissue healing, and normal mobility over 30 days. Histological analyses could not reveal any adverse immune reaction at 7- or 30-days, post-implantation. Hematological and serum biochemical assessments indicated a progression from acute (7 days) to sub-acute (30 days) inflammation, following subcutaneous implantation, without any signature of systemic toxicity. Immune marker evaluation (TNF-α, CD-8, CD-68, COX-2, IL-6) confirmed the absence of pathological immune responses, even with nanocellulose incorporation. Immunohistochemical analysis using CD31 staining demonstrated enhanced vascularization in nanocellulose-reinforced scaffolds at both 7 and 30 days. The absence of systemic toxicity from scaffold degradation products and the favorable biocompatibility outcomes underline the potential of these hydrogel scaffolds for soft tissue regeneration. The incorporation of nanocellulose further enhanced the scaffolds' functional performance, particularly in promoting vascularization, positioning them as promising candidates for complex tissue engineering applications.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"192-217"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium nanoparticles and paclitaxel co-delivery by a PCL based nanofibrous scaffold to enhance melanoma therapy. 硒纳米颗粒和紫杉醇共递送PCL纳米纤维支架增强黑色素瘤治疗。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-03-28 DOI: 10.1177/08853282251330724

Maryam Doostan, Ata'ollah Rahmani Azar, Hassan Maleki

{"title":"Selenium nanoparticles and paclitaxel co-delivery by a PCL based nanofibrous scaffold to enhance melanoma therapy.","authors":"Maryam Doostan, Ata'ollah Rahmani Azar, Hassan Maleki","doi":"10.1177/08853282251330724","DOIUrl":"10.1177/08853282251330724","url":null,"abstract":"The incidence of skin cancer has increased significantly in recent decades, highlighting the need for more effective treatments due to the limitations of traditional approaches. This study focused on creating a poly (ε-caprolactone) and chitosan (PCL/CS) nanofibrous scaffold loaded with selenium nanoparticles (Se NPs) and paclitaxel (PTX) to inhibit melanoma cell growth. The synthesized Se NPs, characterized by their uniform spherical shape and nano-scale size (∼120 nm), were incorporated into the scaffold. Then, the Se NPs and PTX were concurrently loaded into PCL/CS nanofibers at 5 wt%, which resulted in fibers with an average diameter of 253 ± 35 nm, presenting a ribbon-like morphology and absence of droplets/beads. The results indicated a high fluid absorption capacity, a wettability and high tensile strength of the produced scaffold. Moreover, the controlled release of the loaded compounds was provided over several days. Remarkably, high toxicity (>90%) and higher levels of apoptosis (>85%) were observed in A375 melanoma cells treated with the PTX-Se NPs PCL/CS scaffold. Moreover, the assessment of fibroblast growth and hemolysis confirmed the scaffold's high level of biocompatibility. The PTX-Se NPs PCL/CS nanofibers exhibit favorable properties and strong anti-tumor efficacy, making them a promising scaffold for localized and selective chemotherapy in anti-melanoma treatment.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"165-180"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coacervation-responsive cubosome containing hyaluronic acid and albumin complex. 含有透明质酸和白蛋白复合物的凝聚反应性立方体。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-15 DOI: 10.1177/08853282251334466

Hyeon Ki Son, Panalee Pomseethong, Jin-Chul Kim

{"title":"Coacervation-responsive cubosome containing hyaluronic acid and albumin complex.","authors":"Hyeon Ki Son, Panalee Pomseethong, Jin-Chul Kim","doi":"10.1177/08853282251334466","DOIUrl":"10.1177/08853282251334466","url":null,"abstract":"Coacervation-responsive cubosomes were prepared by loading a complex of hydrophobically modified hyaluronic acid (HmHA) and hydrophobically modified albumin (HmAlb) and steviol glycoside (SG) into the water channels. Hyaluronic acid and albumin were modified with a lipid chain, and the HmHA and HmAlb were characterized by 1H NMR and FT-IR spectroscopy, respectively. The formation of the HmHA/HmAlb coacervate complex was optimized when the mass ratio was 1:9 under pH 4.0 conditions. The phase transition temperature of the cubic phase complex was observed to increase slightly from 60.9°C to 61.6°C as a result of the inclusion of the coacervate complex, as evidenced by differential scanning calorimetry. The maximum release degree of SG at 22°C was suppressed to 30.9% due to the coacervate at pH 3, and it was promoted to 75.9% at pH 5.5 due to the dissolution of the electrostatic complex as the pH value increased. The monoolein of the cubosDome enhanced the in vitro skin permeation of the cubosomal SG, as it could play a role as a skin permeation enhancer. The coacervation-responsive cubosome could be potentially used as a drug carrier that can release its content in a pH-controlled manner.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"252-267"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of processing conditions on the physical-chemical and mechanical properties of chitosan-alginate polyelectrolyte complex films for potential wound dressing application. 加工条件对壳聚糖-海藻酸盐聚电解质复合膜的物理化学和力学性能的影响。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-14 DOI: 10.1177/08853282251334472

Andressa de Espíndola Sobczyk, Pietra Ferreria Aguiar, Débora Jung Luvizetto Faccin, Nilo Sérgio Medeiros Cardozo, Isabel Cristina Tessaro

{"title":"Effect of processing conditions on the physical-chemical and mechanical properties of chitosan-alginate polyelectrolyte complex films for potential wound dressing application.","authors":"Andressa de Espíndola Sobczyk, Pietra Ferreria Aguiar, Débora Jung Luvizetto Faccin, Nilo Sérgio Medeiros Cardozo, Isabel Cristina Tessaro","doi":"10.1177/08853282251334472","DOIUrl":"10.1177/08853282251334472","url":null,"abstract":"The combination of chitosan and alginate leads to the formation of polyelectrolyte complexes (PECs) that have been mainly used for applications such as wound dressings in biomedical areas. However, processing conditions can affect the resulting complex structure, influencing the final material properties. This work aims to evaluate the influence of processing conditions on the physical-chemical and mechanical properties of chitosan-alginate PEC films for wound dressing applications. The study was carried out using a Box-Behnken design, with controlled variables including pH, agitation speed, amounts of crosslinker and plasticizer, and the type of acid used in chitosan solubilization. Response variables were thickness, liquid absorption capacity, water vapor barrier, and mechanical properties, which are important characteristics in defining the applicability of dressings. All studied factors, as well as their interactions, showed significant effects on the properties of interest. The mathematical models obtained for the studied properties did not have a predictive character but rather a qualitative one, providing a good insight into the behavior of these materials regarding the modification of the evaluated experimental conditions, which strongly influence the characteristics of chitosan-alginate PEC films. Additional swelling and FTIR analyses performed for a selected sub-set of samples confirmed, respectively: (i) the high equilibrium values and stability at the equilibrium of the films regarding liquid absorption for both water and PBS; (ii) no degradation of the chitosan and alginate functional groups or loss of interaction between them under the considered processing conditions.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"236-251"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a ROS-responsive, glutathione-functionalized injectable hydrogel system for controlled drug release. 开发一种ros反应，谷胱甘肽功能化的可注射水凝胶系统，用于控制药物释放。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-10 DOI: 10.1177/08853282251334208

Kai Hu, Linlin Liang, Jian Song

{"title":"Development of a ROS-responsive, glutathione-functionalized injectable hydrogel system for controlled drug release.","authors":"Kai Hu, Linlin Liang, Jian Song","doi":"10.1177/08853282251334208","DOIUrl":"10.1177/08853282251334208","url":null,"abstract":"Oxidative stress arises from an imbalance between excessive production of reactive oxygen species (ROS) and the body's antioxidant defenses. In neurodegenerative diseases, this imbalance leads to ROS accumulation, causing neuronal dysfunction and cell death. Traditional drug therapies often fail to address the dynamic nature of neuroinflammation, limiting their therapeutic efficacy. To overcome this challenge, we have developed an innovative ROS-responsive injectable hydrogel. This hydrogel is designed to detect oxidative stress sensitively and release glutathione in a controlled manner, thereby modulating inflammation and restoring the damaged immune microenvironment to facilitate tissue repair. The hydrogel was synthesized by crosslinking polyvinyl alcohol (PVA) with sodium alginate modified with 3-aminophenylboronic acid (Alg-PBA). We investigated the hydrogel's formation mechanism and analyzed how component variations affect its morphological and rheological properties. Our findings demonstrate that an optimal Alg-PBA to PVA weight ratio of 2:1 yields a hydrogel with superior mechanical strength. Glutathione (GSH) release studies confirmed the hydrogel's pronounced ROS-responsive drug release behavior. Furthermore, biocompatibility assessments revealed that the hydrogel loaded with 100 μg/mL GSH exhibited excellent compatibility and significantly inhibited neuronal apoptosis under oxygen-glucose deprivation (OGD) conditions. This work presents a promising strategy for treating inflammation-related diseases and provides valuable insights for designing next-generation hydrogels that adapt to injury-responsive microenvironments.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"181-191"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A numerical study on mechanical and permeability properties of novel design additive manufactured Titanium based metal matrix composite bone scaffold for bone tissue engineering. 新设计的增材制造钛基金属基复合骨支架的力学性能和渗透性能的数值研究。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-15 DOI: 10.1177/08853282251333237

Umanath Puthillam, Renold Elsen Selvam

{"title":"A numerical study on mechanical and permeability properties of novel design additive manufactured Titanium based metal matrix composite bone scaffold for bone tissue engineering.","authors":"Umanath Puthillam, Renold Elsen Selvam","doi":"10.1177/08853282251333237","DOIUrl":"10.1177/08853282251333237","url":null,"abstract":"A novel design was developed for extrusion based additive manufacturing (robocasting) of bone scaffolds and a numerical study was carried out to find the optimal design to develop a bone scaffold for critical bone defect treatments. Initially, Representative Volume Analysis (RVE) analysis was carried out to predict the Young's modulus (E) of Titanium + Calcium Silicate and Titanium + Hydroxyapatite composites. The RVE analysis outputs were used to find out the E value of various bone scaffold designs and material compositions. The novel stepped design could be used to tailor the mechanical and biological properties of the scaffold by altering the contact support area between strands and changing the pore size, shape and orientation to control the permeability and nutrient transportation. The test revealed that some of the designed scaffolds are suitable for developing scaffolds for cortical bone defects as the E value lies between 10 and 30 GPa. The CFD analysis indicated that some designs do not possess the permeability required for a scaffold to aid nutrient transportation which is ideally between 1.5 × 10-9 and 5 × 10-8 m2. A sample model was printed and sintered in an argon atmosphere using a microwave furnace to check the feasibility of the process.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"268-283"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel two-step conversion from DCPD-coated β-TCP to low crystallinity β-TCP porous scaffolds via combination between dry heating and hydrothermal methods: Effects on pre-osteoblast cell responses. 干热与水热相结合，从dcpd包被β-TCP到低结晶度β-TCP多孔支架的新型两步转化：对成骨前细胞反应的影响。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-08 DOI: 10.1177/08853282251333231

Ahmed Hafedh Mohammed Mohammed, Khairul Anuar Shariff, Mohamad Hafizi Abu Bakar, Ali A Salman, Aira Matsugaki, Takayoshi Nakano, Intan Nirwana, Alexander Patera Nugraha

{"title":"A novel two-step conversion from DCPD-coated β-TCP to low crystallinity β-TCP porous scaffolds via combination between dry heating and hydrothermal methods: Effects on pre-osteoblast cell responses.","authors":"Ahmed Hafedh Mohammed Mohammed, Khairul Anuar Shariff, Mohamad Hafizi Abu Bakar, Ali A Salman, Aira Matsugaki, Takayoshi Nakano, Intan Nirwana, Alexander Patera Nugraha","doi":"10.1177/08853282251333231","DOIUrl":"10.1177/08853282251333231","url":null,"abstract":"This study presents a novel two-step process to fabricate low crystallinity (LC) β-tricalcium phosphate (β-TCP) porous scaffolds and evaluates their implications for pre-osteoblast cell responses. The novelty of this study lies in the two-step conversion of dicalcium phosphate dihydrate (DCPD) -coated β-TCP porous scaffold into LC β-TCP porous scaffolds through a combination of dry heating and hydrothermal conditions at 200°C. The obtained LC β-TCP porous scaffolds were characterised using a Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD), Fourier-Transform Infrared (FTIR), porosity, and compressive strength analysis confirmed the successful fabrication of LC β-TCP scaffolds. Besides, in vitro tests using pre-osteoblast MC3T3-E1 cells were conducted to investigate the cell responses toward LC β-TCP porous scaffolds. The results revealed that the LC β-TCP porous scaffolds were successfully fabricated by converting the DCPD-coated β-TCP into the dicalcium phosphate anhydrous (DCPA) coated β-TCP, followed by a hydrothermal process in a 0.1 mol/L calcium chloride (CaCl2) aqueous solution at 200°C for 24 hours to obtain LC of pure β-TCP scaffold. Moreover, in vitro cell study indicated that the cell density and proliferation surrounding the surface of the LC β-TCP porous scaffold were greater than DCPD-coated β-TCP porous scaffolds. The findings from this study are expected to significantly impact bioceramic technology by enhancing cell responses.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"218-235"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory and antioxidant effects of haematococcus carbon dots in ulcerative colitis: A nanoparticle-based approach. 碳红球菌点在溃疡性结肠炎中的抗炎和抗氧化作用：一种基于纳米粒子的方法。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-13 DOI: 10.1177/08853282251333240

Yuting Liu, Mengqing Wang, Chaoyan Zhang

引用次数: 0

3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects. 3d打印纳米羟基磷灰石/聚（乳酸-羟基乙酸）脂肪源间充质干细胞支架增强骨缺损大鼠骨再生

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-03 DOI: 10.1177/08853282251332050

Hai Yan, Fei Xia

{"title":"3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects.","authors":"Hai Yan, Fei Xia","doi":"10.1177/08853282251332050","DOIUrl":"10.1177/08853282251332050","url":null,"abstract":"Three-dimensional (3D) printing has huge potential in the medical field, including bone tissue engineering scaffolds. In our study, the 3D-printed poly (lactic-co-glycolic acid) (PLGA)/nano-hydroxyapatite (HA) scaffolds carrying adipose-derived mesenchymal stem cells (ADMSCs) were constructed, and whether such scaffolds have therapeutic potential in bone defects was investigated. For in vitro assays, rat ADMSCs were implanted into blank cell wells (Blank) and PLGA/nHA and ADMSCs/PLGA/nHA scaffolds. The vitality and proliferation of ADMSCs were detected through calcein-AM/PI staining and CCK-8 assay to assess the biocompatibility of the scaffolds. ADMSCs in three groups were incubated in osteogenic induction medium, and ALP and ARS staining were performed after 7 days and 21 days, respectively. Runx2, Osterix, OCN, and OPN mRNA expression in ADMSCs was detected through RT-qPCR. For in vivo assays, rat models of radius defects were implanted by PLGA/nHA scaffolds or ADMSCs/PLGA/nHA scaffolds, and micro-CT scan analysis was conducted at week 12 after implantation. Bone marrow cell formation and Runx2 expression in rat radius tissues were evaluated through H&E and immunohistochemical staining, respectively. The results showed that ADMSCs/PLGA/nHA scaffolds provided transplanted cells with a stable carrier as well as maintained their activity and facilitated their proliferation. ADMSCs/PLGA/nHA promoted ADMSC osteogenic differentiation in vitro. Besides, the implantation of ADMSCs/PLGA/nHA scaffolds improved bone regeneration, enhanced bone marrow cell formation, and increased Runx2 expression in rat models of radius defects. Collectively, the 3D-printed ADMSCs/PLGA/nHA scaffolds effectively promote ADMSC osteogenic differentiation and exhibit significant bone repair effects, suggesting its therapeutical potential for bone defects.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"284-296"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk fibroin-hyaluronic acid nanofibers for peripheral nerve regeneration. 丝素-透明质酸纳米纤维用于周围神经再生。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-03-25 DOI: 10.1177/08853282251329315

Madeline R Badrak, Judy Senanayake, Ahmad Zunnu Rain, Harini G Sundararaghavan

{"title":"Silk fibroin-hyaluronic acid nanofibers for peripheral nerve regeneration.","authors":"Madeline R Badrak, Judy Senanayake, Ahmad Zunnu Rain, Harini G Sundararaghavan","doi":"10.1177/08853282251329315","DOIUrl":"10.1177/08853282251329315","url":null,"abstract":"Peripheral nerve injuries are common and a major source of pain that can lead to debilitating loss of function. Current treatments are limited, with autologous nerve grafts being the gold standard treatment for nerve injuries. However, autografting is not always successful and can lead to increased debilitation through donor site morbidity. Tissue engineering seeks to improve nerve injury treatment though the use of nerve conduits. Conduits made from a functional biomaterial can be implanted into a nerve injury site encouraging and controlling axonal regrowth without causing additional harm to the patient. Both silk fibroin (SF) and hyaluronic acid (HA) have been proven successful in the field of neural tissue engineering. SF has excellent mechanical properties and is biocompatible. HA is part of the extracellular matrix and had been used in nerve regeneration applications. This study developed aligned combination SF-HA nanofibers through electrospinning that could be used within a nerve conduit. Both materials were methacrylated to allow for photocrosslinking and additional control over material properties. Methcrylated SF-HA was tested alongside a material containing only methacrylated HA that has already proven to be effective in literature. When characterizing the materials, it was found that through chemical methacrylation HA was substituted at 60% while SF reported a 30% substitution. Electrospun SF-HA nanofibers were found to have a greater diameter than HA fibers; however, SF-HA was found to be more aligned with greater surface hydrophobicity. Mechanically, it was found that both materials exceeded the elastic modulus of native tissue, but SF-HA far exceeded HA in elasticity and overall fiber extension. Furthermore, human Schwann cells attached, proliferated, and released more pro-regenerative growth factors on SF-HA than HA. Dorsal root ganglia neurons also displayed longer neurite extensions on SF-HA fibers. We concluded that SF-HA nanofibers have potential as a nerve conduit material.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"307-323"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0