{"title":"3d打印纳米羟基磷灰石/聚（乳酸-羟基乙酸）脂肪源间充质干细胞支架增强骨缺损大鼠骨再生","authors":"Hai Yan, Fei Xia","doi":"10.1177/08853282251332050","DOIUrl":null,"url":null,"abstract":"<p><p>Three-dimensional (3D) printing has huge potential in the medical field, including bone tissue engineering scaffolds. In our study, the 3D-printed poly (lactic-co-glycolic acid) (PLGA)/nano-hydroxyapatite (HA) scaffolds carrying adipose-derived mesenchymal stem cells (ADMSCs) were constructed, and whether such scaffolds have therapeutic potential in bone defects was investigated. For <i>in vitro</i> assays, rat ADMSCs were implanted into blank cell wells (Blank) and PLGA/nHA and ADMSCs/PLGA/nHA scaffolds. The vitality and proliferation of ADMSCs were detected through calcein-AM/PI staining and CCK-8 assay to assess the biocompatibility of the scaffolds. ADMSCs in three groups were incubated in osteogenic induction medium, and ALP and ARS staining were performed after 7 days and 21 days, respectively. Runx2, Osterix, OCN, and OPN mRNA expression in ADMSCs was detected through RT-qPCR. For <i>in vivo</i> assays, rat models of radius defects were implanted by PLGA/nHA scaffolds or ADMSCs/PLGA/nHA scaffolds, and micro-CT scan analysis was conducted at week 12 after implantation. Bone marrow cell formation and Runx2 expression in rat radius tissues were evaluated through H&E and immunohistochemical staining, respectively. The results showed that ADMSCs/PLGA/nHA scaffolds provided transplanted cells with a stable carrier as well as maintained their activity and facilitated their proliferation. ADMSCs/PLGA/nHA promoted ADMSC osteogenic differentiation <i>in vitro</i>. Besides, the implantation of ADMSCs/PLGA/nHA scaffolds improved bone regeneration, enhanced bone marrow cell formation, and increased Runx2 expression in rat models of radius defects. Collectively, the 3D-printed ADMSCs/PLGA/nHA scaffolds effectively promote ADMSC osteogenic differentiation and exhibit significant bone repair effects, suggesting its therapeutical potential for bone defects.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251332050"},"PeriodicalIF":2.3000,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects.\",\"authors\":\"Hai Yan, Fei Xia\",\"doi\":\"10.1177/08853282251332050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three-dimensional (3D) printing has huge potential in the medical field, including bone tissue engineering scaffolds. In our study, the 3D-printed poly (lactic-co-glycolic acid) (PLGA)/nano-hydroxyapatite (HA) scaffolds carrying adipose-derived mesenchymal stem cells (ADMSCs) were constructed, and whether such scaffolds have therapeutic potential in bone defects was investigated. For <i>in vitro</i> assays, rat ADMSCs were implanted into blank cell wells (Blank) and PLGA/nHA and ADMSCs/PLGA/nHA scaffolds. The vitality and proliferation of ADMSCs were detected through calcein-AM/PI staining and CCK-8 assay to assess the biocompatibility of the scaffolds. ADMSCs in three groups were incubated in osteogenic induction medium, and ALP and ARS staining were performed after 7 days and 21 days, respectively. Runx2, Osterix, OCN, and OPN mRNA expression in ADMSCs was detected through RT-qPCR. For <i>in vivo</i> assays, rat models of radius defects were implanted by PLGA/nHA scaffolds or ADMSCs/PLGA/nHA scaffolds, and micro-CT scan analysis was conducted at week 12 after implantation. Bone marrow cell formation and Runx2 expression in rat radius tissues were evaluated through H&E and immunohistochemical staining, respectively. The results showed that ADMSCs/PLGA/nHA scaffolds provided transplanted cells with a stable carrier as well as maintained their activity and facilitated their proliferation. ADMSCs/PLGA/nHA promoted ADMSC osteogenic differentiation <i>in vitro</i>. Besides, the implantation of ADMSCs/PLGA/nHA scaffolds improved bone regeneration, enhanced bone marrow cell formation, and increased Runx2 expression in rat models of radius defects. Collectively, the 3D-printed ADMSCs/PLGA/nHA scaffolds effectively promote ADMSC osteogenic differentiation and exhibit significant bone repair effects, suggesting its therapeutical potential for bone defects.</p>\",\"PeriodicalId\":15138,\"journal\":{\"name\":\"Journal of Biomaterials Applications\",\"volume\":\" \",\"pages\":\"8853282251332050\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-04-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomaterials Applications\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1177/08853282251332050\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, BIOMEDICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomaterials Applications","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/08853282251332050","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects.
Three-dimensional (3D) printing has huge potential in the medical field, including bone tissue engineering scaffolds. In our study, the 3D-printed poly (lactic-co-glycolic acid) (PLGA)/nano-hydroxyapatite (HA) scaffolds carrying adipose-derived mesenchymal stem cells (ADMSCs) were constructed, and whether such scaffolds have therapeutic potential in bone defects was investigated. For in vitro assays, rat ADMSCs were implanted into blank cell wells (Blank) and PLGA/nHA and ADMSCs/PLGA/nHA scaffolds. The vitality and proliferation of ADMSCs were detected through calcein-AM/PI staining and CCK-8 assay to assess the biocompatibility of the scaffolds. ADMSCs in three groups were incubated in osteogenic induction medium, and ALP and ARS staining were performed after 7 days and 21 days, respectively. Runx2, Osterix, OCN, and OPN mRNA expression in ADMSCs was detected through RT-qPCR. For in vivo assays, rat models of radius defects were implanted by PLGA/nHA scaffolds or ADMSCs/PLGA/nHA scaffolds, and micro-CT scan analysis was conducted at week 12 after implantation. Bone marrow cell formation and Runx2 expression in rat radius tissues were evaluated through H&E and immunohistochemical staining, respectively. The results showed that ADMSCs/PLGA/nHA scaffolds provided transplanted cells with a stable carrier as well as maintained their activity and facilitated their proliferation. ADMSCs/PLGA/nHA promoted ADMSC osteogenic differentiation in vitro. Besides, the implantation of ADMSCs/PLGA/nHA scaffolds improved bone regeneration, enhanced bone marrow cell formation, and increased Runx2 expression in rat models of radius defects. Collectively, the 3D-printed ADMSCs/PLGA/nHA scaffolds effectively promote ADMSC osteogenic differentiation and exhibit significant bone repair effects, suggesting its therapeutical potential for bone defects.
期刊介绍：
The Journal of Biomaterials Applications is a fully peer reviewed international journal that publishes original research and review articles that emphasize the development, manufacture and clinical applications of biomaterials.
Peer-reviewed articles by biomedical specialists from around the world cover:
New developments in biomaterials, R&D, properties and performance, evaluation and applications
Applications in biomedical materials and devices - from sutures and wound dressings to biosensors and cardiovascular devices
Current findings in biological compatibility/incompatibility of biomaterials
The Journal of Biomaterials Applications publishes original articles that emphasize the development, manufacture and clinical applications of biomaterials. Biomaterials continue to be one of the most rapidly growing areas of research in plastics today and certainly one of the biggest technical challenges, since biomaterial performance is dependent on polymer compatibility with the aggressive biological environment. The Journal cuts across disciplines and focuses on medical research and topics that present the broadest view of practical applications of biomaterials in actual clinical use.
The Journal of Biomaterial Applications is devoted to new and emerging biomaterials technologies, particularly focusing on the many applications which are under development at industrial biomedical and polymer research facilities, as well as the ongoing activities in academic, medical and applied clinical uses of devices.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
