Journal of Biomaterials Applications最新文献_第2页

A novel two-step conversion from DCPD-coated β-TCP to low crystallinity β-TCP porous scaffolds via combination between dry heating and hydrothermal methods: Effects on pre-osteoblast cell responses.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-08 DOI: 10.1177/08853282251333231

Ahmed Hafedh Mohammed Mohammed, Khairul Anuar Shariff, Mohamad Hafizi Abu Bakar, Ali A Salman, Aira Matsugaki, Takayoshi Nakano, Intan Nirwana, Alexander Patera Nugraha

{"title":"A novel two-step conversion from DCPD-coated β-TCP to low crystallinity β-TCP porous scaffolds via combination between dry heating and hydrothermal methods: Effects on pre-osteoblast cell responses.","authors":"Ahmed Hafedh Mohammed Mohammed, Khairul Anuar Shariff, Mohamad Hafizi Abu Bakar, Ali A Salman, Aira Matsugaki, Takayoshi Nakano, Intan Nirwana, Alexander Patera Nugraha","doi":"10.1177/08853282251333231","DOIUrl":"https://doi.org/10.1177/08853282251333231","url":null,"abstract":"This study presents a novel two-step process to fabricate low crystallinity (LC) β-tricalcium phosphate (β-TCP) porous scaffolds and evaluates their implications for pre-osteoblast cell responses. The novelty of this study lies in the two-step conversion of dicalcium phosphate dihydrate (DCPD) -coated β-TCP porous scaffold into LC β-TCP porous scaffolds through a combination of dry heating and hydrothermal conditions at 200°C. The obtained LC β-TCP porous scaffolds were characterised using a Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD), Fourier-Transform Infrared (FTIR), porosity, and compressive strength analysis confirmed the successful fabrication of LC β-TCP scaffolds. Besides, in vitro tests using pre-osteoblast MC3T3-E1 cells were conducted to investigate the cell responses toward LC β-TCP porous scaffolds. The results revealed that the LC β-TCP porous scaffolds were successfully fabricated by converting the DCPD-coated β-TCP into the dicalcium phosphate anhydrous (DCPA) coated β-TCP, followed by a hydrothermal process in a 0.1 mol/L calcium chloride (CaCl2) aqueous solution at 200°C for 24 hours to obtain LC of pure β-TCP scaffold. Moreover, in vitro cell study indicated that the cell density and proliferation surrounding the surface of the LC β-TCP porous scaffold were greater than DCPD-coated β-TCP porous scaffolds. The findings from this study are expected to significantly impact bioceramic technology by enhancing cell responses.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251333231"},"PeriodicalIF":2.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of foreign body response and systemic toxicity of additively manufactured nanocellulose reinforced alginate gelatin-based scaffolds with interconnected 3D porous structure.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-03 DOI: 10.1177/08853282251330744

Sulob Roy Chowdhury, Bikramjit Basu

{"title":"Analysis of foreign body response and systemic toxicity of additively manufactured nanocellulose reinforced alginate gelatin-based scaffolds with interconnected 3D porous structure.","authors":"Sulob Roy Chowdhury, Bikramjit Basu","doi":"10.1177/08853282251330744","DOIUrl":"https://doi.org/10.1177/08853282251330744","url":null,"abstract":"The last two decaes have witnessed significant efforts to develop gelatin/alginate based scaffolds using variants of 3D printing techniques. However, their biocompatibility for regenerating complex soft tissues remains insufficiently explored. Addressing this gap, we fabricated 3D-printed alginate-gelatin (3A5G) and nanocellulose-reinforced (3A5G1C) hydrogel scaffolds with clinically relevant dimensions (15 mm diameter, 5 mm height) and the host tissue responses were critically analyzed. The distinct advantages of nanocellulose in modulating mechanical strength, viscoelasticity, swelling, and degradation characteristics were established in our prior studies. This investigation aimed to comprehensively evaluate the foreign body response of these scaffolds in a rat model. The animals exhibited healthy metabolic activity, evidenced by progressive weight gain, localized tissue healing, and normal mobility over 30 days. Histological analyses could not reveal any adverse immune reaction at 7- or 30-days, post-implantation. Hematological and serum biochemical assessments indicated a progression from acute (7 days) to sub-acute (30 days) inflammation, following subcutaneous implantation, without any signature of systemic toxicity. Immune marker evaluation (TNF-α, CD-8, CD-68, COX-2, IL-6) confirmed the absence of pathological immune responses, even with nanocellulose incorporation. Immunohistochemical analysis using CD31 staining demonstrated enhanced vascularization in nanocellulose-reinforced scaffolds at both 7 and 30 days. The absence of systemic toxicity from scaffold degradation products and the favorable biocompatibility outcomes underline the potential of these hydrogel scaffolds for soft tissue regeneration. The incorporation of nanocellulose further enhanced the scaffolds' functional performance, particularly in promoting vascularization, positioning them as promising candidates for complex tissue engineering applications.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251330744"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-03 DOI: 10.1177/08853282251332050

Hai Yan, Fei Xia

{"title":"3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects.","authors":"Hai Yan, Fei Xia","doi":"10.1177/08853282251332050","DOIUrl":"https://doi.org/10.1177/08853282251332050","url":null,"abstract":"Three-dimensional (3D) printing has huge potential in the medical field, including bone tissue engineering scaffolds. In our study, the 3D-printed poly (lactic-co-glycolic acid) (PLGA)/nano-hydroxyapatite (HA) scaffolds carrying adipose-derived mesenchymal stem cells (ADMSCs) were constructed, and whether such scaffolds have therapeutic potential in bone defects was investigated. For in vitro assays, rat ADMSCs were implanted into blank cell wells (Blank) and PLGA/nHA and ADMSCs/PLGA/nHA scaffolds. The vitality and proliferation of ADMSCs were detected through calcein-AM/PI staining and CCK-8 assay to assess the biocompatibility of the scaffolds. ADMSCs in three groups were incubated in osteogenic induction medium, and ALP and ARS staining were performed after 7 days and 21 days, respectively. Runx2, Osterix, OCN, and OPN mRNA expression in ADMSCs was detected through RT-qPCR. For in vivo assays, rat models of radius defects were implanted by PLGA/nHA scaffolds or ADMSCs/PLGA/nHA scaffolds, and micro-CT scan analysis was conducted at week 12 after implantation. Bone marrow cell formation and Runx2 expression in rat radius tissues were evaluated through H&E and immunohistochemical staining, respectively. The results showed that ADMSCs/PLGA/nHA scaffolds provided transplanted cells with a stable carrier as well as maintained their activity and facilitated their proliferation. ADMSCs/PLGA/nHA promoted ADMSC osteogenic differentiation in vitro. Besides, the implantation of ADMSCs/PLGA/nHA scaffolds improved bone regeneration, enhanced bone marrow cell formation, and increased Runx2 expression in rat models of radius defects. Collectively, the 3D-printed ADMSCs/PLGA/nHA scaffolds effectively promote ADMSC osteogenic differentiation and exhibit significant bone repair effects, suggesting its therapeutical potential for bone defects.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251332050"},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to "Electrospun PLGA and PLGA/gelatin scaffolds for tubularized urethral replacement: Studies in vitro and in vivo". 用于管状尿道置换的电纺聚乳酸和聚乳酸/明胶支架：体外和体内研究"。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2023-12-29 DOI: 10.1177/08853282231217938

引用次数: 0

Impact of composition and surfactant-templating on mesoporous bioactive glasses structural evolution, bioactivity, and drug delivery property. 组成和表面活性剂模板对介孔生物活性玻璃结构演变、生物活性和药物传递特性的影响。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2025-01-08 DOI: 10.1177/08853282241312040

Dana Almasri, Yaser Dahman

{"title":"Impact of composition and surfactant-templating on mesoporous bioactive glasses structural evolution, bioactivity, and drug delivery property.","authors":"Dana Almasri, Yaser Dahman","doi":"10.1177/08853282241312040","DOIUrl":"10.1177/08853282241312040","url":null,"abstract":"This study explores mesoporous bioactive glasses (MBGs) that show promise as advanced therapeutic delivery platforms owing to their tailorable porous properties enabling enhanced drug loading capacity and biomimetic chemistry for localized, sustained release. This work systematically investigates the complex relationship between MBG composition and surfactant templating on structural evolution, in vitro bioactive response, resultant drug loading efficiency and release. A total of 12 samples of sol-gel-derived MBG were synthesized using cationic and non-ionic structure-directing agents (cetyltrimethylammonium bromide, Pluronic F127 and P123) while modulating the SiO2/CaO content, generating MBG with surface areas of 60-695 m2/g. Electron microscopy and nitrogen desorption studies verified the successful synthesis of the 12 ordered MBG formulations. Assessment of hydroxyapatite conversion kinetics via FTIR spectroscopy and SEM demonstrated accelerated deposition for 70-80% SiO2 formulations, independent of the surfactant used. However, the templating agent had an impact on drug loading as observed in this study where MBG synthesized by the templating agent Pluronic P123 had higher drug loading compared to the other surfactants. To determine the drug release mechanisms, the in vitro kinetic profiles were fitted to various mathematical models including ze-ro. Most compositions exhibited release properties closest to zero-order, indicating a concentration-independent drug elution rate. These results in this study explain the relationship between tailored hierarchical architecture and intrinsic ion release rates to enable advanced functionality.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1064-1083"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11877986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative buccal film delivery system for hypertension: Combining candesartan and hydrochlorothiazide.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2025-02-01 DOI: 10.1177/08853282251317853

Ola Tarawneh, Aisha Kanan, Walid Al-Zyoud, Mohammad Khanfar, Mohammad Hailat, Ala A Alhusban, Razan Abu Jaber

{"title":"Innovative buccal film delivery system for hypertension: Combining candesartan and hydrochlorothiazide.","authors":"Ola Tarawneh, Aisha Kanan, Walid Al-Zyoud, Mohammad Khanfar, Mohammad Hailat, Ala A Alhusban, Razan Abu Jaber","doi":"10.1177/08853282251317853","DOIUrl":"10.1177/08853282251317853","url":null,"abstract":"This study explores the development of buccal films as an innovative drug delivery system for hypertension management, combining candesartan cilexetil (CC) and hydrochlorothiazide (HCTZ). Utilizing a solvent casting technique, films were prepared with various polymers, including PVA, PVP, and CMC Na, and enhanced with plasticizers to improve solubility and bioavailability. The films were evaluated for physicochemical and mechanical properties, disintegration time, and drug content uniformity. Results demonstrated that the films were visually uniform, with excellent folding endurance and a neutral pH. The formulation containing PVA, PVP, and CMC Na exhibited the fastest disintegration rate and superior bioadhesion. The use of Dynamic Mechanical Thermal Analysis (DMTA) confirmed the miscibility of the drugs within the polymeric matrix, ensuring effective delivery. This novel formulation offers significant advantages by improving patient compliance and bypassing first-pass metabolism, presenting a promising alternative for hypertension treatment.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1014-1029"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction: Transplantation of copper-doped calcium polyphosphate scaffolds combined with copper (II) preconditioned bone marrow mesenchymal stem cells for bone defect repair. 撤回：移植掺铜聚磷酸钙支架与铜（II）预处理骨髓间充质干细胞用于骨缺损修复。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2024-10-24 DOI: 10.1177/08853282241284192

引用次数: 0

Comprehensive review of 3D printing techniques emphasizing thermal characterization in biomedical prototyping. 全面回顾 3D 打印技术，强调生物医学原型制作中的热特性分析。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2025-01-12 DOI: 10.1177/08853282251314672

Jayeeta Chattopadhyay, Nimmy Srivastava, Tara Sankar Pathak

引用次数: 0

Mechanobiological enhancement of electrospun PCL/nHA membranes for guided tissue regeneration applications. 静电纺丝PCL/nHA膜在引导组织再生应用中的机械生物学增强。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2025-01-05 DOI: 10.1177/08853282241312285

Niki Dadgari, Hamidreza Fotoukian, Masoumeh Haghbin Nazarpak, Mehran Solati-Hashjin

{"title":"Mechanobiological enhancement of electrospun PCL/nHA membranes for guided tissue regeneration applications.","authors":"Niki Dadgari, Hamidreza Fotoukian, Masoumeh Haghbin Nazarpak, Mehran Solati-Hashjin","doi":"10.1177/08853282241312285","DOIUrl":"10.1177/08853282241312285","url":null,"abstract":"This study aims to investigate the effects of adding nano-hydroxyapatite (nHA) to electrospun polycaprolactone (PCL) membranes for use in dental root regeneration. Porous membranes containing varying amounts of nHA (0, 1, 1.5, and 2.5 wt%) were fabricated using the electrospinning method. The physicochemical, mechanical, and biological properties of the membranes were evaluated. The synthesized nHA particles had an average size of 52 nm. Electrospun membranes exhibited uniform fibrous morphology with porosities ranging from 56% to 86%. Cyclic thermal stress (5°C-50°C) improved the mechanical properties of the composite membranes, resulting in a decrease in ultimate tensile strength (UTS) for pristine PCL from 3 ± 0.12 MPa to 1.7 ± 0.11 MPa, while the UTS for PCL membranes containing 1.5% nHA increased from 3.3 ± 0.30 MPa to 4.18 ± 0.28 MPa. In vitro bioactivity in simulated body fluid (SBF) showed enhanced apatite formation, particularly after 21 and 28 days. Cytotoxicity assays with MG-63 osteoblast-like cells demonstrated good biological performance. The incorporation of nHA not only improved the mechanical properties but also enhanced the bioactivity and cytocompatibility of the electrospun PCL membranes, making them promising candidates for guided tissue regeneration (GTR) applications.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1037-1054"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biodegradation study in FBS media of polycaprolactone patch as a potential prenatal treatment for myelomeningocele.

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-04-01 Epub Date: 2025-01-29 DOI: 10.1177/08853282251316894

K Benabdderrahmane, J Stirnemann, S Ramtani, C Falentin-Daudré

{"title":"Biodegradation study in FBS media of polycaprolactone patch as a potential prenatal treatment for myelomeningocele.","authors":"K Benabdderrahmane, J Stirnemann, S Ramtani, C Falentin-Daudré","doi":"10.1177/08853282251316894","DOIUrl":"10.1177/08853282251316894","url":null,"abstract":"Myelomeningocele (MMC) is a congenital defect of the spine characterized by meningeal and spinal cord protrusion through open vertebral archs, and its exposure to the amniotic fluid. Given that the progression of neuronal loss begins early in fetal life, an early coverage of the defect is required to improve the neurological outcomes. Several studies have proposed patches as an alternative to full surgical repair, to achieve an early protection of the spine and possibly reduce the rate of complications of current prenatal surgical procedures. In our previous work, we developed a biocompatible, watertight and biodegradable patch to improve in utero MMC repair. This patch offers an anti-adhesive internal surface to prevent adhesion to spinal cord tissue, and a bioactive external surface to promote tissue coverage. The aim of this study is to assess the patch's in vitro degradation in an amniotic-fluid-like medium and investigate the surface functionalization effect, to understand its mechanism and predict the patch's behavior over time. The study was carried out for 24 weeks in FBS medium and after each period the samples were characterized by differential scanning calorimetry, scanning electron microscopy, steric exclusion chromatography, toluidine blue assay and contact angle measurement. The results revealed a progression of PCL hydrolysis over time, characterized by a decrease in molar mass and evidence of erosion as observed by SEM. Furthermore, this process appears to be accelerated by ozonation, compared to surface functionalization without ozonation. The latter can be considered as the most suitable technique to preserve the patch structure over time, while benefiting from the grafting polymers properties during the first weeks of implantation.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1097-1114"},"PeriodicalIF":2.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0