Journal of Biomaterials Applications最新文献

{"title":"Preparation of SF-gel-CS-Hap bionic biphasic porous scaffolds and evaluation of physical, mechanical and biological properties.","authors":"Mingxi Gu, Lin Guo, Changcheng Wang, Fengde Tian, Ruihu Hao","doi":"10.1177/08853282251329591","DOIUrl":"10.1177/08853282251329591","url":null,"abstract":"<p><p><b>Objective:</b> Full-thickness cartilage defect are usually accompanied by subchondral bone damage, which is difficult to self-repair once damaged due to the lack of vascularization and innervation. In this study, a biphasic composite scaffold was developed by combining vacuum freeze-drying and iterative freeze-thawing with gelatin, chitosan, silk fibroin, and hydroxyapatite as the basic materials to explore the feasibility of using them for the repair of total cartilage defects. <b>Methods and Results:</b> Six groups of SF-CS-Gel-nHap porous scaffolds (Hap-0%, Hap-1%, Hap- 2%, Hap-3%, Hap-4%, Hap-5%) were prepared by vacuum freeze-drying and chemical cross-linking using filipin protein (SF), gelatin (Gel), chitosan (CS) and hydroxyapatite (Hap) as the base materials. A series of characterization methods were used to systematically analyze and test the morphological features as well as physical and mechanical properties of the scaffolds. Then a novel bionic biphasic porous scaffold was developed by a combination of freeze-drying and freeze-thawing using the SF-CS-Gel as the cartilage phase and the SF-CS-Gel-2%Hap as the subchondral bone phase. Finally, it was co-cultured with chondrocytes to verify the biological properties of the SF-CS-Gel/SF-CS-Gel-2%Hap bionic biphasic porous composite scaffold in vitro. The results showed that the SF-CS-Gel/SF-CS-Gel-2%Hap biphasic scaffolds had a highly porous mesh structure, with an average pore size of 156.06 ± 42.36 μm in the cartilage phase and 214.38 ± 65.82 μm in the subchondral bone phase. Co-cultured with chondrocytes, the live and dead cells stained, cck-8 growth and proliferation curves showed that the bionic scaffolds had good biocompatibility and cytotoxicity. Cytoskeletal staining showed that the morphology of chondrocytes in the bionic scaffolds could maintain three-dimensional growth as in vivo. <b>Conclusion:</b> The results showed that SF-CS-Gel/SF-CS-Gel-2%Hap biphasic scaffolds have good biocompatibility, biodegradability, stability, appropriate mechanical properties and porosity, and are suitable for repairing articular cartilage and subchondral bone. It is expected to be used as a repair material for articular cartilage in clinical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"61-81"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Bone Regeneration with Silybin-Loaded PCL/Gelatin/Nanoclay Nanocomposite Scaffolds: An In Vitro & In Vivo Study.","authors":"Mohammad Hossein Tayeed, Maryam Tehranchi, Arian Ehterami, Fereshteh Shanei, Ferial Taleghani, Hasan Semyari, Saeed Mahdipour Ganji, Nika Mehrnia, Shaghayegh Bozorgzadeh, Sepehr Zamani, Majid Salehi","doi":"10.1177/08853282251329901","DOIUrl":"10.1177/08853282251329901","url":null,"abstract":"<p><p>This study focuses on the development of a 3-dimensional porous scaffold using Polycaprolactone/Gelatin/Nanoclay (PCL/GNF/NC) for bone tissue engineering. The scaffold incorporates varying dosages of silybin (Sil) through a mixture of electrospinning and thermal-induced phase separation (TIPS) techniques. Assessments of surface shape, porosity, compressive strength, water contact angle, degradation rate, releasing profile, hemolysis, and cell proliferation were among the investigations carried out to appraise the manufactured scaffolds. In vivo evaluation utilized a rat calvaria defect model, with histological analysis employed to assess the results. The scaffolds exhibited porosity within the range of 70-90%, and those containing silybin demonstrated lower compressive strength and contact angle, along with a higher degradation rate compared to those without silybin. Release experiments revealed a 61.09% release of silybin after 28 days. In both in vivo and in vitro assessments, the PCL/GNF/NC/Sil1% scaffold displayed superior cell proliferation and bone healing properties compared to other groups. These findings suggest the potential efficacy of silybin in bone defect treatment, warranting further investigation in future research.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"105-117"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteointegration and remodeling of a novel mineral magnesium phosphate-based bone glue in-vivo.","authors":"Stefanie Hoelscher-Doht, Andrea Ewald, Andreas Fuchs, Paul Otto, Julia Bator, Jan-Peter Grunz, Karl Kneist, Lasse Bögelein, Uwe Gbureck","doi":"10.1177/08853282251326033","DOIUrl":"10.1177/08853282251326033","url":null,"abstract":"<p><p>BackgroundBone adhesives represent a major challenge in the field of materials science. Magnesium phosphate-based mineral bone cements exhibit high biocompatibility, particularly for the use as bone substitutes. In combination with calcium ions and phytic acid as liquid components, these cements demonstrate adhesive properties and potentially represent a promising new type of bone glue. Two of these first cement-adhesives were further investigated for their in vivo properties on distal femoral defects in rabbits in this study.Materials and methodsIn 4.5-month-old New Zealand White rabbits, distal lateral femoral defects in the metaphyseal region were grafted with two different magnesium phosphate-based bone adhesives (MgO, Mg2_,75Ca_0,25(PO₄)₂, phytic acid 22,5/25%wt). After 6 weeks in vivo, the adhesives were evaluated by histologic examination, biomechanical testing, imaging, XRD, and porosimetry measurements for biocompatibility and remodeling to bone.ResultsWhile the bone adhesives showed a higher density on pQCT than the surrounding cancellous bone the biomechanical testing revealed a significantly lower initial stiffness of the adhesives 6 weeks after implantation compared to both the cancellous bone and the cuboid testing before implantation. The decrease in stability was confirmed by the degradation demonstrated histologically as well as by imaging morphology. Remodeling to bone was not yet complete.ConclusionBoth bone adhesives exhibited high biocompatibility without foreign body reactions. Imaging and histology presented a homogeneous picture of degradation of both adhesives and remodeling to bone, which was not yet complete 6 weeks after the implantation.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"82-91"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous oxidized dextran sponges for surgical hemostasis and infection control.","authors":"Hong Xu, Siyuan Li, Peitao Zhuang","doi":"10.1177/08853282251329202","DOIUrl":"10.1177/08853282251329202","url":null,"abstract":"<p><p>Surgical procedures frequently result in varying degrees of bleeding and infection, which can impede patient recovery, particularly in situations of limited blood supply. Minimizing surgical blood loss and preventing infections remain crucial clinical challenges. To address these tissues, we developed a porous hemostatic sponge by aldehyde-functionalizing dextran. The high porosity and blood absorption capacity of ODex sponges enables them to effectively concentrate red blood cells, platelets, and coagulation factors, forming a blood clot together with the sponge matrix. The aldehyde groups bind to the amines in the tissue, helping to seal the bleeding site. This innovation significantly reduced clotting times in both in <i>vitro</i> and in <i>vivo</i> experiments. Furthermore, the sponge demonstrated excellent biocompatibility and potent antimicrobial activity. These findings highlight oxidized dextran as a highly promising hemostatic biomaterial with strong antimicrobial capabilities, offering the potential for broad clinical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"50-60"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing gelatin hydrogels: Synergistic effects of C-phycocyanin and nopal mucilage on physicochemical and biocompatibility properties.","authors":"Emma Gabriela Antonio-Marcos, Hugo Joel Carrillo Escalante, Liliana Hernández-Vázquez, Gerardo Alfonso Castillo Gamboa, José Manuel Cervantes Uc, Jesús Alejandro Claudio Rizo, Nayeli Rodríguez-Fuentes","doi":"10.1177/08853282251355114","DOIUrl":"10.1177/08853282251355114","url":null,"abstract":"<p><p>Healing persistent wounds is a current challenge for healthcare systems. Addressing this type of problem requires new and improved materials that activate regenerative processes without side effects. In this sense, in this study, C-phycocyanin (CPC), a bioactive pigment obtained from <i>Arthrospira platensis</i>, and nopal mucilage (MUC), a traditional Mexican element of ancestral medicine, were incorporated into gelatin (GEL)-based hydrogels and chemically crosslinked. These materials, referred to as HGEL-CPC-MUC, were prepared with varying concentrations of CPC-MUC (0-1 μg/μL of hydrogel), and their structural, physicochemical, rheological and <i>in</i> <i>vitro</i> biocompatibility properties were systematically evaluated. The main findings revealed that the incorporation of CPC-MUC into GEL-based hydrogels, significantly improves their physicochemical, mechanical and biological properties. These hydrogels exhibited a chemical crosslinking, achieving 93% crosslinking efficiency, high swelling behavior (∼1250%), rough porous surfaces, sustained degradation at physiological pH, and high thermal stability. Their rheological behavior showed an improvement in G' (226%) under thermal stress (40 °C), along with high damping capacity under constant load with the addition of CPC-MUC. Notably, the presence of CPC-MUC imparted a hemoprotective effect, with hemolysis percentages decreasing proportionally to the CPC-MUC content and none of the hydrogels interfered with coagulation pathways. Furthermore, all hydrogels demonstrated excellent <i>in</i> <i>vitro</i> biocompatibility with dermal fibroblasts, showing no cytotoxic effects. These features become important in the context of a moist and refractory wounds such as foot ulcers and extensive burns, were moisture control, exceptional hemocompatibility and support for dermal fibroblasts viability are required, as well as the porous structure for nutrients and waste exchange. HGEL-CPC-MUC hydrogels represent a highly promising biocompatible and multifunctional scaffold for advanced wound care and regenerative medicine applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251355114"},"PeriodicalIF":2.3,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylene blue functionalized ZnO nanoparticles: A promising approach for photodynamic therapy in the treatment of leishmaniasis.","authors":"Thainara Alves Gouvea, Jessica Aparecida Ribeiro Ambrósio, Janicy Arantes Carvalho, Vitor Luca Moura Marmo, Luciana Maria Cortez Marcolino, Juliana Guerra Pinto, Juliana Ferreira-Strixino, Andreza Ribeiro Simioni, Erika Peterson Gonçalves","doi":"10.1177/08853282251350317","DOIUrl":"https://doi.org/10.1177/08853282251350317","url":null,"abstract":"<p><p>Zinc oxide (ZnO) has wide application in engineering, but its use in medical sciences has aroused growing interest. In this context, ZnO nanoparticles were investigated as vehicles for the delivery of methylene blue (MB), a photosensitizer (PS) used in photodynamic therapy (PDT) against <i>Leishmania braziliensis</i>. ZnO-NPs were produced by a coprecipitation method and characterized by several techniques, including scanning electron microscopy (SEM), UV-VIS spectroscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The results showed that the ZnO-NPs presented uniform spheroidal morphology with open porosity, allowing an efficient methylene blue (MB) encapsulation without significant structural changes, ensuring stability and the absence of aggregation. The PS was adsorbed on the porous surface of the ZnO nanoparticles, characterized by scanning electron microscopy (SEM) and steady-state analysis techniques. Spectroscopic analysis confirmed the maintenance of the photosensitizing properties of MB. The biological activity was evaluated in vitro using the trypan blue exclusion method in macrophages infected with <i>Leishmania braziliensis</i>. After loading with the photosensitizer, they maintained their photophysical properties, ensuring the proper location of the dye within the cells. In vitro assays demonstrated the internalization of ZnO/MB-NPs by infected macrophages and a significant reduction in parasite viability after light activation. Thus, the results showed that the developed system exhibits a promising photodynamic activity with relevant therapeutic potential in treating macrophages infected by <i>Leishmania braziliensis</i>.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251350317"},"PeriodicalIF":2.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an enhanced liver scaffold recellularization using fibronectin.","authors":"Sadia Afrin, Usha Yadav, Chandra J Yadav, Jihad Kamel, Jun-Young Lee, Kyung-Mee Park","doi":"10.1177/08853282251350315","DOIUrl":"https://doi.org/10.1177/08853282251350315","url":null,"abstract":"<p><p>Decellularized liver scaffolds offer a promising foundation for liver tissue engineering and regenerative medicine. However, several challenges such as poor cell adhesion, inefficient reseeding, inadequate vascularization, and a high risk of blood clot formation continue to hinder their clinical application. While fibronectin (FN) has been widely used to enhance scaffold functionality, its potential for liver-specific applications remains largely unexplored. In this study, we developed a perfusion-assisted FN coating technique to improve the adhesion of endothelial cells (EA.hy926) and hepatocytes (HepG2), thereby enhancing the overall biocompatibility of liver scaffolds. FN was carefully introduced into decellularized rat liver scaffolds, allowing for targeted deposition across both the vascular and parenchymal compartments to optimize cellular attachment. Following portal vein reseeding and 7 days of bioreactor incubation, the FN-coated scaffolds showed significantly better endothelial cell adhesion within blood vessel structures and increased HepG2 cell coverage throughout the liver tissue. Immunohistochemistry (IHC) confirmed enhanced HepG2 proliferation, while TUNEL and RT-qPCR analyses indicated improved cell viability and scaffold functionality. Additionally, ex vivo blood perfusion tests demonstrated reduced thrombogenicity, likely due to improved endothelialization and lower platelet adhesion. These findings highlight FN functionalization as an effective bioengineering approach to overcoming key barriers in vascularization, biocompatibility, and cellular integration for liver scaffolds. By extending the known benefits of FN beyond its previously studied applications in kidney and heart scaffolds, this research introduces a promising strategy for advancing bioengineered liver grafts and potential transplantation models.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251350315"},"PeriodicalIF":2.3,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144234203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and antibacterial alginate/lignin composite hydrogels loaded with vancomycin for wound dressing applications.","authors":"Faeze Shojaeinia, Masoumeh Haghbin Nazarpak, Akbar Karkhaneh","doi":"10.1177/08853282251347768","DOIUrl":"https://doi.org/10.1177/08853282251347768","url":null,"abstract":"<p><p>Hydrogels are advantageous for wound healing as they provide mechanical support and maintain a moist environment, essential for tissue repair. Although conventional alginate-based hydrogels are commonly used in wound care, they often lack essential properties like antibacterial and antioxidant functionality. To address this limitation, this research focused on synthesizing composite hydrogels combining alginate with lignin and loading them with Vancomycin. The incorporation of lignin and Vancomycin imparted antibacterial and antioxidant properties to the hydrogels, enhancing their therapeutic potential. The hydrogels are dual crosslinked (physically and chemically), where lignin counteracts high levels of reactive oxygen species and reduces excessive inflammation at the wound site. Furthermore, the hydrogels had pores ranging from 100 to 135 μm, which is beneficial to gas and nutrient exchange and wound fluid absorption. Results showed that lignin improved the hydrogels' stability in physiological conditions by 50%. Additionally, the incorporation of lignin led to a 30% increase in antioxidant activity and a 50% boost in antibacterial activity. Vancomycin release from the hydrogels was measured, which showed alginate-only hydrogels releasing 50% and lignin-reinforced hydrogels releasing 35% over the first 24 hours. The MTT test confirmed approximately 90% cell viability across all samples, suggesting that the designed hydrogels are promising candidates for wound dressing applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251347768"},"PeriodicalIF":2.3,"publicationDate":"2025-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-hydroxyapatite doped tyramine gelatin/silk fibroin scaffold for the regeneration of cancellous bone defects.","authors":"Lina Yang, Mengting Wang, Caixing Peng, Xiang Gong, Lihong Fan, Shuhua Liu, Shengxiang Tao","doi":"10.1177/08853282251347346","DOIUrl":"https://doi.org/10.1177/08853282251347346","url":null,"abstract":"<p><p>Gelatin (G) and silk fibroin (SF) are well-established as scaffold materials for bone regeneration; however, their limited binding abilities and mechanical properties often result in less-than-ideal outcomes. In this study, we sought to enhance the stability of a silk fibroin/gelatin biomimetic scaffold by introducing a tyramine bond to the gelatin and incorporating nanohydroxyapatite as a bioactive element. This innovation led to the development of a more robust silk fibroin/nano-hydroxyapatite/gelatin tyramine biomimetic scaffold (SHGT). The biomimetic scaffold was fabricated through an enzymatic reaction catalyzed by horseradish peroxidase/hydrogen peroxide (HRP/H₂O₂), which facilitated the interaction between a high concentration of silk fibroin (17%) and gelatin tyramine (GT). Additionally, nano-hydroxyapatite (nHA) was incorporated as a bioactive filler to promote bone repair. Our findings indicated that the SHG biomimetic scaffold, initially designed as a sponge, was transformed into an SHGT scaffold with improved brittle fracture resistance, thus broadening its potential applications in bone reconstruction. Moreover, the data showed that combining GT with RGD sequences and HA as a bioactive component significantly enhanced the viability of bone marrow stromal cells (BMSCs) cultured on the scaffold. This synergistic effect highlights the potential of the SHGT scaffold as a promising material for bone tissue engineering.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251347346"},"PeriodicalIF":2.3,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oyster shell powder reinforced chitosan-poly(vinyl alcohol) freeze-dried composite sponge for on-site hemorrhage control.","authors":"Pranabesh Kumar Sasmal, Sujankrishna Samanta, Shalini Dasgupta, Samit Kumar Nandi, Abhijit Chanda, Pallab Datta","doi":"10.1177/08853282251347348","DOIUrl":"https://doi.org/10.1177/08853282251347348","url":null,"abstract":"<p><p>A composite hemostatic sponge consisting of chitosan (CS) with oyster shell powder (OSP) has been developed as a potentially sustainable composite material for controlling hemorrhage at the injury site. The system is designed assuming that Ca⁺ released by OSP will accelerate the effect of chitosan at damage sites, enhancing the overall hemostatic efficacy. The sponge was thoroughly characterized using FTIR, SEM, and EDX analysis. In vitro, blood clotting assays such as clotting time (CT) [188 ± 4 s], prothrombin time (PT) [36 ± 1 s], activated partial thromboplastin time (aPTT) [51 ± 2 s], and plasma recalcification time (PRT) [58 ± 3 s] demonstrated that the inclusion of CaCO₃ significantly improved clot formation, with the CS-OSP sponge outperforming control sponges without OSP. RT-PCR analysis of vascular endothelial growth factor A (VEGF-A), platelet-derived growth factor (PDGF), and interleukin growth factor 1 (IGF-1) on fibroblast cell lines evidenced the wound healing-promoting activity of OSP-reinforced CS sponges. This was further supported by in vivo studies using a rabbit femoral artery injury model, where the CaCO₃-enhanced sponge achieved superior hemostasis and reduced blood loss more effectively than the control sponges without CaCO₃. These findings suggest that the oyster shell-derived CaCO₃ enhances the hemostatic activity of chitosan-based sponges, providing a promising candidate for rapid hemorrhage control in clinical settings, particularly in scenarios involving both oozing and pressurized bleeding.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251347348"},"PeriodicalIF":2.3,"publicationDate":"2025-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}