Journal of Biomaterials Applications最新文献

{"title":"Corneal bioengineering via <i>electrospun nanofibers</i> and <i>nanoparticles</i>.","authors":"Majid Salehi, Zohreh Arabpour, Sepehr Zamani, Morteza Alizadeh, Maliheh Gharibshahiyan, Milad Rezvani, Niloofar Aldaghi, Seyed Meysam Yekesadat, Ali R Djalilian","doi":"10.1177/08853282251393784","DOIUrl":"10.1177/08853282251393784","url":null,"abstract":"<p><p>Nanotechnology is transforming the area of corneal tissue engineering by improving scaffold design and enabling sophisticated therapeutic strategies. Nanomaterials are being used to improve the corneal scaffolds' mechanical strength, permeability, and transparency, as well as to enable the therapeutic agents' targeted delivery by nanocarriers. These improvements deal with important problems in corneal repair, like inflammation, infections, and neovascularization. While corneal transplantation remains a standard treatment, the risk of rejection and availability of donor tissue are the main limitations. Recent improvements in electrospinning have made it possible to make nanofibers that look like the natural extracellular matrix (ECM). These fibers have a large surface area and high porosity, which help cells grow, stick to each other, and change into different types of cells. Both synthetic and natural polymers have been successfully employed to fabricate biocompatible and biodegradable nanofibers, indicating their potential for the treatment of various corneal disorders. Electrospun nanofibers are very useful for corneal tissue engineering because they are easy to use, can be used in surgery, and are structurally similar to the cornea. Adding nanofibers and nanoparticles to corneal tissue engineering improves the scaffold and allows for targeted therapies, which means that there are more advanced ways to reconstruct and rehabilitate the cornea. This study investigates the application of naturally derived and synthetic nanoparticles in drug delivery systems and the development of composite nanoparticles, highlighting their potential to improve corneal tissue engineering techniques.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1094-1140"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145409147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structurally engineered silver-albumin nanocomposites functionalized with alpha-terpinyl acetate for enhanced biocompatibility and anticancer activity.","authors":"Sreejith Thrivikraman Nair, Kaladhar Kamalasanan, Althaf Umar Kp, Sruthy Sunil, Sona Thankachan, Sunil Kumar, B Anagha, Harika Sapa, Shona Sara Shaji, Sherin Ann Mathew, Ayana Kp, Bijo Mathew","doi":"10.1177/08853282251391886","DOIUrl":"10.1177/08853282251391886","url":null,"abstract":"<p><p>The rational design of biofunctional nanocomposites through structural and interfacial engineering is central to advancing next-generation biomaterials. In this study, we developed a multifunctional silver-based nanocomposite with dual-level modification; albumin (Alb) is used as a biopolymeric stabilizer, while <i>Elettaria cardamomum</i> extract, rich in alpha-terpinyl acetate (aTA), served as a surface-functionalizing agent. Gas chromatography-mass spectrometry (GC-MS) confirmed aTA as the predominant phytoconstituent (97.7% match). Dynamic light scattering revealed progressive size increases from 67.17 nm (AgNPs) to 145.73 nm (Alb-AgNPs) and 365.7 nm (Alb-AgNPs-aTA), indicating successful stepwise functionalization. Structural transformations were supported by UV-Vis spectroscopy and X-ray diffraction (XRD), which revealed changes in surface plasmon resonance and crystalline phases. Thermal analysis (DSC and TGA) demonstrated improved thermal stability, with a pronounced DTG peak at 333.2°C. Molecular dynamics simulations suggested strong Alb-aTA interactions that enhance nanocomposite stability. <i>In vitro</i> assays on HCT-116 colorectal cancer cells showed improved biocompatibility and anticancer efficacy for Alb-AgNPs-aTA (IC₅₀ = 24 µg/mL). This study presents a thermally stable, structurally engineered nanocomposite with demonstrated bioactivity and potential applicability in drug delivery and cancer therapy, contributing to the broader understanding of how nanoscale modifications influence biological performance.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1165-1182"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic enhancement of bone regeneration by obacunone-loaded peg-based composite scaffolds.","authors":"Xiuchen Yuan, Fengpin Yao, Dongfeng Li, Cunxiao Li, Lei Meng, Ye Zhang, Hao Wang, Yan Dong, Jun Shang","doi":"10.1177/08853282251390644","DOIUrl":"10.1177/08853282251390644","url":null,"abstract":"<p><p>In this study, we report the design and fabrication of a novel biomimetic composite scaffold (PSGO) and systematically assess its potential for bone tissue engineering. The PSGO scaffold was fabricated using three-dimensional (3D) printing technology with a base matrix composed of polyethylene glycol (PEG), sodium alginate (SA), and gelatin (GEL). Obacunone-loaded polycaprolactone (OA@PM) microspheres were embedded within the scaffold to enable sustained drug release, thereby creating a structure with precise architecture and functional gradients. Comprehensive characterization of the scaffold's surface morphology, rheological properties, and drug release behavior was performed. In vitro experiments demonstrated that the PSGO scaffold significantly promoted the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs), enhanced the expression of key osteogenic markers (RUNX-2 and OCN), and facilitated mineralized matrix formation. Furthermore, in vivo evaluation using a rat calvarial critical-size defect model-assessed via micro-computed tomography and histological analysis-confirmed its excellent osteogenic performance, with substantial new bone formation observed at both the defect margins and center. With its outstanding biocompatibility, osteoinductive capabilities, and controlled drug release properties, the PSGO scaffold offers a promising new approach for the clinical repair of large-scale bone defects.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1153-1164"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145354930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mycosynthesised iron oxide nanoparticles using <i>Apiospora aurea</i>: Mitigating somatic and germline toxicity for safer biomedical applications.","authors":"Asha Priya Mandarada, Vamshi Krishna Eruva, Nikhila Yaladanda, Sunil Misra, Srinivasa Rao Mutheneni","doi":"10.1177/08853282251393788","DOIUrl":"10.1177/08853282251393788","url":null,"abstract":"<p><p>Iron oxide nanoparticles (FeONPs) have promising biomedical applications but are limited by potential cytotoxic and genotoxic risks. This study addresses these concerns by synthesizing mycosynthesized FeONPs (M.FeONPs) having angiogenic properties using <i>Apiospora aurea</i>, a mangrove-derived fungus, to enhance biocompatibility and reduce toxicity. The results showed that chemically synthesized FeONPs induced oxidative stress, cell cycle arrest, and apoptosis, whereas M.FeONPs exhibited lower toxicity and better compatibility in CHO-K1 cells. In vitro, genotoxicity assessments further revealed that FeONPs caused significant chromosomal aberrations and DNA damage, while M.FeONPs had reduced genotoxic effects. In vivo studies using Swiss albino mice confirmed that M.FeONPs induced minimal systemic toxicity, maintaining stable hematological and biochemical profiles, unlike FeONPs, which triggered immune stress and mild organ inflammation. In vivo, genotoxicity studies also demonstrated that M.FeONPs caused lesser clastogenic, mitotic, aneugenic, and teratogenic effects than chemically synthesized FeONPs. Hence, these findings confirm the potential of M.FeONPs for biomedical applications, particularly in reproductive health and therapeutics applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1198-1217"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145409224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human blood preconditioned porcine arteries as potential conduits for human transplantation: Proof of concept in rabbit.","authors":"Vijay Kumar Kuna, Bo Xu, Niclas Kvarnström, Suchitra Sumitran","doi":"10.1177/08853282251391312","DOIUrl":"10.1177/08853282251391312","url":null,"abstract":"<p><p>Tissue-engineered arteries using natural scaffolds could overcome the drawbacks of autografts or artificial conduits used in the repair of many congenital cardiac defects and coronary artery bypass grafts. In this study, we present a novel approach based on the use of decellularized xenogeneic matrix scaffolds preconditioned with human peripheral blood stem cells for future cardiovascular therapy. Cellular components of porcine carotid arteries (n = 40) were removed with physical, chemical and enzymatic means. The decellularized arteries were preconditioned by perfusion with human peripheral blood solution for 10 days. The decellularized and preconditioned grafts were characterized for their histological and functional integrity. To demonstrate proof-of-concept, we used a sub-acute (96 h) rabbit model where either only decellularized porcine arteries or preconditioned with autologous rabbit blood solution were implanted in the abdominal aorta of the animals. The rabbits were examined by Doppler ultrasound and histology. Histology and molecular analysis showed absence of cells and preservation of extracellular cell matrix (ECM) proteins in decellularized porcine arteries. Preconditioning of arteries with human blood showed a thin lining of intima with blood and cells. In the rabbit implant model, although blood flow was detected in all rabbits at 24 h, the animals implanted with only decellularized arteries showed lumen filled with thrombus. However, in preconditioned arteries, thrombosis was not seen at either 24 or 96 h. Taken together, these results suggest that these decellularization and preconditioning protocols using autologous blood may be adaptable for successful tissue-engineering of xeno-arteries for human application. However, further research to improve preconditioning efficiency and long-term animal studies are needed.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1218-1231"},"PeriodicalIF":2.5,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145368071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of cartilage injury using a novel plug-type scaffold immersed in hyaluronic acid.","authors":"Chuji Hirota, Kuniaki Ikeda, Takashi Ishitani, Takeru Iwata, Eichi Ito, Shunsuke Sezaki, Daisuke Morita, Yoshinori Okamoto, Hitoshi Wakama, Shuhei Otsuki","doi":"10.1177/08853282261440933","DOIUrl":"https://doi.org/10.1177/08853282261440933","url":null,"abstract":"<p><p>The osteochondral autograft transfer system has demonstrated potential for hyaline cartilage restoration; however, donor-site morbidity remains a considerable challenge. Therefore, we aimed to develop an innovative plug-type cartilage scaffold using polyglycolic acid (PGA) and poly (L-lactide-co-ε-caprolactone) [P (LA/CL)], and evaluate its effectiveness in a cartilage injury model. We fabricated cartilage plugs by wrapping PGA felt with P (LA/CL) sutures combined with a P (LA/CL) sponge, and implanted the plugs into porcine knee cartilage defects to investigate their effectiveness with and without hyaluronic acid (HA). In addition to HA infiltration and release kinetics plug assessments, we performed a histological assessment of cartilage repair. The cartilage plug scaffold exhibited drug delivery capabilities, achieving 150% HA infiltration within 30 min and sustained HA release effects for over 24 h. The cartilage plugs immersed in HA exhibited a smooth surface and rich glycosaminoglycan cartilage regeneration, indicating that HA in the scaffold may enhance chondrocyte migration and the extracellular matrix. The PGA-based cartilage plug scaffold reinforced with P (LA/CL) and immersed in HA facilitated effective hyaline cartilage repair, providing a new therapeutic option for less invasive cartilage treatment.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261440933"},"PeriodicalIF":2.5,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and performance study of strontium-carboxymethyl chitosan/silk fibroin/gelatin composite cartilage scaffolds.","authors":"Changcheng Wang, Mingxi Gu, Tianyi Song, Xiaochen Wang, Lin Guo, Fengde Tian, Bo Liu, Dewei Zhao","doi":"10.1177/08853282261436162","DOIUrl":"https://doi.org/10.1177/08853282261436162","url":null,"abstract":"<p><strong>Objective: </strong>Articular cartilage exhibits extremely poor self-repair capacity, often leading to osteoarthritis. This study aims to develop a strontium-containing porous composite cartilage scaffold (CMCS-Sr-Gel-SF) that combines bioactivity with mechanical support for cartilage injury repair.</p><p><strong>Methods: </strong>First, the carboxymethyl chitosan strontium (CMCS-Sr) composite was synthesized via a solution reaction method and characterized using EDS, XRD, and FTIR. Subsequently, pure scaffolds were prepared using CMCS-Sr at different concentrations, and their microstructure, mechanical properties, and cytotoxicity were evaluated via scanning electron microscopy (SEM), universal testing machine, and MTT assay. Based on this, CMCS-Sr at the optimal bioactive concentration (300 μg/mL) was selected and combined with silk fibroin (SF) and gelatin (Gel) to construct four three-dimensional porous scaffolds using vacuum freeze-drying technology. These scaffolds underwent a series of physicochemical characterizations, including SEM, XRD, FTIR, swelling rate, porosity, in vitro degradation rate, and mechanical properties. Cell proliferation during co-culture with chondrocytes was assessed using the CCK-8 assay to evaluate their biocompatibility.</p><p><strong>Results: </strong>Characterization results confirm that strontium ions successfully coordinate with CMCS to form stable CMCS-Sr complexes. While the pure CMCS-Sr scaffold exhibits excellent porosity (average pore size 167 ± 43.58 μm) and chondrocyte proliferation-promoting activity, its mechanical strength (compressive modulus of 185.82 kPa at 9% concentration) falls significantly short of natural cartilage requirements. The composite scaffold CMCS-Sr-Gel-SF demonstrated balanced comprehensive properties: an appropriate three-dimensional porous structure (pore size 167 ± 53.68 μm, porosity 81.62 ± 3.65%), excellent water absorption and swelling rate (2007 ± 157.3%), controllable degradation rate (53.33 ± 4.16% degradation at 28 days), and significantly enhanced compressive modulus (approximately 0.63 MPa). In vitro cell experiments demonstrate that the CMCS-Sr-Gel-SF scaffold effectively supports chondrocyte adhesion and proliferation, with cells maintaining vigorous growth activity throughout the 10-days culture period.</p><p><strong>Conclusion: </strong>The CMCS-Sr-Gel-SF scaffold successfully mimics the structure of natural extracellular matrix, enabling sustained release of strontium ions while creating a microenvironment that promotes cartilage tissue regeneration.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261436162"},"PeriodicalIF":2.5,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147574021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-clinical safety of novel cross-linked chondroitin sulfate (SI-449) as an anti-adhesion barrier for abdominal and pelvic surgeries.","authors":"Ayako Shiraki, Shino Ito, Dai Muramatsu, Tomoki Sasaki, Akira Otsuka, Tomomi Miyahara, Katsuya Takahashi, Keiji Yoshioka, Miki Suehiro","doi":"10.1177/08853282261432825","DOIUrl":"https://doi.org/10.1177/08853282261432825","url":null,"abstract":"<p><p>Postoperative adhesions result from abnormal tissue repair and most frequently occur in patients following abdominal and pelvic surgeries. Prevention of adhesion formation, including the use of anti-adhesion barriers during surgery, is considered the most effective clinical strategy for postoperative adhesions. However, no commercial product is commonly used worldwide because of its insufficient efficacy, complexity, and difficulty of use. We developed a new anti-adhesion barrier using novel biomaterial (SI-449), a cross-linked chondroitin sulfate, in an easy-to-apply powder form. Our previous study using animal models showed that the anti-adhesion efficacy of SI-449 was superior to that of an existing product. In this study, the safety of SI-449 was evaluated based on the results of systemic toxicity tests in rats after intraperitoneal application and a wound healing test in laparotomized rats to mimic its clinical use in abdominal and pelvic surgeries. In the systemic toxicity test, minimal and transient hematological changes and muscular regeneration in the abdominal wall and ileum were observed and were considered to be related to systemic toxicity or local irritation due to SI-449. However, these findings were within the safety margin and not associated with serious toxicity. Other changes were considered physiological reactions to SI-449 or its degradants. In the wound healing test, SI-449 was found to have no effect on the healing of the incisional abdominal wall. In conclusion, the non-clinical safety of SI-449 was confirmed, and this novel biomaterial is expected to be used as an anti-adhesion barrier in abdominal and pelvic surgeries.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261432825"},"PeriodicalIF":2.5,"publicationDate":"2026-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147520938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization and hemocompatibility of poly (N-acryloyl-L-tryptophan) nanoparticles as targeting delivery carriers for vinblastine.","authors":"Zishan Zhou, Hongquan Tao, Haocheng Yang, Ao Duan, Jiahui Yu, Yixin Chen, Yongyan Zhu, Quanhong Zhu","doi":"10.1177/08853282261422124","DOIUrl":"https://doi.org/10.1177/08853282261422124","url":null,"abstract":"<p><p>Favorable biocompatibility is essential to biomaterials, and natural amino acids are recognized as the promising building block of polymers due to their non-toxicity and tunable side chains. We prepared polymeric nanoparticles (NPs) using N-acryloyl-L-tryptophan monomer by precipitation polymerization, and modified with polyethylene glycol and folate (PEG-FA) to improve the solubility and target folate-receptors (FR) overexpressed tumor tissues. Serving as drug carriers for vinblastine (VBL), NPs-PEG-FA with about 212.4 nm had the drug loading of VBL of 6.65 ± 0.41% after co-incubating for 1 h and showed sustained-release in pH 7.4 PBS, in which 99.87 ± 1.00% of VBL was released from NPs-PEG-FA during 72 h. Furthermore, NPs-PEG-FA was more efficiently taken up by FR positive Hela cells compared with NPs-PEG, which signified folate could enhance the internalization of NPs-PEG-FA into FR over-expressed cells. And NPs-PEG-FA began to enter Hela cells in large quantities from 3 h onwards, meanwhile the released drug increased more quickly in the first 3 h, which indicated most of the drugs would be released after entering tumor cells. More importantly, NPs-PEG-FA had good biocompatibility to L929 mouse fibroblast cells and exhibited hemocompatibility via the assays of hemolysis, antithrombogenicity, coagulation activation and platelet activation. NPs-PEG-FA could serve as drug carriers for delivering drugs into FR positive tumor cells.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261422124"},"PeriodicalIF":2.5,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147498879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and in vivo evaluation of a self-polymerizable tranilast-loaded silicone gel for scar modulation.","authors":"Farjam Goudarzi, Daniel Elieh-Ali-Komi, Sara Derakhshan, Khodabakhsh Rashidi, Amir Kiani","doi":"10.1177/08853282261432810","DOIUrl":"https://doi.org/10.1177/08853282261432810","url":null,"abstract":"<p><p>Hypertrophic scars and keloids represent pathological outcomes of wound healing characterized by excessive collagen deposition and persistent myofibroblast activity, necessitating effective therapeutic intervention. In this study, a self-polymerizable one-component silicone gel loaded with tranilast was developed and evaluated in a rabbit ear hypertrophic scar model. The formulation was characterized for physicochemical, rheological, and film-forming properties, and its biocompatibility was assessed in accordance with ISO 10993 standards. Hypertrophic scars were induced by punch biopsy on rabbit ears (n = 6), followed by topical treatment with silicone gel alone or tranilast-loaded silicone gel for 40 days. Scar remodeling was evaluated using histological staining (H&E, Masson's trichrome), α-SMA immunohistochemistry, and biochemical quantification of hydroxyproline and glycosaminoglycans. The gel rapidly polymerized on the skin to form a stable solid film and demonstrated favorable biocompatibility. Tranilast release reached approximately 81% within 24 h, following diffusion-controlled kinetics. Histological analyses revealed reduced epidermal and dermal thickness in treated groups compared to control, with the tranilast-loaded gel showing collagen fiber reorganization closely resembling healthy skin. Hydroxyproline content was significantly reduced in the tranilast group compared to both control and silicone-only groups (p < 0.01), accompanied by decreased α-SMA expression (27-30%), indicating suppression of myofibroblast activity. These findings demonstrate that the self-polymerizable silicone gel provides an effective delivery platform for tranilast, offering synergistic benefits in scar modulation and supporting its potential for advanced topical scar management.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261432810"},"PeriodicalIF":2.5,"publicationDate":"2026-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147457659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}