Journal of Biomaterials Applications最新文献

{"title":"AgNP-chitosan hydrogel as a novel antibacterial and wound healing material against methicillin-resistant <i>Staphylococcus aureus</i>.","authors":"Bhumika Jena, Gausal A Khan, Pallabi Punyatoya Sahoo, Swati Sucharita Singh, Sankha Chakrabortty, Susanta Kumar Behera, Suraj K Tripathy, Amrita Mishra","doi":"10.1177/08853282261416075","DOIUrl":"10.1177/08853282261416075","url":null,"abstract":"<p><p>Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), which is resistant to many of the antibiotics used in clinical settings, has emerged as a significant concern in healthcare and the treatment options for MRSA infections are becoming increasingly limited. There is an urgent need for novel systems to combating MRSA. Nanotechnology inspired interventions might possibly overcome the defense mechanisms used by MRSA, resulting in more successful treatment techniques. A unique strategy involved the fabrication of Ag nanoparticles (NPs) derived from <i>Urginea indica</i> and combined with chitosan. The resulting Ag-chitosan hydrogel was assessed using UV and FTIR spectroscopy, as well as zeta potential measurement. The hydrogel's efficacy against targeted bacteria and biofilms was investigated, revealing its method of action. Furthermore, the biological compatibility of the material with cell lines was analyzed for potential uses. These studies were supplemented by <i>in vitro</i> infection trials and <i>in vivo</i> assessments utilizing a Balb/c mouse model. Overall, the comprehensive analysis confirmed the Ag-chitosan hydrogel's ability to promote wound healing. Notably, adding U. indica-derived Ag NPs and chitosan significantly increased the hydrogel's therapeutic potential.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"243-258"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Material properties and antibacterial activity of carbonate apatite cement prepared with silver-containing vaterite powder.","authors":"Hirogo Minamisawa, Noboru Kajimoto, Michito Maruta, Taira Sato, Kanji Tsuru","doi":"10.1177/08853282261422903","DOIUrl":"10.1177/08853282261422903","url":null,"abstract":"<p><p>In this study, silver-containing carbonate apatite (CO₃Ap) cement was prepared by mixing silver-containing vaterite and calcium hydrogen phosphate (DCPA) powder in an aqueous phosphate solution. The material properties and antibacterial performance of the cement were evaluated. Silver ions were introduced during vaterite synthesis, resulting in a composite CO₃Ap cement containing silver phosphate (Ag₃PO₄). The addition of silver nitrate did not affect key physical properties of the cement, including strength and porosity. Antibacterial testing using inhibition zone measurements confirmed that effective antibacterial activity was achieved even at low silver contents. At high silver concentrations, the coexistence of amorphous nanoclusters and Ag₃PO₄ is expected to form a biphasic release system that enables both immediate and sustained silver ion release. These results demonstrate that introducing silver ions during vaterite synthesis is an effective approach for producing antibacterial CO₃Ap cement with strong potential for orthopedic and dental applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"233-242"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146113195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Challenges and prospects of polymeric biomaterials in immune engineering: A review.","authors":"Boyka Andonova Lilova, Desislav Dinev, Probal Basu","doi":"10.1177/08853282261418177","DOIUrl":"10.1177/08853282261418177","url":null,"abstract":"<p><p>Immune engineering is a vast and rapidly developing field with a strong focus on polymeric materials. The importance of these biomaterials in immune engineering revolves around their ability to provide bioactive molecules to the target site, which influences the immune system. Their functional performance depends on various structural and functional properties of the biomaterial, which are also influenced by the host immune microenvironment. This work focuses on the current state of the art, the attributes of polymer-based biomaterials, and their limitations in immune engineering applications. This review not only elaborates on the advantages of polymeric biomaterials in immune engineering but also critically analyses the potential of these biomaterials in this field. The current work begins with the identification of key characteristics of polymer-based biomaterials for immune engineering, then explores different aspects of various polymeric materials and their importance in immune engineering applications. One of the key advantages of polymeric materials is that they can be efficiently designed to deliver bioactive molecules that significantly influence the host immune system. On the other hand, a notable limitation of these materials involves the development of adverse immune responses that can often occur due to the incompatibility of polymeric biomaterials with the host immune system. Finally, the review delves into the future perspectives and potential of these materials in immune engineering based personalized medicine and/or engineering living material (ELM) specific applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"161-177"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13320338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional PEEK implants: Sulfonation-polydopamine synergy for bioactivity, wear resistance, and antibacterial efficacy.","authors":"Qianqian Sun, Guangru Zhang, Guofa Zhang, Mei Lv, Litao Wang","doi":"10.1177/08853282251389693","DOIUrl":"10.1177/08853282251389693","url":null,"abstract":"<p><p>PEEK is a promising biomaterial for orthopedic and dental applications due to its excellent mechanical properties, biocompatibility, and bone-like elastic modulus. However, its bioinert surface limits osseointegration and predisposes it to wear debris-induced inflammation, hindering its use in load-bearing implants. To address these challenges, this study proposes a composite modification strategy combining gradient sulfonation with polydopamine (PDA) coating to enhance the bioactivity, tribological performance, and interfacial stability of PEEK. Surface characterization revealed that sulfonation introduced porous structures and hydrophilic sulfonic acid groups, while PDA further improved wettability and enabled chelation-mediated hydroxyapatite (HA) mineralization. Tribological tests demonstrated that optimal sulfonation reduced the friction coefficient and wear width, whereas excessive sulfonation (60 min) degraded mechanical properties due to adhesive wear. In vitro mineralization confirmed that PDA-coated samples exhibited robust HA deposition, attributed to catechol/amino group-mediated nucleation. Additionally, H₂SO₄/PDA synergistically enhanced antibacterial efficacy by chemically disrupting bacterial membranes. A polyvinyl alcohol (PVA) graft layer was constructed on the surface of PEEK substrate, and its interfacial bonding performance under frictional shear load was evaluated. These results demonstrate that the H₂SO₄/PDA composite modification optimizes PEEK's multifunctional performance, offering a viable route for developing advanced biomimetic joint implants with improved osseointegration, wear resistance, and long-term stability.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"190-200"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145367770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and evaluation of a fish skin-derived acellular dermal matrix for diabetic wound healing: <i>In vitro</i> and <i>in vivo</i> assessment.","authors":"Mekha Grace Varghese, Thomas George Valliaveettil, Binsi Puthanpurakkalkizhakkethil Kamalamma, Nibu Varghese, Yogesh Bharat Dalvi, Nebu George Thomas, Rajasree Anitha Rajeevan, Anoop Chandran, Sukumaran Anil","doi":"10.1177/08853282261422121","DOIUrl":"10.1177/08853282261422121","url":null,"abstract":"<p><p><b>Introduction:</b> Non-healing diabetic foot ulcers often result in amputation and reduced quality of life. The study objective was to develop and evaluate a novel fish skin-derived acellular dermal matrix (FSADM) for diabetic wound-healing applications, addressing these challenges. <b>Methods:</b> FSADM was fabricated from yellow fin tuna (<i>Thunnus albacares</i>) skin using a decellularization and lyophilization process. The matrix was characterized by Fourier-transform infrared spectroscopy (FTIR). <i>In vitro</i> biocompatibility was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays with L929 fibroblasts and hemocompatibility tests. <i>In vivo</i>, biocompatibility was evaluated through subcutaneous implantation in Sprague-Dawley rats. Wound-healing efficacy was assessed in a streptozotocin-induced diabetic rat model with full-thickness excisional wounds, comparing FSADM with commercial alternatives and untreated controls. <b>Results:</b> FTIR analysis confirmed the preservation of collagen structure in FSADM. <i>In vitro</i> studies demonstrated cytocompatibility with L929 cells and minimal haemolytic activity (0.68 ± 0.034%). Subcutaneous implantation demonstrated good biocompatibility, with a progressive reduction in the inflammatory response. In the diabetic wound model, FSADM-treated wounds exhibited significantly faster closure rates than commercial controls (<i>p</i> < 0.05), achieving 100% closure by day 21, compared to 90% closure in commercial controls. Histological analysis revealed enhanced epithelialization, hair follicle formation, and angiogenesis in FSADM-treated wounds. <b>Conclusion:</b> FSADM demonstrates excellent biocompatibility and superior wound-healing in diabetic conditions compared to commercial alternatives. It presents a promising, sustainable biomaterial for diabetic wound care. Further studies are needed to validate these findings in clinical settings and optimize their therapeutic potential.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"221-232"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of a three-dimensional retinal-like structure using a laminin-111-modified alginate layer.","authors":"Samaneh Najafian, Mohammad Hossein Nasr Esfahani, Amirreza Haghighatnejad, Fereshteh Karamali","doi":"10.1177/08853282251414276","DOIUrl":"10.1177/08853282251414276","url":null,"abstract":"<p><p>AimOver the past few decades, the prevalence of visual impairments has increased substantially, and some are incurable. This study aimed to present a co-culture method that promotes the differentiation of retinal progenitor cells (RPCs) in the presence of retinal pigment epithelium (RPE) cells.MethodsWe implemented a three-dimensional culture system using a modified alginate substrate to culture human embryonic stem cell-derived RPCs on top of an RPE layer, mimicking an in vivo environment. The characteristics of RPCs in experimental and control groups were analyzed at the RNA and protein levels, and the 3D retinal-like structures were examined by H&E staining. Statistical analysis was conducted in GraphPad Prism (v5.1) using unpaired Student's t-tests and one-way ANOVA.ResultsHypoxic culture conditions better maintained naïve RPC characteristics than normoxic conditions, with significantly higher expression of RAX, LHX2, and PAX6. The addition of Matrigel to simulate the subretinal space supported RPE survival. In this 3D coculture model, RPCs differentiated into neural tube-like structures-potentially due to RPE-secreted inductive factors-and exhibited higher expression of photoreceptor markers than RPCs cultured without RPE.ConclusionOur results demonstrate that a 3D coculture system can be used to investigate the cellular and molecular mechanisms underlying retinal development and to support the formation of 3D retinal structures for future preclinical and clinical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"273-284"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin/β-cyclodextrin complex-loaded porous microneedles with enhanced mechanical properties for infected wound healing.","authors":"Bing Wei, Haojie Qiu, Guoqing Yan, Xuefeng Yang","doi":"10.1177/08853282261415599","DOIUrl":"10.1177/08853282261415599","url":null,"abstract":"<p><p>Porous microneedles (PMNs) can efficiently load drugs via capillary action within their porous structure and enable controlled drug release in deep wound layers, thereby significantly promoting wound healing. However, increasing the porosity of PMNs typically compromises mechanical strength, leading to needle tip deformation during skin penetration. To overcome this limitation, a mechanically robust porous microneedle (PMN) was fabricated by lyophilizing a chemically and physically dual-crosslinked hyaluronic acid/polyvinyl alcohol hydrogel formed <i>in situ</i> within a microneedle mold. The resulting PMN exhibited a single-needle bearing force exceeding the minimum threshold for skin penetration (>0.1 N) while maintaining a relatively high porosity (28.7%). Furthermore, a curcumin-loaded PMN (PMN@Cur) was easily prepared by adsorbing a curcumin/β-cyclodextrin complex solution into the abundant pores of PMN. The PMN@Cur showed sustained drug release, along with superior antioxidant and antibacterial activities. In infected wounds models, the PMN@Cur attained a wound contraction rate of up to 87.2% by day 14, demonstrating its high therapeutic efficacy in wound healing. These findings suggest that PMN@Cur, which combines high porosity with favorable mechanical properties, holds significant promise as a drug delivery system for advanced wound care.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"178-189"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zoledronic acid/cisplatin-loaded cucurbit[7]uril-modified upconversion nanoformulations drive tumor-associated macrophage polarization and potentiate the efficacy of cancer immunotherapy.","authors":"Yakun Ge, Pengcheng Li, Jiatong Zhang, Ruolan Wang, Renwen Zhang, Hongyu Xu, Zhenming Hao, Yuanxin Zhang","doi":"10.1177/08853282261422838","DOIUrl":"10.1177/08853282261422838","url":null,"abstract":"<p><p>Tumor-associated macrophages (TAMs) play a pivotal role in establishing a tumor immunosuppressive microenvironment (TIME) by inducing a phenotypic shift in macrophage from the pro-inflammatory M1 to the anti-inflammatory M2 phenotype. This polarization facilitates tumor growth, progression, metastasis, immune evasion, and chemoresistance. Consequently, reprogramming the TIME by repolarizing TAMs has emerged as a promising approach in cancer therapy. In this study, we synthesized core-shell structured nanoparticles (UCNPs@CB-Zol-Pt) utilizing host-guest interactions between NaYF₄:Yb/Er (UCNPs) and cucurbit[7]uril (CB). These nanoparticles were designed to polarize M2-like macrophages into M1-like macrophages and release tumor-associated antigens (TAAs), thereby potentially inducing the release of immunogenic cell death (ICD). Furthermore, the M1-type macrophages could ingest, process, and present TAAs generated from platinum-based chemotherapy through MHC class II molecules. This process simultaneously enhances the infiltration of helper and effector T cells into the TIME, thereby potentiating the efficacy of checkpoint blockade immunotherapy.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"259-272"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-synthesized silver-alginate-ciprofloxacin hydrogel: A multifaceted therapeutic for enhanced diabetic and burn Wound healing.","authors":"Kirti Singh, Virendra Bahadur Yadav, Mansi Rai, Gopal Nath, Anchal Srivastava, Preeti Suman Saxena, Ajay Vikram Singh","doi":"10.1177/08853282261418176","DOIUrl":"10.1177/08853282261418176","url":null,"abstract":"<p><p>Wound healing is a complex physiological process involving coordinated phases of inflammation, tissue repair, and scar formation to restore tissue integrity. Recently, silver nanoparticles (AgNPs) have been investigated for their potential to promote wound healing; however, their application is limited by concerns over cytotoxicity and the development of bacterial resistance. To address these limitations, this study presents the development of a silver-alginate hydrogel (AgSACip) incorporating ciprofloxacin, synthesized using green tea extract act as a biogenic reducing and stabilizing agent. Sodium alginate, a naturally derived macromolecule, serves as a biocompatible matrix to stabilize AgNPs, reduce cytotoxicity, and enhance therapeutic efficacy. The wound healing potential of AgSACip was evaluated in diabetic and burn wound models, and its antibacterial activity, cytotoxic effects, and pharmacokinetics were analyzed using in silico methods. The results demonstrate that AgSACip significantly accelerates wound healing compared to AgNPs and ciprofloxacin alone, particularly in diabetic and burn wounds. Furthermore, AgSACip exhibited enhanced antibacterial activity against bacterial strains isolated from wound pus samples and showed no cytotoxicity toward NIH-3T3 fibroblast cells. These findings highlight the potential of AgSACip, a macromolecule-based hydrogel, as a safe and effective alternative to conventional wound dressings, offering improved wound healing outcomes.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"285-303"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146003523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lipoic acid and melatonin co-loaded chitosan nanoparticles in alginate hydrogel: A multifunctional dressing for diabetic wound management.","authors":"Yankui Liu, Guang Wang, Xinhui Hu, Zhaoxiang Wu","doi":"10.1177/08853282251413117","DOIUrl":"10.1177/08853282251413117","url":null,"abstract":"<p><p>BackgroundImpaired wound healing in diabetic patients remains a major clinical challenge due to oxidative stress, chronic inflammation, and compromised tissue regeneration.ObjectiveThis study aimed to develop and evaluate a novel nanocomposite hydrogel system incorporating lipoic acid and melatonin to enhance diabetic wound healing.MethodsChitosan nanoparticles co-loaded with lipoic acid and melatonin (LAMELCNPs) were embedded within a calcium alginate hydrogel to form a bioactive wound dressing (LAMELCNPHYD). The system was characterized in terms of its microstructure, swelling behavior, drug release profile, cytocompatibility, antioxidant capacity, anti-inflammatory activity, and hemocompatibility. <i>In vivo</i> wound healing efficacy was assessed using a streptozotocin-induced diabetic rat model.ResultsScanning electron microscopy (SEM) confirmed the porous hydrogel structure. LAMELCNPHYD showed sustained drug release, excellent cytocompatibility, and enhanced antioxidant and anti-inflammatory activity. <i>In vivo</i>, LAMELCNPHYD significantly improved wound closure, collagen deposition, epithelial regeneration, and modulation of inflammatory markers (reduced interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and matrix metalloproteinase-9 (MMP-9); increased transforming growth factor-beta 1 (TGF-β1) and vascular endothelial growth factor (VEGF)).ConclusionThe LAMELCNPHYD hydrogel demonstrated potent wound healing efficacy through combined antioxidative, anti-inflammatory, and regenerative mechanisms, offering a promising therapeutic strategy for diabetic wound management.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"304-321"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145855994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}