Journal of Biomaterials Applications最新文献_第6页

Coacervation-responsive cubosome containing hyaluronic acid and albumin complex. 含有透明质酸和白蛋白复合物的凝聚反应性立方体。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-15 DOI: 10.1177/08853282251334466

Hyeon Ki Son, Panalee Pomseethong, Jin-Chul Kim

{"title":"Coacervation-responsive cubosome containing hyaluronic acid and albumin complex.","authors":"Hyeon Ki Son, Panalee Pomseethong, Jin-Chul Kim","doi":"10.1177/08853282251334466","DOIUrl":"10.1177/08853282251334466","url":null,"abstract":"Coacervation-responsive cubosomes were prepared by loading a complex of hydrophobically modified hyaluronic acid (HmHA) and hydrophobically modified albumin (HmAlb) and steviol glycoside (SG) into the water channels. Hyaluronic acid and albumin were modified with a lipid chain, and the HmHA and HmAlb were characterized by 1H NMR and FT-IR spectroscopy, respectively. The formation of the HmHA/HmAlb coacervate complex was optimized when the mass ratio was 1:9 under pH 4.0 conditions. The phase transition temperature of the cubic phase complex was observed to increase slightly from 60.9°C to 61.6°C as a result of the inclusion of the coacervate complex, as evidenced by differential scanning calorimetry. The maximum release degree of SG at 22°C was suppressed to 30.9% due to the coacervate at pH 3, and it was promoted to 75.9% at pH 5.5 due to the dissolution of the electrostatic complex as the pH value increased. The monoolein of the cubosDome enhanced the in vitro skin permeation of the cubosomal SG, as it could play a role as a skin permeation enhancer. The coacervation-responsive cubosome could be potentially used as a drug carrier that can release its content in a pH-controlled manner.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"252-267"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a ROS-responsive, glutathione-functionalized injectable hydrogel system for controlled drug release. 开发一种ros反应，谷胱甘肽功能化的可注射水凝胶系统，用于控制药物释放。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-10 DOI: 10.1177/08853282251334208

Kai Hu, Linlin Liang, Jian Song

{"title":"Development of a ROS-responsive, glutathione-functionalized injectable hydrogel system for controlled drug release.","authors":"Kai Hu, Linlin Liang, Jian Song","doi":"10.1177/08853282251334208","DOIUrl":"10.1177/08853282251334208","url":null,"abstract":"Oxidative stress arises from an imbalance between excessive production of reactive oxygen species (ROS) and the body's antioxidant defenses. In neurodegenerative diseases, this imbalance leads to ROS accumulation, causing neuronal dysfunction and cell death. Traditional drug therapies often fail to address the dynamic nature of neuroinflammation, limiting their therapeutic efficacy. To overcome this challenge, we have developed an innovative ROS-responsive injectable hydrogel. This hydrogel is designed to detect oxidative stress sensitively and release glutathione in a controlled manner, thereby modulating inflammation and restoring the damaged immune microenvironment to facilitate tissue repair. The hydrogel was synthesized by crosslinking polyvinyl alcohol (PVA) with sodium alginate modified with 3-aminophenylboronic acid (Alg-PBA). We investigated the hydrogel's formation mechanism and analyzed how component variations affect its morphological and rheological properties. Our findings demonstrate that an optimal Alg-PBA to PVA weight ratio of 2:1 yields a hydrogel with superior mechanical strength. Glutathione (GSH) release studies confirmed the hydrogel's pronounced ROS-responsive drug release behavior. Furthermore, biocompatibility assessments revealed that the hydrogel loaded with 100 μg/mL GSH exhibited excellent compatibility and significantly inhibited neuronal apoptosis under oxygen-glucose deprivation (OGD) conditions. This work presents a promising strategy for treating inflammation-related diseases and provides valuable insights for designing next-generation hydrogels that adapt to injury-responsive microenvironments.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"181-191"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144005312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A numerical study on mechanical and permeability properties of novel design additive manufactured Titanium based metal matrix composite bone scaffold for bone tissue engineering. 新设计的增材制造钛基金属基复合骨支架的力学性能和渗透性能的数值研究。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-15 DOI: 10.1177/08853282251333237

Umanath Puthillam, Renold Elsen Selvam

{"title":"A numerical study on mechanical and permeability properties of novel design additive manufactured Titanium based metal matrix composite bone scaffold for bone tissue engineering.","authors":"Umanath Puthillam, Renold Elsen Selvam","doi":"10.1177/08853282251333237","DOIUrl":"10.1177/08853282251333237","url":null,"abstract":"A novel design was developed for extrusion based additive manufacturing (robocasting) of bone scaffolds and a numerical study was carried out to find the optimal design to develop a bone scaffold for critical bone defect treatments. Initially, Representative Volume Analysis (RVE) analysis was carried out to predict the Young's modulus (E) of Titanium + Calcium Silicate and Titanium + Hydroxyapatite composites. The RVE analysis outputs were used to find out the E value of various bone scaffold designs and material compositions. The novel stepped design could be used to tailor the mechanical and biological properties of the scaffold by altering the contact support area between strands and changing the pore size, shape and orientation to control the permeability and nutrient transportation. The test revealed that some of the designed scaffolds are suitable for developing scaffolds for cortical bone defects as the E value lies between 10 and 30 GPa. The CFD analysis indicated that some designs do not possess the permeability required for a scaffold to aid nutrient transportation which is ideally between 1.5 × 10-9 and 5 × 10-8 m2. A sample model was printed and sintered in an argon atmosphere using a microwave furnace to check the feasibility of the process.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"268-283"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel two-step conversion from DCPD-coated β-TCP to low crystallinity β-TCP porous scaffolds via combination between dry heating and hydrothermal methods: Effects on pre-osteoblast cell responses. 干热与水热相结合，从dcpd包被β-TCP到低结晶度β-TCP多孔支架的新型两步转化：对成骨前细胞反应的影响。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-08 DOI: 10.1177/08853282251333231

Ahmed Hafedh Mohammed Mohammed, Khairul Anuar Shariff, Mohamad Hafizi Abu Bakar, Ali A Salman, Aira Matsugaki, Takayoshi Nakano, Intan Nirwana, Alexander Patera Nugraha

{"title":"A novel two-step conversion from DCPD-coated β-TCP to low crystallinity β-TCP porous scaffolds via combination between dry heating and hydrothermal methods: Effects on pre-osteoblast cell responses.","authors":"Ahmed Hafedh Mohammed Mohammed, Khairul Anuar Shariff, Mohamad Hafizi Abu Bakar, Ali A Salman, Aira Matsugaki, Takayoshi Nakano, Intan Nirwana, Alexander Patera Nugraha","doi":"10.1177/08853282251333231","DOIUrl":"10.1177/08853282251333231","url":null,"abstract":"This study presents a novel two-step process to fabricate low crystallinity (LC) β-tricalcium phosphate (β-TCP) porous scaffolds and evaluates their implications for pre-osteoblast cell responses. The novelty of this study lies in the two-step conversion of dicalcium phosphate dihydrate (DCPD) -coated β-TCP porous scaffold into LC β-TCP porous scaffolds through a combination of dry heating and hydrothermal conditions at 200°C. The obtained LC β-TCP porous scaffolds were characterised using a Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD), Fourier-Transform Infrared (FTIR), porosity, and compressive strength analysis confirmed the successful fabrication of LC β-TCP scaffolds. Besides, in vitro tests using pre-osteoblast MC3T3-E1 cells were conducted to investigate the cell responses toward LC β-TCP porous scaffolds. The results revealed that the LC β-TCP porous scaffolds were successfully fabricated by converting the DCPD-coated β-TCP into the dicalcium phosphate anhydrous (DCPA) coated β-TCP, followed by a hydrothermal process in a 0.1 mol/L calcium chloride (CaCl2) aqueous solution at 200°C for 24 hours to obtain LC of pure β-TCP scaffold. Moreover, in vitro cell study indicated that the cell density and proliferation surrounding the surface of the LC β-TCP porous scaffold were greater than DCPD-coated β-TCP porous scaffolds. The findings from this study are expected to significantly impact bioceramic technology by enhancing cell responses.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"218-235"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143811518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-inflammatory and antioxidant effects of haematococcus carbon dots in ulcerative colitis: A nanoparticle-based approach. 碳红球菌点在溃疡性结肠炎中的抗炎和抗氧化作用：一种基于纳米粒子的方法。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-13 DOI: 10.1177/08853282251333240

Yuting Liu, Mengqing Wang, Chaoyan Zhang

引用次数: 0

3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects. 3d打印纳米羟基磷灰石/聚（乳酸-羟基乙酸）脂肪源间充质干细胞支架增强骨缺损大鼠骨再生

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-04-03 DOI: 10.1177/08853282251332050

Hai Yan, Fei Xia

{"title":"3D-printed nano-hydroxyapatite/poly(lactic-co-glycolic acid) scaffolds with adipose-derived mesenchymal stem cells enhance bone regeneration in rat model of bone defects.","authors":"Hai Yan, Fei Xia","doi":"10.1177/08853282251332050","DOIUrl":"10.1177/08853282251332050","url":null,"abstract":"Three-dimensional (3D) printing has huge potential in the medical field, including bone tissue engineering scaffolds. In our study, the 3D-printed poly (lactic-co-glycolic acid) (PLGA)/nano-hydroxyapatite (HA) scaffolds carrying adipose-derived mesenchymal stem cells (ADMSCs) were constructed, and whether such scaffolds have therapeutic potential in bone defects was investigated. For in vitro assays, rat ADMSCs were implanted into blank cell wells (Blank) and PLGA/nHA and ADMSCs/PLGA/nHA scaffolds. The vitality and proliferation of ADMSCs were detected through calcein-AM/PI staining and CCK-8 assay to assess the biocompatibility of the scaffolds. ADMSCs in three groups were incubated in osteogenic induction medium, and ALP and ARS staining were performed after 7 days and 21 days, respectively. Runx2, Osterix, OCN, and OPN mRNA expression in ADMSCs was detected through RT-qPCR. For in vivo assays, rat models of radius defects were implanted by PLGA/nHA scaffolds or ADMSCs/PLGA/nHA scaffolds, and micro-CT scan analysis was conducted at week 12 after implantation. Bone marrow cell formation and Runx2 expression in rat radius tissues were evaluated through H&E and immunohistochemical staining, respectively. The results showed that ADMSCs/PLGA/nHA scaffolds provided transplanted cells with a stable carrier as well as maintained their activity and facilitated their proliferation. ADMSCs/PLGA/nHA promoted ADMSC osteogenic differentiation in vitro. Besides, the implantation of ADMSCs/PLGA/nHA scaffolds improved bone regeneration, enhanced bone marrow cell formation, and increased Runx2 expression in rat models of radius defects. Collectively, the 3D-printed ADMSCs/PLGA/nHA scaffolds effectively promote ADMSC osteogenic differentiation and exhibit significant bone repair effects, suggesting its therapeutical potential for bone defects.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"284-296"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143780094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silk fibroin-hyaluronic acid nanofibers for peripheral nerve regeneration. 丝素-透明质酸纳米纤维用于周围神经再生。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-08-01 Epub Date: 2025-03-25 DOI: 10.1177/08853282251329315

Madeline R Badrak, Judy Senanayake, Ahmad Zunnu Rain, Harini G Sundararaghavan

{"title":"Silk fibroin-hyaluronic acid nanofibers for peripheral nerve regeneration.","authors":"Madeline R Badrak, Judy Senanayake, Ahmad Zunnu Rain, Harini G Sundararaghavan","doi":"10.1177/08853282251329315","DOIUrl":"10.1177/08853282251329315","url":null,"abstract":"Peripheral nerve injuries are common and a major source of pain that can lead to debilitating loss of function. Current treatments are limited, with autologous nerve grafts being the gold standard treatment for nerve injuries. However, autografting is not always successful and can lead to increased debilitation through donor site morbidity. Tissue engineering seeks to improve nerve injury treatment though the use of nerve conduits. Conduits made from a functional biomaterial can be implanted into a nerve injury site encouraging and controlling axonal regrowth without causing additional harm to the patient. Both silk fibroin (SF) and hyaluronic acid (HA) have been proven successful in the field of neural tissue engineering. SF has excellent mechanical properties and is biocompatible. HA is part of the extracellular matrix and had been used in nerve regeneration applications. This study developed aligned combination SF-HA nanofibers through electrospinning that could be used within a nerve conduit. Both materials were methacrylated to allow for photocrosslinking and additional control over material properties. Methcrylated SF-HA was tested alongside a material containing only methacrylated HA that has already proven to be effective in literature. When characterizing the materials, it was found that through chemical methacrylation HA was substituted at 60% while SF reported a 30% substitution. Electrospun SF-HA nanofibers were found to have a greater diameter than HA fibers; however, SF-HA was found to be more aligned with greater surface hydrophobicity. Mechanically, it was found that both materials exceeded the elastic modulus of native tissue, but SF-HA far exceeded HA in elasticity and overall fiber extension. Furthermore, human Schwann cells attached, proliferated, and released more pro-regenerative growth factors on SF-HA than HA. Dorsal root ganglia neurons also displayed longer neurite extensions on SF-HA fibers. We concluded that SF-HA nanofibers have potential as a nerve conduit material.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"307-323"},"PeriodicalIF":2.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced thermal damage with electrical conductive hydrogel in radiofrequency (RF)-induced intestinal fusion. 导电水凝胶在射频（RF）诱导的肠融合中减少热损伤。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-07-14 DOI: 10.1177/08853282251360948

Zhongxin Hu, Lin Mao, Xuyan Liu, Wenwen Zhang, Chengli Song

{"title":"Reduced thermal damage with electrical conductive hydrogel in radiofrequency (RF)-induced intestinal fusion.","authors":"Zhongxin Hu, Lin Mao, Xuyan Liu, Wenwen Zhang, Chengli Song","doi":"10.1177/08853282251360948","DOIUrl":"https://doi.org/10.1177/08853282251360948","url":null,"abstract":"Radiofrequency (RF)-induced tissue thermal fusion is an innovative method of tissue anastomosis without the need for sutures or staples. However, this technology is generally challenged by significant tissue thermal damage, which may cause tissue necrosis, severely hindering its clinical application. Here, we reported a feasible approach of using a conductive polyacrylamide-alginate-0.5 M calcium ion (denoted as PAAm-Alg-0.5Ca2+) hydrogel during tissue fusion process, aiming to reduce thermal damage to the tissues. Electrothermal simulation results indicated that the PAAm-Alg-0.5Ca2+ hydrogel effectively reduced the fusion temperature of about 40°C, and produced less thermal damage to adjacent tissues (14.7%). In addition, the result of ex-vivo tissue fusion experiment demonstrated that the welded tissue reached an optimum temperature of about 100°C, and the fusion site exhibited a favorable morphology without tissue carbonization and structure breakdown. Furthermore, the fused tissue with the application of hydrogel could endure a burst pressure of 14.51 ± 1.27 kPa, which was much higher than the normal human intestinal pressure (0.67-6.67 kPa). The histomorphological examination also indicated a comparatively integrated structure and a high collagen volume fraction of fused tissues. Therefore, the application of PAAm-Alg-0.5Ca2+ hydrogel in RF-induced tissue fusion is considered an efficient and secure method of reducing excess thermal damage while maintaining anastomotic strength of tissues.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251360948"},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Making vancomycin a potent broad-spectrum antimicrobial agent using polyaziridine-stabilized gold nanoparticles as a delivery vehicle. 利用聚氮吡啶稳定的金纳米颗粒作为递送载体，使万古霉素成为一种有效的广谱抗菌剂。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-07-01 Epub Date: 2025-03-15 DOI: 10.1177/08853282251327486

Atul Kumar Tiwari, Aishwarya Nikhil, Avinash Chaurasia, Prem C Pandey, Roger J Narayan, Munesh Kumar Gupta

{"title":"Making vancomycin a potent broad-spectrum antimicrobial agent using polyaziridine-stabilized gold nanoparticles as a delivery vehicle.","authors":"Atul Kumar Tiwari, Aishwarya Nikhil, Avinash Chaurasia, Prem C Pandey, Roger J Narayan, Munesh Kumar Gupta","doi":"10.1177/08853282251327486","DOIUrl":"10.1177/08853282251327486","url":null,"abstract":"The rise of antimicrobial drug resistance among microorganisms presents a global challenge to clinicians. Therefore, it is essential to investigate drug delivery systems to combat resistant bacteria and fungi. This study examined the potential and mode of action of vancomycin-conjugated gold nanoparticles (PEI-AuNP@Van) to enhance vancomycin's biocidal activity against C. tropicalis, C. albicans, E. coli, and P. aeruginosa. Drug conjugation and nanoparticle characterization were assessed using UV-Vis spectroscopy, X-ray diffraction, TEM, ATR-FTIR, and fluorescence spectroscopy. Effective vancomycin conjugation on polyethyleneimine-stabilized gold nanoparticles was achieved via electrostatic interactions or hydrogen bonding between the COO-/OH groups of vancomycin and the NH- groups of polyethyleneimine, yielding nanoparticles with a narrow size distribution and high zeta potential. The high luminescence of the nanoparticles facilitated their detection in microbial cells. PEI-AuNP@Van was internalized in C. albicans and C. tropicalis but showed surface adsorption in E. coli and P. aeruginosa. The in vitro results indicated that the nanodelivery system exhibited superior biocidal activity against the tested strains compared to free vancomycin and unconjugated AuNPs. The mode of action of PEI-AuNP@Van was cell-type-dependent, involving intracellular reactive oxygen species accumulation, cell membrane integrity loss, and apoptosis. The development of antimicrobial nanoformulations using AuNPs and efficient conjugation systems offers a promising approach to address antimicrobial drug resistance.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"145-162"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095888/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caprine dermal scaffolds for repair of full-thickness skin wounds in rabbits. 山羊真皮支架修复兔全层皮肤创面。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-07-01 Epub Date: 2025-03-20 DOI: 10.1177/08853282251329559

Parvez Ahmad, Sangeeta Devi Khangembam, Anil Kumar Gangwar, Vipin Kumar Yadav, Prafull Kumar Singh, Yogendra Singh, Ravi Prakash Goyal, Surendra Pratap Chakraverty, Rajesh Kumar Verma

{"title":"Caprine dermal scaffolds for repair of full-thickness skin wounds in rabbits.","authors":"Parvez Ahmad, Sangeeta Devi Khangembam, Anil Kumar Gangwar, Vipin Kumar Yadav, Prafull Kumar Singh, Yogendra Singh, Ravi Prakash Goyal, Surendra Pratap Chakraverty, Rajesh Kumar Verma","doi":"10.1177/08853282251329559","DOIUrl":"10.1177/08853282251329559","url":null,"abstract":"Biological scaffolds prepared after decellularization are used for the restoration of damaged tissues. A number of chemicals are used for bioscaffold preparation, and some of them damage their composition and architecture. Herein, we investigated the Sapindus mukorossi fruit pericarp extract (SPE) (5%) for decellularization of the caprine dermis. The dermal samples were processed in 5% SPE over magnetic stirrer for 96h at room temperature. The decellularization efficiency of SPE was analyzed by histological examination, DAPI staining, scanning electron microscopy (SEM), quantification of DNA hydroxyproline and hemocompatibility determination. Further, these acellular caprine dermal scaffolds were transplanted on full thickness skin wounds of group III New Zealand white rabbits. The wounds were left open in group I (Sham) and reconstructed by autograft in group II (n = 6 in each group). Continuous agitation of native caprine dermal tissues in 5% SPE for 96 hours leads to complete decellularization without affecting the extracellular matrix architecture. Microscopic observation of decellularized samples did not show any nuclei. DNA quantity was reduced (p < .05) in decellularized samples and scaffolds were found to be hemocompatible. Complete healing was observed on day 28 in groups II and III. No significant difference was noted in IgG in all the groups. Quantitative assessment of MDA showed a significant increase in groups I and II. Our results suggested that the 5% SPE solution effectively decellularized the native caprine dermis and the scaffolds were well tolerated by the animals.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"36-49"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0