Journal of Biomaterials Applications最新文献

{"title":"Six-axis robotic extrusion of hybrid hydrogels for biomimetic airway model fabrication.","authors":"Jennifer O'Neil, Larry A Villasmil-Urdaneta, Perrin Woods, Riley Rohauer, Ahasan Habib","doi":"10.1177/08853282261421467","DOIUrl":"10.1177/08853282261421467","url":null,"abstract":"<p><p>Respiratory diseases remain a major global health burden, motivating the need for improved experimental lung models that capture both anatomical geometry and mechanical compliance. Traditional three-axis 3D printers face limitations in replicating the lung's curving, branching structures, often resulting in pore collapse or loss of fidelity. In this study, we demonstrate the use of a six-axis robotic extrusion bioprinter to fabricate anatomically inspired airway structures using hybrid hydrogels composed of Alginate (A) and CarboxyMethyl Cellulose (CMC). By systematically tuning hydrogel formulations, we identified a blend (5% Alginate-7% CMC, <i>i.e.</i>, A₅C₇) that provides a balance of viscosity, shear-thinning, and diffusion resistance, resulting in enhanced print fidelity and structural stability compared to single-polymer inks. Using this formulation, the robotic platform successfully printed tubular and bifurcating airway constructs that retained lumen geometry, withstanding axial and diametral compression within ranges relevant to lung tissue mechanics. Printability (Pr ≈ 0.92-1.08) analysis confirmed consistent pore fidelity, while mechanical testing demonstrated elastic recovery under loading. A preliminary aerosol deposition test highlighted the feasibility of coupling these constructs with drug delivery studies, though more sensitive measurement methods will be required. Collectively, this work establishes a proof-of-concept fabrication platform for anatomically accurate and mechanically compliant airway models, which can be adapted in future studies to represent both healthy and pathological respiratory states through targeted modifications in geometry, material composition, and cellular integration.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"201-220"},"PeriodicalIF":2.4,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146093237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoting healing of <i>Acinetobacter baumannii</i> infected cutaneous wounds under hyperglycaemic condition via antibacterial and haemolytic Ag-Collagen/Al₂O₃/Cellulose nanodressings.","authors":"Swati Sucharita Singh, Subhra Sourav Dash, Sankha Chakrabortty, Susanta Kumar Behera, Ramesh Kumar, Byong-Hun Jeon, Suraj K Tripathy, Cecilia Stålsby Lundborg, Amrita Mishra, Moonis Ali Khan","doi":"10.1177/08853282261475097","DOIUrl":"https://doi.org/10.1177/08853282261475097","url":null,"abstract":"<p><p>Chronic diabetic wounds pose a persistent clinical problem due to ongoing infections and impaired lifestyle. Traditional silver-based therapies are often constrained by cytotoxicity and variable healing outcomes, underscoring the need for safer, more efficacious wound care approaches. This study emphasizes the design and assessment of a multifunctional Ag-Collagen/Al₂O₃/Cellulose nanodressing, particularly for diabetic wound therapy. The Ag-Collagen nanocomposites were incorporated into an Al₂O₃-reinforced Cellulose scaffold to serve as an adsorbent, a biocompatible substrate, a mechanical stabilizer, and a prolonged antibacterial efficacy, achieving >90% biofilm inhibition against <i>Acinetobacter baumannii</i>. Transcriptomic analysis (log₂ fold change ≥ 1, <i>p</i> ≤ 0.05) revealed significant dysregulation of genes involved in metabolic pathways, membrane integrity, stress response, and virulence, suggesting that nanodressing induces oxidative and metal stress while concurrently disrupting vital cellular functions. These molecular modifications indicate diminished bacterial adaptability and reduced resistance. The nanodressing exhibited significant cytocompatibility with RAW 264.7 and 3T3 cells, as well as superior hemocompatibility, indicated by red blood cell lysis remaining below 5%. In vivo wound-healing investigations in normal and diabetic murine models demonstrated excellent infection control and expedited wound closure, achieving 90%-95% closure within 21 days. The Ag-Collagen/Al₂O₃/Cellulose nanodressing is a biocompatible antibacterial platform for multidrug-resistant diabetic wounds.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261475097"},"PeriodicalIF":2.4,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148630677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of polydopamine coated silk fibroin methacryloyl hydrogel loaded with cell free fat extract in diabetic wound healing.","authors":"Qinhao Gu, Zexin Fu, Lu Wang, Chengrui Guo, Yi Sun, Sufan Wu, Ji Wang, Bowen Zheng, Wei Zhang","doi":"10.1177/08853282261474628","DOIUrl":"https://doi.org/10.1177/08853282261474628","url":null,"abstract":"<p><p>Diabetic foot ulcers (DFUs) are common and serious problems in diabetes patients. They also represent one of the leading causes of non-traumatic lower limb amputation worldwide. Despite improvements in clinical care, numerous standard therapies fail to completely close chronic diabetic wounds. Cell-free fat extract (Ceffe) aids tissue repair and angiogenesis but its clinical worth is greatly reduced by rapid diffusion and enzymatic degradation in the wound. Silk fibroin methacryloyl (SilMA) can be formulated into a photocrosslinked hydrogel capable of sustained delivery of bioactive factors, whereas polydopamine (pDA) provides durable wet-tissue adhesion. In this work, we developed a multifunctional composite hydrogel pSilMA@Ceffe (polydopamine-coated SilMA hydrogel loaded with Ceffe) that integrates the prolonged release properties of SilMA with the tissue-binding strength of pDA to enhance healing of refractory diabetic wounds. We prepared and characterized pSilMA@Ceffe with respect to physicochemical properties, printability, and release kinetics. Cellular assays and diabetic mouse models employed used to assess biocompatibility. Cell proliferation, migration, and tube formation were evaluated using in vitro assays, while full-thickness skin wounds in db/db mice were used to determine in vivo efficacy. Wound closure was monitored at multiple time points, and tissue regeneration was assessed via H&E, Masson's trichrome, and immunofluorescent staining for CD31, keratin 1 (K1), Ki-67, and type I collagen (Col-1). The final hydrogel had satisfactory 3D printability, stable tissue adhesion, and constant long-term release of Ceffe. It supported cell survival and significantly enhanced proliferation, migration, and angiogenic activity in culture. In diabetic mice, pSilMA@Ceffe accelerated wound healing, neovascularization, and cell proliferation, and promoted more organized collagen deposition in the regenerating dermis. Combining photocrosslinkable handling with robust pro-regenerative activity, pSilMA@Ceffe represents an effective and translatable candidate for the clinical treatment of chronic diabetic wounds.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261474628"},"PeriodicalIF":2.4,"publicationDate":"2026-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148630609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> and <i>in vivo</i> evaluation of a bioactive polysaccharide based self-healing hydrogel film for enhanced wound healing.","authors":"Ayan Ranjan Hati, Chalamala Balagangadhar Reddy, Purnendu Ghosh, Kaushik Mukherjee, Amalesh Samanta, Samit Kumar Nandi, Tapan Kumar Giri","doi":"10.1177/08853282261474149","DOIUrl":"https://doi.org/10.1177/08853282261474149","url":null,"abstract":"<p><p>The objective of this research was to develop a carboxymethyl chitosan (CMCS) loaded borax cross-linked guar gum (GG) self-healing hydrogel film dressing for enhanced wound healing. Cross-links between borax and GG were confirmed through FTIR, SEM, and zeta potential measurement. The optimized formulation demonstrated favourable characteristics, including a thickness of 0.25 ± 0.008 mm, a water vapour transmission rate of 592.9 ± 55.1 g m^-2 day^-1, tensile strength of 37 ± 1 MPa in the wet state and 61.33 ± 4.16 MPa in the dry state, a swelling ratio of 557.84%, and 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of 53.132 ± 1.02%. Furthermore, the optimized formulation exhibited a healing efficiency of 43.949 ± 0.56%, as determined by tensile strength measurements of the self-healed hydrogel film dressings after 6 h. Antimicrobial testing revealed broad-spectrum activity against wound-associated pathogens, including <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. The therapeutic efficacy was evaluated through <i>in vivo</i> experiments using a rat full-thickness wound model, which demonstrated that the optimized formulation achieved greater wound closure than commercial Tegaderm and ofloxacin loaded dressings. The CMCS-loaded hydrogel film dressing showed significantly higher hydroxyproline levels than the commercial Tegaderm and ofloxacin dressings. Hydroxyproline levels increased from day 7 and day 14, indicating progressive collagen synthesis. Histopathology analysis revealed that the CMCS-loaded hydrogel film dressing promoted faster re-epithelialization, reduced inflammatory cell infiltration, and enhanced granulation tissue formation compared to Tegaderm and ofloxacin dressings.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261474149"},"PeriodicalIF":2.4,"publicationDate":"2026-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148619896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study on the homeostasis mechanism of in vivo degradation of PCL microsphere fillers.","authors":"Shuang Wu, Qi Guo, Rongling Liu, Zhisheng Yan, Ranran Tang, Shuo Pang, Wenjie Chen, Tun Yuan","doi":"10.1177/08853282261469759","DOIUrl":"https://doi.org/10.1177/08853282261469759","url":null,"abstract":"<p><p>Polycaprolactone (PCL) has been widely applied in the fields of medical aesthetics and clinical filling due to its excellent biocompatibility, controllable degradability, and favorable mechanical properties. However, the high crystallinity and hydrophobicity result in a longer degradation period, making it more suitable for long-term and sustained filling treatments. Yet it is difficult to observe the degradation endpoint in a short period of time, which poses a challenge for the safety and efficacy evaluation of the product before its market launch. At present, the differences in in vivo behavior among various PCL microspheres and their clinical implications remain incompletely understood. In this study, a subcutaneous implantation model in rabbits was employed to investigate the in vivo degradation patterns of two types of PCL microsphere fillers and the material-tissue interactions contributing to the establishment of bodily homeostasis. The results demonstrated that PCL microsphere fillers achieve a homeostasis of safety at 12 months through a sequential process involving \"surface erosion-cellular involvement-collagen encapsulation.\" Furthermore, they maintain a homeostasis of efficacy for at least 30 months via a collagen remodeling strategy characterized by \"initial reinforcement, subsequent shaping, and eventual equilibration,\" thereby forming a predictable dynamic filler construct. These findings provide a mechanistic basis for individualized clinical application and quality evaluation.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261469759"},"PeriodicalIF":2.4,"publicationDate":"2026-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148411955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental study on the repair of rabbit femoral condyle critical-sized bone defect using polyphosphoester-amino acid copolymer-composite nanohydroxyapatite filling material.","authors":"Mingzhong Xiao, Hua Gao, Hui Dong, Yan Xiong, Zeping Yu, Wenli Zhang, Hong Duan","doi":"10.1177/08853282261468122","DOIUrl":"https://doi.org/10.1177/08853282261468122","url":null,"abstract":"<p><p>To engineer a porous nanohydroxyapatite/polyphosphoester-amino acid composite bone graft substitute (nHA/PPE-AA) in granular form and evaluate its reparative efficacy and in vivo safety in a rabbit femoral condyle critical-sized defect (CSD) model. Porous nHA/PPE-AA granules were fabricated using polyphosphoester-amino acid copolymer (PPE-AA) as the organic matrix and nanohydroxyapatite (nHA) as the inorganic phase. Material characteristics were examined by scanning electron microscopy, X-ray diffraction, and Fourier-transform infrared spectroscopy. A rabbit femoral condyle CSD model (10 × 10 × 10 mm³) was randomly assigned to nHA/PPE-AA, positive control (calcium sulfate), and blank groups. Bone repair was assessed by gross inspection, serum bone turnover markers, digital radiography (G/N ratio), micro-computed tomography, sequential fluorochrome labeling, histology, interfacial SEM, and BMP-2 mRNA expression. Safety was evaluated by ESR, WBC, CRP, and histology of major organs and peri-implant tissues. The nHA/PPE-AA composite exhibited an interconnected porous granular structure (35%-55% porosity, 3-7 MPa compressive strength). XRD and FTIR confirmed stable coexistence of nHA and PPE-AA. Gross and imaging analyses demonstrated progressive defect repair, with higher G/N ratios and more trabecular regeneration in the nHA/PPE-AA group. Fluorochrome labeling and histomorphometry indicated accelerated mineralization and shorter lag time. Histology and SEM confirmed mature neobone formation and superior bone-material integration. RT-qPCR showed sustained BMP-2 upregulation from 1 to 3 months, peaking at 3 months. Safety assessments revealed only transient changes in ESR, WBC, and CRP, with no significant organ pathology. Porous nHA/PPE-AA granules provide interconnected porous structure, moderate mechanical support, osteogenic activity, and good in vivo biocompatibility. In a rabbit femoral condyle CSD model, they promoted new bone formation, accelerated mineralization, and improved bone-material interface, highlighting their potential as a translationally relevant bone void filler.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282261468122"},"PeriodicalIF":2.4,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148404818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione-coated titanium implants promote bone defect repair through antioxidant stress.","authors":"Tianran Wang, Tao Jiang, Junyong Ge, Zhansong Tian, Shiwu Dong, Xiang Ao, Shouqi Sun, Yu Wang, Cheng Yu, Zhaxi Mima","doi":"10.1177/08853282251414273","DOIUrl":"10.1177/08853282251414273","url":null,"abstract":"<p><p>Elevated levels of reactive oxygen species (ROS) in the bone defect area impede bone regeneration and repair. Although titanium and its alloys are widely used for bone defect repair, they lack bioactivity and the ability to scavenge ROS at the defect site, leading to poor osseointegration. To address this, surface modification of Ti alloys with coatings is a common strategy. This study aimed to investigate the osteogenic and antioxidant effects of a novel glutathione (GSH)-coated Ti screw (Ti^GSH) on bone regeneration. Ti^GSH was fabricated using a PDA intermediary layer, followed by chelation with GSH. In vitro studies demonstrated sustained GSH release from Ti^GSH over 15 days. In vivo studies revealed that Ti^GSH did not alter the levels of GSH in the plasma of rats, but it significantly increased the GSH content at the bone defect site. Ti^GSH effectively scavenged DPPH• radicals and reduced intracellular ROS levels in bone marrow mesenchymal stem cells (BMSCs), promoting their proliferation, osteogenic differentiation, and mineralization. In vivo experiments in a rat bone defect model showed that Ti^GSH significantly reduced the level of oxidative stress at the bone defect site, thereby enhancing the bone regeneration and osseointegration capabilities of the titanium screw. Meanwhile, Ti^GSH did not cause any adverse effects in the rats. Overall, Ti^GSH demonstrates promising potential as a biocompatible and osteoinductive material for bone defect repair, with sustained local GSH release mitigating oxidative stress and promoting bone regeneration.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"106-122"},"PeriodicalIF":2.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145943971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicomponent scaffold with hierarchical porosity for enhanced mechanical and bioactivity.","authors":"Hong Chen, Ying Huang, Changzhi Huang, Yuanqing Cai, Yang Chen, Yonggang Yan, Xinyu Fang, Wenming Zhang","doi":"10.1177/08853282251411223","DOIUrl":"10.1177/08853282251411223","url":null,"abstract":"<p><p>The repair of critical-sized bone defects remains challenging due to insufficient blood vessel formation and nutrient delivery. To overcome this limitation, we developed a porous organic/inorganic composite scaffold, named Micro-MP, through a combined strategy of H₂O₂ gas foaming and freeze-drying. The scaffold incorporates an oxidized dextran/gelatin (OD/Gel) hydrogel with magnesium calcium phosphate cement (MCPC), forming a double network stabilized by multiple weak interactions. H₂O₂ plays a dual role by serving as both an oxidizing agent that strengthens the crosslinked network and a foaming agent that creates interconnected macropores. Subsequent freeze-drying introduces micropores within the macropore walls, resulting in a hierarchical pore architecture. Remarkably, the scaffold maintains comparable mechanical strength before and after foaming, as the oxidative function of H₂O₂ enhances network density. Furthermore, H₂O₂ treatment promotes apatite deposition on scaffold surfaces and improves protein adsorption capacity, thereby enhancing the attachment, proliferation, and osteogenic differentiation of rat bone marrow stromal cells (rBMSCs). This strategy effectively resolves the problem of maintaining mechanical strength during the foaming process while increasing pore size, offering a promising approach for bone regeneration.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"80-95"},"PeriodicalIF":2.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145834101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold-nanoparticle decorated allograft tendons confer mitigation of inflammatory and oxidative species in vitro.","authors":"Mark J Messler, Jonathan Austin, John Brockman, David A Grant, Sheila A Grant","doi":"10.1177/08853282251399594","DOIUrl":"10.1177/08853282251399594","url":null,"abstract":"<p><p>Millions of people suffer from traumatic ligament ruptures every year. Tears of the anterior cruciate ligament in the knee are the most common ligament tear requiring surgical intervention. Without surgical intervention, this type of injury can be debilitating, painful, and athletic career-ending. Furthermore, damage to the ACL can lead to troublesome, chronic complications such as accelerated progression of osteoarthritis, even with modern surgical intervention. Most commonly, patients have their torn or ruptured ACL reconstructed with the use of a tendon graft, either autograft or allografts. Both graft material can result in prolonged and painful healing with limited capacity for total remodeling of the graft. It is hypothesized that these grafts can improve healing through the use of gold nanoparticles conjugated to the grafts. The proposed mechanism of enhanced ligamentization is through reduced excessive levels of inflammation. The conjugation process and modified physical properties of the grafts were examined, as well the cellular response to these alterations. The results demonstrated that the AuNP conjugated tendon grafts had a significant effect on cellular oxidation and inflammation levels. Additionally, the cells were shown to be biocompatible with AuNP modified grafts, as evidenced by metabolic and proliferation assays, however there was a notable decrease in these measures especially at the higher AuNPs concentration. It appeared that a AuNP concentration of less than 50 g/g AuNP to tissue will elicit a positive biocompatibility response while still reducing inflammatory response.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"123-133"},"PeriodicalIF":2.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145648565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of biosynthesized gold nanoparticles with curcumin on motor, inflammatory, and oxidative parameters in a Parkinson's disease model.","authors":"Paulo Cesar Lock Silveira, Ligia Milanez Venturini, Gustavo de Bem Silveira, Igor Ramos Lima, Camila da Costa, Gabrielli Martins, Laura de Roch Casagrande, Ellen De Pieri, Paulo Emilio Feuser, Yaodong Gu, Ricardo Andrez Machado-de-Ávila, Anand Thirupathi","doi":"10.1177/08853282251409687","DOIUrl":"10.1177/08853282251409687","url":null,"abstract":"<p><p>Parkinson's disease is a progressive and irreversible neurodegenerative disease, whose main characteristic is the death of dopaminergic neurons. The animal model of Parkinsonism with reserpine has been used to mimic the consequences of Parkinson's disease. The use of gold nanoparticles in the treatment of several diseases arises due to their evidence of promising antioxidant and anti-inflammatory properties. Curcumin, in turn, presents neuroprotective activity in neurodegenerative diseases by reducing both inflammatory activity and oxidative stress in models of Parkinson's disease. This article seeks to understand the effects of the use of gold nanoparticles biosynthesized with curcumin in an experimental model of Parkinson's disease with reserpine. C57BL/6 mice were used, divided into five groups: I. Control; II. Parkinson's disease; III. Parkinson's disease + Gold nanoparticles; IV. Parkinson's disease + Curcumin; V. Parkinson's disease + Gold nanoparticles biosynthesized with curcumin. Mice were subjected to subcutaneous induction with reserpine, and treatments began 24 h after administration. Seven treatments were applied at 24-h intervals via the intranasal route. After the last treatment, behavioral tests were conducted, followed by euthanasia and removal of brain structures. The combined treatments demonstrated potential neuroprotective effects, reversing inflammatory and oxidative processes and partially reversing Parkinsonian behavior. Together, these therapies potentiated their effects, leading to near-complete reversal of reserpine-induced damage.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"134-148"},"PeriodicalIF":2.4,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145809928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}