Journal of Biomaterials Applications最新文献

{"title":"Novel fabrication of hydroxypropyl-β-cyclodextrin functionalized zein protein nanoparticles Co-encapsulated with bio-molecules to attenuate pregnancy-induced hypertension by inducing trophoblast cells proliferation with TLR4 signaling pathway.","authors":"Wang Yalin, Tong Xinyun, He Yin, Huang Ke, Luo Quanhui, Wang Jinxing, Wu Song","doi":"10.1177/08853282251322272","DOIUrl":"10.1177/08853282251322272","url":null,"abstract":"<p><p>Trophoblast dysfunction during pregnancy time is majorly involved to lead pathogenesis of preeclampsia. In the present investigation, the facile nanoformulation by Zein protein particles functionalized with hydroxypropyl-beta-cyclodextrin (β-CD) and co-encapsulated with curcumin and eugenol compounds (Cu/Eu@H-β-CD-ZNPs) is developed to achieve enhanced therapeutic potential in the treatment of preeclampsia. To investigate the positive trophoblast function, trophoblast cells were treated and observed for in vitro cell proliferation, invasion and migration ability under hypoxic condition. The Cu/Eu@H-β-CD-ZNPs have significantly induced the restoration ability of trophoblast cells. In vivo animal study was performed using pregnancy rat models by inducing LPS and observed the hypertension-related factors. The Cu/Eu@H-β-CD-ZNPs prominently down-regulated the expressions of serum and placental pro-inflammatory factors (IL-6, TNF-α, IL1β, and IFN-γ). Additionally, p65 and TLR4 protein expressions in LPS-induced model were effectively downregulated after administration of Cu/Eu@H-β-CD-ZNPs. Results of current investigation provides evidence for combination of Cur/Eug with novel H-β-CD-ZNPs formulation have therapeutic potential on the treatment of pregnancy-induced hypertension by rat models.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"118-134"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the efficacy of magnesium alloy fixation screws in a goat femoral condylar fracture model.","authors":"Bin Li, Yong Yang, Liying Sun, Feng Li, Yu Zhang, Wen Tian","doi":"10.1177/08853282251324799","DOIUrl":"10.1177/08853282251324799","url":null,"abstract":"<p><p>This study investigated the efficacy and safety of magnesium alloy screws in repairing small bone fractures using goat lateral femoral condyle fracture models. The animals were randomized into an experimental group receiving magnesium alloy screws (CS/Ф 3.2 × 28 mm, Suzhou Zhuoqia Medical Technology) and a control group receiving titanium alloy screws (CS/Ф 3.2 × 28 mm, Samo Medical Technology Co., Ltd). Postoperative evaluations at 3- and 6-month intervals included assessments of fracture repair, animal health, hematological parameters, histology, and screw degradation. Hematological tests revealed no significant intergroup variations. While gas accumulation near the magnesium screws was noted, the fracture healing outcomes were similar between the magnesium and titanium screw groups, with no deleterious health effects attributed to magnesium screw degradation. Gas liberation during magnesium degradation had no detrimental effect on small fracture recovery. Magnesium screw implementation appears to present no general health risks. Consequently, magnesium alloy could be a promising biomaterial for future fixation screw applications in orthopedics.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"92-104"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143557011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of capecitabine-poly(p-dioxanone) electrospraying nanoparticles and influence of particle size on their colorectal cancer inhibitive efficiency.","authors":"Changlin Huang, Chenwu Yang, Chengmin Feng, Jun Dong, Bing Wang","doi":"10.1177/08853282251320177","DOIUrl":"10.1177/08853282251320177","url":null,"abstract":"<p><p>Colorectal cancer is the fourth leading cause of cancer-related deaths worldwide. Capecitabine is a chemotherapeutic agent commonly used for the treatment of colon cancer. To realize local sustained release, promote efficient local intracellular transport, and mitigate the systemic toxic effects of capecitabine, a capecitabine prodrug, capecitabine-poly (p-dioxanone) (Cap-PPDO), was successfully synthesized. Cap-PPDO was subsequently processed into nanoscale particles with various diameters using electrospraying to investigate the influence of nanoparticle (NP) size on the therapeutic efficiency of Cap-PPDO NPs. Design Expert Software was used to design an experimental scheme for evaluating the influence of electrospraying parameters on NP size and distribution. The in vitro capecitabine release rate of Cap-PPDO NPs was evaluated, and NPs with a size of approximately 300 nm demonstrated the fastest release rate. However, Cap-PPDO NPs with a size of approximately 300 nm exhibited lower proliferation inhibition against SW480 colorectal cancer compared to those with diameters of 200 and 400 nm. To further elucidate the influence of size, the endocytosis of SW480 cells with respect to these differently sized NPs was investigated using flow cytometry and transmission electron microscopy (TEM), given that endocytosis is an important pathway for the intracellular delivery of nanoparticles. The mechanism underlying the size-dependent therapeutic efficiency of Cap-PPDO NPs was ultimately attributed to the size of the mammalian lysosome. Finally, the therapeutic efficacy of Cap-PPDO NPs of various sizes was verified using a nude mouse model of SW480 cell-transplanted tumors.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"135-144"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and antibacterial activity of strontium and silver ion-releasing titanium with high silver treatment concentration.","authors":"Kazuki Orita, Shunsuke Fujibayashi, Yaichiro Okuzu, Seiji Yamaguchi, Koji Goto, Bungo Otsuki, Toshiyuki Kawai, Takayoshi Shimizu, Makoto Hayashi, Norimasa Ikeda, Yusuke Takaoka, Shintaro Honda, Tatsuhito Ikezaki, Shuichi Matsuda","doi":"10.1177/08853282251329276","DOIUrl":"10.1177/08853282251329276","url":null,"abstract":"<p><p>To overcome problems associated with surgical site infection and implant loosening, we developed a titanium (Ti)-based material employing a modified alkaline heat treatment that releases strontium (Sr) and silver (Ag) ions (CaSrAg-Ti). In this study, to determine the optimal Ag treatment concentration, we prepared four different materials-commercially pure Ti (cp-Ti) as a negative control, CaSr1mMAg-Ti, CaSr10mMAg-Ti, and CaSr50mMAg-Ti. Ion release test was performed by immersing the prepared disks in fetal bovine serum. With increased loading of Ag ions, the amount of released ions increased. Colony-forming unit count assay was performed using methicillin-susceptible <i>Staphylococcus aureus</i> and <i>Escherichia coli</i>. High antibacterial activity was observed in CaSr10mMAg-Ti and CaSr50mMAg-Ti groups. <i>In vivo</i> experiments were performed using the rat subcutaneous pocket infection model and evaluated by counting the attached bacteria, wound appearance, and histological evaluation. High antibacterial activity value (AAV >2) and anti-inflammatory effects were observed in the CaSr50mMAg-Ti group. However, CaSr10mMAg-Ti did not exhibit consistent antibacterial activity. For <i>in vivo</i> biocompatibility and bone-bonding ability evaluation, rods were implanted into the rat femur. No cytotoxicity was observed at 1 week, and good bone-bonding ability at 4 and 8 weeks was not significantly different from that of CaSr1mMAg-Ti. To evaluate <i>in vivo</i> bioactivity and cytotoxicity, MC3T3-E1 cells were cultured on disks. CaSr10mMAg-Ti and CaSr50mMAg-Ti significantly inhibited the proliferation and differentiation of MC3T3E1 cells, as well as the production of extracellular matrix <i>in vivo</i>, despite showing good biocompatibility <i>in vivo</i>. In conclusion, CaSr50mMAg-Ti, with increased Ag ion loading, exhibited high antibacterial activity <i>in vivo</i> while maintaining the bone-bonding ability and is a promising therapeutic biomaterial. Further research is needed to determine the optimal combination of therapeutic concentrations of Sr and Ag.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"20-35"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic acid methacryloyl hydrogel with sustained IL-10 release promotes macrophage M2 polarization and motor function after spinal cord injury.","authors":"Zhihua Wang, Denghui Li, Yanghao Wang, Ping Yuan, Wan Zhang, Yihe Zhang, Fei He, Jianyi Yang, Hangchuan Bi, Hao Duan","doi":"10.1177/08853282251329302","DOIUrl":"10.1177/08853282251329302","url":null,"abstract":"<p><p>(1)Background: Inflammation plays a key role in spinal cord injury (SCI), where excessive inflammatory responses exacerbate neural damage and hinder regeneration. Modulating macrophage polarization, particularly through the sustained release of IL-10 to promote the anti-inflammatory M2 phenotype, represents a promising strategy to mitigate inflammation. In this study we developed a Hyaluronic Acid Methacryloyl (HAMA) hydrogel capable of sustained IL-10 release to regulate macrophage polarization and explore its therapeutic potential. (2)Methods: A photo-curable HAMA hydrogel was synthesized via methacrylation and designed for the sustained release of IL-10. The structural and functional properties were characterized using NMR and FT-IR. <i>In vitro</i> assays, including immunofluorescence, flow cytometry, and Western blotting, were performed to evaluate IL-10's effect on macrophage polarization. The anti-inflammatory and reparative effects of the hydrogel were further validated in a rat SCI. (3)Results: The HAMA hydrogel with sustained IL-10 release demonstrated excellent biocompatibility. It significantly promoted macrophage polarization to the anti-inflammatory M2 phenotype by increasing the expression of CD206. In vivo studies demonstrated that the group treated by HAMA with IL-10 exhibited recovery of sensory and motor functions, along with improvement of the inflammatory microenvironment at the site of injury. (4)Conclusion: The HAMA hydrogel with sustained IL-10 release effectively alleviates inflammation, enhances motor function after SCI, and serves as a promising immunomodulatory platform. This novel approach presents considerable potential for improving neural regeneration.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"3-19"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of SF-gel-CS-Hap bionic biphasic porous scaffolds and evaluation of physical, mechanical and biological properties.","authors":"Mingxi Gu, Lin Guo, Changcheng Wang, Fengde Tian, Ruihu Hao","doi":"10.1177/08853282251329591","DOIUrl":"10.1177/08853282251329591","url":null,"abstract":"<p><p><b>Objective:</b> Full-thickness cartilage defect are usually accompanied by subchondral bone damage, which is difficult to self-repair once damaged due to the lack of vascularization and innervation. In this study, a biphasic composite scaffold was developed by combining vacuum freeze-drying and iterative freeze-thawing with gelatin, chitosan, silk fibroin, and hydroxyapatite as the basic materials to explore the feasibility of using them for the repair of total cartilage defects. <b>Methods and Results:</b> Six groups of SF-CS-Gel-nHap porous scaffolds (Hap-0%, Hap-1%, Hap- 2%, Hap-3%, Hap-4%, Hap-5%) were prepared by vacuum freeze-drying and chemical cross-linking using filipin protein (SF), gelatin (Gel), chitosan (CS) and hydroxyapatite (Hap) as the base materials. A series of characterization methods were used to systematically analyze and test the morphological features as well as physical and mechanical properties of the scaffolds. Then a novel bionic biphasic porous scaffold was developed by a combination of freeze-drying and freeze-thawing using the SF-CS-Gel as the cartilage phase and the SF-CS-Gel-2%Hap as the subchondral bone phase. Finally, it was co-cultured with chondrocytes to verify the biological properties of the SF-CS-Gel/SF-CS-Gel-2%Hap bionic biphasic porous composite scaffold in vitro. The results showed that the SF-CS-Gel/SF-CS-Gel-2%Hap biphasic scaffolds had a highly porous mesh structure, with an average pore size of 156.06 ± 42.36 μm in the cartilage phase and 214.38 ± 65.82 μm in the subchondral bone phase. Co-cultured with chondrocytes, the live and dead cells stained, cck-8 growth and proliferation curves showed that the bionic scaffolds had good biocompatibility and cytotoxicity. Cytoskeletal staining showed that the morphology of chondrocytes in the bionic scaffolds could maintain three-dimensional growth as in vivo. <b>Conclusion:</b> The results showed that SF-CS-Gel/SF-CS-Gel-2%Hap biphasic scaffolds have good biocompatibility, biodegradability, stability, appropriate mechanical properties and porosity, and are suitable for repairing articular cartilage and subchondral bone. It is expected to be used as a repair material for articular cartilage in clinical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"61-81"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Bone Regeneration with Silybin-Loaded PCL/Gelatin/Nanoclay Nanocomposite Scaffolds: An In Vitro & In Vivo Study.","authors":"Mohammad Hossein Tayeed, Maryam Tehranchi, Arian Ehterami, Fereshteh Shanei, Ferial Taleghani, Hasan Semyari, Saeed Mahdipour Ganji, Nika Mehrnia, Shaghayegh Bozorgzadeh, Sepehr Zamani, Majid Salehi","doi":"10.1177/08853282251329901","DOIUrl":"10.1177/08853282251329901","url":null,"abstract":"<p><p>This study focuses on the development of a 3-dimensional porous scaffold using Polycaprolactone/Gelatin/Nanoclay (PCL/GNF/NC) for bone tissue engineering. The scaffold incorporates varying dosages of silybin (Sil) through a mixture of electrospinning and thermal-induced phase separation (TIPS) techniques. Assessments of surface shape, porosity, compressive strength, water contact angle, degradation rate, releasing profile, hemolysis, and cell proliferation were among the investigations carried out to appraise the manufactured scaffolds. In vivo evaluation utilized a rat calvaria defect model, with histological analysis employed to assess the results. The scaffolds exhibited porosity within the range of 70-90%, and those containing silybin demonstrated lower compressive strength and contact angle, along with a higher degradation rate compared to those without silybin. Release experiments revealed a 61.09% release of silybin after 28 days. In both in vivo and in vitro assessments, the PCL/GNF/NC/Sil1% scaffold displayed superior cell proliferation and bone healing properties compared to other groups. These findings suggest the potential efficacy of silybin in bone defect treatment, warranting further investigation in future research.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"105-117"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteointegration and remodeling of a novel mineral magnesium phosphate-based bone glue in-vivo.","authors":"Stefanie Hoelscher-Doht, Andrea Ewald, Andreas Fuchs, Paul Otto, Julia Bator, Jan-Peter Grunz, Karl Kneist, Lasse Bögelein, Uwe Gbureck","doi":"10.1177/08853282251326033","DOIUrl":"10.1177/08853282251326033","url":null,"abstract":"<p><p>BackgroundBone adhesives represent a major challenge in the field of materials science. Magnesium phosphate-based mineral bone cements exhibit high biocompatibility, particularly for the use as bone substitutes. In combination with calcium ions and phytic acid as liquid components, these cements demonstrate adhesive properties and potentially represent a promising new type of bone glue. Two of these first cement-adhesives were further investigated for their in vivo properties on distal femoral defects in rabbits in this study.Materials and methodsIn 4.5-month-old New Zealand White rabbits, distal lateral femoral defects in the metaphyseal region were grafted with two different magnesium phosphate-based bone adhesives (MgO, Mg2_,75Ca_0,25(PO₄)₂, phytic acid 22,5/25%wt). After 6 weeks in vivo, the adhesives were evaluated by histologic examination, biomechanical testing, imaging, XRD, and porosimetry measurements for biocompatibility and remodeling to bone.ResultsWhile the bone adhesives showed a higher density on pQCT than the surrounding cancellous bone the biomechanical testing revealed a significantly lower initial stiffness of the adhesives 6 weeks after implantation compared to both the cancellous bone and the cuboid testing before implantation. The decrease in stability was confirmed by the degradation demonstrated histologically as well as by imaging morphology. Remodeling to bone was not yet complete.ConclusionBoth bone adhesives exhibited high biocompatibility without foreign body reactions. Imaging and histology presented a homogeneous picture of degradation of both adhesives and remodeling to bone, which was not yet complete 6 weeks after the implantation.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"82-91"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous oxidized dextran sponges for surgical hemostasis and infection control.","authors":"Hong Xu, Siyuan Li, Peitao Zhuang","doi":"10.1177/08853282251329202","DOIUrl":"10.1177/08853282251329202","url":null,"abstract":"<p><p>Surgical procedures frequently result in varying degrees of bleeding and infection, which can impede patient recovery, particularly in situations of limited blood supply. Minimizing surgical blood loss and preventing infections remain crucial clinical challenges. To address these tissues, we developed a porous hemostatic sponge by aldehyde-functionalizing dextran. The high porosity and blood absorption capacity of ODex sponges enables them to effectively concentrate red blood cells, platelets, and coagulation factors, forming a blood clot together with the sponge matrix. The aldehyde groups bind to the amines in the tissue, helping to seal the bleeding site. This innovation significantly reduced clotting times in both in <i>vitro</i> and in <i>vivo</i> experiments. Furthermore, the sponge demonstrated excellent biocompatibility and potent antimicrobial activity. These findings highlight oxidized dextran as a highly promising hemostatic biomaterial with strong antimicrobial capabilities, offering the potential for broad clinical applications.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"50-60"},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylene blue functionalized ZnO nanoparticles: A promising approach for photodynamic therapy in the treatment of leishmaniasis.","authors":"Thainara Alves Gouvea, Jessica Aparecida Ribeiro Ambrósio, Janicy Arantes Carvalho, Vitor Luca Moura Marmo, Luciana Maria Cortez Marcolino, Juliana Guerra Pinto, Juliana Ferreira-Strixino, Andreza Ribeiro Simioni, Erika Peterson Gonçalves","doi":"10.1177/08853282251350317","DOIUrl":"https://doi.org/10.1177/08853282251350317","url":null,"abstract":"<p><p>Zinc oxide (ZnO) has wide application in engineering, but its use in medical sciences has aroused growing interest. In this context, ZnO nanoparticles were investigated as vehicles for the delivery of methylene blue (MB), a photosensitizer (PS) used in photodynamic therapy (PDT) against <i>Leishmania braziliensis</i>. ZnO-NPs were produced by a coprecipitation method and characterized by several techniques, including scanning electron microscopy (SEM), UV-VIS spectroscopy, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The results showed that the ZnO-NPs presented uniform spheroidal morphology with open porosity, allowing an efficient methylene blue (MB) encapsulation without significant structural changes, ensuring stability and the absence of aggregation. The PS was adsorbed on the porous surface of the ZnO nanoparticles, characterized by scanning electron microscopy (SEM) and steady-state analysis techniques. Spectroscopic analysis confirmed the maintenance of the photosensitizing properties of MB. The biological activity was evaluated in vitro using the trypan blue exclusion method in macrophages infected with <i>Leishmania braziliensis</i>. After loading with the photosensitizer, they maintained their photophysical properties, ensuring the proper location of the dye within the cells. In vitro assays demonstrated the internalization of ZnO/MB-NPs by infected macrophages and a significant reduction in parasite viability after light activation. Thus, the results showed that the developed system exhibits a promising photodynamic activity with relevant therapeutic potential in treating macrophages infected by <i>Leishmania braziliensis</i>.</p>","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251350317"},"PeriodicalIF":2.3,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}