Journal of Biomaterials Applications最新文献_第8页

Preparation and application of nanosilver/nanocellulose composite antimicrobial strain-responsive dual network hydrogels. 纳米银/纳米纤维素复合抗菌双网水凝胶的制备及应用。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-27 DOI: 10.1177/08853282251340167

Qinwen Wang, Zhuoyan Xie, Aimin Tang, Wenjin Chen, Yingyao Liu

{"title":"Preparation and application of nanosilver/nanocellulose composite antimicrobial strain-responsive dual network hydrogels.","authors":"Qinwen Wang, Zhuoyan Xie, Aimin Tang, Wenjin Chen, Yingyao Liu","doi":"10.1177/08853282251340167","DOIUrl":"https://doi.org/10.1177/08853282251340167","url":null,"abstract":"With the progress of science and technology, smart wearable devices prepared based on antimicrobial conductive hydrogels have come to have important applications in motion detection, medical monitoring, human-machine interface and soft robotics. On the basis of satisfying the performance of antimicrobial conductive, hydrogels also need to improve the mechanical properties to adapt to more wearable device applications. In this study, glycerol and agar were introduced on the basis of nanosilver/nanocellulose composite antimicrobial strain-responsive hydrogels (AP hydrogels), and nanosilver/nanocellulose composite antimicrobial strain-responsive dual-network hydrogel (APA-DN hydrogel) could be constructed by a two-step moulding method of thermal initiation and sol-gelation, and encapsulated into a strain-responsive sensor. Tensile fracture strain and stress of the APA-DN hydrogel could reach that concomitant with an elongation of 2182.0 mm. The tensile fracture strain and stress of the APA-DN hydrogel can reach 2182.71% and 279.76 kPa, and the modulus of elasticity and toughness can reach 36.35 kPa and 2772.98 kJ/m3, thereby realising enhanced mechanical properties on the basis of the AP hydrogel. The relative resistance of the APA-DN hydrogel sensors was stable in the range of 0-120% under 100% strain cycling, maintaining stable repeatability and durability of strain response. The APA-DN hydrogels are capable of outputting stable and reproducible electrical signals in the monitoring of hand and head movements, and they are expected to be applied in human behaviour detection by collecting and classifying the response signals in the future.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251340167"},"PeriodicalIF":2.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human osteoblast response to uncemented knee implant surface structures and osteoclast formation in vitro. 人成骨细胞对非骨水泥膝关节假体表面结构和破骨细胞形成的反应。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-25 DOI: 10.1177/08853282251346324

Raymond Puijk, Behrouz Zandieh-Doulabi, Wendy J A M Runderkamp, Bart G Pijls, Jenneke Klein-Nulend, Peter A Nolte

{"title":"Human osteoblast response to uncemented knee implant surface structures and osteoclast formation in vitro.","authors":"Raymond Puijk, Behrouz Zandieh-Doulabi, Wendy J A M Runderkamp, Bart G Pijls, Jenneke Klein-Nulend, Peter A Nolte","doi":"10.1177/08853282251346324","DOIUrl":"https://doi.org/10.1177/08853282251346324","url":null,"abstract":"Early bone ingrowth and minimal resorption ensure rigid fixation in uncemented total knee replacements. Trabecular titanium-aluminum-vanadium (Ti6Al4V) and hydroxyapatite (HA)-coated vacuum-plasma-sprayed (VPS) titanium with varying porosities and HA-coating thicknesses, have been developed to enhance fixation, though bone cellular response remains largely unknown. This study evaluated osteoblast responses to trabecular Ti6Al4V and three VPS titanium surfaces with 20%-40% or 30%-70% porosity and HA coatings of 60, 80, or 90 µm. Human primary osteoblasts were seeded and cultured for 29 days, to assess seeding efficiency, viability, metabolic activity, alkaline phosphatase activity, and the effect of osteoblast-released factors in conditioned medium during the last 4 days of culture on osteoclast formation. VPS-HA groups were first compared individually; when no differences were found, data were pooled for comparison with the trabecular group. Osteoblast seeding efficiency, viability, metabolic activity, and alkaline phosphatase activity were similar between VPS-HA surfaces. Moreover, osteoblast-conditioned medium did not affect osteoclast formation. Osteoblast seeding efficiency and viability were similar between the pooled VPS-HA and trabecular surface. Compared to the pooled VPS-HA, the trabecular surface increased osteoblast metabolic (1.5-2.7-fold) and alkaline phosphatase activity (6.5-15.2-fold). Osteoblast-conditioned medium reduced osteoclast formation (2.1-3.4-fold) on trabecular compared to the pooled VPS-HA surface. In conclusion, these findings show that VPS-HA surfaces with various porosities and HA-coating thicknesses similarly affect osteoblast and osteoclast responses, while trabecular surfaces enhance osteoblast responsiveness and inhibit osteoclast formation. These results might help to further improve early stability and reduce long-term loosening risk in uncemented knee replacements.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"8853282251346324"},"PeriodicalIF":2.3,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

pH-triggered small molecule nanodrugs self-assembled from tryptamine-cinnamaldehyde and fisetin for targeted sepsis-associated encephalopathy therapy. 由色胺-肉桂醛和非瑟酮自组装的ph触发小分子纳米药物用于靶向败血症相关脑病治疗。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-02 DOI: 10.1177/08853282251318052

Ximing Deng, Jinyao Zhou, Wei Fang, Rao Sun, Guoqing Yan, Yun Sun

{"title":"pH-triggered small molecule nanodrugs self-assembled from tryptamine-cinnamaldehyde and fisetin for targeted sepsis-associated encephalopathy therapy.","authors":"Ximing Deng, Jinyao Zhou, Wei Fang, Rao Sun, Guoqing Yan, Yun Sun","doi":"10.1177/08853282251318052","DOIUrl":"10.1177/08853282251318052","url":null,"abstract":"Sepsis-associated encephalopathy (SAE) is an acute diffuse brain dysfunction, but its clinical treatment just focuses on antibiotics and supportive therapy, which fail to directly limit the development of SAE. Herein, this work highlights the development of pH-triggered small molecule nanodrugs self-assembled from tryptamine (Try)-cinnamaldehyde (CA) and fisetin for targeted SAE therapy. The imine linkage in Try-CA and acid-dependent protonation of Try and fisetin endow the nanodrugs with pH-triggered dynamic changes of particle sizes, surficial charges, and drug release. Moreover, the combined use of Try-CA and fisetin also endows the nanodrugs with superior antioxidative, anti-inflammatory and antibacterial capabilities compared to their individual use. These characteristics of the nanodrugs facilitate long-term circulation stability, effective penetration through BBB, selective accumulation in the brain, and target to central and peripheral focal areas, thereby achieving comprehensive treatment or relief of SAE. Thus, these attractive experimental results illuminate the enormous potential of such pH-triggered small molecule nanodrugs for targeted SAE therapy, advancing their use in clinics.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1165-1176"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143079191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peptides-functionalized gold nanostars enhanced degradation of PD-L1 for improved prostate cancer immunotherapy. 肽功能化金纳米星增强PD-L1降解，改善前列腺癌免疫治疗。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-24 DOI: 10.1177/08853282251319473

Liangjun Tao, Yifei Zhang, Jingwei Zhang, Jianping Tao, Yu Gong, Jun Mao, Qixing Tian, Ping Ao, Dong Zhuo

{"title":"Peptides-functionalized gold nanostars enhanced degradation of PD-L1 for improved prostate cancer immunotherapy.","authors":"Liangjun Tao, Yifei Zhang, Jingwei Zhang, Jianping Tao, Yu Gong, Jun Mao, Qixing Tian, Ping Ao, Dong Zhuo","doi":"10.1177/08853282251319473","DOIUrl":"10.1177/08853282251319473","url":null,"abstract":"Blockage of the interaction between programmed death receptor-1 (PD-1) and programmed death ligand-1 (PD-L1) can restore T-cell activity and enhance antitumor immunity. PD-1/PD-L1 pathway inhibitors have promising applications in the treatment of advanced prostate cancer (PCa). We successfully developed a peptides-functionalized gold nanoconstruct (P-AuNS) consisted of PD-L1-binding peptide (PD-L1pep, P) and gold nanostar (AuNS), which could bind to cell-surface PD-L1 specifically and deliver PD-L1 into PCa cells with high efficiency. In PCa cells, P-AuNS can efficiently degrade PD-L1 in a lysosomal-dependent manner. In the co-culture system of Jurkat cells and DU145 cells, P-AuNS restored the proliferative capacity and interferon-gamma (IFN-γ) secretion level of Jurkat cells inhibited by co-cultured DU145 cells, indicating that P-AuNS effectively hampered the interaction between PD-1 and PD-L1. In addition, in PCa-bearing mice, P-AuNS can effectively inhibit tumor growth and down-regulate PD-L1 protein levels, and in vivo experimental results show that P-AuNS has no systemic toxicity. P-AuNS block the interaction between PD-1 and PD-L1 by efficiently degrading PD-L1, thus restoring the antitumor activity of T cells and inhibiting tumor progression of PCa. In all, P-AuNS has great promise as a potential immunotherapy strategy in the treatment of advanced PCa and even other solid tumors.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1188-1201"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High α-lipoic acid-loaded hollow mesoporous prussian blue nanozymes for targeted therapy of nasopharyngeal carcinoma in mice. 高α-硫辛酸负载的中空介孔普鲁士蓝纳米酶靶向治疗小鼠鼻咽癌。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-05 DOI: 10.1177/08853282251318514

Ya Pan, Xiaofeng Wang, Xuejun Zhou, Haipeng Chen, Yuxia Zou

{"title":"High α-lipoic acid-loaded hollow mesoporous prussian blue nanozymes for targeted therapy of nasopharyngeal carcinoma in mice.","authors":"Ya Pan, Xiaofeng Wang, Xuejun Zhou, Haipeng Chen, Yuxia Zou","doi":"10.1177/08853282251318514","DOIUrl":"10.1177/08853282251318514","url":null,"abstract":"This study successfully constructs a tumor-targeting α-lipoic acid-loaded hollow mesoporous prussian blue nanozyme (AHPRzyme) for targeted therapy of nasopharyngeal carcinoma in mice. In these nanozymes, Arg-Gly-Asp (RGD) acts as a targeting ligand, enabling effective targeting of tumor cells. Additionally, AHPRzyme exhibits multiple anti-tumor mechanisms: ① The prussian blue nanozymes in AHPRzyme have catalase (CAT) activity, which decomposes H2O2 in human nasopharyngeal carcinoma CEN2 cells into non-toxic H2O, reducing H2O2 levels and minimizing damage to normal cells. The released O2 helps alleviate the hypoxic environment of the tumor, inhibiting lactate production due to hypoxia and consequently suppressing tumor growth. ② The prussian blue nanozymes also have peroxidase (POD) activity, which catalyzes H2O2 in tumor cells to generate ·OH, a reactive oxygen species, leading to tumor cell apoptosis. ③ The α-lipoic acid structure in AHPRzyme contains disulfide bonds that react with GSH, depleting excess glutathione (GSH) in tumor cells, disrupting the oxidative stress balance within the cells, and making them more sensitive to reactive oxygen species, thereby increasing tumor cell apoptosis. In summary, AHPRzyme can inhibit tumor cell growth and promote tumor cell apoptosis by improving the tumor microenvironment, achieving the goal of anti-nasopharyngeal carcinoma therapy.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1177-1187"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143189525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research progress on intervertebral disc repair strategies and mechanisms based on hydrogel. 基于水凝胶的椎间盘修复策略及机制研究进展。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-10 DOI: 10.1177/08853282251320227

Zekun Hua, Yinuo Zhao, Meng Zhang, Yanqin Wang, Haoyu Feng, Xiaochun Wei, Xiaogang Wu, Weiyi Chen, Yanru Xue

{"title":"Research progress on intervertebral disc repair strategies and mechanisms based on hydrogel.","authors":"Zekun Hua, Yinuo Zhao, Meng Zhang, Yanqin Wang, Haoyu Feng, Xiaochun Wei, Xiaogang Wu, Weiyi Chen, Yanru Xue","doi":"10.1177/08853282251320227","DOIUrl":"10.1177/08853282251320227","url":null,"abstract":"Intervertebral disc degeneration (IDD) arises from a complex interplay of genetic, environmental, and age-related factors, culminating in a spectrum of low back pain (LBP) disorders that exert significant societal and economic impact. The present therapeutic landscape for IDD poses formidable clinical hurdles, necessitating the exploration of innovative treatment modalities. The hydrogel, as a biomaterial, exhibits superior biocompatibility compared to other biomaterials such as bioceramics and bio-metal materials. It also demonstrates mechanical properties closer to those of natural intervertebral discs (IVDs) and favorable biodegradability conducive to IVD regeneration. Therefore, it has emerged as a promising candidate material in the field of regenerative medicine and tissue engineering for treating IDD. Hydrogels have made significant strides in the field of IDD treatment. Particularly, injectable hydrogels not only provide mechanical support but also enable controlled release of bioactive molecules, playing a crucial role in mitigating inflammation and promoting extracellular matrix (ECM) regeneration. Furthermore, the ability of injectable hydrogels to achieve minimally invasive implantation helps minimize tissue damage. This article initially provides a concise exposition of the structure and function of IVD, the progression of IDD, and delineates extant clinical interventions for IDD. Subsequently, it categorizes hydrogels, encapsulates recent advancements in biomaterials and cellular therapies, and delves into the mechanisms through which hydrogels foster disc regeneration. Ultimately, the article deliberates on the prospects and challenges attendant to hydrogel therapy for IDD.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1121-1142"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Injectable ADM temperature-sensitive hydrogel loaded with bFGF in diabetic rat wound healing study. 载bFGF的ADM温敏水凝胶在糖尿病大鼠伤口愈合中的应用研究。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-18 DOI: 10.1177/08853282251321943

Haojiao Chen, Jianping Sun, Wenyang Liu

{"title":"Injectable ADM temperature-sensitive hydrogel loaded with bFGF in diabetic rat wound healing study.","authors":"Haojiao Chen, Jianping Sun, Wenyang Liu","doi":"10.1177/08853282251321943","DOIUrl":"10.1177/08853282251321943","url":null,"abstract":"Background: Diabetic wound is one of the most common diabetic chronic complications. Effective treatments of diabetic wound remain limited. Here, we explored the effects of basic fibroblast growth factor (bFGF)-acellular dermal matrix (ADM) hydrogel on the diabetic wound. Methods: The bFGF-ADM hydrogel was manufactured by mixing 180 µL ADM hydrogel and 20 µL bFGF aqueous solution (10 mg/mL). The morphology of ADM hydrogel and bFGF-ADM hydrogel was observed under scanning electron microscope. The release property of bFGF-ADM hydrogel was determined by ELISA. CCK-8 assay was utilized to estimate the cell viability of mouse skin fibroblasts. The diabetes mellitus (DM) model was established in rats. The four wounds on the back of each DM rat were treated with the ADM hydrogel, bFGF-ADM hydrogel, bFGF aqueous solution and no solution (control), respectively. The wound healing rate of each rat was estimated. The traumatized skin tissue of each rat was observed by H&E staining and Sirius red staining. Results: The bFGF-ADM hydrogel displayed an interconnected pore structure and bFGF was gradually released from the bFGF-ADM hydrogel over time. The bFGF-ADM hydrogel could enhance the cell viability of skin fibroblasts and promote the wound healing rate, the re-epithelialization of wound and increase the collagen fiber content of dermis. And the bFGF-ADM hydrogel exhibited better therapeutic effects of diabetic wound than either bFGF or ADM alone. Conclusions: Our study revealed that the bFGF-ADM hydrogel could promote diabetic wound healing.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1156-1164"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143449119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dexamethasone loaded blend poly hydroxybutyrate-stearic acid nanoparticles for ocular inflammation. 地塞米松负载的混合聚羟基丁酸-硬脂酸纳米颗粒用于眼部炎症。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-27 DOI: 10.1177/08853282251324619

Ebru Erdal, Nagihan Uğurlu, Esin Akbay Çetin, Nelisa Laçin Türkoğlu, Cafer Çakal, Murat Demirbilek

{"title":"Dexamethasone loaded blend poly hydroxybutyrate-stearic acid nanoparticles for ocular inflammation.","authors":"Ebru Erdal, Nagihan Uğurlu, Esin Akbay Çetin, Nelisa Laçin Türkoğlu, Cafer Çakal, Murat Demirbilek","doi":"10.1177/08853282251324619","DOIUrl":"10.1177/08853282251324619","url":null,"abstract":"The aim of this study is to develop and analyze dexamethasone-loaded poly hydroxybutyrate-stearic acid blend nanoparticles for the treatment of non-bacterial uveitis. Uveitis is a chronic inflammatory eye disease responsible for 10-15% of global blindness. While repeated intravitreal steroid injection is a successful treatment strategy, it has drawbacks such as cataracts and retinal detachment. Serious side effects and high costs caused by recurrent infections are important limiting factors. Controlled release systems are preferred to maintain therapeutic drug concentrations in the vitreous humor while minimizing the frequency of injections. In this study, PHB-stearic acid blend nanoparticles with varying ratios of dexamethasone suitable for intravitreal injection were fabricated. It was assessed the effects of the stearic acid ratio on loading efficiency, release profiles, size distribution, and thermal properties. The results indicated that an increase in the stearic acid ratio led to a reduction in nanoparticle diameter and an enhancement in the dexamethasone loading capacity. Additionally, higher stearic acid ratios resulted in a decrease in the melting enthalpy of the nanoparticles, which expedited dexamethasone release. Importantly, the nanoparticle formulations exhibited no toxicity to eye endothelial cells or THP-1 cells.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1211-1221"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and characterization of dual drug loaded magnetic gold nanohybrid system for osteoarthritis. 骨关节炎双药负载磁性金纳米杂化系统的研制与表征。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-03-03 DOI: 10.1177/08853282251324352

Gul Rehman, Jadoon Khan, May M Alrashed, Muhammad Abbas, Masood Ur Rehman, Tawaf Ali Shah, Kotb A Attia, Arif Ahmed Mohammed

{"title":"Development and characterization of dual drug loaded magnetic gold nanohybrid system for osteoarthritis.","authors":"Gul Rehman, Jadoon Khan, May M Alrashed, Muhammad Abbas, Masood Ur Rehman, Tawaf Ali Shah, Kotb A Attia, Arif Ahmed Mohammed","doi":"10.1177/08853282251324352","DOIUrl":"10.1177/08853282251324352","url":null,"abstract":"Cartilage deterioration in patients with osteoarthritis presents a significant challenge, primarily attributable to the inadequate oral bioavailability and poor dosage compliance of chondroprotective agents. The Chondroitin Sulphate (CS) is a stabilizing and reducing agent for metal NP as well as homing agent by binding to surface molecules (CD44, L-selectin, P-selectin, and annexin-6) of chondrocytes at the OA site. This study was designed to develop intra-articular magnetic gold nanohybrids for the co-delivery of chondroitin sulfate, glucosamine sulfate, and gold, aiming to achieve synergistic anti-inflammatory and cartilage regenerative effects and in vitro assessments of drug release were conducted. Additionally, in animal study, the male albino rats underwent anesthesia by inhaling isoflurane using the open-drop exposure method, and chondrocytes were then harvested for cytotoxicity and biocompatibility assays. Physical characterization revealed 66 nm particle size with uniform distribution and colloidal stability of MGN-CS-GS. Zeta potential and FTIR analysis showed electrostatic interaction between the carboxyl and amino groups of MGN-CS and GS. VSM and EDX confirmed paramagnetic and core-shell characteristics of nanohybrids, respectively. It was found that the MGN-CS-GS released more CS (72%) and GS (85%) at acidic pH with continuous release pattern, which will improve patient compliance. The nanohybrid's cytotoxicity assay demonstrated excellent biocompatibility and cellular viability of OA chondrocytes triggered by interleukin-1β (IL-1β) compared to marketed formulation. The results demonstrated that MGN-CS-GS continuously released both drugs with high biocompatibility and cellular viability of OA chondrocytes. The successful synthesis of MGN-CS-GS is a foundation for further research on its potential application as a novel co-drug carrier nanohybrid system.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1222-1239"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143542083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stimulation of macrophage cell lines confined with silica and/or silicon particles and embedded in structured collagen gels. 用二氧化硅和/或硅颗粒封闭并包埋在结构化胶原凝胶中的巨噬细胞系的刺激。

IF 2.3 4区医学

Journal of Biomaterials Applications Pub Date : 2025-05-01 Epub Date: 2025-02-11 DOI: 10.1177/08853282251319875

Pedro U Muñoz-González, Jorge Delgado, Gerardo González-García, Birzabith Mendoza-Novelo

{"title":"Stimulation of macrophage cell lines confined with silica and/or silicon particles and embedded in structured collagen gels.","authors":"Pedro U Muñoz-González, Jorge Delgado, Gerardo González-García, Birzabith Mendoza-Novelo","doi":"10.1177/08853282251319875","DOIUrl":"10.1177/08853282251319875","url":null,"abstract":"Macrophages encapsulated in composite gels are subjected to a three-dimensional (3D) microenvironment and material-related stimuli that allow modulation of their phenotypes. Herein, 3D collagen fibrillar networks structured with di- or tri-functionalized oligourethanes, including Si-O or Si-Si particles confined therein, are compared regarding their physicochemical properties and material-guided macrophage activation. Gelation kinetics, degradation/swelling, and rheometric results demonstrated that the properties of the composite gels depend on the oligourethane functionalization number (derived from diols/triols and L-Lysine diisocyanate, LDI) and silica incorporation. Human or murine macrophages seeded or encapsulated in the composite gels showed good viability and the adoption of an anti-inflammatory phenotype in response to the silica in the composite gel, showing accelerated gelation when cell culture components are present in the liquid precursors. An increase in cell viability proportional to the storage modulus was observed. ELISA tests strongly suggest that the Si-Si nanoparticles in the composites can antagonize the pro-inflammatory stimulation with lipopolysaccharides (LPS) and interferon-gamma (IFNγ), even promoting an anti-inflammatory response in embedded cells after 24 h. Silicon-doped and crosslinked collagen gels have good potential to modulate macrophage inflammatory response, serving as a 3D immunomodulatory scaffold.","PeriodicalId":15138,"journal":{"name":"Journal of Biomaterials Applications","volume":" ","pages":"1240-1257"},"PeriodicalIF":2.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0