Genipin stabilized fibrin microbeads: Carrying cytokines to form niches for stem cell differentiation.

IF 2.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Journal of Biomaterials Applications Pub Date : 2025-05-20 DOI:10.1177/08853282251344394

Qian Chen, Pingping Hu, Wenjing Yang, Xiuquan Xu, Genbao Shao

引用次数: 0

Abstract

Niches, which are combinations of extracellular matrix and cytokines, play essential roles in the stem cell biology. In this study, genipin stabilized fibrin microbeads (gFMBs) were prepared through oil emulsion method. Then, sonic hedgehog (SHH) was crosslinked to the surface of gFMBs by using genipin. These gFMBs were designated as gFMB@SHH since SHH was attached to their surface. Moreover, ectomesenchymal stem cells (EMSCs) were cultured, characterized, and used to test gFMB@SHH. Genipin not only changed the color of fibrin microbeads (FMBs) to deep blue, but also stabilized FMBs by delaying their degradation in vitro. In addition to the nontoxic and proliferation promoting effects of gFMB@SHH on EMSCs, gFMBs@SHH could induce neural differentiation of EMSCs by stimulating the SHH/Gli pathway. Therefore, genipin stabilized fibrin microbeads might be a promising structure to construct niches for in vitro stem cell researches.

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Genipin稳定纤维蛋白微珠：携带细胞因子形成干细胞分化的壁龛。

小生境是细胞外基质和细胞因子的组合，在干细胞生物学中起着重要的作用。本研究采用油乳法制备了格尼平稳定纤维蛋白微珠（gfmb）。然后，用genipin将sonic hedgehog基因（SHH）交联到gfmb表面。这些gfmb被命名为gFMB@SHH，因为SHH附着在它们的表面。此外，外充质干细胞（EMSCs）被培养、表征并用于gFMB@SHH测试。Genipin不仅使纤维蛋白微珠（fibrin microbeads, fmb）的颜色变为深蓝色，而且通过延缓其体外降解来稳定fmb。除了gFMB@SHH对EMSCs具有无毒和促进增殖的作用外，gFMBs@SHH还可以通过刺激SHH/Gli通路诱导EMSCs的神经分化。因此，格尼平稳定的纤维蛋白微球可能是一种很有前景的体外干细胞研究结构。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biomaterials Applications 工程技术-材料科学：生物材料

CiteScore

5.10

自引率

3.40%

发文量

144

审稿时长

1.5 months

期刊介绍： The Journal of Biomaterials Applications is a fully peer reviewed international journal that publishes original research and review articles that emphasize the development, manufacture and clinical applications of biomaterials. Peer-reviewed articles by biomedical specialists from around the world cover: New developments in biomaterials, R&D, properties and performance, evaluation and applications Applications in biomedical materials and devices - from sutures and wound dressings to biosensors and cardiovascular devices Current findings in biological compatibility/incompatibility of biomaterials The Journal of Biomaterials Applications publishes original articles that emphasize the development, manufacture and clinical applications of biomaterials. Biomaterials continue to be one of the most rapidly growing areas of research in plastics today and certainly one of the biggest technical challenges, since biomaterial performance is dependent on polymer compatibility with the aggressive biological environment. The Journal cuts across disciplines and focuses on medical research and topics that present the broadest view of practical applications of biomaterials in actual clinical use. The Journal of Biomaterial Applications is devoted to new and emerging biomaterials technologies, particularly focusing on the many applications which are under development at industrial biomedical and polymer research facilities, as well as the ongoing activities in academic, medical and applied clinical uses of devices.