Dentistry Journal最新文献

{"title":"Flexural Stiffness and Force Retention of Six 3D-Printed Thermoplastics for Orthodontic Appliances: An In Vitro Screening Study.","authors":"Marco Serafin, Marina Borgese, Elisa Boccalari, Gilberto Binda, Mario Raspanti, Piero Antonio Zecca","doi":"10.3390/dj14090565","DOIUrl":"10.3390/dj14090565","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Directly printed orthodontic appliances rely mostly on vat-photopolymerisation resins; pre-polymerised thermoplastics processed by fused deposition modelling (FDM) and selective laser sintering (SLS) are a less-explored, single-component route, but are poorly characterised. This study compared six thermoplastic material-process systems, supplied for biomedical or dental use, using a single intraoral-simulating protocol. <b>Methods:</b> Thin specimens (25 × 5 × 0.6 mm) of polyethylene terephthalate glycol (PETG), polycarbonate (PC), polyamide (PA), polyether-ether-ketone (PEEK) and a thermoplastic copolyester elastomer (TPC) printed by FDM, and of polyamide 12 (PA12) by SLS, underwent three-point bending and 10 min stress relaxation at 37 °C in artificial saliva (<i>n</i> = 5). Differences were assessed using the Kruskal-Wallis test with Dunn post hoc comparisons (Holm correction), and Fourier-transform infrared (FTIR) spectroscopy was used to compare virgin and printed materials for the five FDM polymers. <b>Results:</b> Apparent flexural moduli were 989 (PA12), 750 (PC), 687 (PEEK), 509 (PETG), 85 (PA) and 32 MPa (TPC), a very large material effect (ε² = 0.95), with post hoc comparisons separating only the extremes of that range. Relaxation over 10 min ranged from 2.6% (PC) to 22.1% (PA12) among the five materials in which it could be quantified, with TPC falling below the load-cell resolution; expressed as force, PA12 lost the most (0.86 N) and yet still delivered 3.01 N, which was above the initial force of PEEK, PETG, PA and TPC, though not separable from that of PC, which retained 97.4% of its own. Virgin and printed spectra were concordant (Pearson r ≥ 0.986, against ≤ 0.80 between different materials). <b>Conclusions:</b> The PA12/SLS system was the stiffest, followed by PC and PEEK, but, as the only laser-sintered material, its polymer and process effects cannot be separated. PETG and PC combine useful stiffness with accessible FDM; PA and TPC are far more compliant. These specimen-level findings provide a screening basis for selecting material-process systems for printed orthodontic appliances, preceding device-level validation.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605013/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of Dual-Light Photodynamic Therapy on Oral Health in Adolescents Undergoing Fixed Orthodontic Treatment: A Randomized, Assessor-Blinded and Controlled Trial.","authors":"Katja Hashemi Elses, Tommi Pätilä, Ann-Marie Roos Jansåker","doi":"10.3390/dj14090564","DOIUrl":"10.3390/dj14090564","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Patients with fixed orthodontic appliances are at increased risk of plaque accumulation, gingivitis, and caries because brackets and bands hinder mechanical hygiene. This study evaluated whether regular home use of dual-light antibacterial photodynamic therapy (aPDT) supports oral hygiene during fixed orthodontic treatment in adolescents. <b>Methods</b>: In a single-center, randomized, assessor-blinded trial, forty-one adolescents starting fixed orthodontic treatment were randomised to a Control group (standard hygiene) or a Test group (standard hygiene plus home-use dual-light aPDT). Visible Plaque Index (VPI%), Bleeding on Probing (BOP%), and Orthodontic Plaque Index (OPI) were assessed at baseline and 12 weeks by an assessor blinded to allocation. After two protocol-driven exclusions, 39 participants (20 Test group, 19 Control group) were analysed. Site-level proportions were compared using the chi-square test. <b>Results</b>: VPI decreased in both groups, with a larger mean within-group reduction in the Test group (-7.0%) than in the Control group (-3.7%); exploratory site-level analysis showed a greater reduction in plaque-positive sites in the Test group at 12 weeks (<i>p</i> < 0.001). BOP increased in both groups but to a smaller extent in the Test group (mean +2.4% versus +5.7%), and a higher proportion of Test-group participants maintained BOP ≤ 10% (85% versus 58%); bleeding-positive sites were lower in the Test group at 12 weeks (<i>p</i> < 0.001). The Test group also showed a flatter distribution of OPI scores around orthodontic brackets. No device- or protocol-related adverse events were reported. <b>Conclusions</b>: In adolescents undergoing fixed orthodontic treatment, regular home use of dual-light aPDT was safe and associated with better site-level plaque and bleeding control, with directional but non-significant trends at the participant level. The findings support further evaluation of dual-light aPDT as an adjunct to mechanical hygiene during this vulnerable treatment period.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605755/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optical Attenuation Analysis of Biofilm Formation on Dental Prostheses Using Time-Domain Optical Coherence Tomography and Atomic Force Microscopy.","authors":"Maria Cecilia Galvez, Aaron Mharl Barlisan, Emmanuelle Hermosilla, Mark Nickole Tabafa, Ernest Macalalad, Jose Esmeria, Edgar Vallar, Seiroh Okaneya, Jumar Cadondon, Tatsuo Shiina","doi":"10.3390/dj14090563","DOIUrl":"10.3390/dj14090563","url":null,"abstract":"<p><p><b>Background/Objectives:</b> Dental biofilm formation on prosthetic materials contributes to oral diseases and material degradation. This pilot study evaluated the feasibility of combining time-domain optical coherence tomography (TD-OCT) and non-contact atomic force microscopy (NC-AFM) to characterize biofilm-associated optical and surface changes in dental prosthesis materials following antiseptic treatment. <b>Methods:</b> Acrylic resin, composite resin, and stainless-steel specimens were inoculated with <i>Streptococcus mutans</i> biofilms and exposed to water, Orahex, and Watsons mouthwash. TD-OCT was used to obtain extinction coefficient (EC) and optical thickness measurements, while NC-AFM was performed on Orahex-treated specimens to assess nanoscale surface morphology. EC distributions were analyzed descriptively from valid TD-OCT A-scans. <b>Results:</b> Within the examined specimens, EC distributions varied following biofilm formation and antiseptic treatment, with material-dependent responses. Composite resin exhibited the clearest reduction in EC following Watsons treatment, whereas acrylic resin and stainless-steel specimens showed more variable responses. Physical thickness remained relatively stable, while NC-AFM observations of the Orahex-treated specimens supported the TD-OCT findings by demonstrating corresponding surface morphological changes. <b>Conclusions:</b> This pilot study demonstrates the preliminary feasibility of integrating TD-OCT with NC-AFM for non-destructive evaluation of biofilm-associated optical and surface changes in dental prosthesis materials. The findings are descriptive and exploratory, highlighting the potential of TD-OCT for monitoring prosthetic materials while emphasizing the need for future studies incorporating independent biological replicates and complementary biological validation techniques.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605691/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149037052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening 'Gum Disease or Problem' Status Using Molar Probing Depths: Model Development and Validation in NHANES Young to Middle-Aged Adults.","authors":"Miika Penttala","doi":"10.3390/dj14090560","DOIUrl":"10.3390/dj14090560","url":null,"abstract":"<p><p><b>Background/Objectives:</b> A parsimonious multivariable model was engineered and externally validated using dental electronic health records to screen for a clinician-evaluated \"gum disease or related problem\" status. Designed for seamless electronic integration, a positive screening result triggers a targeted referral-and the model is well-suited for dental hygienist use- while providing clinical decision support for dentists during definitive diagnoses. This automated support standardises workflows, maximises team efficiency, and supports data-driven clinical decision-making. <b>Methods:</b> This cross-sectional study developed the model using the US National Health and Nutrition Examination Survey (NHANES) 2011-2012 cohort (<i>n</i> = 1732) and independently validated it on the 2013-2014 replication cycle (<i>n</i> = 1855) for the high-risk 30-50-year-old demographic. Survey-weighted logistic regression incorporated age, gender, and eight targeted molar probing depth (PD) measurements paired with binary indicators for tooth presence to predict whether an individual has a gum disease or related problem. <b>Results:</b> External validation demonstrated high parameter generalisability, yielding a pooled population-level AUC of 0.859 (95% CI: 0.828-0.885). At the prevalence-matched threshold of <i>Pt</i> = 0.367, the framework yielded a sensitivity of 78.39% (95% CI: 69.48-85.29%) and a specificity of 79.10% (95% CI: 74.55-83.04%). Demonstrating public health utility via Decision Curve Analysis, the model avoided approximately 39 unnecessary clinical examinations by a dentist per 100 individuals at a practical clinical decision threshold of 0.33, achieving a substantial pooled net benefit compared with a treat-all strategy without compromising case-detection metrics. <b>Conclusions:</b> This scalable screening model offers a highly practical pathway for gum disease and related problem interception. Optimised for seamless software integration, the framework facilitates rapid, hygienist-led risk stratification without increasing chairside burden, and delivers objective data streams to reinforce the dentist's definitive diagnostic workflow.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal and Peri-Implant Status Among Users of Different Inhaled Tobacco and Nicotine Products: A Systematic Review.","authors":"Maria Pia Di Palo, Federica Di Spirito, Francesco Giordano, Giuseppina De Benedetto, Roberto Lo Giudice, Federica Piedepalumbo, Gabriele Balsamo, Colomba Pessolano, Marina Garofano, Andrea Marino, Alessia Bramanti","doi":"10.3390/dj14090562","DOIUrl":"10.3390/dj14090562","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Periodontal and peri-implant diseases are influenced by several risk factors, including smoking. While cigarette smoking (CS) is a well-established risk factor, the impact of heated tobacco products (HTPs), electronic cigarettes (E-Cigs), and waterpipe (WS) remains less clear. This systematic review aimed to update the current evidence evaluating the clinical, radiographical, and inflammatory biomarkers of periodontal and peri-implant status among different untreated adult users of inhaled tobacco and nicotine products. <b>Methods</b>: A systematic search of PubMed/MEDLINE, Scopus, and Cochrane Library was performed for studies published between April 2022 and December 2025. Data were extracted and synthesized qualitatively according to the study protocol (PROSPERO: CRD420261284851). Studies were qualitatively assessed using the Risk-of-Bias in Nonrandomized-I. <b>Results</b>: Thirty-one studies including 3106 subjects were analyzed. CS consistently showed the worst periodontal and peri-implant status, with higher plaque indices, probing depth (PD), clinical attachment level (CAL), marginal bone loss (MBL) and more missing teeth. WS showed parameters comparable to CS and worse than non-smokers, with particularly high plaque accumulation and probing depth, as well as a pro-inflammatory biomarker profile. E-Cigs and HTPs generally exhibited intermediate status; E-Cigs showed increased PD and CAL compared with non-smokers, with elevated bleeding on probing despite a lower gingival index, whereas MBL was notably higher, showing the highest overall values among all smoking categories, exceeding CS. HTPs presented slightly less harmful outcomes than other smokers, but the evidence was limited. Biomarker analysis indicated elevated pro-inflammatory and tissue-destructive markers, particularly in CS and WS. <b>Conclusions</b>: Smoking negatively affects periodontal and peri-implant status, with CS exhibiting the worst impact. E-Cigs and HTPs appear less harmful but are not risk-free. Longitudinal studies are warranted to define long-term effects, particularly on peri-implant status, for which robust evidence was restricted to CS.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149037059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Proposed Parameter-Based Planning Framework for Inferior Alveolar Nerve Repositioning.","authors":"Fares Kablan, Asaf Zigron, Idan Redenski, Shadi Daoud, Amjad Shhadeh, Adeeb Zoabi, Samer Srouji","doi":"10.3390/dj14090561","DOIUrl":"10.3390/dj14090561","url":null,"abstract":"<p><p><b>Background</b>: Rehabilitation of the severely atrophic posterior mandible remains challenging when the residual bone height above the inferior alveolar nerve (IAN) is insufficient for conventional implant placement. In such cases, IAN lateralization or transposition may facilitate placement of longer implants and support fixed prosthetic rehabilitation. However, these procedures require careful case selection because of their technical complexity and the risk of neurosensory complications. <b>Objective</b>: This narrative review presents a literature-informed, expert-derived, parameter-based clinical decision framework for preoperative planning of IAN lateralization and transposition in the severely atrophic posterior mandible. <b>Methods</b>: A focused appraisal of the literature was performed to identify anatomical, prosthodontic, and surgical parameters relevant to IAN repositioning. Parameters were selected according to their recurrent relevance to surgical feasibility, implant positioning, procedural safety, and restorative planning, and were integrated with the authors' clinical experience to develop a structured decision-making framework. No formal risk-of-bias or certainty-of-evidence assessment was performed. Each parameter was assigned an evidence-source category and a threshold status to make its evidentiary basis explicit. <b>Results</b>: The proposed framework organizes treatment planning into three domains: bone-related, prosthodontic, and surgical considerations. Key parameters include residual bone height above the IAN canal, global mandibular height, ridge width, bone density, crown-to-implant ratio, intermaxillary space, ridge angulation, buccal cortical plate thickness, and the three-dimensional course of the IAN. Additional parameters include interforaminal dentition, implant trajectory, canal diameter, bifid canal anatomy, and ridge contour. Collectively, these variables are integrated into a stepwise decision pathway to help determine whether IAN repositioning is appropriate, whether lateralization or transposition is preferable, and when alternative approaches, such as short implants, ridge splitting, or vertical augmentation, may be more suitable. The framework links restorative feasibility and surgical risk to the choice of technique within a single sequential pathway, and reports the evidence basis of each decision point. <b>Conclusions</b>: The proposed framework should not be regarded as a validated clinical decision tool; prospective clinical validation and consensus-based refinement are required before routine clinical implementation. The framework is intended to structure and guide multidisciplinary assessment and discussion of cases; it should not independently determine treatment selection or replace individualized clinical judgment.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reliability of Root Canal Curvature Measurements and Canal Visibility Ratings over Repeated Calibration Rounds.","authors":"Mounir Ajjan Alhadid, Sabina Noreen Wuersching, Falk Schwendicke, Chantal Herbst, Sascha R Herbst","doi":"10.3390/dj14090553","DOIUrl":"10.3390/dj14090553","url":null,"abstract":"<p><p><b>Objectives:</b> To evaluate the inter- and intra-observer reliability of three methods for measuring root canal curvature and the reliability of canal visibility ratings on periapical radiographs over repeated calibration rounds. <b>Materials and Methods:</b> A total of 360 clinical periapical radiographs were divided into six batches and independently evaluated by three observers. Root canal curvature was measured using the Schneider, Weine, and a novel tangency-based method, while canal visibility was classified on a three-category scale. Inter- and intra-observer reliability were assessed using intraclass correlation coefficients (ICC). Agreement between observers was evaluated using Bland-Altman analysis, and differences between measurement methods were compared using repeated-measures analysis. The association between canal visibility and inter-observer measurement differences was assessed using Spearman rank correlation. <b>Results:</b> Inter-observer reliability for curvature measurements was predominantly good to excellent across all methods, with no consistent improvement over successive calibration rounds. Intra-observer reliability, assessed in one observer, was excellent (ICC 0.90-0.97). Measurement method significantly influenced angle values, with the Weine method producing larger angles than the Schneider and tangency-based methods, whereas the latter two showed comparable results. Agreement on canal visibility remained fair to moderate, and reduced canal visibility showed a weak but significant association with greater inter-observer differences in curvature measurements. <b>Conclusions:</b> All three methods demonstrated comparable inter-observer reliability, although the Weine method yielded systematically larger curvature angles. Agreement did not consistently improve over repeated calibration rounds. Reduced canal visibility may increase measurement variability and should be considered when interpreting root canal curvature on periapical radiographs.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Institutional Readiness for Orofacial Laserology Education in Thailand: A National Cross-Sectional Survey of Dental Schools.","authors":"Suwat Tanya, Chattarika Domdok, Patcharawan Srisilapanan","doi":"10.3390/dj14090559","DOIUrl":"10.3390/dj14090559","url":null,"abstract":"<p><strong>Background/objectives: </strong>Orofacial laserology is being increasingly incorporated into contemporary dental practice, yet its integration into dental education remains inconsistent. National evidence regarding institutional readiness for integrating orofacial laserology into dental curricula remains limited. This study examined the current status of orofacial laserology education in Thailand.</p><p><strong>Methods: </strong>A national cross-sectional survey was conducted between May and July 2026 among all accredited Thai dental schools. Academic leaders and full-time lecturers completed a structured online questionnaire addressing curriculum implementation, educational delivery, competency assessment, institutional readiness, perceived barriers, and future priorities. Descriptive and non-parametric statistical analyses were performed.</p><p><strong>Results: </strong>Fifteen of 18 dental schools (83.3%) participated, yielding 38 respondents. Undergraduate orofacial laserology education showed limited curricular integration, whereas postgraduate programs demonstrated broader implementation, particularly in hands-on, supervised clinical, and competency-based training. Institutional readiness did not differ between academic leaders and full-time lecturers (<i>p</i> > 0.05). Across historical phases of institutional establishment, perceived importance (<i>p</i> = 0.743) and interinstitutional collaboration (<i>p</i> = 0.800) were comparable, whereas institutional readiness differed significantly (<i>p</i> = 0.008), with regional pioneer dental schools reporting higher readiness than expansion-phase and contemporary dental schools. The principal barriers were insufficient qualified instructors, limited curriculum integration, and high equipment costs. Faculty development, funding and equipment support, and national competency frameworks were identified as the highest priorities.</p><p><strong>Conclusions: </strong>Orofacial laserology education in Thailand remains heterogeneous, with broader implementation in postgraduate than undergraduate programs. Strengthening faculty capacity, competency-based curricula, institutional support, and national collaboration may facilitate more consistent integration of orofacial laserology into dental curricula.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149037101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agreement Between Cone Beam Computed Tomography, Bone Sounding, Pulse-Echo Ultrasound, and Caliper Measurements for Gingival Thickness Assessment: A Method-Comparison Study.","authors":"Selma Dervisbegovic, Reinhard Gruber, Andrea Gamper, Stefan Nemec, Xiaohui Rausch-Fan, Gabriella Dvorak","doi":"10.3390/dj14090551","DOIUrl":"10.3390/dj14090551","url":null,"abstract":"<p><p><b>Background/Objectives</b>: Accurate assessment of gingival thickness (GT) is important for periodontal and implant treatment planning. <b>Methods</b>: In this method-comparison study based on prospectively collected clinical data from 2012, GT was assessed at standardized buccal sites using cone beam computed tomography (CBCT), bone sounding (BS), an earlier-generation pulse-echo ultrasound (US) device, and direct digital caliper (CA) measurement. Of 53 acquired CBCT scans, 12 (23%) were non-evaluable because the buccal soft tissues could not be reliably delineated, leaving 41 sites from 41 patients for analysis. A ±0.30 mm clinical comparison margin, based on the difference used in the original sample-size calculation, was applied to interpret agreement. <b>Results:</b> Mean comparator-minus-CBCT biases were -0.19 mm for BS (95% limits of agreement [LoA], -0.98 to 0.61 mm), -0.16 mm for US (95% LoA, -0.83 to 0.51 mm), and -0.71 mm for CA (95% LoA, -1.99 to 0.56 mm). Although BS and the tested US device showed smaller average differences from CBCT than CA, the LoA for all comparisons exceeded this margin. Phenotype classification was exploratory because only four sites were classified as thin by CBCT. <b>Conclusions</b>: BS and the tested pulse-echo US device showed smaller average differences from CBCT than CA; however, the limits of agreement for all comparisons exceeded this margin, so none of the comparator methods can be considered interchangeable with CBCT. The 23% non-evaluable CBCT rate and the device- and protocol-specific nature of the findings limit their generalizability to current-generation systems.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605818/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship Between Epstein-Barr Virus LMP-1 and BRAF-V600E Mutant Protein Expression in Ameloblastoma.","authors":"Kenko Okamoto, Michiko Nishimura, Yuji Miyazaki, Miyako Hoshino, Shinnichi Sakamoto, Miki Haruyama, Fumio Ide, Nobuharu Yamamoto, Kentaro Kikuchi","doi":"10.3390/dj14090556","DOIUrl":"10.3390/dj14090556","url":null,"abstract":"<p><p><b>Background</b>: Epstein-Barr virus (EBV) enters the human body via saliva. EBV-encoded latent membrane protein-1 (LMP-1) activates the mitogen-activated protein kinase (MAPK) pathway. MAPK signaling pathway activation due to the BRAF-V600E mutation is widely known as a major event in the pathogenesis of ameloblastoma. It is unclear whether EBV infects ameloblastoma and is involved in the pathogenesis of ameloblastoma. We investigated the relationship between LMP-1 and BRAF-V600E mutant protein expression in ameloblastoma by immunohistochemistry. <b>Methods</b>: We examined a total of 334 samples (ameloblastoma 117 samples, odontogenic keratocyst 127 samples, dentigerous cysts 45 samples, and dental follicle 45 samples). Ephrin type-A receptor 2 (EphA2), BRAF-V600E mutant protein, and γH2AX expression were confirmed by immunohistochemistry. We confirmed that the positive cases of BRAF-V600E mutant protein and γH2AX segregated based on LMP-1 expression: high grade (HG) and low grade (LG). <b>Results</b>: EphA2 expression was high in all lesions. The positive rate of BRAF-V600E mutant protein expression was significantly higher in ameloblastoma, and the positive rate of BRAF-V600E mutant protein in LMP-1 HG cases (71.9%) was significantly greater than in LG cases (39.6%) in ameloblastoma. The positive rate of γH2AX expression was significantly higher in ameloblastoma than in the other lesions. The positive rate of BRAF-V600E mutant protein in LMP-1 HG cases (71.9%) was significantly greater than in LG cases (39.6%) in ameloblastoma. <b>Conclusions</b>: BRAF-V600E mutant protein and γH2AX expression in ameloblastoma were significantly higher in LMP-1 HG cases than in LG cases.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"14 9","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13605404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149036868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}