Dentistry Journal最新文献

{"title":"A Review of White Spot Lesions: Development and Treatment with Resin Infiltration.","authors":"Alexandra Maria Prada, Georgiana Ioana Potra Cicalău, Gabriela Ciavoi","doi":"10.3390/dj12120375","DOIUrl":"10.3390/dj12120375","url":null,"abstract":"<p><p>White spot lesions (WSLs) are demineralized lesions of the enamel that form in the presence of bacterial plaque, affecting the aesthetics by modifying the refractive index of the enamel, giving the characteristic \"chalky\" aspect. They have various causes, including fixed orthodontic treatments, improper hygiene, fluorosis and genetic factors.</p><p><strong>Background/objectives: </strong>Considering the latest need for dental aesthetics and the popularization of fixed orthodontic treatments, the need to effectively treat WSLs has increased. The objective of this research is to review the development of WSLs and their treatment with resin infiltration.</p><p><strong>Methods: </strong>The PubMed, Web of Science, Scopus and Google Scholar databases were searched for relevant reviews and studies. Out of all, 56 were included in this research.</p><p><strong>Results: </strong>Prophylactic measures, such as fluorized toothpaste and varnishes, have limited results. Standard caries treatment is too invasive as it removes too much healthy enamel for obturation retentivity. The resin infiltration resin process does not require drilling or tooth structure loss, making it a painless and minimally invasive treatment. The resin used has a refractive index comparable to that of healthy enamel, consequently restoring aesthetics and ensuring the prevention of caries evolvement. The treatment involves five important steps: prophylaxis, acid demineralization, alcohol drying, resin infiltration and UV light curing. Depending on the clinical case, the demineralization and drying steps may need to be repeated.</p><p><strong>Conclusions: </strong>Infiltrations with resin are painless and well tolerated by patients. Out of all minimally invasive treatments, they have an immediate satisfactory outcome, with results stable for a minimum of 45 months.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bilateral Symmetry in the Aesthetic Area Achieved by Digital Smile Design on 3D Virtual Patient and Conventional Diagnostic Wax-Up-A Comparative Study.","authors":"Maria Hristozova, Mariya Dimitrova, Stefan Zlatev","doi":"10.3390/dj12120373","DOIUrl":"10.3390/dj12120373","url":null,"abstract":"<p><p><b>Background</b>: Digital diagnostic waxing is a contemporary alternative to the conventional wax-up method. This study aims to evaluate the impact of both techniques on the perceived frontal symmetry in aesthetic treatment planning. Dental symmetry significantly influences smile perception and, consequently, the acceptance of treatment outcomes, highlighting its clinical importance in restorative dentistry. <b>Materials and Methods</b>: A total of 100 teeth were measured, with 50 (<i>n</i> = 50) waxed up using traditional modeling techniques and 50 using a face-guided digital approach. The study involved ten patients requiring fixed restorations in the aesthetic zone. Both digital and conventional wax-ups were performed for each participant. Gypsum models with wax-ups were digitized and superimposed onto the digital diagnostic design using 3Shape Dental Designer Studio software, Version 2023 (3Shape, Copenhagen, Denmark). Screenshots of the frontal view were captured, and the width of each morphologically altered tooth was measured using ImageJ software Version 1.54 (National Institutes of Health, Bethesda, MD, USA). <b>Results</b>: The results indicated no statistically significant difference in symmetry between the right and left sides achieved by the two diagnostic approaches (t-value = -1.89, <i>p</i>-value = 0.07). The perceived symmetry of morphologically modified frontal teeth, as achieved by digital and conventional waxing, was found to be comparable. <b>Conclusions</b>: Digital diagnostic planning is validated as a reliable alternative to the conventional wax-up method, offering comparable accuracy in achieving dental symmetry while potentially enhancing efficiency and precision in the aesthetic treatment planning process. This result underscores the potential of digital technologies to streamline clinical workflows and improve patient outcomes. Clinically, achieving symmetry in the aesthetic zone is crucial for patient satisfaction and acceptance of restorative procedures, emphasizing the need for continued integration of digital tools in dental practice.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating Craniofacial Morphology Ratios as Predictors of Obstructive Sleep Apnea Severity in Non-Obese Adult Males.","authors":"Masasuke Shimatsu, Shigeto Kawashima, Mitsuyoshi Suzuki","doi":"10.3390/dj12120374","DOIUrl":"10.3390/dj12120374","url":null,"abstract":"<p><p><b>Background</b>: This study aimed to examine the connection between craniofacial morphology, particularly the horizontal and vertical dimensions of the mandible, and the severity of obstructive sleep apnea (OSA) in non-obese adult males by utilizing a cephalometric analysis and introducing a new skeletal ratio index. <b>Methods</b>: A cohort of 44 non-obese adult males with OSA, diagnosed via the apnea-hypopnea index (AHI) from polysomnographic recordings, was evaluated using a lateral cephalometric analysis. OSA severity was classified as mild (5 ≤ AHI < 15) in 19 patients, moderate (15 ≤ AHI < 30) in 15 patients, and severe (AHI ≥ 30) in 10 patients. The S-Go distance divided by the N-Me distance (S-Go/N-Me) was used as a vertical ratio of craniofacial morphology, the Go-Me distance divided by the S-N distance (Go-Me/S-N) was used as a horizontal ratio, and the results were compared between groups. Correlations between each ratio and craniofacial morphology based on the five factors from the Ricketts analysis were examined for each group. <b>Results</b>: A significant difference was found in the horizontal ratio Go-Me/S-N between the mild and moderate groups (<i>p</i> < 0.05) and the mild and severe groups (<i>p</i> < 0.05). However, no significant differences in Ricketts analysis factors were observed across OSA severity groups. Correlations between the Go-Me/S-N and Ricketts factors were identified in the mild and moderate groups but not in the severe group. The horizontal skeletal dimension Go-Me/S-N was strongly associated with OSA severity. <b>Conclusions</b>: The horizontal mandibular ratio Go-Me/S-N, independent of body shape, may offer a valuable morphological marker for differentiating OSA severity in non-obese males.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 12","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674528/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142892870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Inflammatory Scores in Severity Prediction for Elderly Patients with Odontogenic Infections.","authors":"Gianina Tapalaga, Luminita Maria Nica, Laura-Elena Cirligeriu, Bogdan Andrei Bumbu, Marius Pricop","doi":"10.3390/dj12110371","DOIUrl":"10.3390/dj12110371","url":null,"abstract":"<p><p><b>Background and Objectives:</b> Odontogenic infections (OIs) can lead to severe complications, especially in elderly patients due to age-related physiological changes and comorbidities. This study aims to evaluate the predictive accuracy of inflammatory scores-APACHE II, CURB-65, SOFA, and NEWS2-in determining the severity of odontogenic infections among elderly patients (>70 years) compared to younger patients (<70 years). <b>Materials and Methods:</b> A retrospective cohort study was conducted on patients diagnosed with an OI at the Maxillofacial Surgery Department between January 2018 and January 2024. Patients were divided into two groups: elderly patients (>70 years, n = 49) and younger patients (<70 years, n = 64). The Symptom Severity score (SS) of odontogenic infections was calculated for all patients. Inflammatory scores-APACHE II, CURB-65, SOFA, and NEWS2-were assessed at admission and correlated with infection severity. Additional subgroup analyses were performed based on comorbidities and infection sites. <b>Results:</b> Elderly patients exhibited significantly higher SS scores (mean 12.47 ± 2.93) compared to younger patients (mean 7.82 ± 2.17, <i>p</i> < 0.001). APACHE II, CURB-65, SOFA and NEWS2 scores were significantly elevated in the elderly group (all <i>p</i> < 0.001). The SOFA score demonstrated the highest predictive accuracy for severe OIs in elderly patients, with an area under the curve (AUC) of 0.89 (95% CI: 0.82-0.95). Subgroup analyses revealed that comorbidities such as diabetes mellitus and cardiovascular disease significantly influenced infection severity (<i>p</i> < 0.05). <b>Conclusions:</b> Inflammatory scores, particularly SOFA, are effective in predicting the severity of odontogenic infections in elderly patients. The integration of these scores into clinical practice may enhance early identification of high-risk patients and improve management strategies.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term Stability in Temporomandibular Joint Replacement: A Review of Related Variables.","authors":"Erick Vargas, Victor Ravelo, Majeed Rana, Alejandro Unibazo, Sergio Olate","doi":"10.3390/dj12110372","DOIUrl":"10.3390/dj12110372","url":null,"abstract":"<p><p><b>Background:</b> The temporomandibular joint (TMJ) is a key component of the stomatognathic system, playing a major role in maintaining mandibular stability and function. Temporomandibular disorders (TMDs) are a prevalent disease in the world, with surgical treatment being reserved for complex cases or end-stage TMJ disease. A narrative review was conducted to describe the quantitative and qualitative factors that affect TMJ prosthesis stability. <b>Methods:</b> Studies with a sample size equal to or greater than 10 subjects who underwent surgical procedures for joint replacement using stock or customized ATM prostheses were included. This narrative review examined some variables that may influence in terms of the longevity of the TMJ prosthesis, highlighting issues to be considered in future research. <b>Results:</b> The current development of TMJ prostheses is benefiting from technological advances, offering a suitable adaptation to the patient's anatomy and superior results in functionality and patient satisfaction. However, the biomechanical complexity of the TMJ shows unique challenges compared to other joints in the body, where anatomical, biomechanical, and functional requirements are high. The stability of the TMJ prosthesis is affected by multiple variables, including the selection of biocompatible materials that resist corrosion and wear, the design of the prosthesis, the diagnosis and indication for its use, and the surgeon's experience. The success of TMJ replacement can be measured by improving the patient's quality of life, reducing pain, restoring mandibular functionality, and recovering suitable facial morphology for the patient's conditions. <b>Conclusion:</b> There is a need for training of maxillofacial surgeons in TMJ surgery and replacement, as well as a greater focus on the research and development of systems to simplify surgical design and procedures and to optimize the results of TMJ replacement.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mandibular Advancement Devices in OSA Patients: Impact on Occlusal Dynamics and Tooth Alignment Modifications-A Pilot Prospective and Retrospective Study.","authors":"Aylin Uyaner, Helen Schneider, Aditya Parikh, Kathrin Paeske-Hinz, Anna Konermann","doi":"10.3390/dj12110370","DOIUrl":"10.3390/dj12110370","url":null,"abstract":"<p><p><b>Background</b>: The widespread prevalence of obstructive sleep apnea (OSA) underscores the necessity for effective therapies. Mandibular advancement devices (MADs) have emerged as valid treatment for mild to moderate cases, despite the associated dental side effects. <b>Methods</b>: This study evaluates the nature, onset, and long-term manifestation of these side effects. In the prospective group (<i>n</i> = 12), dental impressions were taken pre-MAD-insertion and at intervals of three, six, nine, and twelve months post-insertion to monitor occlusal alterations. In the retrospective group, participants (<i>n</i> = 8) wearing MADs for 7 years at average underwent lateral cephalogram assessments to compare with pre-treatment X-rays. All participants completed a specific questionnaire. Statistical analysis was performed via <i>t</i>-test and with <i>p</i> < 0.05 as the significance level. <b>Results</b>: The majority of participants in both groups consistently used MADs and reported significant sleep quality improvements, rating common symptoms like jaw tension as negligible. In both the prospective group and the retrospective group, significant reductions in overjet were observed at multiple time points, with the prospective group showing reductions at six months (<i>p</i> = 0.001), nine months (<i>p</i> > 0.001), and twelve months (<i>p</i> = 0.019), while the retrospective group indicated a notable decrease between baseline and follow-up assessments after a mean of seven years of device wear (<i>p</i> = 0.004). A slight overbite increase of 0.2 mm was prospectively observed after one year, whereas a trend towards a minimal decrease over the long term was observed in the retrospective sample (<i>p</i> = 0.003). Noteworthy changes in angle class or lower incisor inclination were absent. Cephalograms revealed significant IOK-NL angle alterations with a mean of 98.2° before and 95.2° upon long-term treatment (<i>p</i> = 0.020). <b>Conclusions</b>: These findings suggest that MADs are effective in treating OSA with minor adverse effects. This study advocates for moderate mandibular protrusion to balance therapeutic efficacy with dental health considerations, crucial for optimizing treatment outcomes. Nonetheless, the limited sample size warrants caution when generalizing these results to the broader population.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592911/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital Condylar Parameter Assessment Using Cadiax^® 2 and Modjaw^®.","authors":"Smaranda Buduru, Sara Hafidi, Oana Almășan, Manuela Manziuc, Manuela Tăut, Rareș Buduru, Vlad-Ionuț Nechita, Andreea Kui, Andreea Chisnoiu, Cecilia Bacali","doi":"10.3390/dj12110369","DOIUrl":"10.3390/dj12110369","url":null,"abstract":"<p><p><b>Background</b>: The main aim of this research was to assess the reliability of two systems designed specifically for condylar movement recording using condylar slope and Bennett angle information. The objectives were to evaluate the validity of two subsequent null hypotheses: (1) there is no significant difference between the measurements of condylar slope and Bennett angle taken at T0 (initial) and T1 (after one week) using the same equipment; (2) there is no notable difference in the values of the condylar slope and Bennett angle measurements obtained using Modjaw and Cadiax 2. <b>Methods</b>: An observational, descriptive, and prospective study was conducted with a selected group of 25 individuals (13 females and 12 males) aged between 22 and 27. <b>Results</b>: The results of Cadiax 2 and Modjaw showed excellent measurement repeatability for both parameters, with intraclass correlation coefficients (ICC) above 0.90, indicating excellent reliability between T0 and T1, both at 3 mm and 5 mm of displacement. Modjaw had an overall average value relatively higher than Cadiax 2, even though Modjaw's condylar slope at 5mm had a significantly lower average value (37.4 ± 6.31) with an interval of 24.5-48.1, which was lower than Cadiax 2 (48.4 ± 10.6) with an interval of 30.5-68.5. Regarding the primary aim, it can be stated that both Modjaw and Cadiax 2 demonstrated excellent repeatability on their own, demonstrating robust reliability since there was no discernible difference between the T0 and T1 measurements. On the contrary, analyses of the two devices' measured values for the secondary aim showed a considerable difference. <b>Conclusions</b>: Even though each device is reliable on its own, the absolute values that are obtained are different. Technological differences between the systems may account for these variations.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Comfort Level with the Non-Sliding Lingual Orthodontic Technique (BRIUS™) Versus Labial Full Fixed Appliances: A Pilot Randomized Controlled Clinical Trial.","authors":"Mohammed Hussain Alzainal, Stephen Warunek, David A Covell, William Tanberg, Aron Aliaga-Del Castillo, Lucia Cevidanes, Thikriat Al-Jewair","doi":"10.3390/dj12110368","DOIUrl":"10.3390/dj12110368","url":null,"abstract":"<p><p><b>Introduction:</b> This pilot two-arm, parallel group, randomized clinical trial aimed to compare the efficacy of tooth movement and patient comfort during orthodontic leveling and alignment with the BRIUS™ lingual system (BR) versus labial full fixed appliances (LFFAs). <b>Methods:</b> Patients in the permanent dentition with mild to moderate crowding were recruited at the University at Buffalo and randomly assigned into the BR group (seven patients) or LFFAs group (six patients). The patients' dental arches were scanned before bonding (T1) and after 18 weeks (T2). Digital dental model superimpositions were performed to three-dimensionally evaluate tooth movement. Changes between T1 and T2 were measured in the x, y, and z planes. Little's Irregularity Index (LII) was also assessed at T1 and T2. An electronic questionnaire was completed daily for 7 days after T1 to evaluate the patients' comfort levels. <b>Results:</b> After 18 weeks, similar displacements were observed for all teeth in both groups except for the lower left second premolar (LL5) in the x-axis which showed greater displacement in the BR group (<i>p</i> = 0.016). Groups showed similar changes in LII. Discomfort during the first week after bonding was greater on the tongue in the BR group and on the lips and cheeks in the LFFAs group. Tongue discomfort caused by the BR lasted for around 3 days. <b>Conclusion:</b> The BR and LFFAs showed similar teeth displacements and therefore were equally effective at leveling and aligning teeth. LFFAs caused cheek- and lip-borne discomfort while the BR caused tongue-borne discomfort during the first week after bonding. Larger studies with longer follow-ups are needed to obtain more definitive results.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11593093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142727029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An In Vivo Comparison of Trueness and Precision of Two Novel Methods for Improving Edentulous Full Arch Implant Scanning Accuracy: A Pilot Study.","authors":"Adam Brian Nulty","doi":"10.3390/dj12110367","DOIUrl":"10.3390/dj12110367","url":null,"abstract":"<p><p><b>Background:</b> This retrospective in vivo study evaluated the trueness and precision of two digital intraoral scanners-Dentsply Sirona Primescan and Medit i900-, both with and without two variants of the novel Scan Ladder aids, and compared their performance to a new intraoral photogrammetry scanner (Shining 3D Elite). <b>Methods:</b> Data from ten edentulous patients, previously collected during routine clinical treatment, were analyzed using a master STL generated from traditional impression casts as the reference. A custom positional change calculator and comprehensive statistical analysis were used to assess scanner accuracy. <b>Results:</b> The findings demonstrated that the use of the Scan Ladder significantly enhanced the overall accuracy of both intraoral scanners, showing no statistically significant differences when compared to the intraoral photogrammetry system. <b>Conclusions:</b> These results indicate that the Scan Ladder improves the performance of conventional intraoral scanners and suggests that the Shining 3D Elite intraoral photogrammetry scanner is a reliable alternative to extraoral photogrammetry for edentulous cases. Further research, with a larger and more diverse cohort, is warranted to validate and expand upon these findings.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592884/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular Phosphate Modulation and Polyphosphate Accumulation by <i>Corynebacterium matruchotii</i> and <i>Streptococcus mutans</i>.","authors":"Debarati Ghose, Robert S Jones","doi":"10.3390/dj12110366","DOIUrl":"10.3390/dj12110366","url":null,"abstract":"<p><p>(1) Background: An alternative and understudied microbial mechanism that may influence demineralization is the microbially mediated ion exchange of Ca²⁺ and orthophosphate (P_i), which alters the saturation state of the mineral species within the surface enamel. There is a need to examine the ability of members of the oral microbiome to modulate Ca²⁺ and P_i, which control mineral solubility, in order to effectively evaluate mineralization therapies to improve oral health. (2) Methods: P_i uptake was measured using an ascorbic acid assay during a BHI liquid culture growth of <i>Corynebacterium matruchotii</i> and <i>Streptococcus mutans</i> for up to 20 h. The initial and endpoint medium Ca²⁺ levels were measured using ICP-OES. Bacterial cells were examined at different growth stages using DAPI/polyP binding emission at 525 nm to detect the presence of internalized macromolecules of polyphosphates (polyP) that could drive P_i uptake. (3) Results: <i>C. matruchotii</i> (<i>p</i> = 0.0061) substantially accumulated P_i (3.84 mmol/L), with a concomitant formation of polyP. In contrast, <i>S. mutans did</i> not take up P_i or accumulate polyP. No significant Ca²⁺ drawdown in the media was observed in either strain. (4) Conclusions: This study suggests that when examining the future efficacy of prevention technologies to improve, in vitro assays may consider including specific oral bacteria capable of substantial P_i uptake.</p>","PeriodicalId":11269,"journal":{"name":"Dentistry Journal","volume":"12 11","pages":""},"PeriodicalIF":2.5,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11592680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142726975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}