Cell host & microbe最新文献_第2页

Boosting endosymbiosis in plants for future self-sustained crop production

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-12 DOI: 10.1016/j.chom.2025.02.011

Ariane Kemen, Eric Kemen

引用次数: 0

Plant growth promotion through repression of defenses by leaf microbiota

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-12 DOI: 10.1016/j.chom.2025.02.010

Benjamin Gourion

引用次数: 0

Rewild your gut: Fighting modern diseases with ancient microbes

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-12 DOI: 10.1016/j.chom.2025.02.012

Gwenn Pinel, André Marette

引用次数: 0

Plant cell-cycle regulators control the nuclear environment for viral pathogenesis 植物细胞周期调节器控制病毒致病的核环境

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-04 DOI: 10.1016/j.chom.2025.02.006

Xu Zhang, Ge Wang, Peng Zhang, Chunyan Chen, Jiucheng Zhang, Yumei Bian, Minmin Liu, Chenxu Niu, Fengze Sun, Yahui Wang, Genzhong Liu, Zhimin Wang, Fangfang Ma, Zhilong Bao

{"title":"Plant cell-cycle regulators control the nuclear environment for viral pathogenesis","authors":"Xu Zhang, Ge Wang, Peng Zhang, Chunyan Chen, Jiucheng Zhang, Yumei Bian, Minmin Liu, Chenxu Niu, Fengze Sun, Yahui Wang, Genzhong Liu, Zhimin Wang, Fangfang Ma, Zhilong Bao","doi":"10.1016/j.chom.2025.02.006","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.006","url":null,"abstract":"The proper regulation of cell-cycle regulators is curial for both viral replication and host-plant adaptive growth during the viral pathogenesis. Mechanisms on reorchestrating RETINOBLASTOMA-RELATED 1 (RBR1), repressor of E2F transcription factor, and downstream genes in host-virus interactions are unclear. Here, we discover that anaphase-promoting complex/cyclosome (APC/C) E3 ligase activator cell division cycle 20 (CDC20) in tomato binds RBR1 or mediates cyclin D1 depletion to preserve RBR1-E2F complexes, while geminivirus or crinivirus repurposes APC/CCDC20 activities to liberate E2Fs in two ways: activating APC/CCDC20 to deplete RBR1 or blocking APC/CCDC20 to stimulate cyclin-D1-mediated RBR1 depletion. The liberated E2Fs activate DNA polymerase or heat shock protein 70 gene transcription to favor virus propagation. The improper disruption of RBR1-E2F complexes via hijacking APC/CCDC20 causes the host growth repression. We uncover a scenario in which the virus co-opts host APC/CCDC20 to reprogram RBR1-E2F complex to favor its propagation while dampening host vitality.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"9 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cross-kingdom-mediated detection of intestinal protozoa through NLRP6 通过 NLRP6 跨领域检测肠道原生动物

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-04 DOI: 10.1016/j.chom.2025.02.008

Nathaniel J. Winsor, Giuliano Bayer, Ojas Singh, Jeremy K. Chan, Lu Yi Li, Brandon Y. Lieng, Elisabeth Foerster, Ana Popovic, Boyan K. Tsankov, Heather Maughan, Paul Lemire, Elaine Tam, Catherine Streutker, Lina Chen, Stacey L. Heaver, Ruth E. Ley, John Parkinson, J. Rafael Montenegro-Burke, George M.H. Birchenough, Dana J. Philpott, Stephen E. Girardin

{"title":"Cross-kingdom-mediated detection of intestinal protozoa through NLRP6","authors":"Nathaniel J. Winsor, Giuliano Bayer, Ojas Singh, Jeremy K. Chan, Lu Yi Li, Brandon Y. Lieng, Elisabeth Foerster, Ana Popovic, Boyan K. Tsankov, Heather Maughan, Paul Lemire, Elaine Tam, Catherine Streutker, Lina Chen, Stacey L. Heaver, Ruth E. Ley, John Parkinson, J. Rafael Montenegro-Burke, George M.H. Birchenough, Dana J. Philpott, Stephen E. Girardin","doi":"10.1016/j.chom.2025.02.008","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.008","url":null,"abstract":"Intestinal protists are detected by the host innate immune system through mechanisms that remain poorly understood. Here, we demonstrate that Tritrichomonas protozoa induce thickening of the colonic mucus in an NLRP6-, ASC-, and caspase-11-dependent manner, consistent with the activation of sentinel goblet cells. Mucus growth is recapitulated with cecal extracts from Tritrichomonas-infected mice but not purified protozoa, suggesting that NLRP6 may detect infection-induced microbial dysbiosis. In agreement, Tritrichomonas infection causes a shift in the microbiota with the expansion of Bacteroides and Prevotella, and untargeted metabolomics reveals a dramatic increase in several classes of metabolites, including sphingolipids. Finally, using a combination of gnotobiotic mice and ex vivo mucus analysis, we demonstrate that wild-type, but not sphingolipid-deficient, B. thetaiotaomicron is sufficient to induce NLRP6-dependent sentinel goblet cell function, with the greatest effect observed in female mice. Thus, we propose that NLRP6 is a sensor of intestinal protozoa infection through monitoring microbial sphingolipids.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"29 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-03 DOI: 10.1016/j.chom.2025.02.005

Gaopeng Hou, Juhee Son, Maria Florencia Gomez Castro, Takahiro Kawagishi, Xingxing Ren, Alexa N. Roth, Avan Antia, Qiru Zeng, Anna L. DeVeaux, Ningguo Feng, Hinissan P. Kohio, Megan T. Baldridge, Terence S. Dermody, Shu Zhu, Siyuan Ding

{"title":"Innate immune sensing of rotavirus by intestinal epithelial cells leads to diarrhea","authors":"Gaopeng Hou, Juhee Son, Maria Florencia Gomez Castro, Takahiro Kawagishi, Xingxing Ren, Alexa N. Roth, Avan Antia, Qiru Zeng, Anna L. DeVeaux, Ningguo Feng, Hinissan P. Kohio, Megan T. Baldridge, Terence S. Dermody, Shu Zhu, Siyuan Ding","doi":"10.1016/j.chom.2025.02.005","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.005","url":null,"abstract":"Diarrhea is the predominant symptom of acute gastroenteritis resulting from enteric infections and a leading cause of death in infants and young children. However, the role of the host response in diarrhea pathogenesis is unclear. Using rotavirus and neonatal mice as a model, we found that oral inoculation of UV-inactivated replication-defective rotavirus consistently induced watery diarrhea by robust activation of cytosolic double-stranded RNA sensing pathways and type III interferon (IFN-λ) secretion. Diarrhea was significantly diminished in mice lacking the IFN-λ receptor. Mechanistically, IFN-λ signaling downregulates the expression of Dra, a chloride and bicarbonate exchanger, which contributes to reduced water absorption. We confirmed these findings in mice inoculated with reovirus, as well as in donor-derived human intestinal organoids and human biopsy samples. Our data highlight a mechanism of rapid diarrhea induction by host innate immune sensing in the gastrointestinal tract and suggest that diarrhea induction is an active host defense strategy to eliminate the pathogen.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"5 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A designed synthetic microbiota provides insight to community function in Clostridioides difficile resistance

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-03-03 DOI: 10.1016/j.chom.2025.02.007

Shuchang Tian, Min Soo Kim, Jingcheng Zhao, Kerim Heber, Fuhua Hao, David Koslicki, Sangshan Tian, Vishal Singh, Andrew D. Patterson, Jordan E. Bisanz

{"title":"A designed synthetic microbiota provides insight to community function in Clostridioides difficile resistance","authors":"Shuchang Tian, Min Soo Kim, Jingcheng Zhao, Kerim Heber, Fuhua Hao, David Koslicki, Sangshan Tian, Vishal Singh, Andrew D. Patterson, Jordan E. Bisanz","doi":"10.1016/j.chom.2025.02.007","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.007","url":null,"abstract":"Clostridioides difficile, a major cause of antibiotic-associated diarrhea, is suppressed by the gut microbiome, but the precise mechanisms are not fully described. Through a meta-analysis of 12 human studies, we designed a synthetic fecal microbiota transplant (sFMT1) by reconstructing microbial networks negatively associated with C. difficile colonization. This lab-built 37-strain consortium formed a functional community suppressing C. difficile in vitro and in animal models. Using sFMT1 as a tractable model system, we find that bile acid 7α-dehydroxylation is not a determinant of sFMT1 efficacy while one strain performing Stickland fermentation—a pathway of competitive nutrient utilization—is both necessary and sufficient for the suppression of C. difficile, replicating the efficacy of a human fecal transplant in a gnotobiotic mouse model. Our data illustrate the significance of nutrient competition in suppression of C. difficile and a generalizable approach to interrogating complex community function through robust methods to leverage publicly available sequencing data.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"8 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neutrophil recruitment during intestinal inflammation primes Salmonella elimination by commensal E. coli in a context-dependent manner

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-02-28 DOI: 10.1016/j.chom.2025.02.004

Yassine Cherrak, Andrew Abi Younes, Eugenio Perez-Molphe-Montoya, Luca Maurer, Koray Yilmaz, Ursina Enz, Christophe Zeder, Patrick Kiefer, Philipp Christen, Ersin Gül, Julia A. Vorholt, Christian von Mering, Wolf-Dietrich Hardt

{"title":"Neutrophil recruitment during intestinal inflammation primes Salmonella elimination by commensal E. coli in a context-dependent manner","authors":"Yassine Cherrak, Andrew Abi Younes, Eugenio Perez-Molphe-Montoya, Luca Maurer, Koray Yilmaz, Ursina Enz, Christophe Zeder, Patrick Kiefer, Philipp Christen, Ersin Gül, Julia A. Vorholt, Christian von Mering, Wolf-Dietrich Hardt","doi":"10.1016/j.chom.2025.02.004","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.004","url":null,"abstract":"Foodborne bacterial diarrhea involves complex pathogen-microbiota-host interactions. Pathogen-displacing probiotics are increasingly popular, but heterogeneous patient outcomes highlighted the need to understand individualized host-probiotic activity. Using the mouse gut commensal Escherichia coli 8178 and the human probiotic E. coli Nissle 1917, we found that the degree of protection against the enteric pathogen Salmonella enterica serovar Typhimurium (S. Tm) varies across mice with distinct gut microbiotas. Pathogen clearance is linked to enteropathy severity and subsequent recruitment of intraluminal neutrophils, which differs in a microbiota-dependent manner. By combining mouse knockout and antibody-mediated depletion models with bacterial genetics, we show that neutrophils and host-derived reactive oxygen species directly influence E. coli-mediated S. Tm displacement by potentiating siderophore-bound toxin killing. Our work demonstrates how host immune factors shape pathogen-displacing probiotic efficiency while also revealing an unconventional antagonistic interaction where a gut commensal and the host synergize to displace an enteric pathogen.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"85 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143518115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria sense bacterial lactate and drive release of neutrophil extracellular traps

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-02-27 DOI: 10.1016/j.chom.2025.02.003

Ashley D. Wise, Eden G. TenBarge, Jessica d.C. Mendonça, Ellie C. Mennen, Sarah R. McDaniel, Callista P. Reber, Bailey E. Holder, Madison L. Bunch, Eva Belevska, Madalyn G. Marshall, Nicole M. Vaccaro, Christian R. Blakely, Dinesh H. Wellawa, Jennifer Ferris, Jessica R. Sheldon, Jeffry D. Bieber, Jeremiah G. Johnson, Lindsey R. Burcham, Andrew J. Monteith

{"title":"Mitochondria sense bacterial lactate and drive release of neutrophil extracellular traps","authors":"Ashley D. Wise, Eden G. TenBarge, Jessica d.C. Mendonça, Ellie C. Mennen, Sarah R. McDaniel, Callista P. Reber, Bailey E. Holder, Madison L. Bunch, Eva Belevska, Madalyn G. Marshall, Nicole M. Vaccaro, Christian R. Blakely, Dinesh H. Wellawa, Jennifer Ferris, Jessica R. Sheldon, Jeffry D. Bieber, Jeremiah G. Johnson, Lindsey R. Burcham, Andrew J. Monteith","doi":"10.1016/j.chom.2025.02.003","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.003","url":null,"abstract":"Neutrophils induce oxidative stress, creating a harsh phagosomal environment. However, Staphylococcus aureus can survive these conditions, requiring neutrophils to deploy mechanisms that sense bacterial persistence. We find that staphylococcal lactate is a metabolic danger signal that triggers neutrophil extracellular trap release (NETosis). Neutrophils coordinate mitochondria in proximity to S. aureus-containing phagosomes, allowing transfer of staphylococcal lactate to mitochondria where it is rapidly converted into pyruvate and causes mitochondrial reactive oxygen species, a precursor to NETosis. Similar results were observed in response to phylogenetically distinct bacteria, implicating lactate accumulation as a broad signal triggering NETosis. Furthermore, patients with systemic lupus erythematosus (SLE) are more susceptible to bacterial infections. We find that SLE neutrophils cannot sense bacterial lactate impairing their capacity to undergo NETosis upon S. aureus infection but initiate aberrant NETosis triggered by apoptotic debris. Thus, neutrophils adapt mitochondria as sensory organelles that detect bacterial metabolic activity and dictate downstream antibacterial processes.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"41 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Individual leaf microbiota tunes a genetic regulatory network to promote leaf growth

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-02-27 DOI: 10.1016/j.chom.2025.02.002

Valéria Custódio, Isai Salas-González, David Gopaulchan, Paulina Flis, Regla Amorós-Hernández, Yi-Qun Gao, Xianqing Jia, Ângela Moreno, Esther Carrera, Caroline Marcon, Frank Hochholdinger, M. Margarida Oliveira, David E. Salt, Gabriel Castrillo

{"title":"Individual leaf microbiota tunes a genetic regulatory network to promote leaf growth","authors":"Valéria Custódio, Isai Salas-González, David Gopaulchan, Paulina Flis, Regla Amorós-Hernández, Yi-Qun Gao, Xianqing Jia, Ângela Moreno, Esther Carrera, Caroline Marcon, Frank Hochholdinger, M. Margarida Oliveira, David E. Salt, Gabriel Castrillo","doi":"10.1016/j.chom.2025.02.002","DOIUrl":"https://doi.org/10.1016/j.chom.2025.02.002","url":null,"abstract":"In natural ecosystems, microbes have the ability to stably colonize plant leaves, overcoming the fluctuating environmental conditions that the leaves represent. How the phyllosphere microbiota influences the growth of individual leaves remains poorly understood. Here, we investigate the growth of Zea mays (maize/corn) leaves in plants grown in three soils with differing amounts of nutrients and water and identify a leaf-growth-promoting effect driven by the leaf microbiota, which we also validate in field studies. We built and used a bacterial strain collection for recolonization experiments to study the microbiota-mediated mechanisms involved in leaf growth promotion. We demonstrate that prevalent bacteria inhabiting young leaves promote individual leaf growth. Using transcriptomic analyses, we reveal a defense-related genetic network that integrates the beneficial effect of the phyllosphere microbiota into the leaf development program. We demonstrate that the individual leaf microbiota differentially represses this genetic network to modulate the growth-defense trade-off at single-leaf resolution.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"6 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143506853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0