Cell host & microbe最新文献

Systematic, high-throughput characterization of bacteriophage gene essentiality on diverse hosts 系统的，高通量的表征噬菌体基因的必要性在不同的宿主

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-22 DOI: 10.1016/j.chom.2025.06.018

Jackie Chen, Erick D. Nilsen, Chutikarn Chitboonthavisuk, James E. Corban, Matthew Yang, Charlie Y. Mo, Srivatsan Raman

{"title":"Systematic, high-throughput characterization of bacteriophage gene essentiality on diverse hosts","authors":"Jackie Chen, Erick D. Nilsen, Chutikarn Chitboonthavisuk, James E. Corban, Matthew Yang, Charlie Y. Mo, Srivatsan Raman","doi":"10.1016/j.chom.2025.06.018","DOIUrl":"https://doi.org/10.1016/j.chom.2025.06.018","url":null,"abstract":"Understanding core and conditional gene essentiality is crucial for decoding genotype-phenotype relationships in organisms. We present phage high-throughput approach for gene essentiality mapping and profiling (PhageMaP), a high-throughput method to create genome-scale loss-of-function libraries for systematically assessing gene essentiality in bacteriophages. Using PhageMaP across diverse hosts, we generate gene essentiality maps for all or most genes in the model phages T7 and T4, as well as the non-model phage Bas63. These maps provide fundamental insights into genome organization, gene function, and host-specific conditional essentiality. By applying PhageMaP to a collection of anti-phage defense systems, we uncover phage genes that either inhibit or activate six defenses and offer mechanistic hypotheses. Furthermore, we engineer synthetic phages with enhanced infectivity by modular transfer of a PhageMaP-discovered defense inhibitor from Bas63 to T7. PhageMaP advances bacteriophage functional genomics and accelerates rational phage design for therapy.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"14 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144678135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metatranscriptomics catches gut microbes in the act 超转录组学捕捉到了肠道微生物的活动

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.004

Kailin Liu, Yuhao Wang

引用次数: 0

How hosts accurately command bacteria for gut health 宿主如何准确地控制细菌以保持肠道健康

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.008

Jianglin Zhang, Xianda Ma, Zheng Kuang

引用次数: 0

Theory of host-microbe symbioses: Challenges and opportunities 宿主-微生物共生理论：挑战与机遇

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.05.001

Pamela Ferretti, Maria M. Martignoni, Lisa C. McManus, Taom Sakal, Armun Liaghat, Bethany Stevens, Kyle J.-M. Dahlin, Lucas S. Souza, Zoe G. Cardon, Cynthia B. Silveira, Seth R. Bordenstein, Joan Roughgarden

引用次数: 0

Waste as an antibacterial weapon 废物作为抗菌武器

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.011

M. Maria Elgrail, Michael S. Glickman

引用次数: 0

Bacteriophage genomes encode both broad and specific counter-defense repertoires to overcome bacterial defense systems 噬菌体基因组编码广泛和特定的反防御谱以克服细菌防御系统

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.010

Ana Rita Costa, Daan F. van den Berg, Jelger Q. Esser, Halewijn van den Bossche, Nadiia Pozhydaieva, Konstantinos Kalogeropoulos, Stan J.J. Brouns

{"title":"Bacteriophage genomes encode both broad and specific counter-defense repertoires to overcome bacterial defense systems","authors":"Ana Rita Costa, Daan F. van den Berg, Jelger Q. Esser, Halewijn van den Bossche, Nadiia Pozhydaieva, Konstantinos Kalogeropoulos, Stan J.J. Brouns","doi":"10.1016/j.chom.2025.06.010","DOIUrl":"https://doi.org/10.1016/j.chom.2025.06.010","url":null,"abstract":"The evolutionary arms race between bacteria and bacteriophages drives rapid evolution of bacterial defense mechanisms with scattered distribution across genomes. We hypothesized that this variability in bacterial defense systems leads to equally variable counter-defense repertoires in phage genomes. Examining the variable regions in Pseudomonas model phages of the Pbunavirus genus revealed five anti-defense genes, including one inhibiting Druantia type III named DadIII-1, another targeting Thoeris type III named TadIII-1, one inhibiting Zorya type I named ZadI-1, and two related broad defense inhibitors named Bdi1 and Bdi2 targeting four defenses. A typical Pbunavirus encodes up to five known anti-defense genes, some inhibiting four unrelated defense systems with distinct nucleic-acid-targeting mechanisms. Structural homologs of broad-acting Bdi1 and Bdi2 are encoded across diverse phage taxa infecting multiple bacterial hosts. These findings show that phages face a variety of bacterial defenses, driving them to evolve both specific and general strategies to overcome these barriers.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"13 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lactate production by tumor-resident Staphylococcus promotes metastatic colonization in lung adenocarcinoma 肿瘤驻留葡萄球菌产生乳酸促进肺腺癌的转移定植

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.013

Huan Yu, Yang Du, Yuling He, Yifan Sun, Junfeng Li, Bo Jia, Jiale Chen, Xinya Peng, Tongtong An, Jianjie Li, Yujia Chi, Man Wang, Lihua Cao, Yidi Tai, Xiaoyu Zhai, Reyizha Nuersulitan, Sheng Li, Nan Wu, Jia Wang, Hongchao Xiong, Ziping Wang

{"title":"Lactate production by tumor-resident Staphylococcus promotes metastatic colonization in lung adenocarcinoma","authors":"Huan Yu, Yang Du, Yuling He, Yifan Sun, Junfeng Li, Bo Jia, Jiale Chen, Xinya Peng, Tongtong An, Jianjie Li, Yujia Chi, Man Wang, Lihua Cao, Yidi Tai, Xiaoyu Zhai, Reyizha Nuersulitan, Sheng Li, Nan Wu, Jia Wang, Hongchao Xiong, Ziping Wang","doi":"10.1016/j.chom.2025.06.013","DOIUrl":"https://doi.org/10.1016/j.chom.2025.06.013","url":null,"abstract":"The role of the lung microbiota in cancer remains unclear. Here, we reveal that Staphylococcus is selectively enriched in metastatic tumor lesions and is associated with tumor recurrence in lung cancer patients. Using patient-derived bacterial strains, we employ a combination of cell line, organoid, mouse allograft, and xenograft models to demonstrate that S. nepalensis and S. capitis promote the metastatic potential of lung cancer cells. Mechanistically, lactate secreted by S. nepalensis and S. capitis upregulates MCT1 expression in tumor cells, facilitating lactate uptake and activating pseudohypoxia signaling. These effects can be eliminated by knocking out the lactate-producing genes (D-lactate dehydrogenase [ddh]/L-lactate dehydrogenase [ldh]) in the bacterial strains. Furthermore, we show that inhibiting MCT1 attenuates Staphylococcus-induced tumor metastasis both in vitro and in vivo. Collectively, our results demonstrate that tumor-resident Staphylococcus species promote lung cancer metastasis by activating host pseudohypoxia signaling and further identify key regulators as potential targets for therapeutic development.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"2 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144586954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upper vs. lower airways: Microbes shape the local milieu 上呼吸道与下呼吸道：微生物塑造了局部环境

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.007

Shifen Xu, Zhang Wang

引用次数: 0

Bugged before birth?: How maternal microbes reprogram offspring immunity 出生前就被窃听了？母体微生物如何重新编程后代免疫

IF 30.3 1区医学

Cell host & microbe Pub Date : 2025-07-09 DOI: 10.1016/j.chom.2025.06.003

Madison S. Strine, Liza Konnikova

引用次数: 0

A helping hand against severe dengue 帮助我们对抗严重的登革热