Cell host & microbe最新文献

A trivalent mucosal vaccine encoding phylogenetically inferred ancestral RBD sequences confers pan-Sarbecovirus protection in mice 编码系统发育推断出的祖先 RBD 序列的三价粘膜疫苗可为小鼠提供泛沙巴病毒保护

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-18 DOI: 10.1016/j.chom.2024.10.016

James Brett Case, Shilpa Sanapala, Carly Dillen, Victoria Rhodes, Christian Zmasek, Taras M. Chicz, Charlotte E. Switzer, Suzanne M. Scheaffer, George Georgiev, Catherine Jacob-Dolan, Blake M. Hauser, Déborah Carolina Carvalho Dos Anjos, Lucas J. Adams, Nadia Soudani, Chieh-Yu Liang, Baoling Ying, Ryan P. McNamara, Richard H. Scheuermann, Adrianus C.M. Boon, Daved H. Fremont, Michael S. Diamond

{"title":"A trivalent mucosal vaccine encoding phylogenetically inferred ancestral RBD sequences confers pan-Sarbecovirus protection in mice","authors":"James Brett Case, Shilpa Sanapala, Carly Dillen, Victoria Rhodes, Christian Zmasek, Taras M. Chicz, Charlotte E. Switzer, Suzanne M. Scheaffer, George Georgiev, Catherine Jacob-Dolan, Blake M. Hauser, Déborah Carolina Carvalho Dos Anjos, Lucas J. Adams, Nadia Soudani, Chieh-Yu Liang, Baoling Ying, Ryan P. McNamara, Richard H. Scheuermann, Adrianus C.M. Boon, Daved H. Fremont, Michael S. Diamond","doi":"10.1016/j.chom.2024.10.016","DOIUrl":"https://doi.org/10.1016/j.chom.2024.10.016","url":null,"abstract":"The continued emergence of SARS-CoV-2 variants and the threat of future Sarbecovirus zoonoses have spurred the design of vaccines that can induce broad immunity against multiple coronaviruses. Here, we use computational methods to infer ancestral phylogenetic reconstructions of receptor binding domain (RBD) sequences across multiple Sarbecovirus clades and incorporate them into a multivalent adenoviral-vectored vaccine. Mice immunized with this pan-Sarbecovirus vaccine are protected in the upper and lower respiratory tracts against infection by historical and contemporary SARS-CoV-2 variants, SARS-CoV, and pre-emergent SHC014 and Pangolin/GD coronavirus strains. Using genetic and immunological approaches, we demonstrate that vaccine-induced protection unexpectedly is conferred principally by CD4+ and CD8+ T cell-mediated anamnestic responses. Importantly, prior mRNA vaccination or SARS-CoV-2 respiratory infection does not alter the efficacy of the mucosally delivered pan-Sarbecovirus vaccine. These data highlight the promise of a phylogenetic approach for antigen and vaccine design against existing and pre-emergent Sarbecoviruses with pandemic potential.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"8 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing biosynthesized selenium nanoparticles for recruitment of beneficial soil microbes to plant roots 利用生物合成的硒纳米颗粒招募有益的土壤微生物进入植物根系

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-18 DOI: 10.1016/j.chom.2024.10.015

Chenyu Sun, Bin Sun, Lin Chen, Meilin Zhang, Pingping Lu, Mengfan Wu, Quanhong Xue, Qiao Guo, Dejian Tang, Hangxian Lai

{"title":"Harnessing biosynthesized selenium nanoparticles for recruitment of beneficial soil microbes to plant roots","authors":"Chenyu Sun, Bin Sun, Lin Chen, Meilin Zhang, Pingping Lu, Mengfan Wu, Quanhong Xue, Qiao Guo, Dejian Tang, Hangxian Lai","doi":"10.1016/j.chom.2024.10.015","DOIUrl":"https://doi.org/10.1016/j.chom.2024.10.015","url":null,"abstract":"Root exudates can benefit plant growth and health by reshaping the rhizosphere microbiome. Whether nanoparticles biosynthesized by rhizosphere microbes play a similar role in plant microbiome manipulation remains enigmatic. Herein, we collect elemental selenium nanoparticles (SeNPs) from selenobacteria associated with maize roots. In vitro and soil assays show that the SeNPs enhanced plant performance by recruiting plant growth-promoting bacteria (e.g., Bacillus) in a dose-dependent manner. Multiomic profilings unravel a cross-kingdom-signaling cascade that mediates efficient biosynthesis of SeNPs by selenobacteria. Specifically, maize roots perceive histamine signaling from Bacillus spp., which stimulates the plant to produce p-coumarate via root exudation. The rpoS gene in selenobacteria (e.g., Pseudomonas sp. ZY71) responds to p-coumarate signaling and positively regulates the biosynthesis of SeNPs. This study demonstrates a novel mechanism for recruiting host-beneficial soil microbes by microbially synthesized nanoparticles and unlocks promising possibilities for plant microbiome manipulation.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"21 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142665467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

INDELible impact: How structural variants drive virulence and resistance INDELible影响：结构变异如何驱动毒性和抗药性

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.007

Alexandra Grote

引用次数: 0

Bacterial small RNA makes a big impact for gut colonization 细菌小核糖核酸对肠道定植产生重大影响

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.010

Elena Monzel, Mahesh S. Desai

引用次数: 0

A small microcin plays a big role in V. cholerae interbacterial competition 在霍乱弧菌的细菌间竞争中，一种微小的霉素发挥着重要作用

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.011

Wei Peng, Kim Orth

引用次数: 0

If you can’t beat them, join them: Anti-CRISPR proteins derived from CRISPR-associated genes 如果你不能打败他们，那就加入他们吧：源自CRISPR相关基因的抗CRISPR蛋白

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.009

Charlie Y. Mo

引用次数: 0

mRNA vaccines: A promising platform for safer, more effective next-generation Orthopoxvirus immunization mRNA 疫苗：更安全、更有效的下一代正畸病毒免疫平台前景广阔

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.014

Xiaonan Han, Qingrui Huang, Jinghua Yan

引用次数: 0

Microbial alchemists unlock honeybee cognition 微生物炼金术士开启蜜蜂的认知能力

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.013

Huihui Sun, Guan-Hong Wang

引用次数: 0

Microbiota-induced alteration of kynurenine metabolism in macrophages drives formation of creeping fat in Crohn’s disease 微生物诱导的巨噬细胞犬尿氨酸代谢改变促使克罗恩病中爬行脂肪的形成

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.008

Jinjie Wu, Wanyi Zeng, Hongyu Xie, Mujia Cao, Jingyi Yang, Yanchun Xie, Zhanhao Luo, Zongjin Zhang, Haoyang Xu, Weidong Huang, Tingyue Zhou, Jinyu Tan, Xiaomin Wu, Zihuan Yang, Shu Zhu, Ren Mao, Zhen He, Ping Lan

{"title":"Microbiota-induced alteration of kynurenine metabolism in macrophages drives formation of creeping fat in Crohn’s disease","authors":"Jinjie Wu, Wanyi Zeng, Hongyu Xie, Mujia Cao, Jingyi Yang, Yanchun Xie, Zhanhao Luo, Zongjin Zhang, Haoyang Xu, Weidong Huang, Tingyue Zhou, Jinyu Tan, Xiaomin Wu, Zihuan Yang, Shu Zhu, Ren Mao, Zhen He, Ping Lan","doi":"10.1016/j.chom.2024.10.008","DOIUrl":"https://doi.org/10.1016/j.chom.2024.10.008","url":null,"abstract":"Hyperplasia of mesenteric tissues in Crohn’s disease, called creeping fat (CrF), is associated with surgical recurrence. Although microbiota translocation and colonization have been found in CrF, convincing mouse phenotypes and the underlying mechanisms of CrF formation remain unclear. Utilizing single-nucleus RNA (snRNA) sequencing of CrF and different mouse models, we demonstrate that the commensal Achromobacter pulmonis induces mesenteric adipogenesis through macrophage alteration. Targeted metabolome analysis reveals that L-kynurenine is the most enriched metabolite in CrF. Upregulation of indoleamine 2,3-dioxygenase 1 (IDO1) enhances kynurenine metabolism and drives mesenteric adipogenesis. Leveraging single-cell RNA (scRNA) sequencing of mouse mesenteric tissues and macrophage-specific IDO1 knockout mice, we verify the role of macrophage-sourced L-kynurenine in mesenteric adipogenesis. Mechanistically, L-kynurenine-induced adipogenesis is mediated by the aryl hydrocarbon receptors in adipocytes. Administration of an IDO1 inhibitor or bacteria engineered to degrade L-kynurenine alleviates mesenteric adipogenesis in mice. Collectively, our study demonstrates that microbiota-induced modulation of macrophage metabolism potentiates CrF formation.","PeriodicalId":9693,"journal":{"name":"Cell host & microbe","volume":"7 1","pages":""},"PeriodicalIF":30.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142601051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beta-carbolines suppress vaginal inflammation β-碳酸氢盐抑制阴道炎症

IF 30.3 1区医学

Cell host & microbe Pub Date : 2024-11-13 DOI: 10.1016/j.chom.2024.10.005

Cancan Qi, Ri-hua Xie, Yan He, Muxuan Chen

引用次数: 0