Microfluidics and Nanofluidics最新文献

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Continuous sheathless particle separation in viscoelastic fluids with different rheological properties 在具有不同流变特性的粘弹性流体中进行连续无鞘颗粒分离
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-05-08 DOI: 10.1007/s10404-024-02732-0
Chong-Shan Gan, Zhuang-Zhuang Tian, Lv Liu, Liang-Liang Fan, Liang Zhao
{"title":"Continuous sheathless particle separation in viscoelastic fluids with different rheological properties","authors":"Chong-Shan Gan,&nbsp;Zhuang-Zhuang Tian,&nbsp;Lv Liu,&nbsp;Liang-Liang Fan,&nbsp;Liang Zhao","doi":"10.1007/s10404-024-02732-0","DOIUrl":"10.1007/s10404-024-02732-0","url":null,"abstract":"<div><p>The separation of particles such as cells and bacteria in viscoelastic fluids has significant applications in biomedical fields. At present, one of the main challenges that limit the application of microfluidic technology is to separate particles in the viscoelastic fluids with different rheological properties. For instance, most existing microfluidic devices can only work in the fluid with a specific rheological property, resulting in the requirement of time-consuming design, manufacturing, testing, and optimization of different devices to separate particles in the fluids with different rheological properties. In this work, a novel hybrid three-stage microfluidic device that was made up of a micropore structure and two gradually contracted microchannels was designed to achieve efficient continuous separation of particles in the viscoelastic fluid over a wide range of rheological properties (0.07 &lt; <i>El</i> &lt; 340.41). Different separation strategies including first focusing, then initial separation, and then precise separation (FISPS) and initial separation and then precise separation (ISPS) were found. The separation strategy ISPS occurred at <i>El</i> &lt; 0.14 while the separation strategy FISPS occurred at <i>El</i> &gt; 8.43. In addition, the transformation of the separation mechanism from ISPS to FISPS was found under different flow conditions in the fluid with the transitional rheological properties (0.21 &lt; <i>El</i> &lt; 1.10). The effect of the flow rate and the rheological property of the fluid on microparticle separation were systematically studied by the experiment. With simple structure, easy operation, high separation efficiency, the present microfluidic device would have great potentials in the biomedical and clinical applications, such as the separation of cells for different patients.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140881361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction: Characterization of microfluidic trap and mixer module for rapid fluorescent tagging of microplastics 更正:用于快速荧光标记微塑料的微流体捕集器和混合器模块的特性分析
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-05-06 DOI: 10.1007/s10404-024-02731-1
Seongcheol Shin, Boeun Jeon, Wonkyu Kang, Cholong Kim, Jonghoon Choi, Sung Chul Hong, Hyun Ho Lee
{"title":"Correction: Characterization of microfluidic trap and mixer module for rapid fluorescent tagging of microplastics","authors":"Seongcheol Shin,&nbsp;Boeun Jeon,&nbsp;Wonkyu Kang,&nbsp;Cholong Kim,&nbsp;Jonghoon Choi,&nbsp;Sung Chul Hong,&nbsp;Hyun Ho Lee","doi":"10.1007/s10404-024-02731-1","DOIUrl":"10.1007/s10404-024-02731-1","url":null,"abstract":"","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140881424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A look-up table protocol for calibrating standing SAW acoustofluidics 用于校准驻留声表面波声流体的查找表协议
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-05-05 DOI: 10.1007/s10404-024-02729-9
Zixing Liu, Haixiang Zheng, Qinran Wei, Zeyi Wang, Yu Zhang, Dong Zhang, Xiasheng Guo
{"title":"A look-up table protocol for calibrating standing SAW acoustofluidics","authors":"Zixing Liu,&nbsp;Haixiang Zheng,&nbsp;Qinran Wei,&nbsp;Zeyi Wang,&nbsp;Yu Zhang,&nbsp;Dong Zhang,&nbsp;Xiasheng Guo","doi":"10.1007/s10404-024-02729-9","DOIUrl":"10.1007/s10404-024-02729-9","url":null,"abstract":"<div><p>The acoustic radiation force (ARF) acting on particles measures the performance of microfluidic devices driven by standing surface acoustic waves (SSAWs). However, existing ARF calibration techniques rely on image post-processing or additional equipment. This work proposes a look-up table method to determine the ARF by examining the particle acoustophoresis mode in discrete phase-modulated SSAW fields, where the phase difference between the two counter-propagating SAWs is changed at fixed time intervals. Theoretical analysis indicates that particles in a straight channel migrate laterally either in the “locked” mode or the “drift” mode, while mode switching can be observed when the interval reaches a critical value highly dependent on the ARF amplitude. A look-up table can then be established for a given SSAW device. By observing the particle acoustophoresis modes at different phase-changing intervals, the ARF amplitude can be obtained from the easily determined critical interval. The procedure is demonstrated experimentally in an SSAW acoustofluidic device and compared with the particle tracking protocol to verify the former’s effectiveness and demonstrate its operational simplicity. Inspired by the established theory, a method to improve the efficiency of particle acoustophoresis by optimizing the phase-modulating parameters is also proposed.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140881751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A magnetic bead array-based fluorescence immunoassay platform for robust cancer biomarker analysis in clinical blood samples 基于磁珠阵列的荧光免疫分析平台,用于临床血液样本中癌症生物标记物的可靠分析
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-29 DOI: 10.1007/s10404-024-02728-w
Jieung Oh, Ho-Seong Han, Hyundoo Hwang, Joo H. Kang
{"title":"A magnetic bead array-based fluorescence immunoassay platform for robust cancer biomarker analysis in clinical blood samples","authors":"Jieung Oh,&nbsp;Ho-Seong Han,&nbsp;Hyundoo Hwang,&nbsp;Joo H. Kang","doi":"10.1007/s10404-024-02728-w","DOIUrl":"10.1007/s10404-024-02728-w","url":null,"abstract":"<div><p>The reliable conjugation of antibodies to a solid matrix is crucial for robust immunoassays in microfluidic devices. Various magnetic particles (MPs) have been employed due to their high surface-to-volume ratios and ease of magnetic manipulation, providing a reliable surface for antibody immobilization. However, achieving uniform positioning of MPs across the channel surface has been challenging due to inadequate magnetic forces or magnetic field uniformity. Here, we present the utilization of Halbach magnetic arrays to enable consistent deployment of MPs on the microfluidic surface, thereby facilitating robust immunoassay capabilities. Using finite element method magnetics (FEMM) simulations, we predicted that incorporating Halbach magnetic arrays beneath the microfluidic channels would create more uniform and augmented magnetic flux density gradients over the surface. Subsequently, we applied this platform to assess cancer biomarkers in patients’ blood plasma and achieved statistically reliable results, comparable to those obtained using an FDA-approved device. We detected three cancer biomarkers, including prostate-specific antigen (PSA), alpha-fetoprotein (AFP), and carcinoembryonic antigen (CEA). The limit of detection (LOD) of three biomarkers were &lt; 1 ng/mL, ranging from 0.38–0.95 ng mL<sup>−1</sup>. This platform provided within-run, between-run, and between-day precisions for the three cancer biomarkers, ranging from 0.37–9.87%. This advancement holds significant promise for improving the accuracy and performance of immunoassays in various microfluidic diagnostic applications.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140826987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The dynamics of vertical coalescence of acoustically levitated droplets 声学悬浮液滴的垂直凝聚动力学
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-29 DOI: 10.1007/s10404-024-02730-2
Aditya Vashi, Ajeet Singh Yadav, Nam-Trung Nguyen, Kamalalayam Rajan Sreejith
{"title":"The dynamics of vertical coalescence of acoustically levitated droplets","authors":"Aditya Vashi,&nbsp;Ajeet Singh Yadav,&nbsp;Nam-Trung Nguyen,&nbsp;Kamalalayam Rajan Sreejith","doi":"10.1007/s10404-024-02730-2","DOIUrl":"10.1007/s10404-024-02730-2","url":null,"abstract":"<div><p>Mobility manipulation of liquid droplets is an important task of digital imicrofluidics. Acoustic levitation has revolutionised the contactless manipulation of liquid droplets for various applications. Acoustic levitation technique can be effectively used to manipulate droplets to obtain their coalescence. This paper reports a unique, versatile, and material-independent approach for the vertical coalescence of the droplets suspended in an acoustic levitator. The acoustic power of the levitator is carefully engineered to obtain vertical coalescence of two liquid droplets. Water, 20% and 40% glycerol–water solutions are used as the working liquids. The results of the experiments revealed three outcomes during the coalescence. The outcomes are analysed and discussed.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10404-024-02730-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transonic, supersonic, and hypersonic flow of rarefied gas into vacuum through channels with a forward- or backward-facing step 稀薄气体以跨音速、超音速和高超音速流经带有前向或后向台阶的通道进入真空
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-25 DOI: 10.1007/s10404-024-02727-x
O. Sazhin, A. Sazhin
{"title":"Transonic, supersonic, and hypersonic flow of rarefied gas into vacuum through channels with a forward- or backward-facing step","authors":"O. Sazhin,&nbsp;A. Sazhin","doi":"10.1007/s10404-024-02727-x","DOIUrl":"10.1007/s10404-024-02727-x","url":null,"abstract":"<div><p>Numerical investigations of high-speed rarefied gas outflow into a vacuum through channels with a forward- or backward-facing step have been conducted using the direct simulation Monte Carlo method. Calculations have been performed for various free-stream Mach numbers, covering transonic, supersonic, and hypersonic flow regimes, and over a wide range of gas rarefaction from free molecular to near hydrodynamic conditions. Mass flow rates through the channel and the gas flow field have been accurately calculated both inside the channel and in the regions upstream and downstream. It has been established that channel geometry, the free-stream velocity, and gas rarefaction strongly influence the gas flow. In the flow field, in front of the channel, a phenomenon known as a detached shock occurs, while inside the channel, a gas recirculation zone may form.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140655043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A 3D printed acoustofluidic nozzle-diffuser microfluidic pump 三维打印声学流体喷嘴-扩散器微流体泵
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-15 DOI: 10.1007/s10404-024-02722-2
Erturan Yetiskin, Sinan Gucluer, Ilayda Erdem, Adem Ozcelik
{"title":"A 3D printed acoustofluidic nozzle-diffuser microfluidic pump","authors":"Erturan Yetiskin,&nbsp;Sinan Gucluer,&nbsp;Ilayda Erdem,&nbsp;Adem Ozcelik","doi":"10.1007/s10404-024-02722-2","DOIUrl":"10.1007/s10404-024-02722-2","url":null,"abstract":"<div><p>Microfluidic flow control systems are critical components for on-chip biomedical applications. This study introduces a new micropump for on-chip sample preparation and analysis by using an acoustic nozzle diffuser mechanism. The micropump implements a commercially available transducer and control board kit with 3D-printed fluid reservoirs. In this micropump, conic-shaped micro-holes on the metal sheet cover of the transducer are employed as oscillating nozzle diffuser micro arrays to achieve directional flow control. The micropump is shown to efficiently pump water and particle mixtures exceeding flow rates of 515 µl/min at a 12-volt input voltage. In addition, owing to the small size of the nozzle hole opening, larger particles can also be filtered out from a sample solution during fluid pumping enabling a new function. Importantly, the micropump can be fabricated and assembled without needing a cleanroom, making it more accessible. This feature is advantageous for researchers and practitioners, eliminating a significant barrier to entry. By combining commercially available components with 3D printing technology, this micropump presents a cost-effective and versatile solution for on-chip applications in biomedical research and analysis.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10404-024-02722-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fluid lubrication model over sinusoidal roughness with streamline-based approach 基于流线的正弦粗糙度流体润滑模型
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-15 DOI: 10.1007/s10404-024-02721-3
Haruki Okazaki, Shintaro Takeuchi
{"title":"Fluid lubrication model over sinusoidal roughness with streamline-based approach","authors":"Haruki Okazaki,&nbsp;Shintaro Takeuchi","doi":"10.1007/s10404-024-02721-3","DOIUrl":"10.1007/s10404-024-02721-3","url":null,"abstract":"<div><p>A method is proposed to deterministically obtain steady lubrication pressure for the Stokes flow in a channel bounded by a flat wall and a surface with roughness represented by sinusoidal waves. A streamline sufficiently far away from the rough surface is used to formulate a streamline-based lubrication equation with the velocity on the streamline, and the velocities on the streamline is imposed as a boundary condition. In the solution of the lubrication equation, by virtually moving the streamline towards the flat wall, the pressure on the flat wall is obtained, and then the wall-normal variation of the pressure is recovered from the wall pressure by a lubrication model that considers higher order terms. The proposed method is applied to lubrication flows in channels with roughness represented by a single sinusoidal wave and a superposition of several sinusoidal waves. Through comparison with analytical solutions, the validity of the proposed method is established, and the applicable range of superposition of waves is explained that lowest-wavenumber component in surface profile is sufficiently isolated from higher-wavenumber components. Although the problem setting intrinsically prohibits the application of the conventional Reynolds lubrication equation, this study provides new understandings for the pressure obeying the Reynolds lubrication equation and the role of the higher-order terms.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Merged and alternating droplets generation in double T-junction microchannels using symmetrically inserted capillaries 利用对称插入的毛细管在双 T 型交界微通道中生成合并和交替液滴
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-14 DOI: 10.1007/s10404-024-02725-z
Feng Shen, Yuedong Zhang, Chunyou Li, Yan Pang, Zhaomiao Liu
{"title":"Merged and alternating droplets generation in double T-junction microchannels using symmetrically inserted capillaries","authors":"Feng Shen,&nbsp;Yuedong Zhang,&nbsp;Chunyou Li,&nbsp;Yan Pang,&nbsp;Zhaomiao Liu","doi":"10.1007/s10404-024-02725-z","DOIUrl":"10.1007/s10404-024-02725-z","url":null,"abstract":"<p>In this work, merged and alternating droplets generated in a microfluidic double T-junction are investigated using experiments and numerical simulations. The double T-junction is constructed by symmetrically inserting two capillaries into a microfluidic chip at specific positions. We explore the effects of the two-phase flow rate fraction, capillary tip distance (30 μm, 60 μm, and 200 μm), and fluid properties on droplet formation phenomena. Detailed observations reveal four distinct regimes during the dynamic evolution of the two-phase interface morphology: merged state, stable alternating droplets, droplet pairs, and jetting. Two phase diagrams are obtained to demonstrate that interfacial tension and dispersed phase viscosity significantly influence these regimes. Moreover, we find that as the flow rate fraction increases from 0.054 to 0.286, the length of generated droplets increases from 156 to 789 μm; we provide a theoretical prediction formula for dimensionless droplet length accordingly. Additionally, our simulations show fluctuating pressure in dispersed flows throughout the process of droplet generation. The simulated pressure in the dispersed flows fluctuates during the droplet generation process. The understanding of the underlying physics of the capillary-based double T-junction contributes valuable insights for various related applications.</p>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploring the stability of single emulsion created by microfluidics and its use in the production of core–shell microparticles 探索微流控技术产生的单一乳液的稳定性及其在核壳微颗粒生产中的应用
IF 2.3 4区 工程技术
Microfluidics and Nanofluidics Pub Date : 2024-04-08 DOI: 10.1007/s10404-024-02723-1
Mehrnaz Oveysi, Vahid Bazargan, Amir Nejat, Marco Marengo
{"title":"Exploring the stability of single emulsion created by microfluidics and its use in the production of core–shell microparticles","authors":"Mehrnaz Oveysi,&nbsp;Vahid Bazargan,&nbsp;Amir Nejat,&nbsp;Marco Marengo","doi":"10.1007/s10404-024-02723-1","DOIUrl":"10.1007/s10404-024-02723-1","url":null,"abstract":"<div><p>This study introduces an innovative method aimed at achieving exceptional stability in emulsions. The primary focus is on re-emulsifying precisely controlled and uniform initial single emulsions, generated by microfluidic devices, to produce single-core double emulsions and core–shell microparticles. Departing from traditional approaches, our method employs a unique combination of advanced Two-level fractional factorial design and numerical simulation. These tools are utilized to discern and optimize critical parameters necessary for the formation of highly monodispersed stable single emulsions and their subsequent transformation into double emulsions. Correlations are established to estimate the size and stability of the primary single emulsion based on immiscible phase flow rate ratio and surfactant concentration. These correlations provide a comprehensive understanding that facilitates the intentional development of desired water-in-oil emulsions. The proposed microfluidic paradigm shows promise for the controlled and efficient production of single-core double emulsions, with broad applications in Pharmaceuticals, Food, and Cosmetics.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 5","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140571067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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