Microfluidics and Nanofluidics最新文献_第5页

Parametric study on the influence of varying angled inlet channels on mixing performance in simple T micromixers and vortex T micromixers across a wide range of Reynolds numbers 关于不同角度的入口通道对简单 T 型微搅拌器和涡旋 T 型微搅拌器在宽雷诺数范围内的搅拌性能影响的参数研究

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-07-08 DOI: 10.1007/s10404-024-02746-8

Kamran Rasheed, Mubashshir Ahmad Ansari, Shahnwaz Alam, Mohammad Nawaz Khan, Mahmood Alam

{"title":"Parametric study on the influence of varying angled inlet channels on mixing performance in simple T micromixers and vortex T micromixers across a wide range of Reynolds numbers","authors":"Kamran Rasheed, Mubashshir Ahmad Ansari, Shahnwaz Alam, Mohammad Nawaz Khan, Mahmood Alam","doi":"10.1007/s10404-024-02746-8","DOIUrl":"10.1007/s10404-024-02746-8","url":null,"abstract":"<div><p>Micromixers become the core elements of lab-on-chip (LOC) devices used for mixing fluid samples at a very small scale. For modest Reynolds numbers, the nature of fluid movement is laminar across the microchannel hence mixing is challenging. Numerous designs of micromixers for mixing enhancement inside microfluidic devices have been developed to solve this issue. The current investigation looks at the performance of two distinct versions of passive micromixers i.e. simple T micromixer (STMM) and vortex T micromixer (VTMM), employing different angular configurations (i.e. 30°, 60°, 90°, 120° and 150°) on their inlet channel to monitor the consistency of blending for the Reynolds number in a range of 10–150. Numerical investigations were done by performing simulations on these geometrical arrangements to evaluate the level of mixing, pressure gradient and cost of mixing. The outcome indicates the performance of mixing is dependent on the angular arrangement of inlet channels. For STMM, the layout with inlet channels at 120° performs most effectively, whereas, for VTMM, the configuration with inlets at 90° performs best.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141576014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrically actuated peristaltic transport of viscoelastic fluid: a theoretical analysis 粘弹性流体的电动蠕动输送：理论分析

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-07-08 DOI: 10.1007/s10404-024-02742-y

Mahesh Kumar, Pranab Kumar Mondal

{"title":"Electrically actuated peristaltic transport of viscoelastic fluid: a theoretical analysis","authors":"Mahesh Kumar, Pranab Kumar Mondal","doi":"10.1007/s10404-024-02742-y","DOIUrl":"10.1007/s10404-024-02742-y","url":null,"abstract":"<div><p>In this article, we discuss the bioinspired peristaltic pumping mechanism of an elastic non-Newtonian fluid whose rheology is characterized by the Phan-Thien-Tanner model in a microfluidic configuration. We consider the effect of an electroosmotic body force originating from electrical double layer phenomena formed in the wall of the fluidic channel of finite length. The considered configuration is consistent with the natural contraction of the oesophagus wall that does not involve expansion beyond the stationary boundary. Employing lubrication theory and assuming the underlying flow to be in the creeping flow regime, we outline the transport equations pertaining to the chosen peristaltic set up. The transport equations are then solved using a well-established method consistent with perturbation technique. By depicting the pressure variation and wall shear stress graphically for a continuous wave train, we aptly discuss the time-averaged net throughput and flow developed at channel inlet of the chosen pathway and demonstrate the eventual consequences of these flow patterns for a window of viscoelastic and electrokinetic parameters. The outcomes obtained from this model establishes that the underlying flow owing to the peristaltic pumping mechanism strongly relies on the rheological parameter <span>(varepsilon W{e}^{2})</span>. These inferences are expected to be of extensive importance in designing peristalsis pump, mimicking features of the physiological system, for achieving unidirectional flow of complex fluids with improved efficiency, frequently used in biochemical/biomicrofluidic applications.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141576013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Machine learning-based bead enumeration in microfluidics droplets enhances the reliability of monitoring bead encapsulation toward single-cell sorting applications 微流控液滴中基于机器学习的微珠计数提高了单细胞分拣应用中监测微珠封装的可靠性

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-07-06 DOI: 10.1007/s10404-024-02748-6

Hoang Anh Phan, Nguyen Dang Pham, Loc Quang Do, Tung Thanh Bui, Hai Hoang Nguyen, Trinh Duc Chu

{"title":"Machine learning-based bead enumeration in microfluidics droplets enhances the reliability of monitoring bead encapsulation toward single-cell sorting applications","authors":"Hoang Anh Phan, Nguyen Dang Pham, Loc Quang Do, Tung Thanh Bui, Hai Hoang Nguyen, Trinh Duc Chu","doi":"10.1007/s10404-024-02748-6","DOIUrl":"10.1007/s10404-024-02748-6","url":null,"abstract":"<div><p>The encapsulation of cells within droplets is a crucial aspect of various cell analysis applications. Current research has focused on accurately detecting and identifying cell types or cell counts within droplets using object detection in bright-field images. However, there are only a few in-depth investigations into the impact of the image data quality acquired from optical systems on computer vision models. This study examines several popular machine learning object detection models to analyze scenarios complicating the identification of bead locations within a droplet, posing challenges for computer vision models. A microfluidic droplet generation system was developed and implemented, coupled with optical devices to capture images of encapsulated beads within the droplet. To identify the most efficient model, a specific dataset was meticulously selected from the overall data, encompassing images depicting overlapping beads and edge-drifting scenarios. The proposed method achieved up to 98.2% accuracy on the testing dataset and 95% in real-time testing with the YOLOv8 model, enhancing bead count precision within droplets and clarifying the correlation between accuracy and frame recognition thresholds. This work holds particular importance in single-cell sorting, where precision is critical in ensuring meaningful outcomes, particularly concerning rare cell types such as cancer cells.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141546533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reversible, stable and uniform SERS in a Y-shaped microfluidic chip: chemical imaging of concentration gradients Y 形微流体芯片中可逆、稳定和均匀的 SERS：浓度梯度的化学成像

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-07-03 DOI: 10.1007/s10404-024-02740-0

Fabien Chauvet

{"title":"Reversible, stable and uniform SERS in a Y-shaped microfluidic chip: chemical imaging of concentration gradients","authors":"Fabien Chauvet","doi":"10.1007/s10404-024-02740-0","DOIUrl":"10.1007/s10404-024-02740-0","url":null,"abstract":"<div><p>Imaging of chemical composition in microfluidic chips is addressed using Surface Enhanced Raman Spectroscopy (SERS). The Y-shaped SERS microfluidic chip used is fabricated by xurography and an electrodeposition method is employed to form a thin nanostructured silver layer over the bottom glass wall of the main microchannel. Used as an immobilized SERS substrate, this layer of silver nanocrystals exhibits an analytical enhancement factor of 5.10<span>(^4)</span> uniformly distributed over its surface (RSD < 7%). These good performances allow the quantitative imaging of transverse diffusion profiles of Crystal Violet (CV) at low concentrations (<span>(10^{-8}-10^{-6})</span> mol/L). The SERS measurement turns out to be reversible at high laser power and this is explained by the thermal desorption of adsorbed CV (photothermal effect). However, too high heating leads to a low amount of adsorbed species and a low SERS signal. This effect is limited by using a fast enough flow inducing a cooling effect. A compromise must be found between laser power and liquid flow rate to enable a reversible and sensitive SERS measurement in the chip. These findings should contribute to the development of imaging, in microfluidic conditions, of the spatiotemporal dynamics of weakly concentrated key molecules involved in chemical, biochemical or biological processes.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 8","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141531413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A low-cost method of fabricating Parylene-SU-8 micro-nanofluidic chip by thermal nanoimprint and multilayer compositing 利用热纳米压印和多层复合技术制造聚对二甲苯-SU-8 微纳流体芯片的低成本方法

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-06-28 DOI: 10.1007/s10404-024-02743-x

Lei Sun, Zhifu Yin, Ran Guo

引用次数: 0

Lung-on-a-chip composed of styrene-butadiene-styrene nano-fiber/porous PDMS composite membranes with cyclic triaxial stimulation 由苯乙烯-丁二烯-苯乙烯纳米纤维/多孔 PDMS 复合膜组成的肺芯片受到循环三轴刺激

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-06-24 DOI: 10.1007/s10404-024-02739-7

Yuru You, Changling Zhang, Zhixiang Guo, Feng Xu, Daoheng Sun, Junjie Xia, Songyue Chen

{"title":"Lung-on-a-chip composed of styrene-butadiene-styrene nano-fiber/porous PDMS composite membranes with cyclic triaxial stimulation","authors":"Yuru You, Changling Zhang, Zhixiang Guo, Feng Xu, Daoheng Sun, Junjie Xia, Songyue Chen","doi":"10.1007/s10404-024-02739-7","DOIUrl":"10.1007/s10404-024-02739-7","url":null,"abstract":"<div><p>The physiological function of lung is strongly correlated with its unique structural microenvironment and mechanical stimulation. Most existing lung-on-a-chips (LOCs) do not replicate the key physiological structure and stimulation of human lung, reducing their reliability in application. In this study, a scaffold structure of a styrene-butadiene-styrene (SBS) nanofiber and porous honeycomb polydime-thylsiloxane (PDMS) composite membrane was developed to construct an alveolar air-blood barrier that mimics the alveolar characteristics of flexibility, cross-scale structure, and triaxial mechanical stimulation. By combining micro-fluidic and electrospinning technology, a biomimetic LOC with dynamic triaxial cyclic strain was realized. The composite membrane had a Young’s modulus of 0.54 ± 0.05 MPa and was capable of 8–12% strain at 1 kPa air pressure. We monocultured and co-cultured human non-small cell lung cancer cells stably expressing red fluorescent protein (A549-RFP) with human umbilical vein endothelial cell stably expressing green fluorescent protein (HUVECs-GFP) within the chip. A multi-layered structure of epithelial cell layer-basal layer-endothelial cell layer, similar to the air-blood barrier in vivo, was constructed. The LOC was proved to be an initial foundation for creating in vitro alveolar physiological models, and could be a potential platform for application in physiology, pathology, toxicology, drug screening, and customized medicine.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141502840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in modeling permeability and selectivity of the blood-brain barrier using microfluidics 利用微流体技术建立血脑屏障渗透性和选择性模型的进展

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-06-23 DOI: 10.1007/s10404-024-02741-z

Jindi Sun, Shang Song

{"title":"Advances in modeling permeability and selectivity of the blood-brain barrier using microfluidics","authors":"Jindi Sun, Shang Song","doi":"10.1007/s10404-024-02741-z","DOIUrl":"10.1007/s10404-024-02741-z","url":null,"abstract":"<div><p>The blood-brain barrier (BBB) protects the brain by actively allowing the entry of ions and nutrients while limiting the passage of from toxins and pathogens. A healthy BBB has low permeability and high selectivity to maintain normal brain functions. Increased BBB permeability can result from neurological diseases and traumatic injuries. Modern engineering technologies such as microfluidics and fabrication techniques have advanced the development of BBB models to simulate the basic functions of BBB. However, the intrinsic BBB properties are difficult to replicate. Existing <i>in vitro</i> BBB models demonstrate inconsistent BBB permeability and selectivity due to variations in microfluidic design, cell types and arrangement, expression of tight junction (TJ) proteins, and use of shear stress. Specifically, microfluidic designs have flow channels of different sizes, complexity, topology, and modular structure. Different cell types are selected to mimic various physiological conditions. These factors make it challenging to compare results obtained using different experimental setups. This paper highlights key factors that play important roles in influencing microfluidic models and discusses how these factors contribute to permeability and selectivity of the BBB models.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141502841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Finger-operated pumping platform for microfluidic preparation of nanoparticles 用于微流控制备纳米粒子的指控泵平台

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-06-14 DOI: 10.1007/s10404-024-02738-8

Ahmed Azmeer, Ibraheem Kanan, Ghaleb A. Husseini, Mohamed Abdelgawad

{"title":"Finger-operated pumping platform for microfluidic preparation of nanoparticles","authors":"Ahmed Azmeer, Ibraheem Kanan, Ghaleb A. Husseini, Mohamed Abdelgawad","doi":"10.1007/s10404-024-02738-8","DOIUrl":"10.1007/s10404-024-02738-8","url":null,"abstract":"<div><p>Microfluidic preparation of nanoparticles (NPs) offers many advantages over traditional bench-top preparation techniques, including better control over particle size and higher uniformity. Although many studies have reported the use of low-cost microfluidic chips for nanoparticle synthesis, the technology is still expensive due to the high cost of the pumps needed to generate the required flows inside microchannels. Here, we present a low-cost finger-operated constant-pressure pumping platform capable of generating pressures as high as 120 kPa using finger-operated pumping caps that can be attached to any pop bottle. The platform costs around $208 and enables the generation of flow rate ratios (FRR) of up to 47:1 for the continuous flow synthesis of NPs. The pump has a resolution of 500 Pa per stroke and exhibits stable pressures for up to a few hours. To show the functionality of the proposed pump, we used it to prepare pegylated liposomes and poly lactic-co-glycolic acid (PLGA) nanoparticles with sizes ranging from 47 nm to 250 nm with an average polydispersity of 20% using commercially available micromixer chips and in-house made hydrodynamic flow focusing devices. We believe this platform will render microfluidic preparation of NPs accessible to any laboratory with minimal capabilities.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141341822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment 在微流体平台上设计用于细胞排列的独立电纺 PLLCL 纤维

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-06-07 DOI: 10.1007/s10404-024-02736-w

Özüm Yildirim-Semerci, Ahu Arslan-Yildiz

{"title":"Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment","authors":"Özüm Yildirim-Semerci, Ahu Arslan-Yildiz","doi":"10.1007/s10404-024-02736-w","DOIUrl":"10.1007/s10404-024-02736-w","url":null,"abstract":"<div><p>Here, a PLLCL-on-chip platform was developed by direct electrospinning of poly (L-lactide-co-ε-caprolactone) (PLLCL) on polymethyl methacrylate (PMMA) microfluidic chips. Designed microchip provides the electrospinning of free-standing aligned PLLCL fibers which eliminates limitations of conventional electrospinning. Besides, aligned fiber structure favors cell alignment through contactless manipulation. Average fiber diameter, and fiber alignment was evaluated by SEM analyses, then, leakage profile of microchip was investigated. 3D cell culture studies were conducted using HeLa and NIH-3T3 cells, and nearly 85% cell viability was observed in PLLCL-on-chip for 15 days, while cell viability of 2D control started to decrease after 7 days based on Live dead and Alamar Blue analyses. These findings emphasize biocompatibility of PLLCL-on-chip platform for 3D cell culture and its ability to mimic extracellular matrix (ECM). Immunostaining results prove that PLLCL-on-chip platform favors the secretion of ECM proteins compared to control groups, and cytoskeletons of cells were in aligned orientation in PLLCL-on-chip, while they were in random orientation in control groups. Overall, these results demonstrate that the developed platform is suitable for the formation of various 3D cell culture models and a potential candidate for cell alignment studies.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10404-024-02736-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141371849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simulation analysis and experimental verification of thermodynamic characteristics of integrated droplet digital PCR chip 集成液滴数字 PCR 芯片热力学特性的仿真分析与实验验证

IF 2.3 4区工程技术

Microfluidics and Nanofluidics Pub Date : 2024-06-04 DOI: 10.1007/s10404-024-02737-9

Xiangkai Meng, Luyang Duanmu, Ping Gong

{"title":"Simulation analysis and experimental verification of thermodynamic characteristics of integrated droplet digital PCR chip","authors":"Xiangkai Meng, Luyang Duanmu, Ping Gong","doi":"10.1007/s10404-024-02737-9","DOIUrl":"10.1007/s10404-024-02737-9","url":null,"abstract":"<div><p>In order to reduce the influence of the thermal conductivity of the digital polymerase chain reaction (dPCR) chip material and the temperature distribution of the droplet collection chamber on the amplification effect, an optimized integrated dPCR chip was designed. The heat conduction of the designed dPCR gene chip was simulated by COMSOL finite element model, which provided theoretical basis for the design and fabrication of the chip. Three-dimensional ht models of dPCR microarray under steady state and transient conditions were established. The thermodynamic simulation of dPCR gene chip was carried out by changing the material, thickness, structure and width of droplet collection chamber. During the high temperature denaturation stage of amplification, the temperature characteristics were analyzed, and the surface temperature, heating curve, isotherm, thermal expansion and other results of the dPCR gene chip were obtained, and the structural parameters of the chip design were optimized to provide guidance for the subsequent chip design. The results showed that the internal temperature uniformity of the COC sample was higher than other materials. The chip has a thickness of 2 mm and the collection chamber has a width of 4 mm, which was better suited to meet the requirements of PCR reaction. The PCR amplification device was established, and the uniformity of temperature distribution of the fabricatedchip was verified by thermal imager. The results showed that the heat conduction speed was fast, the heat conduction was uniform, and the uniformity was less than ± 0.5 °C. Therefore, under the premise of meeting the quantity of microdroplet generation, the chip designed in this paper has excellent heat conduction performance.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":"28 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141258571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0