Doklady Biochemistry and Biophysics最新文献_第4页

Prebiotic Properties of Structurally Diverse Plant Polysaccharides 结构多样植物多糖的益生元特性

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601428

M. V. Kharina, A. A. Mikhailova, P. V. Mikshina

{"title":"Prebiotic Properties of Structurally Diverse Plant Polysaccharides","authors":"M. V. Kharina, A. A. Mikhailova, P. V. Mikshina","doi":"10.1134/S1607672925601428","DOIUrl":"10.1134/S1607672925601428","url":null,"abstract":"This study is aimed to evaluate the prebiotic potential of plant polysaccharides of various structural types and origins, including acacia gum arabinogalactan, beech wood xylan, apple pectin enriched in homogalacturonan, flaxseed mucilage (containing arabinoxylan, and rhamnogalacturonan I), and chicory inulin. All carbohydrate samples varied in their monosaccharide composition and molecular weight distribution. The prebiotic activity of the polysaccharides was assessed using in vitro models under anaerobic conditions with the Bifidobacterium bifidum 791 and Lactobacillus acidophilus n.v. Ep 317/402. It was found that adding various polysaccharides to the nutrient media intensified lactic acid production, increased the biomass yield of bifidobacteria and lactobacilli, and influenced the rate and extent of substrate assimilation. The lower molecular weight inulin (4.2 kDa) and arabinogalactan (115 kDa) supported higher biomass yields in both strains. The viable cell count of Bifidobacterium bifidum 791 (colony-forming units per mL) increased by 3.5, 12.3, 110.5, 128.1, and 215.8% compared to the control upon the addition of beech wood xylan, flaxseed mucilage polysaccharides, apple pectin, inulin, and acacia arabinogalactan, respectively. For L. acidophilus n.v. Ep 317/402, the addition of the same polysaccharides increased the biomass yield by 1.5, 7.7, 64.6, 69.2, and 207.7%, respectively, versus the control. Flaxseed mucilage, composed of rhamnogalacturonan I (3400 kDa) and arabinoxylan (1330 kDa), supported a high biomass yield in both cultures. Based on this research, it can be concluded that flaxseed mucilage promoted probiotic growth in a manner similar to, and often more effectively than, the established prebiotic inulin. Due to the availability of flaxseeds, simple mucilage extraction process, and the limited existing research, flaxseed polysaccharide complex emerges as a promising candidate for further development as a prebiotic supplement.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 2","pages":"644 - 652"},"PeriodicalIF":0.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Master-Switches of Secondary Cell Wall Formation in Flax (Linum usitatissimum L.) 亚麻次生细胞壁形成的主开关

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601295

N. Mokshina, N. Syrchina, T. Gorshkova

{"title":"Master-Switches of Secondary Cell Wall Formation in Flax (Linum usitatissimum L.)","authors":"N. Mokshina, N. Syrchina, T. Gorshkova","doi":"10.1134/S1607672925601295","DOIUrl":"10.1134/S1607672925601295","url":null,"abstract":"All vital processes in plants are regulated by a complex network of transcription factors. The formation of the cell wall, one of the key structures of plant cells that determines plant development and function, is tightly controlled. In particular, the main target genes regulated by transcription factors encode enzymes involved in polysaccharide metabolism, specifically their biosynthesis and modification. The transcriptional network governing secondary cell wall formation comprises the bulk of vascular and mechanical tissues, which together constitute a general component of wood. Despite overlapping main components, secondary cell walls in fibers and vessels are controlled by distinct master regulators. Flax phloem fibers form a tertiary cell wall composed mainly of cellulose and a small portion of pectins, with an absence of xylan and lignin. Formation of the tertiary cell wall requires the deposition of even a thin layer of secondary cell wall. For the first time, a comprehensive revision of VNS genes, belonging to the NAC family of transcription factors, has been performed in flax. The NST group of NAC transcription factors, potentially involved in secondary cell wall formation in both phloem and xylem fibers, has been identified. These genes represent valuable targets for both fundamental studies on cell wall biogenesis and molecular-genetic manipulation aimed at improving plant quality.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 2","pages":"606 - 613"},"PeriodicalIF":0.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and Characterization of Halophilic Bacteria of the Halomonadaceae Family, Promising Producers of Extracellular Polysaccharides 盐单胞菌科嗜盐菌的分离与特性研究

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601441

Yu. P. Fedonenko, V. S. Grinev, N. S. Velichko, N. N. Lipatov, N. Yu. Selivanov, M. S. Kuzina, E. N. Sigida, S. A. Konnova

{"title":"Isolation and Characterization of Halophilic Bacteria of the Halomonadaceae Family, Promising Producers of Extracellular Polysaccharides","authors":"Yu. P. Fedonenko, V. S. Grinev, N. S. Velichko, N. N. Lipatov, N. Yu. Selivanov, M. S. Kuzina, E. N. Sigida, S. A. Konnova","doi":"10.1134/S1607672925601441","DOIUrl":"10.1134/S1607672925601441","url":null,"abstract":"Exopolysaccharide (EPS) production is one of the key mechanisms of bacterial survival in hypermineralized environments. Representatives of the Halomonadaceae family are recognized as basic organisms for next-generation industrial biotechnology; however, the range of Halomonas used and information on the structural diversity of their EPSs are rather limited. The results of metagenomic analysis of salt, peloid, and soil samples from the coastal zone of salt lakes in the Volgograd region with subsequent seeding on selective mineral media made it possible to isolate bacteria of the Halomonadaceae family. For taxonomically identified strains, cultivation conditions were optimized and EPSs were obtained, the structure of which was characterized based on chemical analysis data and NMR spectroscopy. The study of the physicochemical properties of EPSs (viscosity, hygroscopicity, emulsifying activity, and antioxidant properties) showed that these polymers are promising for biotechnological applications.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 2","pages":"653 - 658"},"PeriodicalIF":0.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interaction of Human Peripheral NK Cells with Bacterial Polysaccharides 人外周血NK细胞与细菌多糖的相互作用

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601519

A. A. Boyko, L. M. Kanevskiy, E. M. Rapoport, A. B. Tuzikov, N. V. Shilova, E. I. Kovalenko

引用次数: 0

Structural Features of Different Functional Types of Flax Rhamnogalacturonan I 亚麻不同功能类型鼠李糖半乳草酸酯的结构特征

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601313

P. Mikshina, I. Shaikhieva, O. Korobkina, A. Mikhailova, M. Kharina, T. Sibgatullin

{"title":"Structural Features of Different Functional Types of Flax Rhamnogalacturonan I","authors":"P. Mikshina, I. Shaikhieva, O. Korobkina, A. Mikhailova, M. Kharina, T. Sibgatullin","doi":"10.1134/S1607672925601313","DOIUrl":"10.1134/S1607672925601313","url":null,"abstract":"Rhamnogalacturonans I (RGs-I) are complex, multifunctional pectic polysaccharides found in plant cell walls and seed mucilages. Their structure varies based on the source, tissue type, and growth stage. This study investigates the structural peculiarities of different functional types of RG-I from flax, focusing on NMR analysis of RGs-I from seed mucilage, primary cell walls of hypocotyls, and tertiary cell walls of fibers. RG-I from flaxseed mucilage features single-residue side chains of Galp and Fucp, with 55% Rhap substitution at the rare O-3 position, no homogalacturonan, and the presence of acetyl groups. Primary cell wall RG-I includes homogalacturonan (up to 50% of the total backbone), 54% Rhap substitution at O-4, and short β-(1 → 4)-galactan and α-(1 → 5)-arabinan side chains (averaging 2–3 residues), with 17% represented by single Ara and Gal units, along with acetyl, feruloyl, and benzoyl groups. The tertiary cell wall RG-I has a pure backbone without homogalacturonan and the highest Rhap substitution at O-4 (72%) by long galactan side chains (up to 92 residues). Modifications of this RG-I in the cell wall suggest the removal of acetyl groups and trimming of galactan chains (47% reduced to a single residue; longer side chains of up to 16 residues remaining). This research provides insights into the structural diversity of RG-I within a single plant, contributing to the understanding of their functional roles in plant growth and development.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 2","pages":"622 - 631"},"PeriodicalIF":0.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the Role of Membrane Environment in the Recognition of GD2 Ganglioside with Antibodies 研究膜环境在抗体识别GD2神经节苷脂中的作用

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601544

M. S. Sokolova, V. A. Zubricheva, E. M. Rapoport, D. O. Anisimova, I. I. Mikhalyov, R. V. Kholodenko, N. V. Bovin, I. M. Ryzhov

引用次数: 0

Antibiofilm Activity of Hemolymph Lectins from the Bivalve Mollusk Glycymeris yessoensis 双壳类软体动物血淋巴凝集素的抗膜活性

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601416

T. O. Mizgina, I. V. Chikalovets, O. I. Nedashkovskaya, O. V. Chernikov

引用次数: 0

Epitope Recognized by anti-A,B Antibodies of Blood Group O Individuals as Probed with STD NMR 用STD核磁共振检测O型血个体抗a、B抗体识别的表位

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601507

S. V. Tsygankova, P. S. Obukhova, T. V. Tyrtysh, A. S. Paramonov, N. V. Bovin

引用次数: 0

Glucanosyltransglycosylase Activity of the Glucan-Remodeling Enzyme Bgl2p in the Yeast Cell Wall: Detection and Characterization 酵母细胞壁葡聚糖重塑酶Bgl2p的葡聚糖基转糖基酶活性：检测和表征

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601568

T. S. Kalebina, V. V. Rekstina, N. A. Drozdov, A. P. Sinitsyn

引用次数: 0

Gene Expression Profiles of Amaranthin-Like Proteins with Distinct Domain Combinations in Flax (Linum usitatissimum) 亚麻（Linum usitatissimum）不同结构域组合紫红素样蛋白基因表达谱

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2026-01-30 DOI: 10.1134/S1607672925601325

N. Syrchina, N. Mokshina, N. Petrova

{"title":"Gene Expression Profiles of Amaranthin-Like Proteins with Distinct Domain Combinations in Flax (Linum usitatissimum)","authors":"N. Syrchina, N. Mokshina, N. Petrova","doi":"10.1134/S1607672925601325","DOIUrl":"10.1134/S1607672925601325","url":null,"abstract":"Amaranthin-like proteins are lectins with distinct domain organization, but their functions in plants remain unclear. Flax (Linum usitatissimum) has one of the largest Amaranthin protein family among plant species, with 19 members grouped into three types of domain combinations: single amaranthin domain (A), two amaranthin domains with one aerolysin domain (AAT), and one or two amaranthin domains with one Bet v 1 domain (AB/AAB). In this study, we examined the expression of 13 amaranthin-like genes. Genes encoding proteins with a single amaranthin domain were active during early development and showed temporary upregulation in phloem fibers and xylem tissues after gravistimulation, suggesting possible roles in cell wall rearrangement and biosynthesis. AB2 and AAB2 genes were mainly expressed in phloem fibers at later stages, indicating possible involvement in phloem fiber formation. AAT1 was the only gene clearly expressed during intrusive phloem fiber growth. These results reveal that the different protein domain combinations correspond to distinct, tissue- and stage-specific gene expression patterns, pointing to specialized roles in phloem fiber formation, xylem development, and cell wall dynamics, and highlight candidate genes for further functional studies.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 2","pages":"614 - 621"},"PeriodicalIF":0.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0