Doklady Biochemistry and Biophysics最新文献

{"title":"The Role of D/H Isotope Exchange in Stabilizing Trinucleotide Repeat Expansions in the CAG Tract of the ATXN2 Gene","authors":"A. A. Dorohova,&nbsp; I. M. Chernukha,&nbsp;L. V. Fedulova,&nbsp;J. L. Hernandez-Caceres,&nbsp;M. I. Drobotenko,&nbsp;S. S. Dzhimak","doi":"10.1134/S1607672925601659","DOIUrl":"10.1134/S1607672925601659","url":null,"abstract":"<p>CAG repeats in the first exon of the <i>ATXN2</i> gene play a key role in the development of a number of neurodegenerative diseases. Understanding the mechanisms underlying CAG tract expansions and the factors influencing them could lead to the development of new methods for the prevention and treatment of these diseases. It was established that a single D/H substitution of a hydrogen bond in the CAG tract increases its stability, thereby reducing the likelihood of formation of secondary structures that interfere with the reading of genetic information from the glutamine-coding region. CAA interruptions in the CAG tract in specific locations can have a similar, but weaker, stabilizing effect.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"45 - 47"},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic Algorithm for Axial Involvement in Psoriatic Arthritis","authors":"E. E. Gubar,&nbsp;T. V. Korotaeva,&nbsp;L. D. Vorobyova,&nbsp;E. Yu. Loginova,&nbsp;Yu. L. Korsakova,&nbsp;P. O. Tremaskina,&nbsp;A. V. Smirnov,&nbsp;M. M. Urumova,&nbsp;S. I. Glukhova","doi":"10.1134/S1607672925700243","DOIUrl":"10.1134/S1607672925700243","url":null,"abstract":"<p>To develop a unified diagnostic algorithm for axial psoriatic arthritis (axPsA).</p><p>A total of 122 patients with psoriatic arthritis (PsA), duration less than 10 years, were included in the study according to CASPAR criteria, provided that they also had axial involvement. Axial involvement was detected in case of radiographic sacroiliitis [(rSI); bilateral grade ≥2 or unilateral grade ≥ 3] or active MRI SI (MRI-SI), or ≥ 1 syndesmophyte(s) of the cervical and/or lumbar spine (CS/LS), or facet joints ankyloses of the CS. Patients were evaluated for the presence of inflammatory back pain (IBP) by ASAS criteria. Back pain lasting over 3 months, that did not meet ASAS criteria was considered chronic back pain (chrBP). HLA-B27 antigen status was observed. </p><p>IBP was identified in 87 (71.3%), chrBP—in 35 (28.7%) patients, 49 (40.2%) patients had older age (&gt;40 years) at back pain onset; 120 (98.4%) patients had peripheral arthritis, 75 (61.5%)—dactylitis, 69 (56.6%)—enthesitis, 122 (100%)—psoriasis, 90 (73.8%)—nail psoriasis. Isolated axial disease without peripheral arthritis was found in 2 (1.6%) patients. rSI was detected in 85 (69.7%) patients, in 28 of 85 (32.9%) patients rSI developed without IBP. Spinal lesions of the LS and CS were found in 100 (82.0%) patients, chunky “non-marginal” syndesmophytes—in 60 (49.2%), asymmetrical syndesmophytes of the LS—in 22 of 72 (30.6%), paravertebral ossification—in 5 (4.1%) patients. Isolated spinal lesions without rSI were found in 37 (30.3%), isolated spinal lesions without rSI or MRI-SI—in 21 (17.2%) patients. HLA-B27 was observed in 27 of 86 (31.4%) examined patients. Diagnostic algorithm for axPsA was developed. All PsA patients, regardless whether they experienced IBP/chrBP or not, must undergo diagnostic imaging: pelvis, LS and CS X-ray. In patients without rSI, MRI of the sacroiliac joints should be performed. AxPsA diagnosis must be confirmed by imaging. Axial involvement is detected in case of rSI or MRI-SI, or ≥1 syndesmophyte(s) of the CS/LS, or facet joints ankyloses of the CS.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"74 - 80"},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Safety of Rapid Dose Escalation of Methotrexate in Rheumatoid Arthritis (Results of the Multicenter METEOR Study)","authors":"V. N. Amirdzhanova,&nbsp;E. Yu. Polischuk,&nbsp;O. N. Anoshenkova,&nbsp;I. B. Vinogradova,&nbsp;E. V. Zonova,&nbsp;L. V. Ivanova,&nbsp;R. R. Samigullina,&nbsp;T. S. Salnikova,&nbsp;E. L. Shakhramanova,&nbsp;E. I. Schmidt,&nbsp;S. P. Yakupova,&nbsp;A. M. Lila,&nbsp; E. L. Nasonov","doi":"10.1134/S160767292570022X","DOIUrl":"10.1134/S160767292570022X","url":null,"abstract":"&lt;p&gt;The aim of the study was to analyze the effectiveness and tolerability of subcutaneous methotrexate (sc MTX) (METHORTRIT; sc ROMPHARM Company) in RA patients with high disease activity with rapid dose escalation, to assess their quality of life (QOL) in real clinical practice.&lt;/p&gt;&lt;p&gt;—The study included 105 patients, mostly women, with a reliable diagnosis of RA with high disease activity (DAS28 ≥ 5.1) aged 18 years and older and ineffectiveness of previous oral MT therapy for at least 6 months or who had not received MT. sc MTX therapy was started at a dose of 15 mg /weekly. During the first month of therapy, a rapid escalation of the scMTX dose of 2.5 mg/week was performed once a week until the dose of 22.5 mg/week was reached; then, with insufficient response, the dose of sc MTX could be increased to 25 mg/week. The evaluation of the effectiveness of therapy, functional status, and QOL was carried out after 4–12–18–24 weeks.&lt;/p&gt;&lt;p&gt;—After a rapid escalation of the sc MTX dose during the first month of the study, at all stages of follow-up, a rapid decrease in disease activity was noted for all standard indices (DAS28 from 5.8 ± 0.75 to 2.93 ± 1.05; CDAI from 30.13 ± 8.33 to 7.08 ± 6.07; SDAI from 32.78 ± 9.64 to 7.48 ± 6.53) and the activity index, which was evaluated by the patients themselves (RAPID-3 from 16.18 ± 4.6 to 5.56 ± 4.66), &lt;i&gt;p&lt;/i&gt; ≤ 0.05. The number of patients with high disease activity according to DAS28 decreased by 2 times by the 4th week of therapy (to 46.2%), after 12 weeks they remained 13.3%, and by 24 weeks high activity remained only in 4.4% of patients. There was a marked decrease in pain from 65.6 ± 13.07 to 20.5 ± 17.1 mm in VAS (&lt;i&gt;p&lt;/i&gt; &lt; 0.001), which contributed to an improvement in the functional state: the HAQ index decreased on average from 1.47 ± 0.65 to 0.64 ± 052 points. Population indices of functional status (HAQ ≤ 0.5) by the 24th week of therapy were observed in 48.9% of patients. A decrease in the level of fatigue (from 6.25 ± 7.04 to 1.81 ± 1.71 cm according to VAS, &lt;i&gt;p&lt;/i&gt; &lt; 0.001) was accompanied by a decrease in anxiety (from 7.47 ± 4.03 to 2.36 ± 2.72, &lt;i&gt;p&lt;/i&gt; &lt; 0.001) and depression (from 7.77 ± 3.84 to 2.50 ± 2.56, &lt;i&gt;p&lt;/i&gt; &lt; 0.001), as well as improved sleep. By the 24th week of the study, 45% of patients had population-based indices of QOL according to the EQ-5D index. Glucocorticoids (GC) were completely eliminated in 2/3 of the patients. Patients who did not receive GC had lower disease activity by 24 weeks in all indices: DAS 28 (2.7 ± 0.1 and 3.4± 0.2, respectively), CDAI (6.0 ± 0.3 and 10.2 ± 0.1), SDAI (6.4 ± 0.2 and 10.9 ± 0.3), &lt;i&gt;p&lt;/i&gt; &lt; 0.05. Patients receiving and not receiving GC had the same number of adverse reactions (&lt;i&gt;p&lt;/i&gt; &gt; 0.05); however, the number of infections in patients receiving GC was significantly higher (9.5%–0.0, respectively, &lt;i&gt;p&lt;/i&gt; = 0.009). The need for nonsteroidal anti-inflammatory drugs (NSAIDs) at the beginning of the study was in 9","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"65 - 73"},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ENY2 Protein Is Present at the Histone Locus Body HLB As Part of Complexes with Different Functions in Transcription and mRNA Export","authors":"S. Y. Ushakova,&nbsp; S. G. Georgieva,&nbsp;M. M. Kurshakova","doi":"10.1134/S160767292560157X","DOIUrl":"10.1134/S160767292560157X","url":null,"abstract":"<p>The evolutionarily conserved transcription factor ENY2 is a component of several distinct complexes that regulate various stages of gene expression: the SAGA transcription coactivator, the THO transcription elongation complex involved in mRNA processing, and the TREX-2 complex responsible for mRNA export from the nucleus to the cytoplasm. Previous works in <i>D. melanogaster</i> have shown that SAGA associates with the histone locus body (HLB) biomolecular condensate, where histone mRNA transcription and processing take place, whereas TREX-2 interacts with histone mRNA and is involved in its export from the nucleus to the cytoplasm. This study demonstrates that ENY2 is a component of the HLB. ENY2 is present at the histone locus on <i>D. melanogaster</i> polytene chromosomes. ENY2 associates with the chromatin of the histone gene cluster as part of the SAGA coactivator complex and the THO transcription elongation complex, which also interacts with histone gene chromatin. ENY2 and subunits of the SAGA, THO, and TREX-2 complexes interact with FLASH, a specific constituent structural component of the HLB. Thus, ENY2 is present at the HLB biomolecular condensate and participates in histone gene expression as part of three functionally diverse complexes: SAGA, THO, and TREX-2.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"40 - 44"},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition and Content of Fatty Acids in Artemia Cyst (Artemia spp.) from Lakes of Southern Siberia as an Indicator of Biochemical Value for Fish Feed","authors":"A. E. Rudchenko,&nbsp;V. A. Karpov,&nbsp;O. S. Dimenko,&nbsp;A. V. Tsyvunina,&nbsp; N. N. Sushchik","doi":"10.1134/S1607672925601830","DOIUrl":"10.1134/S1607672925601830","url":null,"abstract":"<p>For the first time, the composition and fatty acid content of <i>Artemia</i> spp. cysts from three hypersaline lakes in Southern Siberia were analyzed. The fatty acid composition of decapsulated <i>Artemia</i> cysts varied significantly between the studied water bodies. It was found that the cyst profiles from Lakes Cheder and Dus-Khol corresponded to a “marine” type, while those from Lake Tus corresponded to a “freshwater” type. An assessment of the biochemical value, including the content of eicosapentaenoic acid, docosahexaenoic acid, arachidonic acid, the ratio of n-3/n-6 fatty acids, as well as hatching rates (hatching efficiency up to 135 thousand specimens/g, the proportion of hatched nauplii up to 85%), allowed us to recommend cysts from Lake Cheder as a promising source for the production of live feed for aquaculture.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"117 - 121"},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-World Retention Rate, Effectiveness, and Safety of Netakimab in the Treatment of Patients with Ankylosing Spondylitis: Two-Year Results of the Real Word Evidence LIBRA Study","authors":"Sh. F. Erdes,&nbsp;V. I. Mazurov,&nbsp;I. Z. Gaidukova,&nbsp;O. N. Anoshenkova,&nbsp;I. B. Vinogradova,&nbsp;Yu. Yu. Grabovetskaya,&nbsp;S. Yu. Davidian,&nbsp;O. E. Epifanova,&nbsp;N. A. Kiryukhina,&nbsp;L. V. Masneva,&nbsp;I. V. Menshikova,&nbsp;O. N. Mironenko,&nbsp;N. E. Nikulenkova,&nbsp;T. V. Povarova,&nbsp;A. N. Polyatika,&nbsp;R. R. Samigullina,&nbsp;A. E. Sizikov,&nbsp;I. N. Totrov,&nbsp;I. F. Umnova,&nbsp;A. L. Chudinov","doi":"10.1134/S1607672925700279","DOIUrl":"10.1134/S1607672925700279","url":null,"abstract":"<p>—The aim of the study was to obtain data on the safety of netakimab (NTK) in a population of patients with ankylosing spondylitis (AS), including various somatic diseases, as well as to assess treatment retention during 2 years of observation in real world clinical practice.</p><p>—Patients were recruited for the study from August 2020 to December 2021 at 23 centers in the Russian Federation. The study included 137 patients who were prescribed netakimab therapy before enrollment. Clinical and medical history data for the first visit were entered retrospectively, and following visits at 12, 24, 52, 76, and 104 weeks of therapy were collected within the study. The average age of the patients 42.3 years, 34.3% of them with previous biologics therapy.</p><p>—Median observation period was 104 weeks (range 1–137 weeks). At the end of the analyzed period (104 weeks of therapy), 85.5% (95% confidence interval (95% CI): 79.7–91.8) of patients continued treatment with netakimab. Retention on NTK therapy was slightly better in “bio-naïve” vs patients who received biologics earlier: 88.7% (95% CI: 82.3–95.5) and 78.9% (95% CI: 67.5–92.2), respectively, without significant differences between groups (<i>p</i> = 0.16). As many as 21 (15.3%) patients withdrew from study before visit 6. The main end-of-study reasons were lost to follow-up (7 (5.1%) patients) and treatment inefficacy (6 (4.4%) patients). The BASDAI (Bath Ankylosing Spondylitis Disease Activity Index) and ASDAS-CRP (Ankylosing Spondylitis Disease Activity Score with C-reactive protein) showed statistically significant decreases from baseline: by 3 times during the first 3 months of therapy and 2 times decrease during the first year of treatment. This trend continued in the second year of treatment, although with a lower rate of reduction. By week 104 of therapy, 52.9% (95% CI: 47.3–58.4) reached low disease activity (1.3 ≤ ASDAS &lt; 2.1), 21.3% (95% CI: 12.8–29.8) had inactive disease (ASDAS &lt; 1.3). Netakimab was well tolerated by patients: AEs, related to therapy according to the investigator’s opinion, were reported in 8 (6.0%) patients.</p><p>—In real-world clinical practice, 85.5% of patients continued treatment with Netakimab at the end of 104 weeks. By 104 weeks 74% patients had low disease activity or inactive disease. Netakimab was well tolerated by most of patients.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"122 - 131"},"PeriodicalIF":0.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147580126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathomorphological Features of Lung Damage Caused by Infection with Various Variants of SARS-CoV-2 in Humanized Model Animals","authors":"A. S. Chernov,&nbsp;V. A. Kazakov,&nbsp;I. S. Gogleva,&nbsp;F. A. Mescheryakov,&nbsp;A. A. Kudriaeva,&nbsp;A. P. Bogachuk,&nbsp; I. V. Smirnov,&nbsp;G. B. Telegin,&nbsp;A. A. Belogurov,&nbsp; A. G. Gabibov","doi":"10.1134/S1607672925601787","DOIUrl":"10.1134/S1607672925601787","url":null,"abstract":"<p>The genotypic variability of the SARS-CoV-2 virus is extremely high, and the emergence of new strains raises concerns about their possible high virulence and ability to bypass responses of the body’s immune system induced by previous infection or vaccination. Therefore, one of the main tasks is to study the pathogenesis of various variants of the virus using experimental animal biomodels of SARS-CoV-2 to quickly find methods and approaches to fighting new viruses. The study was performed on humanized mice of the C57BL/6-Tgtn line. Mice were infected intranasally at different doses with three variants of the SARS-CoV-2 virus. We showed that humanized hACE2 mice, when infected with all three variants of the SARS-CoV-2 virus, showed typical pathological changes in lung consistency comparable to those found in COVID-19 in humans. At a dose of 4 log plaque-forming unit (PFU), all variants showed 100% mortality. In a comparative assessment of different variants of the SARS-CoV-2 virus in hACE2 humanized mouse model, it was found that the Delta variant leads to more severe damage compared to Wuhan or Omicron.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"17 - 24"},"PeriodicalIF":0.7,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1607672925601787.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of Biochemical and Hematological Parameters and Chromosomal Disorders in Lymphocytes of Aging Primates of the Kurchatovsky Complex of Medical Primatology","authors":"O. P. Chzhu,&nbsp;N. S. Rudenko,&nbsp;D. E. Araviaashvili,&nbsp;R. V. Panfilov,&nbsp;D. A. Dushin,&nbsp;I. I. Marinich,&nbsp;A. V. Popov","doi":"10.1134/S1607672925601751","DOIUrl":"10.1134/S1607672925601751","url":null,"abstract":"<p>Aging is a key challenge for modern society. In particular, brain aging is accompanied by chronic inflammation, depletion of energy potential, and an increased level of oxidative stress, with changes in blood composition playing a special role in this process. Recent studies also show that aging progresses non-linearly throughout life. Primates are genetically and anthropometrically the closest laboratory animals to humans, thus representing the most accurate model for research. This study establishes baseline values for biochemical parameters (including the state of the body’s antioxidant system), cellular-hematological, and genomic indicators in aging primates of various species, sexes, and ages from the Kurchatov Complex of Medical Primatology. In aging males, the concentration of lipid peroxidation products was lower than in females of the same age and species. Analysis of antioxidant defense parameters indicates a more stable redox balance in old cynomolgus macaques of both sexes, which may be associated with their lower aggressiveness and high adaptability. The biochemical profile analysis in aging rhesus macaques revealed that females exhibit elevated levels of all measured parameters. In aging cynomolgus macaques, there are fewer sex-related differences in blood composition characteristics compared to rhesus macaques. It can be noted that under the housing conditions of the primates at the nursery of the Kurchatov Complex of Medical Primatology, several types of aging based on blood parameters can be observed within the same age category across different species and sexes.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"1 - 10"},"PeriodicalIF":0.7,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1607672925601751.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Antioxidant Defense Genes Determines Synergistic Ferroptosis Induction by the Combination of Erastin and Omega-3 Docosahexaenoic Acid in Prostate Cancer Cells","authors":"M. O. Silkina,&nbsp;T. A. Kulagin,&nbsp;K. V. Klycheva,&nbsp;A. D. Shatsillo,&nbsp;M. D. Mastykina,&nbsp;A. V. Razumovskaya,&nbsp;K. M. Nyushko,&nbsp;S. V. Nikulin","doi":"10.1134/S1607672925601660","DOIUrl":"10.1134/S1607672925601660","url":null,"abstract":"<p>Ferroptosis is considered a promising strategy for inducing the death of tumor cells. However, the effectiveness of known ferroptosis inducers, such as erastin, is in some cases limited, which stimulates the search for new combined application strategies. In this study, the combined effect of erastin and docosahexaenoic acid (DHA) on prostate cancer cells was examined over time. It was shown that the combination of these agents is more toxic compared to their separate use for all tumor cells considered. At the same time, known ferroptosis inhibitors, ferrostatin-1 and deferoxamine, effectively prevented cell death, indicating the specificity of the mechanism of action. Transcriptomic analysis of cell lines differing in sensitivity to the combination revealed activation of antioxidant systems in more resistant cells (in particular, pronounced expression of the <i>NQO1</i> and <i>GCLM</i> genes responsible for the reduction of quinones to hydroquinones and the synthesis of glutathione, respectively). The obtained results indicate the high synergistic potential of the erastin–DHA combination for ferroptosis induction and open new possibilities for the development of combined approaches to the therapy of resistant tumors.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"526 1","pages":"11 - 16"},"PeriodicalIF":0.7,"publicationDate":"2026-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1607672925601660.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146117363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Textural Properties of Pectin–Carrageenan Hydrogels Produced by Food 3D Printing","authors":"S. V. Popov,&nbsp;F. V. Vityazev,&nbsp;N. M. Paderin,&nbsp;V. V. Smirnov,&nbsp;D. S. Khramova,&nbsp;L. A. Bakutova","doi":"10.1134/S1607672925601489","DOIUrl":"10.1134/S1607672925601489","url":null,"abstract":"<p>This study investigated the printability and textural properties of 3D-printed pectin–κ-carrageenan hydrogels with varying polysaccharide ratios. Inks with 50 and 60% κ-carrageenan exhibited optimal printability, producing structures consistent with digital designs. Rheological temperature sweep tests showed increased viscosity and moduli with higher κ-carrageenan content, affecting extrusion behavior. Hydrogels printed with high-methoxyl (HM) pectin were softer, less cohesive, and more adhesive than those with low-methoxyl (LM) pectin, attributable to lower network strength confirmed by rheological tests. Sensory evaluations favored HM hydrogels for ease of swallowing and taste, supported by reduced chewing effort and enhanced saliva absorption. These findings demonstrate that κ-carrageenan improves pectin ink printability and enables fabrication of edible gels with tunable texture for personalized nutrition.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 2","pages":"670 - 676"},"PeriodicalIF":0.7,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146083142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}