Doklady Biochemistry and Biophysics最新文献

{"title":"Genomic Diversity of Toxigenic Strains of Vibrio cholerae O1 Biovar El Tor Isolated During Three Waves of the 7th Cholera Pandemic","authors":"N. I. Smirnova,&nbsp;D. A. Rybalchenko,&nbsp;Yu. V. Lozovsky,&nbsp;Ya. M. Krasnov,&nbsp;E. Yu. Shchelkanova,&nbsp;A. V. Fedorov,&nbsp;V. V. Kutyrev","doi":"10.1134/S1607672925700218","DOIUrl":"10.1134/S1607672925700218","url":null,"abstract":"<p>High genome variability of the 7th cholera pandemic agent, <i>V. cholerae</i> El Tor, led to emergence of genovariants with a distinct set of altered genes. The aim of the work was to analyze the dynamics of changes in pathogenicity, epidemicity, as well as drug resistance and phylogeny in toxigenic strains of <i>V. cholerae</i> El Tor isolated in Russia and endemic regions during three waves of ongoing pandemic. <i>Materials and methods.</i> We used whole-genome nucleotide sequences of 155 strains, obtained by us (42) and taken from the NCBI Genbank (113). DNA sequencing was performed on Ion PGM platform. Phylogenetic relations were determined based on the Bayesian analysis of core SNPs obtained using Snippy 4.6 software package. Antibiotic resistance was assessed using the disk diffusion test. <i>Results.</i> SNP data revealed that the studied strains (1970–2023) can be divided into three clusters. A clear correlation between each-cluster strain genotype and relevant isolation timing was observed. Separation of genetically altered cluster II and III strains isolated during the 2nd and 3rd waves of the pandemic from typical cluster I strains is associated with the acquisition of new DNA regions and mutations in pathogenicity and drug resistance genes. Due to different combination of mutations, cluster III strains are genetically heterogeneous. Genome comparison showed that this diversity increased dramatically during the 3rd wave, which led to emergence of new genovariants with higher pathogenic and epidemic potential. It is demonstrated that antibiotic resistance in strains both from endemic regions and Russia over the past 30 years (1993–2023) has undergone significant changes. The changing drug resistance clearly correlated with the occurrence of mutations in various pathogenicity genes. <i>Conclusions.</i> It is shown that, over the past two decades, the agent genome underwent a rather rapid change resulting in emergence of various genovariants. A change in the pathogen variants in Russia has been established. Strains combining genetic markers of hyper-virulence and multiple drug resistance are of particular concern. Genome variability of the strains identified points at a need for constant genomic surveillance to obtain data on altering epidemically important properties for timely generation of new diagnostic and preventive means.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"593 - 604"},"PeriodicalIF":0.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and Pharmacokinetics of Nanosized NH2‑UiO‑66 (Zr) Metal-Organic Frameworks","authors":"A. B. Mirkasymov,&nbsp;V. I. Rodionov,&nbsp;D. A. Pokhorukov,&nbsp;O. Yu. Griaznova,&nbsp;N. K. Ivshina,&nbsp;I. V. Lunyov,&nbsp;I. V. Zelepukin,&nbsp; S. M. Deyev","doi":"10.1134/S1607672925601477","DOIUrl":"10.1134/S1607672925601477","url":null,"abstract":"<p>The application of porous nanomaterials in drug delivery offers a promising strategy to mitigate the adverse side effects of chemotherapy. In this study, we report the synthesis of nanosized NH₂-UiO-66 (Zr) metal-organic frameworks as carriers of doxorubicin. The nanoparticles exhibited high crystallinity with an average size of 44 nm. Surface functionalization with polyethylene glycol (PEG) markedly enhanced their colloidal stability under physiological conditions. Coated NH₂-UiO-66 (Zr)@PEG particles demonstrated prolonged circulation in the bloodstream and a significant reduction of nonspecific accumulation in organs with high vascularization. Importantly, these particles retained their capacity for doxorubicin loading, highlighting their potential for drug delivery.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"569 - 573"},"PeriodicalIF":0.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1607672925601477.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salicylate-Induced Changes of Protein Secretion into Apoplast in Pea Roots Is Not Always Associated with Increased Expression of Genes Encoding Them","authors":"A. M. Egorova","doi":"10.1134/S1607672925601210","DOIUrl":"10.1134/S1607672925601210","url":null,"abstract":"<p>This work presents the results of the analysis of the effect of the phytohormone salicylic acid on the secretion of proteins to the apoplast of the pea seedlings roots. It is shown that the secretion of a number of defense proteins (β-1,3-glucanase, acid and alkaline endochitinases, disease resistance response protein, and protease inhibitors) is increased, but there is no activation of the expression of the genes encoding these proteins. A distinctive feature of the pea root response to the salicylic acid was the activation of the expression of the genes encoding isoforms of chitinase-like proteins and the secretion of these proteins into the apoplast.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"562 - 568"},"PeriodicalIF":0.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SAGA Transcription Coactivator Complex Is Associated with Histone Locus Body HLB","authors":"Y. A. Yakusheva,&nbsp; S. G. Georgieva,&nbsp;M. M. Kurshakova","doi":"10.1134/S1607672925601520","DOIUrl":"10.1134/S1607672925601520","url":null,"abstract":"<p>The chromatin of histone genes is associated with specific nuclear biomolecular condensate called the histone locus body (HLB). HLB is the compartment where the transcription of histone genes and processing of their pre-mRNAs coordinated with the cell cycle take place. In this work, the transcriptional histone acetyltransferase coactivator complex SAGA is described as a component of the HLB. Specific subunits of the SAGA complex, Gcn5 and Sgf11 proteins, are present on the chromatin locus of the histone gene cluster and interact with known constant specific structural components of the HLB (Mxc, FLASH, and Mute proteins).</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"580 - 584"},"PeriodicalIF":0.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-Dose MLN4924 Enhances SH-SY5Y Cell Viability and Migration by Targeting SOCS3 Signaling","authors":"Zelin Lai,&nbsp;Simin Yang,&nbsp;Xia Liu,&nbsp;Zhili Liu","doi":"10.1134/S160767292560160X","DOIUrl":"10.1134/S160767292560160X","url":null,"abstract":"<p>MLN4924 (pevonedistat), a selective inhibitor of the NEDD8-activating enzyme, has inhibitory effects on various tumors by blockade of the neddylation pathway. Conversely, recent studies show that low-dose MLN4924 exerts pro-survival effects in both cancer cells and neurons, however, its actions on SH-SY5Y cells remain unclear. In this study, the effects of low-dose MLN4924 in SH-SY5Y cells were assessed by CCK-8 and Transwell assay, respectively. SH-SY5Y cells were transfected with enhanced green fluorescent protein plasmid to further observe neurite changes. The expression of SOCS3, p-JAK2 and p-STAT3 were analyzed by Western blot, and the potential interaction between MLN4924 and SOCS3 was explored via in silico molecular docking. Our results showed that 0.1 μM MLN4924 significantly enhanced SH-SY5Y cell viability and migration while concurrently reducing neurite outgrowth. The expression level of SOCS3 was significantly increased in the MLN4924 treatment group compared with the control group, but the phosphorylation levels of JAK2 and STAT3 showed no changes. Molecular docking further predicted that Glu63 of SOCS3 serves as a key residue for MLN4924 binding. Together, low-dose MLN4924 enhances SH-SY5Y cell viability and migration by targeting SOCS3 signaling, independent of JAK2/STAT3 signaling.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"585 - 592"},"PeriodicalIF":0.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Interactions between three Su(Hw)-Dependent Insulators and the White Gene Enhancer on Long-Distance Transcriptional Regulation in Drosophila melanogaster","authors":"L. S. Melnikova,&nbsp; P. G. Georgiev,&nbsp;A. K. Golovnin","doi":"10.1134/S1607672925601593","DOIUrl":"10.1134/S1607672925601593","url":null,"abstract":"<p>Special regulatory elements, insulators, either block enhancer-promoter contacts or facilitate long-distance interactions between regulatory elements of the genome. Two identical DNA sequences containing four Su(Hw) protein binding sites were shown to interact in a transgenic system and ensure maximal activation of the <i>yellow</i> and <i>white</i> promoters by specific enhancers located 28 kb apart. We demonstrated that three Su(Hw) insulators are also capable of regulating specific enhancer–promoter contacts at a distance of 28 kb. The effect of multiple insulators on transcription depends on the relative positions of insulators, enhancers, and promoters. We confirmed that the eye enhancer directly interacts with the Su(Hw) insulator and participates in the formation of chromatin loops. These data indicate that insulators are multifunctional elements that organize chromatin structure and directly regulate gene activity.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"574 - 579"},"PeriodicalIF":0.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145950658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ELOVL5 Regulates Ferroptosis in Breast Cancer Cells","authors":"K. V. Klycheva,&nbsp;A. V. Razumovskaya,&nbsp;A. D. Shatsillo,&nbsp;M. D. Mastykina,&nbsp;T. A. Kulagin,&nbsp;M. O. Silkina,&nbsp;S. V. Nikulin","doi":"10.1134/S1607672925601246","DOIUrl":"10.1134/S1607672925601246","url":null,"abstract":"<p>Today, breast cancer (BC) occupies a leading position in prevalence and mortality from oncological diseases among the female population worldwide. Ferroptosis is a special type of cell death associated with peroxidation of intracellular lipids. It is a promising option for the therapy of BC resistant to traditional methods of treatment. The <i>ELOVL</i>5 gene, involved in the elongation of long-chain polyunsaturated fatty acids (LC-PUFA), was previously associated with BC progression. In this work, the effect of <i>ELOVL</i>5 knockdown on the dynamics of ferroptosis induction in MDA-MB-231 cells under the influence of docosahexaenoic acid (DHA) and erastin was investigated. A comparative analysis of changes in the expression of individual genes under the influence of these agents was also carried out. It was shown that a decrease in <i>ELOVL</i>5 expression increases cell sensitivity to both agents, while DHA causes earlier cell death. The protective effect of ferroptosis inhibitors (ferrostatin-1 and deferoxamine) confirmed the involvement of this pathway in the observed effects. Differences in the expression of genes associated with oxidative stress, inflammation and proliferation were also revealed, indicating different molecular trajectories of ferroptosis in cells with different <i>ELOVL</i>5 gene expression. Thus, the present study deepens the understanding of the contribution of the <i>ELOVL</i>5 gene to the regulation of ferroptosis and can be used in the development of targeted therapy for breast cancer.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"532 - 540"},"PeriodicalIF":0.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1607672925601246.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Temperature on Salicylate-Induced Components in Tomato Plants Immune System under Invasion with Root-Knot Nematode Meloidogyne incognita","authors":"S. V. Zinovieva,&nbsp;Zh. V. Udalova,&nbsp;F. K. Khasanov,&nbsp; M. S. Gins","doi":"10.1134/S1607672925601386","DOIUrl":"10.1134/S1607672925601386","url":null,"abstract":"<p>The tomato gene <i>Mi-1,2</i> is currently the only commercially available source of resistance to the root-knot nematode <i>Meloidogyne incognita</i>, which loses activity when soil temperatures exceed 28°C. The study aimed to investigate the components of the tomato immune system to root-knot nematode associated with salicylic acid (SA) at elevated (34°C) and normal (25°C) temperatures. The obtained results showed that the <i>Mi-1,2</i>-mediated immune response is disrupted at 34°C. At elevated temperatures in plants infested with nematodes, the synthesis of salicylic acid and the activity of phenylalanine ammonia lyase are reduced, and catalase is inhibited, a decrease in the activity of which was discovered at the stage of nematode penetration into the roots and the creation of feeding structures—giant cells. Elevated temperature decreased the activity of the <i>PR-1</i> gene, a marker of systemic plant resistance. These results provide important information on the sensitivity temperature of the <i>Mi-1.2</i> resistance gene and the effect of elevated temperature on SA-dependent components of the immune system that are associated with tomato resistance to the nematode.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"553 - 557"},"PeriodicalIF":0.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"20-Hydroxyecdysone Modulates Gene-Specific Immune Response to Metarhizium anisopliae Infection in Drosophila melanogaster S2 Cell Culture","authors":"M. Ghassah,&nbsp;Y. A. Ulianova,&nbsp; P. G. Georgiev,&nbsp;Y. V. Shidlovskii,&nbsp;Z. M. Kachaev","doi":"10.1134/S1607672925601398","DOIUrl":"10.1134/S1607672925601398","url":null,"abstract":"<p>The interplay between innate immunity and other signaling pathways remains a central focus in immunological research, with considerable ongoing investigation. Of particular interest are studies exploring the influence of the hormonal system on the innate immunity of <i>Drosophila melanogaster</i>. In this study, we performed a comprehensive analysis of the combined effects of the insect hormone 20-hydroxyecdysone (20E) and spores from the fungus <i>Metarhizium anisopliae</i> on the innate immune response in <i>D. melanogaster</i> S2 cell culture, addressing this interaction for the first time. Our results demonstrate that, compared to cells exposed solely to <i>M. anisopliae</i> spores, pretreatment with 20E followed by fungal challenge led to a reduction in the transcription of antimicrobial peptide genes <i>CecropinA1</i> and <i>Drosocin</i>. In contrast, expression of the <i>Metchnikowin</i> (<i>Mtk</i>) gene was upregulated. No significant alterations were observed in the transcription levels of <i>Drosomycin</i> or in genes encoding key receptors, transcription factors, or other components of innate immune signaling pathways. Furthermore, knockdown of the transcription factor Relish markedly decreased <i>Mtk</i> expression, highlighting its central role in hormone-modulated antifungal immunity. These findings reveal complex hormonal–immune crosstalk that differentially regulates AMP gene expression in <i>Drosophila</i>.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"558 - 561"},"PeriodicalIF":0.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MLE/DHX9 without Helicase Activity Activates Constitutive Expression of Nuclear Receptor Genes in Drosophila melanogaster","authors":"I. A. Zolin,&nbsp;A. A. Grigel,&nbsp; S. G. Georgieva,&nbsp;J. V. Nikolenko","doi":"10.1134/S1607672925601180","DOIUrl":"10.1134/S1607672925601180","url":null,"abstract":"<p>MLE/DHX9 is a DNA/RNA helicase that performs important functions in gene expression regulation in eukaryotes. However, the specific role of MLE and the mechanisms by which this regulation is carried out remain poorly understood. This work is devoted to the study of the effect of MLE on the constitutive expression of nuclear receptor genes in <i>D. melanogaster</i>. The nuclear receptors Eip75B, DHR3, and Hr4 are evolutionarily conserved and have orthologs in humans. In <i>D. melanogaster</i>, <i>Eip</i>75<i>B, DHR</i>3, and <i>Hr</i>4 are activated in the ecdysone cascade, but they are also expressed at a stable level in various tissues and at various stages of development. The experiments were carried in vivo at the imago stage in females and in S2 cell culture. Taken together, the results indicate that MLE is involved in activating the expression of these genes. At the same time, the helicase activity of MLE is not necessary for activation. The results obtained extend knowledge about the functions of MLE beyond dosage compensation, as potentially conserved in evolution, and contribute to understanding the mechanisms of regulation of nuclear receptor expression.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"527 - 531"},"PeriodicalIF":0.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}