Doklady Biochemistry and Biophysics最新文献

{"title":"Anti-Carbamylated Protein Antibodies in ACPA-Negative and ACPA-Positive Patients with Rheumatoid Arthritis","authors":"D. A. Dibrov,&nbsp;A. S. Avdeeva,&nbsp;M. E. Diatroptov,&nbsp; E. L. Nasonov","doi":"10.1134/S1607672924700960","DOIUrl":"10.1134/S1607672924700960","url":null,"abstract":"<p>The objective of this study was to assess the level of antibodies to carbamylated proteins and analyze the clinical and immunological associations in patients with ACPA-negative and ACPA-positive variants of rheumatoid arthritis.</p><p>. The study involved 150 patients with a reliable diagnosis of rheumatoid arthritis and 25 patients as healthy controls. Depending on ACPA values, two groups of patients were recruited: ACPA-positive (<i>n</i> = 75) and ACPA-negative (<i>n</i> = 75). RA activity was assessed by the DAS28 index. Determination of antibodies to carbamylated proteins was performed by enzyme-linked immunosorbent assay (BlueGene Biotech, China). Quantitative determination of ACPA in serum was performed by enzyme immunoassay using a commercial reagent kit (AxisShield, UK; upper limit of normal 5.0 U/mL; Orgentec, Germany; upper limit of normal 20.0 U/mL).</p><p>. Median anti-CarP in patients with RA was 126.2 [100.83; 157.41] ng/mL and was statistically significantly higher (<i>p</i> &lt; 0.001) than in healthy controls (88.89 [70.53; 107.75] ng/mL). Among all patients with RA, 50 (33.3%) were anti-Carp-positive (22 (29.3%) in the ACPA(+) group and 28 (37.3%) in the ACPA(–) group), and one (2%) volunteer from healthy controls was anti-CarP(+) (<i>p</i> = 0.002). In ROC analysis performed to assess the diagnostic significance of anti-CarP for RA for all patients with RA, the area under the curve was 0.783 ± 0.047 with 95% CI: 0.691–0.874 (<i>p</i> &lt; 0.001), with a cut-off point of 143 ng/mL, specificity 96%, sensitivity 36.7%.</p><p>In the ACPA(+) RA group, the erosion count was statistically significantly higher (<i>p</i> = 0.044) in anti-CarP(+) patients than in anti-CarP(–) patients. A weak direct correlation between anti-CarP and DAS28 was found in the ACPA(–) RA group.</p><p>. We studied the predictive value of anti-CarP as an auxiliary biomarker in ACPA(+) and ACPA(–) subtypes of RA. ACPA(+) anti-CarP(+) patients have a more “erosive” subtype of the disease than ACPA(+) anti-CarP(–) patients. In ACPA(–) patients, anti-CarP helps to identify a more erosive subtype of the disease, and among ACPA(–) patients it helps to reduce the proportion of seronegative patients. Further studies are required to determine the optimal standards for the laboratory diagnosis of anti-CarP and to clarify the diagnostic potential of these ABs as part of the differential diagnosis of arthritis in other rheumatic diseases.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"517 1","pages":"235 - 242"},"PeriodicalIF":0.8,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Features of ACPA-Negative and ACPA-Positive Variants of Rheumatoid Arthritis","authors":"D. A. Dibrov,&nbsp;A. S. Avdeeva,&nbsp;V. V. Rybakova,&nbsp;N. V. Demidova,&nbsp; E. L. Nasonov","doi":"10.1134/S1607672924700996","DOIUrl":"10.1134/S1607672924700996","url":null,"abstract":"<p>The aim of the study was to investigate the features of the clinical picture of the disease in patients with ACPA–negative and ACPA-positive variants of rheumatoid arthritis.</p><p>The study included patients with a reliable diagnosis of rheumatoid arthritis (RA) according to the criteria of ACR/EULAR 2010. Depending on the ACPA values, two groups of patients were recruited: ACPA-positive and ACPA-negative, comparable in gender, age, duration of the disease, and therapy. The nature of the onset and course of the disease and the activity of RA were evaluated (according to the DAS28, SDAI, CDAI indices).</p><p>The study involved 79 patients with ACPA-negative variant of RA and 79 ACPA-positive patients. The age of patients (Me [IR] (in years)) with the ACPA(–) variant was 52 [39; 62]; with the ACPA(+) variant, 54 [42; 62]; the duration of the disease (in months) was 59 [23; 122] and 48 [17; 84], respectively.</p><p>In ACPA(+) patients, a higher disease activity was determined (by the indices DAS 28crp, DAS28esr, SDAI, CDAI), higher values of C-reactive protein and erythrocyte sedimentation rate, and a greater number of painful and swollen joints (<i>p</i> &lt; 0.05).</p><p>According to the localization of the involved joints, arthritis of the proximal interphalangeal, metacarpal, wrist and shoulder joints was more often determined in ACPA(+) patients.</p><p>Systemic manifestations of RA at the time of examination and in the anamnesis were statistically significantly more common in ACPA(+) (32.9%) than in ACPA(–) (17.7%) patients. Of the systemic manifestations, rheumatoid nodules were more common in ACPA(+) patients, whereas a tendency to a higher frequency of neuropathy, sclerites, and episcleritis was revealed in ACPA(–) patients.</p><p><b>.</b> In patients with ACPA(–) subtype, clinical signs of joint damage and the inflammatory component are less pronounced compared to ACPA(+). However, the mixed picture of manifestation, the less “bright” course of the disease, the absence of characteristic immunological biomarkers necessitate long-term and careful monitoring of this group of patients. At the same time, the subjective severity of the disease and dysfunction due to ankylosing joints do not differ from the ACPA(+) variant of RA.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"517 1","pages":"243 - 249"},"PeriodicalIF":0.8,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition and Content of Fatty Acids in Muscle Tissue of the Potanin Altai Osman Oreoleuciscus potanini (Cypriniformes, Actinopterigii) from Mongolian Reservoirs","authors":"Yu. Yu. Dgebuadze,&nbsp; N. N. Sushchik,&nbsp;B. Mendsaikhan,&nbsp;D. Altansukh,&nbsp;A. Y. Emelianova,&nbsp; M. I. Gladyshev","doi":"10.1134/S160767292470100X","DOIUrl":"10.1134/S160767292470100X","url":null,"abstract":"<p>The composition of fatty acids in the muscle tissue of the unique Central Asian carp-like fish, Potanin Altai osman <i>Oreoleuciscus potanini</i>, was studied for the first time. The populations of these fish in the reservoirs of the semiarid zone (Durgun and Taishir) during the period of their formation are considered. It was shown that the content of eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids in <i>O. potanini</i> corresponds to the median of this value in the order Cypriniformes. It was established that the basis of the food web of the herbivorous form of this species consists of microalgae (diatoms, <i>Euglena</i> and, possibly, chrysophytes), as well as bacteria. At the same time, the levels of bacterial biomarkers, 15-17BCFA and 17:0 were significantly higher in fish in the Durgun reservoir, whereas the level of EPA (diatom biomarker) in <i>O. potanini</i> was higher in the Taishir reservoir. The established higher values of the heavy nitrogen isotope content in the muscles of <i>O. potanini</i> from the Taishir reservoir are most likely associated with the yet unformed benthic communities and with the incomplete diversification of the riverine form of the Potanin Altai osman into lacustrine forms.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"300 - 304"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of Life Cycles of Nematodes Parasitizing Woody Plants As a Result of Ecological and Phylogenetic Co-Adaptations with Hosts and Vectors","authors":"A. Yu. Ryss","doi":"10.1134/S1607672924701047","DOIUrl":"10.1134/S1607672924701047","url":null,"abstract":"<p>Fundamental aspects in the evolution of nematodes parasitizing woody plants are reviewed. (1) Nematode faunal lists of natural refugia are useful to predict the risks of opportunistic pathogens becoming true pathogens in the forest and park communities. (2) Nematode composition in natural refugia gives a chance to identify nematode antagonists of insect vectors of dangerous fungal and nematode infections, which can be potentially used as the biological agents for woody plants’ protection. (3) Dauers in the ancestors of wood-inhabiting nematodes played a role as a survival stage in the detritus decomposition succession, and they later acquired the functions of dispersal and adaptations for transmission using insect vectors. (4) When inspecting wilted trees, it is necessary to use dauers for diagnostics, as sexually mature nematodes may be absent in tree tissues. (5) Plant parasitic nematodes originated from members of the detritus food web and retained a detritivorous phase in the life cycle as a part of the propagative generation. (6) Vectors in the life cycles of plant parasitic nematodes are inherited from the ancestral detritivorous nematode associations, rather than inserted in the dixenic life cycle of the ‘nematode–fungus–plant’ association. (7) Despite the significant difference in the duration of the nematode–tree and nematode–vector phases of the life cycle, the actual parasitic nematode specificity is dual: firstly to the vector and secondly to the natural host plant (as demonstrated in phytotests excluding a vector).</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"325 - 345"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reconstruction of Derogenes varicus Miracidium (Digenea: Derogenidae): First Ultrastructural Description of Spines on the Surface of Hemiurata Larvae","authors":"P. A. Smirnov,&nbsp;D. Yu. Krupenko","doi":"10.1134/S1607672924701059","DOIUrl":"10.1134/S1607672924701059","url":null,"abstract":"<p>We performed a detailed ultrastructural reconstruction of the “passive” miracidium of <i>Derogenes varicus</i> Muller, 1784 , a species from the Hemiurata group. The miracidium is highly miniaturized and simplified in comparison with the “active” miracidia. For the first time we elucidated the nature of the spines on the surface of the hemiuroid larva: they are derivatives of the epithelial plates. The anterior end of the larva is equipped with three epithelial plates that bear both spines and cilia. The major part of the miracidial surface is formed by the tegument. The nervous and excretory systems of the <i>D. varicus</i> miracidium are extremely reduced. Single undifferentiated cell comprises the germinal material of the miracidium. We discuss the trends of evolution of hemiuroid miracidia that are associated with the transition to passive strategy of infection.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"346 - 354"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Results of a 12-Week Open-Label, Non-Interventional Study of the Efficacy and Safety of Olokizumab Therapy in Patients with Rheumatoid Arthritis after Switching from Anti-B-Cell Therapy during the SARS-CoV-2 Pandemic","authors":"A. A. Akimova,&nbsp;N. E. Banshchikova,&nbsp;A. E. Sizikov,&nbsp;A. A. Mullagaliev,&nbsp;E. A. Letyagina,&nbsp;N. A. Ilina,&nbsp;Y. D. Kurochkina,&nbsp;Y. B. Ubshaeva,&nbsp;V. O. Omelchenko,&nbsp;O. A. Chumasova,&nbsp;N. S. Shkaruba,&nbsp;M. A. Korolev","doi":"10.1134/S1607672924701060","DOIUrl":"10.1134/S1607672924701060","url":null,"abstract":"&lt;p&gt;The COVID-19 pandemic has significantly changed the understanding of the safety profile of therapies for immunoinflammatory rheumatic diseases (IRDs). This is primarily due to the negative impact of a number of basic disease-modifying antirheumatic drugs (DMARDs) and genetically engineered biological drugs (biological DMARDs, or biologics) on the course and outcomes of a new coronavirus infection. A number of studies have shown that anti-B-cell therapy (rituximab) gave a statistically significant increase in the risk of severe COVID-19 and an increase in mortality. At the same time, the analysis of real clinical practice data dictated the need to establish a number of restrictions on the use of certain classes of biologics and to search for alternative therapy programs to maintain control over disease activity.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Purpose of the study.&lt;/b&gt; The purpose of the study was to evaluate the efficacy and safety of the drug Artlegia® (olokizumab), solution for subcutaneous injection, 160 mg/ml-0.4 ml, manufactured by R-Pharm JSC, Russia) for the treatment of patients with rheumatoid arthritis in real clinical practice after switching with rituximab during the COVID-19 pandemic.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Materials and methods.&lt;/b&gt; The study included 14 patients with a confirmed diagnosis of rheumatoid arthritis (RA), who were previously on rituximab therapy at a dose of 1000–500 mg twice with an interval of 2 weeks, who received at least one course of therapy with this drug. As RA worsened, patients were switched to olokizumab against the background of standard DMARDs. On weeks 0, 4, 8, and 12 after the switch, the severity of pain was assessed on the VAS scale, the number of tender and swollen joints (TJC28 and SJC28), the level of acute-phase inflammation markers, the DAS28 (disease activity score), ESR, CRP, CDAI (clinical activity index), and the functional state index HAQ (Health Assessment Questionnaire) were determined, as well as the safety profile of therapy was assessed.&lt;/p&gt;&lt;p&gt;&lt;b&gt;Results.&lt;/b&gt; Data analysis was performed using median values (Me) were used for data analysis. A significant decrease in TJC28 was detected after 8 and 12 weeks of treatment with olokizumab (Artlegia®) (Me baseline = 10, Me 8 weeks = 4, Me 12 weeks = 4, &lt;i&gt;p&lt;/i&gt; &lt; 0.05) and a decrease in TSC28 was detected after 4, 8, and 12 weeks of treatment (Me baseline = 9, Me 4 weeks = 3.5, Me 8 weeks = 2.5, Me 12 weeks = 2.0, &lt;i&gt;p&lt;/i&gt; &lt; 0.05). Laboratory markers of inflammation showed a decrease in CRP and ESR levels after 4 weeks of treatment (CRP: Me4 weeks = 21, Me4 weeks = 1, &lt;i&gt;p&lt;/i&gt; &lt; 0.05, ESR: Mesno = 31, Me4 weeks = 7, &lt;i&gt;p&lt;/i&gt; &lt; 0.05). Positive dynamics persisted on 8 and 12 weeks (CRP: Me 8 weeks = 1, Me 12 weeks = 0; ESR: Me 8 weeks = 4, Me 12 weeks = 5). The level of CRP by week 4 became within the normal range, regardless of the initial values. All activity indices improved from week 4 in each evaluation period compared to baseline: DAS28-ESR: Me baseline = 5.52, ","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"291 - 299"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organization of the Nuclei in the Tegument of Some Palaeacanthocephala and Archiacanthocephala","authors":"V. P. Nikishin,&nbsp;D. V. Ponomarev","doi":"10.1134/S1607672924701023","DOIUrl":"10.1134/S1607672924701023","url":null,"abstract":"<p>The giant tegument nuclei of the acanthocephalans of the classes Archiacanthocephala and Palaeacanthocephala are fragmented at the final stage of cystacanthus formation in the intermediate host, but remain connected with each other during later life. It can be assumed that the fragments of each giant tegument nucleus are united with each other to form an independent network that ensures the vital activity of the tegument, the volume of which increases many times during the period of intensive growth of the parasite in the definitive host.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"309 - 312"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semibalanus balanoides (L.) and Balanus crenatus Bruguière (Balanidae) Are Intermediate Hosts of Fimbriarioides intermedia (Fuhrmann, 1913) and Two Species of the Genus Microsomacanthus (Cestoda: Hymenolepidae), Parasites of Sea Ducks from the Atlantic Sector of the Arctic and Northern Pacific","authors":"K. V. Regel","doi":"10.1134/S1607672924701035","DOIUrl":"10.1134/S1607672924701035","url":null,"abstract":"<p>Metacestode infestation of <i>Semibalanus balanoides</i> (L., 1767) and <i>Balanus crenatus</i> Bruguiеre, 1789, collected in the Barents and White Seas and in the northern part of the Sea of Okhotsk in 2020 and 2021, respectively, was studied. A total of 313 <i>S. balanoides</i> from Mogilnaya Bay of Kildin Island (Barents Sea) and isolated mature wrinkled barnacles <i>B. crenatus</i>, two and four specimens from the Pechora Sea and Kandalaksha Bay of the White Sea, respectively, were examined in 2020. Metacestodes <i>Fimbriarioides intermedia</i> (Fuhrmann, 1913) (Cyclophyllidea, Hymenolepididae) were found in 1.0 ± 0.6% of <i>S. balanoides</i> in the Barents Sea with an invasion intensity (I. I.) of 2–5 specimens, and in one of two <i>B. crenatus</i> from the Pechora Sea (I. I. 15 specimens). For the first time, in both <i>B. crenatus</i> from the Pechora Sea, taken from the valves of the mussel <i>Mytilus edulis</i>, metacestodes <i>Microsomacanthus</i> sp. I (I. I. 13 and 20 specimens) with proboscis hooks 38–41 (39.4 ± 0.1) µm long and blades of 9.5–11 (10.7 ± 0.1) µm were obtained. One of four <i>B. crenatus</i> from the Kandalaksha Bay was infected with another <i>Microsomacanthus</i> sp. II (I. I. 19 specimens) with proboscis hooks 44.0–49.5 (45.7 ± 0.5) µm long and blades 14.0–16.0 (14.8 ± 0.07) µm long. A total of 362 <i>S. balanoides</i> were collected and dissected in Gizhiginskaya Bay of the northern part of the Sea of Okhotsk in 2021, of which 8.0 ± 1.4% were infected with <i>F. intermedia</i> metacestodes (I. I. 1–19 specimens). Study results of the infestation of <i>S. balanoides</i> on the Koni-Pyagin coast of the Sea of Okhotsk (according to the collections of 2006–2007) were supplemented and clarified. Description of metacestodes and the taxonomic affiliation of cysticercoids <i>Microsomacanthus</i> spp. are given.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"313 - 324"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of Echinococcus canadensis (Cestoda, Taeniidae) in Wolf from the Kirov Region","authors":"O. A. Loginova,&nbsp;S. E. Spiridonov,&nbsp;O. V. Maslennikova,&nbsp;L. A. Bukina","doi":"10.1134/S1607672924701011","DOIUrl":"10.1134/S1607672924701011","url":null,"abstract":"<p>This work presents the results of studying the molecular characteristics of parasitic tapeworms <i>Echinococcus canadensis</i>. The helminths were discovered during the autopsy of a wolf (<i>Canis lupus</i> Linnaeus, 1758) killed by hunters in the Kirov oblast in 2021. A molecular phylogenetic study was performed by analyzing the sequence of a fragment of the first subunit of the mitochondrial cytochrome oxidase gene (<i>Cox</i>I). It was found that the detected echinococci belong to the G10 genotype of <i>E. canadensis</i>, which is common in wolves in the northern territories of the Holarctic. We discovered four positions at which the substitutions characteristic only of this genotype are revealed. A substitution at one of the positions that is characteristic exclusively for the representatives of the G10 genotype found in Russia and Finland was also discovered.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"518 1","pages":"305 - 308"},"PeriodicalIF":0.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141490398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperleptinemia as a Marker of Various Phenotypes of Obesity and Overweight in Women with Rheumatoid Arthritis and Systemic Lupus Erythematosus","authors":"L.V. Kondratyeva,&nbsp;Yu. N. Gorbunova,&nbsp;T. A. Panafidina,&nbsp;T. V. Popkova","doi":"10.1134/S1607672924700893","DOIUrl":"10.1134/S1607672924700893","url":null,"abstract":"<p>The objective of the study was to identify different phenotypes of overweight in women with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) based on body mass index (BMI) and serum leptin levels, as well as to determine the frequencies of various metabolic disorders, hypertension, and cardiovascular complications (CVCs) in individual phenotypes. The study included 50 women with RA and 46 with SLE aged 18 to 65 years without a history of diabetes and fasting hyperglycemia. In all patients, the concentration of leptin was determined by ELISA, the concentration of insulin was determined by electrochemiluminescence analysis, and the HOMA-IR index was calculated. Hyperleptinemia was diagnosed at leptin concentrations &gt; 11.1 ng/mL; insulin resistance (IR), at HOMA-IR values ≥ 2.77. Three main phenotypes of overweight were distinguished: “classic” (BMI ≥ 25 kg/m² + hyperleptinemia), “healthy” (BMI ≥ 25 kg/m², without hyperleptinemia), “hidden” or “latent” (BMI &lt; 25 kg/m² + hyperleptinemia), as well as “normal weight” (BMI &lt; 25 kg/m², without hyperleptinemia). Patients with RA and SLE were similar in age (<i>p</i> = 0.4), disease duration (<i>p</i> = 0.2) and BMI (<i>p</i> = 0.5). Hyperleptinemia was found in 46% of women with RA and in 74% of women with SLE (<i>p</i> = 0.005), and IR was found in 10 and 22% of patients, respectively (<i>p</i> = 0.2). The “classic” phenotype of overweight was diagnosed in 30%, “healthy” in 8%, and “hidden” in 16% of cases with RA and in 44%, 0%, and 30% of cases with SLE, respectively. IR was found in 3% and hypertension in 6% of patients with “normal weight.” With the “classic” phenotype, IR (29%) and hypertension (66%) were more common than with “normal weight” (<i>p</i> &lt; 0.01 in all cases); with the “hidden” phenotype, significant differences were obtained only in hypertension frequency (45%; <i>p</i> = 0.0012), but not IR (18%). Three out of four women with a history of cardiovascular complications suffered from “classic” overweight, and one patient had a “normal weight.” In women with SLE up to 65 years of age, the frequency of hyperleptinemia, but not IR, is higher than in patients with RA. In both diseases, the “classic” overweight phenotype is most common. In RA, a “hidden” phenotype was detected less often than in SLE, at the same time, a “healthy” phenotype is not characteristic of SLE. The frequency of metabolic disorders and hypertension is low with the “normal weight” and “healthy” phenotype, high with the “classic” phenotype, and intermediate with the “hidden” phenotype.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"517 1","pages":"182 - 194"},"PeriodicalIF":0.8,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}