Doklady Biochemistry and Biophysics最新文献

{"title":"Antiosteoporosis and Bone Protective Effect of Phyllanthin Against Glucocorticoid-induced Osteoporosis in Rats via Alteration of HO-1/Nrf2 and RANK/RANKL/OPG Pathway","authors":"Shaosong Sun,&nbsp;Yilei Liu,&nbsp;Xiaofeng Liu,&nbsp;Panxiang Li","doi":"10.1134/S1607672924600866","DOIUrl":"10.1134/S1607672924600866","url":null,"abstract":"<div><p><b>Background. </b>Osteoporosis is a condition where bones weaken due to a loss in density and quality, making them fragile and more susceptible to fractures, even from minor stress or injury. In this experimental study, we scrutinized the antiosteoporosis effect of phyllanthin against glycocorticoid (GIOP) induced osteoporosis in rats.</p><p><b>Methods. </b>SD rats were used in this study and subcutaneous administration of DEX (3 mg/kg) was used for the induction of osteoporosis and rats were treated with phyllanthin and alendronate for 12 weeks. The body weight, femur mass, length, hormones, nutrients, antioxidant, cytokines and bone parameters were estimated. The mRNA expression of HO-1, Nrf2, RANK, RANKL and OPG were estimated.</p><p><b>Results. </b>Phyllanthin treatment significantly (<i>p</i> &lt; 0.001) improved the body weight, femur mass and femur length. Phyllanthin significantly (<i>p</i> &lt; 0.001) altered the level of hormones estrodiol, PTH; nutrients such as calcium, phosphorus, magnesium; Bone mineral content (BMC) and bone mineral density (BMD); Bone formation marker like ALP, TRAP, osteocalcin, β-CTX, BGP, cathepsin K, DPD; Bone parameters viz., Tb.N, BV/TV, Tb.sp, BS/BV, Tb.Th; Bone structure analysis includes maximum load, energy, stiffness, maximum stress, young’s modules; oxidative stress parameters such as TBARS, CAT, GPx, GSH, GR; cytokines such as TNF-α, IL-1β, IL-6, IL-10 and antioxidant marker such as HO-1 and Nrf2. Phyllanthin significantly (<i>P</i> &lt; 0.001) altered the mRNA expression of HO-1, Nrf2, RANK, RANKL and OPG.</p><p><b>Conclusion.</b> On the basis of result, we can say that phyllanthin exhibited the antiosteoporosis effect against glucocorticoid-induced osteoporosis in rats via alteration of HO-1/Nrf2 and RANK/RANKL/OPG pathway.</p></div>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"109 - 122"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of TRPM2 Channel in Doxorubicin-induced Cell Damage in Laryngeal Squamous Cancer Cells","authors":"Tarık Yağcı,&nbsp;Ramazan Çınar,&nbsp;Halil İbrahim Altıner,&nbsp;Rıza Dündar,&nbsp;Kenan Yıldızhan","doi":"10.1134/S1607672924601070","DOIUrl":"10.1134/S1607672924601070","url":null,"abstract":"<p>Laryngeal squamous cell carcinoma is a common type of head and neck cancer. This study investigated the role of the TRPM2 channel in doxorubicin (DOX)-induced cell damage in human laryngeal squamous cancer cells (Hep-2). Cells were exposed to various DOX concentrations and the appropriate dose was found. Then, TRPM2 antagonist ACA was treated. At the end of the study, cell viability test, Western blot and oxidative stress and inflammatory markers were examined. The results showed that TRPM2 channel expression increased with DOX administration, and DOX incubation in cells caused an increase in ROS, MDA, IL-1β, IL-6, and TNF-α levels, while GSH and GSH-Px levels decreased. Concurrent treatment with ACA attenuated these effects and reduced oxidative stress and inflammation. In addition, DOX-induced apoptosis markers including Casp-3, Casp-8, Casp-9, p53, and Bax were elevated, while Bcl-2 levels were decreased; ACA treatment reversed these changes. The study demonstrated that DOX treatment significantly enhances TRPM2 channel activation and ROS production in Hep-2 cells, thereby initiating apoptotic pathways that lead to cell death. Consequently, targeting the TRPM2 channel may represent a promising therapeutic strategy for treating laryngeal cancer.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"123 - 129"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alteration of the Catalytic Properties of the Epoxyalcohol Synthase CYP443D1 (NvEAS) of the Starlet Sea Anemone Nematostella vectensis as a Result of a Single Amino Acid Substitution","authors":"S. S. Gorina,&nbsp;N. V. Lantsova,&nbsp;Y. Y. Toporkova,&nbsp; A. N. Grechkin","doi":"10.1134/S160767292460057X","DOIUrl":"10.1134/S160767292460057X","url":null,"abstract":"<p>Cytochromes of the P450 superfamily are widespread in nature; they were found in all studied aerobic organisms. Although the degree of similarity between cytochromes P450 of different families is low, all enzymes of this superfamily have similar tertiary structures. In addition, all cytochromes P450, including enzymes of the CYP74 clan, contain substrate recognition sites in their sequences, which form the catalytic center. Initially, CYP74 enzymes were discovered in plants, where they are widespread and play an important role in the lipoxygenase cascade. Later, CYP74-like enzymes of other families were identified in different taxa, including animals. Based on the results of phylogenetic studies, structures, and catalytic mechanisms, they were combined along with the CYP74 family into the CYP74 clan. One of the CYP74 clan enzymes is the epoxyalcohol synthase NvEAS (CYP443D1) of the starlet sea anemone <i>Nematostella vectensis</i>. A mutant form of NvEAS with a P93G substitution, that acquired additional hydroperoxide lyase activity, was obtained by site-directed mutagenesis. Before this work, only the results of site-directed mutagenesis of enzymes of the CYP74 family, but not of the CYP74 clan, were described. Moreover, in this work, the transformation of epoxyalcohol synthase into hydroperoxide lyase is described for the first time. These results confirm the previously stated assumption about the evolution of CYP74 enzymes, namely the epoxyalcohol synthase – hydroperoxide lyase – allene oxide synthase – divinyl ether synthase pathway.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"42 - 48"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Retrodipeptide Analogue of Cholecystokinin Tetrapeptide (GB-115) and Phenazepam on the Behavior of Rhesus Monkeys under Isolation Conditions","authors":"Al. V. Panchenko,&nbsp;An. V. Panchenko,&nbsp;L. E. Pavlova,&nbsp;M. F. Timina,&nbsp;E. V. Cherkashina,&nbsp;L. G. Kolik,&nbsp; S. B. Seredenin","doi":"10.1134/S1607672924701254","DOIUrl":"10.1134/S1607672924701254","url":null,"abstract":"<p>Introduction. The developed domestic retrodipeptide analogue of cholecystokinin tetrapeptide (CCK) (N-(6-phenylhexanoyl)-glycyltryptophan amide, or compound GB-115) with antagonistic properties in relation to CCK1 receptors has anxiolytic activity previously shown in preclinical and clinical studies. \u0000<b>The aim of the study</b> was to evaluate the anxiolytic effect of GB-115 as a tablet form with subchronic oral administration in comparison with phenazepam in nonhuman primates. \u0000<b>Materials and methods.</b> The study was conducted on four male rhesus monkeys (<i>Macaca mulatta</i>) aged 5.7–6.7 years. After a 30-day adaptation to the conditions of individual caging, experiments were performed first with GB-115 (tablets of 0.001 g) and second with phenazepam (tablets of 0.0005 g). Both drugs were administered one at a time (7 days) and then two tablets each (7 days). Behavior was assessed by observing an object with registration according to the “Yes-No” principle of ethogram elements at the background periods, during the administration of the drugs, and after their withdrawal. The stress response was assessed using cortisol and dehydroepiandrosterone sulfate (DHEA-S) levels as biomarkers determined in the blood serum by enzyme immunoassay. \u0000<b>Results.</b> GB-115 (0.001 g each) and phenazepam (0.001 g each) decreased the stay of the animals in the upper part of the cage compared to the background period, indicating decrease in stress response. GB-115 (0.002 g each) reduced the cortisol/DHEA-S ratio. Phenazepam dose-dependently decreased the level of cortisol in the blood serum without affecting the content of DHEA-S. Under administration of phenazepam (0.001 g each), a decreased cortisol/DHEA-S ratio was also observed. \u0000<b>Conclusions.</b> A positive effect of GB-115 upon subchronic administration on the attenuation of the emotional stress response and the restoration of adaptive behavior in rhesus monkeys comparable to the effect of phenazepam was revealed, which confirms the possibility of using blockade of CCK1 receptors as one of the approaches for treating anxiety disorders.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"83 - 88"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Long-Term Cultivated In Vitro Willow Clones according to Chromosomal Analysis","authors":"O. S. Mashkina,&nbsp;T. M. Tabatskaya","doi":"10.1134/S1607672924701230","DOIUrl":"10.1134/S1607672924701230","url":null,"abstract":"<p>Creation and long-term in vitro maintenance of valuable genotype collection is one of the modern approach to conservation of valuable gene pool of woody plants. However, during prolonged cultivation, genetic variability of cells and tissues may accumulate and lead to the loss of valuable characteristics of parental plants. It is therefore important to assess the genetic (including cytogenetic) stability of collection clones. \u0000The purpose of this work was to study the karyotype features (number, ploidy level and chromosome size) of various willow clones (<i>S. dasyclados</i> Wimm., <i>S. viminalis</i> L., <i>S. purpurea</i> L., <i>S. caspica</i> Pall., х <i>S. palustris</i> Host.) under conditions of long-term in vitro storage. No such research has been conducted on willow so far. Nevertheless, there is evidence of significant influence of ploidy level on growth, productivity, and composition of wood for willow plants. \u0000Our study was based on the plants of five micropropagated willow clones rated as promising for plantation forestry. The plants used in the study were maintained in vitro for a long period (14 years) by rare subculturing (once in 5 months) on a hormone-free 1/2 WPM nutrient medium under standard cultivation conditions (25 ± 2°C, 16 h light/8 h dark, 2.0 klx). \u0000Throughout the entire in vitro cultivation period, the plants showed proper growth and development, high regeneration activity, and no visible signs of somaclonal variation. Willow is one of the rather difficult objects for karyotype analysis. The authors improved the method of preparation and analysis of specimens. During the long-term cultivation, the clones showed cytogenetic stability, maintaining the ploidy (2<i>n</i> = 2х = 38 or 2<i>n</i> = 4х = 76) and the mixoploid nature of the original plants. Data obtained gives an update on the sizes of chromosomes of clones with different ploidy. The absolute chromosome length for diploid clones varies from 0.8 to 2.1 μm; tetraploid, from 0.9 to 2.5 μm. There were no statistically significant differences in the average chromosome length between diploid and tetraploid clones. All studied willow clones during long-term (for 14 years) in vitro cultivation on a hormone-free nutrient media retain the karyotype of the respective species.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"67 - 73"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Omega-3 Docosahexaenoic Acid as a Promising Inducer of Ferroptosis: Dynamics of Action in Prostate and Colorectal Cancer Models","authors":"D. M. Olkhovik,&nbsp;M. O. Silkina,&nbsp;A. V. Razumovskaya,&nbsp;K. V. Klycheva,&nbsp;A. A. Fatkulin,&nbsp;T. A. Kulagin,&nbsp;S. V. Nikulin","doi":"10.1134/S160767292460132X","DOIUrl":"10.1134/S160767292460132X","url":null,"abstract":"<p>Ferroptosis is an iron-dependent form of programmed cell death (PCD) associated with lipid membrane peroxidation. It has gained attention in cancer research because some tumor cells that are resistant to other forms of PCD are sensitive to ferroptosis. Despite the significant amount of research on ferroptosis, the list of known inducers remains limited, creating opportunities to discover new compounds with clinical potential. Recent studies have shown that long-chain polyunsaturated fatty acids, such as omega-3 docosahexaenoic acid (DHA), can function as ferroptosis inducers. In this study, we examined the kinetics of ferroptosis in prostate and colorectal cancer cells under the influence of erastin and DHA. Differences in the kinetics and mechanisms of action were observed. Moreover, cells resistant to erastin were found to be sensitive to DHA, confirming the potential of further research into its use as an anticancer agent.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"25 - 28"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S160767292460132X.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parametric Search for Optimal Channel Shape for Swirling Blood Flow in the Heart and Main Vessels","authors":"Ya. E. Zharkov,&nbsp;A. V. Agafonov,&nbsp;Alex. Y. Gorodkov,&nbsp;L. A. Bockeria","doi":"10.1134/S1607672924701278","DOIUrl":"10.1134/S1607672924701278","url":null,"abstract":"<p>The study presents a numerical parametric investigation of flow structures in channels with a longitudinal-radial profile <i>zR</i>^<i>N</i> = Const and a spherical dome at the base. The goal of the study was to examine the flow structures in these channels depending on the exponent <i>N</i> of the profile and the height of the dome, to determine the conditions that provide optimal centripetal swirling flow, analogous to blood flow in the heart chambers and major vessels. The investigation was conducted using a comparative analysis of flow structures in channel configurations <i>zR</i>^<i>N</i> = Const, carried out in two stages. In the first stage, the convergence parameter <i>N</i> was varied from 1.25 to 2.75 to identify the value that ensures optimal flow conditions. In the second stage, for the established value of <i>N</i>, the dome height was varied from 2.5 to 15 mm to identify the beneficial effects associated with its presence. The method of investigation involved numerical modeling in a steady-state regime. The results of the study on the influence of the convergence parameter revealed that the profile <i>zR</i>² = Const provides optimal conditions for the formation of swirling flow with minimal specific losses and a uniform distribution of velocity gradients. This channel configuration also showed the best agreement with the analytical solutions for Burgers’ vortex, confirming its effectiveness in the static approximation of flows. The parametric investigation of dome height indicated that an optimal dome height of 7 mm contributes to the smoothing of velocity gradients and the reduction of viscous losses due to the optimal enhancement of the centripetal swirling flow scale.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"6 - 19"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Triterpenoids in the Composition of Fat-Containing Products on the State of the Liver of Laboratory Animals with Acute Toxic Hepatitis","authors":"E. V. Averyanova,&nbsp;M. N. Shkolnikova,&nbsp;O. V. Chugunova,&nbsp;O. N. Mazko","doi":"10.1134/S1607672924701229","DOIUrl":"10.1134/S1607672924701229","url":null,"abstract":"<p>One of the principles of prevention and non-drug treatment of liver diseases, including hepatitis of various etiologies, is the normalization of the diet, including the use of daily diet foods with physiologically active ingredients, in particular betulin, which helps to reduce metabolic and oxidative processes within liver cells. \u0000<b>The aim of the work</b> was to evaluate the in vivo effect of triterpene alcohol betulin Roth isolated from the bark of birch <i>Betula pendula</i> Roth. added to fat-containing products (for example, mayonnaise) on the biochemical parameters of blood and the morphological structure of the liver of rats with initiated acute toxic hepatitis. \u0000<b>Materials and methods.</b> The composition of mayonnaise (67% fat) was developed with the addition of 0.2% betulin solution in vegetable oil in an amount of 6%. The hepatoprotective activity of betulin in mayonnaise samples was studied on a model of toxic hepatitis initiated by carbon tetrachloride in male Wistar rats. Disturbance of metabolic and oxidative processes in the liver cells of animals was assessed by biochemical parameters of blood plasma and the enzyme activity of the liver sample. The effect of experimental samples of mayonnaise with betulin on the morphological structure of the liver of rats with toxic hepatitis was evaluated by microscopy of prepared sections of liver tissue. \u0000<b>Results.</b> The preventive efficacy of betulin in mayonnaise was confirmed in an in vivo experiment: its administration weakens the hepatotoxic effect of carbon tetrachloride, as indicated by a significant decrease in the activity of ALT, AST, and alkaline phosphatase enzymes in the blood plasma of animals MG-1 compared with the blood of rats CG-2—ALT by 22.1%, AST by 21.9%, and alkaline phosphatase by 42.5%. A 13.9% decrease in GGT activity in MG-1 animals indicates a more intensive nitrogen metabolism with a decrease in the level of hepatocyte cytolysis compared to animals of the experimental MG-2 group. It was shown that the introduction of mayonnaise with betulin in a dose of 1 ml into the diet of animals, which corresponds to 35 mg of betulin, prevented the development of cytolytic syndrome and suppressed peroxidation by direct neutralization of free radicals. \u0000<b>Conclusions.</b> The experiment showed a significant decrease in cholesterol, glucose, and enzyme activity of the liver sample and normalization of albumin and triglyceride concentrations in animals of the experimental group in comparison with similar indices in the control group. Betulin in mayonnaise contributed to the antioxidant protection of the body, expressed in the effect on the activity of antioxidant enzymes that are involved in the destruction of organic peroxides, especially lipid peroxides, disturbing the integrity of cell membranes.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"58 - 66"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic Shifts in Multipotent Mesenchymal Stromal Cells during Microgravity Simulation","authors":"D. A. Yakubets,&nbsp; L. B. Buravkova","doi":"10.1134/S1607672924601148","DOIUrl":"10.1134/S1607672924601148","url":null,"abstract":"<p>One of the most obvious manifestations of the negative impact of space flight factors on the human physiology is osteopenia. With the active development of manned space flights and the increase in the duration of humans' persistence in weightlessness, there is a growing need to understand the mechanisms of changes occurring at the cellular level involved in the replenishment of bone tissue. Using the RNA sequencing method, changes in the transcriptome profile of MMSCs were studied after a 5-day simulation of the microgravity effects. During the analysis, a pronounced downregulation of genes, which products are involved in processes associated with cell proliferation, in particular, in the mitotic phase of the cell cycle, was found in the experimental group of cells. These shifts in the transcriptional profile of MMSCs were confirmed using fluorescence microscopy. The results obtained indicate a decrease in the proliferative activity of MMSCs cultured under conditions of simulated microgravity for 5 days.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"520 1","pages":"29 - 33"},"PeriodicalIF":0.8,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a Panel of Biomarkers for Differential Diagnosis of Multiple Sclerosis","authors":"L. A. Ovchinnikova,&nbsp;S. S. Dzhelad,&nbsp;T. O. Simaniv,&nbsp;M. N. Zakharova,&nbsp;Y. A. Lomakin,&nbsp; A. G. Gabibov,&nbsp; S. N. Illarioshkin","doi":"10.1134/S160767292460060X","DOIUrl":"10.1134/S160767292460060X","url":null,"abstract":"<p>Demyelinating diseases are a group of heterogeneous pathologies that affect the nervous system and reduce the quality of life. One of such diseases is multiple sclerosis (MS), an inflammatory autoimmune neurodegenerative disease of the central nervous system (CNS). At the initial stages, MS can mimic some infectious, neoplastic, genetic, metabolic, vascular, and other pathologies. Accurate differential diagnosis of this disease is important to improve the quality of life of patients and reduce possible irreversible damage to the central nervous system. In this work, we confirmed the possibility of using our previously proposed candidate panel of MS biomarkers to distinguish MS from neuromyelitis optica spectrum disorder (NMOSD) and amyotrophic lateral sclerosis (ALS). We have shown that our proposed panel (SPTAN1_601-644 + PRX_451-494 + PTK6_301-344 + LMP1_285-330) allows us to distinguish MS from ALS (AUC = 0.796) and NMOSD (AUC = 0.779).</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"519 1","pages":"593 - 596"},"PeriodicalIF":0.8,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S160767292460060X.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}