Doklady Biochemistry and Biophysics最新文献_第7页

20-Hydroxyecdysone Modulates Gene-Specific Immune Response to Metarhizium anisopliae Infection in Drosophila melanogaster S2 Cell Culture 20-羟基脱皮激素调节黑尾果蝇S2细胞对绿僵菌感染的基因特异性免疫反应。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-12-02 DOI: 10.1134/S1607672925601398

M. Ghassah, Y. A. Ulianova, P. G. Georgiev, Y. V. Shidlovskii, Z. M. Kachaev

{"title":"20-Hydroxyecdysone Modulates Gene-Specific Immune Response to Metarhizium anisopliae Infection in Drosophila melanogaster S2 Cell Culture","authors":"M. Ghassah, Y. A. Ulianova,  P. G. Georgiev, Y. V. Shidlovskii, Z. M. Kachaev","doi":"10.1134/S1607672925601398","DOIUrl":"10.1134/S1607672925601398","url":null,"abstract":"The interplay between innate immunity and other signaling pathways remains a central focus in immunological research, with considerable ongoing investigation. Of particular interest are studies exploring the influence of the hormonal system on the innate immunity of Drosophila melanogaster. In this study, we performed a comprehensive analysis of the combined effects of the insect hormone 20-hydroxyecdysone (20E) and spores from the fungus Metarhizium anisopliae on the innate immune response in D. melanogaster S2 cell culture, addressing this interaction for the first time. Our results demonstrate that, compared to cells exposed solely to M. anisopliae spores, pretreatment with 20E followed by fungal challenge led to a reduction in the transcription of antimicrobial peptide genes CecropinA1 and Drosocin. In contrast, expression of the Metchnikowin (Mtk) gene was upregulated. No significant alterations were observed in the transcription levels of Drosomycin or in genes encoding key receptors, transcription factors, or other components of innate immune signaling pathways. Furthermore, knockdown of the transcription factor Relish markedly decreased Mtk expression, highlighting its central role in hormone-modulated antifungal immunity. These findings reveal complex hormonal–immune crosstalk that differentially regulates AMP gene expression in Drosophila.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"558 - 561"},"PeriodicalIF":0.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MLE/DHX9 without Helicase Activity Activates Constitutive Expression of Nuclear Receptor Genes in Drosophila melanogaster 无解旋酶活性的MLE/DHX9激活黑腹果蝇核受体基因的组成表达。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-12-02 DOI: 10.1134/S1607672925601180

I. A. Zolin, A. A. Grigel, S. G. Georgieva, J. V. Nikolenko

{"title":"MLE/DHX9 without Helicase Activity Activates Constitutive Expression of Nuclear Receptor Genes in Drosophila melanogaster","authors":"I. A. Zolin, A. A. Grigel,  S. G. Georgieva, J. V. Nikolenko","doi":"10.1134/S1607672925601180","DOIUrl":"10.1134/S1607672925601180","url":null,"abstract":"MLE/DHX9 is a DNA/RNA helicase that performs important functions in gene expression regulation in eukaryotes. However, the specific role of MLE and the mechanisms by which this regulation is carried out remain poorly understood. This work is devoted to the study of the effect of MLE on the constitutive expression of nuclear receptor genes in D. melanogaster. The nuclear receptors Eip75B, DHR3, and Hr4 are evolutionarily conserved and have orthologs in humans. In D. melanogaster, Eip75B, DHR3, and Hr4 are activated in the ecdysone cascade, but they are also expressed at a stable level in various tissues and at various stages of development. The experiments were carried in vivo at the imago stage in females and in S2 cell culture. Taken together, the results indicate that MLE is involved in activating the expression of these genes. At the same time, the helicase activity of MLE is not necessary for activation. The results obtained extend knowledge about the functions of MLE beyond dosage compensation, as potentially conserved in evolution, and contribute to understanding the mechanisms of regulation of nuclear receptor expression.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"525 1","pages":"527 - 531"},"PeriodicalIF":0.7,"publicationDate":"2025-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145652906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Antitumor Effect of High-Energy Protons, Helium Ions, and Carbon during Irradiation of Ehrlich Adenocarcinoma Ascites Cells Ex Vivo 体外评价高能质子、氦离子和碳辐照埃利希腺癌腹水细胞的抗肿瘤作用。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-12-02 DOI: 10.1134/S1607672925601209

V. E. Balakin, N. S. Strelnikova, O. M. Rozanova, E. N. Smirnova, T. A. Belyakova, A. V. Smirnov

引用次数: 0

Structural and Functional Features of Melatonin Biosynthesis Genes SNAT1 and SNAT2 in Tomato and Garlic Plants 番茄和大蒜褪黑素合成基因SNAT1和SNAT2的结构和功能特征

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-12-02 DOI: 10.1134/S1607672925601349

O. K. Anisimova, A. V. Shchennikova, E. Z. Kochieva

引用次数: 0

Effect of Femtosecond Laser Radiation on Cognitive Abilities in Mice 飞秒激光辐射对小鼠认知能力的影响。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-11-20 DOI: 10.1134/S1607672925601234

G. R. Ivanitskii, S. S. Sorokina, A. R. Dyukina, V. I. Yusupov

引用次数: 0

Fatty Acid Composition of the European Pearl Mussel Margaritifera margaritifera from Different Rivers of the White Sea Basin 白海盆地不同河流欧洲珍珠贻贝的脂肪酸组成。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-11-20 DOI: 10.1134/S1607672925601283

S. N. Khurtina, V. P. Voronin, E. P. Ieshko, N. N. Fokina, D. A. Efremov, S. A. Murzina

{"title":"Fatty Acid Composition of the European Pearl Mussel Margaritifera margaritifera from Different Rivers of the White Sea Basin","authors":"S. N. Khurtina, V. P. Voronin, E. P. Ieshko, N. N. Fokina, D. A. Efremov, S. A. Murzina","doi":"10.1134/S1607672925601283","DOIUrl":"10.1134/S1607672925601283","url":null,"abstract":"The fatty acid (FA) composition of the mantle, foot (muscle), and hepatopancreas of the European pearl mussel Margaritifera margaritifera from the Kem River (estuary), the Ukhta River, and the Vozhma River (Republic of Karelia, Russia) was studied for the first time. All tissues of the pearl mussel from the studied rivers were shown to have a higher content of saturated and polyunsaturated FAs compared to monounsaturated ones, which may be due to the post-spawning period. In the hepatopancreas of the pearl mussel, a predominance of polyunsaturated FAs due to the FAs of n-3 family, as well as a higher amount of 16:1(n-7), 18:1(n-7), 18:3(n-3), 20:5(n-3), and 22:6(n-3) FAs and a lower amount of 16:0, 18:0, 18:1(n-9), 20:1(n-11), and 20:4(n-6) FAs compared to the foot and mantle was shown. An important distinctive feature of M. margaritifera is the high amount of 20:1(n-11) FA. Mollusks from the Kem River estuary were distinguished by a higher content of 18:0 and 20:5(n-3) FAs in all the studied tissues and a lower content of 18:1(n-7), 18:2(n-6), and 22:6(n-3) FAs. The obtained data are important for monitoring work to assess the state of pearl mussel populations in different rivers of the European North (in particular, using physiological and biochemical indices).","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"524 1","pages":"519 - 526"},"PeriodicalIF":0.7,"publicationDate":"2025-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145562104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of the Protein-Opener of the Desmoglein Contacts on the Accumulation of Targeted Nanoagents in HER2-Positive Solid Tumors 在her2阳性实体瘤中，蛋白开片蛋白接触对靶向纳米药物积累的影响。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925600551

G. M. Proshkina, E. I. Shramova, A. B. Mirkasymov, E. V. Serova, S. M. Deyev

引用次数: 0

Expression Level of Carotenoid Biosynthesis Genes in Leaves Is Associated with Cold Tolerance of Zea mays L. Plants 玉米叶片类胡萝卜素生物合成基因表达水平与抗寒性的关系

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925600502

D. Kh. Arkhestova, E. Z. Kochieva, A. V. Shchennikova

引用次数: 0

Effects of LYS-ARG-ARG-LYS-PRO-GLY-PRO Peptide, Warfarin, and Acetylsalicylic Acid on Lipid Metabolism in Rats with Metabolic Syndrome LYS-ARG-ARG-LYS-PRO-GLY-PRO肽、华法林和乙酰水杨酸对代谢综合征大鼠脂质代谢的影响。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925600459

T. Yu. Obergan, N. F. Myasoedov, T. A. Shubina, M. E. Grigorjeva, L. A. Lyapina

引用次数: 0

Differential Methylation of the Genes PAX8 and GATA4 Alters Their Expression in the Heart during Hypertrophic Cardiomyopathy 基因PAX8和GATA4的差异甲基化改变了它们在肥厚性心肌病心脏中的表达。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925601131

I. S. Kiselev, E. A. Dashtieva, M. V. Pisklova, O. S. Chumakova, A. S. Zotov, M. R. Kabilov, O. A. Baturina, D.A. Zateyshchikov, O. O. Favorova

{"title":"Differential Methylation of the Genes PAX8 and GATA4 Alters Their Expression in the Heart during Hypertrophic Cardiomyopathy","authors":"I. S. Kiselev, E. A. Dashtieva, M. V. Pisklova, O. S. Chumakova, A. S. Zotov, M. R. Kabilov, O. A. Baturina, D.A. Zateyshchikov, O. O. Favorova","doi":"10.1134/S1607672925601131","DOIUrl":"10.1134/S1607672925601131","url":null,"abstract":"Hypertrophic cardiomyopathy (HCM) is the most common hereditary heart disease with a prevalence ranging from 1 : 500 to 1 : 200 individuals. The development and clinical presentation of HCM do not always conform to the traditional view of its monogenic inheritance pattern. One key to addressing this issue may lie in identifying epigenetic mechanisms regulating gene expression, particularly DNA methylation, involved in the pathogenesis of the disease and modifying its course. Using previously obtained whole-genome data, we identified four extended genomic regions with reduced methylation levels in the myocardium of patients with HCM. These include region chr2:113993204-113994075, located within the transcribed area of the PAX8 gene, as well as three other regions (chr6:31148369-31148577, chr8:11565217-11567212, and chr8:22132791-22133357), which are associated with promoters of genes PSORS1C3, GATA4, and PIWIL2, respectively. We demonstrated altered expression of PAX8 and GATA4 genes containing one each of these four mentioned regions. Our findings will expand the currently very limited understanding of the unique features of epigenetic regulation in this condition.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"524 1","pages":"491 - 497"},"PeriodicalIF":0.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0