Doklady Biochemistry and Biophysics最新文献_第8页

Variant Analysis of miRNA Regulatory Genes in 35 Sporadic Lung Carcinoma Tumors 35 例散发性肺癌肿瘤中 miRNA 调控基因的变异分析

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI: 10.1134/S1607672924600052

Özkan Bağcı, Ebru Marzioğlu Özdemir, Batuhan Şanlıtürk

{"title":"Variant Analysis of miRNA Regulatory Genes in 35 Sporadic Lung Carcinoma Tumors","authors":"Özkan Bağcı, Ebru Marzioğlu Özdemir, Batuhan Şanlıtürk","doi":"10.1134/S1607672924600052","DOIUrl":"10.1134/S1607672924600052","url":null,"abstract":"Lung cancer is one of the cancer types with the highest mortality worldwide. The most frequently mutated genes known to be clinically important in lung cancers are EGFR, BRAF, and KRAS genes. Therefore, the therapeutic agents developed are directed against variants that cause over-activation of the EGFR–KRAS–BRAF–BRAF–MEK/ERK signalling pathway. However, different responses of patients to Tyrosine Kinase Inhibitors (TKIs) suggest that new prognostic biomarkers should be defined and epigenetic mechanisms may be related to this situation. Methods: In this study, sequence analyses of AGO2, DICER, and DROSHA genes involved in miRNA biogenesis and EGFR, KRAS, and BRAF genes were performed in 35 patients with sporadic lung cancer. Results: We found variations in genes involved in miRNA biogenesis that have not been previously reported in the literature. In addition, we found 4 different variants in the EGFR gene that have been described in the literature. In addition, a statistically significant association was found between the presence of mutations in at least one of the genes involved in miRNA biogenesis and metastasis (p:0.02). Conclusions: In conclusion, genomic dysregulation of key miRNA biogenesis genes may be one of the possible reasons for the differential response of patients to therapeutic agents and the development of metastasis in EGFR wild type tumours.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"513 1 supplement","pages":"S1 - S7"},"PeriodicalIF":0.8,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140108706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-tumor Effect of High Doses of Carbon Ions and X-Rays during Irradiation of Ehrlich Ascites Carcinoma Cells Ex Vivo 大剂量碳离子和 X 射线辐照艾氏腹水癌细胞的体内抗肿瘤效应

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI: 10.1134/S1607672924700765

V. E. Balakin, T. A. Belyakova, O. M. Rozanova, E. N. Smirnova, N. S. Strelnikova, E. A. Kuznetsova

{"title":"Anti-tumor Effect of High Doses of Carbon Ions and X-Rays during Irradiation of Ehrlich Ascites Carcinoma Cells Ex Vivo","authors":"V. E. Balakin, T. A. Belyakova, O. M. Rozanova, E. N. Smirnova, N. S. Strelnikova, E. A. Kuznetsova","doi":"10.1134/S1607672924700765","DOIUrl":"10.1134/S1607672924700765","url":null,"abstract":"The effect of carbon ions (12C) with the energy of 400 MeV/nucleon on the dynamics of induction and growth rate of solid tumors in mice under irradiation of Ehrlich ascites carcinoma cells (EAC) ex vivo at doses of 5–30 Gy relative to the action of equally effective doses of X-ray radiation was studied. The dynamics of tumor induction under the action of 12C and X-rays had a similar character and depended on the dose during 3 months of observation. The value of the latent period, both when irradiating cells with 12C and X-ray, increased with increasing dose, and the interval for tumor induction decreased. The rate of tumor growth after ex vivo irradiation of EAC cells was independent of either dose or type of radiation. The dose at which EAC tumors are not induced within 90 days was 30 Gy for carbon ions and 60 Gy for X-rays. The value of the relative biological effectiveness of carbon ions, calculated from an equally effective dose of 50% probability of tumors, was 2.59.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"513 1 supplement","pages":"S30 - S35"},"PeriodicalIF":0.8,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140108655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Level of Anti-Viral Antigen-Specific Antibodies to EBNA-1 in the Serum of MS Patients Does not Depend on the Severity of the Disease 多发性硬化症患者血清中的 EBNA-1 抗病毒抗原特异性抗体水平与病情严重程度无关

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI: 10.1134/S1607672924700753

L. A. Ovchinnikova, S. S. Dzhelad, T. O. Simaniv, M. N. Zakharova, A. G. Gabibov, Y. A. Lomakin

引用次数: 0

Study of the Association of Ouib and Nom with Heterochromatin in Drosophila melanogaster 黑腹果蝇中 Ouib 和 Nom 与异染色质的关联研究

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI: 10.1134/S1607672924700741

Y. V. Pekina, V. A. Babosha, P. G. Georgiev, A. A. Fedotova

引用次数: 0

Experimental and Model Study of a Swirling Fluid Flow in a Converging Channel As a Simulation of Blood Flow in the Heart and Aorta 作为心脏和主动脉血流模拟的汇流槽中漩涡流的实验和模型研究

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI: 10.1134/S1607672924700777

Y. E. Zharkov, S. T. Zhorzholiani, A. A. Sergeev, A. V. Agafonov, A. Y. Gorodkov, L. A. Bockeria

{"title":"Experimental and Model Study of a Swirling Fluid Flow in a Converging Channel As a Simulation of Blood Flow in the Heart and Aorta","authors":"Y. E. Zharkov, S. T. Zhorzholiani, A. A. Sergeev, A. V. Agafonov, A. Y. Gorodkov,  L. A. Bockeria","doi":"10.1134/S1607672924700777","DOIUrl":"10.1134/S1607672924700777","url":null,"abstract":"Study of swirling flows in channels corresponding to the static approximation of flow channels of the heart and major vessels with a longitudinal–radial profile zR2 = const and a concave streamlined surface at the beginning of the longitudinal coordinate has been carried out. A comparative analysis of the flow structure in channel configurations zRN = const, where N = –1, 1, 2, 3, in the absence and presence of a concave surface was carried out. The numerical modeling was compared with the results of hydrodynamic experiments on the flow characteristics and the shape of the flow lines. The numerical model was used to determine the velocity structure, viscous friction losses, and shear stresses. Numerical modeling of steady-state flows for channels without a concave surface showed that in the channel zR2 = const there is a stable vortex flow structure with the lowest viscous friction losses. The presence of a concave surface of sufficient size significantly reduces viscous friction losses and shear stresses in both the steady state and pulsed modes.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"513 1 supplement","pages":"S36 - S52"},"PeriodicalIF":0.8,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140108656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRPF19 mRNA Encodes a Small Open Reading Frame That Is Important for Viability of Human Cells PRPF19 mRNA 编码一个小型开放阅读框，对人类细胞的存活率非常重要。

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-03-12 DOI: 10.1134/S1607672923700722

N. M. Shepelev, A. O. Kurochkina, O. A. Dontsova, M. P. Rubtsova

引用次数: 0

Regulation of Intestinal Flora and Immune Response by Cyanidin Exhibits Protective Effect against Type-2 Diabetes in Rat Model 矢车菊素调节肠道菌群和免疫反应对大鼠模型中的 2 型糖尿病有保护作用

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-02-20 DOI: 10.1134/S1607672923600422

Qingyan Lu, Qiannan Yan, Xiaojie Li

{"title":"Regulation of Intestinal Flora and Immune Response by Cyanidin Exhibits Protective Effect against Type-2 Diabetes in Rat Model","authors":"Qingyan Lu, Qiannan Yan, Xiaojie Li","doi":"10.1134/S1607672923600422","DOIUrl":"10.1134/S1607672923600422","url":null,"abstract":"In the current study the effects of metformin and cyanidin on the immune system and intestinal flora in rats with type-2 diabetes was investigated. The findings showed that metformin or cyanidin treatment considerably reduced the rise in body weight and glucose levels induced by type-2 diabetes. The type-2 diabetic rats’ glucose tolerance was significantly increased by cyanidin administration comparable to that of metformin. Cyanidin administration resulted in a significant reduction in serum cholesterol and low-density lipoprotein (LDL) levels in rats with type-2 diabetes. Treatment with cyanidin significantly increased the ratio of high-density lipoprotein to low-density lipoprotein in type-2 diabetes rats. Cyanidin administration significantly raised the ratio of Firmicutes to Bacteroidetes in the fecal samples of type-2 diabetic rats compared to the model group. In comparison to the model group, it also significantly raised the levels of Lactobacillus intestinalis, Lactobacillus gasseri, and Lactobacillus reuteri in the type-2 diabetes rats. In type-2 diabetes rat fecal samples, the abundance of Christensenellaceae significantly increased while Enterobacteriaceae and Proteobacteria were found to decrease upon cyanidin administration. Furthermore, cyanidin administration to the rats with type-2 diabetes significantly improved the glucose homeostasis. In conclusion, the study demonstrates that cyanidin enhances glucose homeostasis in rats with type-2 diabetes, potentially through controlling intestinal flora. Thus, cyanidin may be looked into more as a possible therapeutic agent for type 2 diabetes.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"513 1 supplement","pages":"S67 - S74"},"PeriodicalIF":0.8,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Description of the N-Protein of the SARS-CoV-2 Virus Degradation in Cells Stably Expressing It under the Influence of New Modular Nanotransporters 新型模块化纳米转运体影响下稳定表达 SARS-CoV-2 病毒的细胞中 N 蛋白降解的定量描述

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-02-20 DOI: 10.1134/S1607672923700709

Y. V. Khramtsov, A. V. Ulasov, T. N. Lupanova, G. P. Georgiev, A. S. Sobolev

引用次数: 0

Modular Nanotransporters Capable of Causing Intracellular Degradation of the N-Protein of the SARS-CoV-2 Virus in A549 Cells with Temporary Expression of This Protein Fused with a Fluorescent Protein mRuby3 在 A549 细胞中临时表达融合了荧光蛋白 mRuby3 的 SARS-CoV-2 病毒 N 蛋白的模块化纳米转运体可引起该蛋白的胞内降解

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-02-20 DOI: 10.1134/S1607672923700710

Y. V. Khramtsov, A. V. Ulasov, T. N. Lupanova, G. P. Georgiev, A. S. Sobolev

引用次数: 0

Effects of Chromatin Structure Modifiers on the trans-Acting Heterochromatin Position Effect in Drosophila melanogaster 染色质结构修饰剂对黑腹果蝇反式异染色质位置效应的影响

IF 0.8 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2024-02-20 DOI: 10.1134/S160767292470073X

A. A. Solodovnikov, S. A. Lavrov, A. S. Shatskikh, V. A. Gvozdev

{"title":"Effects of Chromatin Structure Modifiers on the trans-Acting Heterochromatin Position Effect in Drosophila melanogaster","authors":"A. A. Solodovnikov, S. A. Lavrov, A. S. Shatskikh,  V. A. Gvozdev","doi":"10.1134/S160767292470073X","DOIUrl":"10.1134/S160767292470073X","url":null,"abstract":"The heterochromatin position effect is manifested in the inactivation of euchromatin genes transferred to heterochromatin. In chromosomal rearrangements, genes located near the new eu-heterochromatin boundary in the rearrangement (cis-inactivation) and, in rare cases, genes of a region of the normal chromosome homologous to the region of the eu-heterochromatin boundary of the chromosome with the rearrangement (trans-inactivation) are subject to inactivation. The In(2)A4 inversion is able to trans-inactivate the UAS-eGFP reporter gene located on the normal chromosome. We knockdown a number of chromatin proteins using temperature-controlled RNA interference and investigated the effect of knockdown on trans-inactivation of the reporter. We found suppression of trans-inactivation by knockdowns of Su(var)2-HP2, a protein that binds to the key heterochromatin protein HP1a, SAYP, a subunit of the chromatin remodelling complex, and Eggless histone methyltransferase (SETDB1), which introduces a H3K9me3 histone mark, recognized by the HP1a protein. The method of studying the effects of gene knockdown on heterochromatin position effects presented in this work is of independent methodological interest.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"513 1 supplement","pages":"S75 - S81"},"PeriodicalIF":0.8,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139911762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0