Doklady Biochemistry and Biophysics最新文献_第8页

TREX-2 mRNA Export Complex Interacts with HLB Component FLASH and Is Recruited to Processed Histone mRNAs TREX-2 mRNA输出复合物与HLB成分FLASH相互作用，并被招募到加工的组蛋白mRNA中。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925601167

M. M. Kurshakova, Y. A. Yakusheva, S. G. Georgieva

引用次数: 0

A Syngenic Mouse Model of Breast Cancer Expressing Human ERBB2 and NanoLuc Luciferase Genes 表达人ERBB2和NanoLuc荧光素酶基因的乳腺癌小鼠同基因模型

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925601155

E. I. Shramova, S. M. Deyev, G. M. Proshkina

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Two RNA-Binding Regions of PCID2, a Subunit of the TREX-2 mRNA Nuclear Export Complex, Competitively Interact with the 3' Noncoding Region of ras2 mRNA TREX-2 mRNA核输出复合体的亚基PCID2的两个rna结合区与ras2 mRNA的3'非编码区竞争性相互作用。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925600563

Y. A. Vdovina, S. G. Georgieva, D. V. Kopytova

{"title":"Two RNA-Binding Regions of PCID2, a Subunit of the TREX-2 mRNA Nuclear Export Complex, Competitively Interact with the 3' Noncoding Region of ras2 mRNA","authors":"Y. A. Vdovina,  S. G. Georgieva, D. V. Kopytova","doi":"10.1134/S1607672925600563","DOIUrl":"10.1134/S1607672925600563","url":null,"abstract":"PCID2 protein is a subunit of the eukaryotic complex TREX-2, which is responsible for nuclear export of mRNA. PCID2 plays an important role in the complex, being responsible for the recognition and binding of the mRNA molecule. PCID2 of D. melanogaster interacts with a region of ras2 (fr4_2) mRNA and has two interaction sites located in its PCI domain: the M-PCID2 region, which non-specifically binds to mRNA, and the C-terminal part (C-PCID2), which specifically recognizes the ras2 fr4_2 mRNA sequence. At the same time, specific binding to C-PCID2 requires a preliminary nonspecific interaction with M-PCID2. It remains unclear how the transition from primary nonspecific interaction to specific interaction occurs: whether both regions interact with RNA simultaneously or whether the nonspecific interaction is required only at the first step for subsequent specific binding. This study showed that the binding of M-PCID2 and C-PCID2 to ras2 fr4_2 RNA is competitive. M-PCID2 binds more efficiently and displaces C-PCID2 from the complex with the ras2 mRNA fragment. Thus, additional factors are required to replace the M-PCID2 contact by C-PCID2 during the interaction of the full-length PCID2 protein with ras2 mRNA. We also showed that point mutations in M-PCID2 that disrupt the interaction of the full-length PCID2 with RNA result in a greater association of M-PCID2 with RNA. It is likely that the increased affinity of M-PCID2 for RNA disrupts the ability to replace M-PCID2 with C-PCID2 within the full-length PCID2.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"523 1","pages":"439 - 443"},"PeriodicalIF":0.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic Rearrangement of the Heart Rhythm in the Process of Organism Aging: Spectral Analysis 机体衰老过程中心律的系统重排：谱分析。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925600393

A. A. Grinevich, N. K. Chemeris

引用次数: 0

Interdomain Interactions of the PCID2 Protein, One of the Subunits of the TREX-2 mRNA Export Complex in Drosophila melanogaster 黑腹果蝇TREX-2 mRNA输出复合物亚基之一PCID2蛋白的结构域间相互作用

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925600575

Y. A. Vdovina, S. G. Georgieva, D. V. Kopytova

{"title":"Interdomain Interactions of the PCID2 Protein, One of the Subunits of the TREX-2 mRNA Export Complex in Drosophila melanogaster","authors":"Y. A. Vdovina,  S. G. Georgieva, D. V. Kopytova","doi":"10.1134/S1607672925600575","DOIUrl":"10.1134/S1607672925600575","url":null,"abstract":"The TREX-2 complex is responsible for the export of mRNA from the nucleus to the cytoplasm and consists of four proteins. It was recently shown that the PCID2 subunit of TREX-2 is responsible for the specific recognition of mRNA by the TREX-2 complex. The majority of the protein contains the PCI domain, which has surfaces for RNA binding. The PCI domain includes the central region of the protein, which has a surface for non-specific RNA binding, M-PCID2, and the C-terminal part of the PCI domain and the C-terminal part of the protein, C-PCID2, which has a surface for specific RNA recognition. The N-terminal part of PCID2 contains a region whose function is unknown. Since the TREX-2 complex binds to only a specific mRNA and only at a specific stage, we hypothesized that the N-terminal part of PCID2 might modulate the binding of C-PCID2 to RNA by binding to it and covering its RNA-binding domain. We showed that the N-terminal region interacts with C-PCID2. The binding of C-PCID2 to RNA in this case is not impaired. In addition, the binding of C-PCID2 to RNA does not disrupt its interaction with the N-terminal part of the protein (N-PCID2). Thus, C-PCID2 can interact with N-PCID2 and RNA by different surfaces. This intrinsic interaction is probably necessary at one of the stages of functioning of the TREX-2 complex.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"523 1","pages":"458 - 461"},"PeriodicalIF":0.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MLE/DHX9 Helicase Activity Is Required to Regulate the Expression of a Number of Tissue-Specific Genes on Chromosome 4 in Drosophila melanogaster MLE/DHX9解旋酶活性调节黑腹果蝇4号染色体上一些组织特异性基因的表达。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-10-13 DOI: 10.1134/S1607672925601222

I. A. Zolin, A. A. Grigel, S. G. Georgieva, A. N. Krasnov, J. V. Nikolenko

{"title":"MLE/DHX9 Helicase Activity Is Required to Regulate the Expression of a Number of Tissue-Specific Genes on Chromosome 4 in Drosophila melanogaster","authors":"I. A. Zolin, A. A. Grigel,  S. G. Georgieva, A. N. Krasnov, J. V. Nikolenko","doi":"10.1134/S1607672925601222","DOIUrl":"10.1134/S1607672925601222","url":null,"abstract":"DHX9 helicase and its ortholog MLE in D. melanogaster participate in different stages of gene expression. Both helicases are important for the formation and functioning of the nervous system in humans and D. melanogaster, respectively. However, the role of helicase activity of DHX9 and MLE in the regulation of gene expression has been poorly studied, and the existing data are quite contradictory. This work is devoted to the study of the role of helicase activity of MLE in the regulation of gene expression in D. melanogaster. A site of intense MLE binding was found on chromosome 4 of D. melanogaster, in locus 102F. It was shown that MLE is a co-activator of expression of the Dyrk3, Toy, Sox102F, Shaven, and Fuss genes located in this locus. For this, the helicase activity of MLE is required. Genes whose expression depends on MLE are expressed at a high level in the nervous system of D. melanogaster and are essential for its proper development. The obtained data contributes to the study of potentially evolutionarily conserved functions of MLE.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"524 1","pages":"509 - 513"},"PeriodicalIF":0.7,"publicationDate":"2025-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145285447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of 6-Azido-2-Chloropurine-2′-Deoxyriboside by Enzymatic Transglycosylation Reaction Catalyzed by Lactobacillus leichmannii Type II Nucleoside Deoxyribosyltransferase 利氏乳杆菌II型核苷脱氧核糖基转移酶催化的酶转糖基化反应制备6-叠氮-2-氯嘌呤-2′-脱氧核糖苷

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI: 10.1134/S160767292560040X

C. S. Alexeev, M. A. Konkina, N. N. Kurochkin, M. S. Drenichev

引用次数: 0

Effect of a Comparative Analysis of Different Annotations of the Oryza sativa Rice Genome for In Silico Verification of Predicted Promoter Sequences 水稻基因组不同注释比对分析对启动子序列预测的影响

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI: 10.1134/S1607672925600332

A. N. Bubnova, I. V. Yakovleva, A. M. Kamionskaya

引用次数: 0

Protein Complementation Approach for Quantitative and Qualitative Analysis of hTERP in Cell 细胞中hTERP定量和定性分析的蛋白质互补方法。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-21 DOI: 10.1134/S1607672925600514

M. A. Shamonova, M. S. Koriagina, V. L. Shliapina, O. A. Dontsova, M. P. Rubtsova

引用次数: 0

HDAC3 Regulates Ferroptosis via Nrf2–GPX4 Signaling in Colorectal Cancer Cells HDAC3通过Nrf2-GPX4信号通路调控结直肠癌细胞铁下垂。

IF 0.7 4区生物学

Doklady Biochemistry and Biophysics Pub Date : 2025-09-14 DOI: 10.1134/S1607672925600496

Wei Jin, Jue-jue Wang, Yan-fei Feng, Bing Chen, Zhao-hua Hu

{"title":"HDAC3 Regulates Ferroptosis via Nrf2–GPX4 Signaling in Colorectal Cancer Cells","authors":"Wei Jin, Jue-jue Wang, Yan-fei Feng, Bing Chen, Zhao-hua Hu","doi":"10.1134/S1607672925600496","DOIUrl":"10.1134/S1607672925600496","url":null,"abstract":"Ferroptosis, an iron-dependent form of regulated cell death, represents an emerging therapeutic vulnerability in colorectal cancer (CRC). However, the epigenetic mechanisms controlling ferroptosis sensitivity in CRC remain poorly understood. Here, we identify histone deacetylase 3 (HDAC3) as a pivotal epigenetic suppressor of ferroptosis. Both pharmacological inhibition and genetic knockdown of HDAC3 significantly enhanced ferroptosis sensitivity, as evidenced by elevated intracellular ferrous iron (Fe2+) and lipid peroxidation. Mechanistically, inhibition of HDAC3 reduced the expression of nuclear factor erythroid 2–related factor 2 (NRF2), a master antioxidant transcription factor, thereby leading to downregulation of glutathione peroxidase 4 (GPX4), a central ferroptosis defense gene. Notably, NRF2 knockdown abolished GPX4 downregulation by HDAC3 inhibition, whereas GPX4 overexpression rescued the ferroptotic phenotype caused by HDAC3 depletion. Collectively, these findings define an HDAC3–NRF2–GPX4 axis that suppresses ferroptosis in CRC, and highlight HDAC3 as a potential therapeutic target for ferroptosis-based cancer treatment.","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"523 1","pages":"424 - 433"},"PeriodicalIF":0.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1134/S1607672925600496.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0