Doklady Biochemistry and Biophysics最新文献

{"title":"Flowering Initiation Genes Determine the Node for Inflorence-Ear in Zea Mays L.","authors":"D. Kh. Arkhestova,&nbsp;E. Z. Kochieva,&nbsp;A. V. Shchennikova","doi":"10.1134/S1607672925600320","DOIUrl":"10.1134/S1607672925600320","url":null,"abstract":"<p>The expression profile of key genes regulating flowering initiation (<i>ID</i>1, <i>DLF</i>1, <i>ZCN</i>6, <i>ZCN</i>7, and <i>ZCN</i>8) of <i>Zea mays</i> L. was determined in the leaf axils of the reproductive and adjacent nodes during the flowering initiation. The similarity of the inter-node dynamics of the <i>ID</i>1, <i>DLF</i>1, <i>ZCN</i>7, and <i>ZCN</i>8 gene expression was shown throughout the entire measurement period. It was determined that ~10 days before visual detection of the inflorescence meristem, the expression of the flowering activator genes <i>ID</i>1, <i>DLF</i>1, <i>ZCN</i>7, and <i>ZCN</i>8 significantly increased in the reproductive node compared to the adjacent nodes, while the flowering inhibitor gene <i>ZCN</i>6 expression decreased to trace values at all nodes.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"523 1","pages":"434 - 438"},"PeriodicalIF":0.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Diurnal Dynamics of Myocardial Infarction with Distribution of Spikes in TiNi Detector Readings","authors":"G. Ts. Dambaev,&nbsp;V. Ya. Erofeev,&nbsp;A. A. Garganeeva,&nbsp;E. A. Kuzheleva,&nbsp;S. A. Okrugin","doi":"10.1134/S1607672925600423","DOIUrl":"10.1134/S1607672925600423","url":null,"abstract":"<p>A comparison of the incidence of myocardial infarction during the day was performed with the diurnal distribution of spikes in TiNi detector readings. Based on long-term monitoring data, it was shown that the maximum number of spikes on the TiNi detector graphs is observed between 7 and 10 a.m. local time. It is known that a similar diurnal pattern occurs in the case of cardiovascular complications. Based on the analysis of synchronism effects previously discovered using the TiNi detector, the properties of the factor acting on the detector, capable of influencing both the behavior of the TiNi system and the state of living organisms, were determined. This approach allows us to obtain new data on the mechanisms of the impact of external factors on the state of the biosphere.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"523 1","pages":"471 - 476"},"PeriodicalIF":0.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biphasic Effects of bFGF Blockade on Lung Cancer Cell Invasion","authors":"Jiaqi Li,&nbsp;Zhiyong Wang,&nbsp;Yanxin Lu,&nbsp;Qiang Xia,&nbsp;Pei Wei","doi":"10.1134/S1607672925600198","DOIUrl":"10.1134/S1607672925600198","url":null,"abstract":"<p>Basic fibroblast growth factor (bFGF) is a potent mitogen implicated in tumor progression, yet its precise role in cancer cell invasion remains multifaceted. Here, we unveiled a dose-dependent, biphasic regulatory effect of bFGF blockade on the invasiveness of lung cancer cells. Using a neutralizing monoclonal antibody against bFGF (bFGF Ab), we demonstrated that moderate neutralization of bFGF inhibited tumor cell invasion. Conversely, excessive bFGF blockade paradoxically enhanced the invasive capacity of lung cancer cells. Furthermore, we showed that the divergent invasive phenotypes arising from varying degrees of bFGF inhibition were mediated by altered vascular endothelial growth factor (VEGF) secretion. These findings underscore the critical importance of dose optimization in bFGF-targeted therapies, particularly concerning their impact on lung cancer invasiveness, and identify VEGF as a key mediator of these effects.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"523 1","pages":"419 - 423"},"PeriodicalIF":0.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor Effect of Helium Ions with Energy of 320 MeV/Ion during Irradiation of Ehrlich Ascites Carcinoma Cells Ex Vivo","authors":"V. E. Balakin,&nbsp;N. S. Strelnikova,&nbsp;O. M. Rozanova,&nbsp;E. N. Smirnova,&nbsp;T. A. Belyakova,&nbsp;A. E. Shemyakov","doi":"10.1134/S1607672925600356","DOIUrl":"10.1134/S1607672925600356","url":null,"abstract":"<p>The regularities of tumor induction and growth were studied in mice after a single ex vivo irradiation with a helium ion beam of Ehrlich adenocarcinoma ascites cells (EAC) at doses of 10 and 20 Gy in two positions of the Bragg curve (before the peak and at the peak) in comparison with X-ray radiation at the same doses. It was shown that the frequency of induction and delay in tumor appearance depend on the dose of helium ion irradiation. The following parameters were determined: the time of a fivefold increase in the EAC volume, tumor growth inhibition, tumor growth index (TGI), and lifetime increase (LTI) in mice. A decrease in the TGI values and an increase in the LTI values occurred with increasing dose for all types of radiation. The relative biological effectiveness (RBE) value for helium ions determined by the area under the EAC growth dynamics curves reached a maximum value of 1.8 upon irradiation at the Bragg peak at a dose of 20 Gy.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"302 - 308"},"PeriodicalIF":0.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for Functionality of Transmembrane Domains of Predicted Non-Canonical Plant Phosphotransmitters","authors":"S. N. Lomin,&nbsp;E. M. Savelieva,&nbsp;A. S. Elanskaya,&nbsp;D. V. Arkhipov,&nbsp;G. A. Romanov","doi":"10.1134/S1607672925600381","DOIUrl":"10.1134/S1607672925600381","url":null,"abstract":"<p>Bioinformatic methods have been used to predict a new subclass of proteins among plant phosphotransmitters involved in the signaling system of multistep phosphorelay. In contrast to the canonical soluble nucleocytosolic forms, the found noncanonical phosphotransmitter sequences, belonging to a wide range of plant taxa, potentially contain transmembrane domains. This suggests localization of such proteins on cell membranes and, therefore, a different function in signaling than that of canonical phosphotransmitters. We tested the functionality of the transmembrane domain of the phosphotransmitter using the protein of the tea plant <i>Camellia sinensis</i>. The membrane localization of the transiently expressed recombinant phosphotransmitter with this domain was confirmed by microscopy and immunoblotting. Thus, this is the first study to obtain experimental evidence for the existence of membrane-bound plant phosphotransmitters with as yet unknown functions. These data suggest the presence of a noncanonical membrane branch of signal transduction in the multistep phosphorelay system in plants.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"309 - 314"},"PeriodicalIF":0.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144207323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Vβ-Segment Diversity of T-cell Receptor in Techa Riverside Residents Chronically Exposed to Radiation in the Long-Term Period","authors":"A. I. Kotikova,&nbsp;E. A. Blinova,&nbsp;A. V. Akleyev","doi":"10.1134/S1607672925700164","DOIUrl":"10.1134/S1607672925700164","url":null,"abstract":"<p>To study the repertoire of the T-cell receptor in chronically exposed persons in the long-term period. The study involved 48 people who were divided into two groups: a group of exposed persons (31 individuals with the mean accumulated dose to red bone marrow (RBM) of 981 ± 130 mGy) and a comparison group (17 individuals, the mean accumulated dose to RBM of 25.3 ± 5.91 mGy). The study groups did not differ significantly in age, gender, and ethnicity. The repertoire of Vβ-segments of the T-cell receptor of the peripheral blood T-lymphocytes of exposed persons was analyzed by flow cytometry method. 24 Vβ-segments of the T-cell receptor were studied. Statistical processing of the obtained data was carried out using the Wilcoxon signed-rank test, and a direct description of Vβ-segment repertoire of the T-cell receptor was performed using the Lorenz curve and the Gini-TCR index. The study revealed a statistically significant increase in the number of Vβ3 and Vβ5.2 T-cell receptor segments in exposed individuals relative to the comparison group (<i>p</i> = 0.03 and <i>p</i> = 0.003, respectively). It was also shown that the distribution of the Vβ-segments of the T-cell receptor was uneven in both study groups. However, there was no significant difference between the repertoires of the T-cell receptor of the studied groups by the Gini-TCR index (<i>p</i> = 0.14).</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"404 - 409"},"PeriodicalIF":0.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of Nutritional and Biotechnological Value of Muscle Tissue of Mesopelagic Fishes of the North-East Atlantic Region","authors":"V. P. Voronin,&nbsp;D. V. Artemenkov,&nbsp;A. M. Orlov,&nbsp;S. A. Murzina","doi":"10.1134/S1607672925600307","DOIUrl":"10.1134/S1607672925600307","url":null,"abstract":"<p>A comparative analysis of some nutritional and biotechnological characteristics of muscle tissue of the most widespread and abundant mesopelagic fish species inhabiting the Irminger Sea (North Atlantic) was carried out. At the level of lipid metabolism, potential nutritional value was established for non-commercial <i>Notoscopelus kroyeri</i> and <i>Serrivomer beanii</i> along with the commercial beaked redfish <i>Sebastes mentella</i>. For <i>Lampanyctus macdonaldi</i> and, partially, <i>Borostomias antarcticus</i>, possible biotechnological value was shown in terms of extracting individual valuable molecules and substances of lipid nature. The other studied species (<i>Bathylagus euryops</i>, <i>Chauliodus sloani</i>, <i>Malacosteus niger</i>, <i>Scopelogadus beanii</i>, and <i>Stomias boa</i>), as well as <i>B. antarcticus</i>, are very promising for their use in aquaculture in the development of innovative feed compositions and additives for them. It was found that the depth and area of catch don’t have a significant effect on the nutritional and biotechnological characteristics of the muscle tissue of the studied species. The exception is <i>B. euryops</i>, the characteristics under consideration of which vary depending on the area of catch; for <i>S. mentella</i> and <i>S. beanii</i>, on the depth of catch. The results of the study can serve as a basis for the development and improvement of methods and protocols for sustainable use of deep-sea biological resources in the process of their development in the North Atlantic region, as well as determine the areas of practical use of these species and the format of products obtained from them or with their use (feed, additive, pure substance, composition, functional food product, etc.).</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"289 - 296"},"PeriodicalIF":0.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Damage Index and Its Dynamics in Patients with Non-Systemic Variants of Juvenile Arthritis during the Treatment with Biologics","authors":"Z. A. Kolkhidova,&nbsp;I. P. Nikishina,&nbsp;S. I. Glukhova","doi":"10.1134/S1607672925700127","DOIUrl":"10.1134/S1607672925700127","url":null,"abstract":"&lt;p&gt;Juvenile idiopathic arthritis (JIA) is characterized by a high risk of joint destruction, extra-articular changes, and rapidly progressive disability. The CHAQ (Childhood Health Assessment Questionnaire) and  X-ray are often used to assess functional impairment and the degree of irreversible damage, but both methods have limitations. The Juvenile Arthritis Damage Index (JADI) has been developed to comprehensively assess the biological prognosis of JIA, which includes articular (JADI-A) and extra-articular (JADI-E) damage evaluation. The use of the JADI may be an effective tool for predicting and monitoring the effectiveness of JIA therapy in real clinical practice conditions.&lt;/p&gt;&lt;p&gt;of the study was to identify correlations of the JADI index with features of clinical manifestations in patients with non-systemic variants of JIA who had no experience with biologic therapy (B) and to evaluate the impact of lesions defined through the JADI damage index value on predicting response to B.&lt;/p&gt;&lt;p&gt;&lt;b&gt;.&lt;/b&gt; This open prospective study included 150 children with non-systemic JIA variants with no history of biologic therapy. The mean age was 12.2 ± 4.6 years, 60% were girls; 112 patients were examined in dynamics after 6 or 12 months from the beginning of therapy with biologics. Clinical and anamnestic evaluations, including detailed assessment of joint status and JADI-A and JADI-E scores, were performed on all patients.&lt;/p&gt;&lt;p&gt;&lt;b&gt;.&lt;/b&gt; The study showed that 50% of patients had damage on the JADI score, with 43% having JADI-A+ and 23% having JADI-E+. Both articular and extra-articular damage were present in 15% (&lt;i&gt;n&lt;/i&gt; = 23) of children. Among those with JADI-A, flexion contractures of the knee joints were most frequently noted in 39%, elbow joints in 28%, and limitation of movement in the cervical spine in 26%. Extra-articular injuries were more frequently presented as avascular necrosis of bones, confirmed by imaging methods (41%), and a significant difference in limb length was observed in 35% of patients. The presence of articular changes was significantly associated with positivity for antinuclear antibody, rheumatoid factor, and anticyclic citrullinated peptide, as well as the absence of enthesitis, elevated erythrocyte sedimentation rate and С-reactive protein, high clinical activity, Juvenile Arthritis Disease Activity Score, CHAQ values, a history of glucocorticoid therapy, and low adherence to treatment. Polyarticular onset involving small hand joints and a tendency for rapid contracture formation were also associated with positive JADI-A values. The onset of polyarthritis with involvement of the small joints of the hands and a tendency to fast contracture formation were also associated with JADI-A positivity. As a result of therapy with biologics prescribed in routine clinical practice, the vast majority of patients achieved stable status without signs of damage or the value of the damage index remained unchanged. In 26 (23%) patients, JADI-A s","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"358 - 368"},"PeriodicalIF":0.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cognitive Impairments in Patients with Rheumatoid Arthritis and Comorbid Anxiety and Depressive Disorders: Outcomes of the Five-Year Prospective Study","authors":"A. A. Abramkin,&nbsp;T. A. Lisitsyna,&nbsp;D. Yu. Veltishchev,&nbsp;O. F. Seravina,&nbsp;O. B. Kovleskaya,&nbsp;S. I. Glukhova,&nbsp; E. L. Nasonov","doi":"10.1134/S1607672925700061","DOIUrl":"10.1134/S1607672925700061","url":null,"abstract":"<p>The aim of this study was to assess the baseline rates and five-years outcomes of mild cognitive impairments (MCIs) in patients with rheumatoid arthritis (RA) and comorbid anxiety and depressive disorders (ADDs) receiving traditional synthetic disease-modifying antirheumatic drugs (csDMARDs) alone or in combination with biologic DMARDs (bDMARDs) and/or adequate psychopharmacotherapy (PPT), as well as to assess the factors associated with MCI after five years.</p><p>A total of 128 RA patients were enrolled, ADDs were diagnosed in 123 (96.1%) patients by a licensed psychiatrist. Severity of depression and anxiety was evaluated with Montgomery–Asberg and Hamilton Anxiety scales. CIs were diagnosed during clinical and psychological examination using the battery of pathopsychological and projective techniques. CI outcomes were considered favourable in cases with no CI diagnosed throughout the study and in cases of CI reversal. PPT was offered, 52 (42.3%) patients agreed. Patients were  divided into the following treatment groups: сsDMARDs (<i>n</i> = 39), сsDMARDs + PPT (<i>n</i> = 43), сsDMARDs + bDMARDs (<i>n</i> = 32), and сsDMARDs + bDMARDs + PPT (<i>n</i> = 9). Multivariable logistic regression was performed to determine factors associated with CI after five years.</p><p>MCIs were diagnosed in the majority of RA patients (73.2%), including logical thinking impairments (51.2%) and memory deficit (67.5%). At a 5-year endpoint, 74 patients were included. Total CI rates in no-PPT groups increased from 69 to 85.7% (<i>p</i> = 0.024) and was higher compared to PPT groups (<i>р</i> = 0.021, 85.7% vs 62.5%, RR 1.37). Patients with favourable CI outcomes had lower major depression prevalence and baseline Montgomery–Asberg scores, major improvement in depression symptoms, and higher rates of ADD remission after five years. Baseline DAS28 (OR 1.29, <i>p</i> &lt; 0.001) was positively associated and remission of ADD negatively associated with MCI after five years (OR 0.25, <i>p</i> = 0.03), <i>R</i>² = 0.48, <i>p</i> &lt; 0.001.</p><p>ADDs and MCIs are highly prevalent in RA patients. While CIs tend to persist and worsen over time, PPT is associated with lower CI rates in long-term perspective. Personalized PPT with antidepressants and neuroleptics may show potential to lessen the rates of MCIs in RA patients with ADDs.</p>","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"323 - 332"},"PeriodicalIF":0.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thrombotic Antiphospholipid Syndrome: Recurrent Thromboses","authors":"T. M. Reshetnyak,&nbsp;F. A. Cheldieva,&nbsp;S. I. Glukhova,&nbsp;K. S. Nurbaeva,&nbsp;N. V. Seredavkina,&nbsp;M. V. Cherkasova,&nbsp;A. M. Lila,&nbsp; E. L. Nasonov","doi":"10.1134/S1607672925700152","DOIUrl":"10.1134/S1607672925700152","url":null,"abstract":"&lt;p&gt;Thrombotic antiphospholipid syndrome (APS) is a condition affecting young people in whom a thromboembolic event occurs in the presence of circulating antiphospholipid antibodies (aPL).&lt;/p&gt;&lt;p&gt; was to evaluate the incidence of recurrent thrombosis and its risk factors in antiphospholipid syndrome.&lt;/p&gt;&lt;p&gt;&lt;b&gt;.&lt;/b&gt; The retrospective study included 98 patients with APS who were followed up at the institute from 2014 to 2023, of whom 66 (67%) were women and 32 (33%) were men. Of the 98 patients with APS, 48 (49%) had a diagnosis of systemic lupus erythematosus (SLE). Antiphospholipid antibodies (aPL), including the antibodies to cardiolipin (IgG/IgM aCL), antibodies to ß2glycoprotein 1 (IgG/IgM aß2GP1), antibodies to ß2 glycoprotein IgG against domain 1 (IgG aß2GP1-D1), antibodies to phosphatidylserine/prothrombin complex (IgG/IgM aPS/PT), and other thrombotic risk factors were determined. aPL was assessed by enzyme-linked immunosorbent assay (ELISA) and chemiluminescence assay (CLA).&lt;/p&gt;&lt;p&gt;Thrombosis recurrence was reported in 62 (63%) of 98 patients, and in 36 (35%) it was not reported. The main cause of recurrent thrombosis was treatment with direct oral anticoagulants (DOACs). Twenty-four (38.7%) of  62 patients with recurrent thrombosis were treated with DOACs, the duration of which ranged from 6 to 24 months. The next most common cause of recurrent thrombosis was the lack of continuous anticoagulant therapy in 20 (32.5%) patients. In 17 (27.4%) patients, recurrence occurred while they were still taking warfarin. In 10 (41.7%) of the 24 patients, the recurrent thrombosis was arterial in origin. This was associated with recurrent cerebral circulation problems. The level of positivity for aPL did not matter, but all of them had triple IgG-aPL positivity. Five patients had lupus anticoagulant (LA) at the onset of the disease before anticoagulant therapy. IgG-aPS/PT was most important in association with recurring thrombosis in the ELISA: 45 (72.6%) of 62 patients with recurring thrombosis were positive for IgG-aPS/PT as compared with 19 (52.8%) of 36 patients without recurring thrombosis. The detection of all aPLs was more frequent in CLA than in ELISA. However, the determination of aPL by ELISA is recommended according to the latest classification criteria. Triple positivity for IgG aCL, IgG aß2GP1, and IgG aß2GP1-D1 according to CLA data remained a risk factor for recurrent thrombosis and increased the risk of recurrence more than 3 times. Obesity was a risk factor for recurrent thrombosis, with a 5-fold increased risk of recurrent thrombosis in obese patients compared to the non-obese ones (&lt;i&gt;p&lt;/i&gt; = 0.01).&lt;/p&gt;&lt;p&gt;&lt;b&gt;.&lt;/b&gt; Recurrent thrombosis in APS is largely associated with IgG aCL, IgG aß2GP1, IgG aß2GP1-D1, and IgG aPS/PT. Triple IgG aPL positivity in any combination significantly increased recurrent thrombosis risk. The presence of any type of IgG aPL in both ELISA and CLA influenced the recurrence rate of thrombosis in APS. Obesity was a ","PeriodicalId":529,"journal":{"name":"Doklady Biochemistry and Biophysics","volume":"522 1","pages":"375 - 386"},"PeriodicalIF":0.7,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}