Chemoprotective Effect of Myrrhone against Diethylnitrosamine and Ferric Nitrile Induced Renal Cancer via Alteration of HO-1/Nrf2 and TRL4/NF-κB Signaling Pathway.

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Qian Yu, Ling Tian, Jiwei Zhang
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引用次数: 0

Abstract

. Renal cell carcinoma (RCC) is the most prevalent form of kidney cancer and is the primary malignancy affecting the genitourinary system. It represents the majority of kidney cancer cases and is distinguished by its aggressive nature and high mortality rate. The current study investigates the chemoprotective effect of myrrhone against Diethylnitrosamine (DEN) and ferric nitrile (Fe-NTA) induced RCC in rats and elucidates the underlying mechanism.

Methods: . Following a single dose of intraperitoneal DEN (200 mg/kg) and a twice-weekly administration of Fe-NTA, rats were administered either an oral dose of myrrhone (5, 10, or 15 mg/kg). The body weights and food intake of the rats were monitored at regular intervals, and the levels of renal cancer markers, antioxidants, inflammatory markers, and other parameters were assessed. Additionally, histopathological studies were conducted on the renal tissues, and the mRNA expression of Bax, Bcl-2, HO-1, SOD2, mtDNA, ATP8, PGC-1α, TRL4, and NF-κB was analyzed.

Results: . The dosage-dependent administration of myrrhone demonstrated a remarkable suppression of tumor incidence and an improvement in body weight and food intake. Myrrhone markedly decreased the level of ODC, Thymidine [3H] incorporation, and renal parameters such as creatinine, uric acid, BUN, Kim-1, Cysc-C, and LDH. Additionally, myrrhone significantly altered the levels of MDA, GSH, GPx, CAT, and SOD, as well as inflammatory cytokines such as TNF-α, INF-γ, IL-1β, IL-6, and IL-10, and inflammatory parameters such as COX-2, PGE2, TGF-β1, NF-κB, and iNOS. Furthermore, myrrhone significantly decreased the histopathological score and improved the condition of histopathology. Finally, myrrhone significantly altered the mRNA expression of Bax, Bcl-2, HO-1, SOD2, mtDNA, ATP8, PGC-1α, TRL4, and NF-κB.

Conclusion: : The result clearly showed the chemoprotective effect of myrrhone against diethylnitrosamine and ferric nitrile induced Renal Cancer via alteration of HO-1/Nrf2 and TRL4/NF-κB Signaling pathway.

没药酮通过改变HO-1/Nrf2和TRL4/NF-κB信号通路对二乙基亚硝胺和三丁腈诱导的肾癌的化学保护作用
. 肾细胞癌(RCC)是肾癌最常见的形式,是影响泌尿生殖系统的原发性恶性肿瘤。它代表了大多数肾癌病例,其特点是其侵袭性和高死亡率。本研究探讨没药酮对二乙基亚硝胺(DEN)和三丁腈铁(Fe-NTA)诱导的大鼠肾细胞癌的化学保护作用,并探讨其机制。方法:。在单次腹腔注射DEN (200 mg/kg)和每周两次给药Fe-NTA后,给大鼠口服没药酮(5、10或15 mg/kg)。定期监测大鼠的体重和食物摄入量,并评估肾癌标志物、抗氧化剂、炎症标志物等指标的水平。并对大鼠肾组织进行组织病理学研究,分析Bax、Bcl-2、HO-1、SOD2、mtDNA、ATP8、PGC-1α、TRL4、NF-κB mRNA表达情况。结果:。没药酮的剂量依赖性管理显示出肿瘤发生率的显著抑制和体重和食物摄入量的改善。没药酮显著降低ODC、胸苷[3H]掺入水平和肾参数,如肌酐、尿酸、BUN、Kim-1、Cysc-C和LDH。此外,没药酮显著改变MDA、GSH、GPx、CAT、SOD水平,以及炎症因子TNF-α、INF-γ、IL-1β、IL-6、IL-10水平,炎症参数COX-2、PGE2、TGF-β1、NF-κB、iNOS水平。此外,没药酮显著降低组织病理评分,改善组织病理状况。最后,没药酮显著改变Bax、Bcl-2、HO-1、SOD2、mtDNA、ATP8、PGC-1α、TRL4和NF-κB的mRNA表达。结论:没药酮通过改变HO-1/Nrf2和TRL4/NF-κB信号通路,对二乙基亚硝胺和铁腈诱导的肾癌具有化学保护作用。
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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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