果蝇mod(mdg4)基因第4内含子5′端保守序列在反式剪接中的功能研究

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Iu. V. Soldatova, O. Beginyazova,  P. G. Georgiev, M. V. Tikhonov
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引用次数: 0

摘要

选择性剪接是提供蛋白质遗传多样性的重要机制。在黑腹果蝇中发现了独特的基因座,其中mRNA多样性是反式剪接的结果,这是一种将不同前mRNA的外显子连接在一起的过程。mod(mdg4)基因座编码超过31种同种异构体,对其反式剪接进行了详细的研究。这个过程的重要元素包括前面描述的第四个内含子的保守序列。本研究的目的是进一步表征第四个内含子的保守基序,特别是内含子5'端的元素。利用转基因模型株系,研究表明所研究元件序列的改变会导致反式剪接的中断。相比之下,内源性基因座的类似变化不会导致反式剪接的中断。因此,保守元件在反式剪接中起作用,但不是关键。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of the Functional Role of the Conserved Sequence at the 5'-End of the Fourth Intron of the mod(mdg4) Gene in Trans-Splicing in Drosophila melanogaster

Alternative splicing is an important mechanism that provides genetic diversity of proteins. Unique loci have been identified in Drosophila melanogaster, where mRNA diversity arises as a result of trans-splicing—a process in which exons from different pre-mRNAs are joined together. The trans-splicing in the mod(mdg4) locus, which encodes more than 31 isoforms, has been studied in detail. Important elements for this process include previously described conserved sequences in the fourth intron. The aim of this study is to further characterize the conserved motifs of the fourth intron, specifically the element at the 5'-end of the intron. Using model transgenic lines, it has been shown that introduced changes in the sequence of the studied element lead to a disruption of trans-splicing. In contrast, similar changes in the endogenous locus did not result in a disruption of trans-splicing. Thus, the conserved element plays a role in trans-splicing but is not critical.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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