黑腹果蝇Cp190蛋白功能高度保守区域的鉴定

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-04-11 DOI:10.1134/S1607672924601434

L. S. Melnikova, K. I. Balagurov, P. G. Georgiev, A. K. Golovnin

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引用次数: 0

摘要

CP190蛋白结合家政基因启动子和绝缘子/边界元件，并在其活性中起关键作用。这项工作的目的是研究CP190蛋白高度保守区域的缺失对其功能的影响。结果表明，664 ~ 700aa序列的缺失会导致致死性表型。因此，在CP190中发现了一个新的区域，该区域在CP190蛋白与尚未确定的伴侣蛋白的相互作用，CP190形成的蛋白复合物的稳定以及它们在染色质上的募集中发挥重要作用。

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Identification of a Highly Conserved Region Critical for the Functionality of the Cp190 Protein in Drosophila melanogaster

The CP190 protein binds to both housekeeping gene promoters and insulator/boundary elements and plays a critical role in their activity. The aim of this work was to study the effect of deletions in highly conserved regions of the CP190 protein on its functionality. It was shown that deletion of the sequence from 664 to 700 aa leads to a lethal phenotype. Thus, a new region was identified in CP190 that plays an important role in the interaction of the CP190 protein with as yet unidentified partner proteins, stabilization of protein complexes formed by CP190, and their recruitment to chromatin.

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.