Functional Role of Bap170 Domains in Enhancer-Dependent Gene Activity in Drosophila melanogaster.

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-01-31 DOI:10.1134/S1607672924601409

V K Chmykhalo, D Amendola, Y V Shidlovskii, L A Lebedeva, P Schedl, E Giordano

引用次数: 0

Abstract

The Bap170 subunit of the SWI/SNF chromatin remodeler exhibits activator functions when artificially recruited to the LacZ reporter promoter in enhancer-dependent transcription. In this study, the functional significance of Bap170 protein domains in reporter activation was analyzed. Deletion of the ARID domain does not reduce Bap170 activity. Increased expression of the LacZ reporter was observed in the form of Bap170 without a region that includes LXXLL motifs. Deletions of the central (RFX domain and IDRs) and C-terminal region (zinc fingers) of Bap170 lead to a significant decrease in transgene expression. Apparently, these regions of Bap170 are critical for the function of this protein in enhancer-dependent transcription.

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Bap170结构域在果蝇增强子依赖基因活性中的功能作用。

SWI/SNF染色质重塑子的Bap170亚基在增强子依赖性转录中被人工招募到LacZ报告子上时表现出激活子功能。本研究分析了Bap170蛋白结构域在报告基因激活中的功能意义。删除ARID结构域不会降低Bap170的活性。LacZ报告基因以Bap170的形式表达增加，不含LXXLL基序的区域。Bap170的中心区域（RFX结构域和IDRs）和c端区域（锌指）缺失导致转基因表达显著降低。显然，Bap170的这些区域对该蛋白在增强子依赖性转录中的功能至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.