黑腹果蝇双胸复合体bxd PRE具有弱绝缘体活性

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-04-11 DOI:10.1134/S1607672924601513

A. N. Ibragimov, O. V. Kyrchanova, Y. E. Vorontsova, E. N. Kozlov, V. O. Dubrovskaya, P. G. Georgiev

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引用次数: 0

摘要

果蝇Bithorax复合体（BX-C）中调控域的自主功能是由边界（绝缘子）维持的，该边界（绝缘子）防止不适当的增强子-启动子相互作用。多梳响应元件（Polycomb response elements, PREs）在调控域内维持表观遗传记忆，通常位于绝缘子附近，增强了它们的阻断活性。bxd PRE是一种已知的沉默基因，可调控第一腹肌段Ubx基因的bxd/pbx结构域。我们使用边界替换策略来评估bxd PRE的绝缘子活性。用bxd PRE取代BX-C中分离Abd-B调控结构域的Fab-7边界，发现bxd PRE具有较弱的绝缘子活性。

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The bxd PRE from Bithorax Complex of Drosophila melanogaster Has Weak Insulator Activity

The autonomous functioning of regulatory domains in the Bithorax complex (BX-C) of Drosophila melanogaster is maintained by boundaries (insulators) that prevent inappropriate enhancer–promoter interactions. Polycomb response elements (PREs) maintain epigenetic memory within regulatory domains and are often located near insulators, enhancing their blocking activity. The bxd PRE is a well-characterized silencer that regulates the bxd/pbx domain of the Ubx gene in the first abdominal segment. We used a boundary replacement strategy to assess the insulator activity of the bxd PRE. Substituting the bxd PRE for the Fab-7 boundary, which separates the Abd-B regulatory domains in the BX-C, revealed that the bxd PRE has weak insulator activity.

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.