Avicularin作为妊娠期糖尿病的一种有前景的治疗药物：炎症、氧化应激和凋亡通路的调节

IF 0.8 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Doklady Biochemistry and Biophysics Pub Date : 2025-04-11 DOI:10.1134/S1607672924601471

Fang Wang, Yakun Zhao, Jiejie Xing, Lingling Gong

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Blood glucose levels (BGL) and plasma insulin were assessed at various time intervals. Levels of glycogen, glycated hemoglobin (HbA1c), hemoglobin (Hb), visfatin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), antioxidants, lipids, inflammatory cytokines, apoptotic markers, and inflammatory parameters were evaluated. Additionally, mRNA expression was analyzed in hepatic tissue.Results. Avicularin significantly (p < 0.001) improved the body weight, fetal body weight and reduced the placental weight. Avicularin significantly (p < 0.001) suppressed the elevated blood glucose level and improved the plasma insulin level. Avicularin significantly (p < 0.001) altered the levels of HbA1c, Hb, visfatin, ICAM-1, VCAM-1, LPO, CAT, GSH, GPx, SOD, GST, TNF-α, IL-1β, IL-6, Il-8, IL-10, IL-18, COX-2, PGE2, iNOS, NF-κB, Bax, Bcl-2, Bcl-2:Bax ratio and caspase-3 parameters. 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Levels of glycogen, glycated hemoglobin (HbA1c), hemoglobin (Hb), visfatin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), antioxidants, lipids, inflammatory cytokines, apoptotic markers, and inflammatory parameters were evaluated. Additionally, mRNA expression was analyzed in hepatic tissue.Results. Avicularin significantly (p < 0.001) improved the body weight, fetal body weight and reduced the placental weight. Avicularin significantly (p < 0.001) suppressed the elevated blood glucose level and improved the plasma insulin level. Avicularin significantly (p < 0.001) altered the levels of HbA1c, Hb, visfatin, ICAM-1, VCAM-1, LPO, CAT, GSH, GPx, SOD, GST, TNF-α, IL-1β, IL-6, Il-8, IL-10, IL-18, COX-2, PGE2, iNOS, NF-κB, Bax, Bcl-2, Bcl-2:Bax ratio and caspase-3 parameters. Avicularin treatment significantly (p < 0.001) suppressed the mRNA expression of TRL4, MyD88, NF-κB, NLRP3, RAGE, VCAM-1, MCP-1, VEGF, Nox2, and p65.Conclusion. 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引用次数: 0

摘要

背景。妊娠期糖尿病（GDM）是一种以血糖水平升高为特征的重要妊娠并发症。这种情况是由妊娠期胰岛素抵抗和葡萄糖代谢受损引起的。本实验研究了阿维霉素对链脲佐菌素（STZ）诱导的大鼠GDM的保护作用，并探讨其机制。材料和方法。本研究采用雌性瑞士Wistar大鼠。用链脲佐菌素（STZ） 25 mg/kg诱导大鼠妊娠期糖尿病（GDM）。大鼠分别以5、10、15 mg/kg剂量口服阿维霉素。测量体重、胎盘重和胎儿重。在不同的时间间隔评估血糖水平（BGL）和血浆胰岛素。评估糖原、糖化血红蛋白（HbA1c）、血红蛋白（Hb）、脂肪素、细胞间粘附分子-1 （ICAM-1）、血管细胞粘附分子-1 （VCAM-1）、抗氧化剂、脂质、炎症细胞因子、凋亡标志物和炎症参数的水平。分析肝组织中mRNA的表达。Avicularin显著(p <；0.001)改善了体重、胎儿体重，降低了胎盘重量。Avicularin显著(p <；0.001)抑制升高的血糖水平，改善血浆胰岛素水平。Avicularin显著(p <；0.001)改变了HbA1c、Hb、visfatin、ICAM-1、VCAM-1、LPO、CAT、GSH、GPx、SOD、GST、TNF-α、IL-1β、IL-6、Il-8、IL-10、IL-18、COX-2、PGE2、iNOS、NF-κB、Bax、Bcl-2、Bcl-2:Bax比值和caspase-3参数的水平。avululin治疗显著(p <；0.001)抑制TRL4、MyD88、NF-κB、NLRP3、RAGE、VCAM-1、MCP-1、VEGF、Nox2、p65的mRNA表达。本研究结果表明，阿维霉素通过调节TLR4/MyD88/NF-κB和Bax/Bcl-2/Caspase通路，对链脲佐菌素诱导的妊娠期糖尿病具有保护作用。

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Avicularin as a Promising Therapeutic Agent for Gestational Diabetes Mellitus: Modulation of Inflammatory, Oxidative Stress, and Apoptotic Pathways

ackground. Gestational diabetes mellitus (GDM) is a significant pregnancy complication characterized by elevated blood glucose levels. This condition arises from insulin resistance and impaired glucose metabolism during the gestational period. In this experimental study, we investigated the protective effect of avicularin against streptozotocin (STZ)-induced GDM in rats and explored the underlying mechanism.

Material and methods. Female Swiss Wistar rats were utilized in this study. Gestational diabetes mellitus (GDM) was induced in the rats through the administration of 25 mg/kg streptozotocin (STZ). The rats received oral administration of avicularin at doses of 5, 10, and 15 mg/kg. Body, placental, and fetal weights were measured. Blood glucose levels (BGL) and plasma insulin were assessed at various time intervals. Levels of glycogen, glycated hemoglobin (HbA1c), hemoglobin (Hb), visfatin, intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), antioxidants, lipids, inflammatory cytokines, apoptotic markers, and inflammatory parameters were evaluated. Additionally, mRNA expression was analyzed in hepatic tissue.

Results. Avicularin significantly (p < 0.001) improved the body weight, fetal body weight and reduced the placental weight. Avicularin significantly (p < 0.001) suppressed the elevated blood glucose level and improved the plasma insulin level. Avicularin significantly (p < 0.001) altered the levels of HbA1c, Hb, visfatin, ICAM-1, VCAM-1, LPO, CAT, GSH, GPx, SOD, GST, TNF-α, IL-1β, IL-6, Il-8, IL-10, IL-18, COX-2, PGE2, iNOS, NF-κB, Bax, Bcl-2, Bcl-2:Bax ratio and caspase-3 parameters. Avicularin treatment significantly (p < 0.001) suppressed the mRNA expression of TRL4, MyD88, NF-κB, NLRP3, RAGE, VCAM-1, MCP-1, VEGF, Nox2, and p65.

Conclusion. The results of this study demonstrated the protective effect of avicularin against streptozotocin-induced gestational diabetes mellitus via modulation of the TLR4/MyD88/NF-κB and Bax/Bcl-2/Caspase pathways.

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来源期刊

Doklady Biochemistry and Biophysics 生物-生化与分子生物学

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.