人蛋白SLURP-2在皮肤角质形成细胞分泌的细胞外囊泡库中的变化

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
T. Y. Gornostaeva, O. V. Shlepova, I. D. Kukushkin, A. S. Paramonov,  M. P. Kirpichnikov, E. N. Lyukmanova
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引用次数: 0

摘要

角质形成细胞分泌的细胞外囊泡参与细胞间相互作用,含有多种蛋白质、mRNA和miRNA、脂质,因此它们可以激活细胞迁移和增殖。分泌的人蛋白SLURP-2调节上皮细胞的生长和分化,影响角质形成细胞的增殖和迁移。在这项工作中,我们发现在SLURP-2存在的情况下,角质形成细胞分泌的细胞外囊泡刺激HaCaT角质形成细胞的迁移。我们还发现,在SLURP-2存在的情况下,角质形成细胞分泌的囊泡中抑制细胞迁移和增殖的miRNA-96和miRNA-183的表达降低。因此,研究表明,SLURP-2对角质形成细胞迁移的刺激,特别是与这些细胞分泌的细胞外囊泡的变化有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Changes in the Repertoire of Extracellular Vesicles Secreted by Skin Keratinocytes by the Human Protein SLURP-2

Extracellular vesicles secreted by keratinocytes are involved in intercellular interactions and contain various proteins, mRNA and miRNA, lipids, due to which they can activate cell migration and proliferation. The secreted human protein SLURP-2 regulates growth and differentiation of epithelial cells and affects proliferation and migration of keratinocytes. In this work, we showed that extracellular vesicles secreted by keratinocytes in the presence of SLURP-2 stimulate migration of HaCaT keratinocytes. It was also found that the expression of miRNA-96 and miRNA-183, suppressing cell migration and proliferation, is decreased in the vesicles secreted by keratinocytes in presence of SLURP-2. Thus, it is shown that the stimulation of keratinocyte migration in presence of SLURP-2 is associated, in particular, with a change in the repertoire of extracellular vesicles secreted by these cells.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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