一种锚定双特异性纳米抗体的研制以提高抗原在聚苯乙烯板孔中的固定化和检测效率

IF 0.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
S. V. Tillib, M. V. Panasyuk, O. S. Goryaynova, T. I. Ivanova
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引用次数: 0

摘要

在聚苯乙烯微孔板孔中进行免疫测定(IA)方法是诊断研究的基础。在“夹心”免疫接种中,最重要的初始阶段是将锚定抗体固定在板孔中,这是为特定结合来自生物液的特定抗原而设计的。一个非常有前途的抗原识别分子的选择是单域抗体(纳米抗体,Nbs)。Nbs作为锚定抗体的使用受到其在板孔中被动吸附后的低效率的阻碍。在国家统计局的情况下,开发一种新的形式和固定方法对于克服这一问题至关重要。这项工作描述了一种新型锚定双特异性纳米抗体(anchor- nb)的开发,以提高锚定- nb的被动吸附效率以及随后在聚苯乙烯板孔中固定和检测目标抗原的阶段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of an Anchor Bispecific Nanoantibody to Improve the Efficiency of Antigen Immobilization and Detection in a Well of a Polystyrene Plate

Immunoassay (IA) methods performed in the wells of a polystyrene microplate are the basis of diagnostic studies. In the “sandwich” IA, a fundamentally important initial stage is the immobilization of anchor antibodies in the well of the plate, designed for specific binding of a given antigen from a biological fluid. One of the very promising options for antigen-recognizing molecules are single-domain antibodies (nanoantibodies, Nbs). The use of Nbs as anchor antibodies is hampered by their low efficiency of functioning after passive adsorption in the well of the plate. The development of a new format and immobilization method in the case of Nbs are fundamentally important for overcoming this problem. This work describes the development of a new format of an anchor bispecific nanoantibody (anchor-Nb) to improve the efficiency of both passive adsorption of anchor-Nb and subsequent stages of immobilization and detection of the target antigen in the well of a polystyrene plate.

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来源期刊
Doklady Biochemistry and Biophysics
Doklady Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
1.60
自引率
12.50%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.
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