S. V. Tillib, M. V. Panasyuk, O. S. Goryaynova, T. I. Ivanova
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Development of an Anchor Bispecific Nanoantibody to Improve the Efficiency of Antigen Immobilization and Detection in a Well of a Polystyrene Plate
Immunoassay (IA) methods performed in the wells of a polystyrene microplate are the basis of diagnostic studies. In the “sandwich” IA, a fundamentally important initial stage is the immobilization of anchor antibodies in the well of the plate, designed for specific binding of a given antigen from a biological fluid. One of the very promising options for antigen-recognizing molecules are single-domain antibodies (nanoantibodies, Nbs). The use of Nbs as anchor antibodies is hampered by their low efficiency of functioning after passive adsorption in the well of the plate. The development of a new format and immobilization method in the case of Nbs are fundamentally important for overcoming this problem. This work describes the development of a new format of an anchor bispecific nanoantibody (anchor-Nb) to improve the efficiency of both passive adsorption of anchor-Nb and subsequent stages of immobilization and detection of the target antigen in the well of a polystyrene plate.
期刊介绍:
Doklady Biochemistry and Biophysics is a journal consisting of English translations of articles published in Russian in biochemistry and biophysics sections of the Russian-language journal Doklady Akademii Nauk. The journal''s goal is to publish the most significant new research in biochemistry and biophysics carried out in Russia today or in collaboration with Russian authors. The journal accepts only articles in the Russian language that are submitted or recommended by acting Russian or foreign members of the Russian Academy of Sciences. The journal does not accept direct submissions in English.