iScience最新文献_第8页

Integrative proteomics defines FANCA as a multifunctional hub linking DNA repair to translation. 整合蛋白质组学将FANCA定义为连接DNA修复和翻译的多功能枢纽。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117461

Virgile Gibert, Frédérique Maczkowiak-Chartois, Benedetta Mancini, Emilie-Fleur Gautier, Johanna Bruce, Morgane Le Gall, Serge Urbach, Angelos Constantinou, Anne Helbling-Leclerc, Jihane Basbous, Filippo Rosselli

{"title":"Integrative proteomics defines FANCA as a multifunctional hub linking DNA repair to translation.","authors":"Virgile Gibert, Frédérique Maczkowiak-Chartois, Benedetta Mancini, Emilie-Fleur Gautier, Johanna Bruce, Morgane Le Gall, Serge Urbach, Angelos Constantinou, Anne Helbling-Leclerc, Jihane Basbous, Filippo Rosselli","doi":"10.1016/j.isci.2026.117461","DOIUrl":"10.1016/j.isci.2026.117461","url":null,"abstract":"Fanconi anemia (FA) is a genome instability syndrome caused by defects in the FANC/BRCA DNA repair pathway, yet its broad clinical heterogeneity suggests functions beyond DNA repair. Using an integrative interactomics strategy combining endogenous co-immunoprecipitation, proximity-dependent labeling, in silico network integration, and functional analyses, we construct an interaction score-based multilayered FANCA protein-protein interaction landscape. Beyond its association with the FANCcore complex and DNA repair machineries, FANCA engages with proteins involved in chromatin remodeling, RNA metabolism, and ribosome biogenesis. Functional analyses reveal ribosome-related processes as a robust feature of FANCA-associated networks. Quantitative analyses further reveal alterations in ribosome protein stoichiometry, suggesting defective ribosome biogenesis. Our findings suggest translation alterations may explain the pleiotropic clinical manifestations of FA by posing FANCA as a link between genome maintenance, ribosome biogenesis, and translation. Our study highlights how integrative interaction allows deciphering regulatory roles of DNA repair proteins in cellular homeostasis and disease.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117461"},"PeriodicalIF":4.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13581985/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional outcomes and biochemical control after radical prostatectomy versus irreversible electroporation for localized prostate cancer. 根治性前列腺切除术与不可逆电穿孔治疗局限性前列腺癌后的功能结局和生化控制。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117303

Zhi-Yu Xia, Jia-Cheng Xiang, Yu-Xuan Yang, Fan Xiao, Jun Yang, Shao-Gang Wang, Qi-Dong Xia

{"title":"Functional outcomes and biochemical control after radical prostatectomy versus irreversible electroporation for localized prostate cancer.","authors":"Zhi-Yu Xia, Jia-Cheng Xiang, Yu-Xuan Yang, Fan Xiao, Jun Yang, Shao-Gang Wang, Qi-Dong Xia","doi":"10.1016/j.isci.2026.117303","DOIUrl":"10.1016/j.isci.2026.117303","url":null,"abstract":"Treatment selection for localized prostate cancer requires balancing oncologic control against urinary and sexual function. We retrospectively compared 32 men undergoing laparoscopic bilateral nerve-sparing radical prostatectomy (RP) with 18 undergoing focal irreversible electroporation (IRE), using unweighted and inverse probability of treatment weighting analyses. IRE was associated with shorter operative time and less frequent early urinary incontinence and sexual function decline. At 12 months after weighting, urinary incontinence occurred in 6.0% after RP and 0% after IRE, while sexual function decline occurred in 40.0% and 4.1%, respectively. Biochemical recurrence was more frequent after IRE than RP at 12 months (28.6% versus 8.0%). These findings suggest a trade-off between early functional preservation and biochemical control and should be interpreted as hypothesis-generating because of the retrospective single-center design, small sample, non-equivalent recurrence definitions, and short follow-up.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117303"},"PeriodicalIF":4.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13581978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut microbiome dysbiosis is associated with aldosterone overproduction in idiopathic hyperaldosteronism. 在特发性醛固酮增多症中，肠道微生物群失调与醛固酮过量产生有关。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117426

Yuanliang Jiang, Qinglian Zeng, Linqiang Ma, Yifan He, Shuangshuang Zhu, Yunjie Xiong, Ying Jing, Qi Zhang, Shuangxin Qi, Jinbo Hu, Qifu Li, Shumin Yang

{"title":"Gut microbiome dysbiosis is associated with aldosterone overproduction in idiopathic hyperaldosteronism.","authors":"Yuanliang Jiang, Qinglian Zeng, Linqiang Ma, Yifan He, Shuangshuang Zhu, Yunjie Xiong, Ying Jing, Qi Zhang, Shuangxin Qi, Jinbo Hu, Qifu Li, Shumin Yang","doi":"10.1016/j.isci.2026.117426","DOIUrl":"10.1016/j.isci.2026.117426","url":null,"abstract":"Idiopathic hyperaldosteronism (IHA) is a significant cause of secondary hypertension. Emerging evidence links the gut microbiota to endocrine and cardiovascular diseases. We integrated human genetics, multi-omics, and fecal microbiota transplantation (FMT) to investigate the association between IHA and gut microbiome. Two-sample Mendelian randomization identified seven bacterial taxa associated with IHA. Metagenomic analysis of 30 patients with IHA and 30 healthy control subjects revealed lower microbial diversity and depletion of protective genera identified by Mendelian randomization. Plasma metabolomics showed alterations in tryptophan metabolism and other metabolic pathways that overlapped with microbial functional changes. Tryptophan-derived metabolites correlated with the abundance of protective genera. FMT from patients with IHA increased serum aldosterone and elevated 5-hydroxy-L-tryptophan in germ-free mice. These findings support an association between gut microbiome dysbiosis, metabolic alterations, and aldosterone dysregulation, highlighting the gut microbiome as a potential target for the diagnosis and treatment of IHA.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117426"},"PeriodicalIF":4.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148966013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MYZAP promotes angiogenesis by targeting RND1 after myocardial infarction. MYZAP在心肌梗死后通过靶向RND1促进血管生成。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117423

Lina Xuan, Guangze Wang, Xiufang Li, Shengjie Wang, Yuman Gao, Jingheng Wang, Hui Zhang, Shuo Zhuang, Huishan Luo, Jianjun Guo, Mingyu Zhang, Xingmei Yang, Hailong Zhang, Qingqing Zhang, Yiyang Qiao, Kai Kang, Ying Zhang, Lihua Sun

{"title":"MYZAP promotes angiogenesis by targeting RND1 after myocardial infarction.","authors":"Lina Xuan, Guangze Wang, Xiufang Li, Shengjie Wang, Yuman Gao, Jingheng Wang, Hui Zhang, Shuo Zhuang, Huishan Luo, Jianjun Guo, Mingyu Zhang, Xingmei Yang, Hailong Zhang, Qingqing Zhang, Yiyang Qiao, Kai Kang, Ying Zhang, Lihua Sun","doi":"10.1016/j.isci.2026.117423","DOIUrl":"10.1016/j.isci.2026.117423","url":null,"abstract":"Myocardial infarction (MI) remains the leading cause of death due to cardiovascular disease worldwide. role of MYZAP in angiogenesis remains unclear. The expression of MYZAP in MI was significantly decreased. Endothelial-specific overexpression of MYZAP improved cardiac function, increased angiogenesis, and restored hindlimb blood flow levels in MI mice detected by MoorFLPIReview. The proliferation ability, migration, and invasion of endothelial cells were reduced after hypoxia, which were restored after overexpression of MYZAP or knockdown of RND1. The pro-angiogenic effect of MYZAP overexpression was reversed by RND1 overexpression. The proliferation ability, migration and invasion ability, and tube formation ability of endothelial cells were significantly reduced after knockdown of MYZAP. Direct interaction between MYZAP and RND1 was confirmed by co-immunoprecipitation (Co-IP) and thermal stability assays. TRIM21 was identified as a potential E3 ubiquitin ligase responsible for RND1 degradation. Our work revealed that endothelial-specific overexpression of MYZAP enhances angiogenesis by activating the VEGF/PI3K/AKT pathway in MI.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117423"},"PeriodicalIF":4.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13581988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geothermal heat pumps and thermal energy networks: Technical feasibility and market adoption. 地源热泵和热能网络：技术可行性和市场采用。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117432

Juliet G Simpson, Rebecca Barney, Ashreeta Prasanna, Shashwat Sharma, Ashok Sekar, Paritosh Das

引用次数: 0

Transcriptomics reveals metabolic division of labor and reproduction-metabolism decoupling in Saccharomyces cerevisiae biofilms. 转录组学揭示了酿酒酵母生物膜的代谢分工和生殖代谢解耦。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117407

Caice Liang, Jing Ruan, Biao Yang, Wei Liu, Qingguo Liu, Chaowei Zhou, Junqiang Shan, Shuqi Shi, Hui Han, Wenjun Sun, Yong Chen

引用次数: 0

Volume-inflation registration (INFREG) for morphometric analysis of human focal cortical dysplasia type II. 体积膨胀登记（INFREG）用于人类局灶性皮质发育不良II型的形态计量学分析。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117446

Yongsheng Zhang, Haojie Han, Fang Chen, Hongen Liao

{"title":"Volume-inflation registration (INFREG) for morphometric analysis of human focal cortical dysplasia type II.","authors":"Yongsheng Zhang, Haojie Han, Fang Chen, Hongen Liao","doi":"10.1016/j.isci.2026.117446","DOIUrl":"10.1016/j.isci.2026.117446","url":null,"abstract":"Neurodevelopmental expansion of human association cortex increases cortical folding complexity, causing registration inaccuracies that confound detection of subtle pathological signatures like focal cortical dysplasia (FCD) in voxel-based morphometry. We introduce INFREG (volume-inflation registration), integrating surface-based deformation with volumetric alignment to minimize sulcal smoothing. Validated on 80 histologically confirmed FCD cases and 85 controls, INFREG outperformed conventional methods (CAT12/Demons/BrainSuite) by (i) superior template to subject alignment (SSIM = 0.95 ± 0.04; GM/WM Dice = 0.93 ± 0.05 vs. 0.70-0.87, all p < 0.001); (ii) sharper templates with 23%-41% lower intensity SD and finer gyral alignment (3.34 ± 0.13 vs. 3.45 ± 0.15 mm, p < 0.01); (iii) reducing false positive FCD Type II clusters by 70%-88% (p < 0.001) in association cortices; and (iv) halving manual verification time (Observer1:101 ± 67 vs.177 ± 114 s; Observer2:75 ± 48 vs.162 ± 102 s; p < 0.001). In 30 typical cases, 90% localized within top 5 clusters, and all 12 VBM-indeterminate cases resolved. INFREG may enhance clinical workflows for FCD type II evaluation.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117446"},"PeriodicalIF":4.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13582014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural correlates of information gain during search revealed by fMRI. 功能磁共振成像显示搜索过程中信息获取的神经关联。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-09 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117445

Sakrapee Paisalnan, Yashar Moshfeghi, Frank Pollick

{"title":"Neural correlates of information gain during search revealed by fMRI.","authors":"Sakrapee Paisalnan, Yashar Moshfeghi, Frank Pollick","doi":"10.1016/j.isci.2026.117445","DOIUrl":"10.1016/j.isci.2026.117445","url":null,"abstract":"When confronted with uncertainty, humans engage in information-seeking behavior to gather sufficient information. Identifying the brain regions involved offers insight into the underlying cognitive mechanisms. Using Shannon information theory, we quantified the information contained in the image tiles presented in an fMRI experiment. Nineteen participants judged whether a caption described an image by viewing a sequence of tiles and signaling when it matched. Random-effects ANOVA and representational similarity analysis revealed that regions associated with information gain resulted in two complementary patterns. The first, involving right Ph2 and left hIP5, showed a graded relationship with information gain, consistent with tracking currently available perceptual evidence. The second, involving bilateral PGp with left hIP3 and left FG2, was more sensitive to intermediate information gain, possibly reflecting integrative processes relating accumulated visual information to task-relevant semantic expectations. These findings offer a neurocognitive framework for how information is acquired and evaluated during a sequential search.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117445"},"PeriodicalIF":4.5,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13581990/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148987489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatibility and antibacterial properties of ordered PLA fiber membranes fabricated via electrospinning technology. 静电纺丝法制备聚乳酸有序纤维膜的生物相容性和抗菌性能。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-08 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117353

Donghe Xue, Zhen Zhang, Zeyu Zhang, Xiaohu Zheng, Jialong Xu, Yao Chen, Zuo Liu, Junwen Gu, Hao Lu, Kun Li, Longwei Lu

{"title":"Biocompatibility and antibacterial properties of ordered PLA fiber membranes fabricated via electrospinning technology.","authors":"Donghe Xue, Zhen Zhang, Zeyu Zhang, Xiaohu Zheng, Jialong Xu, Yao Chen, Zuo Liu, Junwen Gu, Hao Lu, Kun Li, Longwei Lu","doi":"10.1016/j.isci.2026.117353","DOIUrl":"10.1016/j.isci.2026.117353","url":null,"abstract":"Peripheral nerve injury remains a major clinical challenge owing to its limited self-regeneration capacity and high risk of postoperative infection. In this study, highly ordered poly(lactic acid) (PLA) nanofiber membranes were fabricated via electrospinning and loaded with 2.0% moxifloxacin to achieve the dual functions of axonal guidance and localized antibacterial activity. Morphological characterization revealed uniform fiber diameters, parallel orientation, and interconnected porosity, with favorable mechanical properties and an appropriate hydrophilic-hydrophobic balance. In vitro assays demonstrated Schwann cells maintained sustained proliferation on the membrane surface and exhibited pronounced alignment along the fiber orientation. Antibacterial evaluations showed that the moxifloxacin-loaded ordered PLA membranes achieved nearly complete eradication of bacteria within 2 h, with the mechanism closely associated with disruption of bacterial membrane structures and induction of reactive oxygen species (ROS) accumulation. This work provides a design strategy and experimental basis for multifunctional scaffold materials integrating nerve repair and antibacterial functions.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117353"},"PeriodicalIF":4.5,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573699/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148965924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated 3D light sheet and 2D multiplex imaging for deep histological characterization of somatic mouse glioblastoma. 集成三维光片和二维多重成像，用于躯体小鼠胶质母细胞瘤的深层组织学表征。

IF 4.5 2区综合性期刊

iScience Pub Date : 2026-09-08 eCollection Date: 2026-09-18 DOI: 10.1016/j.isci.2026.117476

Marie Catherine Tiveron, Nathalie Coré, Kevin Bigott, Yliana Huriaux Fontana, Maria Caccavale, Léna Vilvandre, Fabio El Yassouri, Victoria Schoppel, Silvia Rüberg, Melanie Jungblut, Dominique Figarella-Branger, Aurélie Tchoghandjian, Andreas Bosio, Harold Cremer

{"title":"Integrated 3D light sheet and 2D multiplex imaging for deep histological characterization of somatic mouse glioblastoma.","authors":"Marie Catherine Tiveron, Nathalie Coré, Kevin Bigott, Yliana Huriaux Fontana, Maria Caccavale, Léna Vilvandre, Fabio El Yassouri, Victoria Schoppel, Silvia Rüberg, Melanie Jungblut, Dominique Figarella-Branger, Aurélie Tchoghandjian, Andreas Bosio, Harold Cremer","doi":"10.1016/j.isci.2026.117476","DOIUrl":"10.1016/j.isci.2026.117476","url":null,"abstract":"Glioblastoma is a devastating brain cancer for which patient survival has remained largely unchanged for decades, underscoring the need for improved disease modeling and analytical tools. Neural stem cells have been identified as cells of origin of glioblastoma, leading to the development of somatic lineage models. Such models have been deeply characterized by sequencing but systematic histological analyses remain limited. Here, we present a multimodal histological characterization of a somatic glioblastoma mouse model. Using 3D light-sheet imaging, we show that the model is highly reproducible and enables quantitative assessment of tumor growth across cohorts. Through multiplex imaging with MACSima™ Imaging Cyclic Staining, we map the cellular landscape and molecular architecture of the tumors and their environments, and provide a curated resource of mouse-compatible antibodies. Finally, we demonstrate that tissue clearing and light-sheet microscopy can be seamlessly combined with multiplex imaging, enabling spatial proteomic characterization of a 3D pre-defined tumor.","PeriodicalId":342,"journal":{"name":"iScience","volume":"29 9","pages":"117476"},"PeriodicalIF":4.5,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13573706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148965949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0