富含乳酸杆菌的宫颈阴道微生物组与女性较低的BV、HPV和细胞学结果相关

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-09-02 DOI:10.1016/j.isci.2025.113473

John Osei Sekyere , Jason Trama , Martin Adelson , Charulata Trikannad , Desiree DiBlasi , Rachel Schuster , Jing Jing Yang , Eli Mordechai

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引用次数: 0

摘要

宫颈阴道微生物组调节对细菌性阴道病（BV）、高危人乳头瘤病毒（hrHPV）感染和宫颈癌前上皮细胞异常的易感性。我们回顾性分析了15 607例美国妇女（14-95岁；32个州）的qpcr检测的宫颈阴道标本，并整合了微生物组丰度、hrHPV基因分型、pap细胞学和人口统计学。BV在53%的样本中存在，hrHPV在11%的样本中存在。在bv阴性和细胞学正常（NILM）的样本中富集了crispatus、L. gasseri和L. jensenii，而L. iners和bv相关的厌氧菌与hrHPV和细胞学异常共存。机器学习模型证实，年龄、hrHPV状态和crispatus丰度是BV和细胞学结果的最强多变量预测因子（BV AUROC≈0.97）。相互作用分析显示，特定hrHPV基因型与加德纳菌/范尼赫西之间存在协同关联，这进一步增加了细胞学风险。这些发现强调了微生物组分析的临床价值，并支持益生菌策略，促进保护性乳酸杆菌群落减少BV和hrhpv相关的宫颈病理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Lactobacillus-rich cervicovaginal microbiome associated with lower BV, HPV, and cytology outcomes in women

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Lactobacillus-rich cervicovaginal microbiome associated with lower BV, HPV, and cytology outcomes in women

The cervicovaginal microbiome modulates susceptibility to bacterial vaginosis (BV), high-risk human papillomavirus (hrHPV) infection, and epithelial cell abnormalities that precede cervical cancer. We retrospectively analyzed 15 607 qPCR-profiled cervicovaginal specimens from U.S. women (ages 14–95; 32 states) and integrated microbiome abundances, hrHPV genotyping, Pap-cytology, and demographics. BV was present in 53% and hrHPV in 11% of samples. Lactobacillus crispatus, L. gasseri, and L. jensenii were enriched in BV-negative and cytologically normal (NILM) samples, whereas L. iners and BV-associated anaerobes co-occurred with hrHPV and abnormal cytology. Machine-learning models confirmed age, hrHPV status, and L. crispatus abundance as the strongest multivariate predictors of BV and cytological outcomes (BV AUROC ≈0.97). Interaction analyses revealed synergistic associations between specific hrHPV genotypes and Gardnerella/Fannyhessea that further increased cytological risk. These findings underscore the clinical value of microbiome profiling and support probiotic strategies that promote protective Lactobacillus communities to reduce BV and hrHPV-related cervical pathology.

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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