iScience最新文献_第10页

Unveiling Chlamydia trachomatis immunity in urogenital secretions: A systematic review 揭示沙眼衣原体免疫在泌尿生殖系统分泌物：一个系统的回顾

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113453

Anne Van Caesbroeck , Marijana Lipovac , Eef van den Borst , Paolo Palma , Laura Téblick , Alex Vorsters

{"title":"Unveiling Chlamydia trachomatis immunity in urogenital secretions: A systematic review","authors":"Anne Van Caesbroeck , Marijana Lipovac , Eef van den Borst , Paolo Palma , Laura Téblick , Alex Vorsters","doi":"10.1016/j.isci.2025.113453","DOIUrl":"10.1016/j.isci.2025.113453","url":null,"abstract":"<div><div><em>Chlamydia trachomatis</em> (CT) is a highly prevalent bacterial sexually transmitted infection (STI), associated with severe disease complications. CT targets a unique immunological environment: the genital tract epithelium. Although sampling the genital tract is challenging, previous studies have shown that genital CT-specific antibodies exhibit enhanced neutralizing capacity compared with serum antibodies. Furthermore, tissue-resident memory T (TRM) cells provide superior protection compared with circulating T cells. However, further research is required to identify correlates of protection and explore correlations between local and systemic responses. This review provides an overview of the sampling methods suitable for identifying mucosal immune biomarkers associated with CT infection, as well as the immunoassays used. We identified the microbiome, presence of coinfections, hormonal influences, genetics, and CT infection state, load, and genotype as confounding factors to be considered in trial design. Finally, we discuss challenges related to the detection of mucosal immune biomarkers and offer recommendations for future research.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113453"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic optogenetic activation of hippocampal CA1 neurons triggers Alzheimer’s disease-like proteomic remodeling 海马CA1神经元的慢性光遗传学激活引发阿尔茨海默病样蛋白质组重塑

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113454

Iason Keramidis , Martina Samiotaki , Romain Sansonetti , Johanna Alonso , Patrick Desrosiers , Katerina Papanikolopoulou , Yves De Koninck

{"title":"Chronic optogenetic activation of hippocampal CA1 neurons triggers Alzheimer’s disease-like proteomic remodeling","authors":"Iason Keramidis , Martina Samiotaki , Romain Sansonetti , Johanna Alonso , Patrick Desrosiers , Katerina Papanikolopoulou , Yves De Koninck","doi":"10.1016/j.isci.2025.113454","DOIUrl":"10.1016/j.isci.2025.113454","url":null,"abstract":"<div><div>Neuronal overexcitability triggers synaptic changes, leading to neural hyperactivity, network disruption, and is postulated to trigger neurodegeneration in Alzheimer’s disease (AD). However, the sequence of synaptic changes from excessive activity remains unclear. We employed optogenetics to induce sustained neuronal hyperactivity in the hippocampi of wild-type and AD-like 5xFAD mice. After a month of daily optogenetic stimulation, the proteomic profiles of photoactivated wild-type and 5xFAD mice exhibited remarkable similarity. Proteins involved in translation, protein transport, autophagy, and notably in the AD pathology were upregulated in wild-type mice. Conversely, both glutamatergic and GABAergic synaptic proteins were downregulated. These hippocampal proteomic and signaling alterations in wild-type mice resulted in spatial memory loss and augmented Αβ42 secretion. Collectively, these findings indicate that sustained neuronal hyperactivity alone replicates proteome changes seen in AD-like mutant mice. Therefore, prolonged neuronal hyperactivity may contribute to synaptic transmission disruption, memory deficits and the neurodegenerative process associated with AD.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113454"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of CCL20-driven CAR-γδ T secreting PD-1 blockade with enhanced trafficking into solid tumor ccl20驱动的CAR-γδ T分泌PD-1阻断增强实体瘤运输的验证

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113463

Dengji Zhang , Yuan Tang , Wei Sun , Yunkun Guan , Yipeng Cheng , Yuqi Zhu , Xiaying Zhao , Xinyi Yang , Huanzhang Zhu

{"title":"Validation of CCL20-driven CAR-γδ T secreting PD-1 blockade with enhanced trafficking into solid tumor","authors":"Dengji Zhang , Yuan Tang , Wei Sun , Yunkun Guan , Yipeng Cheng , Yuqi Zhu , Xiaying Zhao , Xinyi Yang , Huanzhang Zhu","doi":"10.1016/j.isci.2025.113463","DOIUrl":"10.1016/j.isci.2025.113463","url":null,"abstract":"<div><div>The primary challenges of CAR-T cells for solid tumor treatment involve the efficient delivery and infiltration of CAR-T cells into the tumor site, as well as overcoming the immunosuppressive tumor microenvironment. Combining chemokine-guided trafficking and delivery with the clearance of immunosuppressive barriers represents a promising strategy. An EGFR-targeted CAR-γδ T, CAR-E276 that co-expresses CCR6 and secretes PD1 blockade was <em>in vivo</em> validated using a non-small cell lung cancer (NSCLC) CDX model. The results from CAR-E276 indicated that integrating chemokines, checkpoint blockades, and γδ T cell properties into CAR-T cells facilitated their efficient trafficking into tumor tissues, ensured prolonged persistence, and achieved robust tumor-killing effects without inducing GvHD. Notably, CAR-E276 also exhibited potential for off-the-shelf and allogeneic applications. These findings are expected to offer valuable insights and drive the development of CAR-T therapies targeting solid tumors.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113463"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular lactate dynamics in Drosophila neurons 果蝇神经元细胞内乳酸动力学

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113462

Matthew S. Price , Elham Rastegari , Richa Gupta , Katie Vo , Travis I. Moore , Kartik Venkatachalam

{"title":"Intracellular lactate dynamics in Drosophila neurons","authors":"Matthew S. Price , Elham Rastegari , Richa Gupta , Katie Vo , Travis I. Moore , Kartik Venkatachalam","doi":"10.1016/j.isci.2025.113462","DOIUrl":"10.1016/j.isci.2025.113462","url":null,"abstract":"<div><div>Rates of lactate production and consumption reflect the metabolic state of many cell types, including neurons. Here, we investigated the effects of nutrient deprivation on lactate dynamics in <em>Drosophila</em> neurons by leveraging the limiting effects of the unstirred layer, a diffusion barrier that forms around cells in culture. We found that neurons constitutively consume lactate when the availability of trehalose, a glucose disaccharide preferred by insects, is limited by the unstirred layer. Acute mechanical disruption of the unstirred layer reduced this reliance on lactate. Through kinetic modeling and experimental validation, we demonstrate that neuronal lactate consumption rates under unstirred layer conditions correlate inversely with the cells’ mitochondrial density. Furthermore, neuronal lactate levels exhibited temporal correlations that allowed postperturbation lactate dynamics to be predicted by preperturbation values. Collectively, our findings reveal the influence of naturally forming diffusion barriers on neuronal metabolic preferences and demonstrate the existence of temporal correlations between lactate dynamics under conditions of nutrient deprivation and those evoked by the subsequent restoration of nutrient availability.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113462"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New insights into the influence of temperature on axonal transport and function in myelinated regions of Schwann cells 温度对雪旺细胞髓鞘区轴突运输和功能影响的新见解

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113467

Chih-Wei Zeng , Yasuhiro Kamei

{"title":"New insights into the influence of temperature on axonal transport and function in myelinated regions of Schwann cells","authors":"Chih-Wei Zeng , Yasuhiro Kamei","doi":"10.1016/j.isci.2025.113467","DOIUrl":"10.1016/j.isci.2025.113467","url":null,"abstract":"<div><div>Temperature within the range of 15°C–37°C plays a pivotal role in modulating cellular processes and is essential for understanding the complex mechanisms underlying axonal transport and function in myelinated regions of Schwann cells (SCs). This review presents a comprehensive overview of the current knowledge on the impact of temperature on various aspects of axonal function, including saltatory conduction, ion channel activity, molecular motor dynamics, and Schwann cell function. We also delve into the potential implications of these findings in the context of neurological disorders and their treatment. The temperature-dependent nature of saltatory conduction and action potential propagation in myelinated axons is of particular interest, as it directly affects the efficiency of nerve signal transmission. Additionally, the activity of ion channels in the nodes of Ranvier is subject to modulation by temperature, further emphasizing the importance of understanding temperature’s influence on neuronal function. This review concludes with a discussion of various unresolved questions in the field, and ideas are suggested for future research. Studying the precise molecular mechanisms underlying the temperature-dependent regulation of ion channels, molecular motors, and cytoskeletal components may lead to the development of novel strategies for the diagnosis and treatment of neurological disorders, which are commonly observed in demyelinating diseases and hereditary neuropathies. A deeper understanding of the role of temperature in neuronal function has the potential to significantly advance our knowledge of thermoregulation and neurologic function, ultimately leading to breakthroughs in the diagnosis and treatment of various neurological disorders.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113467"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Measles trend in Gabon: Insight from 18 years of surveillance data 加蓬的麻疹趋势：来自18年监测数据的洞察

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-27 DOI: 10.1016/j.isci.2025.113450

Guy Francis Nzengui Nzengui , Hervé M’boyis Kamdem , Ayawa Claudine Kombila Koumavor , Gaël Mourembou , Pélagie Mougola Bissiengou , Daniella Delpo , Rotimi Myrabelle Avome Houechenou , Fabrice Lotola Mougeni , Fortune Barondi Diambou , Annie Badila Missogni , Frédéric Ngoulou , Marcel Mouketou Mabika , Thierry Mba , Alain Mombo Mombo , Paulin Mbagou , Urlucha Marina Mbani Okoumba , Alyssa Sbarra , Saki Takahashi , Anicet Christel Maloupazoa Siawaya , Amandine Mveang Nzoghe , Angélique Ndjoyi Mbiguino

{"title":"Measles trend in Gabon: Insight from 18 years of surveillance data","authors":"Guy Francis Nzengui Nzengui , Hervé M’boyis Kamdem , Ayawa Claudine Kombila Koumavor , Gaël Mourembou , Pélagie Mougola Bissiengou , Daniella Delpo , Rotimi Myrabelle Avome Houechenou , Fabrice Lotola Mougeni , Fortune Barondi Diambou , Annie Badila Missogni , Frédéric Ngoulou , Marcel Mouketou Mabika , Thierry Mba , Alain Mombo Mombo , Paulin Mbagou , Urlucha Marina Mbani Okoumba , Alyssa Sbarra , Saki Takahashi , Anicet Christel Maloupazoa Siawaya , Amandine Mveang Nzoghe , Angélique Ndjoyi Mbiguino","doi":"10.1016/j.isci.2025.113450","DOIUrl":"10.1016/j.isci.2025.113450","url":null,"abstract":"<div><div>Measles is a vaccine-preventable infection that can lead to serious illness and death. The delayed implementation of the World Health Organization (WHO)-recommended two doses of measles-containing vaccine (MCV1 and MCV2) and immunity gaps compromise the effort to elimination. Eighteen years (2006–2023) of surveillance data from Gabon measles surveillance program were collected and analyzed. Variables included age, sex, vaccination status and date, disease date, and anti-measles immunoglobulin M (IgM) status (determined by immunoassay). The rate of infection and association studies was done. Results showed that 60.6% (1,640/2,708) of suspected cases were unvaccinated against measles. Of suspected cases, 26.1% were laboratory-confirmed cases. Unvaccinated individuals were 5.9–7.7 times more likely to be anti-measles IgM positive (<em>p</em> < 0.0001). Children aged ≤5 years represented 70.8% of laboratory-confirmed cases; 15.5% (30/193) of childbearing age females were confirmed cases. In Gabon, nonimmunized and underimmunized individuals are driving up measles disease and constitute a challenge to measles elimination.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113450"},"PeriodicalIF":4.1,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endolysosomal dysfunction impairs proteostasis and induces neurodegeneration in vivo 体内内溶酶体功能障碍损害蛋白质平衡并诱导神经退行性变

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-27 DOI: 10.1016/j.isci.2025.113460

Wei Shao , Ellen A. Albagli , Karen Jansen-West , Lillian M. Daughrity , Jimei Tong , Anxhela Hysi , Peizhou Jiang , Tiffany W. Todd , Emma Lee , Desiree Zanetti Alepuz , Yari Carlomagno , Mei Yue , Judith A. Dunmore , Monica Castanedes-Casey , Paula Castellanos Otero , Aishe Kurti , Mercedes Prudencio , Casey N. Cook , Dennis W. Dickson , Tania F. Gendron , Yong-Jie Zhang

{"title":"Endolysosomal dysfunction impairs proteostasis and induces neurodegeneration in vivo","authors":"Wei Shao , Ellen A. Albagli , Karen Jansen-West , Lillian M. Daughrity , Jimei Tong , Anxhela Hysi , Peizhou Jiang , Tiffany W. Todd , Emma Lee , Desiree Zanetti Alepuz , Yari Carlomagno , Mei Yue , Judith A. Dunmore , Monica Castanedes-Casey , Paula Castellanos Otero , Aishe Kurti , Mercedes Prudencio , Casey N. Cook , Dennis W. Dickson , Tania F. Gendron , Yong-Jie Zhang","doi":"10.1016/j.isci.2025.113460","DOIUrl":"10.1016/j.isci.2025.113460","url":null,"abstract":"<div><div>Transactive response (TAR) DNA-binding protein 43 (TDP-43) inclusions are a pathological hallmark of the frontotemporal dementia (FTD)-amyotrophic lateral sclerosis (ALS) spectrum. Dysfunction of the endolysosomal system, which plays a crucial role in protein trafficking and maintaining proteostasis, has been implicated in FTD-ALS pathogenesis. While the impact of endolysosomal dysfunction on TDP-43 pathology remains unclear, we demonstrated that disrupting the endolysosomal pathway by expressing the constitutively active endosomal protein, Rab5<sup>Q79L</sup>, induces TDP-43 aggregation in cultured cells. Here, we generated a mouse model expressing GFP-tagged Rab5<sup>Q79L</sup>, demonstrating that GFP-Rab5<sup>Q79L</sup> mice exhibit early motor deficits and endolysosomal dysfunction, including enlarged endosomes, abnormal lysosome morphology, and p62- or ubiquitin-positive inclusions. These mice also developed significant neuronal loss, neuroinflammation, phosphorylated TDP-43 (pTDP-43) inclusions, and nuclear envelope and nuclear pore structural defects reminiscent of FTD-ALS. Accordingly, GFP-Rab5<sup>Q79L</sup> mice will prove useful in expanding our understanding of endolysosomal dysfunction in proteostasis and pTDP-43 pathology.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113460"},"PeriodicalIF":4.1,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic-mediated positive feedback loop facilitates the progression of lung adenocarcinoma 表观遗传介导的正反馈环促进肺腺癌的进展

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-26 DOI: 10.1016/j.isci.2025.113339

Yihang Cheng , Yifeng Luo , Tingting Hu , Qiaoling Ren , Li Xu , Tuo Yi , Yuan Tan , Wei Li , Xuxin Wang , Yaoxiang Sun , Mingzhi Chen , Zhonghua Shen , Bin Zhang , Youhuang Bai , Yue Tao , Zhihong Cao , Deqiang Sun

{"title":"Epigenetic-mediated positive feedback loop facilitates the progression of lung adenocarcinoma","authors":"Yihang Cheng , Yifeng Luo , Tingting Hu , Qiaoling Ren , Li Xu , Tuo Yi , Yuan Tan , Wei Li , Xuxin Wang , Yaoxiang Sun , Mingzhi Chen , Zhonghua Shen , Bin Zhang , Youhuang Bai , Yue Tao , Zhihong Cao , Deqiang Sun","doi":"10.1016/j.isci.2025.113339","DOIUrl":"10.1016/j.isci.2025.113339","url":null,"abstract":"<div><div>Lung adenocarcinoma (LUAD) remains a leading cause of cancer-related mortality. We performed multi-omics approaches on LUAD samples spanning different stages, establishing a detailed epigenetic landscape. We identified 1,416 regions characterized by hypo-DNA methylation and hyperchromatin accessibility associated with high mortality and recurrence rates, serving as a biomarker panel for LUAD staging and diagnosis (AUC of 0.89 and 0.87 for two-class and multi-class models). Epigenetics-related transcription factors, especially the AP-1 family, were observed at these loci, and transcriptomic profiling indicated persistent activation of the mitogen-activated protein kinase (MAPK) signaling pathway. Hypomethylation and hyperaccessibility of AP-1 binding sites enhance EGFR and FGFBP1 expression, activating the MAPK pathway. This reveals an epigenetic-mediated positive feedback loop, MAPK→AP-1/Epigenetic Modifications→EGFR/FGFBP1→MAPK, in LUAD, highlighting the complex interplay between epigenetic modifications and LUAD progression, offering potential detection and treatment targets. These findings define a high-resolution, multi-stage epigenetic landscape of LUAD, providing a resource for biomarker discovery and mechanistic insight.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113339"},"PeriodicalIF":4.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

International multicenter development of ensemble machine learning driven host response based diagnosis for tuberculosis. 集成机器学习驱动的基于宿主反应的结核病诊断的国际多中心发展。

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-26 eCollection Date: 2025-09-19 DOI: 10.1016/j.isci.2025.113444

Shufa Zheng, Wenxin Qu, Dan Zhang, Jieting Zhou, Yifan Xu, Wei Wu, Chang Liu, Mingzhu Huang, Enhui Shen, Xiao Chen, Michael P Timko, Longjiang Fan, Fei Yu, Dongsheng Han, Yifei Shen

{"title":"International multicenter development of ensemble machine learning driven host response based diagnosis for tuberculosis.","authors":"Shufa Zheng, Wenxin Qu, Dan Zhang, Jieting Zhou, Yifan Xu, Wei Wu, Chang Liu, Mingzhu Huang, Enhui Shen, Xiao Chen, Michael P Timko, Longjiang Fan, Fei Yu, Dongsheng Han, Yifei Shen","doi":"10.1016/j.isci.2025.113444","DOIUrl":"10.1016/j.isci.2025.113444","url":null,"abstract":"<p><p>Active pulmonary tuberculosis (TB) is challenging to diagnose, and monitoring treatment response effectively remains difficult. To address these challenges, we developed TB-Scope, a host-gene-expression-based ensemble machine learning classification model. Using large-scale microarray datasets (<i>N</i> = 1,258) from three retrospective transcriptomic studies, we selected 143 feature genes (biomarkers) based on their expression ranks to predict ATB. The Top Scoring Pairs (TSP) ensemble classifier for ATB diagnosis was optimized using multi-cohort training samples. We then combined the ATB/Health, ATB/LTBI, and ATB/ODs classifiers to construct an ATB diagnosis decision model (TB-Scope decision). To assess the performance of the TB-Scope algorithm and decision model, we analyzed 12 independent microarray and RNA-seq validation datasets (<i>N</i> = 1,786) comprising both children and adults from seven countries. Thus, our data demonstrates that TB-Scope provides a powerful and reliable tool for accurately diagnosing ATB across diverse data platforms.</p>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 9","pages":"113444"},"PeriodicalIF":4.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12455115/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unpacking Ross River virus trends in Queensland: Regional insights and predictive modeling 解开罗斯河病毒在昆士兰的趋势：区域见解和预测建模

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-26 DOI: 10.1016/j.isci.2025.113446

Wei Qian , Kathryn Glass , David Harley , Elvina Viennet , Cameron Hurst

{"title":"Unpacking Ross River virus trends in Queensland: Regional insights and predictive modeling","authors":"Wei Qian , Kathryn Glass , David Harley , Elvina Viennet , Cameron Hurst","doi":"10.1016/j.isci.2025.113446","DOIUrl":"10.1016/j.isci.2025.113446","url":null,"abstract":"<div><div>Ross River virus (RRV) transmission cycle varies across Queensland, Australia. Identifying key exposures associated with regional RRV incidence can aid disease prevention and control. Using RRV notification data from 2001 to 2020, we developed negative binomial generalized linear models to predict RRV incidence across three Queensland regions categorized by thermal climate zones (Hot, Warm, and Dry). Predictors and RRV trends differed across regions, indicating unique transmission patterns: seasonally driven in the Hot region, outbreak driven in the Warm region, and a mixed transmission pattern in the Dry region. The important predictors included recent RRV cases, normalized difference vegetation index (vegetation cover density), accessibility/remoteness index of Australia scores (indicating remoteness), and land use proportions. These findings enhance the understanding of local RRV transmission dynamics, highlight primary environmental drivers of transmission, and inform targeted disease management strategies. This approach may also benefit studies on other vector-borne diseases with complex transmission cycles in varying environments and climates.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113446"},"PeriodicalIF":4.1,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0