iScience最新文献_第9页

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.112891

Yuqing Lin, Maria Mytiliniou, Kanudha Sharda, Tina Zhang

引用次数: 0

Macrophage fatty acid metabolism under chronic hypoxia shapes γδ T cell recruitment via CCL22 慢性缺氧下巨噬细胞脂肪酸代谢通过CCL22影响γδ T细胞募集

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.113025

Vanesa M. Guerrero Ruiz , Aya Khabaza , Rebekka Bauer , Blerina Aliraj , Siavash Mansouri , Sofie P. Meyer , Megan A. Palmer , Hauke Winter , Laura V. Klotz , Mohammed A.F. Elewa , Ivan M. Kur , Carlo Angioni , Rahmat Mojaradfar , Lisa Hahnefeld , Rajkumar Savai , Dominik Fuhrmann , Andreas Weigert , Bernhard Brüne

{"title":"Macrophage fatty acid metabolism under chronic hypoxia shapes γδ T cell recruitment via CCL22","authors":"Vanesa M. Guerrero Ruiz , Aya Khabaza , Rebekka Bauer , Blerina Aliraj , Siavash Mansouri , Sofie P. Meyer , Megan A. Palmer , Hauke Winter , Laura V. Klotz , Mohammed A.F. Elewa , Ivan M. Kur , Carlo Angioni , Rahmat Mojaradfar , Lisa Hahnefeld , Rajkumar Savai , Dominik Fuhrmann , Andreas Weigert , Bernhard Brüne","doi":"10.1016/j.isci.2025.113025","DOIUrl":"10.1016/j.isci.2025.113025","url":null,"abstract":"<div><div>Hypoxia in solid tumors is associated with poor outcomes because of metabolic adaptations that support tumor cell survival and alter immune cell function. However, the metabolic and phenotypic adaptations of macrophages (MФs) to chronic hypoxia (CH) remain unclear. This study identifies impaired activity of the oxygen-dependent enzyme stearoyl-CoA desaturase 1 (SCD1) as a driver of altered fatty acid (FA) metabolism in MФs under CH. SCD1 deletion enhanced pro-inflammatory gene expression while suppressing the production of the chemokine CCL22. We propose that attenuated SCD1 activity and an altered saturated fatty acids (SFA)/monounsaturated fatty acids (MUFA) ratio impair the function of the transcription factor HNF4α, thereby affecting the expression of inflammatory genes such as CCL22. Reduced CCL22 levels, in turn, impaired γδ T cell recruitment. Accordingly, CCL22 expression in non-small cell lung cancer patients correlated positively with γδ T cell frequency and patient survival. These findings highlight the immunometabolic role of SCD1 in the hypoxic tumor microenvironment.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 8","pages":"Article 113025"},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and toxicity risks of radiotherapy combined with PD-1/PD-L1 inhibitors: A comprehensive review 放疗联合PD-1/PD-L1抑制剂的安全性和毒性风险：综合综述

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.112882

Lijing Zeng , Jing Yang , Huang Xia , Zeyuan Li , Yu Lin , Qiwei Yao , Rong Zheng

引用次数: 0

A genetic safeguard for eliminating target genes in synthetic probiotics in a gut environment 消除肠道环境中合成益生菌中靶基因的遗传保障

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.113027

Nhu Nguyen , Miaomiao Wang , Lin Li , Clement T.Y. Chan

引用次数: 0

Improved soil salinity estimation in arid regions: Leveraging bare soil periods and environmental factors 干旱区改良土壤盐度估算：利用裸地期和环境因素

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.113020

Weitong Ma , Xin Cui , Wenting Han , Huihui Zhang , Liyuan Zhang

{"title":"Improved soil salinity estimation in arid regions: Leveraging bare soil periods and environmental factors","authors":"Weitong Ma , Xin Cui , Wenting Han , Huihui Zhang , Liyuan Zhang","doi":"10.1016/j.isci.2025.113020","DOIUrl":"10.1016/j.isci.2025.113020","url":null,"abstract":"<div><div>In the context of global land degradation and increasing salinization of cultivated land, accurately and sustainably estimating soil salinization is essential for effective land management. This study explores a novel approach to improve soil salt content (SSC) monitoring by minimizing the influence of long-term environmental variability and incorporating relevant environmental factors. Focusing on exposed cultivated land in arid regions, we analyzed the sensitivity of multispectral indices and environmental factors to SSC during pre-seeding and post-harvest bare soil periods. Our findings indicate that soil mechanical composition and salinity indices exhibited strong correlations with SSC, which further enhanced when the periods were analyzed separately. Dividing the bare soil periods improved the model performance, increasing R<sup>2</sup> by 10.2%–55.7%, and the support vector regression model performed better, with an R<sup>2</sup> of 0.77, RMSE of 0.11%. This approach offers a robust framework for precision agriculture and sustainable land management in salinity-affected regions.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 8","pages":"Article 113020"},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative multi-omics characterization of 12 syngeneic mouse models 12种同基因小鼠模型的综合多组学研究

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.113024

Zihan Xu , Binchen Mao , Hengyuan Liu , Shijia Wang , Xiaobo Chen , Sheng Guo

{"title":"Integrative multi-omics characterization of 12 syngeneic mouse models","authors":"Zihan Xu , Binchen Mao , Hengyuan Liu , Shijia Wang , Xiaobo Chen , Sheng Guo","doi":"10.1016/j.isci.2025.113024","DOIUrl":"10.1016/j.isci.2025.113024","url":null,"abstract":"<div><div>Mouse syngeneic models serve as indispensable tools for elucidating tumor-immune interactions and assessing immunotherapy efficacy. In this study, we first conducted a comprehensive evaluation of six label-free protein quantification pipelines across 12 mouse syngeneic models, revealing that data-independent acquisition (DIA) significantly outperforms data-dependent acquisition (DDA) in terms of data coverage, reproducibility, and inter-model discrimination. We next performed an integrative multi-omics analysis to uncover molecular mechanisms associated with treatment response. Our analysis identified Dnmt3a and Igf2r, which are correlated with resistance to immune checkpoint inhibitors (ICIs), and highlighted key pathways including interferon signaling and oxidative phosphorylation that distinguish responders from non-responders. To facilitate broader research applications, we have developed an interactive web resource that shares our multi-omics datasets and analytical results, equipped with user-friendly tools for further exploration. This resource aims to accelerate preclinical research and contribute to the development of personalized cancer therapies.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 8","pages":"Article 113024"},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of host genes by nested genes during C. elegans development 线虫发育过程中巢式基因对宿主基因的诱导

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.113021

Fabien Soulavie , Carole Couillault , Matéo Melki , Khulganaa Buyannemekh , Antoine Barrière , Paul Villoutreix , Vincent Bertrand

{"title":"Induction of host genes by nested genes during C. elegans development","authors":"Fabien Soulavie , Carole Couillault , Matéo Melki , Khulganaa Buyannemekh , Antoine Barrière , Paul Villoutreix , Vincent Bertrand","doi":"10.1016/j.isci.2025.113021","DOIUrl":"10.1016/j.isci.2025.113021","url":null,"abstract":"<div><div>Embryonic development relies on tightly controlled gene expression. In this study, we analyzed the transcriptional effect during <em>C. elegans</em> development of a striking genomic topology, the opposite nested configuration, where a gene is located in an intron of a host gene in the opposite direction. Using CRISPR genome engineering and single molecule FISH, we characterized the regulatory interactions between <em>ceh-10</em>, a transcription factor involved in neuronal specification, and its host <em>polq-1</em>, a DNA repair enzyme, showing that the nested gene induces transcription of a short version of its host in neurons. Extending our analysis to the hundreds of protein coding genes in opposite nested configuration and using single-cell RNA-seq data covering <em>C. elegans</em> embryogenesis, we observed that coexpression between nested and host genes is relatively common especially in cells positive for the nested gene. Our study illustrates how the presence of a nested gene can influence expression of its host.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 8","pages":"Article 113021"},"PeriodicalIF":4.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144605201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus 外苍白球分子定义神经元的细胞和突触特性

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-27 DOI: 10.1016/j.isci.2025.113002

Linda M.C. Koene , Wilhelm Thunberg , Sten Grillner , Gilad Silberberg , Maya Ketzef

引用次数: 0

Analysis of clinical characteristics and factors of central nervous system damage caused by acute bipyridine herbicide poisoning 联吡啶类除草剂急性中毒致中枢神经系统损害的临床特点及影响因素分析

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-26 DOI: 10.1016/j.isci.2025.112851

Hu Tang , Xiaorong Chen , Nengzhi Xia , Bin Wu , Guangju Zhao , Yahui Tang , Zhongqiu Lu

{"title":"Analysis of clinical characteristics and factors of central nervous system damage caused by acute bipyridine herbicide poisoning","authors":"Hu Tang , Xiaorong Chen , Nengzhi Xia , Bin Wu , Guangju Zhao , Yahui Tang , Zhongqiu Lu","doi":"10.1016/j.isci.2025.112851","DOIUrl":"10.1016/j.isci.2025.112851","url":null,"abstract":"<div><div>Acute bipyridine herbicide poisoning poses a significant clinical challenge due to the lack of an effective antidote, with CNS damage leading to particularly poor outcomes. This retrospective study included 189 patients with acute bipyridine poisoning, with a median age of 39 years. Among them, 33.3% developed CNS damage, with a median onset time of 48 h, and overall mortality was 50.8%. Correlation analyses revealed that both diquat (DQ) and paraquat (PQ) poisoning were associated with systemic damage. However, DQ poisoning showed additional associations with inflammatory markers (WBC [white blood cell], neutrophils), liver dysfunction (alanine aminotransferase [ALT]), and CNS damage, underscoring its broader systemic impact. To address the need for early identification of CNS damage, we developed a robust 8-factor predictive model. Its performance, validated through receiver operating characteristic (ROC) curve analysis, calibration curves, and decision curve analysis (DCA), demonstrated strong clinical potential for improving early risk stratification and guiding targeted interventions.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 8","pages":"Article 112851"},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary and metastatic cellular landscapes in human pancreatic cancer 人胰腺癌的原发性和转移性细胞景观

IF 4.6 2区综合性期刊

iScience Pub Date : 2025-06-26 DOI: 10.1016/j.isci.2025.113012

Nina G. Steele , Veerin R. Sirihorachai , Ahmed M. Elhossiny , Ian M. Loveless , Padma Kadiyala , Monica Bonilla , Emily L. Lasse-Opsahl , Carinna Solano Vargas , Katelyn L. Donahue , Samantha B. Kemp , Valerie Gunchick , Yatrik M. Shah , Timothy L. Frankel , Filip Bednar , Arvind Rao , Benjamin L. Allen , Jiaqi Shi , Vaibhav Sahai , Howard C. Crawford , Eileen S. Carpenter , Marina Pasca di Magliano

{"title":"Primary and metastatic cellular landscapes in human pancreatic cancer","authors":"Nina G. Steele , Veerin R. Sirihorachai , Ahmed M. Elhossiny , Ian M. Loveless , Padma Kadiyala , Monica Bonilla , Emily L. Lasse-Opsahl , Carinna Solano Vargas , Katelyn L. Donahue , Samantha B. Kemp , Valerie Gunchick , Yatrik M. Shah , Timothy L. Frankel , Filip Bednar , Arvind Rao , Benjamin L. Allen , Jiaqi Shi , Vaibhav Sahai , Howard C. Crawford , Eileen S. Carpenter , Marina Pasca di Magliano","doi":"10.1016/j.isci.2025.113012","DOIUrl":"10.1016/j.isci.2025.113012","url":null,"abstract":"<div><div>Pancreatic ductal adenocarcinoma (PDAC) is characterized by a complex tumor microenvironment (TME). We utilized single cell RNA sequencing to compare the TMEs of metastatic sites and primary tumors. We detected increased prevalence of exhausted CD8<sup>+</sup> T cells in metastases, as well as the enrichment of complement pathway encoding genes in immunosuppressive tumor-associated macrophages, consistent with profound immunosuppression in metastatic disease. In cancer-associated fibroblasts, we identified a unique upregulation of metabolic genes, including UPP1, in metastasis. In cancer cells, we uncovered a specific gene signature upregulated in liver metastases; this signature was present in a proportion of primary tumors in the TCGA dataset, where it correlated with worse survival. Overall, our analysis of primary and metastatic PDAC defines a “high-risk” gene signature, metabolic reprogramming, and increased immune suppression in metastasis.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 8","pages":"Article 113012"},"PeriodicalIF":4.6,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144633633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0