iScience最新文献_第9页

Evidences from gut microbiome and habitat landscape indicate continued threat of extinction for the Hainan gibbon 来自肠道微生物组和栖息地景观的证据表明，海南长臂猿仍面临灭绝的威胁

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-08 DOI: 10.1016/j.isci.2024.111352

Guangping Huang , Wei Li , Yun Liu , Jiang Zhou , Fuwen Wei

引用次数: 0

Berbamine prevents SARS-CoV-2 entry and transmission 小檗胺可预防 SARS-CoV-2 的进入和传播

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-08 DOI: 10.1016/j.isci.2024.111347

Srikanth Sadhu , Sandeep Goswami , Ritika Khatri , Bharat Lohiya , Virendra Singh , Rahul Yadav , Vinayaka Das , Manas Ranjan Tripathy , Prabhanjan Dwivedi , Mitul Srivastava , Shailendra Mani , Shailendra Asthana , Sweety Samal , Amit Awasthi

{"title":"Berbamine prevents SARS-CoV-2 entry and transmission","authors":"Srikanth Sadhu , Sandeep Goswami , Ritika Khatri , Bharat Lohiya , Virendra Singh , Rahul Yadav , Vinayaka Das , Manas Ranjan Tripathy , Prabhanjan Dwivedi , Mitul Srivastava , Shailendra Mani , Shailendra Asthana , Sweety Samal , Amit Awasthi","doi":"10.1016/j.isci.2024.111347","DOIUrl":"10.1016/j.isci.2024.111347","url":null,"abstract":"<div><div>Effective antiviral drugs are essential to combat COVID-19 and future pandemics. Although many compounds show antiviral <em>in vitro</em> activity, only a few retain effectiveness <em>in vivo</em> against SARS-CoV-2. Here, we show that berbamine (Berb) is effective against SARS-CoV, MER-CoV, SARS-CoV-2 and its variants, including the XBB.1.16 variant. In hACE2.Tg mice, Berb suppresses SARS-CoV-2 replication through two distinct mechanisms: inhibiting spike-mediated viral entry and enhancing antiviral gene expression during infection. The administration of Berb, in combination with remdesivir (RDV), clofazimine (Clof) and fangchinoline (Fcn), nearly eliminated viral load and promoted recovery from acute SARS-CoV-2 infection and its variants. Co-housed mice in direct contact with either pre-treated or untreated infected mice exhibited negligible viral loads, reduced lung pathology, and decreased viral shedding, suggesting that Berb may effectively hinder virus transmission. This broad-spectrum activity positions Berb as a promising preventive or therapeutic option against betacoronaviruses.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111347"},"PeriodicalIF":4.6,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Audience presence influences cognitive task performance in chimpanzees.

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-08 eCollection Date: 2024-11-15 DOI: 10.1016/j.isci.2024.111191

Christen Lin, Akiho Muramatsu, Shinya Yamamoto

引用次数: 0

Assessing the impact of armed conflict on the progress of achieving 17 sustainable development goals 评估武装冲突对实现 17 项可持续发展目标的影响

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-07 DOI: 10.1016/j.isci.2024.111331

Di Wang , Mengmeng Hao , Ning Li , Dong Jiang

引用次数: 0

The role of the tricellular junction protein ILDR2 in glomerulopathies: Expression patterns and functional insights 三细胞连接蛋白 ILDR2 在肾小球疾病中的作用：表达模式与功能见解

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-07 DOI: 10.1016/j.isci.2024.111329

Florian Siegerist , Felix Kliewe , Elke Hammer , Paul Schakau , Joanne Ern Chi Soh , Claudia Weber , Maja Lindenmeyer , Simone Reichelt-Wurm , Vedran Drenic , Christos Chatziantoniou , Christos E. Chadjichristos , Yiying Zhang , Stefan Simm , Miriam C. Banas , Matthias Nauck , Uwe Völker , Nicole Endlich

{"title":"The role of the tricellular junction protein ILDR2 in glomerulopathies: Expression patterns and functional insights","authors":"Florian Siegerist , Felix Kliewe , Elke Hammer , Paul Schakau , Joanne Ern Chi Soh , Claudia Weber , Maja Lindenmeyer , Simone Reichelt-Wurm , Vedran Drenic , Christos Chatziantoniou , Christos E. Chadjichristos , Yiying Zhang , Stefan Simm , Miriam C. Banas , Matthias Nauck , Uwe Völker , Nicole Endlich","doi":"10.1016/j.isci.2024.111329","DOIUrl":"10.1016/j.isci.2024.111329","url":null,"abstract":"<div><div>The tricellular tight junctions are crucial for the regulation of paracellular flux at tricellular junctions, where tricellulin (MARVELD2) and angulins (ILDR1, ILDR2, or LSR) are localized. The role of ILDR2 in podocytes, specialized epithelial cells in the kidney, is still unknown. We investigated the role of ILDR2 in glomeruli and its influence on blood filtration. Western blots, single-cell RNA sequencing (scRNA-seq), and superresolution microscopy showed a strong expression of ILDR2 in podocytes that colocalized with the podocyte-specific claudin CLDN5. Co-immunoprecipitation revealed that ILDR2 interacts with CLDN5. In glomerulopathies, induced by nephrotoxic serum and by desoxycorticosterone acetate (DOCA)-salt heminephrectomy, ILDR2 was strongly up-regulated. Furthermore, <em>Ildr2</em> knockout mice exhibited glomerular hypertrophy and decreased podocyte density. However, they did not develop effacement of podocyte foot processes or proteinuria. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) proteomic analysis of isolated glomeruli showed an increase in matrix proteins, such as fibronectin and collagens. This suggests a protective role of ILDR2 in glomerulopathies.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111329"},"PeriodicalIF":4.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A lysosome-targeted fluorescent probe with large Stokes shift for visualizing biothiols in vivo and in vitro 具有大斯托克斯偏移的溶酶体靶向荧光探针，用于在体内和体外观察生物硫醇

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-07 DOI: 10.1016/j.isci.2024.111334

Taotao Zhao , Tong Zhang , Zijun Tao , Zhe Zhou , Xiaofeng Xia , Zhengjun Wu , Feiyi Wang , Jun Ren , Erfei Wang

引用次数: 0

Influence of myosteatosis on survival of patients with pancreatic cancer: A systematic review and meta-analysis 肌骨质疏松症对胰腺癌患者生存期的影响：系统回顾与荟萃分析

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-07 DOI: 10.1016/j.isci.2024.111343

Xin Zhang , Licheng Wei , Jiangguo Li , Yuexia Deng , Wei Xu , Dongkui Chen , Xing Li

{"title":"Influence of myosteatosis on survival of patients with pancreatic cancer: A systematic review and meta-analysis","authors":"Xin Zhang , Licheng Wei , Jiangguo Li , Yuexia Deng , Wei Xu , Dongkui Chen , Xing Li","doi":"10.1016/j.isci.2024.111343","DOIUrl":"10.1016/j.isci.2024.111343","url":null,"abstract":"<div><div>The present meta-analysis aims to evaluate the impact of myosteatosis on overall survival (OS) and progression-free survival (PFS) in patients with pancreatic cancer (PC). A comprehensive literature search was conducted in the Medline, Web of Science, and Embase databases. The hazard ratio (HR) and corresponding 95% confidence interval (CI) for the association between myosteatosis and survival outcomes were pooled using a random-effects model. A total of 14 studies were included. The pooled analysis demonstrated that myosteatosis was significantly associated to poorer OS (HR: 1.50, 95% CI: 1.35–1.67, <em>p</em> < 0.001; <em>I</em><sup><em>2</em></sup> = 0%). The subgroup analysis revealed consistent results across various study characteristics, including geographic regions, cancer stages, follow-up durations, and study quality. In addition, myosteatosis was associated to worse PFS (HR: 1.34, 95% CI: 1.15–1.57, <em>p</em> < 0.001; <em>I</em><sup><em>2</em></sup> = 34%). The present meta-analysis indicates that myosteatosis is associated to significantly worse OS and PFS in patients with PC.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111343"},"PeriodicalIF":4.6,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes 基于水凝胶珠的等温检测（BEAD-ID）用于评估 DNA 修饰酶的活性

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-06 DOI: 10.1016/j.isci.2024.111332

Kathrine Nygaard Borg , Ayush Shetty , Guangyao Cheng , Shaodi Zhu , Tianle Wang , Wu Yuan , Ho Pui Ho , Birgitta Ruth Knudsen , Cinzia Tesauro , Yi-Ping Ho

{"title":"Hydrogel bead-based isothermal detection (BEAD-ID) for assessing the activity of DNA-modifying enzymes","authors":"Kathrine Nygaard Borg , Ayush Shetty , Guangyao Cheng , Shaodi Zhu , Tianle Wang , Wu Yuan , Ho Pui Ho , Birgitta Ruth Knudsen , Cinzia Tesauro , Yi-Ping Ho","doi":"10.1016/j.isci.2024.111332","DOIUrl":"10.1016/j.isci.2024.111332","url":null,"abstract":"<div><div>DNA-modifying enzymes are crucial in biological processes and have significant clinical implications. Traditional quantification methods often overlook enzymatic activity, the true determinants of enzymes’ functions. We present hydrogel Bead-based Isothermal Detection (BEAD-ID), utilizing uniform hydrogel bead-based microreactors to evaluate DNA-modifying enzyme activity on-bead. We fabricated homogeneous oligo-conjugated polyacrylamide (oligo-PAA) beads <em>via</em> droplet microfluidics, optimized for capturing and amplifying enzyme-modified nanosensors. By incorporating DNA oligos within the hydrogel network, BEAD-ID retains isothermally amplified products, facilitating <em>in situ</em> detection of enzyme activities on-bead. We validate BEAD-ID by quantifying human topoisomerase I (TOP1) and restriction endonuclease EcoRI, showing a direct correlation between enzyme concentration and fluorescence intensity, demonstrating the platform’s sensitivity (6.25 nM TOP1, 6.25 U/μL EcoRI) and reliability in food matrix (25 U/μL EcoRI). Additionally, a customized flow cytometry-mimicking setup allows high-throughput detection at 352 Hz with objective assessment. BEAD-ID, offering flexibility and scalability, is a promising tool for studying DNA-modifying enzymes.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111332"},"PeriodicalIF":4.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomic study of non-typeable Haemophilus influenzae in children with pneumonia and healthy controls 肺炎患儿和健康对照组非类型流感嗜血杆菌基因组比较研究

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-06 DOI: 10.1016/j.isci.2024.111330

Deming Zhang , Wenjian Wang , Chunli Song , Tingting Huang , Hongyu Chen , Zihao Liu , Yiwen Zhou , Heping Wang

{"title":"Comparative genomic study of non-typeable Haemophilus influenzae in children with pneumonia and healthy controls","authors":"Deming Zhang , Wenjian Wang , Chunli Song , Tingting Huang , Hongyu Chen , Zihao Liu , Yiwen Zhou , Heping Wang","doi":"10.1016/j.isci.2024.111330","DOIUrl":"10.1016/j.isci.2024.111330","url":null,"abstract":"<div><div>Non-typeable <em>Haemophilus influenzae</em> (NTHi) is a common pathogen causing respiratory infections, including pneumonia in children, and can also be found in the upper respiratory tracts of asymptomatic individuals. This study examines genomic variations between NTHi strains from healthy children and those from children with acute or chronic community-acquired pneumonia (CAP). Using bacterial genome-wide association studies (bGWAS), we compared these strains to identify key differences. Our analysis revealed that approximately 32% of genes exhibit variations between commensal and pathogenic states. Notably, we identified changes in <em>peptidoglycan biosynthesis</em> pathways and significant virulence factors associated with pneumonia. Furthermore, we observed a significant difference in β-lactam resistance due to PBP3 mutations between the healthy and pneumonia groups, confirmed by the ampicillin susceptibility test and characterized by the mutation pattern D350N, S357N, S385T, L389F. These findings contribute valuable insights into the genomic basis of NTHi pathogenicity and may inform more targeted clinical diagnostics and treatments.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111330"},"PeriodicalIF":4.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tracking fructose 1,6-bisphosphate dynamics in liver cancer cells using a fluorescent biosensor 利用荧光生物传感器跟踪肝癌细胞中 1,6-二磷酸果糖的动态变化

IF 4.6 2区综合性期刊

iScience Pub Date : 2024-11-06 DOI: 10.1016/j.isci.2024.111336

Israel Pérez-Chávez , John N. Koberstein , Julia Malo Pueyo , Eduardo H. Gilglioni , Didier Vertommen , Nicolas Baeyens , Daria Ezeriņa , Esteban N. Gurzov , Joris Messens

{"title":"Tracking fructose 1,6-bisphosphate dynamics in liver cancer cells using a fluorescent biosensor","authors":"Israel Pérez-Chávez , John N. Koberstein , Julia Malo Pueyo , Eduardo H. Gilglioni , Didier Vertommen , Nicolas Baeyens , Daria Ezeriņa , Esteban N. Gurzov , Joris Messens","doi":"10.1016/j.isci.2024.111336","DOIUrl":"10.1016/j.isci.2024.111336","url":null,"abstract":"<div><div>HYlight is a genetically encoded fluorescent biosensor that ratiometrically monitors fructose 1,6-bisphosphate (FBP), a key glycolytic metabolite. Given the role of glucose in liver cancer metabolism, we expressed HYlight in human liver cancer cells and primary mouse hepatocytes. Through <em>in vitro</em>, <em>in silico</em>, and <em>in cellulo</em> experiments, we showed HYlight’s ability to monitor FBP changes linked to glycolysis, not gluconeogenesis. HYlight’s affinity for FBP was ∼1 μM and stable within physiological pH range. HYlight demonstrated weak binding to dihydroxyacetone phosphate, and its ratiometric response was influenced by both ionic strength and phosphate. Therefore, simulating cytosolic conditions <em>in vitro</em> was necessary to establish a reliable correlation between HYlight’s cellular responses and FBP concentrations. FBP concentrations were found to be in the lower micromolar range, far lower than previous millimolar estimates. Altogether, this biosensor approach offers real-time monitoring of FBP concentrations at single-cell resolution, making it an invaluable tool for the understanding of cancer metabolism.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"27 12","pages":"Article 111336"},"PeriodicalIF":4.6,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0