iScience最新文献_第9页

Toward biomimetic optogenetics: Drug-free activation of acute opiate reward 走向仿生光遗传学：急性阿片奖赏的无药物激活

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-09-01 DOI: 10.1016/j.isci.2025.113465

Lyla El-Fayomi , Hendrik Steenland , Sabine Lovejoy , Michael Bergamini , Derek van der Kooy

{"title":"Toward biomimetic optogenetics: Drug-free activation of acute opiate reward","authors":"Lyla El-Fayomi , Hendrik Steenland , Sabine Lovejoy , Michael Bergamini , Derek van der Kooy","doi":"10.1016/j.isci.2025.113465","DOIUrl":"10.1016/j.isci.2025.113465","url":null,"abstract":"<div><div>Functional brain mapping studies typically involve tonic optogenetic stimulation. This does not always reflect natural firing patterns, which could be critical for information encoding. Adopting a biomimetic approach to optogenetics – wherein stimulation intervals mirror those observed <em>in vivo</em> to reflect context – we reconcile conflicting data on ventral tegmental area (VTA) GABA neurons in acute opiate reward. Activation using laser pulse sequences mimicking morphine-induced firing patterns is rewarding, while continuous light is aversive. When interspike intervals in rewarding morphine firing patterns are randomized, aversions also result, demonstrating the importance of temporal encoding in this system. We further establish the existence of VTA GABA projections to the tegmental pedunculopontine nucleus that drive reward in a dopamine-independent manner. Overall, our findings are consistent with non-optical studies characterizing VTA GABA neurons, and explain why previous optogenetic manipulations failed to corroborate those outcomes; proof-of-principle that temporal firing patterns are of critical importance in optogenetic toolkits.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113465"},"PeriodicalIF":4.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cerebellar white matter development is regulated by fractalkine-dependent microglia phagocytosis of oligodendrocyte progenitor cells 少突胶质细胞祖细胞的分形因子依赖性小胶质细胞吞噬调节小脑白质发育

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-09-01 DOI: 10.1016/j.isci.2025.113458

McKenzie K. Chappell , John Shelestak , Muhammad Irfan , Eric Shelestak , Ashley D. Nemes-Baran , Gabrielle M. Mey , Lakshmi Venkat , Amulya Surabhi , Tara M. DeSilva

{"title":"Cerebellar white matter development is regulated by fractalkine-dependent microglia phagocytosis of oligodendrocyte progenitor cells","authors":"McKenzie K. Chappell , John Shelestak , Muhammad Irfan , Eric Shelestak , Ashley D. Nemes-Baran , Gabrielle M. Mey , Lakshmi Venkat , Amulya Surabhi , Tara M. DeSilva","doi":"10.1016/j.isci.2025.113458","DOIUrl":"10.1016/j.isci.2025.113458","url":null,"abstract":"<div><div>Neurodevelopmental disorders often involve both motor and cognitive impairments, linked to the maturation of cerebral and cerebellar regions. This study reveals that during early postnatal development, ameboid microglia infiltrate the cerebellar white matter via the fourth ventricular zone, in parallel with similar activity in the cerebral white matter. These microglia phagocytose oligodendrocyte progenitor cells (OPCs) within a restricted time window before transitioning to a ramified state. The disruption of fractalkine receptor signaling—known to mediate synaptic pruning—reduces microglial OPC engulfment, leading to an increase in oligodendrocytes and altered myelin development. Variants in the fractalkine receptor gene have been linked to neurodevelopmental disorders such as autism and schizophrenia, both of which involve myelin abnormalities. These findings highlight a coordinated role of ameboid microglia in shaping white matter development in both the cerebrum and cerebellum, suggesting that disruptions in this process may contribute to altered brain circuitry and function in neurodevelopmental disorders.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113458"},"PeriodicalIF":4.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The voice of reflux: A systematic review about acoustic voice analysis in reflux patients 反流的声音：关于反流患者声学声音分析的系统综述

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-09-01 DOI: 10.1016/j.isci.2025.113468

Nina Moser , Yves Borbély

引用次数: 0

Current advances in diabetic neuropathy: Proteins as therapeutic targets 糖尿病神经病变的最新进展：蛋白质作为治疗靶点

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-09-01 DOI: 10.1016/j.isci.2025.113466

Gurjeet Kaur , Salaha Osman Ali , Alberto Santos , Tina Okdahl , Anne-Marie Wegeberg , Tarunveer S. Ahluwalia , Christian Stevns Hansen , David Wishart , Nicolai J. Wewer Albrechtsen , Birgitte Brock , Troels Staehelin Jensen , Peter Rossing , Christina Brock , Cristina Legido-Quigley , Karolina Sulek

{"title":"Current advances in diabetic neuropathy: Proteins as therapeutic targets","authors":"Gurjeet Kaur , Salaha Osman Ali , Alberto Santos , Tina Okdahl , Anne-Marie Wegeberg , Tarunveer S. Ahluwalia , Christian Stevns Hansen , David Wishart , Nicolai J. Wewer Albrechtsen , Birgitte Brock , Troels Staehelin Jensen , Peter Rossing , Christina Brock , Cristina Legido-Quigley , Karolina Sulek","doi":"10.1016/j.isci.2025.113466","DOIUrl":"10.1016/j.isci.2025.113466","url":null,"abstract":"<div><div>Diabetic neuropathy is a debilitating complication of diabetes characterized by nerve damage that may lead to numbness, foot ulcers, and amputations, and is a major cause of morbidity and mortality in people with diabetes. There are no treatments available for diabetic neuropathy aside from pain management. Recent advances in protein research have identified potential targets associated with the disease development and progression. This review explores the latest studies identifying proteins as possible drug targets in diabetic neuropathy and their role in relevant processes such as polyol metabolism, oxidative stress, and cytokine regulation. Additionally, we provide a comprehensive view of current developments and discoveries at single cell and spatial resolution that have revealed deregulated protein profiles in the dorsal root ganglia, sciatic nerve, trigeminal ganglion, or Schwann cells. Finally, we discuss the benefits of proteomics technologies to identify proteins and associated signaling pathways to better understand the source of diabetic neuropathy.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113466"},"PeriodicalIF":4.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MemorySeq identifies heritable epigenetic phenotypes that initiate cell culture stress tolerance in CHO cells MemorySeq鉴定了CHO细胞中启动细胞培养应激耐受性的可遗传表观遗传表型

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-09-01 DOI: 10.1016/j.isci.2025.113478

Spencer Grissom , Zachary Dixon , Abhyudai Singh , Mark Blenner

{"title":"MemorySeq identifies heritable epigenetic phenotypes that initiate cell culture stress tolerance in CHO cells","authors":"Spencer Grissom , Zachary Dixon , Abhyudai Singh , Mark Blenner","doi":"10.1016/j.isci.2025.113478","DOIUrl":"10.1016/j.isci.2025.113478","url":null,"abstract":"<div><div>During manufacturing batches, Chinese hamster ovary (CHO) cells encounter critical levels of environmental stressors which can significantly reduce cell health and productivity. Therefore, stress tolerance must be considered during selection of a suitable host. In this study, we employ a population-based transcriptomic method, referred to as MemorySeq, and differential gene expression analysis on stress-shocked CHO cells to identify stress responsive biomarkers. These biomarkers exhibit transient and intermediate heritable memory states characteristic of epigenetic switches and transcriptional bursting. Using this workflow, 199 genes were found to exhibit transcriptional variability characteristic of two-state systems with switching that forms four network communities of co-fluctuating genes. These communities were enriched in genes related to the regulation of apoptotic processes, gene expression, and metabolic pathways. Seven genes were identified as promising biomarkers of stress-resistance. Genetic engineering methods may be employed in the future to bias clonal populations toward higher stress tolerance to manufacturing stress.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113478"},"PeriodicalIF":4.1,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial artifact detection improves the reproducibility of drug screening experiments 空间伪影检测提高了药物筛选实验的再现性

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-30 DOI: 10.1016/j.isci.2025.113470

Aleksandr Ianevski , Kristen Nader , Swapnil Potdar , Alexandra Gorbonos , Filipp Ianevski , Ziaurrehman Tanoli , Jani Saarela , Tero Aittokallio

{"title":"Spatial artifact detection improves the reproducibility of drug screening experiments","authors":"Aleksandr Ianevski , Kristen Nader , Swapnil Potdar , Alexandra Gorbonos , Filipp Ianevski , Ziaurrehman Tanoli , Jani Saarela , Tero Aittokallio","doi":"10.1016/j.isci.2025.113470","DOIUrl":"10.1016/j.isci.2025.113470","url":null,"abstract":"<div><div>Reliable and reproducible drug screening experiments are essential for drug discovery and personalized medicine. We demonstrate how systematic experimental errors in drug plates negatively impact data reproducibility, and that conventional quality control (QC) methods based on plate controls fail to detect these spatial errors. To address this limitation, we developed a control-independent QC approach that uses normalized residual fit error (NRFE) to identify systematic artifacts in drug screening experiments. Analysis of >100,000 duplicate measurements from the PRISM pharmacogenomic study revealed that NRFE-flagged experiments show 3-fold lower reproducibility among technical replicates. By integrating NRFE with QC methods to analyze 41,762 matched drug-cell line pairs between two datasets from the Genomics of Drug Sensitivity in Cancer project, we improved the cross-dataset correlation from 0.66 to 0.76. Available as an R package at <span><span>https://github.com/IanevskiAleksandr/plateQC</span><svg><path></path></svg></span>, plateQC provides a robust toolset for enhancing drug screening data reliability and consistency for basic research and translational applications.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113470"},"PeriodicalIF":4.1,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synaptic pruning following NMDAR-dependent LTD preferentially affects isolated synapses nmdar依赖性LTD后的突触修剪优先影响孤立突触

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-29 DOI: 10.1016/j.isci.2025.113093

Côme Camus , Léa Leval , Viviana Villicana-Munoz , Sarka Jelinkova , Benjamin Compans , Frédéric Gambino , Etienne Herzog , Daniel Choquet , Eric Hosy

引用次数: 0

Investigating the fire dynamics of mounted PV weathering effects and material changes. 研究安装PV的火灾动力学，风化效应和材料变化。

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-29 eCollection Date: 2025-09-19 DOI: 10.1016/j.isci.2025.113410

Muhsin Mohamed Baseer Ahamed Mohamed, Ye Xian Ang, Rhonda Jia Hui Tan, Li Song Tung, Xingchi Xiao, Maloy Das, Leonard Wei Tat Ng

{"title":"Investigating the fire dynamics of mounted PV weathering effects and material changes.","authors":"Muhsin Mohamed Baseer Ahamed Mohamed, Ye Xian Ang, Rhonda Jia Hui Tan, Li Song Tung, Xingchi Xiao, Maloy Das, Leonard Wei Tat Ng","doi":"10.1016/j.isci.2025.113410","DOIUrl":"10.1016/j.isci.2025.113410","url":null,"abstract":"<p><p>Solar photovoltaic (PV) systems constitute approximately 37% of global renewable energy capacity, yet their fire safety under environmental degradation remains inadequately understood. PV backsheets serve as the primary interface between external fire sources and modules, making their long-term fire performance critical for system safety. This study systematically quantified flame spread behavior on weathered PV backsheets. Two commercial backsheet types underwent accelerated weathering for up to six weeks, followed by comprehensive characterization using Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and tensile testing. Fire performance was assessed using a time-to-marker (TTM) methodology to measure flame propagation rates. Six-week weathered samples exhibited a 46% faster flame spread, demonstrating significant degradation in fire resistance. Chemical analysis revealed polymer chain scission and formation of degradation products, while mechanical testing showed up to 18% reduction in tensile strength. These findings highlight critical gaps in current safety standards and demonstrate the importance of incorporating weathering effects into PV fire safety assessments for long-term system reliability.</p>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 9","pages":"113410"},"PeriodicalIF":4.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12447895/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance and challenges of power sector reform in China since 2015 2015年以来中国电力行业改革的绩效与挑战

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113461

Yanbo Feng , Jiang Lin , Feng Song , Mun Sing Ho

{"title":"Performance and challenges of power sector reform in China since 2015","authors":"Yanbo Feng , Jiang Lin , Feng Song , Mun Sing Ho","doi":"10.1016/j.isci.2025.113461","DOIUrl":"10.1016/j.isci.2025.113461","url":null,"abstract":"<div><div>China has launched a comprehensive market-oriented reform of the power sector since 2015 to foster competition and enhance efficiency. This article summarizes the progress, achievements, and challenges of these reforms. Progress has been made in enhancing competition in wholesale and retail markets, strengthening regulation over grid costs and services, expanding inter-regional transmission, and promoting renewable electricity. Reform efforts have yielded several notable achievements: significantly improving generation efficiency, particularly among smaller-capacity units in the short term; substantially reducing industrial and commercial electricity prices, though primarily due to government intervention rather than market competition; effectively capturing market dynamics through spot market, although their limited scale has constrained broader impacts; and improving efficiency and welfare through market integration but raising concerns about equity. In the transition to a low-carbon energy future, two pivotal challenges remain: striking a balance between market mechanisms and government intervention; and ensuring system flexibility and long-term resource adequacy.</div></div>","PeriodicalId":342,"journal":{"name":"iScience","volume":"28 10","pages":"Article 113461"},"PeriodicalIF":4.1,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Broadband circularly polarized magneto-electric dipole antenna for millimeter-wave communication 毫米波通信用宽带圆极化磁电偶极天线

IF 4.1 2区综合性期刊

iScience Pub Date : 2025-08-28 DOI: 10.1016/j.isci.2025.113469

Ting Wu , Chen Zhang

引用次数: 0