Cellular and synaptic properties of molecularly defined neurons in the external globus pallidus

IF 4.1 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-06-27 DOI:10.1016/j.isci.2025.113002

Linda M.C. Koene , Wilhelm Thunberg , Sten Grillner , Gilad Silberberg , Maya Ketzef

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Abstract

The external globus pallidus (GPe) is a central basal ganglia (BG) nucleus, involved in various sensorimotor functions. The two main types of GPe neurons, the prototypic and arkypallidal neurons, differ in developmental origin, axonal projections, and functional roles. Using ex vivo whole-cell patch-clamp recordings, we characterized the membrane properties, morphology, and synaptic connectivity of molecularly identified GPe neurons. Arkypallidal neurons had broader, slower action potentials with higher thresholds, and smaller somata, dendritic length, and surface area than prototypic neurons. Optogenetic activation of prototypic neurons inhibited both GPe subpopulations, but paired recordings revealed very sparse direct synaptic connectivity only between prototypic neurons. Moreover, parvalbumin-expressing prototypic neurons were not electrically coupled. This work provides a comprehensive description of the GPe microcircuitry and may provide insight into the functional role of the GPe within the BG network.

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外苍白球分子定义神经元的细胞和突触特性

外苍白球（GPe）是中央基底神经节（BG）核，参与多种感觉运动功能。GPe神经元的两种主要类型，即原型神经元和树状神经元，在发育起源、轴突投射和功能作用方面存在差异。利用离体全细胞膜片钳记录，我们表征了分子鉴定的GPe神经元的膜特性、形态和突触连通性。与原型神经元相比，树泡神经元具有更宽、更慢、更高阈值的动作电位，以及更小的体、树突长度和表面积。光遗传激活的原型神经元抑制两个GPe亚群，但配对记录显示，只有原型神经元之间的直接突触连接非常稀疏。此外，表达小蛋白的原型神经元没有电偶联。这项工作提供了GPe微电路的全面描述，并可能提供洞察GPe在BG网络中的功能作用。

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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