Biosensors and Bioelectronics: X最新文献

{"title":"Green nanotechnology for implantable biosensors: Biocompatibility and functional integration in medical applications","authors":"Amran Hossain ,&nbsp;Mehedi Hasan Manik ,&nbsp;Saifuddin Rakib ,&nbsp;Naym Mahmud ,&nbsp;Safiullah Khan ,&nbsp;Zahid Ahsan ,&nbsp;Md Safiul Islam ,&nbsp;Nayem Hossain ,&nbsp;Mosst Asma Akter","doi":"10.1016/j.biosx.2025.100678","DOIUrl":"10.1016/j.biosx.2025.100678","url":null,"abstract":"<div><div>Green nanotechnology is increasingly leveraged to create biocompatible, environmentally friendly implanted biosensors that transform medical diagnostics without the ecological costs associated with conventional fabrication. Utilizing in-situ phytochemicals or microbial enzymes in plant extract, microbe, and biopolymer synthesis methods enables environmentally responsible nanoparticle synthesis of Graphene, Carbon Nanotubes (CNTs), Gold Nanoparticles (AuNPs), Silver Nanoparticles (AgNPs) and Quantum Dots(QDs) with greater cell viability and colloidal stability compared to those synthesized using the citrate reduction method. The functional integration of green-synthesized nanomaterials into biosensors enables nanomaterials to perform precise detection of biomarkers, such as glucose, lactate, and proteins, with high sensitivity, specificity, and signal transduction, for point-of-care applications and personalized medicine. Convergence of Internet of Things (IoT) integration in intelligent sensing networks that bridge biomedical diagnostics and environmental parameter monitoring, safety for chronic disease management, while minimizing contact, enhances the reliability of data and minimizes energy usage. Regulatory hurdles and critical challenges in translating from in vitro to in vivo applications, including surgical implantation risks, calibration drift, and chronic biocompatibility issues. Biodegradable electronics, AI-assisted analytics, and automated stimuli-responsive nanomaterials that adjust to physiological changes are highlighted as future directions. Bioresorbable sensors and self-healing polymers are examples of innovations that highlight the move toward patient-centered, sustainable healthcare. Green nanotechnology opens the door to implanted biosensors that balance environmental responsibility with state-of-the-art medical innovation by linking the fields of material science, bioengineering, and clinical practice. To overcome current obstacles and realize the full potential of implanted biosensors in precision medicine, this study emphasizes the need to develop green approaches.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100678"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PNA-based biosensors for portable electrochemical diagnostics: Integration strategies and probe design","authors":"Younghwan Kim ,&nbsp;Swomitra Kumar Mohanty","doi":"10.1016/j.biosx.2025.100702","DOIUrl":"10.1016/j.biosx.2025.100702","url":null,"abstract":"<div><div>Portable and mobile biosensors are widely applied in point-of-care (POC) diagnostics due to their rapid response and suitability for decentralized testing. While DNA-based probes have been commonly used in electrochemical biosensors owing to their label-free operation, low cost, and sensitivity, their susceptibility to enzymatic degradation and low thermal stability has limited their broader utility. As an alternative, peptide nucleic acid (PNA) probes have gained increasing attention due to their synthetic backbone, which is based on an electrically neutral N-(2-aminoethyl)glycine (AEG) structure. This neutrality confers high resistance to nucleases and polymerases, while enabling stronger and more stable hybridization with negatively charged DNA targets. PNA probes also maintain structural integrity under low ionic strength conditions, improving signal stability compared to conventional DNA probes. These advantages have led to a growing body of research exploring PNA-based biosensors for portable applications. This mini-review surveys recent developments in this area, focusing on the physicochemical properties of PNA and their implications for biosensor design. Future directions for PNA biosensor development are also discussed. Representative studies on electrode architectures and detection strategies are highlighted, demonstrating the potential of PNA-based systems to support next-generation portable diagnostics.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100702"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145358739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart underwear: A novel wearable for long-term monitoring of gut microbial gas production via flatus","authors":"Santiago Botasini ,&nbsp;David Zhan ,&nbsp;Norman Fischer ,&nbsp;Charlotte T. Ravel ,&nbsp;Ashley Tien ,&nbsp;Maggie R. Grant ,&nbsp;Glory Minabou Ndjite ,&nbsp;Ty Sopko ,&nbsp;Holly Childs ,&nbsp;Maryann Greenfield ,&nbsp;Christina X. Qian ,&nbsp;Kara E. Gardiner ,&nbsp;Nayantara M. Anders ,&nbsp;Tasnim F. Ullah ,&nbsp;Leah T. Redmond ,&nbsp;Delaina A. Callaway ,&nbsp;Eliya M. Behailu ,&nbsp;Grace M. Sarkar ,&nbsp;Nakati C. Sany ,&nbsp;Margaret Slavin ,&nbsp;Brantley Hall","doi":"10.1016/j.biosx.2025.100699","DOIUrl":"10.1016/j.biosx.2025.100699","url":null,"abstract":"<div><div>The temporal dynamics of gut microbial metabolism, including how quickly and variably microbes respond to dietary changes, remain largely invisible to current research methods. While measuring metabolic outputs like short-chain fatty acids (SCFAs) in stool or blood provide insights into microbial metabolism, sampling frequency is severely limited, preventing observation of the temporal dynamics of how gut microbes respond to dietary inputs and environmental changes on an hour-by-hour basis. Hydrogen gas, produced by gut microbial metabolism, represents an underutilized biomarker for continuous monitoring of microbial metabolic activity. Following its production, hydrogen is expelled through two major routes: breath and flatus, with flatus containing dramatically higher hydrogen concentrations compared to breath, making it an ideal target for sensitive detection. Currently, no tools exist for continuous, non-invasive monitoring of hydrogen in flatus to track changes in gut microbial metabolism. To address this gap, we developed the Smart Underwear, the first wearable device that continuously measures hydrogen gas expelled in flatus, providing unprecedented temporal resolution of gut microbial activity. In a week-long User Experience Study, the Smart Underwear successfully tracked hydrogen in flatus with participants reporting high comfort and compliance. In a second study, the Smart Underwear accurately detected increased hydrogen production following inulin ingestion with 94.7 % sensitivity in 38 healthy participants, demonstrating its capability to track diet-induced changes in microbial metabolism. This novel platform enables longitudinal studies of microbiome function, capturing both inter-individual differences and intra-individual temporal dynamics, while opening new avenues for investigating diet-microbiome interactions, circadian patterns, and the functional consequences of changes in microbial composition.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100699"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145321016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Flexible gold nanoparticle SERS tape for rapid, label-free and ultrasensitive detection and differentiation of Shiga toxin variants” [Biosens. Bioelectron. X 27 (2025) 100696]","authors":"A. D'Avino ,&nbsp;A. Milano ,&nbsp;V. Marchesano ,&nbsp;B. Guilcapi ,&nbsp;D. Sagnelli ,&nbsp;M. Rippa ,&nbsp;L. Zhou ,&nbsp;G. Rossi ,&nbsp;L. Consagra ,&nbsp;M. Brigotti ,&nbsp;S. Morabito ,&nbsp;L. Petti","doi":"10.1016/j.biosx.2025.100704","DOIUrl":"10.1016/j.biosx.2025.100704","url":null,"abstract":"","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100704"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145690496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cas12b-assisted one-step dual-target CRISPR system for Mycobacterium tuberculosis detection","authors":"Xike Zhou ,&nbsp;Xuchun Han ,&nbsp;Lifang Liu ,&nbsp;Xiaoping Wang ,&nbsp;Junjie Lu ,&nbsp;Long Qiu ,&nbsp;Tao Wu ,&nbsp;Hao Pei ,&nbsp;Guping Zhao ,&nbsp;Jin Wang","doi":"10.1016/j.biosx.2025.100692","DOIUrl":"10.1016/j.biosx.2025.100692","url":null,"abstract":"<div><div>Tuberculosis (TB) remains a persistent public health threat, while the efficiency of traditional diagnostic methods is restricted. To address this, we developed a CRISPR-Cas12b-based one-step dual-target detection system (TB-QUICKv2) for sensitive and specific detection of the <em>Mycobacterium tuberculosis</em> complex (MTBC). Specifically, Cas12b and a molecular beacon (MB) probe were used to detect the target MTBC IS6110 sequence and an internal control (IC), the <em>Bacillus spizizenii</em> 16S sequence, in one reaction system, respectively. The optimal reaction temperature for TB-QUICKv2 was 57 °C and the detection procedure can be completed within 25 min. TB-QUICKv2 is highly specific (100 %) for MTBC with a limit of detection of 10.4 CFU per milliliter at a 95 % confidence interval. TB-QUICKv2 was further validated with clinical sputum samples and the results showed remarkably higher sensitivity (41/74) than the traditional culture method (37/74) and acid-fast bacillus (AFB) testing (33/74). Therefore, this study demonstrates that TB-QUICKv2 is a promising method for the rapid and accurate detection of MTBC and other infectious pathogens in the future.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100692"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paper-based origami assisted and enhanced electroanalytical detection of β-Amyloid peptide in plasma samples","authors":"Antonella Miglione ,&nbsp;Panagiota M. Kalligosfyri ,&nbsp;Claudia Corbo ,&nbsp;Donato Calabria ,&nbsp;Mara Mirasoli ,&nbsp;Marco Frasconi ,&nbsp;Stefano Cinti","doi":"10.1016/j.biosx.2025.100687","DOIUrl":"10.1016/j.biosx.2025.100687","url":null,"abstract":"<div><div>In this work, we demonstrate how the analytical performance towards β-amyloid (Aβ(1–42) detection can be easily improved by using a printed label-free electrochemical immunosensor coupled to an external paper-based origami. Coupling the immunosensor with a customized foldable origami allows for an increase of resulting sensitivity towards the detection of Aβ(1–42) in both standard and biological samples. The features of paper are fundamental for the analysis of Aβ(1–42), recognized as a central indicator of Alzheimer's pathology, considering the low levels in plasma associated with early-stage disease. The proposed strategy highlights up to a 5-fold signal enhancement in both buffer and plasma at low Aβ(1–42) concentrations, bringing the platform's performance toward clinical requirements. The immunoplatform has been systematically optimized through electrochemical characterization and parameter tuning, achieving limits of detection of 2.7 ng/mL in buffer and 4.7 ng/mL in undiluted plasma, with a linear range up to 1 μg/mL. The platform shows satisfactory repeatability (&lt;10 %) and excellent selectivity in the presence of interfering plasma proteins. Overall, this work offers a promising foundation for the development of accessible and scalable tools for early Alzheimer's disease screening.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100687"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145155681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding point-of-care testing: A strategic tool for influenza and SARS-CoV-2 management in healthcare facilities","authors":"Caterina Ledda ,&nbsp;Helena C. Maltezou ,&nbsp;Gaetano Isola ,&nbsp;Giuseppe Motta ,&nbsp;Carmelina Daniela Anfuso ,&nbsp;Claudio Costantino ,&nbsp;Venerando Rapisarda","doi":"10.1016/j.biosx.2025.100691","DOIUrl":"10.1016/j.biosx.2025.100691","url":null,"abstract":"<div><div>The emergence of SARS-CoV-2 and the seasonal burden of influenza have underscored the urgent need for rapid and decentralized diagnostic tools in healthcare settings. This review explores the role of Point-of-Care (POC) testing in controlling respiratory virus transmission, focusing on its technical foundations, clinical applications, and implementation challenges. A systematic literature screening was conducted across PubMed, Scopus, and Web of Science, prioritizing studies from 2020 onward, to examine the diagnostic performance, use cases, and operational constraints of rapid antigen and molecular POC platforms. Comparative analysis highlights substantial differences in analytical sensitivity, turnaround times, cost-effectiveness, and deployment logistics between isothermal amplification, RT-PCR-based, and immunochromatographic assays. Emerging technologies, including SERS-based biosensors and multiplex portable platforms, are also discussed for their potential to enhance specificity and scalability. A dedicated section addresses real-world barriers such as device cost, personnel training, sample throughput, and regulatory considerations. Figure-based summaries and performance tables are included to assist clinicians and policymakers in selecting appropriate tools. Finally, we propose a strategic framework for integrating POC diagnostics into infection control protocols in hospitals and occupational health services. The findings suggest that when appropriately selected and deployed, POC testing can significantly reduce transmission risk, optimize patient management, and minimize workforce disruption. These outcomes justify broader adoption of POC tools and continued investment in innovation and clinical validation.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100691"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and fabrication of an electrochemical nano-biosensor for the quick sensing of SARS CoV-2","authors":"Sonali Priyadarshini ,&nbsp;Kanchan Karki ,&nbsp;Sanjay Kumar ,&nbsp;Lakshika Bhandari ,&nbsp;Krishna Pal Singh ,&nbsp;Narotam Sharma ,&nbsp;Anuj Nehra","doi":"10.1016/j.biosx.2025.100695","DOIUrl":"10.1016/j.biosx.2025.100695","url":null,"abstract":"<div><div>Graphene oxide (GO) emerged as a biosensing material due to its patternable features, electron transfer properties, high functionality, and greater surface area that enables sensitive point-of-use applications. This report outlines the design of an electrochemical biosensor composed of a polycarbonate track-etched (PCTE) nano-sieve platform with two silver electrodes, which can detect the surface glycoprotein of the severe acute respiratory syndrome coronavirus 2 (SARS CoV-2), specifically the Spike protein (S-protein), in a rapid and sensitive manner. To fabricate the GO material, we utilized the modified Hummers' method to convert graphite powder. In this study, we covalently immobilized SARS-CoV-2 specific antibodies onto an EDC-NHS functionalized nanosieve platform using two methods i.e., conventional or traditional method and protein-G mediated. The immobilization of these antibodies on the nanosieve platform was achieved through bio-linkage, resulting in specific interactions between the spike protein and the antibodies. These interactions led to a partial blockage of the nanosieve, this led to a considerable decrease in the ionic current for a voltage range of 1.0–2.0 V. The linear range was set between 3.6 mM and 3.6 aM. The detection limit was in nM in traditional method which was notably decreased to fM in protein-G mediated antibodies immobilization. Notably, when testing two non-specific proteins, bovine serum albumin (BSA) and influenza virus, under the same settings, there was no significant change in the current. This nano-biorecognition platform, or nanobiosensor, offers improved stability and higher sensitivity due to the integration of minor GO laminates.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100695"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a highly sensitive SPR biosensor for rapid malaria detection based on zinc telluride and blue phosphorus/tungsten disulfide material","authors":"Md. Al Amin Islam Utshob,&nbsp;Maymona Binte Juwel,&nbsp;Rawfarin Sabiha,&nbsp;Aminur Rahman,&nbsp;Safayat-Al Imam,&nbsp;Khandakar Mohammad Ishtiak","doi":"10.1016/j.biosx.2025.100709","DOIUrl":"10.1016/j.biosx.2025.100709","url":null,"abstract":"<div><div>Malaria continues to be a prominent global health issue, particularly in regions facing drug-resistant Plasmodium strains and climate-induced transmission expansion. To address the need for rapid and sensitive diagnostic devices, this research proposes an innovative surface plasmon resonance (SPR) biosensor design based on a BK7-ZnTe-Ag-Fe₂O₃-BlueP/WS₂ multilayer structure. To analyze the proposed SPR structure, both the finite element method (FEM) and the transfer matrix method (TMM) employed. The optimal architecture utilizes ZnTe (12 nm) for plasmonic coupling and light confinement, Ag (40 nm) for enhanced plasmonic sensitivity, Fe₂O₃ (3 nm) for interaction with the surface and stability and a BlueP/WS₂ monolayer for enhanced evanescent field and signal response. This proposed biosensor possesses maximum angular sensitivity of 444 (Degree/RIU), FWHM of 4.63 (Degree), figure of merit (FOM) of 95.80 and quality factor (QF) of 95.83 (1/RIU), with particularly good detection of the ring stage of malaria infection. Comparative studies with other sensor configurations reveal considerable enhancement in sensitivity and detection accuracy. High linearity of performance parameters with refractive index changes verifies the strength and reliability of the sensor for real-time malaria detection. The optimized SPR biosensor represents a low-cost, scalable platform for malaria detection at an early stage and can be extended to other biomedical applications, particularly in resource-limited settings.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100709"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feasibility study of a smart insole with triboelectric energy harvesters for early flatfoot detection","authors":"Mohammad Alghamaz,&nbsp;Leila Donyaparastlivari,&nbsp;Alwathiqbellah Ibrahim,&nbsp;Nelson Fumo","doi":"10.1016/j.biosx.2025.100649","DOIUrl":"10.1016/j.biosx.2025.100649","url":null,"abstract":"<div><div>This study introduces a self-powered smart insole system designed for real-time monitoring of foot health, with a specific focus on detecting flatfoot conditions. The insole integrates multiple identical triboelectric energy harvesters strategically positioned to capture electrical signals generated from ground reaction forces during daily activities such as walking, jogging, and running. Proof-of-concept testing was conducted on a single participant under two conditions: a healthy foot and a simulated flatfoot created by reducing the medial arch height by approximately 70%. In the healthy foot trials, the system demonstrated consistent and reliable performance, with negligible electrical output from the medial arch sensor due to minimal ground contact in this region. In contrast, the simulated flatfoot condition produced a significant increase in voltage output from the medial arch sensor, successfully identifying the abnormal foot mechanics associated with arch collapse. Additionally, a neural network was implemented to classify healthy and flatfoot conditions from the collected data, achieving an accuracy of 86% and a precision of 96%, demonstrating the feasibility of machine learning integration for automated flatfoot detection. Overall, the findings validate the smart insole’s capability as a promising tool for continuous foot health monitoring, early diagnosis of flatfoot, and future applications in personalized rehabilitation and preventative care.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100649"},"PeriodicalIF":10.61,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144580058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}