Biosensors and Bioelectronics: X最新文献_第6页

Rational design of peptides for epitope imprinting of polynorepinephrine: A plasmonic and machine learning integrated approach 多去甲肾上腺素表位印迹肽的合理设计：等离子体和机器学习的综合方法

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-06-06 DOI: 10.1016/j.biosx.2025.100638

Davide Sestaioni , Giulia Ciacci , Andrea Barucci , Pasquale Palladino , Simona Scarano

{"title":"Rational design of peptides for epitope imprinting of polynorepinephrine: A plasmonic and machine learning integrated approach","authors":"Davide Sestaioni , Giulia Ciacci , Andrea Barucci , Pasquale Palladino , Simona Scarano","doi":"10.1016/j.biosx.2025.100638","DOIUrl":"10.1016/j.biosx.2025.100638","url":null,"abstract":"<div><div>Molecularly Imprinted Polynorepinephrine (MIPNE) has demonstrated superior performance for mimetic receptors production, facilitating their integration into techniques like Surface Plasmon Resonance (SPR), Biomimetic Enzyme-Linked ImmunoSorbent Assay (BELISA), and Bio-Layer Interferometry (BLI). Here we developed a multiplexed Localized Surface Plasmon Resonance (LSPR) assay to face the selection of appropriate epitope sequences for protein imprinting, a critical factor in optimizing MIPNE efficiency. The plasmonic properties of gold nanoparticles formed on MIPNE were used to classify epitopes as functional (<strong><em>F</em></strong>), uncertain (<strong><em>U</em></strong>), or dysfunctional (<strong><em>D</em></strong>). Feature extraction and machine learning analysis identified key physico-chemical descriptors influencing imprinting efficiency. Subsequent SPR testing confirmed the correlation between epitope selection and receptor performance. This study provides the first systematic approach for epitope selection in MIPNE, paving the way for their improved design and application in bioanalytics and biosensing.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100638"},"PeriodicalIF":10.61,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Portable cotton swab biosensor for rapid naked-eye detection of Helicobacter pylori 用于幽门螺杆菌快速肉眼检测的便携式棉签生物传感器

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-05-24 DOI: 10.1016/j.biosx.2025.100636

Ghadeer.A.R.Y. Suaifan , Asmaa Alnajajrah , Ward Abu Jbara , Amr A. El-Mousa , Fahid Abu Jbara , Doha A.I. Al-Omari , Mayadah B. Shehadeh

{"title":"Portable cotton swab biosensor for rapid naked-eye detection of Helicobacter pylori","authors":"Ghadeer.A.R.Y. Suaifan , Asmaa Alnajajrah , Ward Abu Jbara , Amr A. El-Mousa , Fahid Abu Jbara , Doha A.I. Al-Omari , Mayadah B. Shehadeh","doi":"10.1016/j.biosx.2025.100636","DOIUrl":"10.1016/j.biosx.2025.100636","url":null,"abstract":"<div><div>To better understand the oral cavity's role as a potential reservoir and transmission route for <em>Helicobacter pylori</em> (<em>H. pylori</em>), the development of a diagnostic test that is not only rapid and highly specific but also user-friendly is essential. In response to this need, a colorimetric cotton swab biosensor was designed to enable visual detection of <em>H. pylori</em> presence or absence. This study presents the development of a rapid, simple and cost-effective cotton swab-based colorimetric biosensor for the visual detection of <em>H. pylori</em> in saliva. The biosensor utilizes a lactoferrin-functionalized cotton platform for bacterial capture, followed by signal generation using polymeric nanobeads conjugated to specific anti-<em>H. pylori</em> antibodies. The assay enables direct, naked-eye detection without the need for amplification steps or specialized instrumentation. A visual limit of detection between 10 CFU/mL was achieved within 5 min in solution and in spiked saliva samples, offering both qualitative and semi-quantitative results. The biosensor exhibited high specificity, showing no cross-reactivity with <em>E. coli</em> or <em>S. aureus</em>, and maintained analytical performance despite the presence of mucin in Saliva. Moreover, the device demonstrated operational stability over extended storage periods. These findings support the biosensor's utility as a point-of-care diagnostic tool in low-resource settings for the early detection and surveillance of <em>H. pylori</em> infections.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100636"},"PeriodicalIF":10.61,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144205160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D-printed smartphone-based platform for in-situ and rapid monitoring of aquaculture pathogens using polydimethylsiloxane (PDMS) microchip with multiplex loop-mediated isothermal amplification (M-LAMP) 基于智能手机的3d打印水产养殖病原体原位和快速监测平台——多环介导等温扩增（M-LAMP）聚二甲基硅氧烷（PDMS）微芯片

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-05-15 DOI: 10.1016/j.biosx.2025.100635

Liyan Li , Jing Fu , Elaine Li Ching Chiang , Jerald Yoo , Sungwoo Bae

{"title":"A 3D-printed smartphone-based platform for in-situ and rapid monitoring of aquaculture pathogens using polydimethylsiloxane (PDMS) microchip with multiplex loop-mediated isothermal amplification (M-LAMP)","authors":"Liyan Li , Jing Fu , Elaine Li Ching Chiang , Jerald Yoo , Sungwoo Bae","doi":"10.1016/j.biosx.2025.100635","DOIUrl":"10.1016/j.biosx.2025.100635","url":null,"abstract":"<div><div>Aquaculture pathogens pose serious risks to aquatic livestock and global food safety. Key threats in shrimp farming include white spot syndrome virus (WSSV), <em>Vibrio parahaemolyticus</em> (causing acute hepatopancreatic necrosis disease, AHPND), and <em>Enterocytozoon hepatopenaei</em> (EHP). Rapid, on-site detection is critical for early detection and outbreak prevention. In this study, we developed a portable, smartphone-based diagnostic platform utilizing multiplex loop-mediated isothermal amplification (LAMP) for simultaneous detection of multiple pathogens in a single reaction. A PDMS microchip with 30 reaction wells (5 × 6 array) and a temperature control well was fabricated for efficient multiplexing. Immobilized LAMP reagents with freeze-drying lyophilization were preloaded into wells to streamline preparation and enhance stability. The system successfully identified both waterborne indicator bacteria (<em>E. coli</em>, <em>E. faecalis</em>, <em>Salmonella</em>) and major shrimp pathogens (WSSV, AHPND, EHP) in samples from <em>Penaeus vannamei</em>, <em>Penaeus monodon</em>, and aquaculture water. The microchip maintained stable isothermal conditions (65.1 ± 0.6 °C), enabling visual detection via color change at low DNA concentrations (as low as 4 copies/μL). All WSSV and EHP infections in shrimp tissues and water samples were correctly identified using LAMP reaction within 35 min (excluding the DNA extraction process), demonstrating 100% positive detection rates. The smartphone interface allowed real-time imaging and result interpretation, offering a rapid, user-friendly tool for in situ pathogen monitoring. This platform represents a practical, low-cost solution for field diagnostics and improved disease management in aquaculture.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100635"},"PeriodicalIF":10.61,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144099714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanocrystal electrochemiluminescence Biosensor: Paving the way from lab discovery to market innovation 纳米晶体电化学发光生物传感器：从实验室发现到市场创新的道路

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-05-08 DOI: 10.1016/j.biosx.2025.100633

Abhishek Kumar , Sanket Goel

{"title":"Nanocrystal electrochemiluminescence Biosensor: Paving the way from lab discovery to market innovation","authors":"Abhishek Kumar , Sanket Goel","doi":"10.1016/j.biosx.2025.100633","DOIUrl":"10.1016/j.biosx.2025.100633","url":null,"abstract":"<div><div>The commercial viability of a biosensor depends on its capability to accurately and precisely perform detection of target molecules in real physiological body fluids such as whole blood, serum, plasma, urine, saliva, tears, or sweat. A biosensor is considered ideal for detecting molecules if enriched with specific characteristics crucial for accurate outputs, namely low detection limit, high sensitivity, selectivity to target analyte, reproducibility and repeatability, and performance in real samples. Electrochemiluminescence (ECL) is a strong analytical technique with major applications in biosensing due to its inherent features from electrochemistry and photoluminescence. While existing ECL biosensors can deliver satisfactory performance in laboratory settings, only a limited number are effective in real complex matrices. The stability of biosensors in real samples is a significant concern, which often limits their commercial applications. Incorporation of nanomaterials in ECL biosensors has transformed the biomolecule detection process by providing unparalleled selectivity and sensitivity. This article deliberates on rendering contributions of nanomaterials in advancing traditional ECL biosensors to pave the way from lab discovery to market innovation. Furthermore, the article highlights the various roles of nanomaterials in addressing the critical challenges associated with the commercialization of ECL biosensors. Moreover, various essential concepts are highlighted with relevant figures and comparative tables to provide a general overview of the nanomaterial based ECL biosensors. Lastly, the future outlook and prospects of ECL biosensors in advancing molecular and clinical diagnostics is discussed.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100633"},"PeriodicalIF":10.61,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing food Safety: Two-plex electrochemical biosensor for mycotoxin detection in food matrices 推进食品安全：用于食品基质中霉菌毒素检测的双路电化学生物传感器

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-05-06 DOI: 10.1016/j.biosx.2025.100626

Kundan Kumar Mishra , Vikram Narayanan Dhamu , Abhinav Kokala , Sriram Muthukumar , Shalini Prasad

{"title":"Advancing food Safety: Two-plex electrochemical biosensor for mycotoxin detection in food matrices","authors":"Kundan Kumar Mishra , Vikram Narayanan Dhamu , Abhinav Kokala , Sriram Muthukumar , Shalini Prasad","doi":"10.1016/j.biosx.2025.100626","DOIUrl":"10.1016/j.biosx.2025.100626","url":null,"abstract":"<div><div>Detecting foodborne toxins like Aflatoxin B1 and Zearalenone remains a pressing global health concern due to their impact on food safety. Conventional detection techniques lack the required sensitivity and efficiency, showcasing the urgent need for advanced, rapid detection solutions. This study presents a portable, non-faradaic electrochemical sensing platform specifically designed for quick and accurate detection of these toxins in corn flour. Featuring a short assay time of 5 min, the 2-plex platform leverages antibodies specific to Aflatoxin B1 and Zearalenone, achieving detection limits of 0.005 ng/mL and 0.05 ng/mL, respectively. The system offers a dynamic detection range of 0.01–9.151 ng/mL for Aflatoxin B1 and 0.1–25.6 ng/mL for Zearalenone. The platform demonstrates consistent performance, maintaining inter- and intra-study coefficient of variation (%CV) below 20 %. Validation against benchtop and outsource laboratory data confirms its real-world applicability. This user-friendly device holds promise for on-site toxin detection, enhancing food safety and public health.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100626"},"PeriodicalIF":10.61,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection 镍掺杂壳聚糖纳米颗粒作为胰岛素电化学检测新平台的合成

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-05-03 DOI: 10.1016/j.biosx.2025.100624

Frederika Chovancová , Marjan Motiei , Ivana Šišoláková , Michal Urbánek , Jana Shepa , Haojie Fei , Petr Sáha , Renáta Oriňaková

{"title":"Synthesis of nickel dopped chitosan nanoparticles as a novel platform for electrochemical insulin detection","authors":"Frederika Chovancová , Marjan Motiei , Ivana Šišoláková , Michal Urbánek , Jana Shepa , Haojie Fei , Petr Sáha , Renáta Oriňaková","doi":"10.1016/j.biosx.2025.100624","DOIUrl":"10.1016/j.biosx.2025.100624","url":null,"abstract":"<div><div>Nickel modified chitosan nanoparticles are promising catalysts for the determination of bioanalytes such as insulin, glucose, antibiotics, and ascorbic acid. In this study, we synthesized nickel-loaded chitosan nanoparticles to evaluate their potential as surface modifiers for electrochemical sensors for insulin detection. The nanoparticles were prepared using the ionic gelation of chitosan with tripolyphosphate anions, followed by adsorption of nickel ions via ion-exchange resins and surface chelation. The physicochemical properties of the nanoparticles were characterized by scanning electron microscopy with EDX analysis, transmission electron microscopy, Fourier-transform infrared spectroscopy, and dynamic light scattering. The catalytic activity of nickel modified chitosan nanoparticles towards insulin oxidation was investigated through cyclic voltammetry. The resulting screen-printed carbon electrode modified with nickel-chitosan nanoparticles exhibited exceptional analytical performance, including high sensitivity (0.09 mA μM), a low detection limit (0.02 μM), and a wide dynamic range (300 nM–5 μM). Additionally, the modified screen-printed electrode demonstrated excellent selectivity, enabling accurate insulin determination in the presence of interferences and in real blood serum samples. These findings highlight the potential of nickel-modified chitosan nanoparticles as a surface modification strategy to enhance the performance of electrochemical sensors insulin detection and pave the way for their application in various bioanalytes determination platforms.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100624"},"PeriodicalIF":10.61,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143912315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SPARK - Sensor platform for affinity recognition of paraquat 用于百草枯亲和力识别的SPARK传感器平台

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-05-02 DOI: 10.1016/j.biosx.2025.100625

Durgasha C. Poudyal , Vikram Narayanan Dhamu , Manish Samson , Sumana Karmakar , Sriram Muthukumar , Shalini Prasad

{"title":"SPARK - Sensor platform for affinity recognition of paraquat","authors":"Durgasha C. Poudyal , Vikram Narayanan Dhamu , Manish Samson , Sumana Karmakar , Sriram Muthukumar , Shalini Prasad","doi":"10.1016/j.biosx.2025.100625","DOIUrl":"10.1016/j.biosx.2025.100625","url":null,"abstract":"<div><div>The extensive use of paraquat (PQT) in agriculture has led to considerable environmental pollution and has raised serious health issues. Rapid, sensitive, and on-site detection tools are crucial for monitoring paraquat residue and preventing its toxic effect. This study focused on demonstrating a portable, label-free electrochemical sensor platform (SPARK) for the direct detection of paraquat using edamame soybean as food matrix. The sensor platform was fabricated using the excellent substrate property of a reduced graphene oxide (rGO), which was immobilized with the anti-paraquat antibody (PQT-Ab, specific to recognize the herbicide paraquat) using a linker molecule 1-pyrenebutanoic acid succinimidyl ester (Pyr-BASE). The rate of crosslinker adsorption on rGO was evaluated and demonstrated for the first time using electrochemistry, with the calculated monolayer adsorption of the pyrene-based crosslinker found to be 244 μC/cm<sup>2</sup>. The sensor performance was tested using portable device platform (SPARK) to confirm its feasibility, which showed a limit of detection (LOD) of 0.3 ng/mL (0.3 ppb), dynamic range of PQT from 0.3 to 72.9 ng/mL and an r<sup>2</sup> value of 0.98. The cross-reactivity study demonstrates high selectivity for the target PQT antigen, in presence of non-specific antigen such as glyphosate and chlorpyrifos. Pearson's correlation of r = 0.99 indicated a strong positive correlation between SPARK platform and the third-party gold standard method. Developed SPARK platform provides sensitive data comparable to traditional analytical methods in a simplified manner, thereby opening the possibility for electrochemical sensor platform to be used as on-site testing devices for various other food matrices.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100625"},"PeriodicalIF":10.61,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143902498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanotechnology-based paper microfluidics for rapid point-of-care detection and differentiation of snake venom types 基于纳米技术的纸微流体快速检测和区分蛇毒类型

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-04-21 DOI: 10.1016/j.biosx.2025.100623

Lakshmi Narashimhan Ramana , Nitin Salvi , M.V. Khadilkar , Tarun Kumar Sharma

{"title":"Nanotechnology-based paper microfluidics for rapid point-of-care detection and differentiation of snake venom types","authors":"Lakshmi Narashimhan Ramana , Nitin Salvi , M.V. Khadilkar , Tarun Kumar Sharma","doi":"10.1016/j.biosx.2025.100623","DOIUrl":"10.1016/j.biosx.2025.100623","url":null,"abstract":"<div><div>Snake envenomation is recognized as a neglected tropical disease, contributing to high mortality rates and causing significant organ damage, particularly to the liver, kidneys, and brain. The primary treatment involves administering antivenom, which consists of polyclonal antibodies developed against various snake venoms. However, antivenom therapy can lead to serum-related complications, reducing its effectiveness. Therefore, targeting specific therapeutic molecules could significantly improve snake envenomation treatment. Identifying the snake species is a major challenge due to their similar morphological characteristics. Globally, only two snake venom diagnostic kits are available that have been developed to detect country-specific snake venom. Hence, there is an urgent need to develop new diagnostic assays tailored for detecting venom specific to India. To address this, the current study focuses on detecting functional enzyme components of venomous snake species, such as phospholipase A2, hyaluronidase, and proteases. The study is based on the loading of the dye-loaded stimuli-responsive nanoparticles, including liposomes (sensitive to phospholipase A2), hyaluronic acid-chitosan nanoparticles (sensitive to hyaluronidase), and casein nanoparticles (sensitive to proteases) into paper-based microfluidics and tested with various snake venoms. The device successfully detects and distinguishes between wet bites and dry bites, as well as viper and elapid species.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"25 ","pages":"Article 100623"},"PeriodicalIF":10.61,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143916770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker 3D打印微孔板混合微流控装置中的纸盘用于低成本、快速、超灵敏的纸基生物发光检测人表皮生长因子受体2 （HER2）乳腺癌生物标志物

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-04-16 DOI: 10.1016/j.biosx.2025.100621

Ahmed A. Shalaby , Asmaa Salah , Akihiko Ishida , Masatoshi Maeki , Manabu Tokeshi

{"title":"Paper discs in a 3D printed microplate hybrid microfluidic device for low-cost, rapid, and ultrasensitive paper-based bioluminescence detection of human epidermal growth factor receptor 2 (HER2) breast cancer biomarker","authors":"Ahmed A. Shalaby , Asmaa Salah , Akihiko Ishida , Masatoshi Maeki , Manabu Tokeshi","doi":"10.1016/j.biosx.2025.100621","DOIUrl":"10.1016/j.biosx.2025.100621","url":null,"abstract":"<div><div>Breast cancer is the most common cancer type in women and it has the highest probability of developing into invasive cancer. Early detection of breast cancer is crucial to reduce the disease burden and decrease the mortality rate. Detection of cancer biomarkers is an attractive non-invasive way to implement early diagnosis and follow-up. Colorimetric enzyme-linked immunosorbent assay (ELISA) is one of the most common techniques used for the detection of cancer biomarkers. However, it requires a long incubation time and a large reagent volume, and it has low sensitivity. Here, we propose use of a paper disc in a 3D printed microplate hybrid microfluidic device for ultrasensitive paper-based bioluminescence ELISA for detection of HER2 breast cancer biomarker. Chromatographic paper discs are good substrates for fast immobilization of capture antibody without making any surface modification and they can be replaced with new discs to reuse the 3D printed microplate. The 3D printed microplate has microvalves in the bottom of the wells, so it can stop flow of the reagents for the desired incubation time and it allows the washing solution to flow vertically and drain onto an adsorption pad which increases the washing efficiency. NanoLuc luciferase was used as a label for the detection antibody to achieve the highest sensitivity. Bioluminescence sandwich ELISA for HER2 detection was performed using the hybrid device in just 20 min and the limit of detection was 1.3 fg/mL which is more than 10<sup>4</sup>-fold better than commercial ELISA kits for HER2.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100621"},"PeriodicalIF":10.61,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143854855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imatinib detection by memristive biosensors for therapeutic drug monitoring 记忆体生物传感器检测伊马替尼用于治疗药物监测

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-04-11 DOI: 10.1016/j.biosx.2025.100620

Junrui Chen , Lavinia Alberi , Yuan Pétermann , Thierry Buclin , Monia Guidi , Sandro Carrara

{"title":"Imatinib detection by memristive biosensors for therapeutic drug monitoring","authors":"Junrui Chen , Lavinia Alberi , Yuan Pétermann , Thierry Buclin , Monia Guidi , Sandro Carrara","doi":"10.1016/j.biosx.2025.100620","DOIUrl":"10.1016/j.biosx.2025.100620","url":null,"abstract":"<div><div>Therapeutic drug monitoring is essential for optimizing the efficacy and safety of targeted anticancer agents like imatinib, a first-line treatment for various leukemias and gastrointestinal stromal tumors. This study introduces a novel memristive biosensor designed for the detection of imatinib. The biosensor employs a silicon nanowire (SiNW) -based memristive architecture integrated with a single-stranded DNA (ssDNA) aptamer as the bio-recognition element. The detection of imatinib concentration is successfully demonstrated in both buffer and human plasma. Kinetic analysis reveals that the analysis time for achieving binding equilibrium and measurement is within 10 min. Comprehensive linear response over Imatinib concentrations in human plasma ranging from 0.2 μM to 20 μM was achieved, with a detection limit of 0.13 μM. While the interfering proteins such as human serum albumin (HSA) and α1-acid glycoprotein (AGP) compete with the binding mechanism, resulting in a decreased measured signal at lower concentrations of imatinib, their excessive presence paradoxically amplifies the measured signal. This amplification, however, also introduces increased variability in plasma measurements. This innovative memristive biosensor represents a significant advancement towards point-of-care therapeutic drug monitoring. It offers a robust and scalable platform, paving the way for the integration of personalized medicine into routine clinical workflows for imatinib-based therapies.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"24 ","pages":"Article 100620"},"PeriodicalIF":10.61,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0