Biosensors and Bioelectronics: X最新文献_第6页

Wearable smart sensors integration with AI and machine learning for tracking human health 集成人工智能和机器学习的可穿戴智能传感器，用于跟踪人类健康状况

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-11-12 DOI: 10.1016/j.biosx.2025.100711

Shi Xianyong , Dinesh Kumar Mishra , Öznur Özge Özcan , Mesut Karahan , Tan Panpan , Xiao Zhongshan , Palanirajan Vijayaraj Kumar

{"title":"Wearable smart sensors integration with AI and machine learning for tracking human health","authors":"Shi Xianyong , Dinesh Kumar Mishra , Öznur Özge Özcan , Mesut Karahan , Tan Panpan , Xiao Zhongshan , Palanirajan Vijayaraj Kumar","doi":"10.1016/j.biosx.2025.100711","DOIUrl":"10.1016/j.biosx.2025.100711","url":null,"abstract":"<div><div>This study comprehensively investigates the integration of wearable smart sensors with artificial intelligence (AI) and machine learning (ML) for human health tracking, focusing on seven major sensor types: sweat, glucose, wound, mental state monitoring, inhalation, CRISPR‒Cas, and quantum sensors. It elaborates on their design principles, detection mechanisms, and biomedical applications, as well as their respective advantages and inherent challenges. This study highlights the pivotal role of AI/ML in optimizing sensor performance, including enhancing detection sensitivity, processing complex data, enabling real-time analysis, and enabling personalized healthcare. Specifically, AI/ML facilitates noise filtering, pattern recognition, multibiomarker identification, and predictive diagnostics across different sensor systems. Despite significant advancements, the field is still confronted with challenges, including sensor stability, data security risks, high production costs, and biocompatibility issues. The paper concludes by outlining future research and development directions, emphasizing material innovation, algorithm optimization, and multimodal sensing integration, and strengthening clinical translation to fully unlock the potential of wearable sensors in proactive and personalized healthcare, ultimately contributing to the improvement of public health outcomes.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100711"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145576183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eprobe: A multi-technique, multi-channel wearable potentiostat Eprobe：一种多技术、多通道的可穿戴电位器

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-09-17 DOI: 10.1016/j.biosx.2025.100686

Jinhang Ma , Sijie Yin , Xiaoyan Wang , Qi Zhang , Minmin Liang

{"title":"Eprobe: A multi-technique, multi-channel wearable potentiostat","authors":"Jinhang Ma , Sijie Yin , Xiaoyan Wang , Qi Zhang , Minmin Liang","doi":"10.1016/j.biosx.2025.100686","DOIUrl":"10.1016/j.biosx.2025.100686","url":null,"abstract":"<div><div>This manuscript presents Eprobe, a wearable potentiostat that supports multiple electrochemical techniques and simultaneous multi-channel operations. Built on an ARM-based microcontroller, Eprobe incorporates an advanced analog front-end (AFE) optimized for versatile electrochemical functions. It provides a dynamic voltage regulation range of ±16 V, ensuring accurate and reliable measurements across various conditions. With compact dimensions of 6 cm × 7 cm × 2 cm and a weight of 150 g, Eprobe supports 12 electrochemical techniques across six independent channels operating concurrently. It provides a bias voltage output range of ±10 V, a current measurement range of ±200 mA, and a measurement accuracy of 100 nA. Comparative experiments with the CHI760E potentiostat―a leading benchtop device measuring 34 cm × 25 cm × 11 cm and weighing 4.5 kg―along with real-world measurements and simultaneous multichannel operation, demonstrate that Eprobe achieves comparable performance. Moreover, Eprobe offers significant advantages over existing wearable potentiostats, including an extended voltage support range, enhanced current detection sensitivity, and multi-channel, multi-technique compatibility. Eprobe is fully open-source, offering researchers a powerful tool to advance electrochemical methodologies and facilitate the development of wearable, multi-target integrated monitoring systems.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100686"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145119089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced bacterial accumulation on dielectrophoretic (DEP) structures via oscillatory flow induced by electrowetting-on-dielectric (EWOD) 电介质上电润湿（EWOD）诱导的振荡流增强细菌在介电结构上的积累

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-09-24 DOI: 10.1016/j.biosx.2025.100688

Marten Musiol , Fenja Schröder , Guiyu Wu , Marte Thorns , Felix Hirschberg , Annalena Eckert , Hans-Hermann Johannes , Wolfgang Kowalsky

{"title":"Enhanced bacterial accumulation on dielectrophoretic (DEP) structures via oscillatory flow induced by electrowetting-on-dielectric (EWOD)","authors":"Marten Musiol , Fenja Schröder , Guiyu Wu , Marte Thorns , Felix Hirschberg , Annalena Eckert , Hans-Hermann Johannes , Wolfgang Kowalsky","doi":"10.1016/j.biosx.2025.100688","DOIUrl":"10.1016/j.biosx.2025.100688","url":null,"abstract":"<div><div>In this work, we propose one possibility to increase both the speed and the quantity of bacteria collected on a dielectrophoresis (DEP) structure for small sample volumes. To achieve this, an electrowetting-on-dielectric (EWOD) device was positioned opposite to the DEP structure. This setup allows oscillations of the droplet containing the bacteria, leading to a continuous flow within the droplet that brings new bacteria into the vicinity of the DEP electrodes, where the bacteria can be captured. Additionally, the EWOD electrodes can be employed to transport small liquid samples containing bacteria towards the DEP electrodes. To reduce the voltage required for the EWOD operation, a thin film of titanium dioxide was incorporated into the structure. The thickness of this layer, as well as the hydrophobic coating necessary for optimal EWOD performance, was optimized. Polymer microspheres were used for oscillation testing, and <em>Escherichia coli</em> (<em>E. coli</em>) served as the primary test organism. The behavior of these microparticles in a liquid environment was monitored microscopically.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100688"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanopot V1.0: A point of care electrochemical sensor prototype device for precise detection of tacrolimus in transplant patients nanoot V1.0：用于移植患者他克莫司精确检测的护理点电化学传感器原型装置

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-10-23 DOI: 10.1016/j.biosx.2025.100708

K. Niyas , K.R. Anitha , Muhammad Salmun , P. Abdul Rasheed

{"title":"Nanopot V1.0: A point of care electrochemical sensor prototype device for precise detection of tacrolimus in transplant patients","authors":"K. Niyas , K.R. Anitha , Muhammad Salmun , P. Abdul Rasheed","doi":"10.1016/j.biosx.2025.100708","DOIUrl":"10.1016/j.biosx.2025.100708","url":null,"abstract":"<div><div>A miniaturized electrochemical sensor device prototype (NanoPot V1.0) encompasses with a portable potentiostat was developed for precise detection of tacrolimus, which is a critical immunosuppressant drug used for prevention of organ rejection in transplant patients. The developed NanoPot V1.0 prototype device combines a LMP91000 analog front-end with a ESP8266 microcontroller on a compact circuit board, along with a cylindrical well comprising of three electrode system with carbon cloth as working electrode. Notably, the chronoamperometric results from NanoPot V1.0 prototype device were very similar to those from a regular conventional CH Instruments potentiostat, which proved that the developed prototype device is accurate and reliable. The electrochemical behaviour of tacrolimus using NanoPot V1.0 prototype device was evaluated across a concentration range of 5–50 nM, which comes under clinically relevant levels. The prototype device showed excellent linearity with a detection limit comparable to standard laboratory equipment with excellent linearity (R<sup>2</sup> = 0.99) in tacrolimus quantification. Hence, this NanoPot V1.0 prototype device is bridging the gap between sophisticated laboratory-grade instruments and portable point-of-care (PoC) devices. This work represents a significant step towards developing a portable, sensitive, low-cost, and reliable PoC device for tacrolimus monitoring. This combination of miniaturized hardware and optimized electrochemical methods offers promising potential for PoC applications in transplant medicine and other broader bioanalytical fields.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100708"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical and MEMS-based biosensors for quantifying ALDH1A1 expression and activity in non-small cell lung cancer cell lines 电化学和mems生物传感器定量ALDH1A1在非小细胞肺癌细胞系中的表达和活性

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-10-21 DOI: 10.1016/j.biosx.2025.100703

Ali I.M. Ibrahim , Worood H. Ismail , Alaa M. Hammad , Osama H. Abusara , Khaldoon Al-Qawasmeh , Abass Al-Momany , Abdel Qader Al Bawab , Raed Shadfan , Alwathiqbellah Ibrahim

{"title":"Electrochemical and MEMS-based biosensors for quantifying ALDH1A1 expression and activity in non-small cell lung cancer cell lines","authors":"Ali I.M. Ibrahim , Worood H. Ismail , Alaa M. Hammad , Osama H. Abusara , Khaldoon Al-Qawasmeh , Abass Al-Momany , Abdel Qader Al Bawab , Raed Shadfan , Alwathiqbellah Ibrahim","doi":"10.1016/j.biosx.2025.100703","DOIUrl":"10.1016/j.biosx.2025.100703","url":null,"abstract":"<div><div>Aldehyde dehydrogenase 1A1 (ALDH1A1) is a key biomarker in cancer cells, associated with cancer stem cell properties and drug resistance. Quantifying and analyzing ALDH1A1 activity in cancer cells can provide crucial insights into cancer progression and mechanisms of resistance to treatment. This study explores the application of biosensors in detecting and measuring ALDH1A1 levels in two non-small cell lung cancer cell lines (NSCLC) (A549 and NCI-H1299) and assessing enzyme activity. We used advanced biosensor technology to evaluate enzyme expression in cell lines tested and their functional activity using specific substrates and cofactors of ALDH1A1 compared to their exposure to a known inhibitor. Our results demonstrate that biosensor-based assays can accurately measure ALDH1A1 expression and functional activity, distinguishing between different cancer cell populations. In addition, a complementary theoretical MEMS-based cantilever biosensor model was developed that shows predictable resonance frequency shifts with binding to ALDH1A1, thus providing a predictive framework for quantitative diagnostics and sensor optimization. These biosensors offer high sensitivity, specificity, and real-time monitoring capabilities, providing a valuable tool to understand the role of ALDH1A1 in cancer biology, developing targeted therapies, and improving clinical outcomes.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100703"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145412918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas12-based signal-on lateral flow immunoassay for ultra-sensitive, selective, and rapid detection of human papillomavirus DNA in clinical samples 基于CRISPR/ cas12的信号侧流免疫分析法用于临床样品中人乳头瘤病毒DNA的超灵敏、选择性和快速检测

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.biosx.2025.100689

Panon Tungkunaruk , Sakda Jampasa , Anchaleeporn Waritswat Lothongkum , Orawon Chailapakul , Natthaya Chuaypen , Janjira Panchompoo

{"title":"CRISPR/Cas12-based signal-on lateral flow immunoassay for ultra-sensitive, selective, and rapid detection of human papillomavirus DNA in clinical samples","authors":"Panon Tungkunaruk , Sakda Jampasa , Anchaleeporn Waritswat Lothongkum , Orawon Chailapakul , Natthaya Chuaypen , Janjira Panchompoo","doi":"10.1016/j.biosx.2025.100689","DOIUrl":"10.1016/j.biosx.2025.100689","url":null,"abstract":"<div><div>The trans-cleavage capability of CRISPR/Cas12a presents significant opportunities for the precise detection of double-stranded DNA (dsDNA). However, this potential has not been fully utilized in the context of lateral flow devices. This study introduced a novel, highly sensitive lateral flow assay that leverages the functionalities of CRISPR/Cas12a in conjunction with DNA probes to detect human papillomavirus (HPV) DNA, a primary etiological factor in cervical cancer, directly from clinical samples. The assay employed a DNA capture probe with specific sequences to construct both the control line using 40 thymine bases (T40) single-stranded DNA (ssDNA) and the test line utilizing 40 cytocine bases (C40) ssDNA, thereby effectively monitoring the presence of target DNA. In the mixed master reaction, CRISPR/Cas12a was combined with Biotin-GGGGGGGGGGAAAAAAAAAA (G10A10) ssDNA and introduced to a sample containing the HPV DNA. This process initiated a trans-cleavage mechanism, resulting in the conversion of Biotin-G10A10 ssDNA into Biotin-G10 ssDNA. By incorporating streptavidin-conjugated gold nanoparticles on the conjugate pad, the assay captured both Biotin-G10 ssDNA and any unreacted Biotin-G10A10 ssDNA at the test line and control line, respectively, leading to a distinct colorimetric appearance. Under optimized conditions, the assay demonstrated a detection limit of 0.2 copies/μL and exhibited excellent selectivity for HPV16 in clinical samples, correlating favorably with results obtained through gel electrophoresis. This innovative system not only underscores the considerable potential for rapid, sensitive, and specific detection of HPV DNA, but it also serves as a promising prototype for further advancements in nucleic acid-based detection methodologies.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100689"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145217257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach for non-invasive, simultaneous, and realtime monitoring of BCG, BPW, and heart sounds with flexible and bio-compatible piezoelectric AlN sensor 一种无创、同步、实时监测BCG、BPW和心音的新方法，采用柔性和生物兼容的压电AlN传感器

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-09-25 DOI: 10.1016/j.biosx.2025.100693

Marco Cinquino , Suleyman Mahircan Demir , Angela Tafadzwa Shumba , Luca Fachechi , Francesco Rizzi , Antonio Qualtieri , Luigi Patrono , Vincenzo Mariano Mastronardi , Massimo De Vittorio

{"title":"A novel approach for non-invasive, simultaneous, and realtime monitoring of BCG, BPW, and heart sounds with flexible and bio-compatible piezoelectric AlN sensor","authors":"Marco Cinquino , Suleyman Mahircan Demir , Angela Tafadzwa Shumba , Luca Fachechi , Francesco Rizzi , Antonio Qualtieri , Luigi Patrono , Vincenzo Mariano Mastronardi , Massimo De Vittorio","doi":"10.1016/j.biosx.2025.100693","DOIUrl":"10.1016/j.biosx.2025.100693","url":null,"abstract":"<div><div>Monitoring physiological cardiovascular parameters such as heart rate, heart rate variability (HRV), heart sounds, blood pressure wave (BPW), pulse wave velocity (PWV), and cardio-ankle vascular index (CAVI) is crucial for the early diagnosis of cardiovascular diseases. While hospital monitoring devices are consistently reliable, they are often expensive, bulky, and require well-trained personnel. Low-cost, highly accessible, and easy-to-use devices that continuously monitor these parameters are extremely important as they can address these issues, helping individuals to stay informed about their health status. Here is reported a new method for simultaneously measuring cardiovascular bio-indicators by analyzing Ballistocardiogram (BCG), BPW, and heart sound traces, achieved by a single acquisition, exploiting a thin, flexible, bio-compatible, and non-invasive piezoelectric sensor based on a 1 μm thick aluminum nitride film and a flexible 25 μm thick Kapton substrate. A CAVI = 6.8 ± 0.3, and the other 26 parameters are measured from four arterial pulse sites – carotid, brachial, radial, and posterior tibial arteries – providing a comprehensive subject's health profile. The proposed approach offers a non-invasive method for continuous health monitoring, exploiting a bio-compatible, low-cost, and low-power technology that can also be a powerful tool for disease prevention, facilitating personalized medical diagnosis and therapies simultaneously.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100693"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Field-effect transistor for biosensing applications using a graphene channel with amine-rich coatings 用于生物传感应用的场效应晶体管，使用石墨烯通道和富胺涂层

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-09-02 DOI: 10.1016/j.biosx.2025.100673

Trung T. Pham , Jean-François Colomer , José Ignacio Veytia-Bucheli , Benjamin Ledoux , Henri-François Renard , Cédric R. Vandenabeele , Laurent Houssiau , Laurent A. Francis , Stéphane P. Vincent , Robert Sporken

{"title":"Field-effect transistor for biosensing applications using a graphene channel with amine-rich coatings","authors":"Trung T. Pham , Jean-François Colomer , José Ignacio Veytia-Bucheli , Benjamin Ledoux , Henri-François Renard , Cédric R. Vandenabeele , Laurent Houssiau , Laurent A. Francis , Stéphane P. Vincent , Robert Sporken","doi":"10.1016/j.biosx.2025.100673","DOIUrl":"10.1016/j.biosx.2025.100673","url":null,"abstract":"<div><div>Since graphene has a unique band structure with the valence and conduction bands touching each other at a single point called the Dirac point, this makes it extremely sensitive to the surroundings such as doping, external electric field, mechanical deformation, etc. Hence, it is very desirable for sensing applications. However, its surface inertness poses significant drawbacks. Therefore, it is necessary to treat the graphene surface to bind biomolecules. In this paper, we report the use of amine-functionalized graphene by plasma polymerization to detect the presence of biomolecules in graphene channel based on a liquid-gate field-effect transistor (LG-GFET). Taking streptavidin and biotin as an example, the binding interactions of streptavidin–biotin complexes are detected by monitoring the shift of the Dirac point. By varying the streptavidin concentrations from 0.1 nM to 1000 nM, we found that our LG-GFET achieves detection capabilities as low as 0.1 nM. Our approach can be applied for the detection of biological molecules with low detection limit, high sensitivity, and stability.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100673"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing nanowell biosensor fabrication: Evaluation of metal deposition and oxide layer modification 优化纳米孔生物传感器制造：金属沉积和氧化层修饰的评价

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-11-22 DOI: 10.1016/j.biosx.2025.100713

Ali Fardoost, Mehdi Javanmard

{"title":"Optimizing nanowell biosensor fabrication: Evaluation of metal deposition and oxide layer modification","authors":"Ali Fardoost, Mehdi Javanmard","doi":"10.1016/j.biosx.2025.100713","DOIUrl":"10.1016/j.biosx.2025.100713","url":null,"abstract":"<div><div>This study presents a comparative evaluation of two nanowell-based biosensor fabrication methods designed to improve sensing performance and device reliability. The conventional process employs electron beam (e-beam) evaporation for metal deposition and aluminum oxide (Al<sub>2</sub>O<sub>3</sub>) as the insulating layer, while the modified approach utilizes the sputtering method for metal deposition and hafnium oxide (HfO<sub>2</sub>) as the insulating layer, respectively. Both fabrication methods were used to construct nanowell impedance biosensors for detecting interleukin-6 (IL-6) at concentrations ranging from 10 nM to 10 fM. Electrochemical impedance spectroscopy (EIS) was performed in a two-electrode configuration using an AC excitation of 10 mV across a frequency range of 1 Hz–5 MHz. The Sputtered/HfO<sub>2</sub> biosensors exhibited improved electrical insulation, enhanced step coverage, and reduced device failure due to short or open circuits. These improvements resulted in a higher baseline response, reduced voltage drift, and enhanced sensitivity, achieving a limit of detection (LOD) of 7.8 fM compared to 15.5 fM for the E-beam/Al<sub>2</sub>O<sub>3</sub> sensors. The results demonstrate that optimized fabrication using sputtering and HfO<sub>2</sub> significantly enhances biosensor reproducibility and detection performance, supporting its application in scalable, label-free diagnostic platforms.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100713"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145620175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A screen-printed carbon electrode immobilized MXene-gold nanoparticles composite detects creatinine in micro-volume of human serum samples 丝网印刷碳电极固定化mxene -金纳米颗粒复合材料检测微量人血清样品中的肌酐

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2025-12-01 Epub Date: 2025-10-31 DOI: 10.1016/j.biosx.2025.100710

Monika Chhillar , Poornima Bhatt , Anup Singh , Deepak Kukkar , Ashok Kumar , Ashok Kumar Yadav

{"title":"A screen-printed carbon electrode immobilized MXene-gold nanoparticles composite detects creatinine in micro-volume of human serum samples","authors":"Monika Chhillar , Poornima Bhatt , Anup Singh , Deepak Kukkar , Ashok Kumar , Ashok Kumar Yadav","doi":"10.1016/j.biosx.2025.100710","DOIUrl":"10.1016/j.biosx.2025.100710","url":null,"abstract":"<div><div>Creatinine (CR), a non-protein nitrogenous compound, serves as an important clinical indicator for assessing renal function. This research reports the application of screen-printed electrodes modified with Ti<sub>3</sub>C<sub>2</sub>-based MXene-gold nanocomposites (SPCE/MXene/AuNPs) for the precise quantification of CR in human serum. The SPCE/MXene/AuNPs sensor leverages its interactions with CR to enhance redox electrochemical signals, leading to their amplified detection sensitivity. Under optimal conditions (pH 7.4), the SPCE/MXene/AuNPs sensor exhibited excellent analytical performance, with a linear detection range of 0.1–11 mg dL<sup>−1</sup>, a high correlation coefficient (R<sup>2</sup> = 0.99), and calculated detection and quantification limits of 0.018 mg dL<sup>−1</sup> and 0.06 mg dL<sup>−1</sup>, respectively. Additionally, these electrodes demonstrated minimal interference from common physiological compounds (glucose, ascorbic acid, glutathione, bilirubin, gentamicin, vancomycin, arginine globulin, histidine, urea, bovine serum albumin (BSA), and electrolytes [K<sup>+</sup>, Ca<sup>2+</sup>, SO<sub>4</sub><sup>2−</sup>, and Mg<sup>2+</sup>]). Furthermore, the developed sensor demonstrated excellent correlation (R<sup>2</sup> = 0.99) with the standard Jaffe method for CR assessment in human serum samples (n = 89) with a mean difference of −0.136 mg dL<sup>−1</sup> (95 % CI: 0.190 to −0.081 mg dL<sup>−1</sup>) and significant agreement confirmed by paired <em>t</em>-test (p < 0.0001). Only four samples exhibited false negatives (3.58, 3.02, 9.50, and 11.25 %). The sensor retained 94.7 % of its initial response after 15 days at 4 °C, and reproducibility was confirmed via overlapping CV cycles. Our investigation demonstrates the potential of our electrochemical sensor for accurate and reliable CR measurement in complex biological matrices.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"27 ","pages":"Article 100710"},"PeriodicalIF":10.61,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145462872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0