Biosensors and Bioelectronics: X最新文献

{"title":"In-SITE: In situ soil topological examination platform for hydration state, volumetric density and carbon stocks assessment","authors":"Vikram Narayanan Dhamu ,&nbsp;Diya Baby ,&nbsp;Mohammed Eldeeb ,&nbsp;Sriram Muthukumar ,&nbsp;Shalini Prasad","doi":"10.1016/j.biosx.2024.100559","DOIUrl":"10.1016/j.biosx.2024.100559","url":null,"abstract":"<div><div>This study presents a novel application of electrochemical impedance spectroscopy (EIS) for comprehensive soil health assessment, with a particular emphasis on bulk density (BD) analysis. Conventional methods for BD measurement, while effective, can be labor-intensive and prone to inaccuracies, especially in varying field conditions. In contrast, EIS offers a reliable, cost-effective, and non-invasive solution for real-time, in-situ measurements. The research outlines the use of Room Temperature Ionic Liquid (RTIL) functionalized sensors and the development of a portable hardware system for field deployment. Experimental protocols involved collecting impedance data by sweeping from 50 kHz to 5 Hz across various soil types to construct soil moisture profiles, and calibration curves demonstrated a strong correlation between impedance values and soil moisture content, validating the method's accuracy. The study introduces the DENSE model, which uses impedance data to predict soil volumetric density, with results closely matching standard mass/volume techniques. Further analysis reveals the complex relationships between soil moisture, BD, and impedance, producing calibrated models for accurate BD prediction. Additionally, the study extends to carbon stock analysis by integrating data on soil organic carbon percentage, BD, and soil depth to estimate carbon levels. Collectively, these findings underscore the potential of this EIS-based sensor as a versatile and precise tool for real-time soil health monitoring, offering significant contributions to precision agriculture and sustainable farming practices.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100559"},"PeriodicalIF":10.61,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox-active molecules in bacterial cultivation media produce photocurrent","authors":"Matthew C. Smith ,&nbsp;Nathan S. Nasseri ,&nbsp;Emile J. Morin ,&nbsp;Jakkarin Limwongyut ,&nbsp;Alex S. Moreland ,&nbsp;Yaniv Shlosberg ,&nbsp;Andrea S. Carlini","doi":"10.1016/j.biosx.2024.100558","DOIUrl":"10.1016/j.biosx.2024.100558","url":null,"abstract":"<div><div>Renewable energy concepts such as microbial fuel cells (MFCs) present a promising, yet intrinsically complex electrochemical approach for utilizing bacteria as an electron source. In this work, we show that just the cultivation media for bacterial growth, which is based on yeast extract, is sufficient for generating electrical current in a bio-electrochemical cell (BEC). We apply cyclic voltammetry and 2-dimensional fluorescence spectroscopy to identify redox active molecules such as NADH, NAD⁺, and flavines that may play key roles in electron donation. Finally, we show that upon illumination, current production is enhanced 2-fold. This photocurrent is generated by a variety of metabolites capable of photochemical reduction, enabling them to donate electrons at the anode of the BEC.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100558"},"PeriodicalIF":10.61,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Printed dry and ready-to-use in vitro diagnostic culture media devices for differentiation and antimicrobial susceptibility testing of bacteria","authors":"Dieter Spiehl ,&nbsp;Gerhard Schwall ,&nbsp;Fabian Post ,&nbsp;Carina Weber ,&nbsp;Edgar Dörsam ,&nbsp;Andreas Blaeser ,&nbsp;Volkhard A.J. Kempf ,&nbsp;Michael Hogardt","doi":"10.1016/j.biosx.2024.100557","DOIUrl":"10.1016/j.biosx.2024.100557","url":null,"abstract":"<div><div>This work presents a ready-to use dry culture device platform for differentiation of bacterial species and testing antibiotic susceptibility conveniently. The study demonstrates that high-volume and scalable production methods such as can reliably manufacture such devices for reproducible in vitro diagnostic testing. This marks a significant advancement compared to similar handmade devices in the literature.</div><div>Two types of ready-to-use (RTU) devices were produced through printing. One device differentiates bacterial species from urine samples for rapid diagnosis of urinary tract infections (UTI), while the other determines the minimum inhibitory concentration (MIC) of antibiotics against specific bacterial species. The results show performance comparable to standard tests in clinical microbiological laboratories. The differentiation of bacterial species from four UTI samples matched the efficacy of commercial UTI agar plates utilizing chromogenic substrates. The MIC determination for seven bacterial species in three independent tests yielded categorical results consistent with MIC test strips on agar plates as reference standard. Minor errors occurred in 14 % and major errors in 5 % of the tests. No major errors occurred and in 81 % of all tests results were correct and consistent with the reference method.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100557"},"PeriodicalIF":10.61,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142587183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of novel DNA aptamers and colorimetric nanozyme aptasensor for targeting multi-drug-resistant, invasive Salmonella typhimurium strain SMC25","authors":"Akanksha Joshi ,&nbsp;Abhishek Kaushik ,&nbsp;Neetu Kumra Taneja ,&nbsp;Komal Chauhan ,&nbsp;Amit Kumar ,&nbsp;Tarun Kumar Sharma","doi":"10.1016/j.biosx.2024.100555","DOIUrl":"10.1016/j.biosx.2024.100555","url":null,"abstract":"<div><div>Invasive, biofilm-forming Non-typhoidal <em>Salmonella</em> (iNTS), propagating through the global food and water supply chain, presents a significant risk to food safety and public health. Developing a robust detection system is crucial for enabling point-of-care, affordable, and equipment-free identification of this pathogen throughout the supply chain. In this study, we screened a novel pool of ssDNA aptamers specific to a multidrug resistant iNTS strain SMC25, previously isolated from Indian poultry products in our earlier research. Through 13 rounds of whole-cell SELEX, we identified, characterized, and selected seven full-length aptamers (ST18, ST19, ST25, ST28, ST29, ST31, and ST32). Flow cytometric analysis reveals superior binding of ST25, ST28, ST29, and ST31. These aptamers were translated onto Nanozyme-based aptasensing system for efficient, cost-effective detection of SMC25. This system harnesses the aptamer-mediated, reversible peroxidase-like activity of gold nanoparticles (GNPs) to oxidize the TMB substrate into a one-electron oxidation state, resulting in a blue-colored Diamine charge transfer complex (DCTC). The catalytic process, coupled with GNP aggregation, induces a visible color change in the test mixture from ruby-red to blue. Post-SELEX truncations identified the optimal aptamer sequence (T_ST31), which selectively detected SMC25 in water with a limit of detection (LOD) of ∼10⁴ CFU/mL. Lower concentrations (10 CFU/mL) of SMC25 could be detected after non-selective enrichment within 120 min. This research introduces a novel pool of iNTS-specific aptamers along with a cost-effective (0.25 USD per sample) solution for colorimetric detection by the naked eye.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100555"},"PeriodicalIF":10.61,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance of label-free biosensors as a function of layer thickness","authors":"Tommi Åman ,&nbsp;Sanna Auer ,&nbsp;Vesa P. Hytönen ,&nbsp;Juha A. Määttä","doi":"10.1016/j.biosx.2024.100556","DOIUrl":"10.1016/j.biosx.2024.100556","url":null,"abstract":"<div><div>Biosensors are used in various applications in the field of medicine, environmental monitoring, and chemical processing to study the concentration and quality of target molecules. Biosensors convert the interaction between a specific target molecule and a recognition element into a measurable signal. We compared the performance of four different label-free biosensor techniques (multi-parametric surface plasmon resonance (MP-SPR), quartz crystal microbalance (QCM), mass-sensitive micro array (MSMA, or also known as film bulk acoustic resonators (FBARs)) and biolayer interferometry (BLI)) to evaluate how well they can quantify thick protein layers. We utilized the avidin-biotin system, which enables tight and specific binding and allowed us to assemble layers of proteins on the biosensor surface in a well-defined and reproducible fashion. Our results show that MP-SPR outperforms the other label-free biosensors in analyzing thick samples, showing a predictable and sensitive binding signal for over 50 albumin – avidin layers, which is estimated to correspond to a 300–400 nm thick protein layer. The linear measurement range of BLI was 38 layers corresponding to a 228–304 nm thick surface on the biosensor while QCM and MSMA were able to measure 108–144 nm and 72–96 nm thick protein layers with a fairly linear response, respectively.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100556"},"PeriodicalIF":10.61,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simple and sensitive method for in vitro monitoring of red blood cell viscoelasticity by Quartz Crystal Microbalance with dissipation monitoring (QCM-D)","authors":"Simonetta Palleschi ,&nbsp;Leopoldo Silvestroni ,&nbsp;Barbara Rossi ,&nbsp;Simone Dinarelli ,&nbsp;Marco Magi ,&nbsp;Lorenzo Giacomelli ,&nbsp;Andrea Bettucci","doi":"10.1016/j.biosx.2024.100554","DOIUrl":"10.1016/j.biosx.2024.100554","url":null,"abstract":"<div><div>Viscoelasticity (VE) is the intrinsic mechano-dynamic property enabling red blood cells (RBCs) to undergo prompt and repeated deformations while maintaining structural integrity. Assessing RBC VE and how different stressors can affect it is of great interest. Quartz Crystal Microbalance with Dissipation monitoring (QCM-D) is a technology exploiting high-frequency acoustic waves to probe soft matter rheological properties. In the present study, a QCM-D method is reported for <em>in vitro</em> monitoring of cell VE in viable RBCs. The method is based on casting a sensor-adherent cell monolayer and modeling it as an effective viscoelastic medium, and allows to extrapolate proxy values of both the elastic and the viscous cell shear moduli. Real-time VE changes induced by the known cell VE stressors temperature, medium tonicity, glutaraldehyde, methyl-β-cyclodextrin and cytochalasin D have been reliably identified. The method is relatively simple and inexpensive, non-invasive, and able to seize subtle changes of cell biomechanics. Hence, it could be usefully exploited for <em>in vitro</em> assessment of RBC rheological properties and their alterations induced by external chemico-physical stimuli.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100554"},"PeriodicalIF":10.61,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142533621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted biosensors for intracellular lipid droplet content detection","authors":"Shengdong Li","doi":"10.1016/j.biosx.2024.100551","DOIUrl":"10.1016/j.biosx.2024.100551","url":null,"abstract":"<div><div>Lipid droplets participate in the metabolic process of many living cells, and their abnormalities are highly correlated with many diseases such as fatty liver, diabetes, and cancer. Nevertheless, the current methods are inadequate for elucidating the relationship between diverse diseases and lipid droplets. In particular, the visualization of tumors with abnormal distribution of lipid droplets remains a significant challenge. Therefore, a water-soluble biosensor with lipid droplet specificity is synthesized by copolymerization of coumarin derivatives with low toxicity and high hydrophilic hydroxyl terminal polyethylene glycol. The survival rate of HeLa cells cultured with lipid drop biosensor for 24 h was more than 90% on average, and the toxicity was less. The co-localization imaging experiments with lipid droplets showed that the biosensor had a good ability to target lipid droplets in HeLa cells. The experimental results of the response of different cell lines to the biosensor showed that the tumor cells had a good response and uptake ability. These results indicate that the prepared biosensor has a good distinguishing function between normal cells and tumor cells, and provides an experimental basis for the early diagnosis of clinical tumors.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100551"},"PeriodicalIF":10.61,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intact photosynthetic bacteria-based electrodes for self-powered metal ions monitoring","authors":"Jefferson Honorio Franco ,&nbsp;Paolo Stufano ,&nbsp;Rossella Labarile ,&nbsp;Dario Lacalamita ,&nbsp;Pierluigi Lasala ,&nbsp;Elisabetta Fanizza ,&nbsp;Massimo Trotta ,&nbsp;Gianluca Maria Farinola ,&nbsp;Matteo Grattieri","doi":"10.1016/j.biosx.2024.100552","DOIUrl":"10.1016/j.biosx.2024.100552","url":null,"abstract":"<div><div>The low-cost and early monitoring of metal ion contaminants is paramount to prevent widespread contamination of water environments. Self-powered microbial electrochemical sensors represent an interesting approach to achieving this goal. Purple non-sulfur bacteria have a versatile metabolism and a well-characterized photosynthetic system, making them an ideal candidate for developing biohybrid technologies. In this work, we report the use of these bacteria in biophotoelectrodes to develop self-powered monitoring systems for two common pollutants, NiCl₂ and CuSO₄. The microbial biophotoelectrode was obtained on a homemade poly-hydroxybutyrate-carbon nanofibers electrode modified with a redox-adhesive polydopamine matrix-based entrapping the purple bacterium <em>Rhodobacter capsulatus</em>. The presence of 500 μM NiCl₂ resulted in a 60 % decrease in current density, while the simultaneous presence of 100 μM NiCl₂ and 100 mM CuSO₄ led to an 83 % current inhibition. Given the implementation of the biophotoelectrode in the field, the biohybrid system was tested in a complex matrix containing beer, demonstrating the promising ability of the photoelectrochemical system to act as an efficient biosensor in complex solutions. Finally, the biohybrid electrode was coupled to a cathode performing oxygen reduction, which allowed obtaining a self-powered monitoring system, paving the way for the future implementation of a low-cost monitoring system for widespread metal ions contaminant monitoring.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100552"},"PeriodicalIF":10.61,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142433226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel approach to tyrosinase-based biosensors: Electrode reactions and biological measurement","authors":"Masumeh Goodarzi ,&nbsp;Hosna Tavakoli ,&nbsp;Maryam Arab Khalaj ,&nbsp;Hassan Tavakoli","doi":"10.1016/j.biosx.2024.100550","DOIUrl":"10.1016/j.biosx.2024.100550","url":null,"abstract":"<div><div>This study introduces a novel tyrosinase-based biosensor designed to simultaneously detect tyrosinase, tyrosine, L-DOPA, and L-DOPA quinone. The biosensor, designated as GCE/GNP/Cys/Chit/Tyrase, was developed by immobilizing tyrosinase on a modified glassy carbon electrode (GCE) that incorporates electrodeposited gold nanoparticles, cysteine, and chitosan. The morphology of the biosensor was characterized using scanning electron microscopy (SEM). The electrochemical behaviors of the biosensor were explored in response to the target analytes. Key analytical characteristics were assessed, including linear range, sensitivity, selectivity, limits of detection and quantification, long-term stability, repeatability, reproducibility, electrochemically active surface area, and charge transfer behavior. The biosensor demonstrated a linear response range of 1–120 μM, with an impressive sensitivity of 200.4 mA.Lmol⁻¹cm⁻² and a detection limit of 27 μM. This study provides a comprehensive evaluation of the analytical features of tyrosinase-based biosensors. Additionally, the biosensor was applied to quantify dopamine in brain tissue, utilizing a calibration curve derived from the fabricated biosensor. The dopamine concentration measured in five Wistar rats was 35 ± 2.75 μM, reflecting the mean and standard deviation, respectively. These results confirm the biosensor's capability for accurately detecting this critical neurotransmitter in the brains of Wistar rats. This investigation underscores the potential of tyrosinase-based biosensors for diverse analytical applications in biological samples.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100550"},"PeriodicalIF":10.61,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An electroactive microfluidic platform integrated with AM-pDEP focusing and side-counter design for selective cell sorting and single-cell quantification","authors":"Zuyuan Tian ,&nbsp;Mohamed Shaheen ,&nbsp;Tianxiang Jiang ,&nbsp;Shaoxi Wang ,&nbsp;Xihua Wang ,&nbsp;Jie Chen","doi":"10.1016/j.biosx.2024.100549","DOIUrl":"10.1016/j.biosx.2024.100549","url":null,"abstract":"<div><div>Rapid isolation and precise quantification of target cancer cells are crucial for precision medicine applications. Conventional fluorescence-based methods require bulky optical instrumentation and specialized expertise. Recent advances in label-free microfluidic techniques have attempted to overcome these limitations but often suffer from reduced reliability and throughput when integrating multiple functions. Here, we demonstrate an electroactive microfluidic platform that integrates functions of selective concentration, particle focusing, and single-cell level quantification without introducing additional complex physical microstructures. Our device employs the dielectrophoresis (DEP) effect combined with an interdigitated cell sorter to achieve selective cell concentration. For quantification and characterization, the device leverages the impedimetric Coulter principle to achieve precise particle counting and characterization at single-cell resolution. To address the inherent tradeoff between sensitivity and throughput in the traditional Coulter counter, we utilize the tilted interdigitated electrode configuration excited by an amplitude-modulated (AM) Positive DEP (pDEP) signal instead of the commonly employed narrow channel or sheath flow to realize the effective focusing and alignment of the cell stream. The focusing module is combined with our side-counter design to detect cells. The performance of our device and its embedded modules has been verified with mixed breast cancer and blood model cell lines, where high consistency between optical and electrical detection has been demonstrated. We expect the integration of the proposed AM-pDEP focusing approach and the corresponding microfluidic design for cell sorter and on-chip flow cytometry would offer an alternative engineering solution to the stream-based on-chip single-cell analysis where reliable cell focusing and single-cell sensing are required.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100549"},"PeriodicalIF":10.61,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}