Biosensors and Bioelectronics: X最新文献

{"title":"Reduced amplification by phi29 DNA polymerase in the presence of unbound oligos during reaction in RCA","authors":"Darío Sánchez Martín ,&nbsp;Tingting Li ,&nbsp;Marie Wrande ,&nbsp;Linus Sandegren ,&nbsp;Bo Tian ,&nbsp;Maria Strømme ,&nbsp;Teresa Zardán Gómez de la Torre","doi":"10.1016/j.biosx.2024.100456","DOIUrl":"10.1016/j.biosx.2024.100456","url":null,"abstract":"<div><p>Synthetic single-stranded oligonucleotides play crucial roles in DNA amplification reactions for various applications, such as serving as primers, enabling magnetic separation, and generating dsDNA for subsequent digestion. Typically, these oligos are added in excess to ensure rapid binding to their intended targets. However, while performing rolling circle amplification (RCA) using phi29 DNA polymerase, we observed a decrease in amplification efficiency when oligos were present in the reaction. This phenomenon was consistently observed in two separate laboratories, prompting this study to delve into the root causes responsible for the decline in RCA efficiency. The lowered efficiency was consistent regardless of the manufacturer or any mutations in the phi29 polymerase. We identified several variables that influenced RCA efficiency, mainly the length of the oligos used and the presence of modifications, particularly those obstructing 3’ end digestion. This strongly suggests that the exonuclease domain of phi29 DNA polymerase is responsible for the competition-based inhibition. Our investigation shows that even picomole quantities of oligos can significantly reduce total DNA production during the phi29 DNA polymerase-mediated amplification process. Conversely, the addition of oligos to the reaction did not impede the efficiency of Bst 3.0 polymerase, likely due to the lack of an exonuclease domain of said polymerase. While increasing the quantity of phi29 DNA polymerase in the reaction partially alleviated the adverse effects of excess oligos, we believe it is crucial to carefully optimize the oligo quantities to achieve maximum amplification of the desired targets.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100456"},"PeriodicalIF":10.61,"publicationDate":"2024-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000207/pdfft?md5=d287c9aee31a57315a7717b990891ab8&pid=1-s2.0-S2590137024000207-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139872072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrio vulnificus marine pathogen detection with thin-film impedance biosensors","authors":"Arnau Pérez Roig ,&nbsp;Bergoi Ibarlucea ,&nbsp;Carmen Amaro ,&nbsp;Gianaurelio Cuniberti","doi":"10.1016/j.biosx.2024.100454","DOIUrl":"https://doi.org/10.1016/j.biosx.2024.100454","url":null,"abstract":"<div><p><em>Vibrio vulnificus</em> (Vv) is a marine pathogen that can cause rapid death by septicemia (vibriosis) in humans and several fish species. This pathogen is considered a biomarker of climate change, as both its presence and vibriosis incidence in coastal environments are increasing because of global warming. Currently, gold-standard methods for Vv detection are all PCR-based, requiring expensive equipment and skilled personnel, which hinders their use on a global scale. The aim of this work was to design and test a more affordable method that could be used worldwide for both vibriosis diagnosis and pathogen monitoring in water. To this end, we functionalized thin film microelectrodes with thiolated single-stranded DNA sequences complementary to the species-specific genetic marker, the gene <em>vvha</em>, and monitored the impedance changes upon hybridization. We tested the biosensor specificity with synthetic and natural DNA samples (from cultures of Vv and <em>V. cholerae</em>, a closely related species) and determined the detectable concentration range. The results obtained showed that this biosensor was specific for Vv, achieving detection down to 1 pM synthetic DNA and DNA extracted from 10² bacteria mL⁻¹, which is equivalent to that obtained by PCR. Consequently, this biosensor could be used on a global scale for vibriosis diagnostics, health risk studies and climate change monitoring, with potential application for <em>in situ</em> detection.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100454"},"PeriodicalIF":10.61,"publicationDate":"2024-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000189/pdfft?md5=af3b0aefae9767e5f3c6747487932fd8&pid=1-s2.0-S2590137024000189-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139737797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable optical biosensor for point-of-care monitoring of salivary glucose using a paper-based microfluidic strip","authors":"Shweta Panwar ,&nbsp;Paulami Sarkar ,&nbsp;D. Syed Kasim ,&nbsp;Raksha Anand ,&nbsp;Akanksha Priya ,&nbsp;Shyam Prakash ,&nbsp;Sandeep Kumar Jha","doi":"10.1016/j.biosx.2024.100452","DOIUrl":"10.1016/j.biosx.2024.100452","url":null,"abstract":"<div><p>The manuscript describes a technique for fabrication and validation of a standalone handheld optical biosensor designed for non-invasive monitoring of glucose through saliva. In this cost-effective process, a 3D-printed glucose test strip was filled with sieving paste comprising of cellulose, polyethylene glycol (PEG), polyvinyl alcohol (PVA) and glycerol, onto which, glucose oxidase-peroxidase (GOD-POD) enzymes and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) chromogenic dye were co-immobilized. The enzymatic reaction produced H₂O₂ as by-product with which the ABTS reacted, leading to colour change on the detection zone of the strip which was detected by the developed glucometer. The in-house developed meter included an optically isolated section in its structure for inserting the strip to prevent interference from the ambient light conditions. The biosensor exhibited a broad detection range of 28–204 mg/dL of glucose concentration, with a sensitivity of 26.89 count/mg/dL and a limit of detection (LOD) at 28 mg/dL, within a response time of 120 s. The device along with strips was validated with clinical samples, comparing salivary glucose levels (SGL) to blood glucose levels (BGL) using a commercial glucometer i.e., Accu-Chek Active. Student's t-test on clinical data yielded p-values of 0.018, 0.01, 0.008, and 0.003 in fasting and post-prandial samples of non-diabetic and diabetic patients respectively, which represents a significant correlation. The device also passed Clarke's error grid analysis and is hence considered medically acceptable. The low-cost and simple-to-use saliva-based glucometer should be ideally suited for mass screening of diabetes as well as day-to-day health check-ups in a non-invasive and painless manner.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100452"},"PeriodicalIF":10.61,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000165/pdfft?md5=6079c2eb7aa736c6042d9fe826728f82&pid=1-s2.0-S2590137024000165-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139817391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-enzymatic electrochemical detection of methylglyoxal in saliva using a polyaniline/nickel oxide nanohybrid biosensor: A noninvasive approach for diabetes diagnosis","authors":"Subramanian Vasanth,&nbsp;Humayun Amir,&nbsp;Nagomony Ponpandian,&nbsp;Chinnuswamy Viswanathan","doi":"10.1016/j.biosx.2024.100444","DOIUrl":"10.1016/j.biosx.2024.100444","url":null,"abstract":"<div><p>Methylglyoxal (MG) is a prominent biomarker for diabetic syndromes and ageing disorders. In the present study, a non-enzymatic electrochemical detection of MG in human saliva at low levels was achieved via polyaniline/nickel oxide nanohybrid modified graphite sheet (PANI/NiO/GS) electrode. The physical characteristics and structure of the PANI/NiO nanohybrid showed the presence of NiO flakes embedded within the irregular granular like structure of PANI matrices. Chronoamperometric (CA) analysis of the nanohybrid electrode showed excellent electrocatalytic activity towards MG with the linear range from 1 to 10 μM in 0.1 M phosphate buffer (pH 7). The proposed sensor boasts a high sensitivity of 1.136 μA.μM⁻¹ including a lower limit of detection of 2.64 nM. The real-time functionality of the proposed biosensor was also employed to estimate the precise quantification of MG levels in both healthy and diabetic patients' saliva. For the healthy and diabetes samples, the recovery values for MG were 95–103 % and 102–111 %, respectively. This approach is truly noninvasive and circumvents the discomforts associated with the traditional modalities.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100444"},"PeriodicalIF":10.61,"publicationDate":"2024-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000086/pdfft?md5=674c68aedf012f0c96e8069884f4477f&pid=1-s2.0-S2590137024000086-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139586908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern technology advances of Pseudomonas aeruginosa based biosensor approach","authors":"Dhanendiren Narayanasamy ,&nbsp;Safura Taufik ,&nbsp;Ahmad Farid Mohd Azmi ,&nbsp;Siti Aminah Mohd Nor ,&nbsp;Jahwarhar Izuan Abdul Rashid","doi":"10.1016/j.biosx.2024.100441","DOIUrl":"10.1016/j.biosx.2024.100441","url":null,"abstract":"<div><p>The threat posed by microbial infections to human health remains very significant, particularly in considering the increasing mechanisms of antibiotic resistance. <em>Pseudomonas aeruginosa</em> is known for its antibiotic resistance and is linked to serious conditions like ventilator-associated pneumonia and cystic fibrosis. Rapid infection elimination relies on identifying harmful bacteria and preventing colonization. Due to the redox-active nature of <em>P. aeruginosa</em> biomarkers metabolites, several electrochemical sensing approaches are being evaluated as diagnostic platforms for the rapid and precise detection of <em>P. aeruginosa</em> with high sensitivity. These sensors must be developed and modified to achieve optimal selectivity, sensitivity, and biocompatibility, enabling dynamic, real-time metabolite detection. This minireview highlights recent advancements in electrochemical and optical biosensors for detecting <em>P. aeruginosa</em> biomarkers, particularly pyocyanin. It highlighted design, analytical performance, biological recognition elements, and personalized wearable electrochemical sensor devices. It concludes with the future suggestions of sensor concepts for clinical diagnostics, environmental monitoring, and food safety.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100441"},"PeriodicalIF":10.61,"publicationDate":"2024-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000050/pdfft?md5=099bb01d4140f2dbaab739e43f653384&pid=1-s2.0-S2590137024000050-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139552342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical framework for dynamic tracking of Soil Organic Matter","authors":"Vikram Narayanan Dhamu ,&nbsp;Anirban Paul ,&nbsp;Sriram Muthukumar ,&nbsp;Shalini Prasad","doi":"10.1016/j.biosx.2024.100440","DOIUrl":"10.1016/j.biosx.2024.100440","url":null,"abstract":"<div><p>Soil Health parameters serve as excellent surrogate measures towards assessing environmental quality and understanding effects of climate change mitigation via carbon sequestration. Soil Organic Matter (SOM) is a parameter that is synonymous to soil health and understanding SOM is a key metric to building and influencing good soil and agronomic practices by impacting soil aggregation and water withholding capacity. It is a vital regulator of soil nutrient cycling and uptake as well as a factor in the global carbon cycle and is hence more advantageous than just carbon monitoring. While it is understood that soil health cannot be analyzed directly, the use of an efficient indicator that can relay information about the soil physico-chemical and biological characteristics is highly desirable since it offers the ability to analyze soil information over time and build patterns in terms of geographical location.</p><p>The proposed sensing system offers an in-situ electroanalytical approach to survey various electroactive substances present in the soil matrix. Utilizing this experimental framework- A mechanism of interaction between the RTIL (Room Temperature Ionic-Liquid) modified electrode and the OM functional moieties based on hydrogen bonding and pi-pi interactions captured using electrochemical impedance spectroscopy method is utilized to build a first-of-a-kind electrochemical SOM sensor.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100440"},"PeriodicalIF":10.61,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000049/pdfft?md5=593646efa5e2764dbbad5ef991c94038&pid=1-s2.0-S2590137024000049-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139506452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced graphene oxide-gadolinium oxide-functionalized paper based immunosensor for electrochemical detection of gentamicin","authors":"Jayendra Kumar Himanshu ,&nbsp;G.B.V.S. Lakshmi ,&nbsp;Akhilesh Kumar Singh ,&nbsp;Pratima R. Solanki","doi":"10.1016/j.biosx.2024.100442","DOIUrl":"10.1016/j.biosx.2024.100442","url":null,"abstract":"<div><p>Excessive consumption of antibiotics like gentamicin (GEN) can lead to hostile effects as antibiotic resistance. Therefore, the detection is important for which, reduced graphene oxide-Gadolinium oxide nanocomposite (rGO@Gd₂O₃ NC) was composed through co-precipitation method for the detection of GEN. The structural, morphological and functional group characterizations were done using XRD, FT-IR, SEM and TEM techniques. The cyclic voltammetry (CV) showed excellent electrocatalytic activity and superior performance towards GEN detection. Through the use of GEN monoclonal antibodies (anti-GEN) on a screen-printed electrode (SPE), a very sensitive electrochemical immunosensor was fabricated. Covalent interactions were employed to construct the electrochemical immunosensor, while bovine serum albumin (BSA) was employed as a blocking agent on the anti-GEN/rGO@Gd₂O₃/SPE electrode surface. The analysis of the CV response of the BSA/anti-GEN/rGO@Gd₂O₃/SPE bioelectrode demonstrated linear detection range from 1 pM – 100 μM, along with limit of detection (LOD) of 0.424 pM and sensitivity of 44.87 μA pM^-1 cm^{− 2}. Additionally, rGO@Gd₂O₃ immunosensor, exhibited a good level of linearity with R² value of 0.978. These findings indicate the excellent potential of the rGO@Gd₂O₃ electrochemical immunosensor for accurately detecting GEN in spiked milk samples at different concentrations.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100442"},"PeriodicalIF":10.61,"publicationDate":"2024-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000062/pdfft?md5=faab388e2f48a6ab8362314cdf7a22e5&pid=1-s2.0-S2590137024000062-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139501676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collaborative biofluid analysis based multi-channel integrated wearable detection system for the monitoring of wound infection","authors":"Zetao Chen ,&nbsp;Yifan Dong ,&nbsp;Jie Fu ,&nbsp;Yongchang Bai ,&nbsp;Qiya Gao ,&nbsp;Ziyue Qin ,&nbsp;Jiawang Wang ,&nbsp;Shuang Li","doi":"10.1016/j.biosx.2024.100443","DOIUrl":"10.1016/j.biosx.2024.100443","url":null,"abstract":"<div><p>The infection monitoring of chronic wounds can effectively improve the quality of wound care. However, the widely used single variable intermittent monitoring of wound provides little available information, which leads to inaccurate diagnosis and untimely warnings. In this study, a collaborative biofluid analysis based multi-channel integrated wearable detection system was constructed for the continuous detection of analytes such as pH, uric acid (UA), and C-reactive protein (CRP) in wound exudates with time division multiplexing. Based on the functionally modification with nanomaterials, integrated screen-printed electrodes (iSPE) with three working electrodes were designed for the collaboratively analyzing of wound exudates. Through the development of integrated circuits, the multi-channel wearable detection printed circuit board was constructed. With a self-designed interface, this iSPE was stably connected to the printed circuit board and realized the detection of three targets in the range of pH 3–8, UA concentrations 5–500 μmol/L, and CRP concentrations 1–1000 ng/mL at the same time. Combined with a smartphone, these results were collaborated analyzed and transferred for health management. Therefore, this integrated wearable multi-channel detection system can provide reliable and continuous evaluations for early warning of infection and further treatment of chronic wounds.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"17 ","pages":"Article 100443"},"PeriodicalIF":10.61,"publicationDate":"2024-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000074/pdfft?md5=e28c62edd264e7acd01166e994500120&pid=1-s2.0-S2590137024000074-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139496681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of chrono-spectral gold nanoparticle growth based plasmonic biosensor platform","authors":"A. Baran Sözmen ,&nbsp;Beste Elveren ,&nbsp;Duygu Erdogan ,&nbsp;Bahadır Mezgil ,&nbsp;Yalın Bastanlar ,&nbsp;U. Hakan Yildiz ,&nbsp;Ahu Arslan Yildiz","doi":"10.1016/j.biosx.2024.100439","DOIUrl":"10.1016/j.biosx.2024.100439","url":null,"abstract":"<div><p>Plasmonic sensor platforms are designed for rapid, label-free, and real-time detection and they excel as the next generation biosensors. However, current methods such as Surface Plasmon Resonance require expertise and well-equipped laboratory facilities. Simpler methods such as Localized Surface Plasmon Resonance (LSPR) overcome those limitations, though they lack sensitivity. Hence, sensitivity enhancement plays a crucial role in the future of plasmonic sensor platforms. Herein, a refractive index (RI) sensitivity enhancement methodology is reported utilizing growth of gold nanoparticles (GNPs) on solid support and it is backed up with artificial neural network (ANN) analysis. Sensor platform fabrication was initiated with GNP immobilization onto solid support; immobilized GNPs were then used as seeds for chrono-spectral growth, which was carried out using NH₂OH at varied incubation times. The response to RI change of the platform was investigated with varied concentrations of sucrose and ethanol. The detection of bacteria <em>E.coli</em> BL21 was carried out for validation as a model microorganism and results showed that detection was possible at 10² CFU/ml. The data acquired by spectrophotometric measurements were analyzed by ANN and bacteria classification with percentage error rates near 0% was achieved. The proposed LSPR-based, label-free sensor application proved that the developed methodology promises utile sensitivity enhancement potential for similar sensor platforms.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"16 ","pages":"Article 100439"},"PeriodicalIF":10.61,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000037/pdfft?md5=f5623cb51187cb9228d8b342bd22fa6e&pid=1-s2.0-S2590137024000037-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139422271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Longitudinal monitoring of IL-6 and CRP in inflammatory bowel disease using IBD-AWARE","authors":"Robert P. Hirten ,&nbsp;Kai-Chun Lin ,&nbsp;Jessica Whang ,&nbsp;Sarah Shahub ,&nbsp;Nathan K.M. Churcher ,&nbsp;Drew Helmus ,&nbsp;Sriram Muthukumar ,&nbsp;Bruce Sands ,&nbsp;Shalini Prasad","doi":"10.1016/j.biosx.2023.100435","DOIUrl":"10.1016/j.biosx.2023.100435","url":null,"abstract":"<div><p>There are limitations to monitoring modalities for chronic inflammatory conditions, including inflammatory bowel disease (IBD). Wearable devices are scalable mobile health technology that present an opportunity to monitor markers that have been linked to worsening, chronic inflammatory conditions and enable remote monitoring. In this research article, we evaluate and demonstrate a proof-of-concept wearable device to longitudinally monitor inflammatory and immune markers linked to IBD disease activity in sweat compared to expression in serum. Sixteen participants with an IBD-related hospital admission and a C-reactive protein (CRP) &gt; 5 μg/mL were followed for up to 5 days. The sweat sensing device also known as IBD AWARE was worn to continuously measure CRP and interleukin-6 (IL-6) in the sweat of participants via electrochemical impedance spectroscopy. Serum samples were collected daily. A linear relationship between serum and sweat readings for CRP and IL-6 was demonstrated based on individual linear correlation coefficients. Pooled CRP and IL-6 serum-to-sweat ratios demonstrated improving correlation coefficients as serum cutoffs decreased. Between the first and last day of observation, significant and non-significant trends in serum CRP and IL-6 were observed in the sweat. Comparison of sweat measurements between the subjects with active IBD and 10 healthy subjects distinguished an inflamed and uninflamed state with an AUC of 0.85 (95% CI: 0.68–1.00) and a sensitivity and specificity of 82% and 70% at a CRP cutoff of 938.9 pg/mL. IBD AWARE wearable device holds promise in longitudinally monitoring individuals with IBD and other inflammatory diseases.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"16 ","pages":"Article 100435"},"PeriodicalIF":10.61,"publicationDate":"2024-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137023001383/pdfft?md5=f16ba671db8902adeb961c94e1bafa94&pid=1-s2.0-S2590137023001383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139375999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}