Biosensors and Bioelectronics: X最新文献_第2页

Integrated bioelectronics for diagnostic, therapeutic and prognostic oncology 用于肿瘤学诊断、治疗和预后的综合生物电子学

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-02-26 DOI: 10.1016/j.biosx.2026.100762

Shreya Baghel , Saloni Agarwal , Baseerat Mashqoor , Rashmi Singh , Nilesh Kumar Sharma

{"title":"Integrated bioelectronics for diagnostic, therapeutic and prognostic oncology","authors":"Shreya Baghel , Saloni Agarwal , Baseerat Mashqoor , Rashmi Singh , Nilesh Kumar Sharma","doi":"10.1016/j.biosx.2026.100762","DOIUrl":"10.1016/j.biosx.2026.100762","url":null,"abstract":"<div><div>Cancer remains a major global health challenge, marked by complexity, heterogeneity, and resistance to conventional therapies. The evolving field of bioelectronics, an interdisciplinary domain at the interface of biology and electronics, offers promising innovations in cancer diagnosis, treatment, monitoring, and prognosis. Recent advances include the development of wearable and implantable devices, microfluidic chips, and bio-hybrid technologies, such as biobots and lab-on-a-chip platforms. These tools support non-invasive diagnostics, personalized treatment strategies, and continuous patient monitoring with improved sensitivity and specificity. Furthermore, bioelectronic systems enhance drug delivery and immunotherapy by leveraging electric fields, nanoparticles, and programmable electronic interfaces. Integration with artificial intelligence and tissue engineering further augments their potential. While significant progress has been made, challenges such as biocompatibility, long-term stability, and clinical scalability remain. Nonetheless, bioelectronics represents a transformative frontier in oncology, offering a futuristic pathway toward smarter, more adaptive, and patient-centric cancer care. This review examines how bioelectronic devices, particularly biosensors, integrate biological recognition elements with electronic systems to enable the real-time detection of cancer biomarkers, facilitating early and precise intervention.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100762"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OCTANE- Oral Cytokine Testing Apparatus for Non-invasive Evaluation 用于无创评估的口服细胞因子检测仪

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1016/j.biosx.2026.100746

Nathan Kodjo Mintah Churcher , Fatima Zia Rizvi , Salsabil Aziz , Sasya Madhurantakam , Crisvin Kadambathil , Vikram Dhamu , Shalini Prasad

{"title":"OCTANE- Oral Cytokine Testing Apparatus for Non-invasive Evaluation","authors":"Nathan Kodjo Mintah Churcher , Fatima Zia Rizvi , Salsabil Aziz , Sasya Madhurantakam , Crisvin Kadambathil , Vikram Dhamu , Shalini Prasad","doi":"10.1016/j.biosx.2026.100746","DOIUrl":"10.1016/j.biosx.2026.100746","url":null,"abstract":"<div><div>Cytokines such as interleukin-13 (IL-13) and thymic stromal lymphopoietin (TSLP) are pivotal biomarkers in type 2 inflammatory diseases, including asthma and allergic airway disorders. Current cytokine quantification methods are limited by centralized laboratory requirements, large sample volumes, and extended assay times, limiting their utility for point-of-care (POC) diagnostics. Here, we present OCTANE (Oral Cytokine Testing Apparatus for Non-invasive Evaluation), a portable multiplexed electrochemical biosensing platform employing miniaturized HexaPie.m electrodes and non-faradaic electrochemical impedance spectroscopy (nF-EIS) for rapid, label-free detection of IL-13 and TSLP in human saliva. Finite Element Analysis and empirical testing demonstrated that electrode miniaturization enhances sensitivity by increasing baseline impedance and electric field concentration. The OCTANE device integrates automated multiplexing and flexible communication interfaces within a compact form factor, facilitating reliable biomarker quantification with reduced user intervention. The OCTANE device's performance was rigorously evaluated using calibration curves that demonstrated high linearity and specificity, and spike-and-recovery assays that confirmed accurate quantification in complex saliva matrices. Comparative testing with benchtop potentiostats yielded a strong correlation (R<sup>2</sup> > 0.98) and negligible bias, validating the analytical precision of the OCTANE device. The modular design of the OCTANE device further enables adaptability to diverse biomarker panels, positioning it as a next-generation tool for point-of-care immunodiagnostics.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100746"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrophoretically deposited carbon nanotube-based reagent-free aptasensor for point of care detection of Pseudomonas aeruginosa in urine samples 电泳沉积的碳纳米管基无试剂适体传感器用于尿样中铜绿假单胞菌的定点检测

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1016/j.biosx.2026.100745

Boobalan Thulasinathan , Sethupathi Murugan , Rajasekaran Neelamegam , Pandiaraj Manickam

{"title":"Electrophoretically deposited carbon nanotube-based reagent-free aptasensor for point of care detection of Pseudomonas aeruginosa in urine samples","authors":"Boobalan Thulasinathan , Sethupathi Murugan , Rajasekaran Neelamegam , Pandiaraj Manickam","doi":"10.1016/j.biosx.2026.100745","DOIUrl":"10.1016/j.biosx.2026.100745","url":null,"abstract":"<div><div>Increasing incidences of pathogenic bacterial outbreaks, together with growing environmental and public health concerns, have accelerated the development of electrochemical point-of-care (POC) diagnostic systems that are rapid, reliable, and cost-effective, offering high specificity and sensitivity for the on-site detection of bacterial pathogens. <em>Pseudomonas aeruginosa</em> is a ubiquitous opportunistic pathogen and it is also causing nosocomial infections and poses significant challenges to public health. In this study, we demonstrate a reagent-free electrochemical aptasensor for the highly sensitive detection of <em>Pseudomonas aeruginosa</em>. The redox label methylene blue (MB) was covalently linked to an amino-modified aptamer, which binds selectively to the target bacteria, enabling electron transfer quantification through square-wave voltammetry (SWV). Screen-printed carbon electrodes (SPCEs) were modified with carboxylated multi-walled carbon nanotubes (c-MWCNTs) via an electrophoretic deposition technique to enhance surface conductivity and signal response. The developed aptasensor exhibited a linear dynamic range (LDR) from 1 × 10<sup>1</sup> to 1 × 10<sup>7</sup> colony-forming units (CFU)/mL, with an impressive limit of detection (LOD) of 1 CFU mL<sup>−1</sup>. The reagent-free electrochemical aptasensor was successfully validated using undiluted urine samples spiked with <em>Pseudomonas aeruginosa</em>. Overall, the fabricated aptasensor demonstrates strong potential as a powerful diagnostic tool for the rapid and sensitive detection of <em>Pseudomonas aeruginosa</em> in clinical and environmental samples.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100745"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agarase-based bacterial biosensor for detection of cadmium in environmental samples 基于琼脂酶的细菌生物传感器用于环境样品中镉的检测

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-01-30 DOI: 10.1016/j.biosx.2026.100750

Rajkumar Prabhakaran , Ramprasath Tharmarajan , Selvam Govindan Sadasivam

{"title":"Agarase-based bacterial biosensor for detection of cadmium in environmental samples","authors":"Rajkumar Prabhakaran , Ramprasath Tharmarajan , Selvam Govindan Sadasivam","doi":"10.1016/j.biosx.2026.100750","DOIUrl":"10.1016/j.biosx.2026.100750","url":null,"abstract":"<div><div>Cadmium (Cd) contamination, which requires sensitive and cost-effective detection methods for environmental monitoring. In this study, the Cd-responsive promoter P<sub><em>cadR</em></sub> from <em>P. aeruginosa</em> strains RGS16 and RGS23 was functionally characterized and compared with <em>Escherichia coli</em> strains carrying pUCP-P<sub><em>cadR</em></sub>-<em>gfp</em><sup><em>mut2</em></sup>. The Cd sensing ability of this construct was standardized and analyzed by fluorescence microscopic imaging, high-throughput cell imaging, flow cytometric, fluorometric, and colorimetric analysis. Promoter activity was first evaluated using a GFP-based reporter, revealing significantly higher GFP expression in <em>P. aeruginosa</em> strains and a linear response to CdCl<sub>2</sub> concentrations between 56.2 and 562 ppm (0.5–5 mM). To extend biosensor applicability, a 938 bp β-agarase (<em>dagA</em>) reporter gene was fused downstream of the P<sub><em>cadR</em></sub> promoter, generating pUCP-P<sub><em>cadR</em></sub>-<em>dagA</em>39 and pUCP-P<sub><em>cadR</em></sub>-<em>dagA</em>40 constructs. The engineered <em>P. aeruginosa</em> BC15 strain exhibited a broad linear detection range from 10 ppb to 500 ppm CdCl<sub>2</sub>, with a high correlation coefficient (R<sup>2</sup> = 0.9851). Furthermore, immobilized <em>Pa-CadBacS</em>-GSR39 and GSR40 biosensors showed strong linear responses in both ppb and ppm ranges (R<sup>2</sup> = 0.94–0.963). Cd concentrations measured using the biosensor system correlated with ICP-MS analysis (R<sup>2</sup> = 0.967), confirming its accuracy. These results demonstrate that the P<sub><em>cadR</em></sub>-based whole-cell biosensor is a low-cost, sensitive, and reliable platform for quantitative Cd detection across environmentally relevant concentrations.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100750"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of an electrically conductive film and stimulation of neurons in a microfluidic platform integrated with ITO-coated PET electrodes 导电膜的合成及在集成ito涂层PET电极的微流控平台中刺激神经元

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-01-21 DOI: 10.1016/j.biosx.2026.100744

Reyhan Coban , Aysel Saskara , Utku Devamoglu , Ozlem Yesil-Celiktas

{"title":"Synthesis of an electrically conductive film and stimulation of neurons in a microfluidic platform integrated with ITO-coated PET electrodes","authors":"Reyhan Coban , Aysel Saskara , Utku Devamoglu , Ozlem Yesil-Celiktas","doi":"10.1016/j.biosx.2026.100744","DOIUrl":"10.1016/j.biosx.2026.100744","url":null,"abstract":"<div><div>Hybrid polymer materials are engineered by coupling distinct structural and functional domains, enabling distinctive properties unattainable in single-component systems. Hybrid polymer films stand out as promising materials that integrate physicochemical functionality with biological responses for tissue engineering applications. Mechanically robust, biocompatible polymer with extensive properties, polyvinylidene fluoride (PVDF) represents a sought-after polymer for tissue engineering applications. Combining it with non-toxic conductive nanomaterials offers a promising technique to enhance conductivity and enable electrical stimulation for regulating the behavior of neuronal cells. Carbon nanofibers (CNFs) are among the most promising candidates for this purpose. In this study, electrically conductive PVDF/CNF composite films were synthesized under optimized conditions, characterized and evaluated for adaptability in neuronal cell culture for electrical stimulation applications. Optimized films exhibited fibrillar networks, as confirmed by multiple analyses. While CNFs act as the primary determinant of electrical conductivity, PVDF contributes to the mechanical robustness of the composite. Various coating strategies were applied and optimized to promote NSC-34 cell adhesion, proliferation, and directional orientation. Afterwards, functional evaluation using an Arduino-based ITO-integrated microfluidic platform (μ-platform) showed that AC and DC modes successfully enabled electrical stimulation. These results indicate that surface topography, protein functionalization, and optimized electrical properties support neuronal cell attachment and viability, thus providing a versatile platform for neuronal stimulation applications.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100744"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146006592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A reliable high-throughput OECT-based bioelectronic system enabling pan-specific vesicle quantification 一种可靠的高通量基于oect的生物电子系统，可实现泛特异性囊泡定量

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-02-06 DOI: 10.1016/j.biosx.2026.100755

Giulia Frusconi , Angelo Musicò , Andrea Zendrini , Zsolt M. Kovács-Vajna , Lucia Paolini , Paolo Bergese , Fabrizio Torricelli

{"title":"A reliable high-throughput OECT-based bioelectronic system enabling pan-specific vesicle quantification","authors":"Giulia Frusconi , Angelo Musicò , Andrea Zendrini , Zsolt M. Kovács-Vajna , Lucia Paolini , Paolo Bergese , Fabrizio Torricelli","doi":"10.1016/j.biosx.2026.100755","DOIUrl":"10.1016/j.biosx.2026.100755","url":null,"abstract":"<div><div>Next-generation extracellular vesicle (EV) technologies are transforming biomanufacturing and functional product development. Quantifying EVs based on their membrane protein content is crucial for ensuring consistent yield, composition, and functional performance in manufacturing pipelines. Existing methods measure EV size and concentration but provide no information on membrane protein presence, whereas immunoaffinity approaches depend on specific markers that may be inconsistently expressed due to EV heterogeneity. Here we demonstrate a reliable bioelectronic system for pan-specific, high-throughput total EV quantification, achieving 12 independent parallel analysis in less than 15 min, with false-positive and false-negative rates below 1%. The system features multiple channel-functionalized arrays of organic electrochemical transistors (OECTs) in a 3 × 4 matrix configuration. Click chemistry enables real-time detection of EVs with membrane proteins, with a limit of identification of 6 × 10<sup>6</sup> particles ml<sup>−1</sup> and a dynamic range spanning three orders of magnitude. By integrating functionalized OECT arrays with 3D-printed fluidic devices into a low-cost, disposable cartridge operated by a compact silicon-based electronic reader, the system enables rapid, reliable, and pan-specific total EV quantification in a user-friendly format, opening opportunities for in-line quality control and scalable implementation in biomanufacturing and pharmaceutical processes.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100755"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent developments and innovations in nanomaterials-supported biosensors for health monitoring 纳米材料支持的健康监测生物传感器的最新发展和创新

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-02-16 DOI: 10.1016/j.biosx.2026.100761

Ahmed Isa , Ahmet Cetinkaya , Sibel A. Ozkan

{"title":"Recent developments and innovations in nanomaterials-supported biosensors for health monitoring","authors":"Ahmed Isa , Ahmet Cetinkaya , Sibel A. Ozkan","doi":"10.1016/j.biosx.2026.100761","DOIUrl":"10.1016/j.biosx.2026.100761","url":null,"abstract":"<div><div>The increased access to nanoscaled materials has allowed an explosive growth in various fields, including healthcare and biosensing platforms, as the integration of nanomaterials into these systems allows the design of diagnostic tools that are more sensitive, selective, and capable of delivering real-time results. This review, covering various up-to-date resources between the years 2020-2025, expands on the types and functions of nanomaterials used in health monitoring and on progress in nanomaterial-based biosensors, focusing on their classification, synthesis, functionalization, and effects on generated signals. Furthermore, the review also organizes biosensors according to the biorecognition elements while discussing developing technologies like CRISPR/Cas biosensors, and finally examines the various applications in health-related diagnostics. Various studies, which include wearable and patient-friendly devices, were included, and the movement toward point-of-care testing and continuous health tracking was demonstrated. By connecting these technological advances to the needs of both clinical practice and everyday health monitoring, the review underscores the growing importance of nanomaterials in the fabrication of biosensors for personalized medicine and their steady path toward broader commercialization.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100761"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interactions of ferrocenyl triphenylamine compounds with biomimetic membranes studied by nanoplasmonic sensing and micro differential scanning calorimetry 纳米等离子体传感和微差扫描量热法研究了二茂铁基三苯胺化合物与仿生膜的相互作用

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-02-11 DOI: 10.1016/j.biosx.2026.100756

Shishir Jaikishan , Hassan Abul-Futouh , Atemni Ibrahim , Rosa Verdú Revilla , Rui Chen , Ibrahim Basma , Dumidu Perera , Wolfgang Weigand , Susanne K. Wiedmer

{"title":"Interactions of ferrocenyl triphenylamine compounds with biomimetic membranes studied by nanoplasmonic sensing and micro differential scanning calorimetry","authors":"Shishir Jaikishan , Hassan Abul-Futouh , Atemni Ibrahim , Rosa Verdú Revilla , Rui Chen , Ibrahim Basma , Dumidu Perera , Wolfgang Weigand , Susanne K. Wiedmer","doi":"10.1016/j.biosx.2026.100756","DOIUrl":"10.1016/j.biosx.2026.100756","url":null,"abstract":"<div><div>Ferrocene is a versatile organometallic compound consisting of two cyclopentadienyl rings bonded to an iron atom. The introduction of various functional groups into the cyclopentadienyl rings enhances its chemical, physical, and electronic properties. Such modifications are extensively applied in the field of catalysis, material sciences, medicine, drug delivery, chemical, and bio-sensing. Similarly, the ferrocene moiety can be linked to bulky fluoro-chromogenic units like triphenylamine for various biomedical imaging and biosensing applications, due to the fluorescent properties of triphenylamine. In this work, triphenylamine is bonded to a ferrocene moiety via a ketone group, resulting into a ‘hybrid novel material’ which may exhibit synergistic properties that neither molecule could achieve alone. For extensive characterization, three additional novel derivatives, namely, ferrocenyl triphenylamine thioketone, ferrocenyl triphenylamine thiophene ketone, and ferrocenyl triphenylamine thiophene thioketone were synthesized, and included in the study. The hydrophobicity and their interactions with lipid membranes were investigated using nanoplasmonic sensing, micro differential scanning calorimetry, and fluorescence spectroscopy.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100756"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147384968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Next-generation platforms for NADH monitoring 下一代NADH监测平台

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-02-03 DOI: 10.1016/j.biosx.2026.100753

Xuchao Wang , Mingyue Gong , Xiaoxiao Men , Haobin Chen

{"title":"Next-generation platforms for NADH monitoring","authors":"Xuchao Wang , Mingyue Gong , Xiaoxiao Men , Haobin Chen","doi":"10.1016/j.biosx.2026.100753","DOIUrl":"10.1016/j.biosx.2026.100753","url":null,"abstract":"<div><div>Reduced nicotinamide adenine dinucleotide (NADH) serves as a central redox coenzyme and a critical indicator of cellular metabolic status. Its dynamic fluctuations are intimately linked to the pathogenesis of a wide range of diseases, making its precise, real-time monitoring imperative for advancing diagnostics, therapeutic monitoring, and fundamental metabolic research. While conventional analytical techniques are constrained by their operational complexity and lack of portability, the emergence of portable biosensors offers a paradigm-shifting solution. This review comprehensively synthesizes recent progress in three primary categories of portable NADH biosensors: optical (including fluorescence, colorimetric, and surface-enhanced Raman scattering), electrochemical, and synthetic biology-based platforms. We provide a critical analysis of their operational mechanisms, performance benchmarks in terms of sensitivity, selectivity, and response time, and their application in both ex vivo and in vivo settings. Despite significant advancements, the clinical translation of these technologies faces persistent challenges, such as interference in complex biological matrices, the need for long-term stability, and seamless integration into user-friendly, multiplexed devices. Looking forward, we posit that the convergence of advanced nanomaterials, innovative biorecognition elements, and machine learning analytics will be pivotal in overcoming these hurdles. The ultimate maturation of these portable platforms is poised to usher in an era of real-time metabolomic medicine, transforming NADH from a biochemical analyte into a dynamic, actionable biomarker for precision health.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100753"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-monitoring of β-hydroxybutyrate and glucose via a microneedles-based biosensor for personalized nutrition 基于微针的个性化营养生物传感器对β-羟基丁酸盐和葡萄糖的双重监测

IF 10.61

Biosensors and Bioelectronics: X Pub Date : 2026-05-01 Epub Date: 2026-01-29 DOI: 10.1016/j.biosx.2026.100747

Cristina Tortolini , Massimiliano Caprio , Anisa Degjoni , Andrea Lenzi , Andrea M. Isidori , Riccarda Antiochia

{"title":"Dual-monitoring of β-hydroxybutyrate and glucose via a microneedles-based biosensor for personalized nutrition","authors":"Cristina Tortolini , Massimiliano Caprio , Anisa Degjoni , Andrea Lenzi , Andrea M. Isidori , Riccarda Antiochia","doi":"10.1016/j.biosx.2026.100747","DOIUrl":"10.1016/j.biosx.2026.100747","url":null,"abstract":"<div><div>Personalized nutrition has rapidly emerged as a highly interdisciplinary field dedicated to preventing and managing diseases by tailoring nutrition based on the specific requirements and unique metabolic profiles of each individual. This approach addresses the profound heterogeneity in how individuals respond to diet, considering factors such as genetics, lifestyle, and microbiome composition. The successful realization of effective personalized nutrition requires the development and synergy of diverse sensor modalities. Microneedles-based biosensors are excellent candidates as they are capable of continuously tracking temporal bioanalytes variations in the interstitial fluid (ISF), following the intake of food and supplements. In this work, we present a wearable minimally-invasive electrochemical biosensor for dual-monitoring of β-hydroxybutyrate (β-HB) and glucose in ISF, based on a gold microneedles array with two working electrodes. Functionalization with electropolymerized poly-methylene blue (poly-MB), β-hydroxybutyrate dehydrogenase (3-HBDH) with nicotinamide adenine dinucleotide oxidized form (NAD<sup>+</sup>) and chitosan nanoparticles (ChitNPs) (working electrode 1), and FAD-glucose-dehydrogenase (FADGDH) (working electrode 2), enabled the dual-monitoring of β-HB and glucose, with linear range in the clinically relevant range of both analytes, high sensitivity, stability, and selectivity, avoiding cross-talk between the two closely modified working electrodes. An outer poly(vinyl) chloride (PVC) diffusion-limiting membrane was used to protect the modified microneedles array. The developed biosensor was tested in artificial ISF, in a phantom gel skin-mimicking model as well as in human ISF. This pilot study involved three human healthy subjects before and after taking supplements, and the results, compared to those obtained with a commercial ketone/glucose meter, showed excellent agreement.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"29 ","pages":"Article 100747"},"PeriodicalIF":10.61,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146170625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0