Muge Yucel , Ahmet Önder , Tolunay Kurt , Berfin Keles , Merve Beyaz , Yaren Karadağ , İrem Yaşyerli , A. Irem Celik , Fatih Sema , Senem Tetik , Sanem Dinçkal , Soner Karabacak , Palaniappan Alagappan , Bo Liedberg , Umit Hakan Yildiz
{"title":"Digital sensing technologies in cancer care: A new era in early detection and personalized diagnosis","authors":"Muge Yucel , Ahmet Önder , Tolunay Kurt , Berfin Keles , Merve Beyaz , Yaren Karadağ , İrem Yaşyerli , A. Irem Celik , Fatih Sema , Senem Tetik , Sanem Dinçkal , Soner Karabacak , Palaniappan Alagappan , Bo Liedberg , Umit Hakan Yildiz","doi":"10.1016/j.biosx.2025.100651","DOIUrl":"10.1016/j.biosx.2025.100651","url":null,"abstract":"<div><div>Digital sensor platforms are systems that integrate sensors with digital technology, which revolutionize data collection, processing, and transmission for enabling real-time, high-precision and automated diagnostics. These platforms often serve as the backbone of modern monitoring systems, enabling real-time data acquisition and analysis for a wide range of applications. Recent advancements in digital sensor platforms have paved the way for transformative innovations in cancer diagnosis. These cutting-edge technologies offer unprecedented opportunities to facilitate early detection, improve diagnostic accuracy, and personalize treatment methods. This review explores the landscape of digital sensor platforms in the context of cancer diagnosis, providing an overview of their principles, functionalities, and clinical applications. The review further illustrates that biosensors, lab-on-a-chip (LOC) devices and wearable sensors have leveraged on nanotechnology, biorecognition materials and artificial intelligence (AI) for revolutionizing cancer diagnosis. It consolidates the recent advances in digital sensor platforms for cancer diagnosis and the associated critical challenges, such as regulatory concerns, standardization, and ethical considerations. Further, the review summarizes the feasibility for the integration of digital sensor platforms with routine clinical practices for the development of efficient cancer diagnosis and treatment methods.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100651"},"PeriodicalIF":10.61,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jithin Thomas Chacko , K. Niyas , Anson T. Mathew , P Abdul Rasheed
{"title":"Nanostructure modified carbon thread as wearable patch for enzymatic and non-invasive monitoring of glucose from human samples","authors":"Jithin Thomas Chacko , K. Niyas , Anson T. Mathew , P Abdul Rasheed","doi":"10.1016/j.biosx.2025.100653","DOIUrl":"10.1016/j.biosx.2025.100653","url":null,"abstract":"<div><div>Traditional blood-based glucose detection methods are invasive, inconvenient, and unsuitable for continuous monitoring, highlighting the critical need for a non-invasive alternative. To address this urgent need, we developed a non-invasive and wearable electrochemical glucose sensor patch based on a platinum-copper (Pt-Cu) nanocomposite electrodeposited on carbon cloth thread (CCT) electrode after functionalizing with an electropolymerized polyphenol (Pphe)-glucose oxidase (GOx) film. The immobilization of GOx via the formation of a polyphenol film was done by electropolymerization of phenol in presence of GOx. The detection capability of the developed sensor was evaluated by chronoamperometry analysis and found that the sensor is able to detect glucose with wide linear detection range from 100 to 1500 μM with an ultra-low limit of detection (LOD) of 31.1 μM and 64.4 μM for artificial sweat and urine respectively. In addition, the developed sensor is found to be reproducible, repeatable and highly selective in presence of common interfering molecules present in the human samples. Furthermore, the developed sensor was seamlessly integrated into a wearable prototype device on a commercial bandage with custom made potentiostat circuit called Nanopot. The developed wearable prototype sensor system also exhibits a wide linear detection range from 300 to 1500 μM and a promising LOD of 25.8 μM. These results further validate the reliability, consistency, and practical applicability of the sensor system with a promising potential to address the urgent demand for non-invasive, painless and user-friendly diabetes management.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100653"},"PeriodicalIF":10.61,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kazi Mustafizur Rahman, Md Mushfiqur Rahman, Sadia Islam, Hasib Md Abid Bin Farid, Md Faysal Nayan
{"title":"Design and analysis of a magneto-resistance-based device to mitigate risks from high magnetic field exposure","authors":"Kazi Mustafizur Rahman, Md Mushfiqur Rahman, Sadia Islam, Hasib Md Abid Bin Farid, Md Faysal Nayan","doi":"10.1016/j.biosx.2025.100654","DOIUrl":"10.1016/j.biosx.2025.100654","url":null,"abstract":"<div><div>The motivation is to develop a device for pacemaker-implanted patients that would automatically alert them in an intense magnetic field. Moreover, the employees working near any strong magnetic environment would benefit by avoiding high exposure. This research delves into a comprehensive process for the implementation and characterization of such a wearable based on the magnetoresistance effect, which is a function of the magnetic field. The program executes on the Arduino IDE platform. Samples are taken for varying magnetic flux density along each axis, for changes in distance of 2.5 mm. The calculations take place accordingly and provide outputs in microtesla units. Subsequently, the device is analyzed by plotting the responses, and it also helps to understand the working procedure. For a certain axis, the magnetic field is generally stronger than others. The goal is to determine the highest absolute value at any instance, including the Earth's geomagnetic field of 22–67 μT. Regulatory standards are followed to divide the magnetic flux density into four states: power saver (below 150 μT), safe (150–500 μT), unsafe (500–750 μT), and danger (over 750 μT). These values consist of ±20 μT error, which is quite insignificant. Depending on the state, the novel device generates different warning signals to mitigate risk from magnetic fields. From the error bar plot, it is realized that the percentage of error decreases while calculating higher magnetic flux. The errors could be reduced remarkably by ensuring better calibration and compensation techniques in the future.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100654"},"PeriodicalIF":10.61,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144522769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Talia Tene , Marco Guevara , Isaías Caicedo , Jose Luis Granizo Jara , Myrian Borja , Lala Gahramanli , Cristian Vacacela Gomez , Stefano Bellucci
{"title":"MoS2-Si3N4-based SPR biosensor for the detection of malaria at different stages: A theoretical insight","authors":"Talia Tene , Marco Guevara , Isaías Caicedo , Jose Luis Granizo Jara , Myrian Borja , Lala Gahramanli , Cristian Vacacela Gomez , Stefano Bellucci","doi":"10.1016/j.biosx.2025.100655","DOIUrl":"10.1016/j.biosx.2025.100655","url":null,"abstract":"<div><div>The accurate differentiation of malaria stages is essential for effective treatment and epidemiological control. This work presents a theoretical analysis of a multilayer surface plasmon resonance (SPR) biosensor for malaria stage detection based on refractive index variations of infected red blood cells. The sensor combines silver, silicon nitride (Si<sub>3</sub>N<sub>4</sub>), monolayer molybdenum disulfide (MoS<sub>2</sub>), and thiol-functionalized single-stranded DNA (ssDNA) on a BK7 prism. Using the transfer matrix method (TMM), we evaluate the sensor performance across Ring, Trophozoite, and Schizont stages through sensitivity, full width at half maximum (FWHM), quality factor (QF), detection accuracy (DA), limit of detection (LoD), and comprehensive sensitivity factor (CSF). The optimized configuration achieves angular sensitivities of 318.2 (Ring), 268.9 (Trophozoite), and 244.8 (Schizont) °/RIU, demonstrating clear stage discrimination and competitive performance compared to reported multilayer SPR sensors. The layered architecture was designed with experimentally accessible materials and configurations, supporting future translation to stage-specific diagnostic platforms.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100655"},"PeriodicalIF":10.61,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Smartphone-based colorimetric detection of urinary glucose using a novel green and red/green approach","authors":"Tileshwar Sahare, Simran Rana, Badri Narayana Sahoo, Milan Khanda, Abhijeet Joshi","doi":"10.1016/j.biosx.2025.100650","DOIUrl":"10.1016/j.biosx.2025.100650","url":null,"abstract":"<div><div>Urine dipsticks are commonly used to detect pathological changes in urine due to their low cost and easy operation, having glucose parameter to detect glucosuria. Despite their advantages, the urine dipstick is ineffective in the early detection of diabetes due to its limitations in the detection of glucose below 100 mg/dl. Here, we present a novel, cost-effective ambient ring light-based smartphone platform to accurately estimate urinary glucose concentrations in lower points utilizing glucose sensors obtained from commercially available urine dipsticks (Mission® and Recombigen™). Glucose sensors changed color from green to brown with the increasing glucose concentration, resulting in a response time of 5 min. In this study, smartphone was used to capture images and analyze Red-Green-Blue (RGB) profiles, extracted with the help of an open-source smartphone-android-based Color Picker app. G intensity was determined to be inversely proportional and R/G intensity to be directly proportional to the increasing glucose concentration. LOD was found to be 6.2 and 24 mg/dl for Mission® glucose sensors with G and R/G respectively. On the other hand, Recombigen™ dipstick glucose sensors, LOD was evaluated as 5.8 and 16 mg/dl with G and R/G respectively. This smartphone-based assessment of glucose using dipsticks can be beneficial in the current screening and diagnostics sector of earlier detection of glucose in urine.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100650"},"PeriodicalIF":10.61,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144502126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"β-Cyclodextrin-conjugated butein as a selective electrochemical molecular trafficking system for bilastine","authors":"Ramya Kanagaraj , Shanmugam SenthilKumar , Gururaja Perumal Pazhani , Murugan Veerapandian","doi":"10.1016/j.biosx.2025.100647","DOIUrl":"10.1016/j.biosx.2025.100647","url":null,"abstract":"<div><div>Simple method for monitoring the therapeutic efficient concentration of anti-histamine drug, bilastine (BIL), is essential for rationalizing allergy and respiratory therapy. Herein, an external redox-mediator free molecular trafficking approach was developed using conjugated oligosaccharide-polyphenol system, β-cyclodextrin-butein (BCD-Bt). Mechanism behind the molecular loading of BIL within the hydrophobic cavity of BCD simultaneously enabling the redox-active signal transduction from the conjugated Bt are explored using cyclic and differential pulse voltammetric analyses. BCD-Bt sensor platform exemplified the selective loading of analyte BIL with a reliable detection linearity (nm to μM, <em>R</em><sup><em>2</em></sup> = 0.981) and limit feasible for salivary therapeutic drug monitoring. Synergistic optical absorbance and emission-based molecular trafficking test further complements the multi-modal functionality of the demonstrated BCD-Bt in other similar molecular assays.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100647"},"PeriodicalIF":10.61,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antonio Carcaterra, Nicola Roveri, Silvia Milana, Gianluca Pepe
{"title":"A micro resonating motor based on neuron action potential","authors":"Antonio Carcaterra, Nicola Roveri, Silvia Milana, Gianluca Pepe","doi":"10.1016/j.biosx.2025.100646","DOIUrl":"10.1016/j.biosx.2025.100646","url":null,"abstract":"<div><div>This paper introduces a novel bioelectromechanical device converting the electrochemical potential energy of excitable cells into mechanical work by coupling the Hodgkin–Huxley (HH) neuronal model response to a mechanical resonator. Addressing key challenges in bioelectromechanical systems, including biocompatibility, miniaturization, and efficient energy conversion, the device leverages the membrane potentials of biological cells to drive mechanical oscillations within microelectromechanical systems (MEMS). Through a combination of numerical simulations and theoretical analyses, it is demonstrated that the coupled HH–resonator system achieves stable limit cycles and significant mechanical displacements via parametric amplification. This amplification arises from the nonlinear capacitive coupling, which leads to Mathieu-like equations governing the system's dynamics, thereby enabling large oscillations from relatively small voltage inputs. Such parametric resonance is critical for the device's ability to sustain oscillatory motion, making it highly suitable for integration into compact and implantable MEMS applications. Potential applications include implantable sensors and actuators for real-time physiological monitoring, and advanced micro-scale systems that benefit from biologically sourced energy. The findings underscore the promise of bioelectromechanical systems in advancing biomedical and microengineering technologies, paving the way for innovative solutions in personalized medicine, bio-robotics, and beyond.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100646"},"PeriodicalIF":10.61,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144314381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Saroglia , S. Marocco , S. Nicoloso , S. Diana , M. Tozzi , I. Stefanini
{"title":"Current state of the art on implantable sensors for continuous post-EVAR surveillance of abdominal aortic aneurysms: A comprehensive review","authors":"G. Saroglia , S. Marocco , S. Nicoloso , S. Diana , M. Tozzi , I. Stefanini","doi":"10.1016/j.biosx.2025.100645","DOIUrl":"10.1016/j.biosx.2025.100645","url":null,"abstract":"<div><div>Post-endovascular aneurysm repair (EVAR) surveillance currently relies on routine imaging techniques such as computed tomography angiography and magnetic resonance angiography, which expose patients to radiation, nephrotoxic contrast agents, and place logistical burdens on healthcare systems. This review critically examines the potential of implantable sensors, particularly pressure-monitoring systems, to provide continuous, minimally invasive post-EVAR surveillance. We present an overview of the biological and biomechanical rationale for monitoring aneurysm sac pressurization, analyze existing sensor technologies including EndoSure® and ImPressure®, and discuss technical challenges such as wireless telemetry, biocompatibility, signal attenuation, and power management. Regulatory milestones and alignment with ESVS and SVS surveillance guidelines are reviewed, along with the current evidence gap between pressure trends and clinical outcomes such as reintervention or rupture. While technical feasibility has been demonstrated in controlled settings, no studies to date have shown that pressure monitoring improves long-term outcomes or guides decision-making independently of imaging. The limitations of pressure-based monitoring are discussed alongside emerging sensor designs that integrate pressure, morphological, and biochemical sensing. This review concludes that implantable pressure sensors remain investigational tools whose clinical adoption will depend on future trials demonstrating diagnostic accuracy, patient acceptability, cost-effectiveness, and outcome benefit.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100645"},"PeriodicalIF":10.61,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A versatile ITO electrode platform for studying neuronal differentiation and migration under electrical stimulation","authors":"Suthiwan Udomrat , Supeecha Kumkate , Permphan Dharmasaroja , Theeraporn Puntheeranurak , Tanakorn Osotchan","doi":"10.1016/j.biosx.2025.100637","DOIUrl":"10.1016/j.biosx.2025.100637","url":null,"abstract":"<div><div>The electrical effects on cells, particularly neurons, are extensively studied to understand cellular functions. Various culture platforms have been developed to investigate cellular responses to electrical stimulation. In this study, planar indium tin oxide (ITO) interdigitated electrodes (IDEs) with conventional and circular designs were fabricated and utilized as both cell culture platforms and stimulation electrodes for experiments up to ten days. To enhance cell adhesion, fetal bovine serum (FBS) was applied to the electrode surface and then removed, facilitating cell culture. The SH-SY5Y cell line was cultured on the platform to assess adhesion and growth. Steady-state direct-current electric field stimulation (DCEFS) at 150 V/m or 300 V/m was applied at specific intervals. Cells exposed to 150 V/m showed significant morphological changes indicative of differentiation, while those at 300 V/m migrated away, reducing intact cell numbers. Notably, circular IDEs promoted extensive neurite outgrowth compared to conventional designs. These findings highlight the potential of electrode geometry to control cell differentiation and migration, offering a versatile platform for cellular studies and tissue engineering applications.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100637"},"PeriodicalIF":10.61,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rational design of peptides for epitope imprinting of polynorepinephrine: A plasmonic and machine learning integrated approach","authors":"Davide Sestaioni , Giulia Ciacci , Andrea Barucci , Pasquale Palladino , Simona Scarano","doi":"10.1016/j.biosx.2025.100638","DOIUrl":"10.1016/j.biosx.2025.100638","url":null,"abstract":"<div><div>Molecularly Imprinted Polynorepinephrine (MIPNE) has demonstrated superior performance for mimetic receptors production, facilitating their integration into techniques like Surface Plasmon Resonance (SPR), Biomimetic Enzyme-Linked ImmunoSorbent Assay (BELISA), and Bio-Layer Interferometry (BLI). Here we developed a multiplexed Localized Surface Plasmon Resonance (LSPR) assay to face the selection of appropriate epitope sequences for protein imprinting, a critical factor in optimizing MIPNE efficiency. The plasmonic properties of gold nanoparticles formed on MIPNE were used to classify epitopes as functional (<strong><em>F</em></strong>), uncertain (<strong><em>U</em></strong>), or dysfunctional (<strong><em>D</em></strong>). Feature extraction and machine learning analysis identified key physico-chemical descriptors influencing imprinting efficiency. Subsequent SPR testing confirmed the correlation between epitope selection and receptor performance. This study provides the first systematic approach for epitope selection in MIPNE, paving the way for their improved design and application in bioanalytics and biosensing.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"26 ","pages":"Article 100638"},"PeriodicalIF":10.61,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144262701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}