Biosensors and Bioelectronics: X最新文献

{"title":"Recent advancements in nanozyme hydrogel based system for enhanced sensing applications","authors":"Simran Makkar ,&nbsp;Nikita Sarawagi ,&nbsp;Nitesh Priyadarshi ,&nbsp;Sunaina Kaul ,&nbsp;Palakjot Kour Sodhi ,&nbsp;Ajay Kumar Srivastava ,&nbsp;Nitin Kumar Singhal","doi":"10.1016/j.biosx.2024.100548","DOIUrl":"10.1016/j.biosx.2024.100548","url":null,"abstract":"<div><div>In recent years, combining hydrogels with nanozymes has shown promise as a platform for sensing applications. Nanozymes are simple, affordable, and stable alternatives that use enzyme-like capabilities. Conversely, hydrogels provide hydrated, biocompatible environments, which enhance the stability and activity of nanozymes. The most recent advancements in nanozyme-hydrogel hybrid sensing systems are examined in this study, with a focus on their enzymatic activities—peroxidase and oxidase mimetics—and how they can be used to detect a variety of analytes, such as glucose, pesticide, and many more.</div><div>In addition to simulating the biological environment, the synergistic combination of hydrogels and nanozyme improves the biosensor's sensitivity, specificity, and fast reaction times. These hybrid systems also provide an extensible framework for integrating multiple sensory modalities into a single functional device. This review highlights the groundbreaking potential and uses of hydrogel sensors based on nanozymes in environmental and medical diagnostics and potential future developments.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100548"},"PeriodicalIF":10.61,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142357829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-enhanced nanozyme sensors: Colorimetric and fluorescence sensing techniques","authors":"Tileshwar Sahare,&nbsp;Nandini Singh,&nbsp;Badri Narayana Sahoo,&nbsp;Abhijeet Joshi","doi":"10.1016/j.biosx.2024.100544","DOIUrl":"10.1016/j.biosx.2024.100544","url":null,"abstract":"<div><div>The advent of smartphone technology has changed the biosensing field, especially by integrating nanozyme-based colorimetric, fluorescence sensing strategy. This review article describes the development of smartphone-assisted nanozyme sensor technology, harboring increased sensitivity and specificity for detecting relevant markers. While mimicking natural catalytic enzymes, nanozymes show exceptional performance in a variety of analytical applications. Combining the computing power and imaging capabilities of smartphones, nanozyme based sensors offer a low-cost, user-friendly, and scalable solution for point-of-care technology. We have discussed the principles behind colorimetric and fluorescence sensing techniques, the role of nanozymes in these processes, and the methodologies for leveraging smartphone technology for data acquisition and analysis. Crucial applications in medical diagnostics, environmental monitoring, and food safety are reviewed, with a focus on recent innovations and forthcoming prospects. The convergence of nanozyme sensors and smartphone technology promises to democratize access to advanced colorimetric or fluorometric based biosensing.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100544"},"PeriodicalIF":10.61,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142324132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in gluten detection: A rapid colorimetric approach using core-satellite magnetic particles","authors":"Daniele Marra ,&nbsp;Adriano Acunzo ,&nbsp;Andrea Fulgione ,&nbsp;Maria De Luca ,&nbsp;Reynaldo Villalonga ,&nbsp;Francesco Pisani ,&nbsp;Loredana Biondi ,&nbsp;Federico Capuano ,&nbsp;Raffaele Velotta ,&nbsp;Bartolomeo Della Ventura ,&nbsp;Vincenzo Iannotti","doi":"10.1016/j.biosx.2024.100545","DOIUrl":"10.1016/j.biosx.2024.100545","url":null,"abstract":"<div><div>Monitoring gluten levels in foods labelled as gluten-free or low-gluten — i.e. containing less than 20 ppm and 100 ppm of gluten, respectively — is crucial for preventing celiac disease-related disorders. Due to their inherent complexity, the standard analytical techniques to assess the gluten content in food are not suitable for on-site measurements, whose need is widely recognized. In this context, we developed a rapid and cost-effective biosensor based on core-satellite magnetic particles (CSMPs) — magnetic cores coated with gold nanoparticles (AuNPs) — further functionalized with anti-gliadin antibodies. The study demonstrates that a 0.016% concentration of the surfactant Tween-20 can induce the spontaneous formation of stable CSMP clusters in dispersion. These clusters, composed of weakly interacting functionalized CSMPs, undergo fragmentation in the presence of gliadin, which specifically binds to the antibodies on the CSMPs. This process results in a colour change, which is measurable by a UV–VIS spectrophotometer. Gliadin extraction was achieved by treating the sample with a non-toxic ethanol-water mixture (60%), sufficient to induce a measurable colour change in the presence of gluten contamination, with a limit of detection (LOD) of 8 ppm, which is lower than low limit established for gluten-free food.</div></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100545"},"PeriodicalIF":10.61,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024001092/pdfft?md5=96b1a1a59d87700aead103072aaa89c9&pid=1-s2.0-S2590137024001092-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142310934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the application of sensor arrays based on nanozymes","authors":"Ying Ma,&nbsp;Heng Liu,&nbsp;Bin Li,&nbsp;Na Lu","doi":"10.1016/j.biosx.2024.100542","DOIUrl":"10.1016/j.biosx.2024.100542","url":null,"abstract":"<div><p>As efficient biocatalysts, enzymes can catalyze various reactions under mild conditions, and have excellent substrate specificity and selectivity. However, the high cost of preparation and difficulty in recovery of natural enzymes have seriously hindered their practical applications. Compared with natural enzymes, the nanozymes with enzyme-like activities have unique advantages in activity regulation, high stability and large-scale preparation. Nanozymes have been widely concerned in the fields of biological detection, medical treatment and cellular immunity, and have a great application potential in the sensor array field. The sensor arrays have recently received an increasing attention due to their applicability in complex biological analysis and diagnosis. This review first summarizes the classification of nanozymes and their corresponding enzyme-like activities. The applications of nanozymes in various sensor arrays are then introduced, in particular, the detecting small molecule, protein and pesticide as nonspecific recognition receptors are addressed. Finally, the future development directions and design principles are prospected for overcoming current challenges, thus providing more opportunities for the application of nanozyme sensor arrays.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100542"},"PeriodicalIF":10.61,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024001067/pdfft?md5=83b7fd0a51e1dded15c6ad7101314a85&pid=1-s2.0-S2590137024001067-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A perspective on the selection and design of nanozyme-based aptasensors for small molecules","authors":"Tanu Bhardwaj,&nbsp;Tarun Kumar Sharma","doi":"10.1016/j.biosx.2024.100533","DOIUrl":"10.1016/j.biosx.2024.100533","url":null,"abstract":"<div><p>Nanozymes and aptamers have long been integral parts of the biosensing field. Recent advancements in these areas have culminated in the creation of a novel class of biosensors known as nanozyme-based aptasensors. In these sensors, aptamers confer specificity to the target analyte, while nanozymes function as transducers, converting a binding event (the binding of the aptamer to its target) into a detectable signal. Despite their promising potential and diverse applications, the detection of small-target molecules, like antibiotics, toxins, metal ions, etc., using nanozyme-based aptasensors remains challenging. This perspective focuses on the obstacles associated with the selection of aptamers for small targets, the design and efficiency of nanozymes, and their integration into functional sensors. In the current perspective, we outline the key challenges and propose various strategies to overcome these hurdles, drawing lessons from past failures to inspire further research for detection of small-target molecules. By incorporating these measures, the performance of nanozyme-based aptasensors to detect small-target molecules can be significantly improved, leading to more effective detection platforms with enhanced sensitivity in the near future.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100533"},"PeriodicalIF":10.61,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000979/pdfft?md5=67adb7fc3d9bd7270f1183b4ecfaec2c&pid=1-s2.0-S2590137024000979-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the performance of glucose oxidase biofuel cell by methyl red and chitosan composite electrodes","authors":"Facheng Su ,&nbsp;Yujyun Wu ,&nbsp;Hsiharng Yang","doi":"10.1016/j.biosx.2024.100534","DOIUrl":"10.1016/j.biosx.2024.100534","url":null,"abstract":"<div><p>This research aims to improve the output power of self-pumping glucose enzymatic biofuel cell (EBFC) and modifying the anode. Adding a fixed ratio of methyl red-chitosan (MR-CS) can effectively improve the EBFC efficiency and stability. In addition, chitosan can be obtained from discarded crustacean fishery waste objects such as shrimp and oysters, are also significant to the use of environmentally friendly materials. The catalyst was immobilized on pyrenecarboxaldehyde (PCA), polyethyleneimine (PEI) and multi-wall carbon nanotubes (MWCNT) and combined with glucose oxidase (GOx). Finally, the [PCA/GOx]/PEI/Nafion solution/MWCNT/[MR-CS] catalyst was immobilized on the carbon cloth. Experimental analysis was progressed under the preparation of enzyme-supported electrode to observe the feasibility of the anode electrode. Experiment including Fourier transform infrared spectroscopy (FTIR) to analyze the distribution of functional groups after modification of the carbon cloth electrode, and through the comparison of the ultraviolet–visible spectrometer (UV–Vis), it can be known that the concentration ratio of [MR-CS] is 1:5, the glucose oxidase load can be maximized. Electrochemical analysis (Cyclic Voltammetry, CV) measures the activity of the maximum reaction of the anode material and the corresponding redox peak, and scanning electron microscope (SEM) observes the surface morphology of the modified electrode. Self-pumping glucose enzymatic biofuel cell module was assembled and examined, the results showed that the maximum output power density (MPD) was 2.64 mW/cm².</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"21 ","pages":"Article 100534"},"PeriodicalIF":10.61,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000980/pdfft?md5=d8c83976a0295526b7046246bb69e4d7&pid=1-s2.0-S2590137024000980-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142232848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peptide-based electrochemical biosensors for the detection of disease biomarkers","authors":"Devika Vinod ,&nbsp;Sandhya Sadanandan ,&nbsp;Rejithamol Rajamani","doi":"10.1016/j.biosx.2024.100531","DOIUrl":"10.1016/j.biosx.2024.100531","url":null,"abstract":"<div><p>The detection of clinically important disease-specific biomarkers such as proteins, nucleic acids, antibodies, enzymes, viruses and circulating tumor cells is essential for understanding their role in disease diagnosis and prognosis. Thus, current clinical research aims at developing a biosensor for the ultrasensitive, reliable, and specific detection of these low-abundant biomolecules in bodily fluids including urine, saliva, and blood. Electrochemical biosensors are powerful devices that make it simple, quick, and affordable the detection of disease biomarkers in clinical diagnostics Peptides epitomize an intriguing group of biorecognition elements that can be linked to electrochemical transducers owing to their stability and selectivity concerning a target analyte. Moreover, they are amenable to facile synthesis and modification with designated functional groups, rendering them appropriate for the creation of innovative architectures for electrochemical biosensing systems. In this review, we provided an outline of the most recent developments in material designs, recognition systems, and strategy advancements related to fabricating peptide-based electrochemical biosensors for disease biomarker detection.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"20 ","pages":"Article 100531"},"PeriodicalIF":10.61,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000955/pdfft?md5=e7cd16cb5d5be215d12d6ecb6ea05738&pid=1-s2.0-S2590137024000955-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142058532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A glassy carbon electrode modified with gold decorated iron oxide/ carbon dots for light assisted voltammetric detection of antibiotic resistant microbe Enterococcus faecalis","authors":"Shobana Babu ,&nbsp;Renugadevi Kathirvel ,&nbsp;Prakash Periakaruppan","doi":"10.1016/j.biosx.2024.100532","DOIUrl":"10.1016/j.biosx.2024.100532","url":null,"abstract":"<div><p>Detecting bacteria is essential in managing significant health concerns as it enables timely intervention, reducing complications and improving patient outcomes, particularly in treating common infections that necessitate precise identification for effective symptom management. Enterococcus species represent a notable threat in hospital-acquired infections and urinary tract infections (UTIs), given the increasing prevalence of strains resistant to multiple antibiotics, unresponsive to standard therapies, and carrying various virulence factors. Traditional approaches to identifying <em>Enterococcus faecalis</em> (<em>E. faecalis</em>) have limitations, including prolonged processing times, limited sensitivity, and the potential for false positive results. While Polymerase Chain Reaction (PCR) is a valuable tool, it is susceptible to contamination and variations in DNA concentration. The emerging technique of Photoelectrochemical (PEC) holds promise for enhancing <em>E. faecalis</em> detection by leveraging photogenerated electrons and holes. This study introduces a rapid and precise approach utilizing a light-assisted electrochemical biosensor featuring a glassy carbon electrode modified with a nanocomposite of gold-coated iron oxide and carbon dots (Au@Fe₃O₄/CDs). The nanocomposite was successfully synthesized and underwent thorough characterization. The investigation has a detection range from 1 to 14 CFU mL⁻¹, along with a notably low limit of detection (LOD: 3 CFU mL⁻¹, LOQ: 10 CFU mL⁻¹). Rigorous examination of real-world samples such as food, water, and soil demonstrated exceptional specificity, reproducibility, and long-term stability of the sensor. The applications of the Au@Fe₃O₄/CDs nanocomposite in PEC processes underscore the potential of this innovative approach in addressing health concerns associated with bacterial infections and delivering real-time impacts for both healthcare and environmental domains.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"20 ","pages":"Article 100532"},"PeriodicalIF":10.61,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000967/pdfft?md5=34de78549f37917f1dd49d1a65e225d3&pid=1-s2.0-S2590137024000967-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142040019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced graphene oxide based ultrasensitive resistive sensor for detection of CA125","authors":"Bolivia Konthoujam ,&nbsp;Nikita Bhandari ,&nbsp;Miriyala Pranay Kamal ,&nbsp;P. Nitin Srinivas ,&nbsp;Bhanoday Thati ,&nbsp;Pranav Bondugula ,&nbsp;Purushotham Reddy ,&nbsp;Ramalingappa C. Antaratani ,&nbsp;Naveen Kadayinti ,&nbsp;Sudhanshu Shukla ,&nbsp;Ruma Ghosh","doi":"10.1016/j.biosx.2024.100530","DOIUrl":"10.1016/j.biosx.2024.100530","url":null,"abstract":"<div><p>Early-stage detection of any cancer significantly improves the survival rates by enabling clinicians to design simpler and more effective treatment options, leading to a cure or remission. Early diagnosis of ovarian cancer, the leading cause of gynaecological cancer related mortalities, relies heavily on accurate detection of the serum biomarker CA125. This work presents a simple rGO/monoclonal antibody (mAB)/bovine serum albumin (BSA) based 2-port resistive sensor for CA125. The binding of mAB on rGO was confirmed by atomic force microscopy which showed increase in thickness of the device from 1.4 nm to approximately 40–60 nm after the mAB anchored on the device. FESEM further confirmed the morphologies of rGO, rGO/mAB, and rGO/mAB/CA125. The sensor exhibited impressive response ranging from 1.28% to 113.4% for 1 pg/mL to 300 ng/mL CA125. Notably, the rGO/mAB/BSA sensor displayed high selectivity towards CA125 and a readout circuit was designed, assembled, and tested with the sensors to get a portable device for detecting CA125. The developed sensors were tested with 9 clinical samples and were found to be determining the CA125 concentration accurately.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"20 ","pages":"Article 100530"},"PeriodicalIF":10.61,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000943/pdfft?md5=f54c47c1763ed32e9d8b79a9cf6128c0&pid=1-s2.0-S2590137024000943-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142048991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key aspects of biosensing for instant screening tests","authors":"Joydip Sengupta","doi":"10.1016/j.biosx.2024.100529","DOIUrl":"10.1016/j.biosx.2024.100529","url":null,"abstract":"<div><p>The landscape of biosensing technologies has undergone a significant transformation, with a particular emphasis on instant screening tests (ISTs) tailored for home and community settings. These tests play a crucial role in enabling rapid detection, monitoring, and management of a varied range of health conditions, including infectious diseases, chronic illnesses, and environmental exposures. This letter provides a brief exploration of the various key aspects of biosensing methodologies and technologies designed to address the unique challenges and opportunities inherent in ISTs. By exploring crucial advancements and emerging trends, it highlights the transformative potential of these innovations in enhancing healthcare accessibility and empowering individuals to take proactive control of their well-being.</p></div>","PeriodicalId":260,"journal":{"name":"Biosensors and Bioelectronics: X","volume":"20 ","pages":"Article 100529"},"PeriodicalIF":10.61,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2590137024000931/pdfft?md5=eaff6d83e3ce1da954e472a4f0a3eea4&pid=1-s2.0-S2590137024000931-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141992647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}